IVF0000713 (gene) Melon (IVF77) v1

Overview
NameIVF0000713
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPectinesterase
Locationchr09: 17163524 .. 17165779 (-)
RNA-Seq ExpressionIVF0000713
SyntenyIVF0000713
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATCTTTGTGGTGGGAAAGGTAATTCTTTCATTTGGGTTTTGGTCATTTGAACGACCAAGAAACCTTTTCATATTACCATAAAGGTGGAAACCAAAGAAGAAGAAAAAATTAAAAGAATGGTCAATAAGGTGATCTTGCCTGGGATATCGCTTATTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTCACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCGACCATTTGTGCTAATGCTAATTATCAACAGGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAGAGCAGCAATTCTTTCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACCGATGGATTCATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCAGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTACTCTACTGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAAACAGAAGATGCAAGATGGTCTTTCTGATGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGTAATTCTGCTAGATCACTTACTAATTAATCTCCTCTACCCTACTTCCATTAAATATATTACAATTTTGTTGCAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGGGAAAGATGGATACCCGACATGGTTGACTGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTACAATGAATATGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGACACTGCCACTTTCAGTAAGTGAATGAACTTACCACAAGCCAAATATGATCGGACAATTTGATGGAATGCACTCACTATTTTTCTTTCAACTTACACGTATATTTATACTTGTAGTTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCATCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAACGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGTTGGATACATGCCCTGGTCTGGAAATTTTGCACTGGAGACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGTGCTGTCACCAATAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATTGGAAGAAATGAAGCCATGCAGTTCACTGCTGGACCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCCTATCTATTGGGATTGAAAAGCTAAAAGAAAGAATTGTGTTTGTTTGATGACGAATGTAATATAATTTATTTGTTCATTCCCCATTCTACATCAATTTATTGCCTTAGATTTGAGAAGCAACTCCAATGGCCTTAAAATGGTTACAACTTTCAGAAAGGTGTGCATTATGTAATGAGGAGGTTTAGCAACGTATATAACATTCTTTTTACCATTTTTTAATCGTTACAAAAAGTAAGACTTATTCTTCATTTTGTGATTAATGTCGTAAAAAACCAAACATGC

mRNA sequence

CCCATCTTTGTGGTGGGAAAGGTAATTCTTTCATTTGGGTTTTGGTCATTTGAACGACCAAGAAACCTTTTCATATTACCATAAAGGTGGAAACCAAAGAAGAAGAAAAAATTAAAAGAATGGTCAATAAGGTGATCTTGCCTGGGATATCGCTTATTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTCACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCGACCATTTGTGCTAATGCTAATTATCAACAGGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAGAGCAGCAATTCTTTCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACCGATGGATTCATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCAGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTACTCTACTGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAAACAGAAGATGCAAGATGGTCTTTCTGATGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGGGAAAGATGGATACCCGACATGGTTGACTGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTACAATGAATATGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGACACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCATCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAACGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGTTGGATACATGCCCTGGTCTGGAAATTTTGCACTGGAGACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGTGCTGTCACCAATAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATTGGAAGAAATGAAGCCATGCAGTTCACTGCTGGACCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCCTATCTATTGGGATTGAAAAGCTAAAAGAAAGAATTGTGTTTGTTTGATGACGAATGTAATATAATTTATTTGTTCATTCCCCATTCTACATCAATTTATTGCCTTAGATTTGAGAAGCAACTCCAATGGCCTTAAAATGGTTACAACTTTCAGAAAGGTGTGCATTATGTAATGAGGAGGTTTAGCAACGTATATAACATTCTTTTTACCATTTTTTAATCGTTACAAAAAGTAAGACTTATTCTTCATTTTGTGATTAATGTCGTAAAAAACCAAACATGC

Coding sequence (CDS)

ATGGTCAATAAGGTGATCTTGCCTGGGATATCGCTTATTCTTGTTGTTGGTGTAGCACTTGCTGTTGTTGCAATAGTTCACAGCAACAAGAGTTCTGATGCTGCAACAGAGGATTTATCCCCAAAAATGAAGGCAGTTTCGACCATTTGTGCTAATGCTAATTATCAACAGGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCATCAGATGACCCAAAAGAGTATGTTAGAGCAGCAATTCTTTCCACTATTGAAGAGGTTAAGAAGGGGTATAATTTGACCGATGGATTCATGATTGAGGCTGCTAACAATCGTAGCATTAAAATGGGTGTTGAGGATTGCAGGGACTTGTTGCAATTTGCTATTGATCAGTTGCAAGCATCTTACTCTACTGTTGGTGAACCTGATTTGCATACCAATGCCGATCGTGTCGCAGATATCAAGAATTGGCTAACCTCTGTTATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCTCAGCTTAAACAGAAGATGCAAGATGGTCTTTCTGATGCTGGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGATATTCTTGCAAGCTTTGGCTTGCAATTGAAGGCTCAACCAAGCGGTCGCAGGCTTCTTGGAACAACAGAGGTAGGGAAAGATGGATACCCGACATGGTTGACTGGGGCAGACAGGAAGTTGCTCGCATCAAGAGGAGGAGGCGCTAAAGTGAAACCAAACGCAGTGGTGGCTAAGGACGGTAGCGGACAGTTCAAAACCATTTCTGCTGCATTGGCTGCATATCCAAAGGCCTTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTACAATGAATATGTCCATATCACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAGAAAACCATTGTCACCGGTAACAAGAGCAACCGTGGTGGCTTCACCACCCAAGACACTGCCACTTTCATTGCCATTGGAGAAGGATTCCTTTGCAAATCGATGGGATTCCAAAACACAGCCGGTCCTGAAGGCCACCAAGCAGTAGCTCTTAGAGTCCAATCCGACAGATCTGCCTTCTTTAATTGTAGAATGGATGGCTACCAAGACACTCTCTACGTACAAACTCAACGTCAATTCTACCGTAACTGTGTCATATCCGGCACTGTCGATTTCATCTTTGGCGATTCAACCACCATCATCCAAAACTCCTTGATCATCGTTCGTCGCCCCATGGACAACCAACAGAACACCGTCACAGCCAACGGTCGCGCAGATCCCAAGGAAGTCTCAGGCTTGGTGATCCACAACTGCAGAATCGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGTTGGATACATGCCCTGGTCTGGAAATTTTGCACTGGAGACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGTGCTGTCACCAATAGGAGAGTGAGATGGAAGGGTGTTAAAGTGATTGGAAGAAATGAAGCCATGCAGTTCACTGCTGGACCTTTTCTTCAAGGAAGAGCTTGGCTTCCAACCACTGGTGGCCCCTATCTATTGGGATTGAAAAGCTAA

Protein sequence

MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQNTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLLGLKS
Homology
BLAST of IVF0000713 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-125
Identity = 223/564 (39.54%), Postives = 350/564 (62.06%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQNTL 63
           + ++  IS +L++ + +AV   V  NKS +A  E+++  +KA+  +CA  +Y++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E V+ A  +T++++      +   MIE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 SDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL         +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIY EYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGPFLQGRAWLPTTGGPYLLGLKS 568
              ++QG AW+P  G PY+LGL S
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of IVF0000713 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.2e-124
Identity = 259/606 (42.74%), Postives = 363/606 (59.90%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           M+ KV++   S++L+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC + + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA---NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIE-----AANNRSIKMGVE 120
            TL        S+DP + ++A +L+T +E+ K  N T    +      + NN+++   ++
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L
Sbjct: 121 YCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--L 180

Query: 181 KQKMQDGLSDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           ++ + +G++++  LT+NA+ I   V   +A    ++                        
Sbjct: 181 RKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPV 240

Query: 241 AQPSG--RRLLGTTEVGKDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDG 300
           A P G  RRLL   ++ + G PTW++GADRKL+A         RGGGA+V+ N VVAKDG
Sbjct: 241 ADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDG 300

Query: 301 SGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNK 360
           SGQFKT+  A+ A P+  RGR +IY+KAG+Y E V I K   NIFM+GDG +KT+++ N+
Sbjct: 301 SGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNR 360

Query: 361 S--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDG 420
           S     G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DG
Sbjct: 361 SVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDG 420

Query: 421 YQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADP 480
           YQDTLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G    
Sbjct: 421 YQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELG 480

Query: 481 KEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW 540
             +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W
Sbjct: 481 LGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIW 540

Query: 541 SGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGG 566
            G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA  +L    W+     
Sbjct: 541 DGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANV 588

BLAST of IVF0000713 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 2.1e-124
Identity = 251/599 (41.90%), Postives = 359/599 (59.93%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVH--------------SNKSSDAATEDLSPKM------ 63
           K I+ G+  +LVV  A+   A  +              +NKS D+ T+  SP        
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSTICANANYQQECQNTLSN-AAHNASSDDPKEYVRAAILSTIEEVKK 123
                     K + T+C +  Y+  CQNTL N    +    DP+  +++AI++  +++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKN 183
            +        E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDILASF----- 243
           WL++V+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L+S      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 GLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFK 303
            L L+A+ S +          D   +WL+  +R++L +    A +KPNA VAKDGSG F 
Sbjct: 263 RLLLEARSSAKE--------TDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFT 322

Query: 304 TISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGG 363
           TI+AAL A P   +GRY IY+K GIY+E V I K   N+ M GDG +KTIVTGNKS+   
Sbjct: 323 TINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKK 382

Query: 364 FTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYV 423
             T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY 
Sbjct: 383 IRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYA 442

Query: 424 QTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLV 483
            T RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V
Sbjct: 443 YTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFV 502

Query: 484 IHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW-SGNFALE 543
           IHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++
Sbjct: 503 IHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAID 562

Query: 544 TCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLLGL 566
           T  Y EY N GP   T  RV+W G +V+ + EAM+FT GPFLQG  W+   G P  LGL
Sbjct: 563 TLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of IVF0000713 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 445.7 bits (1145), Expect = 8.0e-124
Identity = 235/542 (43.36%), Postives = 345/542 (63.65%), Query Frame = 0

Query: 28  SNKSSDAATEDLSPKM--KAVSTICANANYQQECQNTLSNAAHNA-SSDDPKEYVRAAIL 87
           S K+  +A + + P    K + T+C++  Y Q C+ TL N      + D+P  ++++AI 
Sbjct: 89  SQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIE 148

Query: 88  STIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNA 147
           +  E++     + +  +     N+  K  +E C+ L++ A ++  AS + +   ++++  
Sbjct: 149 AVNEDLDL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFE 208

Query: 148 DRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDIL 207
             V D+++WL++V+SYQ++CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L
Sbjct: 209 KVVPDLESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENL 268

Query: 208 ASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSG 267
           +     ++     R LL       D  P+W++  DR++L +    A +KPNA VAKDGSG
Sbjct: 269 SPVMKVVE-----RHLL-------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSG 328

Query: 268 QFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSN 327
            F TI+ AL A P+   GRY+IYVK GIY+EYV + K   N+ M GDG +KTIVTGNKS+
Sbjct: 329 DFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSH 388

Query: 328 RGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 387
                T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDT
Sbjct: 389 AKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDT 448

Query: 388 LYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS 447
           LY  T RQ+YR+CVI GT+DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +
Sbjct: 449 LYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTT 508

Query: 448 GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW-SGNF 507
           G V+HNC+I   + L   + +  ++LGRPWK Y+RT+IMES + + I PVG++ W   +F
Sbjct: 509 GFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDF 568

Query: 508 ALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLL 566
           A++T  Y EY N+G    T  RV+W G KVI + EA+ +T GPFLQG  W+  +G P  L
Sbjct: 569 AIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKL 611

BLAST of IVF0000713 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 444.5 bits (1142), Expect = 1.8e-123
Identity = 221/564 (39.18%), Postives = 344/564 (60.99%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVHSNK--SSDAATEDLSPKMKAVSTICANANYQQECQN 63
           + I+  IS +L++ + +AV   V  NK         +++  +KAV  +CA  +Y++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLL 123
           TL     N +  DP E V+ A   T++++      +   M E   +   +M ++ C++L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQD 183
            +A+D+L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 GLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRK 243
            L  A +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RK
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRK 253

Query: 244 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITK 303
           LL +    + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+Y EYV + K
Sbjct: 254 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 313

Query: 304 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 363
            M ++   GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQA
Sbjct: 314 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 373

Query: 364 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 423
           VA+RVQSD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++V
Sbjct: 374 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 433

Query: 424 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 483
           R+P+ NQ   +TA+GR DP+E +G V   C I  E    A +     +LGRPWK+Y+RT+
Sbjct: 434 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 493

Query: 484 IMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 543
           IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++
Sbjct: 494 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 553

Query: 544 FTAGPFLQGRAWLPTTGGPYLLGL 566
           FT   ++QG  W+P  G PY  GL
Sbjct: 554 FTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of IVF0000713 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 568
           QFTAGPFLQGRAWLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of IVF0000713 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 566/567 (99.82%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MVNKVILPGI+LILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 568
           QFTAGPFLQGRAWLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of IVF0000713 vs. ExPASy TrEMBL
Match: A0A5A7U7W4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.6e-276
Identity = 473/567 (83.42%), Postives = 513/567 (90.48%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MV KV++  ++ +LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ
Sbjct: 1   MVAKVVM-AVTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL N  HN+S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
            GL  A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 568
           QFTAGPFL GRAWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of IVF0000713 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.6e-276
Identity = 472/567 (83.25%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MV KV++ GISLILVVGVALAVVAIV+ + SSD  T +LSPKMKAV++IC+NA+YQ ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSD-ETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL +  +N+S +DPKE+++AAI STIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGL  AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEV  D +PTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+YNE + +T
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 568
           QFTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of IVF0000713 vs. ExPASy TrEMBL
Match: A0A1S3CR40 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 3.0e-275
Identity = 468/558 (83.87%), Postives = 507/558 (90.86%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQNTLSNAAHN 69
           ++ +LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ TL N  HN
Sbjct: 3   VTSLLVVGVVLAVFAMVNKNHSSD-ETADMSPKMKAVSSICSNTDYQQECQTTLGNVGHN 62

Query: 70  ASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQ 129
           +S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDLLQ AID+L 
Sbjct: 63  SSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLLQSAIDELH 122

Query: 130 ASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKL 189
           ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ GL  A KL
Sbjct: 123 ASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQGGLDVAAKL 182

Query: 190 TSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGG 249
           TSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DG+PTWLTGADRKLLA++ GG
Sbjct: 183 TSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRKLLAAKRGG 242

Query: 250 AKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMY 309
            +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +TKDMKN+FMY
Sbjct: 243 VRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTKDMKNVFMY 302

Query: 310 GDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSD 369
           GDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQAVALRVQSD
Sbjct: 303 GDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSD 362

Query: 370 RSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQ 429
           RSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLIIVRRPMDNQQ
Sbjct: 363 RSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIVRRPMDNQQ 422

Query: 430 NTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGD 489
           NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YARTVIME+TLGD
Sbjct: 423 NTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMETTLGD 482

Query: 490 FIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQ 549
           FIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAMQFTAGPFL 
Sbjct: 483 FIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQFTAGPFLL 542

Query: 550 GRAWLPTTGGPYLLGLKS 568
           GRAWLP TGGPYLLGLK+
Sbjct: 543 GRAWLPATGGPYLLGLKN 559

BLAST of IVF0000713 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1127 bits (2916), Expect = 0.0
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567
           QFTAGPFLQGRAWLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of IVF0000713 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 566/567 (99.82%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MVNKVILPGI+LILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567
           QFTAGPFLQGRAWLPTTGGPYLLGLKS
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of IVF0000713 vs. NCBI nr
Match: XP_038875878.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 498/567 (87.83%), Postives = 534/567 (94.18%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MVNKV+LPGISLILVVGVALAVVA+V  NK++++ TE+LSPKMKAVSTIC+N NYQ+ECQ
Sbjct: 1   MVNKVVLPGISLILVVGVALAVVAVV--NKNNNSTTENLSPKMKAVSTICSNTNYQEECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
           NTL+N AHNASSDDPKEYV+AAIL+T+EEVKKGYNLTDGFMIEAANN SIKMGVEDC+DL
Sbjct: 61  NTLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAI QLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGL EFDP L+QKMQ
Sbjct: 121 LQFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
           DGL  A KLTSNALAIVD VSDIL +FGLQ K QPSGR+LLGT EVG DG+PTW++GADR
Sbjct: 181 DGLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLASRGGG +VKPNAVVAKDGSGQFKTI AALAAYPKAL+GRYVIYVKAGIYNEYV +T
Sbjct: 241 KLLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDM NIFMYGDGPK+TIVTG +SNR G+TTQDTATFIAIG GFLCKSMGFQNTAGPEGHQ
Sbjct: 301 KDMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI R EA+
Sbjct: 481 VIMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEAL 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567
           +FTAG FLQG +WLP TGGPYLLGLK 
Sbjct: 541 KFTAGSFLQGGSWLPGTGGPYLLGLKG 564

BLAST of IVF0000713 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 478/567 (84.30%), Postives = 521/567 (91.89%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLS-PKMKAVSTICANANYQQEC 60
           MV KV++ GISLILVVGVALAVVA+V  N +SD    DLS PKMKAVSTIC+  +YQQEC
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDV---DLSSPKMKAVSTICSTTDYQQEC 60

Query: 61  QNTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRD 120
           QNTL+N A NAS DDPKEY +AA+L+TI+E+ KGYNLT   M+EAANN SIKM V+DC+D
Sbjct: 61  QNTLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKD 120

Query: 121 LLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKM 180
           LLQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKM
Sbjct: 121 LLQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180

Query: 181 QDGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGAD 240
           QDGL  AGKLTSNALAIV AVSDIL +FGLQLK QPSGRRLL TTE+G DG+PTW++GAD
Sbjct: 181 QDGLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGAD 240

Query: 241 RKLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHI 300
           RKLLASRGGGA+VKPNAVVAKDGSGQ+K+I AALAAYPKALRGRYVIYVKAGIY+EY+ +
Sbjct: 241 RKLLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITV 300

Query: 301 TKDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGH 360
           TKDMKN+FMYGDGP+KTIVTG K+NR G TTQ+TA+F AIGEGFLCKSMGF+NTAGPEGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 420
           QAVALRVQSDRSAF+NCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLI
Sbjct: 361 QAVALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420

Query: 421 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480
           IVRRPMDNQ+NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR
Sbjct: 421 IVRRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEA 540
           TVIME+TLGDFIQP GYMPWSGNFALETC Y EYGNRGPGA TNRRVRWKGV+VIGRNEA
Sbjct: 481 TVIMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 MQFTAGPFLQGRAWLPTTGGPYLLGLK 566
           +Q+TAGPFL GRAWLP TGGPYLLGLK
Sbjct: 541 LQYTAGPFLMGRAWLPATGGPYLLGLK 564

BLAST of IVF0000713 vs. NCBI nr
Match: KAA0050276.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 475/567 (83.77%), Postives = 512/567 (90.30%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           MV KV++   SL LVVGV LAV A+V+ N SSD  T D+SPKMKAVS+IC+N +YQQECQ
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNKNHSSDE-TADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120
            TL N  HN+S DDPKE+++AAI +TIEE+KKGYNLTD  M+EAANN SIKM V+DCRDL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240
            GL  A KLTSNALAIVDAVSDIL S GLQLK QPSGRRLLGTTEV  DGYPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300
           KLLA++ GG +VKPNAVVAKDGSGQ+KTI+AALAAYPKAL+GRYVIYVKAGIYNEY+ +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR G  TQ+TATF AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCV+SGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540
           VIME+TLGDFIQP GYMPWSG+FAL TC Y EYGNRGPGA T RRVRWKGV+VIGRNEAM
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567
           QFTAGPFL GRAWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of IVF0000713 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 451.8 bits (1161), Expect = 8.0e-127
Identity = 223/564 (39.54%), Postives = 350/564 (62.06%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQNTL 63
           + ++  IS +L++ + +AV   V  NKS +A  E+++  +KA+  +CA  +Y++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQF 123
              A + S  DP E V+ A  +T++++      +   MIE   +   KM ++ C++L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQ-TMIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGL 183
           AI +L  S+  +G+ + H   + +  ++ WL++ IS++Q+CLDG +       + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 SDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLL 243
             A +LT N LA+V  +S+ L    +    + + RRLL         +P+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQI---PEMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDM 303
                 ++VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++K GIY EYV + + M
Sbjct: 255 --NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVA 363
            ++   GDGP KT+++G+KS + G TT  TAT   +G+ F+ K++ F+NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRR 423
           +RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E ++FT
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGPFLQGRAWLPTTGGPYLLGLKS 568
              ++QG AW+P  G PY+LGL S
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGLFS 564

BLAST of IVF0000713 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.4 bits (1152), Expect = 8.8e-126
Identity = 259/606 (42.74%), Postives = 363/606 (59.90%), Query Frame = 0

Query: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60
           M+ KV++   S++L+VGVA+ VVA +  NK+ DA   +LSP+MKAV  IC + + +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFI--NKNGDA---NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIE-----AANNRSIKMGVE 120
            TL        S+DP + ++A +L+T +E+ K  N T    +      + NN+++   ++
Sbjct: 61  KTL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAV---LD 120

Query: 121 DCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQL 180
            C+ +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L
Sbjct: 121 YCKRVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--L 180

Query: 181 KQKMQDGLSDAGKLTSNALAIVDAVSDILASFGLQLK----------------------- 240
           ++ + +G++++  LT+NA+ I   V   +A    ++                        
Sbjct: 181 RKAIGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPV 240

Query: 241 AQPSG--RRLLGTTEVGKDGYPTWLTGADRKLLA--------SRGGGAKVKPNAVVAKDG 300
           A P G  RRLL   ++ + G PTW++GADRKL+A         RGGGA+V+ N VVAKDG
Sbjct: 241 ADPDGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDG 300

Query: 301 SGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNK 360
           SGQFKT+  A+ A P+  RGR +IY+KAG+Y E V I K   NIFM+GDG +KT+++ N+
Sbjct: 301 SGQFKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNR 360

Query: 361 S--NRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDG 420
           S     G TT  +AT     EGF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DG
Sbjct: 361 SVALSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDG 420

Query: 421 YQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADP 480
           YQDTLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G    
Sbjct: 421 YQDTLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELG 480

Query: 481 KEVS-GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW 540
             +  G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W
Sbjct: 481 LGMKIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIW 540

Query: 541 SGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGG 566
            G    ++C Y+EY NRGPGA  NRRV W  V      E   FTA  +L    W+     
Sbjct: 541 DGESFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANV 588

BLAST of IVF0000713 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 447.6 bits (1150), Expect = 1.5e-125
Identity = 251/599 (41.90%), Postives = 359/599 (59.93%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVH--------------SNKSSDAATEDLSPKM------ 63
           K I+ G+  +LVV  A+   A  +              +NKS D+ T+  SP        
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSTICANANYQQECQNTLSN-AAHNASSDDPKEYVRAAILSTIEEVKK 123
                     K + T+C +  Y+  CQNTL N    +    DP+  +++AI++  +++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNADRVADIKN 183
            +        E   N+  K  +  C+ L+  A ++L  S   + + +++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDILASF----- 243
           WL++V+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L+S      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 GLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSGQFK 303
            L L+A+ S +          D   +WL+  +R++L +    A +KPNA VAKDGSG F 
Sbjct: 263 RLLLEARSSAKE--------TDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFT 322

Query: 304 TISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSNRGG 363
           TI+AAL A P   +GRY IY+K GIY+E V I K   N+ M GDG +KTIVTGNKS+   
Sbjct: 323 TINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKK 382

Query: 364 FTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYV 423
             T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY 
Sbjct: 383 IRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYA 442

Query: 424 QTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLV 483
            T RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V
Sbjct: 443 YTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFV 502

Query: 484 IHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW-SGNFALE 543
           IHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I PVG++ W   +FA++
Sbjct: 503 IHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAID 562

Query: 544 TCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLLGL 566
           T  Y EY N GP   T  RV+W G +V+ + EAM+FT GPFLQG  W+   G P  LGL
Sbjct: 563 TLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of IVF0000713 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 445.7 bits (1145), Expect = 5.7e-125
Identity = 235/542 (43.36%), Postives = 345/542 (63.65%), Query Frame = 0

Query: 28  SNKSSDAATEDLSPKM--KAVSTICANANYQQECQNTLSNAAHNA-SSDDPKEYVRAAIL 87
           S K+  +A + + P    K + T+C++  Y Q C+ TL N      + D+P  ++++AI 
Sbjct: 89  SQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIE 148

Query: 88  STIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLLQFAIDQLQASYSTVGEPDLHTNA 147
           +  E++     + +  +     N+  K  +E C+ L++ A ++  AS + +   ++++  
Sbjct: 149 AVNEDLDL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFE 208

Query: 148 DRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQDGLSDAGKLTSNALAIVDAVSDIL 207
             V D+++WL++V+SYQ++CLDG EE    LK +++  ++ +  LTSN+LA++   ++ L
Sbjct: 209 KVVPDLESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENL 268

Query: 208 ASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRKLLASRGGGAKVKPNAVVAKDGSG 267
           +     ++     R LL       D  P+W++  DR++L +    A +KPNA VAKDGSG
Sbjct: 269 SPVMKVVE-----RHLL-------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSG 328

Query: 268 QFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITKDMKNIFMYGDGPKKTIVTGNKSN 327
            F TI+ AL A P+   GRY+IYVK GIY+EYV + K   N+ M GDG +KTIVTGNKS+
Sbjct: 329 DFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSH 388

Query: 328 RGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 387
                T  TATF+A GEGF+ +SMGF+NTAGPEGHQAVA+RVQSDRS F NCR +GYQDT
Sbjct: 389 AKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDT 448

Query: 388 LYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS 447
           LY  T RQ+YR+CVI GT+DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +
Sbjct: 449 LYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTT 508

Query: 448 GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPVGYMPW-SGNF 507
           G V+HNC+I   + L   + +  ++LGRPWK Y+RT+IMES + + I PVG++ W   +F
Sbjct: 509 GFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDF 568

Query: 508 ALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQFTAGPFLQGRAWLPTTGGPYLL 566
           A++T  Y EY N+G    T  RV+W G KVI + EA+ +T GPFLQG  W+  +G P  L
Sbjct: 569 AIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKL 611

BLAST of IVF0000713 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-124
Identity = 221/564 (39.18%), Postives = 344/564 (60.99%), Query Frame = 0

Query: 4   KVILPGISLILVVGVALAVVAIVHSNK--SSDAATEDLSPKMKAVSTICANANYQQECQN 63
           + I+  IS +L++ + +AV   V  NK         +++  +KAV  +CA  +Y++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDLL 123
           TL     N +  DP E V+ A   T++++      +   M E   +   +M ++ C++L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQD 183
            +A+D+L  S+  +G+ + H   + + +++ WL++ IS++++CL+G +       + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 GLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADRK 243
            L  A +LT N LAI+  +S+ +    +Q+    S RRLL       +G+P+W+    RK
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQ--MQIPGLNS-RRLLA------EGFPSWVDQRGRK 253

Query: 244 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHITK 303
           LL +    + VKP+ VVA+DGSGQ+KTI+ AL   PK     +V+++KAG+Y EYV + K
Sbjct: 254 LLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNK 313

Query: 304 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 363
            M ++   GDGP KTI++GNK+ + G TT  TAT   +G  F+ K++GF+NTAG   HQA
Sbjct: 314 TMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQA 373

Query: 364 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 423
           VA+RVQSD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++V
Sbjct: 374 VAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLV 433

Query: 424 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 483
           R+P+ NQ   +TA+GR DP+E +G V   C I  E    A +     +LGRPWK+Y+RT+
Sbjct: 434 RKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTI 493

Query: 484 IMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 543
           IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + ++
Sbjct: 494 IMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILK 553

Query: 544 FTAGPFLQGRAWLPTTGGPYLLGL 566
           FT   ++QG  W+P  G PY  GL
Sbjct: 554 FTPAQYIQGDDWIPGKGVPYTTGL 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E8Z81.1e-12539.54Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
O807221.2e-12442.74Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q9SMY62.1e-12441.90Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Q7Y2018.0e-12443.36Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q8GX861.8e-12339.18Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7U9J80.0e+00100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR270.0e+0099.82Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A5A7U7W43.6e-27683.42Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00090... [more]
A0A0A0KLN23.6e-27683.25Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A1S3CR403.0e-27583.87Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503798 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAA0050275.10.0100.00pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.10.099.82PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875878.10.087.83pectinesterase-like [Benincasa hispida][more]
XP_038875879.10.084.30LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KAA0050276.10.083.77pectinesterase-like [Cucumis melo var. makuwa] >TYJ98131.1 pectinesterase-like [... [more]
Match NameE-valueIdentityDescription
AT5G27870.18.0e-12739.54Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.18.8e-12642.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.5e-12541.90Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.15.7e-12543.36Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.11.3e-12439.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..196
e-value: 4.5E-32
score: 122.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 22..198
e-value: 2.8E-17
score: 60.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..196
e-value: 3.6E-23
score: 82.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 43..201
e-value: 1.9E-28
score: 101.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 44..200
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 255..550
e-value: 3.2E-130
score: 433.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 249..565
e-value: 2.4E-110
score: 370.9
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 4..565
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..565
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 48..201
e-value: 6.74735E-46
score: 156.83
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 399..408
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 252..564

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000713.2IVF0000713.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity