IVF0000442 (gene) Melon (IVF77) v1

Overview
NameIVF0000442
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPectinesterase
Locationchr02: 2663735 .. 2668831 (+)
RNA-Seq ExpressionIVF0000442
SyntenyIVF0000442
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTACTACAAAGTCCTCTCCCTTCTCTTATTTTATCCCGAGTCCAAAAAACCGAAACACGAGGAGATTTTCGGGAGAGAATGTTTCGGCCGACCGTTGCTTCATCTTTTTCGGCGACGGTGGCGTTGGCATTGGCGATGATGTTCCAACTCATAATCCTAATAACTATTCCGTCCACCGTCCTCGTCGAAGCTCGCACGAAAACAATTCCGGCAGACTCGTCGAAGCTGGACGAATGGATCGCACACAACATGAGAGAATACAACGATCGTAAGATGAACGAGACAGGAATCAAGGCGCTGGACCGGCGGCTGGCTGAGGCGGAGGATTGCGTTCGATTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCTCGACCATCACTGAAGCGATCGAAAGCATTCCGTCAGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGAGGTGGAGTTTATAGAGAGAAAATTACGATCGATGCTTCCAAGCCGTTTGTGACTTTGTATGGTCAGAAAGGAAAACGGCCAGTGATAACATTCGACGGTACGGCGTCGGAGTTTGGTACCGTTAAGAGTGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTACGTTTGTGGTGAGTTTTTCTTCTTCTTCTTCTTTTTAATTTTGTGGGGTACGTTTTGTAAATAGTAATAATTATGGGTCTATAAGTTTTTGGTAACGTAGGATGTGCATGAGTTTTTCATTTTTTCTTTTTTAAATTTAAATACTCATGCAATTCACTCGTCATCAAAATTATTCCATACTCATGCTATTCCTCATATGTACAAAACATGATTTTTCACGTTGAAATCTTGACCTTTTTCTTGCTCGATTGGCTATATATCTATATGAAAAGAAATTTATTTAATAAGATCGCTACCCCTATTAATGGTTGAGTGTAGGTAGAAAATGTAAGTGAGAATTGTTTTTCTCAAATTTAGTAAAATATCATAATTTATCTTTCATACATTACGATGGACTATTATATGCATTTATCATGATACTACAGACATATGTGGTGACCTTTATTATGATTGTTTATAGATAAACAATGATATTTTACTATGATTATAAATATTTTCAGTAGTTGTATAATTTAAAATAATGTTTGCGTATAATAATTACTACGGTATGTAAATTCATGTGTTGGTATAAGAACTATACATAGTGTTTGAGATGGACTATGTTTACTTAATTGAATTGTGAGTGAGAATAAGTTAGGTTTGATTTGATAACTATTTTGGTTTTAGATTTTTGTTTTTGAAATTTGAGGTAGTTTTCTTTCCATTTTTCAACCATATAGTTTTTATAATTTTTTAAGAAACTATTGACTTCTAAGTCAAATTCCAGAAGCTAAAACAAGTTTTTGAAAATTATTTTTTTAGTTTTCAAACTTTAGTCAGAAAGAAGATGCAAAAGCATAAAAATCCTTAAAAAATAGTGTTTTAAGTTTAAGTTTGGTTTTAGACGTTTTGAAAAAAGTTTAGAATTTAGTACCTGTATTTTTAACAAATAAAACTTAGCCCTTATAGCTAGTTTGATATATAGAACCTCGTAAAGTCATCTTGATGGTGTGATAAAATCTTCATAGAAATTAGTTATGGAGTTCTATAAAATTTAGTCGTGGTTTGATGATATTCTCATAAATAGTTCACATAGGTTAGAGAAGAGGCTATTTGTGATTTATTTTTATTTTTATAAATACTAAATTAATTCTTAACTTCTTTTTTTCAAATATAGGACCAAATTTATAATTTGACGTTTTCAGTAGAGATAAAGGGATTCAAACTACATACCTTTTTAATCGCTAATTATAAACATTTGATACGTACATGTTTTAGAGCTATGTTTACATTATCTTTAATTATCATCTATTTTGTAGTCCGGTAGAAAGGAATTAGGTTAATAATGTGGTTAGGGTTGGTAGCAATCCTAACATGTTTATTTGTTTCACAGGTATATATACTACAGGGTGATACCTTTACTAGCTAGGTGATCAGGTTTGAAAAAATAGAAAGAATAGAATTAAAGTTGGCTACTTTCCATTTTAAAGAATGTGCAATTTGGTGTGAACGACTTTAATTTTTTAGTATTTATCAAAGTTCATTTCTTAGAATTGGAAAAGTGAATGTGTATAGAAAAGGTAATGGAAAGAAGGATTTATTGACAAATCCCACCCAAGATTATTATAAAAACGTGAGAGAGAGGCAGTTGCATTTCAGAATTAATTAACTGTAAGAAAAGTAATTCACGTTTTTTATAAGCGTCCGGTTAAGATGCTACCTCTTTTGAATTAGTTCACCATTTTAGGGTACATTTAATCCTATTTTCTTGGACACTGATACATATATTAGATAAACACTAGTTTCATCTTAAAAGTGATCAACTAACAATAGAAGAAATAACCCGTGTATTTATTATAAAGATTATTACGAGATCTCTTCGATCTTCAACAATTATAACAACAAGGTATGAGAGGAGTATATGAATGAACCAATATCACATTTGAAGTAAAGAAATTATGAAGATATGAAATACCACCTAAAAGACACCCACATGAAGATTAACTTTTCAATCTAGAGGCCAAATTATCATAAGAATTCCAAACTTAACCTTGTTTAGCTTGAAGCAACCCAATGTTTCAAGAGAAGTAAGAATTACATAGTCAGGTCGCAAAGTAAATACGAAGGTAATTAGCTACTTAATCCTGGCATGAGGCATTGTAGTTTTTGAAAAAATTAAAAGTGAGAATTTGTAAGAAACCATATATATAACATTATATTAGTAAAAAGTGAATTTATATAGAAGAAATGAAGTTTGGTTGTAAATTTGTTGTGCAGAATTCAGCTCCAATGCCAGAACTAGGAGGAACGGGAGGGCAAGCAGTGGCGATGAGGATATCTGGGGACAAGGCAGCATTCCATGAGTGTCATTTCATAGGCTTTCAAGACACTTTATGTGATGACAGAGGCAGACATTTTTTCAGGGATTGTTACATCCAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCTCTCTACTTGGTATGTTATACATCCGCTTTTTATACTTTGAAATAAATAAAAAAAACGGTAAGGTATTCGTAAACTTACATGACAGGATACTTGGTGATCAACTAATAGAAATTTGGATGAGGAATTAAGTCAACTTTAGGAGAAGAAAACGTCCCAATTGGTAACACTCTTGGGTTCCCGATAGTTTAAGCCCACTTGATAATCATTTACCTTATTTTATTTTAGTTTTTCTTTTGAAAATTAAGTCCACAAATCACTCCTTTCATTTCTAAACTTGTTGATCTATTATCCACTTTTTTCGTCAATTTTTATAGAAAACGAAACGATTACCCAAATGGATCGAGACATAAGTGACAGTTTATTTGTATTTTTGATGTATGAGAAAGAAGTGCCTCAAAATTTATATCTAGAAGTTAAAAATGAGTGTTTTGACCCAAAAGGTTGAGAGTAGAAGTCGAGAGAAGTGGTCCTTGTTTGTACTTTGTCCTAAGTTTGAGTCTCACCATTAAAAACATCGACCACTATAAATTTTAGAAATTCACAACTTTACCAGGCTTGATGACACCATGAATTTTACAATGCCACTCCTTGTCCTAAATGTATCAGAGTATGTTTCTGAATCTACGTATAACGATTGTGTGGCAGACTGCTTAATTAATTTCTCCTCCTTTGATGGGTTAGGTGGCGACTAGTTTGTCGTACGAAAGTTGATATATATCAAAACATTAAAGACTAAAGCATACATATTTTTAGCTATGAGTTTTGTCTCTACTTCAAAGGAAAAGTCATTTCTTCATCCGTACAGAAAACCACAATAAACTCAGTGGCAGAAGGGATAGGAGTGATAACAGCTCAAGCAAGGGAAGATGCCACAGATGAAAGTGGTTTCACATTTGCATATTGCAACATAACAGGAACGGGAAACACTTATTTAGGAAGAGCTTGGAAAGAGAGGACTCGTGTTGTTTTTGCTTACACATACATGGGAACTCTCATCAACACAGAAGGATGGTCTGACAAAATGCATGGAAGCCAACCTAGAAAGTGAGCAAACTAACAAACAAAATCACACAAATGATCTTATAGTATATATTCGAAGGACGAGCCAACTAATTATTATTTTGATCGATGTTTAAATCTTTGAATACAGGAGTATGTACTACGGAGAGTACAAATGCAAGGGACCAGGCGCGACTTCGTCTGGTCGAGTGAAGTATGCCAGAATACTCTCGGACGTAGAAGCGAAGGCGTTTTTGAGTATGACATACATTCATGGAAACAAATGGCTTCTACCACCCCCTGATCTTTCTTTCTCCCCCTCTAAACCCAATATCTTCCATTAAAATTGAATCAAAATTACTTGAATTGAAAATGCTTAATCGAAATGGAAGTTAAATACTATCCCAAGCTGTGCCCAAAACCATAAAGTAATCACTATAGTGATAACTAGAAAAAGCAAAGGCCAAGGTAGCTAGGCTGTATGCTGTACCTAGAAAACTTTATCCCCATCATATTATTCACATAGAATGAATTTCCCTTTTTTTAGGTGATTGTTGCTGTTGTTTGTGTAAACTCTTTTTTGCTTTCCAACACTAATAGTTGTGGGTGGTGAAGTTTTGCAATACAACCTAATGTGGTTTTAAAATTCTTGTATTACCATTACATTGAGAAAGGTTACTTAATATGATCACTATGCACGCTTTGGTGCATTTGGATGGAGTGGAAACAATCAAGTCTTCAGCAACAACTATGGGGATTATTCTAATGGATGAGCATTTGGTCGCTACTTTGGTCTACAAAACTCTCCCTTTTTTGTTAATTGTTCCTTTTCTTTTATTGACCTTAAACTGGCACTTGTTCAATGTAACTCATTCGTTCGTATGTACTTTACATACCTACTTAATTATGAAGTATATTCTTTCAAAATAAAAAAAAATCAAATGTTGAAGCATAAATCTGTCAAATAACTTTTGTGTTACTCATCCATTGGGAGGCTAGATTATTATATATCATTCTACGAAGGCG

mRNA sequence

AACTACTACAAAGTCCTCTCCCTTCTCTTATTTTATCCCGAGTCCAAAAAACCGAAACACGAGGAGATTTTCGGGAGAGAATGTTTCGGCCGACCGTTGCTTCATCTTTTTCGGCGACGGTGGCGTTGGCATTGGCGATGATGTTCCAACTCATAATCCTAATAACTATTCCGTCCACCGTCCTCGTCGAAGCTCGCACGAAAACAATTCCGGCAGACTCGTCGAAGCTGGACGAATGGATCGCACACAACATGAGAGAATACAACGATCGTAAGATGAACGAGACAGGAATCAAGGCGCTGGACCGGCGGCTGGCTGAGGCGGAGGATTGCGTTCGATTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCTCGACCATCACTGAAGCGATCGAAAGCATTCCGTCAGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGAGGTGGAGTTTATAGAGAGAAAATTACGATCGATGCTTCCAAGCCGTTTGTGACTTTGTATGGTCAGAAAGGAAAACGGCCAGTGATAACATTCGACGGTACGGCGTCGGAGTTTGGTACCGTTAAGAGTGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTACGTTTGTGAATTCAGCTCCAATGCCAGAACTAGGAGGAACGGGAGGGCAAGCAGTGGCGATGAGGATATCTGGGGACAAGGCAGCATTCCATGAGTGTCATTTCATAGGCTTTCAAGACACTTTATGTGATGACAGAGGCAGACATTTTTTCAGGGATTGTTACATCCAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCTCTCTACTTGAAAACCACAATAAACTCAGTGGCAGAAGGGATAGGAGTGATAACAGCTCAAGCAAGGGAAGATGCCACAGATGAAAGTGGTTTCACATTTGCATATTGCAACATAACAGGAACGGGAAACACTTATTTAGGAAGAGCTTGGAAAGAGAGGACTCGTGTTGTTTTTGCTTACACATACATGGGAACTCTCATCAACACAGAAGGATGGTCTGACAAAATGCATGGAAGCCAACCTAGAAAGAGTATGTACTACGGAGAGTACAAATGCAAGGGACCAGGCGCGACTTCGTCTGGTCGAGTGAAGTATGCCAGAATACTCTCGGACGTAGAAGCGAAGGCGTTTTTGAGTATGACATACATTCATGGAAACAAATGGCTTCTACCACCCCCTGATCTTTCTTTCTCCCCCTCTAAACCCAATATCTTCCATTAAAATTGAATCAAAATTACTTGAATTGAAAATGCTTAATCGAAATGGAAGTTAAATACTATCCCAAGCTGTGCCCAAAACCATAAAGTAATCACTATAGTGATAACTAGAAAAAGCAAAGGCCAAGGTAGCTAGGCTGTATGCTGTACCTAGAAAACTTTATCCCCATCATATTATTCACATAGAATGAATTTCCCTTTTTTTAGGTGATTGTTGCTGTTGTTTGTGTAAACTCTTTTTTGCTTTCCAACACTAATAGTTGTGGGTGGTGAAGTTTTGCAATACAACCTAATGTGGTTTTAAAATTCTTGTATTACCATTACATTGAGAAAGGTTACTTAATATGATCACTATGCACGCTTTGGTGCATTTGGATGGAGTGGAAACAATCAAGTCTTCAGCAACAACTATGGGGATTATTCTAATGGATGAGCATTTGGTCGCTACTTTGGTCTACAAAACTCTCCCTTTTTTGTTAATTGTTCCTTTTCTTTTATTGACCTTAAACTGGCACTTGTTCAATGTAACTCATTCGTTCGTATGTACTTTACATACCTACTTAATTATGAAGTATATTCTTTCAAAATAAAAAAAAATCAAATGTTGAAGCATAAATCTGTCAAATAACTTTTGTGTTACTCATCCATTGGGAGGCTAGATTATTATATATCATTCTACGAAGGCG

Coding sequence (CDS)

ATGTTTCGGCCGACCGTTGCTTCATCTTTTTCGGCGACGGTGGCGTTGGCATTGGCGATGATGTTCCAACTCATAATCCTAATAACTATTCCGTCCACCGTCCTCGTCGAAGCTCGCACGAAAACAATTCCGGCAGACTCGTCGAAGCTGGACGAATGGATCGCACACAACATGAGAGAATACAACGATCGTAAGATGAACGAGACAGGAATCAAGGCGCTGGACCGGCGGCTGGCTGAGGCGGAGGATTGCGTTCGATTAATCACCGTTAGAAAAGACGGACGAGGAAATTTCTCGACCATCACTGAAGCGATCGAAAGCATTCCGTCAGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGAGGTGGAGTTTATAGAGAGAAAATTACGATCGATGCTTCCAAGCCGTTTGTGACTTTGTATGGTCAGAAAGGAAAACGGCCAGTGATAACATTCGACGGTACGGCGTCGGAGTTTGGTACCGTTAAGAGTGCGACGGTGGCGGTGGAATCGGATTATTTCGTTGCCGTCAATCTTACGTTTGTGAATTCAGCTCCAATGCCAGAACTAGGAGGAACGGGAGGGCAAGCAGTGGCGATGAGGATATCTGGGGACAAGGCAGCATTCCATGAGTGTCATTTCATAGGCTTTCAAGACACTTTATGTGATGACAGAGGCAGACATTTTTTCAGGGATTGTTACATCCAAGGCACTGTGGACTTCATCTTTGGAAATGGCAAATCTCTCTACTTGAAAACCACAATAAACTCAGTGGCAGAAGGGATAGGAGTGATAACAGCTCAAGCAAGGGAAGATGCCACAGATGAAAGTGGTTTCACATTTGCATATTGCAACATAACAGGAACGGGAAACACTTATTTAGGAAGAGCTTGGAAAGAGAGGACTCGTGTTGTTTTTGCTTACACATACATGGGAACTCTCATCAACACAGAAGGATGGTCTGACAAAATGCATGGAAGCCAACCTAGAAAGAGTATGTACTACGGAGAGTACAAATGCAAGGGACCAGGCGCGACTTCGTCTGGTCGAGTGAAGTATGCCAGAATACTCTCGGACGTAGAAGCGAAGGCGTTTTTGAGTATGACATACATTCATGGAAACAAATGGCTTCTACCACCCCCTGATCTTTCTTTCTCCCCCTCTAAACCCAATATCTTCCATTAA

Protein sequence

MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH
Homology
BLAST of IVF0000442 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 359.4 bits (921), Expect = 5.3e-98
Identity = 174/358 (48.60%), Postives = 238/358 (66.48%), Query Frame = 0

Query: 25  IILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAEDC 84
           I+L+ I S V+       IP +  ++++W   N                           
Sbjct: 12  ILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------------- 71

Query: 85  VRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQK 144
                V+++GRG+F TITEAI S+ +GN RRV++ IG GVY+EK+TID SKPF+TLYG  
Sbjct: 72  -----VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHP 131

Query: 145 GKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISG 204
              PV+TFDGTA+++GTV SAT+ V SDYF+AVN+   NSAPMP+    G QA++MRISG
Sbjct: 132 NAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISG 191

Query: 205 DKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGIGV 264
           +KAAF+ C F G+QDT+CDD G HFF+DCYI+GT DFIFG+G+SLYL T +N V +GI V
Sbjct: 192 NKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDGIRV 251

Query: 265 ITAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEGWSDK 324
           ITA A + A ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N  GW + 
Sbjct: 252 ITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQEN 311

Query: 325 MHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 382
               +  K+++YGEYKC G G+    RVKY + + D+EAK F+S+ YI G+ WLLPPP
Sbjct: 312 REAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPP 336

BLAST of IVF0000442 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 4.5e-97
Identity = 172/362 (47.51%), Postives = 248/362 (68.51%), Query Frame = 0

Query: 21  MFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAE 80
           M  +  L+   S V++   T  IP + +++ +W   N++ Y+ RK        LD  L  
Sbjct: 6   MSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRK------GTLDPALEA 65

Query: 81  AEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTL 140
           AE   ++ITV + G  NF T+ EAI+SIP+GN+ RV++ +  GVY EK+TID ++PF+TL
Sbjct: 66  AEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITL 125

Query: 141 YGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAM 200
            GQ G   V+T+ GTA+++GTV+SAT+ V ++YF A +LT  N+APMP+  G+ GQA+AM
Sbjct: 126 LGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQALAM 185

Query: 201 RISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAE 260
           RI+ DKAAF+ C F GFQDTLCDD+G HFF+DCYI+GT DFIFG G SLYL T +++V +
Sbjct: 186 RINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGD 245

Query: 261 GIGVITAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEG 320
           G+ VITAQ R+ AT+++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  G
Sbjct: 246 GLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSG 305

Query: 321 WSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLP 380
           W + ++     K+++YGEYKC GPG+    RV Y + +   E   FL++ YI G+ WLLP
Sbjct: 306 WRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLP 359

Query: 381 PP 382
           PP
Sbjct: 366 PP 359

BLAST of IVF0000442 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-92
Identity = 161/313 (51.44%), Postives = 222/313 (70.93%), Query Frame = 0

Query: 72  KALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITI 131
           K LD  L  AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G YREK+TI
Sbjct: 51  KGLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTI 110

Query: 132 DASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELG 191
           D +KPF+TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+  
Sbjct: 111 DRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGK 170

Query: 192 GTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYL 251
             G QA++MRISG+ AAF+ C F GFQDT+CDD G HFF+DCY++GT DFIFG+G S+YL
Sbjct: 171 TKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYL 230

Query: 252 KTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTY 311
            T ++ V +GI VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT 
Sbjct: 231 GTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTE 290

Query: 312 MGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTY 371
           M +++N  GW +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ Y
Sbjct: 291 MTSVVNPTGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGY 350

Query: 372 IHGNKWLLPPPDL 384
           I G+KWLLPPP L
Sbjct: 351 IQGSKWLLPPPAL 361

BLAST of IVF0000442 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 7.4e-92
Identity = 171/362 (47.24%), Postives = 241/362 (66.57%), Query Frame = 0

Query: 24  LIILITIPSTVLVEARTKT-IPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAE 83
           L+ L+ +  T +V A   T IP    ++ +W   N+     R       K LD  L  AE
Sbjct: 9   LLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALVAAE 68

Query: 84  DCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYG 143
              R+I V   G G F T+T+AI+S+P+GN +RV++ +  G Y+EK+TID +KPF+TL G
Sbjct: 69  AAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMG 128

Query: 144 QKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRI 203
           Q    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+    G QA++MRI
Sbjct: 129 QPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRI 188

Query: 204 SGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGI 263
           SG+ AAF+ C F GFQDT+CDD G HFF+DCY++GT DFIFG+G S+YL T ++ V +GI
Sbjct: 189 SGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGI 248

Query: 264 GVITAQAREDATDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTEGWS 323
            VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  GW 
Sbjct: 249 RVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQ 308

Query: 324 DKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 383
           +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ YI G+KWLLPPP
Sbjct: 309 ENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPP 361

BLAST of IVF0000442 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 9.6e-92
Identity = 161/357 (45.10%), Postives = 241/357 (67.51%), Query Frame = 0

Query: 26  ILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAEDCV 85
           +L+   S V++      IPAD +++ +W   N++ ++ R+        LD  L  AE   
Sbjct: 11  LLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRR------GTLDPELEAAEASR 70

Query: 86  RLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQKG 145
           R+I V ++G G+F TI  AI+SIP  N+ RV++ +  G+Y EK+T+D  +P+VTL G+ G
Sbjct: 71  RVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPG 130

Query: 146 KRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISGD 205
               +T+ GTA+++GTV+SAT+ V +  F+A NL  +N++PMP+  GT GQA+AMRI+GD
Sbjct: 131 AETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQALAMRINGD 190

Query: 206 KAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGIGVI 265
           KAAF+ C F GFQDTLCDDRG HFF++CYI+GT DFIFG G SLYL T +++V +G+ VI
Sbjct: 191 KAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDGLRVI 250

Query: 266 TAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKM 325
            A  R+  T+++G++F +C +TG G   YLGRAW    +VV++YT M +++N  GW +  
Sbjct: 251 AAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQENR 310

Query: 326 HGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 382
             +   K+++YGEY C GPG+  + RV + + + + EA  FL++ YI G+KWLLPPP
Sbjct: 311 VRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

BLAST of IVF0000442 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 9.0e-226
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           MFRPTVASSFSATVALALA+MFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 395
           EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

BLAST of IVF0000442 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 2.5e-215
Identity = 372/394 (94.42%), Postives = 382/394 (96.95%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           MFRPTVASSF ATVA A+ +M QLIILI IPSTV+VEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETGIKALDRRLAEAEDCV+LITVRKDGRGNFSTITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEG GVITAQAREDATDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGAT SGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 395
           EAKAFLSMTYIHGNKWLLPPPDLS SPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

BLAST of IVF0000442 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.2e-183
Identity = 320/387 (82.69%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M RP VASSF ATVAL LA++ QLI ++ IP+ ++VEARTK IPA+ S+LDEWIAHNMRE
Sbjct: 1   MSRPAVASSFPATVALVLAVV-QLITML-IPAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC RLITV++DG+GNFSTIT+AIESIPSGN  RVVVWI
Sbjct: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 388
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

BLAST of IVF0000442 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.2e-183
Identity = 318/387 (82.17%), Postives = 348/387 (89.92%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M RP VASSF ATVAL LA++   +I I IP+ ++VEARTKTIPA+ S+LDEWIAHNMRE
Sbjct: 1   MSRPAVASSFPATVALVLAVV--QLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y  RK NETG K LD RLA+AEDC RLITV++DG+GNFSTIT+AIESIPSGNR RVVVWI
Sbjct: 61  YRQRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 388
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

BLAST of IVF0000442 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.1e-182
Identity = 321/394 (81.47%), Postives = 353/394 (89.59%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M+RP VASS    VA+ALA++ QLII+  +P+ V+  ARTK IPA+ SKLDEWI +NM+E
Sbjct: 1   MYRPAVASSSPVAVAIALAVI-QLIII--LPAAVV--ARTKPIPAEPSKLDEWIENNMKE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y++RK N+TG+KALD+RLA AED VRLITVR DGRGNF+TIT AIESIP+GN  RV+VWI
Sbjct: 61  YHERKANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
            GG+YREKITIDASKPFVTLYGQ GK PVITFDGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 AGGIYREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSA MP++  +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG+GVITAQAREDATD+SGFTF YCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMG LINTEGWSDKMH  +PRKSMYYGEYKC GPGAT +GRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDE 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 395
           EA+AFLSMTYIHGNKWLLPPP LSFS S PN FH
Sbjct: 361 EARAFLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

BLAST of IVF0000442 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 790 bits (2041), Expect = 1.64e-288
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           MFRPTVASSFSATVALALA+MFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

BLAST of IVF0000442 vs. NCBI nr
Match: XP_004144054.1 (putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Csa_007174 [Cucumis sativus])

HSP 1 Score: 756 bits (1951), Expect = 8.59e-275
Identity = 372/394 (94.42%), Postives = 382/394 (96.95%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           MFRPTVASSF ATVA A+ +M QLIILI IPSTV+VEA TKTIPADSSKLDEWI HNM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           YNDRK NETGIKALDRRLAEAEDCV+LITVRKDGRGNFSTITEAI+SIPSGNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITIDASKPFVTLYGQKGKRP+ITFDGTASEFGTVKSATVAVESDYFVAVNLT
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFF+DCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKTTINSVAEG GVITAQAREDATDESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGAT SGRVKYARILSDV
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPPDLS SPSKPNIFH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

BLAST of IVF0000442 vs. NCBI nr
Match: XP_038880021.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 702 bits (1812), Expect = 1.17e-253
Identity = 351/394 (89.09%), Postives = 371/394 (94.16%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M RPTVASS  A VA++LA+ FQLIILI   S+ +VEARTK IPAD+SKLD+WIAHNM+E
Sbjct: 1   MSRPTVASSSPAAVAVSLAL-FQLIILII--SSAVVEARTKMIPADASKLDQWIAHNMKE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y +RK NETGIKALDRRLA+AED VRLITVRKDGRGNF+TIT+AIESIPSGNRRRVVVWI
Sbjct: 61  YEERKANETGIKALDRRLAKAEDHVRLITVRKDGRGNFTTITKAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGGVYREKITI+ASKPFVTLYGQKG+RPVITFDGTASEFGTVKSATVAVESDYFVAVNL 
Sbjct: 121 GGGVYREKITIEASKPFVTLYGQKGRRPVITFDGTASEFGTVKSATVAVESDYFVAVNLA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFF+DCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE+ATD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTIINSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKC GPGAT SGRVKYARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDE 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394
           EAKAFLSMTYIHGNKWLLPPP+LS   S+ N+FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPNLSLFHSRSNVFH 391

BLAST of IVF0000442 vs. NCBI nr
Match: XP_022987214.1 (putative pectinesterase 63 [Cucurbita maxima])

HSP 1 Score: 649 bits (1675), Expect = 8.94e-233
Identity = 320/387 (82.69%), Postives = 350/387 (90.44%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M RP VASSF ATVAL LA++ QLI ++ IP+ ++VEARTK IPA+ S+LDEWIAHNMRE
Sbjct: 1   MSRPAVASSFPATVALVLAVV-QLITML-IPAAIVVEARTKPIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y  RK  ETG KALDRRLA+AEDC RLITV++DG+GNFSTIT+AIESIPSGN  RVVVWI
Sbjct: 61  YRQRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGTV+SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TDESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDD 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

BLAST of IVF0000442 vs. NCBI nr
Match: XP_023515642.1 (putative pectinesterase 63 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649 bits (1675), Expect = 8.94e-233
Identity = 317/387 (81.91%), Postives = 348/387 (89.92%), Query Frame = 0

Query: 1   MFRPTVASSFSATVALALAMMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60
           M RP VASSF ATVAL LA++  + ILI  P+ ++VEARTKTIPA+ S+LDEWIAHNMRE
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILI--PAAIVVEARTKTIPAEPSQLDEWIAHNMRE 60

Query: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120
           Y  RK NETG K LD RLA+AEDC R+ITV++DG+GNFSTIT+AIESIPSGNR RVVVWI
Sbjct: 61  YRQRKANETGAKTLDPRLAKAEDCARIITVKQDGQGNFSTITQAIESIPSGNRERVVVWI 120

Query: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180
           GGG YREKITID+SKPFVTLYGQ GK PVI++DGTASEFGT +SATVAVESDYFVAVN+ 
Sbjct: 121 GGGTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVA 180

Query: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240
           FVNSAPMP+ G TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFF+DCYI+GTVD
Sbjct: 181 FVNSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVD 240

Query: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT INSVAEG GVITAQARE++TD+SGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360
           RTRVVFAYTYMGTLINT+GWSDKM G++PRKSMYYGEYKCKGPGA S+GR  Y RIL+D 
Sbjct: 301 RTRVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDY 360

Query: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSP 387
           EAKAFLSMTYIHGNKW+LPPP LS  P
Sbjct: 361 EAKAFLSMTYIHGNKWVLPPPRLSLLP 385

BLAST of IVF0000442 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 3.7e-99
Identity = 174/358 (48.60%), Postives = 238/358 (66.48%), Query Frame = 0

Query: 25  IILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAEDC 84
           I+L+ I S V+       IP +  ++++W   N                           
Sbjct: 12  ILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------------- 71

Query: 85  VRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQK 144
                V+++GRG+F TITEAI S+ +GN RRV++ IG GVY+EK+TID SKPF+TLYG  
Sbjct: 72  -----VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHP 131

Query: 145 GKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISG 204
              PV+TFDGTA+++GTV SAT+ V SDYF+AVN+   NSAPMP+    G QA++MRISG
Sbjct: 132 NAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISG 191

Query: 205 DKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGIGV 264
           +KAAF+ C F G+QDT+CDD G HFF+DCYI+GT DFIFG+G+SLYL T +N V +GI V
Sbjct: 192 NKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDGIRV 251

Query: 265 ITAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEGWSDK 324
           ITA A + A ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N  GW + 
Sbjct: 252 ITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQEN 311

Query: 325 MHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 382
               +  K+++YGEYKC G G+    RVKY + + D+EAK F+S+ YI G+ WLLPPP
Sbjct: 312 REAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPP 336

BLAST of IVF0000442 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 3.2e-98
Identity = 172/362 (47.51%), Postives = 248/362 (68.51%), Query Frame = 0

Query: 21  MFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAE 80
           M  +  L+   S V++   T  IP + +++ +W   N++ Y+ RK        LD  L  
Sbjct: 6   MSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRK------GTLDPALEA 65

Query: 81  AEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTL 140
           AE   ++ITV + G  NF T+ EAI+SIP+GN+ RV++ +  GVY EK+TID ++PF+TL
Sbjct: 66  AEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITL 125

Query: 141 YGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAM 200
            GQ G   V+T+ GTA+++GTV+SAT+ V ++YF A +LT  N+APMP+  G+ GQA+AM
Sbjct: 126 LGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQALAM 185

Query: 201 RISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAE 260
           RI+ DKAAF+ C F GFQDTLCDD+G HFF+DCYI+GT DFIFG G SLYL T +++V +
Sbjct: 186 RINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGD 245

Query: 261 GIGVITAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEG 320
           G+ VITAQ R+ AT+++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N  G
Sbjct: 246 GLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNPSG 305

Query: 321 WSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLP 380
           W + ++     K+++YGEYKC GPG+    RV Y + +   E   FL++ YI G+ WLLP
Sbjct: 306 WRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLP 359

Query: 381 PP 382
           PP
Sbjct: 366 PP 359

BLAST of IVF0000442 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 341.3 bits (874), Expect = 1.1e-93
Identity = 161/313 (51.44%), Postives = 222/313 (70.93%), Query Frame = 0

Query: 72  KALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITI 131
           K LD  L  AE   R+I V   G G F T+T+AI+S+P+GN +RV++ +  G YREK+TI
Sbjct: 51  KGLDPALVAAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTI 110

Query: 132 DASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELG 191
           D +KPF+TL GQ    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+  
Sbjct: 111 DRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGK 170

Query: 192 GTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYL 251
             G QA++MRISG+ AAF+ C F GFQDT+CDD G HFF+DCY++GT DFIFG+G S+YL
Sbjct: 171 TKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYL 230

Query: 252 KTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTY 311
            T ++ V +GI VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT 
Sbjct: 231 GTQLHVVGDGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTE 290

Query: 312 MGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTY 371
           M +++N  GW +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ Y
Sbjct: 291 MTSVVNPTGWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGY 350

Query: 372 IHGNKWLLPPPDL 384
           I G+KWLLPPP L
Sbjct: 351 IQGSKWLLPPPAL 361

BLAST of IVF0000442 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 5.2e-93
Identity = 171/362 (47.24%), Postives = 241/362 (66.57%), Query Frame = 0

Query: 24  LIILITIPSTVLVEARTKT-IPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAE 83
           L+ L+ +  T +V A   T IP    ++ +W   N+     R       K LD  L  AE
Sbjct: 9   LLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALVAAE 68

Query: 84  DCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYG 143
              R+I V   G G F T+T+AI+S+P+GN +RV++ +  G Y+EK+TID +KPF+TL G
Sbjct: 69  AAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMG 128

Query: 144 QKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRI 203
           Q    PVIT+DGTA+++GTV SA++ + SDYF+AVN+   N+AP P+    G QA++MRI
Sbjct: 129 QPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRI 188

Query: 204 SGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGI 263
           SG+ AAF+ C F GFQDT+CDD G HFF+DCY++GT DFIFG+G S+YL T ++ V +GI
Sbjct: 189 SGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDGI 248

Query: 264 GVITAQAREDATDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTYMGTLINTEGWS 323
            VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  GW 
Sbjct: 249 RVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQ 308

Query: 324 DKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 383
           +    +   K+++YGEYKC GPG+  + RV + + + D EA  FLS+ YI G+KWLLPPP
Sbjct: 309 ENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPP 361

BLAST of IVF0000442 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 6.8e-93
Identity = 161/357 (45.10%), Postives = 241/357 (67.51%), Query Frame = 0

Query: 26  ILITIPSTVLVEARTKTIPADSSKLDEWIAHNMREYNDRKMNETGIKALDRRLAEAEDCV 85
           +L+   S V++      IPAD +++ +W   N++ ++ R+        LD  L  AE   
Sbjct: 11  LLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRR------GTLDPELEAAEASR 70

Query: 86  RLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWIGGGVYREKITIDASKPFVTLYGQKG 145
           R+I V ++G G+F TI  AI+SIP  N+ RV++ +  G+Y EK+T+D  +P+VTL G+ G
Sbjct: 71  RVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPG 130

Query: 146 KRPVITFDGTASEFGTVKSATVAVESDYFVAVNLTFVNSAPMPELGGTGGQAVAMRISGD 205
               +T+ GTA+++GTV+SAT+ V +  F+A NL  +N++PMP+  GT GQA+AMRI+GD
Sbjct: 131 AETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQALAMRINGD 190

Query: 206 KAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVDFIFGNGKSLYLKTTINSVAEGIGVI 265
           KAAF+ C F GFQDTLCDDRG HFF++CYI+GT DFIFG G SLYL T +++V +G+ VI
Sbjct: 191 KAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDGLRVI 250

Query: 266 TAQAREDATDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTYMGTLINTEGWSDKM 325
            A  R+  T+++G++F +C +TG G   YLGRAW    +VV++YT M +++N  GW +  
Sbjct: 251 AAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQENR 310

Query: 326 HGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDVEAKAFLSMTYIHGNKWLLPPP 382
             +   K+++YGEY C GPG+  + RV + + + + EA  FL++ YI G+KWLLPPP
Sbjct: 311 VRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKF35.3e-9848.60Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
Q9LY174.5e-9747.51Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1[more]
Q9LY191.5e-9251.44Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q84WM77.4e-9247.24Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9LY189.6e-9245.10Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BRI59.0e-22699.75Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1[more]
A0A0A0M2992.5e-21594.42Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1[more]
A0A6J1JI924.2e-18382.69Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1[more]
A0A6J1HB454.2e-18382.17Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1[more]
A0A6J1D0K02.1e-18281.47Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_008450966.11.64e-28899.75PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
XP_004144054.18.59e-27594.42putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Cs... [more]
XP_038880021.11.17e-25389.09putative pectinesterase 63 [Benincasa hispida][more]
XP_022987214.18.94e-23382.69putative pectinesterase 63 [Cucurbita maxima][more]
XP_023515642.18.94e-23381.91putative pectinesterase 63 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G61680.13.7e-9948.60Pectin lyase-like superfamily protein [more]
AT5G07430.13.2e-9847.51Pectin lyase-like superfamily protein [more]
AT5G07410.11.1e-9351.44Pectin lyase-like superfamily protein [more]
AT1G69940.15.2e-9347.24Pectin lyase-like superfamily protein [more]
AT5G07420.16.8e-9345.10Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 83..380
e-value: 4.3E-103
score: 347.0
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 88..374
e-value: 4.8E-71
score: 238.9
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 21..382
NoneNo IPR availablePANTHERPTHR31321:SF30PECTINESTERASEcoord: 21..382
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 235..244
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 87..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000442.2IVF0000442.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity