IVF0000271 (gene) Melon (IVF77) v1

Overview
NameIVF0000271
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionExonuclease domain-containing protein
Locationchr02: 475294 .. 477738 (-)
RNA-Seq ExpressionIVF0000271
SyntenyIVF0000271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAACATAGCAAAAAATATTTTTAATAATGGAAGATTATTTAAAACTATATCAAATCGAATAAAATATATATGTAGTAATTTAACTTAAAGAAAAAGATTAGATTAATTTTAAATTAGGAAGGAAAATATAAAAAAGAAAAGAAAGGATCCGTAAGTTTAGCTTTGACTTATTTTTTTATTAATTTTTTCGGAAGAATAATTACAGAAGAAGCAACGAAGCTAAAGATCTCAGAAGCTGTTTTTTTCTTCTCTTCCGACCACAATGTCTCCCAAGAGCACCTCTCCATCTTCTTCCACGCCATTTACTCCCCTTCTTTTCCTCGCTTAACTTACGTTATTCCTTTGCTTTCGTTTTTAAATGCACCATATTCCTCCCTCAGGTTTTATTTCAGGCAAGTCCTTTTTTTTTTTTTTTTTTTTTTTCTGTCTCTCGCTTTGTAATAACATTCGAAGTTGGTTTAAATTGCCGAAAAGTGGGAAGGTGTTGTACTCTTTTCAAATCTGAAGGTAAATAGTTCTAATTTGCGTGAGAATGAAATGGGTCGATTGCTTTTTCTGGTATAACCCTTTTTTTTTTCAATCTAATGCAGCAGTGCTTCATTTGGACGTTTTTAAGTGCCACCGATCAATTGGTTTGCGTTGTTTGATTTTTTGTTTCTTTGATTTTTAGTAATGAATGTTTGGGTTTCTCTTTGTGGGGTATTGACGAAAGATTGGAGATGGCGGGAGTTGGGAGTGTTGTAGGCCAAATCCATTTGATTACAATGTGTTTGTTAAAATGTCTGAATGAGATCGTTGATTATTTTTTTTTCTTTTTTGTCTTTGACGTAATTGTGCTTGTCCTTTTGAGATCCATCATATGGAGCTTTTAACTTTTTTGTTTTGTTTATTATTTTCGTTATTCTGTTTGATAATCAATGTGGAGAATTTACAGTTGATTCCTTTTTTGTTTCTTGATCTCTGATATGGGCTTATACATTGTTTGTCACACCCCATTATCTACAGGTTGAAGAAAATTGCTTTCGGTGCTGGATTTTGTTGGGTTTTCTGTCTAGATTTCCTCTTGTTGTGTTGTAAATTTAAATTTTAGCAGCTCCAATGTGCTTTTCAATCTTCCGATTTCCAAAATTTGGAGTCCCTATTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGAAATCATGGTCATAGTTGTTGGTATGATCAACTTTGTTTTAGGATCTATAACCTTGAAGGAGGACAGAACAAGAGATGGACCAGAAAATCTATAACTACCAAAGCGGGGGGGAAAGCAAAGACCAACCCTAGCAGTAAACCCACCAATATTACGAATGAAATCTTGCAGGAATCGATGTCAGCAAGCTGTACAGTTAACGTTAATAAGTCTGAGAAAAGTGGAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCATTCACCCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAGATCCGCAGTGCTATATTACAAATTCAAGCATACATGGCATTACAAACAATATGGTCGCTAGTTGCGGTGTTCCAAGGTATCGAAACGTTCATAGTAGTCTCATTTCTCTTATATCTTCCATTCAGTTCACTGCTAGTTACAGTCATTTATCAATCATATATTTTTATACATATTCAAACGTTCCTTTTCTTGTTCATATAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCCGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGATGTACCCTTTTTACTCCACGAATTCAGCCGTTACTCCATTGATATTCCTCAAGATTGGCTATTTCTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGGTTAGTTTTCCCTTTCCACATATTATCGTGCTCTAGTTTCTCTGTGAATAAAAGGATTGGTGTGAGGTAATCTAATATTGTTGAGGGCAATATCGTGATCAGATTCGAAGATTACGAAAATATCATTAGAAGCACTTTGTGAGTATTACGGTATAGAAGTGGACGGTGAAGCACATCGAGCATTGCCAGATGTGATATCATTGTCTTTGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATGTCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAGAGTGATGAAGCTATGTTTTTGTTGTAATGTTCTTTACTAATTGTTGCAACTTTCTATTCATTCTTATTTGAATAACTTTGGGTCATTTTATTTACGGTCATCAGATCCTTCTTGATGTCCATATTCTCCAAAA

mRNA sequence

ATTTAACATAGCAAAAAATATTTTTAATAATGGAAGATTATTTAAAACTATATCAAATCGAATAAAATATATATGTAGTAATTTAACTTAAAGAAAAAGATTAGATTAATTTTAAATTAGGAAGGAAAATATAAAAAAGAAAAGAAAGGATCCGTAAGTTTAGCTTTGACTTATTTTTTTATTAATTTTTTCGGAAGAATAATTACAGAAGAAGCAACGAAGCTAAAGATCTCAGAAGCTGTTTTTTTCTTCTCTTCCGACCACAATGTCTCCCAAGAGCACCTCTCCATCTTCTTCCACGCCATTTACTCCCCTTCTTTTCCTCGCTTAACTTACGTTATTCCTTTGCTTTCGTTTTTAAATGCACCATATTCCTCCCTCAGGTTTTATTTCAGGTTGAAGAAAATTGCTTTCGGTGCTGGATTTTGTTGGGTTTTCTGTCTAGATTTCCTCTTGTTGTGTTGTAAATTTAAATTTTAGCAGCTCCAATGTGCTTTTCAATCTTCCGATTTCCAAAATTTGGAGTCCCTATTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGAAATCATGGTCATAGTTGTTGGTATGATCAACTTTGTTTTAGGATCTATAACCTTGAAGGAGGACAGAACAAGAGATGGACCAGAAAATCTATAACTACCAAAGCGGGGGGGAAAGCAAAGACCAACCCTAGCAGTAAACCCACCAATATTACGAATGAAATCTTGCAGGAATCGATGTCAGCAAGCTGTACAGTTAACGTTAATAAGTCTGAGAAAAGTGGAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCATTCACCCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAGATCCGCAGTGCTATATTACAAATTCAAGCATACATGGCATTACAAACAATATGGTCGCTAGTTGCGGTGTTCCAAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCCGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGATGTACCCTTTTTACTCCACGAATTCAGCCGTTACTCCATTGATATTCCTCAAGATTGGCTATTTCTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGATTCGAAGATTACGAAAATATCATTAGAAGCACTTTGTGAGTATTACGGTATAGAAGTGGACGGTGAAGCACATCGAGCATTGCCAGATGTGATATCATTGTCTTTGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATGTCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAGAGTGATGAAGCTATGTTTTTGTTGTAATGTTCTTTACTAATTGTTGCAACTTTCTATTCATTCTTATTTGAATAACTTTGGGTCATTTTATTTACGGTCATCAGATCCTTCTTGATGTCCATATTCTCCAAAA

Coding sequence (CDS)

ATGTGCTTTTCAATCTTCCGATTTCCAAAATTTGGAGTCCCTATTTTAGCTAACCTTTGGAGGGAAAACTTTCATCCCTTGAACAGAAATCATGGTCATAGTTGTTGGTATGATCAACTTTGTTTTAGGATCTATAACCTTGAAGGAGGACAGAACAAGAGATGGACCAGAAAATCTATAACTACCAAAGCGGGGGGGAAAGCAAAGACCAACCCTAGCAGTAAACCCACCAATATTACGAATGAAATCTTGCAGGAATCGATGTCAGCAAGCTGTACAGTTAACGTTAATAAGTCTGAGAAAAGTGGAACTCAGAAGCTTCAATATTGTGATATTCAACCAACAATTATTAAGAGCAAGGAATTCATTCACCCGGTGACAGTTATAACCTTTGACATTGAAACGACAGGTTTTAGTAGAAACCTCGATAGAATAGTGGAAATTGCATTTCAAGATCTTTCAGGAGGAGAAAATAGCACCTTTCAAACATTAATAGATCCGCAGTGCTATATTACAAATTCAAGCATACATGGCATTACAAACAATATGGTCGCTAGTTGCGGTGTTCCAAGGATGCAGGAACTTATTCCAATTATATTACAATTTGTCAAAAGCCGTCAGAAACCCGGTGGTTATGTATTATTGGTAGCTCATAATGCTCGTACCTTCGATGTACCCTTTTTACTCCACGAATTCAGCCGTTACTCCATTGATATTCCTCAAGATTGGCTATTTCTCGATACTATGACGTTAGCCCGTCAACTGATGAAGTTATCCGATTCGAAGATTACGAAAATATCATTAGAAGCACTTTGTGAGTATTACGGTATAGAAGTGGACGGTGAAGCACATCGAGCATTGCCAGATGTGATATCATTGTCTTTGATTCTTCAGAGGCTTACTTTCGACCTCAAGCTAGATGTCTCGGATCTCGTTGAAAGAGCTTTCACTCCTTTAGATCTGATTAATAAGAAGAAAAAATAG

Protein sequence

MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSITTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSKEFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGITNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIPQDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRLTFDLKLDVSDLVERAFTPLDLINKKKK
Homology
BLAST of IVF0000271 vs. ExPASy Swiss-Prot
Match: Q682U6 (Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DPD1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.7e-57
Identity = 134/323 (41.49%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SI +  +  +    +   E  H   +N   S     L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +T   G      SS             +  +  V     E+S T+   + ++Q  + + K
Sbjct: 61  STTTQGSRSNTKSS------------VLGGTVPVTRIIDEESRTKVQPFGNLQQRLAQDK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCY-ITNSSIHGI 180
           +    +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL++P    ITN+ IHGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDI 240
            N+MV    VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQDWLFLDTMTLARQLMKLSDSKI-TKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           P +WL LD++ LAR+ MK  +  +    SLEAL +YY +  +G+AHRAL DV+ LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLDVSDLVERAFTPLDL 322
           +LT DLKL +SDLV R  T  D+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of IVF0000271 vs. ExPASy Swiss-Prot
Match: Q8XJR3 (DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=polC PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.7e-13
Identity = 67/216 (31.02%), Postives = 106/216 (49.07%), Query Frame = 0

Query: 114 PTIIKSKEFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLIDPQCYIT 173
           P +I   E       + FDIETTGFS   D+I+EI    L  GE   +F T +DP+  I 
Sbjct: 421 PIVINGHEESFDDEYVVFDIETTGFSSKNDKIIEIGAVKLKDGEIVDSFSTFVDPKVNIP 480

Query: 174 N--SSIHGITNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLH 233
              + +  IT NMV   G P + E++P  ++FV       G  +LVAHNA  FDV F+  
Sbjct: 481 YKITELTSITQNMVN--GQPTIDEVLPKFMEFV-------GNSVLVAHNA-AFDVGFIKK 540

Query: 234 EFSRYSIDIPQDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDV 293
                   +      +DT+ LAR L       + K+ L  + ++ GI ++   HRA+ D 
Sbjct: 541 NLMDMGKTLKNP--VMDTVPLARYLY----PDLKKVKLNLVAKHLGISLENH-HRAVDDA 600

Query: 294 ISLSLIL----QRLTFDLKL-DVSDLVERAFTPLDL 322
            + + IL    +++  ++ + DV  L E+  + +D+
Sbjct: 601 KATAEILKFSFKKMKEEMDIHDVKTLNEKYLSNIDV 619

BLAST of IVF0000271 vs. ExPASy Swiss-Prot
Match: Q8RA32 (DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=polC PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.6e-10
Identity = 51/164 (31.10%), Postives = 85/164 (51.83%), Query Frame = 0

Query: 129 ITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLIDPQCYITN--SSIHGITNNMVA 188
           + FDIETTG S   D I+EI    +   +   TF+T ++PQ  I++  + + GI  +MV 
Sbjct: 389 VVFDIETTGLSNINDEIIEIGAVKIKNKKIVDTFETFVNPQIPISSFITKLTGIDESMVK 448

Query: 189 SCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIPQDWLF 248
               P ++E++P  L+F K         +LVAHNA  FDV F+  +  +  + +      
Sbjct: 449 D--APLIEEVLPKFLEFAKG-------AVLVAHNA-NFDVSFIKSKAKKLGLTVEN--TV 508

Query: 249 LDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPD 290
           LDT+ L+R L +     +    L+ L E++ +++    HRA+ D
Sbjct: 509 LDTLELSRHLYQ----DLKNYKLDTLAEFFEVKL-LHHHRAVED 535

BLAST of IVF0000271 vs. ExPASy Swiss-Prot
Match: Q8EQU6 (DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=polC PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-09
Identity = 55/177 (31.07%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 127 TVITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLIDPQCYITNSSIH--GITNNM 186
           T + FD+ETTG S   D I+E+A   + GGE    F++  +P   ++ ++I   GIT++M
Sbjct: 413 TFVVFDVETTGLSAVYDTIIELAAVKIKGGEIVDRFESFANPHHALSQTTIDLTGITDDM 472

Query: 187 VASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIPQDW 246
           V            P +   +K      G  +LVAHNA +FD+ FL   F R   +   + 
Sbjct: 473 VNDA---------PEVADVLKDFHTWMGNDILVAHNA-SFDMGFLNQGFQRIDYEKASNP 532

Query: 247 LFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 301
           + +DT+ LAR L+     ++    L  LC++  IE+  + HRA+ D  +   +L +L
Sbjct: 533 V-VDTLELARFLL----PELRNHRLNTLCKHLDIELT-QHHRAIYDAEATGYLLWKL 573

BLAST of IVF0000271 vs. ExPASy Swiss-Prot
Match: Q895K2 (DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=polC PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.0e-09
Identity = 54/180 (30.00%), Postives = 87/180 (48.33%), Query Frame = 0

Query: 129 ITFDIETTGFSRNLDRIVEIAFQDLSGGE-NSTFQTLIDPQCYITNSSIH--GITNNMVA 188
           + FDIETTGFS   D+I+EI    +  G+    + T ++P+  I    I   GI + MV 
Sbjct: 414 VVFDIETTGFSNKNDKIIEIGAVKIKEGKVVDKYSTFVNPERIIPEKIIELTGIHDYMVK 473

Query: 189 SCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIPQDWLF 248
               P+++E++P  + F++         +LVAHNA  FDV F+      + I        
Sbjct: 474 D--APKIEEVLPKFIDFIEDS-------ILVAHNA-NFDVSFIKKNCKDFGIHFNNP--I 533

Query: 249 LDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRLTFDLK 306
           LDT+ L + L      ++ +  L  + ++ GI +    HRA+ D  +   IL +   DLK
Sbjct: 534 LDTIPLCKFLY----PELKRYKLNVVAKHLGIPLLNH-HRAVEDAKTTGDILLKAFEDLK 576

BLAST of IVF0000271 vs. ExPASy TrEMBL
Match: A0A1S4DY73 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492132 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 4.4e-186
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI
Sbjct: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT
Sbjct: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP
Sbjct: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
           QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL
Sbjct: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 328
           TFDLKLDVSDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. ExPASy TrEMBL
Match: A0A0A0LVY3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 292/327 (89.30%), Postives = 308/327 (94.19%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQESM ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           N MV S  VPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 328
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. ExPASy TrEMBL
Match: A0A0N9LRH7 (Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.4e-163
Identity = 290/327 (88.69%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQES+ ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           N MV S  VP MQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 328
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. ExPASy TrEMBL
Match: A0A6J1HUD7 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467897 PE=4 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.3e-141
Identity = 256/327 (78.29%), Postives = 284/327 (86.85%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSI R PKFGVP LANLWRENFHPLNRN+GHS WYDQL FRIYNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKP+NI NEIL+ES+S SCTV++NKSEK GTQKLQ C+IQ TIIK K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           E   PVTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLI+P+CYI N+ +HGIT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           + MV+   VPRM+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR S+DIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF D + LARQLMK +DSKITKISL+AL EYY I + G AHRA+ DV +LSLILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 328
           TFDLKL + DL+ER+FT  DLINKKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKK 332

BLAST of IVF0000271 vs. ExPASy TrEMBL
Match: A0A6J1HDU6 (exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461962 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.3e-140
Identity = 251/326 (76.99%), Postives = 284/326 (87.12%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSI R PKFGVP LANLWRENFHPLNRN+GHS WYDQL FR YNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRTYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +TKAG KAKT+PSSKP+NI NEIL+ES+S SCTV++NKSEK+GTQKLQ C+IQ TI+K K
Sbjct: 66  STKAGRKAKTDPSSKPSNIRNEILEESVSTSCTVSINKSEKNGTQKLQCCNIQETIVKDK 125

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           E   PVTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLI+P+CYI N+ +HGIT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           + MV+   VPRM+ELIPI+LQF+KSRQKP GYVLLVAHNARTFDVPFLL EFSR S+DIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFIKSRQKPDGYVLLVAHNARTFDVPFLLKEFSRCSVDIP 245

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF D + LARQLMK +DSKITKISL+AL EYY I + G AHRA+ DV +LSLILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKK 327
           TFDLKL + DL+ER+FT  DLINKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKK 331

BLAST of IVF0000271 vs. NCBI nr
Match: XP_008450585.1 (PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo] >XP_016900923.1 PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 659 bits (1701), Expect = 5.90e-239
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI
Sbjct: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT
Sbjct: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP
Sbjct: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
           QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL
Sbjct: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327
           TFDLKLDVSDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. NCBI nr
Match: XP_004135590.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucumis sativus] >KGN66030.1 hypothetical protein Csa_007467 [Cucumis sativus])

HSP 1 Score: 588 bits (1515), Expect = 1.32e-210
Identity = 292/327 (89.30%), Postives = 308/327 (94.19%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQESM ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESMLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           N MV S  VPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NRMVDSPSVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. NCBI nr
Match: ALG62680.1 (exonuclease DPD1 [Cucumis sativus] >ALG62681.1 exonuclease DPD1 [Cucumis sativus])

HSP 1 Score: 584 bits (1505), Expect = 4.41e-209
Identity = 290/327 (88.69%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SIFRFPK+GVP+LANLWRENFHPLNRNHGH+CWYDQLCFRIYNLEGG+NKRWTRKSI
Sbjct: 1   MCLSIFRFPKYGVPVLANLWRENFHPLNRNHGHNCWYDQLCFRIYNLEGGRNKRWTRKSI 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKPTNI NEILQES+ ASCTVN+NKSEKS TQKLQYCDIQPTIIKSK
Sbjct: 61  TTKAGEKAKTNPSSKPTNIRNEILQESVLASCTVNINKSEKSETQKLQYCDIQPTIIKSK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           EF H VTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLI+PQCYITNS+IHGI+
Sbjct: 121 EFAHLVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLINPQCYITNSNIHGIS 180

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           N MV S  VP MQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLL EFSRYS+DIP
Sbjct: 181 NRMVDSPSVPSMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLSEFSRYSVDIP 240

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF DTMTLARQLMKLSDSKITKI+L+AL EYYGI++DG+AHRAL DV  LS ILQRL
Sbjct: 241 LNWLFFDTMTLARQLMKLSDSKITKITLQALGEYYGIKLDGKAHRALSDVRLLSSILQRL 300

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327
           TFDLKLD+SDLVERAFTPLDLINKKKK
Sbjct: 301 TFDLKLDISDLVERAFTPLDLINKKKK 327

BLAST of IVF0000271 vs. NCBI nr
Match: XP_022968752.1 (exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1 exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 510 bits (1314), Expect = 6.54e-180
Identity = 256/327 (78.29%), Postives = 284/327 (86.85%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSI R PKFGVP LANLWRENFHPLNRN+GHS WYDQL FRIYNLEGGQ+KRWTRKS+
Sbjct: 6   MCFSILRVPKFGVPTLANLWRENFHPLNRNYGHSSWYDQLGFRIYNLEGGQDKRWTRKSM 65

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAKTNPSSKP+NI NEIL+ES+S SCTV++NKSEK GTQKLQ C+IQ TIIK K
Sbjct: 66  TTKAGRKAKTNPSSKPSNIRNEILEESVSTSCTVSINKSEKYGTQKLQCCNIQETIIKDK 125

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           E   PVTVITFDIETTGFSR LDRIVEIA QDLSGGENSTFQTLI+P+CYI N+ +HGIT
Sbjct: 126 ELARPVTVITFDIETTGFSRELDRIVEIALQDLSGGENSTFQTLINPECYIPNAVVHGIT 185

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDIP 240
           + MV+   VPRM+ELIPI+LQFVKSRQKP GYVLLVAHNAR FDVPFLL EFSR S+DIP
Sbjct: 186 SRMVSRPDVPRMKELIPILLQFVKSRQKPDGYVLLVAHNARIFDVPFLLKEFSRCSVDIP 245

Query: 241 QDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQRL 300
            +WLF D + LARQLMK +DSKITKISL+AL EYY I + G AHRA+ DV +LSLILQRL
Sbjct: 246 LNWLFFDNILLARQLMKSTDSKITKISLQALREYYNIPLVGSAHRAMSDVRTLSLILQRL 305

Query: 301 TFDLKLDVSDLVERAFTPLDLINKKKK 327
           TFDLKL + DL+ER+FT  DLINKKKK
Sbjct: 306 TFDLKLGIPDLIERSFTTSDLINKKKK 332

BLAST of IVF0000271 vs. NCBI nr
Match: XP_038878496.1 (exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP_038878497.1 exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 508 bits (1308), Expect = 5.77e-179
Identity = 259/328 (78.96%), Postives = 285/328 (86.89%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MCFSI RFPKFGVP LANLWRENFHPLNRN  HS W+DQL FRIYNLEGGQNKRW RKS+
Sbjct: 6   MCFSILRFPKFGVPTLANLWRENFHPLNRNCYHSSWHDQLGFRIYNLEGGQNKRWIRKSM 65

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           TTKAG KAK NPSSKPTNI NEIL+ES+S SCTV++NKSEKS TQKLQYCDIQ  IIK+K
Sbjct: 66  TTKAGRKAKANPSSKPTNIRNEILEESISTSCTVSINKSEKSETQKLQYCDIQQLIIKNK 125

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCYITNSSIHGIT 180
           E   PVTVITFDIETTGFSRNLDRIVEIA QDLSGGENSTFQTLI+P+CYI NS +HGIT
Sbjct: 126 ELARPVTVITFDIETTGFSRNLDRIVEIALQDLSGGENSTFQTLINPECYIPNSVVHGIT 185

Query: 181 NNMVASCGVPRMQELIPIILQFVKSRQK--PGGYVLLVAHNARTFDVPFLLHEFSRYSID 240
           N+MV+   VPRM+ELIPI+LQFV+SRQK  P GYVLLVAHNARTFDVPFLL EFSR S D
Sbjct: 186 NHMVSRPDVPRMKELIPILLQFVESRQKQKPDGYVLLVAHNARTFDVPFLLKEFSRCSFD 245

Query: 241 IPQDWLFLDTMTLARQLMKLSDSKITKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           IP +W F D++ LARQLMK SDSKITKISL+AL EYY I++ G AHRA+ DV SLSLILQ
Sbjct: 246 IPPNWRFFDSIPLARQLMKSSDSKITKISLQALREYYDIQLVGSAHRAMSDVRSLSLILQ 305

Query: 301 RLTFDLKLDVSDLVERAFTPLDLINKKK 326
           RLTF+LKL +SDL+ER+FT  DLINKKK
Sbjct: 306 RLTFELKLGISDLIERSFTASDLINKKK 333

BLAST of IVF0000271 vs. TAIR 10
Match: AT5G26940.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 134/323 (41.49%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SI +  +  +    +   E  H   +N   S     L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +T   G      SS             +  +  V     E+S T+   + ++Q  + + K
Sbjct: 61  STTTQGSRSNTKSS------------VLGGTVPVTRIIDEESRTKVQPFGNLQQRLAQDK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCY-ITNSSIHGI 180
           +    +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL++P    ITN+ IHGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDI 240
            N+MV    VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQDWLFLDTMTLARQLMKLSDSKI-TKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           P +WL LD++ LAR+ MK  +  +    SLEAL +YY +  +G+AHRAL DV+ LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLDVSDLVERAFTPLDL 322
           +LT DLKL +SDLV R  T  D+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of IVF0000271 vs. TAIR 10
Match: AT5G26940.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 134/323 (41.49%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SI +  +  +    +   E  H   +N   S     L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +T   G      SS             +  +  V     E+S T+   + ++Q  + + K
Sbjct: 61  STTTQGSRSNTKSS------------VLGGTVPVTRIIDEESRTKVQPFGNLQQRLAQDK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCY-ITNSSIHGI 180
           +    +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL++P    ITN+ IHGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDI 240
            N+MV    VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQDWLFLDTMTLARQLMKLSDSKI-TKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           P +WL LD++ LAR+ MK  +  +    SLEAL +YY +  +G+AHRAL DV+ LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLDVSDLVERAFTPLDL 322
           +LT DLKL +SDLV R  T  D+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of IVF0000271 vs. TAIR 10
Match: AT5G26940.3 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 134/323 (41.49%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SI +  +  +    +   E  H   +N   S     L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +T   G      SS             +  +  V     E+S T+   + ++Q  + + K
Sbjct: 61  STTTQGSRSNTKSS------------VLGGTVPVTRIIDEESRTKVQPFGNLQQRLAQDK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCY-ITNSSIHGI 180
           +    +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL++P    ITN+ IHGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDI 240
            N+MV    VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQDWLFLDTMTLARQLMKLSDSKI-TKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           P +WL LD++ LAR+ MK  +  +    SLEAL +YY +  +G+AHRAL DV+ LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLDVSDLVERAFTPLDL 322
           +LT DLKL +SDLV R  T  D+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

BLAST of IVF0000271 vs. TAIR 10
Match: AT5G26940.4 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.2e-58
Identity = 134/323 (41.49%), Postives = 192/323 (59.44%), Query Frame = 0

Query: 1   MCFSIFRFPKFGVPILANLWRENFHPLNRNHGHSCWYDQLCFRIYNLEGGQNKRWTRKSI 60
           MC SI +  +  +    +   E  H   +N   S     L  R  +++G    RW R+++
Sbjct: 1   MCISISQVSRLRIHSFGSSCCERVHGWIKN---SSSLKLLDVRASSVDG--KARWIRRNV 60

Query: 61  TTKAGGKAKTNPSSKPTNITNEILQESMSASCTVNVNKSEKSGTQKLQYCDIQPTIIKSK 120
           +T   G      SS             +  +  V     E+S T+   + ++Q  + + K
Sbjct: 61  STTTQGSRSNTKSS------------VLGGTVPVTRIIDEESRTKVQPFGNLQQRLAQDK 120

Query: 121 EFIHPVTVITFDIETTGFSRNLDRIVEIAFQDLSGGENSTFQTLIDPQCY-ITNSSIHGI 180
           +    +TVI  D+ETTG  R  +RI+EIA QD++GG  STFQTL++P    ITN+ IHGI
Sbjct: 121 DLSKLLTVIVSDLETTGLHRKNERIIEIAAQDIAGGGYSTFQTLVNPGVVPITNAHIHGI 180

Query: 181 TNNMVASCGVPRMQELIPIILQFVKSRQKPGGYVLLVAHNARTFDVPFLLHEFSRYSIDI 240
            N+MV    VPRM+ELIPI L++V+SRQKPGGYV+LVAHN ++FD  FL++EF+R S +I
Sbjct: 181 RNDMVCRPEVPRMEELIPIFLRYVESRQKPGGYVMLVAHNGKSFDFQFLINEFNRCSYEI 240

Query: 241 PQDWLFLDTMTLARQLMKLSDSKI-TKISLEALCEYYGIEVDGEAHRALPDVISLSLILQ 300
           P +WL LD++ LAR+ MK  +  +    SLEAL +YY +  +G+AHRAL DV+ LS + Q
Sbjct: 241 PHNWLLLDSLPLARENMKSVEPTVKLSSSLEALADYYSLTREGDAHRALSDVLLLSKVFQ 300

Query: 301 RLTFDLKLDVSDLVERAFTPLDL 322
           +LT DLKL +SDLV R  T  D+
Sbjct: 301 KLTIDLKLSLSDLVLRCHTASDI 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q682U61.7e-5741.49Exonuclease DPD1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8XJR39.7e-1331.02DNA polymerase III PolC-type OS=Clostridium perfringens (strain 13 / Type A) OX=... [more]
Q8RA327.6e-1031.10DNA polymerase III PolC-type OS=Caldanaerobacter subterraneus subsp. tengcongens... [more]
Q8EQU61.0e-0931.07DNA polymerase III PolC-type OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP... [more]
Q895K25.0e-0930.00DNA polymerase III PolC-type OS=Clostridium tetani (strain Massachusetts / E88) ... [more]
Match NameE-valueIdentityDescription
A0A1S4DY734.4e-186100.00exonuclease DPD1, chloroplastic/mitochondrial OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0LVY31.6e-16489.30Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G569080... [more]
A0A0N9LRH72.4e-16388.69Exonuclease DPD1 OS=Cucumis sativus OX=3659 GN=DPD1 PE=2 SV=1[more]
A0A6J1HUD73.3e-14178.29exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HDU66.3e-14076.99exonuclease DPD1, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_008450585.15.90e-239100.00PREDICTED: exonuclease DPD1, chloroplastic/mitochondrial [Cucumis melo] >XP_0169... [more]
XP_004135590.11.32e-21089.30exonuclease DPD1, chloroplastic/mitochondrial [Cucumis sativus] >KGN66030.1 hypo... [more]
ALG62680.14.41e-20988.69exonuclease DPD1 [Cucumis sativus] >ALG62681.1 exonuclease DPD1 [Cucumis sativus... [more]
XP_022968752.16.54e-18078.29exonuclease DPD1, chloroplastic/mitochondrial [Cucurbita maxima] >XP_022968753.1... [more]
XP_038878496.15.77e-17978.96exonuclease DPD1, chloroplastic/mitochondrial isoform X1 [Benincasa hispida] >XP... [more]
Match NameE-valueIdentityDescription
AT5G26940.11.2e-5841.49Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.21.2e-5841.49Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.31.2e-5841.49Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G26940.41.2e-5841.49Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013520Exonuclease, RNase T/DNA polymerase IIISMARTSM00479exoiiienduscoord: 127..306
e-value: 5.1E-35
score: 132.3
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 130..290
e-value: 5.4E-22
score: 79.1
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 126..312
e-value: 4.6E-45
score: 155.8
IPR040393Three-prime repair exonuclease 1/2PANTHERPTHR13058THREE PRIME REPAIR EXONUCLEASE 1, 2coord: 2..323
NoneNo IPR availablePANTHERPTHR13058:SF23POLYMERASE III POLC-TYPE, PUTATIVE ISOFORM 1-RELATEDcoord: 2..323
NoneNo IPR availableCDDcd06127DEDDhcoord: 131..298
e-value: 7.8459E-42
score: 140.899
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 125..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000271.6IVF0000271.6mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000738 DNA catabolic process, exonucleolytic
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008296 3'-5'-exodeoxyribonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008408 3'-5' exonuclease activity