IVF0000766 (gene) Melon (IVF77) v1

Overview
NameIVF0000766
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description4-coumarate--CoA ligase-like 1
Locationchr10: 4016322 .. 4019287 (-)
RNA-Seq ExpressionIVF0000766
SyntenyIVF0000766
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGTTTGGTTCCAAGGAATATAGGAGACTGCCAAGACCAACTTGAGATGTTATGGAGGGCTTCGTTTTTTCAAAGAATTTTATGATGGGTTGCTTCCAACAATAATCAGTAAACAAATAACAGAGATAAAATCATGTTTTAAATAATGTAATTCTATATGGTTCAATTAATTTTAAAGCAAGCTAGCTTCTGTTTATAAAGAGAGCTTAGCTTAAGACTGCCTGAATGAATGGCATGAATCAACAATATCCAACCAAAAAAAAGGCTCATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGATCCATGTCGAAGCCGAAAACTACTACTCCGCCATGACTACTCGCAACCAAGATTCGGTACAAGACGAGAAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTTGTGATATCACACTGCCTGAGTTTGTACTACAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTCTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCACTCTCTGAGGTTGAAGAAAGGGCATGTGGTTCTTGTTGTTCTACCTAATGTTGCAGAATACGCGATTGTTGCTTTAGGGATAATGACTGCTGGAGGTGTTTTTTCGGGAGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTTGTCACAAATGCCGCAAACTTCGAAAAGGTAGGATAGAATTAAAAACAATCGTTGATAACTTGATACGGAACATATTTCTTTTGAGTTCACCGCGTCTCACTGACTGTCTATGTTGTTGAGGACAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGCAACAATTTTGTTAAAGAAGAGATTAAGCAAACTGATCTATGTGCCCTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCCGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACACTAGGCCTTATTCCGTTCTTCCATATTTATGGGATCACTGGCATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATCCTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATTATGACTGCAGCTGCTCCACTCGCACCTGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCTGGCGTGGACGTTCAAGAGGTAACTCAGTTGTTACTCGAGTGTGGTAGATTGACTGTGACAGCTCTCCATTCTTTTTTCATTTCTTCAATTTTTTTAGTTATGCCATATAGTCCAACTTCCTGTTTTCGATATGTGAGAAGCAAATCAAGCTCTACCGTCACGTTGCAGAGTAAATCTTTGGGGTTCTAACTTCAATAATATGCCAACTAATTGTTGTTCATCATCAACAGGCATATGGACTTACTGAGCACTGCTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATAGACCCGGACACTGGAAGGTCTCTGCCGAAGAACACGCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGTACAGAACATAGTTCGATGCAGAAATTTTTTATGGGATCTCTAATTAAATGCCAGCTGAAGCTCTCCTTCTTGTTTCAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATAGCAAAGGCTGGATGCATACTGGGGATATTGGATACATCGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGCTTTCAAGTAAAGACATAACATAGCTTATTTCATATTGATCCATTGGTTGATAAAAAGGAAGCTGAAGATGTGAAAAGTGGAAGTTTGCATCAAACTTCTTTTTAGGTGTTTTTTGTATATATGAATATGAAATAAAGTGATGGATGTCATTGATTTCTCTCTAGGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTAAGATTTCTCGCCTCGTTCATTTCGCTTCGTGTTGTTTCAATCTAAAGATAACAATGCTGTGCTGCTTTGGCATTTCAGGCTGCCAGATGAAGAAGCTGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAACGAAACCGAAGAAGAAATAATCAAACACGTTGCCTCAAATGTTGCACATTATAAGAAAGTTAGACTCGTTCATTTTGTAGACACCATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGAATTGTCTTCTAACCTTTTTTAGTTGACTGAGAGATGTTCGAATTAAACTGTCATGTCTATATTTTACAAGATTTAGAAGATGCTATCTTAATATCAAGTCTACTTGATCCATTGCTTTTAGAGTTACTTAAATTGTTGTATTTTCTCAGTTTAATGTCTAAGTAGGTGTCAGGTCTAAAGATTTAATCTGTTCCTTATTTCTATGTTTATCAATTTCCATTTTCAATGTTGCTTTAAATTGTGAGTTAGAAGTACTAAATATTAATTAGGTTCATTTTAAAGGCAAAAC

mRNA sequence

GTTGTTTGGTTCCAAGGAATATAGGAGACTGCCAAGACCAACTTGAGATGTTATGGAGGGCTTCGTTTTTTCAAAGAATTTTATGATGGGTTGCTTCCAACAATAATCAGTAAACAAATAACAGAGATAAAATCATGTTTTAAATAATGTAATTCTATATGGTTCAATTAATTTTAAAGCAAGCTAGCTTCTGTTTATAAAGAGAGCTTAGCTTAAGACTGCCTGAATGAATGGCATGAATCAACAATATCCAACCAAAAAAAAGGCTCATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGATCCATGTCGAAGCCGAAAACTACTACTCCGCCATGACTACTCGCAACCAAGATTCGGTACAAGACGAGAAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTTGTGATATCACACTGCCTGAGTTTGTACTACAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTCTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCACTCTCTGAGGTTGAAGAAAGGGCATGTGGTTCTTGTTGTTCTACCTAATGTTGCAGAATACGCGATTGTTGCTTTAGGGATAATGACTGCTGGAGGTGTTTTTTCGGGAGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTTGTCACAAATGCCGCAAACTTCGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGCAACAATTTTGTTAAAGAAGAGATTAAGCAAACTGATCTATGTGCCCTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCCGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACACTAGGCCTTATTCCGTTCTTCCATATTTATGGGATCACTGGCATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATCCTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATTATGACTGCAGCTGCTCCACTCGCACCTGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCTGGCGTGGACGTTCAAGAGGCATATGGACTTACTGAGCACTGCTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATAGACCCGGACACTGGAAGGTCTCTGCCGAAGAACACGCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATAGCAAAGGCTGGATGCATACTGGGGATATTGGATACATCGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGCTGCCAGATGAAGAAGCTGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAACGAAACCGAAGAAGAAATAATCAAACACGTTGCCTCAAATGTTGCACATTATAAGAAAGTTAGACTCGTTCATTTTGTAGACACCATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGAATTGTCTTCTAACCTTTTTTAGTTGACTGAGAGATGTTCGAATTAAACTGTCATGTCTATATTTTACAAGATTTAGAAGATGCTATCTTAATATCAAGTCTACTTGATCCATTGCTTTTAGAGTTACTTAAATTGTTGTATTTTCTCAGTTTAATGTCTAAGTAGGTGTCAGGTCTAAAGATTTAATCTGTTCCTTATTTCTATGTTTATCAATTTCCATTTTCAATGTTGCTTTAAATTGTGAGTTAGAAGTACTAAATATTAATTAGGTTCATTTTAAAGGCAAAAC

Coding sequence (CDS)

ATGCCAATCTCACCCTCAAGAACTCTACAAGTTTCTCATTCACAAAGCATGATACAACCTTTCTCAACTCTTCTTTTAAGTAGAGGGAAGTTGATCCATGTCGAAGCCGAAAACTACTACTCCGCCATGACTACTCGCAACCAAGATTCGGTACAAGACGAGAAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTTGTGATATCACACTGCCTGAGTTTGTACTACAGAATGCTGAATCATATGCCGAAAATGTGGCATTCGTGGAAGCCATGAGCGGAAAGGCATACACTTACCGTGAAGTCTTAAGAGACACAAATAGATTTTCCAAGGCCTTGCACTCTCTGAGGTTGAAGAAAGGGCATGTGGTTCTTGTTGTTCTACCTAATGTTGCAGAATACGCGATTGTTGCTTTAGGGATAATGACTGCTGGAGGTGTTTTTTCGGGAGTGAATCCGGCAGCTCACATATCAGAAATCAAAAAGCAAGTGGAGGTAGCAGAAGCCAAACTTGTTGTCACAAATGCCGCAAACTTCGAAAAGGTGAGGGAATTAAAGCTACCAGTCATCTTATTAGAGGAAGAACTAATGGAAGGTGCCATGAATTGGCACAAACTTCTTGAAGCAGCAGATCGTGCAGGCAACAATTTTGTTAAAGAAGAGATTAAGCAAACTGATCTATGTGCCCTTCCTTTCTCATCAGGAACCACAGGAGTTTCCAAAGGTGTTATGTTAACTCACCGAAATCTCGTAGCTAATATGTGTTCGACTCTCTCCGGTGTCCCGCAAGAAATGGAGGGCAAGGTCACGACACTAGGCCTTATTCCGTTCTTCCATATTTATGGGATCACTGGCATATGTTGTTCTACACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACTTTCATTAATGCTCTCATAACACATGAGATCACATTTGCTCCAATTGTTCCTCCTATTATCCTGGCTTTGGTTAAGAATCCCATTGTGGAAGAATTTGATCTTAGCAGTCTCAAACTTCAGGCTATTATGACTGCAGCTGCTCCACTCGCACCTGAACTTCAAACTGCCTTCGAGAGAAAGTTCCCTGGCGTGGACGTTCAAGAGGCATATGGACTTACTGAGCACTGCTGCATCACTCTTAACTATGGAAATATAGGGAAAGAGAATCTCAGTGCAAAGAAAAACACAGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATAGACCCGGACACTGGAAGGTCTCTGCCGAAGAACACGCCGGGTGAAATCTGTGTAAGAAGCCAATGTGTGATGCAAGGCTACTACAAAAACGAAGAGGAGACTTCAAGGACGATCGATAGCAAAGGCTGGATGCATACTGGGGATATTGGATACATCGATGACGATGGCGACGTGTTTATTGTGGATCGTATCAAGGAATTGATCAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTTCTAACTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGCTGCCAGATGAAGAAGCTGGTGAGATCCCAGCAGCAAGCGTTGTGATGGCTCCGAACTCAAACGAAACCGAAGAAGAAATAATCAAACACGTTGCCTCAAATGTTGCACATTATAAGAAAGTTAGACTCGTTCATTTTGTAGACACCATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCTGATGAATCCGCTAGAATTGTCTTCTAA

Protein sequence

MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF
Homology
BLAST of IVF0000766 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 9.4e-224
Identity = 386/532 (72.56%), Postives = 453/532 (85.15%), Query Frame = 0

Query: 52  QDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRF 111
           +D ++IFRS  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GKA TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 112 SKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEA 171
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 172 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 231
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 232 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 291
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 292 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 351
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 352 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 411
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G+  K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 412 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYID 471
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 472 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 531
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 532 APNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 584
            P + E EE+I+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of IVF0000766 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 696.8 bits (1797), Expect = 2.1e-199
Identity = 345/539 (64.01%), Postives = 437/539 (81.08%), Query Frame = 0

Query: 52  QDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMS-GKAYTYREVLRDTNR 111
           ++++H+FRS+ P V VP  +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RDT R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 112 FSKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAE 171
           F++AL S+ ++KGHVV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE +E
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 172 AKLVVTNAANFEKVRELKLPVI-LLEEELMEGAMNWHKLLEAADRAGNNFVK-EEIKQTD 231
           AKLVV N   F+KV++  +PVI + + E M GA++W  LL AADR G   V  +  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 232 LCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGI 291
           LCALP+SSGTTGVSKGVML+HRNLV+N+CS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 292 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 351
           CC+TLR+KG VVVM RFDLRTF+ AL+ H + FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 352 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGR 411
           +++MTAAAPLAP+L  AF+RKFPGV V+EAYGLTEH CITL +         AKK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 412 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIG 471
           ILPNLEVKF+DPDTGRSLP NTPGE+CVRSQ VMQGYYK +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 472 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 531
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 532 VVMAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIR 588
           VV    + E EEEI+ +VA  VA YK+VR++H VD IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of IVF0000766 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.6e-114
Identity = 204/531 (38.42%), Postives = 330/531 (62.15%), Query Frame = 0

Query: 57  IFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALH 116
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + +  L+
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 117 SLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 176
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 177 NAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALP 236
            A    KV++  +     ++  + + EG +++ +L+++ +   +     +I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDE---HEIPDVKIQPDDVVALP 193

Query: 237 FSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCS 296
           +SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 297 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 356
            LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V+ +DLSS++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--TV 313

Query: 357 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 416
           M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++ 
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVR 373

Query: 417 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYID 476
           N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+HTGDIG+ID
Sbjct: 374 NAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFID 433

Query: 477 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 536
           DD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV 
Sbjct: 434 DDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVR 493

Query: 537 APNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           +  S  TE+E+   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 494 SNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of IVF0000766 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.0e-113
Identity = 211/534 (39.51%), Postives = 331/534 (61.99%), Query Frame = 0

Query: 54  EKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSK 113
           E  IFRS+LP++ +P  + L  +  +N     +    +   +G+ +TY +V   + + + 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 114 ALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKL 173
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ ++AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 174 VVTNAANFEKVREL---KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLC 233
           ++T A   +KV++    K   I+  ++  +  +++ KL+EA +   +   +  I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADE---SEMPEVVINSDDVV 188

Query: 234 ALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGI 293
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 294 CCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKL 353
            C  LR    +++M +FD+  F+  +  +++T  P VPPI+LA+ K+P+V+++DLSS++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 354 QAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGR 413
             +M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 414 ILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIG 473
           ++ N E+K +DP+T  SLP+N  GEIC+R   +M+GY  + E T  TID +GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 474 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAAS 533
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ DE+AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 534 VVMAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           VV       TEEEI + V+  V  YK++  V FVD IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

BLAST of IVF0000766 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.4e-113
Identity = 204/531 (38.42%), Postives = 328/531 (61.77%), Query Frame = 0

Query: 57  IFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALH 116
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + +  L+
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 117 SLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 176
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 177 NAANFEKVRELKLP---VILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALP 236
            A    KV++  +     ++  +   EG +++ +L+++ +   +     +I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDE---HEIPDVKIQPDDVVALP 193

Query: 237 FSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCS 296
           +SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 297 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 356
            LR    +++M +FD+  F+  +  H++T  P VPPI+LA+ K+P+V  +DLSS++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSVR--TV 313

Query: 357 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 416
           M+ AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++ 
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVR 373

Query: 417 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYID 476
           N E+K +DPDTG SLP+N PGEIC+R   +M+GY  + E T+RTI+ +GW+HTGDIG+ID
Sbjct: 374 NAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFID 433

Query: 477 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 536
           DD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV 
Sbjct: 434 DDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVR 493

Query: 537 APNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           +  S  TE+E+   ++  V  YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 494 SNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of IVF0000766 vs. ExPASy TrEMBL
Match: A0A1S3BER3 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 597
           EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

BLAST of IVF0000766 vs. ExPASy TrEMBL
Match: A0A5D3CV41 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002330 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 17  MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 76
           MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 597
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

BLAST of IVF0000766 vs. ExPASy TrEMBL
Match: A0A0A0KRZ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 3.9e-305
Identity = 539/554 (97.29%), Postives = 546/554 (98.56%), Query Frame = 0

Query: 43  MTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 102
           M T NQDSVQDE+HIF SQLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGKAYTYR
Sbjct: 1   MATCNQDSVQDEEHIFCSQLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYR 60

Query: 103 EVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEI 162
           EVLRDTNRFSKAL SLRLKKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEI
Sbjct: 61  EVLRDTNRFSKALRSLRLKKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 163 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 222
           KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE
Sbjct: 121 KKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE 180

Query: 223 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 282
           EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI
Sbjct: 181 EIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 283 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 342
           YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFD 300

Query: 343 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAK 402
           LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAK
Sbjct: 301 LSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAK 360

Query: 403 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWM 462
           KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWM
Sbjct: 361 KNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWM 420

Query: 463 HTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 522
           HTGDIGYIDDDG+VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG
Sbjct: 421 HTGDIGYIDDDGNVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAG 480

Query: 523 EIPAASVVMAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 582
           EIPAASVVMAPNS ETEE+IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM
Sbjct: 481 EIPAASVVMAPNSKETEEDIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 540

Query: 583 IEKIRADESARIVF 597
           IEKIRADESAR +F
Sbjct: 541 IEKIRADESARFIF 554

BLAST of IVF0000766 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1022.7 bits (2643), Expect = 6.2e-295
Identity = 517/592 (87.33%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  S M T  +DS +DE+HIFRS
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRS 74

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVP DITLPEFVLQNAE YA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG +V+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSG+PQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLSSLKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGNIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+ ETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 593
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of IVF0000766 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 8.1e-295
Identity = 519/592 (87.67%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  SAM T  +D V+DE+HIFRS
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+ ETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 593
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of IVF0000766 vs. NCBI nr
Match: XP_008446022.1 (PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo])

HSP 1 Score: 1167 bits (3019), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596
           EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596

BLAST of IVF0000766 vs. NCBI nr
Match: KAA0034187.1 (4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1137 bits (2941), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 17  MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 76
           MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF
Sbjct: 1   MIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEF 60

Query: 77  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 136
           VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY
Sbjct: 61  VLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEY 120

Query: 137 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 196
           AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE
Sbjct: 121 AIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEE 180

Query: 197 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 256
           ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM
Sbjct: 181 ELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 240

Query: 257 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 316
           CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALIT 300

Query: 317 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 376
           HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ
Sbjct: 301 HEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQ 360

Query: 377 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 436
           EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEICV 420

Query: 437 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 496
           RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 497 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 556
           ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYKKV 540

Query: 557 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596
           RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF
Sbjct: 541 RLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 580

BLAST of IVF0000766 vs. NCBI nr
Match: XP_004135516.2 (4-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical protein Csa_008120 [Cucumis sativus])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 577/596 (96.81%), Postives = 584/596 (97.99%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL    AENYYSAM T NQDSVQDE+HIF S
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKL--TPAENYYSAMATCNQDSVQDEEHIFCS 60

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVP DITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKAL SLRL
Sbjct: 61  QLPEVQVPYDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALRSLRL 120

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKGHVV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN
Sbjct: 121 KKGHVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQTAFERKFPGVDVQEAYGLTEHCCITLNYG+IGKENLSAKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGSIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTID+KGWMHTGDIGYIDDDG+VFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDTKGWMHTGDIGYIDDDGNVFIVD 480

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS ETEE
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEE 540

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARIVF 596
           +IIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESAR +F
Sbjct: 541 DIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESARFIF 594

BLAST of IVF0000766 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1024 bits (2648), Expect = 0.0
Identity = 517/592 (87.33%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  S M T  +DS +DE+HIFRS
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATCIRDSAEDEEHIFRS 74

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVP DITLPEFVLQNAE YA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG +V+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSG+PQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLSSLKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYGNIGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID+KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+ ETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 592
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 606

BLAST of IVF0000766 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 519/592 (87.67%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  SAM T  +D V+DE+HIFRS
Sbjct: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDLVEDEEHIFRS 74

Query: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134

Query: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180
           KKG VV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194

Query: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240
           FEKV+ELKLPVI++ EEL+EG+MNWHKLLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 195 FEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254

Query: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314

Query: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374

Query: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434

Query: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494

Query: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VMAPN+ ETE+
Sbjct: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMAPNAKETED 554

Query: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 592
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAEAAS 606

BLAST of IVF0000766 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 777.7 bits (2007), Expect = 6.7e-225
Identity = 386/532 (72.56%), Postives = 453/532 (85.15%), Query Frame = 0

Query: 52  QDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRF 111
           +D ++IFRS  P V +P  +TLPEFVLQ  E Y ENVAFVEA++GKA TY +V+RDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 112 SKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEA 171
           +KAL SL L+KG V++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 172 KLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCA 231
           + ++T+A N+EKV+ L LPVI+L EE +EGA+NW  LLEA D+ G+    EEI QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 232 LPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCS 291
           LPFSSGTTG+ KGVMLTHRNL+AN+CSTL GV  EM G++ TLGLIPFFHIYGI GICC+
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 292 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 351
           T++NKGKVV M R+DLR F+NALI HE++FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 352 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 411
           MTAAAPLAPEL TAFE KFP V VQEAYGLTEH CITL +G+  K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 412 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYID 471
           NLEVKFIDPDTGRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID +GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 472 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 531
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPDEEAGEIPAA VV+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 532 APNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMI 584
            P + E EE+I+  VA+NVAHYKKVR VHFVD+IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of IVF0000766 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 403.7 bits (1036), Expect = 2.6e-112
Identity = 204/532 (38.35%), Postives = 330/532 (62.03%), Query Frame = 0

Query: 57  IFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALH 116
           IFRS+LP++ +P  + L +++ +N   +A     +   +G+ YTY +V   + + +  LH
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 117 SLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 176
           +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 177 NAANFEKVRELKLPVILL----EEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCAL 236
            +   +K++ L+   +L+     + + E  + + +L ++ +   ++ + E+I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 237 PFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEM--EGKVTTLGLIPFFHIYGITGICC 296
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 297 STLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQA 356
            +LR    +++M +F++   +  +   ++T A +VPPI+LA+ K+P  E++DLSS+++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 357 IMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRIL 416
           + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    G ++
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 417 PNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYI 476
            N E+K +DPDTG SLP+N PGEIC+R   +M+GY  +   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 477 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVV 536
           DDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A VV
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 537 MAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
            + +SN +E+EI + V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of IVF0000766 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 392.1 bits (1006), Expect = 7.9e-109
Identity = 201/536 (37.50%), Postives = 320/536 (59.70%), Query Frame = 0

Query: 57  IFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALH 116
           IFRS+LP++ +P  ++L +++ QN   +A     +   +G  YTY +V   + + +   H
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 117 SLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 176
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + +  KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 177 NAANFEKVRELK----LPVILLEEE----LMEGAMNWHKLLEAADRAGNNFVKEEIKQTD 236
            A   +K++ L+    + ++ +++     + EG + + +L ++   A       EI   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 237 LCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEM--EGKVTTLGLIPFFHIYGIT 296
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 297 GICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSL 356
            I    LR    +++M +F++   +  +   ++T AP+VPPI+LA+ K+   E++DLSS+
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 357 KLQAIMTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTV 416
           ++  + + AAPL  EL+ A   KFP   + + YG+TE   +        KE    K    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 417 GRILPNLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGD 476
           G ++ N E+K +DPDTG SL +N PGEIC+R   +M+GY  N   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 477 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPA 536
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + +E AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 537 ASVVMAPNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           A VV + +S  +E+++ + V+  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of IVF0000766 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 386.0 bits (990), Expect = 5.6e-107
Identity = 204/531 (38.42%), Postives = 326/531 (61.39%), Query Frame = 0

Query: 57  IFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALH 116
           IFRS+LP++ +P  + L  +  +   S ++    +   +GK+YTY E      R +  L+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 117 SLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 176
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 177 NAANFEKVREL--KLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKE-EIKQTDLCALP 236
           ++   +K++ L   L +I  +E   E  + +  L+   D   N F +  +I   D  ALP
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI--TDDETNPFQETVDIGGDDAAALP 213

Query: 237 FSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQE--MEGKVTTLGLIPFFHIYGITGICCS 296
           FSSGTTG+ KGV+LTH++L+ ++   + G      ++     L ++P FHIY +  +  +
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 297 TLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAI 356
           +LR+   V++M +F++   ++ +  H +T A +VPP+++AL KNP V  +DLSS++   +
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--V 333

Query: 357 MTAAAPLAPELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILP 416
           ++ AAPL  ELQ +  R+ P   + + YG+TE   +        KE +  K  + G ++ 
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 417 NLEVKFIDPDTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYID 476
           N E+K +  +T  SL  N PGEIC+R Q +M+ Y  + E TS TID +GW+HTGDIGY+D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 477 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVM 536
           +D ++FIVDR+KE+IK+KGFQV PAELE++L+ H SI DAAVVP  DE AGE+P A VV 
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 537 APNSNETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           +  ++ TEE++ ++VA  V  YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of IVF0000766 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 382.5 bits (981), Expect = 6.2e-106
Identity = 198/568 (34.86%), Postives = 337/568 (59.33%), Query Frame = 0

Query: 34  VEAENYYSAMTTRNQDSVQDEKH--IFRSQLPEVQVPCDITLPEFVLQ----NAESYAEN 93
           ++ + ++       +D  ++  H  IFRS+LP++ +P  + L ++V Q    + +  +  
Sbjct: 3   LQQQTHFLTKKIDQEDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSST 62

Query: 94  VAFVEAMSGKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAG 153
              ++  +G+  TY +V  +  R +  +H L ++ G VV+++LPN  E+A+  L +   G
Sbjct: 63  TCIIDGATGRILTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLG 122

Query: 154 GVFSGVNPAAHISEIKKQVEVAEAKLVVTNAANFEKVRELK---LPVILLEEE------- 213
            V +  NP     EI KQ + + AK+++T     +K+  LK   + ++ L+++       
Sbjct: 123 AVSTTANPFYTQPEIAKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVS 182

Query: 214 -LMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANM 273
              +G +++ +L +A +      +K +I   D  A+P+SSGTTG+ KGVM+TH+ LV ++
Sbjct: 183 SSDDGCVSFTELTQADE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSI 242

Query: 274 CSTLSGVPQEME--GKVTTLGLIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINAL 333
              + G    +        L  +P FHIY +  +  S +R    ++++ RF+L   +  +
Sbjct: 243 AQKVDGENPNLNFTANDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELI 302

Query: 334 ITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVD 393
             +++T  P+ PP++LA +K+P  E +DLSS+++  +++ AA L  EL+ A   KFP   
Sbjct: 303 QRYKVTVVPVAPPVVLAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAI 362

Query: 394 VQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDPDTGRSLPKNTPGEI 453
             + YG+TE   +  +     K     K    G ++ N E+K +D +TG SLP+N  GEI
Sbjct: 363 FGQGYGMTESGTVAKSLA-FAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEI 422

Query: 454 CVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVA 513
           CVR   +M+GY  + E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVA
Sbjct: 423 CVRGHQLMKGYLNDPEATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVA 482

Query: 514 PAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEEEIIKHVASNVAHYK 573
           PAELEA+L++HPSI+DAAVV + DE A E+P A V  +  S  TE+++  +V   V HYK
Sbjct: 483 PAELEALLISHPSIDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYK 542

Query: 574 KVRLVHFVDTIPKSPSGKVMRRLIKEKM 583
           ++++V F++ IPK+ SGK++R+ ++ K+
Sbjct: 543 RIKMVFFIEVIPKAVSGKILRKDLRAKL 564

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LQ129.4e-22472.564-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL22.1e-19964.014-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P316844.6e-11438.424-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
P149121.0e-11339.514-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
P316851.4e-11338.424-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BER30.0e+00100.004-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1[more]
A0A5D3CV410.0e+00100.004-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0KRZ53.9e-30597.29Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G589320 PE=4 SV=1[more]
A0A6J1HUV56.2e-29587.334-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A6J1G0B48.1e-29587.674-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
Match NameE-valueIdentityDescription
XP_008446022.10.0100.00PREDICTED: 4-coumarate--CoA ligase-like 1 [Cucumis melo][more]
KAA0034187.10.0100.004-coumarate--CoA ligase-like 1 [Cucumis melo var. makuwa] >TYK15733.1 4-coumarat... [more]
XP_004135516.20.096.814-coumarate--CoA ligase-like 1 [Cucumis sativus] >KAE8648676.1 hypothetical prot... [more]
XP_022966799.10.087.334-coumarate--CoA ligase-like 1 [Cucurbita maxima][more]
XP_022945246.10.087.674-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G62940.16.7e-22572.56acyl-CoA synthetase 5 [more]
AT3G21240.12.6e-11238.354-coumarate:CoA ligase 2 [more]
AT1G51680.17.9e-10937.504-coumarate:CoA ligase 1 [more]
AT1G65060.15.6e-10738.424-coumarate:CoA ligase 3 [more]
AT3G21230.16.2e-10634.864-coumarate:CoA ligase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 497..572
e-value: 5.8E-18
score: 65.7
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 55..483
e-value: 7.3E-133
score: 445.4
NoneNo IPR availableGENE3D3.30.300.30coord: 484..586
e-value: 9.8E-32
score: 111.7
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 49..585
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 49..585
NoneNo IPR availableCDDcd059044CLcoord: 67..576
e-value: 0.0
score: 685.12
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 50..585
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 79..488
e-value: 9.2E-98
score: 327.6
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 232..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000766.1IVF0000766.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity