IVF0000691 (gene) Melon (IVF77) v1

Overview
NameIVF0000691
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionaspartic proteinase CDR1
Locationchr11: 23186194 .. 23189431 (-)
RNA-Seq ExpressionIVF0000691
SyntenyIVF0000691
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTAACATTACACTCTCTGTTTTGTATGTTAGGTTACAGGAAGCCAATGTCGCCTATTTCAAATTTTTGTTTCTTCTTCCTCCTCCTCTTCTTCTTATCCTTCTCCTCCTCCTTCCTCTTTGCATTGGGCGACGAAGCCAACAACTACAACAACAATGACGACGAAGACGAACAACAAACAATCAGATTTGATCTACTTCACCGTCACCATCCACAAGTCTCCGAAAAGCTTAACGGTGATATGAAAATCCAAGATCTTCATGAAAGAATGAAAGACATTCATGAACACGACCGCAATCGTCACCGCTCCATCTCCAAATCCATGAACCAGAAGCAAATTGAGGATGCGAGGTTGAGGGCAGAGGCAGAGGCGGCGACACAGGTAGAGGTGGCAAAGAGTGCAATACTTCCACCGGCAACGTCGACGCCGATAGGAATGAAAATGATTTCGGGTGCAGATTTTGGGAGTAGTGAGTATTTCGTACAATTGAAGGTGGGAACACCGGCGCAAACGTTTATGTTGATTGCGGATACAGGGAGCGATTTAACGTGGTTGAAATGTAGATATCGAAGGTGTTTTGGGAATTGTAGCGGTAACGTGAATCATAAAAGCAAAAATGAAAAGAAACAGAGATTTAGACATGCGTTATTGGCGAATCAGTCGTCTACTTTTAAGACAGTTTCTTGCAGCTCAACGATGTGTACAAATAATCTTGCGGAATTGTTTGCTGTTGCGGAATGCGACACCCCAACTAGTCCTTGTGTCTATGATTACAGGTAGGTACGTACATGCCTACACTCTGTATTTTCCTAATTAATTCTATAAAAAATAAAAAACCATAATCAAATAAATAAAAAGTTTGGGGTATTTTAAAAAATATAAAAAAACGAGAAAGTATTTGTTTGATTTGTTACACGATCGTTCATTTGATACGATCGTTTGATACGGTCGTTTGATTTGTTACACGATCCGTTTGATACGATTGTTTGATTTGTTACACGATCCGTTTGATACGATGGTTTGATTTGTTACACGATCCGTTTGATACGATCGTTTGATTTCTTACACGATCTGTTGGACACGATCGTTTGATTTCTTACACGATCTGTTGGACACGATCGTTTGATTTCTTACACGATCTGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCATTTGATGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATGATCGTTTGATTTCTTACACGATCCGTTGGACACGATCGTTTGATGATCGTTTGATTTCTTACACGATCGTTCGTTTGATACGATCGTTTGATTTGTTACACGATCGTTCGTTTGATACGTTCGTTTGATTTGTTACACGATCGTTCGTTTGATACGTTCGTTTGATTTGTTACACGATCGTTCGTTCGTTTGATACGATCGTTTGATTTGTTACACGATCGTTCGTTCGTTTGATACGATTGTTTGATTCGTTACACGATCGTTTGATTCGTTGACACCATCGTTTGATTCGTTTACAAGATCGTTTGTGATTTAGAATCCTAAATCTAAACGACTTTTTTTTCAAAATTTGGTATAGATTTGGATATTCTAATCTAAACTATTTTTTAATTTTTTTTTTTCAAAATAGGTATACTATCCTTACATGCACGTAAGAAGGGAAAAACAAAAAATAGAAAAATTGCAAAAAAATATGAAAATATGAAATATTTAAAATTGACTAAATCTATTAGTTTTGTTACCATTTGTATATTTAGGAAAGCTTCATAAAAAGTTTAATTTTTTTATTTTTGAATTCTTTTAAAAGGGATATAATTTTGGTTTCTTTGGTATTAAAAACAGCTACGCAGGAGGTGCAAGTGCAAAGGGAATATTCGCATGGGAGACTCTAACTGTAGGCTTAACAAACGGAAAAGAAAAACAACTCCGTAATTCCATAATAGGATGTACGGAAATCGTCCAAGGCAACGTTTTCGATGGAGCCGACGGTGTCATGGGCTTAGGCACTAGCTCCTATTCTTTAACTTACAAAGCCGCAGAAAACGCCAACGGCGGCGGCTTCTCTTACTGCCTTGTCGATCATCTCACCGATCAAAGAGCTGTCAGTTATTTCGTCCTCGGCGTTCCTACCCCTTCCACTTCCGCCTCCACTTCCTCTGCTAAACCTCCCGCCAAAATGTCCTACACCAAACTCTACGTTGGCGACCCTTACAGCAGCTTCTACGGCGTCGATCTCATCGGTATCTCCGCCGACGGCCAAATGCTCAACATCCCTCCTCGTGTTTGGGACAGCTATAAAGGCTGCGGTACCATCATCGATTCCGGTACTAGTCTCACTGTGCTTGCCACTCCTGCTTTCGATGTGGTAATGGAAGTTTTGACTTCGAGATTGAAGCAATTTCAGCAAATTGAGATCGAACCTTTCAATTTTTGCTTCAATAATAGCCAATACACTCACGACATGGCGCCGAAGCTCCGATTCCATTTCGGTGACGGAACGGTGTTTGAGCCGCCGACCAAAAGCTACATTGTGTCGGTGGGGGAATTCATTAGTTGTATTGGAATCGTTTCGATGCCTTTTCCGTCCCTCAATATCATTGGAAATATTCTTCAGCAAAATCACCTTTGGCAATTTGATTTCCAAAAGAGAAGAGTCGGTTTTGCCGCTTCCGAATGCATCTAAAAGAAATAATAATAATACAAACTTCATCATAATTAATTATTTTCATTTTCAATTTTTATTATAATTTTTTGTTTCTATTATTAACTAATACATCTATCTTATTATTGAAATATATACCATCTCAAATTTCTCTTTAATTTTCCTTTTCTATACATACACAATATATGTCTTTTCTTTTTATTTTTTCCTTTTTCATTTTGGAATAACTTGGAGAGTGGAGATTTTTTTTTAATTTTCTTTCTTGTGTAAAGAGTTGAAGAAGAAGATGATTGTATCTTGAATAATATTTACTAGAAAAGATAGAAAGAAGAGATATTCTTTGGTTCCTC

mRNA sequence

CTCTAACATTACACTCTCTGTTTTGTATGTTAGGTTACAGGAAGCCAATGTCGCCTATTTCAAATTTTTGTTTCTTCTTCCTCCTCCTCTTCTTCTTATCCTTCTCCTCCTCCTTCCTCTTTGCATTGGGCGACGAAGCCAACAACTACAACAACAATGACGACGAAGACGAACAACAAACAATCAGATTTGATCTACTTCACCGTCACCATCCACAAGTCTCCGAAAAGCTTAACGGTGATATGAAAATCCAAGATCTTCATGAAAGAATGAAAGACATTCATGAACACGACCGCAATCGTCACCGCTCCATCTCCAAATCCATGAACCAGAAGCAAATTGAGGATGCGAGGTTGAGGGCAGAGGCAGAGGCGGCGACACAGGTAGAGGTGGCAAAGAGTGCAATACTTCCACCGGCAACGTCGACGCCGATAGGAATGAAAATGATTTCGGGTGCAGATTTTGGGAGTAGTGAGTATTTCGTACAATTGAAGGTGGGAACACCGGCGCAAACGTTTATGTTGATTGCGGATACAGGGAGCGATTTAACGTGGTTGAAATGTAGATATCGAAGGTGTTTTGGGAATTGTAGCGGTAACGTGAATCATAAAAGCAAAAATGAAAAGAAACAGAGATTTAGACATGCGTTATTGGCGAATCAGTCGTCTACTTTTAAGACAGTTTCTTGCAGCTCAACGATGTGTACAAATAATCTTGCGGAATTGTTTGCTGTTGCGGAATGCGACACCCCAACTAGTCCTTGTGTCTATGATTACAGCTACGCAGGAGGTGCAAGTGCAAAGGGAATATTCGCATGGGAGACTCTAACTGTAGGCTTAACAAACGGAAAAGAAAAACAACTCCGTAATTCCATAATAGGATGTACGGAAATCGTCCAAGGCAACGTTTTCGATGGAGCCGACGGTGTCATGGGCTTAGGCACTAGCTCCTATTCTTTAACTTACAAAGCCGCAGAAAACGCCAACGGCGGCGGCTTCTCTTACTGCCTTGTCGATCATCTCACCGATCAAAGAGCTGTCAGTTATTTCGTCCTCGGCGTTCCTACCCCTTCCACTTCCGCCTCCACTTCCTCTGCTAAACCTCCCGCCAAAATGTCCTACACCAAACTCTACGTTGGCGACCCTTACAGCAGCTTCTACGGCGTCGATCTCATCGGTATCTCCGCCGACGGCCAAATGCTCAACATCCCTCCTCGTGTTTGGGACAGCTATAAAGGCTGCGGTACCATCATCGATTCCGGTACTAGTCTCACTGTGCTTGCCACTCCTGCTTTCGATGTGGTAATGGAAGTTTTGACTTCGAGATTGAAGCAATTTCAGCAAATTGAGATCGAACCTTTCAATTTTTGCTTCAATAATAGCCAATACACTCACGACATGGCGCCGAAGCTCCGATTCCATTTCGGTGACGGAACGGTGTTTGAGCCGCCGACCAAAAGCTACATTGTGTCGGTGGGGGAATTCATTAGTTGTATTGGAATCGTTTCGATGCCTTTTCCGTCCCTCAATATCATTGGAAATATTCTTCAGCAAAATCACCTTTGGCAATTTGATTTCCAAAAGAGAAGAGTCGGTTTTGCCGCTTCCGAATGCATCTAAAAGAAATAATAATAATACAAACTTCATCATAATTAATTATTTTCATTTTCAATTTTTATTATAATTTTTTGTTTCTATTATTAACTAATACATCTATCTTATTATTGAAATATATACCATCTCAAATTTCTCTTTAATTTTCCTTTTCTATACATACACAATATATGTCTTTTCTTTTTATTTTTTCCTTTTTCATTTTGGAATAACTTGGAGAGTGGAGATTTTTTTTTAATTTTCTTTCTTGTGTAAAGAGTTGAAGAAGAAGATGATTGTATCTTGAATAATATTTACTAGAAAAGATAGAAAGAAGAGATATTCTTTGGTTCCTC

Coding sequence (CDS)

ATGTTAGGTTACAGGAAGCCAATGTCGCCTATTTCAAATTTTTGTTTCTTCTTCCTCCTCCTCTTCTTCTTATCCTTCTCCTCCTCCTTCCTCTTTGCATTGGGCGACGAAGCCAACAACTACAACAACAATGACGACGAAGACGAACAACAAACAATCAGATTTGATCTACTTCACCGTCACCATCCACAAGTCTCCGAAAAGCTTAACGGTGATATGAAAATCCAAGATCTTCATGAAAGAATGAAAGACATTCATGAACACGACCGCAATCGTCACCGCTCCATCTCCAAATCCATGAACCAGAAGCAAATTGAGGATGCGAGGTTGAGGGCAGAGGCAGAGGCGGCGACACAGGTAGAGGTGGCAAAGAGTGCAATACTTCCACCGGCAACGTCGACGCCGATAGGAATGAAAATGATTTCGGGTGCAGATTTTGGGAGTAGTGAGTATTTCGTACAATTGAAGGTGGGAACACCGGCGCAAACGTTTATGTTGATTGCGGATACAGGGAGCGATTTAACGTGGTTGAAATGTAGATATCGAAGGTGTTTTGGGAATTGTAGCGGTAACGTGAATCATAAAAGCAAAAATGAAAAGAAACAGAGATTTAGACATGCGTTATTGGCGAATCAGTCGTCTACTTTTAAGACAGTTTCTTGCAGCTCAACGATGTGTACAAATAATCTTGCGGAATTGTTTGCTGTTGCGGAATGCGACACCCCAACTAGTCCTTGTGTCTATGATTACAGCTACGCAGGAGGTGCAAGTGCAAAGGGAATATTCGCATGGGAGACTCTAACTGTAGGCTTAACAAACGGAAAAGAAAAACAACTCCGTAATTCCATAATAGGATGTACGGAAATCGTCCAAGGCAACGTTTTCGATGGAGCCGACGGTGTCATGGGCTTAGGCACTAGCTCCTATTCTTTAACTTACAAAGCCGCAGAAAACGCCAACGGCGGCGGCTTCTCTTACTGCCTTGTCGATCATCTCACCGATCAAAGAGCTGTCAGTTATTTCGTCCTCGGCGTTCCTACCCCTTCCACTTCCGCCTCCACTTCCTCTGCTAAACCTCCCGCCAAAATGTCCTACACCAAACTCTACGTTGGCGACCCTTACAGCAGCTTCTACGGCGTCGATCTCATCGGTATCTCCGCCGACGGCCAAATGCTCAACATCCCTCCTCGTGTTTGGGACAGCTATAAAGGCTGCGGTACCATCATCGATTCCGGTACTAGTCTCACTGTGCTTGCCACTCCTGCTTTCGATGTGGTAATGGAAGTTTTGACTTCGAGATTGAAGCAATTTCAGCAAATTGAGATCGAACCTTTCAATTTTTGCTTCAATAATAGCCAATACACTCACGACATGGCGCCGAAGCTCCGATTCCATTTCGGTGACGGAACGGTGTTTGAGCCGCCGACCAAAAGCTACATTGTGTCGGTGGGGGAATTCATTAGTTGTATTGGAATCGTTTCGATGCCTTTTCCGTCCCTCAATATCATTGGAAATATTCTTCAGCAAAATCACCTTTGGCAATTTGATTTCCAAAAGAGAAGAGTCGGTTTTGCCGCTTCCGAATGCATCTAA

Protein sequence

MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI
Homology
BLAST of IVF0000691 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.7e-81
Identity = 171/455 (37.58%), Postives = 258/455 (56.70%), Query Frame = 0

Query: 80  ERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATSTPIGMK 139
           + MKD     +  HR         +IED             +  + +++    ++ +G+K
Sbjct: 42  DSMKDTSVRLKLAHRDTLLPKPLSRIED---------VIGADQKRHSLISRKRNSTVGVK 101

Query: 140 MI--SGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVNHKSK 199
           M   SG D+G+++YF +++VGTPA+ F ++ DTGS+LTW+ CRYR       G  N    
Sbjct: 102 MDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRYR-----ARGKDN---- 161

Query: 200 NEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYAGGAS 259
                  R    A++S +FKTV C +  C  +L  LF++  C TP++PC YDY YA G++
Sbjct: 162 -------RRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSA 221

Query: 260 AKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAE 319
           A+G+FA ET+TVGLTNG+  +L   +IGC+    G  F GADGV+GL  S +S T   A 
Sbjct: 222 AQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFT-STAT 281

Query: 320 NANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDPYSSF 379
           +  G  FSYCLVDHL+++   +Y + G    S+ ++ ++ +    +  T++        F
Sbjct: 282 SLYGAKFSYCLVDHLSNKNVSNYLIFG----SSRSTKTAFRRTTPLDLTRI------PPF 341

Query: 380 YGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRLKQF 439
           Y +++IGIS    ML+IP +VWD+  G GTI+DSGTSLT+LA  A+  V+  L   L + 
Sbjct: 342 YAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVEL 401

Query: 440 QQIEIE--PFNFCFN-NSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVS 499
           ++++ E  P  +CF+  S +     P+L FH   G  FEP  KSY+V     + C+G VS
Sbjct: 402 KRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVS 460

Query: 500 MPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
              P+ N+IGNI+QQN+LW+FD     + FA S C
Sbjct: 462 AGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of IVF0000691 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.9e-35
Identity = 116/396 (29.29%), Postives = 187/396 (47.22%), Query Frame = 0

Query: 140 MISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR-YRRCFGNCSGNVNHKSKN 199
           ++SGA  GS EYF ++ VGTPA+   L+ DTGSD+ W++C     C+       N  S  
Sbjct: 151 VVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTS-- 210

Query: 200 EKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYAGGASA 259
                         SST+K+++CS+  C+     L   + C   ++ C+Y  SY  G+  
Sbjct: 211 --------------SSTYKSLTCSAPQCS-----LLETSAC--RSNKCLYQVSYGDGSFT 270

Query: 260 KGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAEN 319
            G  A +T+T G  +GK   + N  +GC    +G +F GA G++GLG    S+T      
Sbjct: 271 VGELATDTVTFG-NSGK---INNVALGCGHDNEG-LFTGAAGLLGLGGGVLSIT----NQ 330

Query: 320 ANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDPYSSFY 379
                FSYCLVD  + +             S+S   +S +     +   L       +FY
Sbjct: 331 MKATSFSYCLVDRDSGK-------------SSSLDFNSVQLGGGDATAPLLRNKKIDTFY 390

Query: 380 GVDLIGISADGQMLNIPPRVW--DSYKGCGTIIDSGTSLTVLATPAFDVVMEV---LTSR 439
            V L G S  G+ + +P  ++  D+    G I+D GT++T L T A++ + +    LT  
Sbjct: 391 YVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVN 450

Query: 440 LKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIV 499
           LK+     I  F+ C++ S  +    P + FHF  G   + P K+Y++ V +  +     
Sbjct: 451 LKKGSS-SISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAF 500

Query: 500 SMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
           +    SL+IIGN+ QQ     +D  K  +G + ++C
Sbjct: 511 APTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of IVF0000691 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-31
Identity = 117/406 (28.82%), Postives = 164/406 (40.39%), Query Frame = 0

Query: 129 PPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNC 188
           P  TS P+     SG       Y V+ K+GTP Q   ++ DT +D  WL C        C
Sbjct: 86  PKPTSVPVA----SGNQLHIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPC------SGC 145

Query: 189 SGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVY 248
           SG  N  +          +   N SST+ TVSCS+  CT   A            S C +
Sbjct: 146 SGCSNAST----------SFNTNSSSTYSTVSCSTAQCTQ--ARGLTCPSSSPQPSVCSF 205

Query: 249 DYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSS 308
           + SY G +S       +TLT+         + N   GC     GN      G+MGLG   
Sbjct: 206 NQSYGGDSSFSASLVQDTLTLA-----PDVIPNFSFGCINSASGNSLP-PQGLMGLGRGP 265

Query: 309 YSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKL 368
            SL  +   +   G FSYCL    +      YF       S S        P  + YT L
Sbjct: 266 MSLVSQTT-SLYSGVFSYCLPSFRS-----FYF-------SGSLKLGLLGQPKSIRYTPL 325

Query: 369 YVGDPYSSFYGVDLIGISADGQMLNIPP--RVWDSYKGCGTIIDSGTSLTVLATPAFDVV 428
                  S Y V+L G+S     + + P    +D+  G GTIIDSGT +T  A P ++ +
Sbjct: 326 LRNPRRPSLYYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAI 385

Query: 429 MEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEF 488
            +    ++       +  F+ CF  S    ++APK+  H     +  P   + I S    
Sbjct: 386 RDEFRKQVNVSSFSTLGAFDTCF--SADNENVAPKITLHMTSLDLKLPMENTLIHSSAGT 445

Query: 489 ISCI---GIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
           ++C+   GI       LN+I N+ QQN    FD    R+G A   C
Sbjct: 446 LTCLSMAGIRQNANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPC 448

BLAST of IVF0000691 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-31
Identity = 115/385 (29.87%), Postives = 172/385 (44.68%), Query Frame = 0

Query: 148 SSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR-YRRCFGNCSGNVNHKSKNEKKQRFRH 207
           S EY + + +GTP    M IADTGSDL W +C     C+       + K+          
Sbjct: 87  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKT---------- 146

Query: 208 ALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYAGGASAKGIFAWET 267
                 SST+K VSCSS+ CT     L   A C T  + C Y  SY   +  KG  A +T
Sbjct: 147 ------SSTYKDVSCSSSQCT----ALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 206

Query: 268 LTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAENANGGGFSY 327
           LT+G ++ +  QL+N IIGC     G       G++GLG    SL  +  ++ + G FSY
Sbjct: 207 LTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSID-GKFSY 266

Query: 328 CLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDPYSSFYGVDLIGIS 387
           CLV   + +   S    G     + +   S    AK S           +FY + L  IS
Sbjct: 267 CLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKAS---------QETFYYLTLKSIS 326

Query: 388 ADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRL-KQFQQIEIEPF 447
              + +       +S +G   IIDSGT+LT+L T  +  + + + S +  + +Q      
Sbjct: 327 VGSKQIQYSGSDSESSEG-NIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGL 386

Query: 448 NFCFNNSQYTHDM-APKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVSMPFPSLNIIG 507
           + C++    T D+  P +  HF DG   +  + +  V V E + C        PS +I G
Sbjct: 387 SLCYS---ATGDLKVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRGS--PSFSIYG 434

Query: 508 NILQQNHLWQFDFQKRRVGFAASEC 530
           N+ Q N L  +D   + V F  ++C
Sbjct: 447 NVAQMNFLVGYDTVSKTVSFKPTDC 434

BLAST of IVF0000691 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.8e-30
Identity = 152/542 (28.04%), Postives = 224/542 (41.33%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSF--LFALGDE---ANNYNNNDDEDEQQTIRF 60
           M+G RK +  + + CFFFL L   S   SF  LF        A+  +   D D +  +  
Sbjct: 1   MVGRRKAL--LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLES 60

Query: 61  DLLHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAE 120
           +       + S  +  ++   D     K   E   +R           Q +  R+++ A 
Sbjct: 61  EFESGSDSESSSSITLNLDHIDALSSNKTPDELFSSR----------LQRDSRRVKSIAT 120

Query: 121 AATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLT 180
            A Q+        P          ++SG   GS EYF +L VGTPA+   ++ DTGSD+ 
Sbjct: 121 LAAQIPGRNVTHAPRPGG--FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIV 180

Query: 181 WLKCR-YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELF 240
           WL+C   RRC+       + +                +S T+ T+ CSS  C        
Sbjct: 181 WLQCAPCRRCYSQSDPIFDPR----------------KSKTYATIPCSSPHCRR-----L 240

Query: 241 AVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNV 300
             A C+T    C+Y  SY  G+   G F+ ETLT      +  +++   +GC    +G +
Sbjct: 241 DSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTF-----RRNRVKGVALGCGHDNEG-L 300

Query: 301 FDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSAST 360
           F GA G++GLG    S   +     N   FSYCLVD     +  S  V G    S  A  
Sbjct: 301 FVGAAGLLGLGKGKLSFPGQTGHRFN-QKFSYCLVDRSASSKP-SSVVFGNAAVSRIA-- 360

Query: 361 SSAKPPAKMSYTKLYVGDPYSSFYGVDLIGISADG-QMLNIPPRVW--DSYKGCGTIIDS 420
                     +T L       +FY V L+GIS  G ++  +   ++  D     G IIDS
Sbjct: 361 ---------RFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDS 420

Query: 421 GTSLTVLATPAFDVVMEVLTSRLKQFQQI-EIEPFNFCFNNSQYTHDMAPKLRFHFGDGT 480
           GTS+T L  PA+  + +      K  ++  +   F+ CF+ S       P +  HF    
Sbjct: 421 GTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGAD 480

Query: 481 VFEPPTKSYIVSV---GEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAAS 530
           V  P T +Y++ V   G+F  C          L+IIGNI QQ     +D    RVGFA  
Sbjct: 481 VSLPAT-NYLIPVDTNGKF--CFAFAG-TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPG 484

BLAST of IVF0000691 vs. ExPASy TrEMBL
Match: A0A1S3C2F3 (aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.7e-307
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60
           MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR
Sbjct: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60

Query: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120
           HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV
Sbjct: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120

Query: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180
           EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR
Sbjct: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180

Query: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240
           YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD
Sbjct: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240

Query: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300
           TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG
Sbjct: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300

Query: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360
           VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP
Sbjct: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360

Query: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420
           AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT
Sbjct: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420

Query: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480
           PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI
Sbjct: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480

Query: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 531
           VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI
Sbjct: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530

BLAST of IVF0000691 vs. ExPASy TrEMBL
Match: A0A0A0KG92 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.9e-273
Identity = 473/537 (88.08%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFC---FFFLLLFFLSFSSSFLFALGDEANNYNN----NDDEDEQQTI 60
           MLGYRKPMSPISNFC   FFFLL FFLSFSSSFLFALGDE NN+NN    NDDEDEQ+ I
Sbjct: 1   MLGYRKPMSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEII 60

Query: 61  RFDLLHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAE 120
           +FDLLHRHHPQV+EK++GDMKIQD+ ERMKDIHEHD NRHRSISKSMNQKQ+EDARLRAE
Sbjct: 61  KFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAE 120

Query: 121 AEAATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSD 180
           AEAAT+ EVAKSAILPPATSTPIGM+MISGADFGSSEYFV+LKVGTPAQTFMLIADTGSD
Sbjct: 121 AEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSD 180

Query: 181 LTWLKCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAEL 240
           LTW+KCRYRRCFGNCS NVNHKSKNEKKQRFRHA LAN SS+FKTVSCSSTMCTN+LA+L
Sbjct: 181 LTWMKCRYRRCFGNCSSNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLADL 240

Query: 241 FAVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGN 300
           FAV EC  PTSPCVYDYSY GGASAKGIFAWETLTVGLTNGKEKQL NSIIGCTE VQG+
Sbjct: 241 FAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQGS 300

Query: 301 VFDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSAS 360
           VF GADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRA+SYFVLG+PTPSTSAS
Sbjct: 301 VFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPSTSAS 360

Query: 361 TSSAKPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGT 420
           TSSAK PAKM+YTKLYVGDPYSSFYGVDLIGISA+G MLNIP RVWD   G GTIIDSGT
Sbjct: 361 TSSAKLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIIDSGT 420

Query: 421 SLTVLATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFE 480
           SLT+LA PAFD+VME LT RLK+FQQ+EIEPF+FCFNNSQYTH+MAPKLRFHFGDGTVFE
Sbjct: 421 SLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFGDGTVFE 480

Query: 481 PPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 531
           PPTKSYIVSVG+FISCIG VSMPFP+ NIIGNILQQNHLWQFDFQKRRVGFA SECI
Sbjct: 481 PPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSECI 537

BLAST of IVF0000691 vs. ExPASy TrEMBL
Match: A0A5D3B701 (Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00400 PE=3 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.0e-264
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 73  MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 132
           MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT
Sbjct: 1   MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 60

Query: 133 STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV 192
           STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV
Sbjct: 61  STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV 120

Query: 193 NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY 252
           NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY
Sbjct: 121 NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY 180

Query: 253 AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT 312
           AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT
Sbjct: 181 AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT 240

Query: 313 YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD 372
           YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD
Sbjct: 241 YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD 300

Query: 373 PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS 432
           PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS
Sbjct: 301 PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS 360

Query: 433 RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI 492
           RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI
Sbjct: 361 RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI 420

Query: 493 VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 531
           VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI
Sbjct: 421 VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 458

BLAST of IVF0000691 vs. ExPASy TrEMBL
Match: A0A6J1FXD5 (aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 3.1e-157
Identity = 282/530 (53.21%), Postives = 356/530 (67.17%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60
           MLGY  PMSPIS    FF   FFLS   +F      + +         E   ++ D++HR
Sbjct: 1   MLGYTNPMSPISPLLIFFFFNFFLSVHVAF------DGDEQQQQQKPSEMPMVKLDVMHR 60

Query: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120
           HHP V EKL G+ +     +R +DIHEHD NR RSIS SM   + +              
Sbjct: 61  HHPHVQEKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ------------ 120

Query: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180
                  LP  +S PI +K+ SG DFG++EYFVQ +VGTP Q F+LI DTGSDLTWLKCR
Sbjct: 121 -------LPMPSSAPIQLKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCR 180

Query: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240
           YRRC GNC+ + +HKS+ E K +F H  LAN SS+FK ++C S  C  +L  LFA+ +C 
Sbjct: 181 YRRCLGNCTAHAHHKSRVEHKVKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQ 240

Query: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300
            P++PCVYDYSY GG +A G+FA ET+TVGLTNGKEKQL +++IGCTE+       G DG
Sbjct: 241 VPSNPCVYDYSYIGGGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDG 300

Query: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360
           ++GLGT ++S  ++AA + NGGGFSYCL+DHL+   A SYF+LG P     A   S  P 
Sbjct: 301 ILGLGTGAHSFAHRAALDKNGGGFSYCLIDHLSHHSATSYFILGYP----PAEPLSVAPV 360

Query: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420
             M++  L++G P++S+YGV LIGIS DG  LNIPPRVWD  KG GTI+DSGTSL++L  
Sbjct: 361 GNMTFINLHLGGPFNSYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTA 420

Query: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480
           PAFDV ME +  +LK+FQQI  +PF +CFN + Y+H+MAPKLRFHF  G VFEPP KSYI
Sbjct: 421 PAFDVFMEAMVQKLKKFQQILADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYI 480

Query: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 531
           V V + I C+G  S+PFP  NIIGNILQQN LWQFDF  ++VGFA S+CI
Sbjct: 481 VKVDD-ILCLGFTSIPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQCI 500

BLAST of IVF0000691 vs. ExPASy TrEMBL
Match: A0A6J1FVB3 (aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 9.5e-154
Identity = 277/523 (52.96%), Postives = 351/523 (67.11%), Query Frame = 0

Query: 8   MSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHRHHPQVSE 67
           MSPIS    FF   FFLS   +F      + +         E   ++ D++HRHHP V E
Sbjct: 1   MSPISPLLIFFFFNFFLSVHVAF------DGDEQQQQQKPSEMPMVKLDVMHRHHPHVQE 60

Query: 68  KLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAI 127
           KL G+ +     +R +DIHEHD NR RSIS SM   + +                     
Sbjct: 61  KLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ------------------- 120

Query: 128 LPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGN 187
           LP  +S PI +K+ SG DFG++EYFVQ +VGTP Q F+LI DTGSDLTWLKCRYRRC GN
Sbjct: 121 LPMPSSAPIQLKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGN 180

Query: 188 CSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCV 247
           C+ + +HKS+ E K +F H  LAN SS+FK ++C S  C  +L  LFA+ +C  P++PCV
Sbjct: 181 CTAHAHHKSRVEHKVKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQVPSNPCV 240

Query: 248 YDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTS 307
           YDYSY GG +A G+FA ET+TVGLTNGKEKQL +++IGCTE+       G DG++GLGT 
Sbjct: 241 YDYSYIGGGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTG 300

Query: 308 SYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTK 367
           ++S  ++AA + NGGGFSYCL+DHL+   A SYF+LG P     A   S  P   M++  
Sbjct: 301 AHSFAHRAALDKNGGGFSYCLIDHLSHHSATSYFILGYP----PAEPLSVAPVGNMTFIN 360

Query: 368 LYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVM 427
           L++G P++S+YGV LIGIS DG  LNIPPRVWD  KG GTI+DSGTSL++L  PAFDV M
Sbjct: 361 LHLGGPFNSYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFM 420

Query: 428 EVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFI 487
           E +  +LK+FQQI  +PF +CFN + Y+H+MAPKLRFHF  G VFEPP KSYIV V + I
Sbjct: 421 EAMVQKLKKFQQILADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIVKVDD-I 480

Query: 488 SCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 531
            C+G  S+PFP  NIIGNILQQN LWQFDF  ++VGFA S+CI
Sbjct: 481 LCLGFTSIPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQCI 493

BLAST of IVF0000691 vs. NCBI nr
Match: XP_008456273.1 (PREDICTED: aspartic proteinase CDR1 [Cucumis melo])

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 530/530 (100.00%), Postives = 530/530 (100.00%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60
           MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR
Sbjct: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60

Query: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120
           HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV
Sbjct: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120

Query: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180
           EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR
Sbjct: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180

Query: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240
           YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD
Sbjct: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240

Query: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300
           TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG
Sbjct: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300

Query: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360
           VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP
Sbjct: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360

Query: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420
           AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT
Sbjct: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420

Query: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480
           PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI
Sbjct: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480

Query: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530
           VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI
Sbjct: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530

BLAST of IVF0000691 vs. NCBI nr
Match: XP_004140022.2 (aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical protein Csa_021058 [Cucumis sativus])

HSP 1 Score: 944 bits (2440), Expect = 0.0
Identity = 473/537 (88.08%), Postives = 501/537 (93.30%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFF---LLLFFLSFSSSFLFALGDEANNYNNN----DDEDEQQTI 60
           MLGYRKPMSPISNFCFFF   LL FFLSFSSSFLFALGDE NN+NNN    DDEDEQ+ I
Sbjct: 1   MLGYRKPMSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEII 60

Query: 61  RFDLLHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAE 120
           +FDLLHRHHPQV+EK++GDMKIQD+ ERMKDIHEHD NRHRSISKSMNQKQ+EDARLRAE
Sbjct: 61  KFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAE 120

Query: 121 AEAATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSD 180
           AEAAT+ EVAKSAILPPATSTPIGM+MISGADFGSSEYFV+LKVGTPAQTFMLIADTGSD
Sbjct: 121 AEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSD 180

Query: 181 LTWLKCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAEL 240
           LTW+KCRYRRCFGNCS NVNHKSKNEKKQRFRHA LAN SS+FKTVSCSSTMCTN+LA+L
Sbjct: 181 LTWMKCRYRRCFGNCSSNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLADL 240

Query: 241 FAVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGN 300
           FAV EC  PTSPCVYDYSY GGASAKGIFAWETLTVGLTNGKEKQL NSIIGCTE VQG+
Sbjct: 241 FAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQGS 300

Query: 301 VFDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSAS 360
           VF GADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRA+SYFVLG+PTPSTSAS
Sbjct: 301 VFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPSTSAS 360

Query: 361 TSSAKPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGT 420
           TSSAK PAKM+YTKLYVGDPYSSFYGVDLIGISA+G MLNIP RVWD   G GTIIDSGT
Sbjct: 361 TSSAKLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIIDSGT 420

Query: 421 SLTVLATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFE 480
           SLT+LA PAFD+VME LT RLK+FQQ+EIEPF+FCFNNSQYTH+MAPKLRFHFGDGTVFE
Sbjct: 421 SLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFGDGTVFE 480

Query: 481 PPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530
           PPTKSYIVSVG+FISCIG VSMPFP+ NIIGNILQQNHLWQFDFQKRRVGFA SECI
Sbjct: 481 PPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSECI 537

BLAST of IVF0000691 vs. NCBI nr
Match: KAA0033565.1 (aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic proteinase CDR1 [Cucumis melo var. makuwa])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0

Query: 73  MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 132
           MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT
Sbjct: 1   MKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPAT 60

Query: 133 STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV 192
           STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV
Sbjct: 61  STPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNV 120

Query: 193 NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY 252
           NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY
Sbjct: 121 NHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSY 180

Query: 253 AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT 312
           AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT
Sbjct: 181 AGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLT 240

Query: 313 YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD 372
           YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD
Sbjct: 241 YKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGD 300

Query: 373 PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS 432
           PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS
Sbjct: 301 PYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTS 360

Query: 433 RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI 492
           RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI
Sbjct: 361 RLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGI 420

Query: 493 VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530
           VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI
Sbjct: 421 VSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 458

BLAST of IVF0000691 vs. NCBI nr
Match: XP_038901983.1 (aspartic proteinase NANA, chloroplast [Benincasa hispida])

HSP 1 Score: 749 bits (1933), Expect = 8.55e-268
Identity = 371/532 (69.74%), Postives = 440/532 (82.71%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60
           MLGYRKPMSPIS+FC     LFFL F  S   A GD +++         Q+ ++ DLLHR
Sbjct: 1   MLGYRKPMSPISHFC-----LFFLFFFLSVPIAFGDGSHD---------QENVKLDLLHR 60

Query: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120
           HHPQVSEKL+GD+K++++++R+KDI EHD+ R+++IS S+N+ ++ D +LR EA    + 
Sbjct: 61  HHPQVSEKLHGDIKLENMNDRIKDILEHDQKRYQTISSSLNRNEL-DEQLRKEAAELAE- 120

Query: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180
              K   LPP +STPIG+KMISG+D+GSSEYFVQLKVGTP QTFMLIADTGSDLTW+KCR
Sbjct: 121 ---KDLKLPPISSTPIGLKMISGSDYGSSEYFVQLKVGTPPQTFMLIADTGSDLTWMKCR 180

Query: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240
           YRRC GNCS N NHK++NE+K RFR+A LAN SS+FKT+ CSS MCTN+LA+LF++ EC 
Sbjct: 181 YRRCIGNCSSNPNHKTRNERKVRFRNAFLANYSSSFKTIDCSSKMCTNDLADLFSIGECQ 240

Query: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300
           TPTSPC+YDYSY+GGASAKG+FA ETLTVGLTNGKEKQL NSIIGCTE VQG +F GADG
Sbjct: 241 TPTSPCLYDYSYSGGASAKGLFAIETLTVGLTNGKEKQLHNSIIGCTESVQGRIFGGADG 300

Query: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPST--SASTSSAK 360
           V+GLGTSSYS TYKAAENANGGGF+YCLVDHL+D+ A SYF+LG P  ST  +A+ SS  
Sbjct: 301 VIGLGTSSYSFTYKAAENANGGGFAYCLVDHLSDRTATSYFILGNPISSTDSAAAASSVA 360

Query: 361 PPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVL 420
           P   MS+TKL++GDPYSSFYGVDL+GISADG MLNIPPRVWD   G GTI+DSGTSLT+L
Sbjct: 361 PTGNMSFTKLFLGDPYSSFYGVDLVGISADGVMLNIPPRVWDINSGGGTIVDSGTSLTML 420

Query: 421 ATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKS 480
           A PAFD+VME L  +LK F+ IEIEPF+FCFNNS+YTH+MAPKLRFHFGDGTVF+PP KS
Sbjct: 421 AAPAFDMVMEALVPKLKHFENIEIEPFDFCFNNSRYTHEMAPKLRFHFGDGTVFQPPPKS 480

Query: 481 YIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530
           YIVSVGE+ISCIG VSMPFP+ NIIGNILQQNHLW+FDF    VGFA SEC+
Sbjct: 481 YIVSVGEYISCIGFVSMPFPATNIIGNILQQNHLWKFDFHAGTVGFAPSECV 513

BLAST of IVF0000691 vs. NCBI nr
Match: XP_022943788.1 (aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 560 bits (1443), Expect = 1.44e-193
Identity = 284/530 (53.58%), Postives = 357/530 (67.36%), Query Frame = 0

Query: 1   MLGYRKPMSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDLLHR 60
           MLGY  PMSPIS    FF   FFLS   +F    GDE           E   ++ D++HR
Sbjct: 1   MLGYTNPMSPISPLLIFFFFNFFLSVHVAFD---GDEQQQQQK---PSEMPMVKLDVMHR 60

Query: 61  HHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQV 120
           HHP V EKL G+ +     +R +DIHEHD NR RSIS SM   + +              
Sbjct: 61  HHPHVQEKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQ------------ 120

Query: 121 EVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCR 180
                  LP  +S PI +K+ SG DFG++EYFVQ +VGTP Q F+LI DTGSDLTWLKCR
Sbjct: 121 -------LPMPSSAPIQLKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCR 180

Query: 181 YRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECD 240
           YRRC GNC+ + +HKS+ E K +F H  LAN SS+FK ++C S  C  +L  LFA+ +C 
Sbjct: 181 YRRCLGNCTAHAHHKSRVEHKVKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQ 240

Query: 241 TPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADG 300
            P++PCVYDYSY GG +A G+FA ET+TVGLTNGKEKQL +++IGCTE+       G DG
Sbjct: 241 VPSNPCVYDYSYIGGGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDG 300

Query: 301 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 360
           ++GLGT ++S  ++AA + NGGGFSYCL+DHL+   A SYF+LG P     A   S  P 
Sbjct: 301 ILGLGTGAHSFAHRAALDKNGGGFSYCLIDHLSHHSATSYFILGYP----PAEPLSVAPV 360

Query: 361 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 420
             M++  L++G P++S+YGV LIGIS DG  LNIPPRVWD  KG GTI+DSGTSL++L  
Sbjct: 361 GNMTFINLHLGGPFNSYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTA 420

Query: 421 PAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYI 480
           PAFDV ME +  +LK+FQQI  +PF +CFN + Y+H+MAPKLRFHF  G VFEPP KSYI
Sbjct: 421 PAFDVFMEAMVQKLKKFQQILADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYI 480

Query: 481 VSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASECI 530
           V V + + C+G  S+PFP  NIIGNILQQN LWQFDF  ++VGFA S+CI
Sbjct: 481 VKVDDIL-CLGFTSIPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQCI 500

BLAST of IVF0000691 vs. TAIR 10
Match: AT3G12700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 304.3 bits (778), Expect = 1.9e-82
Identity = 171/455 (37.58%), Postives = 258/455 (56.70%), Query Frame = 0

Query: 80  ERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATSTPIGMK 139
           + MKD     +  HR         +IED             +  + +++    ++ +G+K
Sbjct: 42  DSMKDTSVRLKLAHRDTLLPKPLSRIED---------VIGADQKRHSLISRKRNSTVGVK 101

Query: 140 MI--SGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVNHKSK 199
           M   SG D+G+++YF +++VGTPA+ F ++ DTGS+LTW+ CRYR       G  N    
Sbjct: 102 MDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRYR-----ARGKDN---- 161

Query: 200 NEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYAGGAS 259
                  R    A++S +FKTV C +  C  +L  LF++  C TP++PC YDY YA G++
Sbjct: 162 -------RRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSA 221

Query: 260 AKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAE 319
           A+G+FA ET+TVGLTNG+  +L   +IGC+    G  F GADGV+GL  S +S T   A 
Sbjct: 222 AQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQGADGVLGLAFSDFSFT-STAT 281

Query: 320 NANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDPYSSF 379
           +  G  FSYCLVDHL+++   +Y + G    S+ ++ ++ +    +  T++        F
Sbjct: 282 SLYGAKFSYCLVDHLSNKNVSNYLIFG----SSRSTKTAFRRTTPLDLTRI------PPF 341

Query: 380 YGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRLKQF 439
           Y +++IGIS    ML+IP +VWD+  G GTI+DSGTSLT+LA  A+  V+  L   L + 
Sbjct: 342 YAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVEL 401

Query: 440 QQIEIE--PFNFCFN-NSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVS 499
           ++++ E  P  +CF+  S +     P+L FH   G  FEP  KSY+V     + C+G VS
Sbjct: 402 KRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVS 460

Query: 500 MPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
              P+ N+IGNI+QQN+LW+FD     + FA S C
Sbjct: 462 AGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of IVF0000691 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 212.6 bits (540), Expect = 7.6e-55
Identity = 139/403 (34.49%), Postives = 202/403 (50.12%), Query Frame = 0

Query: 140 MISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVNHKSKNE 199
           ++SGA  GS +YFV L++G P Q+ +LIADTGSDL W+KC   R   NCS    H S   
Sbjct: 73  VVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACR---NCS----HHSP-- 132

Query: 200 KKQRFRHALLANQSSTFKTVSCSSTMC-TNNLAELFAVAECDTPTSPCVYDYSYAGGASA 259
                        SSTF    C   +C      +   +       S C Y+Y YA G+  
Sbjct: 133 -----ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLT 192

Query: 260 KGIFAWETLTVGLTNGKEKQLRNSIIGC-----TEIVQGNVFDGADGVMGLGTSSYSLTY 319
            G+FA ET ++  ++GKE +L++   GC      + V G  F+GA+GVMGLG    S   
Sbjct: 193 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFAS 252

Query: 320 KAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDP 379
           +      G  FSYCL+D+       SY ++G          +     +K+ +T L     
Sbjct: 253 QLGRRF-GNKFSYCLMDYTLSPPPTSYLIIG----------NGGDGISKLFFTPLLTNPL 312

Query: 380 YSSFYGVDLIGISADGQMLNIPPRVW---DSYKGCGTIIDSGTSLTVLATPAFDVVMEVL 439
             +FY V L  +  +G  L I P +W   DS  G GT++DSGT+L  LA PA+  V+  +
Sbjct: 313 SPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNG-GTVVDSGTTLAFLAEPAYRSVIAAV 372

Query: 440 TSRLKQFQQIEIEP-FNFCFNNSQYT--HDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFI 499
             R+K      + P F+ C N S  T    + P+L+F F  G VF PP ++Y +   E I
Sbjct: 373 RRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQI 432

Query: 500 SCIGIVSM-PFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
            C+ I S+ P    ++IGN++QQ  L++FD  + R+GF+   C
Sbjct: 433 QCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGC 449

BLAST of IVF0000691 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 179.1 bits (453), Expect = 9.3e-45
Identity = 140/495 (28.28%), Postives = 230/495 (46.46%), Query Frame = 0

Query: 49  EQQTIRFDLLHRHHPQVSEKLNG---DMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQI 108
           E +T++F L  R      +       +++I+DL  R++ +       H+ + +  NQ  +
Sbjct: 76  ENKTVKFHLKRRETTTTEKATTNSVLELQIRDL-TRIQTL-------HKRVLEKNNQNTV 135

Query: 109 EDARLRAEAEAATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFM 168
              + + + E  T   VA S       +  +   + SG   GS EYF+ + VG+P + F 
Sbjct: 136 SQKQKKNDKEVVTTTPVASSV---EEQAGQLVATLESGMTLGSGEYFMDVLVGSPPKHFS 195

Query: 169 LIADTGSDLTWLKC-RYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSST 228
           LI DTGSDL W++C     CF       + K+                S+++K ++C+  
Sbjct: 196 LILDTGSDLNWIQCLPCYDCFQQNGAFYDPKA----------------SASYKNITCNDQ 255

Query: 229 MCTNNLAELFAVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGL-TNGKEKQL---R 288
            C N ++       C +    C Y Y Y   ++  G FA ET TV L TNG   +L    
Sbjct: 256 RC-NLVSSPDPPMPCKSDNQSCPYYYWYGDSSNTTGDFAVETFTVNLTTNGGSSELYNVE 315

Query: 289 NSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSY 348
           N + GC    +G +F GA G++GLG    S +    ++  G  FSYCLVD  +D    S 
Sbjct: 316 NMMFGCGHWNRG-LFHGAAGLLGLGRGPLSFS-SQLQSLYGHSFSYCLVDRNSDTNVSSK 375

Query: 349 FVLGVPTPSTSASTSSAKPPAKMSYTKLYVG--DPYSSFYGVDLIGISADGQMLNIPPRV 408
            + G      S           +++T    G  +   +FY V +  I   G++LNIP   
Sbjct: 376 LIFGEDKDLLS--------HPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEET 435

Query: 409 WD--SYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRLKQFQQI--EIEPFNFCFNNSQY 468
           W+  S    GTIIDSGT+L+  A PA++ +   +  + K    +  +    + CFN S  
Sbjct: 436 WNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFPILDPCFNVSGI 495

Query: 469 THDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQ 528
            +   P+L   F DG V+  PT++  + + E + C+ ++  P  + +IIGN  QQN    
Sbjct: 496 HNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPKSAFSIIGNYQQQNFHIL 532

Query: 529 FDFQKRRVGFAASEC 530
           +D ++ R+G+A ++C
Sbjct: 556 YDTKRSRLGYAPTKC 532

BLAST of IVF0000691 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.5e-42
Identity = 135/471 (28.66%), Postives = 218/471 (46.28%), Query Frame = 0

Query: 72  DMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPA 131
           D++IQDL  R+K +H       +  ++ + +K   D  L    E +    +A        
Sbjct: 97  DLQIQDL-TRIKTLHARFNKSKKQKNEKVRKKITSDISLVGAPEVSPGKLIA-------- 156

Query: 132 TSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKC-RYRRCFGNCSG 191
                   + SG   GS EYF+ + VGTP + F LI DTGSDL WL+C     CF     
Sbjct: 157 -------TLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDCFHQNGM 216

Query: 192 NVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDY 251
             + K+                S++FK ++C+   C + ++      +C++    C Y Y
Sbjct: 217 FYDPKT----------------SASFKNITCNDPRC-SLISSPDPPVQCESDNQSCPYFY 276

Query: 252 SYAGGASAKGIFAWETLTVGLT----NGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGT 311
            Y   ++  G FA ET TV LT       E ++ N + GC    +G +F GA G++GLG 
Sbjct: 277 WYGDRSNTTGDFAVETFTVNLTTTEGGSSEYKVGNMMFGCGHWNRG-LFSGASGLLGLGR 336

Query: 312 SSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYT 371
              S +    ++  G  FSYCLVD  ++    S  + G                  +++T
Sbjct: 337 GPLSFS-SQLQSLYGHSFSYCLVDRNSNTNVSSKLIFG--------EDKDLLNHTNLNFT 396

Query: 372 KLYVGDPYS--SFYGVDLIGISADGQMLNIPPRVWD--SYKGCGTIIDSGTSLTVLATPA 431
               G   S  +FY + +  I   G+ L+IP   W+  S    GTIIDSGT+L+  A PA
Sbjct: 397 SFVNGKENSVETFYYIQIKSILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPA 456

Query: 432 FDVVMEVLTSRLKQFQQI--EIEPFNFCFNNS--QYTHDMAPKLRFHFGDGTVFEPPTKS 491
           ++++      ++K+   I  +    + CFN S  +  +   P+L   F DGTV+  P ++
Sbjct: 457 YEIIKNKFAEKMKENYPIFRDFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAEN 516

Query: 492 YIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 530
             + + E + C+ I+  P  + +IIGN  QQN    +D ++ R+GF  ++C
Sbjct: 517 SFIWLSEDLVCLAILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of IVF0000691 vs. TAIR 10
Match: AT3G59080.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.6e-39
Identity = 134/494 (27.13%), Postives = 214/494 (43.32%), Query Frame = 0

Query: 49  EQQTIRFDLLHRHHPQVSEKLNG---DMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQI 108
           E +T++F L  R      +       +++I+DL  R++ +       H+ + +  NQ  +
Sbjct: 76  ENKTVKFHLKRRETTTTEKATTNSVLELQIRDL-TRIQTL-------HKRVLEKNNQNTV 135

Query: 109 EDARLRAEAEAATQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFM 168
              + + + E  T   VA S       +  +   + SG   GS EYF+ + VG+P + F 
Sbjct: 136 SQKQKKNDKEVVTTTPVASSV---EEQAGQLVATLESGMTLGSGEYFMDVLVGSPPKHFS 195

Query: 169 LIADTGSDLTWLKCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTM 228
           LI DTGSDL W++C    C+     N N                                
Sbjct: 196 LILDTGSDLNWIQC--LPCYDCFQQNDNQS------------------------------ 255

Query: 229 CTNNLAELFAVAECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGL-TNGKEKQL---RN 288
                               C Y Y Y   ++  G FA ET TV L TNG   +L    N
Sbjct: 256 --------------------CPYYYWYGDSSNTTGDFAVETFTVNLTTNGGSSELYNVEN 315

Query: 289 SIIGCTEIVQGNVFDGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYF 348
            + GC    +G +F GA G++GLG    S +    ++  G  FSYCLVD  +D    S  
Sbjct: 316 MMFGCGHWNRG-LFHGAAGLLGLGRGPLSFS-SQLQSLYGHSFSYCLVDRNSDTNVSSKL 375

Query: 349 VLGVPTPSTSASTSSAKPPAKMSYTKLYVG--DPYSSFYGVDLIGISADGQMLNIPPRVW 408
           + G      S           +++T    G  +   +FY V +  I   G++LNIP   W
Sbjct: 376 IFGEDKDLLS--------HPNLNFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETW 435

Query: 409 D--SYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSRLKQFQQI--EIEPFNFCFNNSQYT 468
           +  S    GTIIDSGT+L+  A PA++ +   +  + K    +  +    + CFN S   
Sbjct: 436 NISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFPILDPCFNVSGIH 495

Query: 469 HDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQF 528
           +   P+L   F DG V+  PT++  + + E + C+ ++  P  + +IIGN  QQN    +
Sbjct: 496 NVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPKSAFSIIGNYQQQNFHILY 496

Query: 529 DFQKRRVGFAASEC 530
           D ++ R+G+A ++C
Sbjct: 556 DTKRSRLGYAPTKC 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTW42.7e-8137.58Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Q9LS402.9e-3529.29Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
O044961.2e-3128.82Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1[more]
Q6XBF81.2e-3129.87Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q9LNJ32.8e-3028.04Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A1S3C2F33.7e-307100.00aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1[more]
A0A0A0KG925.9e-27388.08Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G13439... [more]
A0A5D3B7011.0e-264100.00Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1FXD53.1e-15753.21aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1FVB39.5e-15452.96aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
XP_008456273.10.0100.00PREDICTED: aspartic proteinase CDR1 [Cucumis melo][more]
XP_004140022.20.088.08aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical... [more]
KAA0033565.10.0100.00aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic protein... [more]
XP_038901983.18.55e-26869.74aspartic proteinase NANA, chloroplast [Benincasa hispida][more]
XP_022943788.11.44e-19353.58aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G12700.11.9e-8237.58Eukaryotic aspartyl protease family protein [more]
AT3G25700.17.6e-5534.49Eukaryotic aspartyl protease family protein [more]
AT3G59080.19.3e-4528.28Eukaryotic aspartyl protease family protein [more]
AT2G42980.11.5e-4228.66Eukaryotic aspartyl protease family protein [more]
AT3G59080.22.6e-3927.13Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 157..177
score: 47.32
coord: 501..516
score: 23.01
coord: 407..418
score: 46.5
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 151..344
e-value: 4.9E-43
score: 147.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 356..530
e-value: 1.1E-38
score: 134.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 134..344
e-value: 5.6E-38
score: 132.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 143..529
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 378..525
e-value: 8.7E-29
score: 100.4
NoneNo IPR availablePANTHERPTHR47967:SF69ASPARTIC PROTEINASE NANA, CHLOROPLASTcoord: 18..529
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 18..529
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 166..177
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 151..525
score: 35.87019
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 150..529
e-value: 4.74192E-73
score: 231.383

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000691.1IVF0000691.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity