IVF0000182 (gene) Melon (IVF77) v1

Overview


Name	IVF0000182
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	beta-amyrin 11-oxidase-like
Location	chr04: 571134 .. 574286 (-)
RNA-Seq Expression	IVF0000182
Synteny	IVF0000182

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGATAAGTGTTTGTGTGATTATTGGAGTGGTTTTAGGGGTTTCTTTGTTGGTGTTCAGGAGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTTTTACAAAACATTCGTAGTTCAAGAAGATCCAATCTCTTTTATCCAATCTCTTCACTCAAGGTATCTCTTTCAAATCTCTTTCTTCTTAAATCGGAAGATTCTAAGCCGATAATCATTTCGATCGGTAGAAGCAATTTTGTTCTTATTTCTTAATTGTGGACTTTTTTATGCAGATATGGAAAAGGGGGAATATATAAAACTCACCTTTACGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATTTACTTAGACGAAGCAAAGTTCAAACAACATTATCCAAAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGAGTTCTCAAAGATGGATCACAAAATTGCATATAAATTAATGGCAGCACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGATGAGAGATCCTATTAAATTGTTGGAGGAAATTGGAAATCTCTTTTTCAAAGTCATTATAAAAGTTTTCTTAGGGTCTCAACTTGATGCCAAAACCATGGTTGAGCTTCATACATTGTACAAAGAAATGAGTTTTAGAGTGGTCATGTCCACTTTCCTTTATGACTTCCCTGGATTCACTTTTCGTCAAGCACTCAAGGTACACAATCTTTTGTTTTTTCTTCTTTTAATATATATATATATATATCAGTCCCATCTTTTCTTGACACCATCTTTGTTTTATCAGCACTCAACATCGTCTTTTTTTGAACAAAATTGTTGGTAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATGACAATCTCATTAGAGATTTGCTTCTTGGGGTATTTTTTGCTGGCTATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCCCATGTCTTCCAGAAGGCCAAGGTTAGGTTATTATATATTTTCATCCTTATACTTTCAATCAAAGCTAAGGTTTAATTAATTCTAAGATGTTTAATAAAATGAGTTAATTATTATTAATTAATTATTTACAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAGGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTTTCAAAGGTAATGTCAAGAATTGTTTTTAATTGTGGTAGATTTTAATTATTGTTGTCTTACTAATTTTCTATTGTTTTAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGCAAACATCAATGGTAAGTGTTTTCTTCTTATTATTATTATTATTTTATAGCATATAAGTATGTAAAAATTCATTCCCATTTTATATTATATACTATTGGTTCAAATTTGACATATTTGTTTTTTTAGGGAAAATAAAACTTTTATTAAAAAGCCACAAGAGAAGAAGAATGAAAGTTTTCCTTTAAGGAAGCATGAGAAATTATTATTATTAATACATAATATATGTTACTAATTATGTATTTAGATTGAAATTATTATTTTGTTGAGGATAAATAATTAACTAATTTATTTACTTTTTTCTATTGTTTTGTTTTTGTTTTCTGTTTTAGGTTACTTTATTCCGAAAGGATGGACGATACAAATTTGGAATATAGCTGTTCATATGGACCCTCAAATTTATTCAAATCCAAAAGAATTTAATCCTTCAAGATGGGAGGTACGTTTTATGATTATTTTAATCAACCATTAAAATAAATCCATATTAATTATTTAAAAAAATTAAGGTTATTTAAAATAAACTGCAGTGCTTTTGACTTTGATTCGTTTTGGTTATTAAACTTTTAAAAAGTAACCGTTTCCTTTTCATTTTAAGGAGAAAAAAACGTATAAATCTAAATAGTAATTTTTTCAAAATACAAGAACCAAAATTGAAGTCAAGACCAAAATGAATAAAAATCAAAGGGACGTTTGCAAAAACAGTAAAAATATTTATGATAATACGGCTAATGTCACTACATTTTTTAAATTGCAAAAGTAACAAATTTAAAAGCGAATAGTCCTTCGATAGCTATTTGATAACTCTTTGATAACCGTTTAATATATCTAATTACTTATCATTGTTGCAATATAAAAAAATGAAGTCTTGTGGACAAATTTTTTTTTTTCTAAATTTTTTTATCATCTGATGCAATTTTCCAAAATCAAAAGTATAAAAATTATGGTAACCATGATAGTATGTAAACCTTATAAATAAATAAAGATTTATTATTTGATACACAAGCACCACACGTGCACACACGAGCATGGGGTGAACATCCACGCACCTTCACACAAGCAAAATGTTCTCATACTAATGAATTCGATTTTGACCTGATTCATCGTTGTTGTTCCTCAGTAGTTTAGCACACGCACACATGTAAAAAATCTTAAGAATAGTGTTGGTGAATGAGAATCTTTTCTTGAGAAGGAAAGTAGAGAGAAATAATAACAAAATTAAGAATATTAATGTTAACAGTATTTGTAAAATGTGATGGGCAGAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGATTTTTCAAGGCTTGAGATTTCTATTCTACTCCATCATTTCATCCTCAATTACAAGTATGTATATGTATATATATATATATGCTTCTTCATTTTTCTTTCCTAAATTGATTTATTTTATTTTGCTAAAATAAAAAAGATATTGAATTGTTATTTTATTGGTGTATTTATTGTAGAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

mRNA sequence

ATGGAAATGATAAGTGTTTGTGTGATTATTGGAGTGGTTTTAGGGGTTTCTTTGTTGGTGTTCAGGAGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTTTTACAAAACATTCGTAGTTCAAGAAGATCCAATCTCTTTTATCCAATCTCTTCACTCAAGATATGGAAAAGGGGGAATATATAAAACTCACCTTTACGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATTTACTTAGACGAAGCAAAGTTCAAACAACATTATCCAAAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGAGTTCTCAAAGATGGATCACAAAATTGCATATAAATTAATGGCAGCACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGATGAGAGATCCTATTAAATTGTTGGAGGAAATTGGAAATCTCTTTTTCAAAGTCATTATAAAAGTTTTCTTAGGGTCTCAACTTGATGCCAAAACCATGGTTGAGCTTCATACATTGTACAAAGAAATGAGTTTTAGAGTGGTCATGTCCACTTTCCTTTATGACTTCCCTGGATTCACTTTTCGTCAAGCACTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATGACAATCTCATTAGAGATTTGCTTCTTGGGGTATTTTTTGCTGGCTATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCCCATGTCTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAGGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTTTCAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGCAAACATCAATGGTTACTTTATTCCGAAAGGATGGACGATACAAATTTGGAATATAGCTAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGATTTTTCAAGGCTTGAGATTTCTATTCTACTCCATCATTTCATCCTCAATTACAAAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

Coding sequence (CDS)

ATGGAAATGATAAGTGTTTGTGTGATTATTGGAGTGGTTTTAGGGGTTTCTTTGTTGGTGTTCAGGAGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAGAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTTTTACAAAACATTCGTAGTTCAAGAAGATCCAATCTCTTTTATCCAATCTCTTCACTCAAGATATGGAAAAGGGGGAATATATAAAACTCACCTTTACGGAAAGCCAACAGTGATTGCCACAGATCCAGAAATATGTCGACGGATTTACTTAGACGAAGCAAAGTTCAAACAACATTATCCAAAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGAGTTCTCAAAGATGGATCACAAAATTGCATATAAATTAATGGCAGCACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGATGAGAGATCCTATTAAATTGTTGGAGGAAATTGGAAATCTCTTTTTCAAAGTCATTATAAAAGTTTTCTTAGGGTCTCAACTTGATGCCAAAACCATGGTTGAGCTTCATACATTGTACAAAGAAATGAGTTTTAGAGTGGTCATGTCCACTTTCCTTTATGACTTCCCTGGATTCACTTTTCGTCAAGCACTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATGACAATCTCATTAGAGATTTGCTTCTTGGGGTATTTTTTGCTGGCTATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCCCATGTCTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAGGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTTTCAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGCAAACATCAATGGTTACTTTATTCCGAAAGGATGGACGATACAAATTTGGAATATAGCTAATTATACACCAAAGCCAGGAACCTTCATCCCTTTTGGACTTGGAAGTAGGTTTTGTCCTGGAAGTGATTTTTCAAGGCTTGAGATTTCTATTCTACTCCATCATTTCATCCTCAATTACAAAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

Protein sequence

MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIANYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRPENCLCKIIRLS

Homology

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BLAST of IVF0000182 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 213/501 (42.51%), Postives = 307/501 (61.28%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLL---VFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSF 60
           ME+  VC+    +L   +      R LN  +Y VKL  R    LPPGD+GWP+IG   SF
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKL-RRKEHPLPPGDMGWPLIGDLLSF 60

Query: 61  YKTFVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYP 120
            K F     P SFI +L  +YG+ GIYKTHL+G P++I  +P++CRR+  D+  FK  YP
Sbjct: 61  IKDF-SSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYP 120

Query: 121 KSVKILEGSSG--EFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMR 180
           KS+K L       + S  +H++  +L+ +P+ G + L+ Y++ +EE++   LEE SS M+
Sbjct: 121 KSIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSS-MK 180

Query: 181 DPIKLLEEIGNLFFKVIIKVFLGS---QLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGF 240
            P++LL+E+  + FK I+ VF+GS    +  K       LY  M F + ++      PGF
Sbjct: 181 HPVELLKEMKKVSFKAIVHVFMGSSNQDIIKKIGSSFTDLYNGM-FSIPINV-----PGF 240

Query: 241 TFRQALKARKKIENIIQGVVEEKRRRFEN--DKTSEVQCQVDKMVVAIDENGAKLYNDNL 300
           TF +AL+ARKK+  I+Q VV+E+R   EN   + S+ +  +D ++   DENG KL  D  
Sbjct: 241 TFHKALEARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKL-EDED 300

Query: 301 IRDLLLGVFFAGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEI 360
           I DLL+G+ FAG+ + A +  W++ +++Q+PH+ +KAKEEQE I R R S+QK L+L EI
Sbjct: 301 ISDLLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEI 360

Query: 361 KQMKYLSKVINEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA---------- 420
           KQM YLS+VI+E LR   I    FREA  D NINGY IPKGW + IW  A          
Sbjct: 361 KQMVYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPN 420

Query: 421 ----------NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESY 471
                     +Y  K GTF+PFG GSR CPG+D ++LEISI LH+F+ NY++E +N E +
Sbjct: 421 PEEFNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECH 480

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BLAST of IVF0000182 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.8e-92
Identity = 202/482 (41.91%), Postives = 286/482 (59.34%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG + +  LPPGDLGWPVIG+ +SF + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEG--SS 131
           FIQS  +RYG+ GIYK H++G P V+ T PE CRR+  D+  F   +PKS   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNL 191
              S  +HK   +L +AP+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENI 251
            FKVI+ +FL S+ +   M  L   Y  +++ V       + PGF + +ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYGV--RAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG ++ +D  I DLLL    AG+ +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YLS+VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDANINGYFIPKGWTIQIW--NI------------------ANYTPKPG 431
           T + T FREAK+D  ++GY IPKGW +  W  N+                    YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 469
           TF+PFGLGS  CPG+D ++LEISI LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

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BLAST of IVF0000182 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 8.2e-90
Identity = 177/492 (35.98%), Postives = 291/492 (59.15%), Query Frame = 0


Query: 3   MISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFV 62
           +++  V+ GVVL  +  V RR ++   +  LG      LPPG++GWP++GS ++F + F 
Sbjct: 19  VLAAAVVAGVVLVDA--VVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF- 78

Query: 63  VQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKI 122
              +P +FI S   R+G+ G+Y+T ++  PT++A  PE C+++ +D+  F   +PK+   
Sbjct: 79  KSGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 138

Query: 123 LEG--SSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 182
           L G  S    S  DH+   KL AAP+NG + L+ Y+ FI++ +   L  WSS     ++ 
Sbjct: 139 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 198

Query: 183 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 242
           L E+  + FK+I+++F+    DA TM  L   Y ++++   M     + PGF + +AL+A
Sbjct: 199 LTELRRMTFKIIVQIFMSGADDA-TMEALERSYTDLNYG--MRAMAINLPGFAYYRALRA 258

Query: 243 RKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVF 302
           R+K+ +++QGV++ +R    +  K S     +D+++ A DE G +L +D ++ D+L+   
Sbjct: 259 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIV-DVLIMYL 318

Query: 303 FAGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKV 362
            AG+ +      WA + + +NP +F +AK EQE I+R  P+ Q GLTL + K+M +LS+V
Sbjct: 319 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 378

Query: 363 INEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIW-----------------NIANY 422
           ++E LR   I+  +FR+A  D  +NGY IPKGW +Q+W                 N + +
Sbjct: 379 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 438

Query: 423 ---TPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP 471
               PK GTF+PFGLG+R CPG+D ++LEIS+ LHHF+L YK++  N +  +  +P PRP
Sbjct: 439 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRP 498

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BLAST of IVF0000182 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 5.0e-87
Identity = 177/496 (35.69%), Postives = 281/496 (56.65%), Query Frame = 0


Query: 5   SVCVIIGVVLGVSLLVFRRLNELWY-LVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVV 64
           +V  ++  +  V+L    R    WY    LG      LPPGD+GWP++G  ++F + F  
Sbjct: 8   AVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAF-K 67

Query: 65  QEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKIL 124
              P SFI S   R+G+ G+Y+  ++  PT++AT PE C+++ +D+  F   +PK+   L
Sbjct: 68  SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVAL 127

Query: 125 EGSSGEFSKM---DHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRD-PIK 184
            G    F  M   +H+   KL AAP+NG + L++Y+ FI++ +   L  WS    D   +
Sbjct: 128 IGPK-SFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFE 187

Query: 185 LLEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALK 244
            L E+  + F++I+++F+G   D +T  EL   Y E+++   M     D PGF + +A++
Sbjct: 188 FLTELRRMTFRIIVQIFMGG-ADERTAAELERTYTELNYG--MRAMAIDLPGFAYHKAIR 247

Query: 245 ARKKIENIIQGVVEEKRRRFENDKTSEVQC----QVDKMVVAIDENGAKLYNDNLIRDLL 304
           AR+++   +Q V++E+R R    KT+         +D+++   DE G +L +D +I D+L
Sbjct: 248 ARRRLVAALQRVLDERRAR--GGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEII-DVL 307

Query: 305 LGVFFAGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKY 364
           +    AG+ +      WA + + +NP +  KAK EQE+I+R  P  QKGLTL + ++M Y
Sbjct: 308 VMYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAY 367

Query: 365 LSKVINEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIANY------------- 424
           LS+V++E LR   I+  +FR+A  D  +NGY IPKGW +Q+W  + +             
Sbjct: 368 LSQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFD 427

Query: 425 -------TPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMP 471
                   P+ GTF+PFGLG+R CPG+D ++LEIS+ LHHF+L YK+   N    +  +P
Sbjct: 428 PSRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLP 487

[Close]

BLAST of IVF0000182 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.6e-85
Identity = 183/476 (38.45%), Postives = 269/476 (56.51%), Query Frame = 0


Query: 15  GVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQSL 74
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  SF + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 75  HSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEG--SSGEFSK 134
             RYG  GIYK H++G P++I T  + CRR+  D+  FK  +P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 135 MDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVI 194
            +HK   +L AAP+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 195 IKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENIIQGVV 254
           + +FL S+ +   M  L   Y  +++ V       + PGF + +ALKARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYGV--RAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 255 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFW 314
            E+R + + +  S  +  +D ++   DE+G K  +D  I D+LL    AG+ +      W
Sbjct: 261 TERRNQRKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMW 320

Query: 315 ALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRNTITPT 374
           A + + ++P V Q+AK EQE I++ RP  QKGL+L E ++M++LS+V++E LR  T + T
Sbjct: 321 ATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLT 380

Query: 375 NFREAKTDANINGYFIPKGWTIQIWN---------------------IANYTPKPGTFIP 434
            FREAKTD  +NGY IPKGW +  W                         + PK G F+P
Sbjct: 381 AFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLP 440

Query: 435 FGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 467
           FG GS  CPG+D ++LEISI LHHF+L Y+++  N E  +  +P  RP +NCL +I
Sbjct: 441 FGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of IVF0000182 vs. ExPASy TrEMBL
Match: A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.3e-236
Identity = 414/490 (84.49%), Postives = 442/490 (90.20%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGV LLV RRLNELWYLVKLGGRAYKSLPPGDLGWPVIG SFS YKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           F+VQEDPISFIQSLHSRYGKGG+YKTHLYG PTVI T+PEICRRIYLDEA FKQHYPKSV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSG+FS MDHKIAYK+MA+PMNG EVLSNYVDFIEEVIAKGLEEWSSMMR+PIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           ++EIG LFFKVI K+FLGSQLDAKTMVELHTLYKE+SF +VMSTF YDFPGFTF Q LKA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY++N IRDL+LG+FF
Sbjct: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVI 360
           AG+STPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYL+K+I
Sbjct: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360

Query: 361 NEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA-------------------- 420
           NEVLRRNTIT TNFRE KTD NING+FIPKGWT+QIWNIA                    
Sbjct: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRPE 471
           NYTPKPG FIPFGLGSRFCPGS+ ++LEI+ILLHHFILNYKMEGVNLES   QMPSPRPE
Sbjct: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPE 480

[Close]

BLAST of IVF0000182 vs. ExPASy TrEMBL
Match: A0A1S3CRX8 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.5e-230
Identity = 405/459 (88.24%), Postives = 425/459 (92.59%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCV IGVVLGVSLLVFRRLNELWYLVKLGG+AYKSLPPGDLGWPVIGSSFS YKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           F+VQEDPISFIQSLHSRYG+GGIYKTHLYGKP VIATD EICRRIYLDEAKFKQH P+SV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSG+FSKMDHKIAYK++A PMNGSEVLSNYVDFIEEVIAKGLEEWSSM+R+PIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           L+EIG+LFFKVI KVFLGS+LDAKTMVELH LYKE+SF +VMSTF YDFPGFTF QALKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYN+NLIRDLLLGV F
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVI 360
           AG+STPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYL+KVI
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360

Query: 361 NEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA-------------------- 420
           NEVLRRNTITPTNFREAKTD NINGYFIPKGWTIQIWNIA                    
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILN 440
           NYTPKPGTFIPFGLGSRFCPGS+ +RLEI+ILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

[Close]

BLAST of IVF0000182 vs. ExPASy TrEMBL
Match: A0A5D3E6A2 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004200 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.7e-183
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT
Sbjct: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV
Sbjct: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL
Sbjct: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA
Sbjct: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 329
           AGYSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328

[Close]

BLAST of IVF0000182 vs. ExPASy TrEMBL
Match: A0A5A7T6J1 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00700 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 6.6e-183
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT
Sbjct: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV
Sbjct: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL
Sbjct: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA
Sbjct: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYN+NLIRDLLLGVFF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 329
           AGYSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328

[Close]

BLAST of IVF0000182 vs. ExPASy TrEMBL
Match: A0A5A7T517 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00710 PE=4 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 1.2e-173
Identity = 309/317 (97.48%), Postives = 314/317 (99.05%), Query Frame = 0


Query: 13  VLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQ 72
           ++GVSLLVFRRLNELWYLVK GGRAYKSLPPGDLGWPVIGSSFS YKTFVVQEDPISFIQ
Sbjct: 1   MVGVSLLVFRRLNELWYLVKSGGRAYKSLPPGDLGWPVIGSSFSSYKTFVVQEDPISFIQ 60

Query: 73  SLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEGSSGEFSK 132
           SLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEGSSGEFSK
Sbjct: 61  SLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEGSSGEFSK 120

Query: 133 MDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVI 192
           MDHKIAYK+MAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVI
Sbjct: 121 MDHKIAYKVMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVI 180

Query: 193 IKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENIIQGVV 252
           IKVFLGSQLDAKTMVELHTLYKEMSF VVMSTFLYDFPGFTFRQALKARKKIENIIQGVV
Sbjct: 181 IKVFLGSQLDAKTMVELHTLYKEMSFGVVMSTFLYDFPGFTFRQALKARKKIENIIQGVV 240

Query: 253 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFW 312
           EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYN+NLIRDLLLGVFFAGYSTPAIAAFW
Sbjct: 241 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVFFAGYSTPAIAAFW 300

Query: 313 ALLHISQNPHVFQKAKE 330
           ALLHISQNPHVFQKAK+
Sbjct: 301 ALLHISQNPHVFQKAKK 317

[Close]

BLAST of IVF0000182 vs. NCBI nr
Match: XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])

HSP 1 Score: 825 bits (2132), Expect = 1.70e-299
Identity = 414/490 (84.49%), Postives = 442/490 (90.20%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGV LLV RRLNELWYLVKLGGRAYKSLPPGDLGWPVIG SFS YKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           F+VQEDPISFIQSLHSRYGKGG+YKTHLYG PTVI T+PEICRRIYLDEA FKQHYPKSV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSG+FS MDHKIAYK+MA+PMNG EVLSNYVDFIEEVIAKGLEEWSSMMR+PIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           ++EIG LFFKVI K+FLGSQLDAKTMVELHTLYKE+SF +VMSTF YDFPGFTF Q LKA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY++N IRDL+LG+FF
Sbjct: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVI 360
           AG+STPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYL+K+I
Sbjct: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360

Query: 361 NEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA-------------------- 420
           NEVLRRNTIT TNFRE KTD NING+FIPKGWT+QIWNIA                    
Sbjct: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRPE 470
           NYTPKPG FIPFGLGSRFCPGS+ ++LEI+ILLHHFILNYKMEGVNLES   QMPSPRPE
Sbjct: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPE 480

[Close]

BLAST of IVF0000182 vs. NCBI nr
Match: XP_008466645.1 (PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo])

HSP 1 Score: 805 bits (2080), Expect = 4.59e-292
Identity = 405/459 (88.24%), Postives = 425/459 (92.59%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCV IGVVLGVSLLVFRRLNELWYLVKLGG+AYKSLPPGDLGWPVIGSSFS YKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           F+VQEDPISFIQSLHSRYG+GGIYKTHLYGKP VIATD EICRRIYLDEAKFKQH P+SV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSG+FSKMDHKIAYK++A PMNGSEVLSNYVDFIEEVIAKGLEEWSSM+R+PIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           L+EIG+LFFKVI KVFLGS+LDAKTMVELH LYKE+SF +VMSTF YDFPGFTF QALKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYN+NLIRDLLLGV F
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVI 360
           AG+STPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYL+KVI
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360

Query: 361 NEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA-------------------- 420
           NEVLRRNTITPTNFREAKTD NINGYFIPKGWTIQIWNIA                    
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILN 439
           NYTPKPGTFIPFGLGSRFCPGS+ +RLEI+ILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

[Close]

BLAST of IVF0000182 vs. NCBI nr
Match: TYK31140.1 (beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 651 bits (1680), Expect = 3.60e-233
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT
Sbjct: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV
Sbjct: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL
Sbjct: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA
Sbjct: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328
           AGYSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328

[Close]

BLAST of IVF0000182 vs. NCBI nr
Match: KAA0038543.1 (beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 649 bits (1675), Expect = 2.08e-232
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT
Sbjct: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV
Sbjct: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL
Sbjct: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA
Sbjct: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYN+NLIRDLLLGVFF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVFF 300

Query: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328
           AGYSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 301 AGYSTPAIAAFWALLHISQNPHVFQKAK 328

[Close]

BLAST of IVF0000182 vs. NCBI nr
Match: XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 653 bits (1685), Expect = 1.89e-231
Identity = 322/493 (65.31%), Postives = 400/493 (81.14%), Query Frame = 0


Query: 1   MEMISVCVIIGVVLGVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKT 60
           ME+++VCVIIGVVLG  + V RR NE+WYL KLG +  KSLPPGDLGWP +GS+FSFYK 
Sbjct: 1   MELMNVCVIIGVVLGFYVFV-RRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKA 60

Query: 61  FVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSV 120
           F V+ DP +FI +L  RYG+ G+YK+HLYG PT+I T+PEICRRIYLD+ +F+ +YPKSV
Sbjct: 61  FKVEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSV 120

Query: 121 KILEGSSGEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKL 180
           KILE ++G+FSK+DHK  YK+MA+PMNGSEVLS +V+FIE+ + KGLEEWSSM R+PI+L
Sbjct: 121 KILE-TNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIEL 180

Query: 181 LEEIGNLFFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKA 240
           ++EIG L FK+I+ +FLG+++D + M ELHTLYKE+   V+MS   YD PGFT+R+ALKA
Sbjct: 181 VDEIGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDK-TSEV-QCQVDKMVVAIDENGAKLYNDNLIRDLLLGV 300
           RKKIE I+  V+E+KR+RFE D  T+EV  CQVDK++VA +ENG+K YN++ I DL+LG+
Sbjct: 241 RKKIEKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGI 300

Query: 301 FFAGYSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSK 360
           FFAG++TPAIAA WALLHISQNPH+FQ AKEEQESIIRQRPS QKGLT  EIKQMKYL+K
Sbjct: 301 FFAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTK 360

Query: 361 VINEVLRRNTITPTNFREAKTDANINGYFIPKGWTIQIWNIA------------------ 420
            INEVLRRNT+ PTNFR+A+T  NINGY IPKGWT+QIW++A                  
Sbjct: 361 FINEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSR 420

Query: 421 --NYTPKPGTFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPR 470
             NYTPKPG FIPFGLGSRFCPGS+ ++LEI+ILLHHFILNYKME V+    +T +PSP+
Sbjct: 421 WDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPK 480

[Close]

BLAST of IVF0000182 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-93
Identity = 202/482 (41.91%), Postives = 286/482 (59.34%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG + +  LPPGDLGWPVIG+ +SF + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEG--SS 131
           FIQS  +RYG+ GIYK H++G P V+ T PE CRR+  D+  F   +PKS   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNL 191
              S  +HK   +L +AP+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENI 251
            FKVI+ +FL S+ +   M  L   Y  +++ V       + PGF + +ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYGV--RAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG ++ +D  I DLLL    AG+ +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YLS+VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDANINGYFIPKGWTIQIW--NI------------------ANYTPKPG 431
           T + T FREAK+D  ++GY IPKGW +  W  N+                    YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 469
           TF+PFGLGS  CPG+D ++LEISI LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

[Close]

BLAST of IVF0000182 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-93
Identity = 202/482 (41.91%), Postives = 286/482 (59.34%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG + +  LPPGDLGWPVIG+ +SF + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEG--SS 131
           FIQS  +RYG+ GIYK H++G P V+ T PE CRR+  D+  F   +PKS   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GEFSKMDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNL 191
              S  +HK   +L +AP+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVIIKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENI 251
            FKVI+ +FL S+ +   M  L   Y  +++ V       + PGF + +ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYGV--RAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG ++ +D  I DLLL    AG+ +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YLS+VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDANINGYFIPKGWTIQIW--NI------------------ANYTPKPG 431
           T + T FREAK+D  ++GY IPKGW +  W  N+                    YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 469
           TF+PFGLGS  CPG+D ++LEISI LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

[Close]

BLAST of IVF0000182 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-86
Identity = 183/476 (38.45%), Postives = 269/476 (56.51%), Query Frame = 0


Query: 15  GVSLLVFRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQSL 74
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  SF + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 75  HSRYGKGGIYKTHLYGKPTVIATDPEICRRIYLDEAKFKQHYPKSVKILEG--SSGEFSK 134
             RYG  GIYK H++G P++I T  + CRR+  D+  FK  +P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 135 MDHKIAYKLMAAPMNGSEVLSNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVI 194
            +HK   +L AAP+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 195 IKVFLGSQLDAKTMVELHTLYKEMSFRVVMSTFLYDFPGFTFRQALKARKKIENIIQGVV 254
           + +FL S+ +   M  L   Y  +++ V       + PGF + +ALKARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYGV--RAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 255 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFW 314
            E+R + + +  S  +  +D ++   DE+G K  +D  I D+LL    AG+ +      W
Sbjct: 261 TERRNQRKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMW 320

Query: 315 ALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRNTITPT 374
           A + + ++P V Q+AK EQE I++ RP  QKGL+L E ++M++LS+V++E LR  T + T
Sbjct: 321 ATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLT 380

Query: 375 NFREAKTDANINGYFIPKGWTIQIWN---------------------IANYTPKPGTFIP 434
            FREAKTD  +NGY IPKGW +  W                         + PK G F+P
Sbjct: 381 AFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLP 440

Query: 435 FGLGSRFCPGSDFSRLEISILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 467
           FG GS  CPG+D ++LEISI LHHF+L Y+++  N E  +  +P  RP +NCL +I
Sbjct: 441 FGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of IVF0000182 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 183.7 bits (465), Expect = 3.4e-46
Identity = 132/456 (28.95%), Postives = 203/456 (44.52%), Query Frame = 0


Query: 35  GRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTV 94
           G +   LPPG +GWP +G +F  Y      +DP  F QS   RY  G ++KTH+ G P V
Sbjct: 30  GSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRY--GSVFKTHVLGCPCV 89

Query: 95  IATDPEICRRIYLDEAK-FKQHYPKSVKILEGSSG-EFSKMDHKIAYKLMAAPMNGSEVL 154
           + + PE  + + + ++  FK  +P S + + G     F + D+    + +       E +
Sbjct: 90  MISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESI 149

Query: 155 SNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVIIKVFLGSQLDAKTMVELHTL 214
            N V  IE +    L  W   M   I   +E+    F V +    G             L
Sbjct: 150 RNMVPDIESIAQDSLRSWEGTM---INTYQEMKTYTFNVALLSIFGKD---------EVL 209

Query: 215 YKEMSFRVV------MSTFLYDFPGFTFRQALKARKKIENIIQGVVEEKRRRFENDKTSE 274
           Y+E   R         ++   + PG  F +++KARK++  I+  ++ E+R+   +     
Sbjct: 210 YREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLL 269

Query: 275 VQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFWALLHISQNPHVFQK 334
                DK          +   D  I D ++GV FA   T A    W L ++++NP+V + 
Sbjct: 270 GSFMGDK----------EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEA 329

Query: 335 AKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRNTITPTNFREAKTDANINGY 394
             EEQ + IR+     + LT  + K+M   S+VI E LR  +I    FREA  D    GY
Sbjct: 330 VTEEQMA-IRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGY 389

Query: 395 FIPKGWTI-----QIWNIAN--------------YTPKPGTFIPFGLGSRFCPGSDFSRL 454
            IPKGW +      I + A+                PKP TF+PFG G+  CPG++ ++L
Sbjct: 390 LIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKL 449

Query: 455 EISILLHHFILNYKMEGVNLESYITQMPSPRPENCL 464
           E+SI++HH    Y    V     I   P   P+N L
Sbjct: 450 EMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGL 455

[Close]

BLAST of IVF0000182 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 179.1 bits (453), Expect = 8.3e-45
Identity = 126/434 (29.03%), Postives = 197/434 (45.39%), Query Frame = 0


Query: 35  GRAYKSLPPGDLGWPVIGSSFSFYKTFVVQEDPISFIQSLHSRYGKGGIYKTHLYGKPTV 94
           G +   LPPG +GWP +G +F  Y      +DP  F QS   RY  G ++KTH+ G P V
Sbjct: 30  GSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRY--GSVFKTHVLGCPCV 89

Query: 95  IATDPEICRRIYLDEAK-FKQHYPKSVKILEGSSG-EFSKMDHKIAYKLMAAPMNGSEVL 154
           + + PE  + + + ++  FK  +P S + + G     F + D+    + +       E +
Sbjct: 90  MISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESI 149

Query: 155 SNYVDFIEEVIAKGLEEWSSMMRDPIKLLEEIGNLFFKVIIKVFLGSQLDAKTMVELHTL 214
            N V  IE +    L  W   M   I   +E+    F V +    G             L
Sbjct: 150 RNMVPDIESIAQDSLRSWEGTM---INTYQEMKTYTFNVALLSIFGKD---------EVL 209

Query: 215 YKEMSFRVV------MSTFLYDFPGFTFRQALKARKKIENIIQGVVEEKRRRFENDKTSE 274
           Y+E   R         ++   + PG  F +++KARK++  I+  ++ E+R+   +     
Sbjct: 210 YREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLL 269

Query: 275 VQCQVDKMVVAIDENGAKLYNDNLIRDLLLGVFFAGYSTPAIAAFWALLHISQNPHVFQK 334
                DK          +   D  I D ++GV FA   T A    W L ++++NP+V + 
Sbjct: 270 GSFMGDK----------EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEA 329

Query: 335 AKEEQESIIRQRPSNQKGLTLNEIKQMKYLSKVINEVLRRNTITPTNFREAKTDANINGY 394
             EEQ + IR+     + LT  + K+M   S+VI E LR  +I    FREA  D    GY
Sbjct: 330 VTEEQMA-IRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGY 389

Query: 395 FIPKGWTI-----QIWNIAN--------------YTPKPGTFIPFGLGSRFCPGSDFSRL 442
            IPKGW +      I + A+                PKP TF+PFG G+  CPG++ ++L
Sbjct: 390 LIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKL 433

The following BLAST results are available for this feature:

BLAST of IVF0000182 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.2e-101	42.51	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	1.8e-92	41.91	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q5VRM7	8.2e-90	35.98	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9AXH9	5.0e-87	35.69	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]
O23051	3.6e-85	38.45	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]

BLAST of IVF0000182 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A097IYP6	2.3e-236	84.49	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1	[more]
A0A1S3CRX8	5.5e-230	88.24	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1	[more]
A0A5D3E6A2	1.7e-183	100.00	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A5A7T6J1	6.6e-183	99.70	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A5A7T517	1.2e-173	97.48	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]

BLAST of IVF0000182 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148515.2	1.70e-299	84.49	beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sa...	[more]
XP_008466645.1	4.59e-292	88.24	PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo]	[more]
TYK31140.1	3.60e-233	100.00	beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa]	[more]
KAA0038543.1	2.08e-232	99.70	beta-amyrin 11-oxidase-like [Cucumis melo var. makuwa]	[more]
XP_011652660.1	1.89e-231	65.31	beta-amyrin 11-oxidase [Cucumis sativus]	[more]

BLAST of IVF0000182 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	1.3e-93	41.91	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	1.3e-93	41.91	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.5e-86	38.45	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT4G19230.1	3.4e-46	28.95	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.2	8.3e-45	29.03	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 419..442 score: 21.45 coord: 77..96 score: 20.64 coord: 290..307 score: 26.19 coord: 310..336 score: 25.71 coord: 409..419 score: 54.21
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 410..419 score: 56.28 coord: 301..318 score: 27.54 coord: 358..369 score: 36.04 coord: 419..430 score: 32.07
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 42..397 e-value: 9.0E-47 score: 159.9 coord: 405..444 e-value: 5.7E-8 score: 31.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..467 e-value: 1.3E-75 score: 256.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..446
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 15..455
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 15..455
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 412..421

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0000182.1	IVF0000182.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051777	ent-kaurenoate oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen