HG10023539 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023539 vs. NCBI nr
Match: XP_038896284.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1572.8 bits (4071), Expect = 0.0e+00
Identity = 840/935 (89.84%), Postives = 861/935 (92.09%), Query Frame = 0

Query: 1   MACERCLTLSSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKNNLNLLE 60
           MACER LTLSSPFP SRLGILKPRTWRRPY SISSQIS PS SQSG+ +DSKKNNLNLLE
Sbjct: 1   MACERFLTLSSPFPYSRLGILKPRTWRRPYPSISSQISTPSNSQSGKVDDSKKNNLNLLE 60

Query: 61  LSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQGLPSVS 120
           LSVTLTI+STSLP+SSALAATASKEVKERRRGPKKSS KKVEALSPQELLSWSQGLPS+S
Sbjct: 61  LSVTLTIISTSLPMSSALAATASKEVKERRRGPKKSSAKKVEALSPQELLSWSQGLPSIS 120

Query: 121 NRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVESNRRFWE 180
           NRIPYTEVLD KREGK+KHVIKVPNGFLR+RSEPVMVVLEDSRVLRTVLPSVESNRRFWE
Sbjct: 121 NRIPYTEVLDFKREGKLKHVIKVPNGFLRVRSEPVMVVLEDSRVLRTVLPSVESNRRFWE 180

Query: 181 LWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAELRRVRE 240
           LWDELGIDSVCVNAYTPPIKPPDVPTPYL FL+RVPEFMY FVKPKKESKRAAELRRVRE
Sbjct: 181 LWDELGIDSVCVNAYTPPIKPPDVPTPYLAFLSRVPEFMYFFVKPKKESKRAAELRRVRE 240

Query: 241 ELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKNYQHMAI 300
           ELKMEK+ ELAKMRQEREMIEKAMK+QKKEEERRI++ETRRKKQEESLREARKNYQ MA+
Sbjct: 241 ELKMEKSAELAKMRQEREMIEKAMKVQKKEEERRIRKETRRKKQEESLREARKNYQRMAL 300

Query: 301 VWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER 360
           VWANLARDSNV+TALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER
Sbjct: 301 VWANLARDSNVSTALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER 360

Query: 361 ELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA 420
           E+EGIEGEDDD+EQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA
Sbjct: 361 EVEGIEGEDDDVEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA 420

Query: 421 GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS 480
           GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS
Sbjct: 421 GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS 480

Query: 481 ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL 540
           ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL
Sbjct: 481 ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL 540

Query: 541 NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA 600
           NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA
Sbjct: 541 NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA 600

Query: 601 RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG 660
           RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG
Sbjct: 601 RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG 660

Query: 661 MLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAI-- 720
           MLD KERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAI  
Sbjct: 661 MLDRKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAIKY 720

Query: 721 -----NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSSSDD 780
                 RQSLLDHITVQLAPRAADELWHGE Q                            
Sbjct: 721 NEGMLTRQSLLDHITVQLAPRAADELWHGEAQ---------------------------- 780

Query: 781 FSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADR 840
                                LSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADR
Sbjct: 781 ---------------------LSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADR 840

Query: 841 INDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP 900
           INDID+EALRI+N+CY+RAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP
Sbjct: 841 INDIDLEALRIVNICYERAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP 885

Query: 901 MPLSIIDLRIAKRTKFEEEMMKKNQKKIPVGSNSS 929
           MP SIIDLRIAKRTKFEEEMM KNQKKIP+ SNSS
Sbjct: 901 MPPSIIDLRIAKRTKFEEEMM-KNQKKIPISSNSS 885

BLAST of HG10023539 vs. NCBI nr
Match: XP_022146651.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic [Momordica charantia])

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 808/941 (85.87%), Postives = 836/941 (88.84%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER   L      SSPFPNSRLGILK R WRRPY SISSQIS PS  +  E  DSKKN
Sbjct: 1   MACERFHNLSSSIYSSSPFPNSRLGILKQRRWRRPYPSISSQISTPSNFRGDEDEDSKKN 60

Query: 61  NL-NLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWS 120
            L N L+LSVTLTI+S S PISSA AATA+KEVKERRRG KKSS KK+EALSPQEL+SWS
Sbjct: 61  RLKNFLKLSVTLTIVSASFPISSAHAATAAKEVKERRRGTKKSSAKKIEALSPQELJSWS 120

Query: 121 QGLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVE 180
           QGLP VSNRIPYTE+LDLKREGKVKHVI+VP GF R RSEPVMVVLEDSRVLRTVLPS E
Sbjct: 121 QGLPPVSNRIPYTEILDLKREGKVKHVIRVPYGFFRQRSEPVMVVLEDSRVLRTVLPSGE 180

Query: 181 SNRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAA 240
           +NRRFWELWDELGID+VCVNAYTPPIKPPD+P PYLGFL RVP FMYSFVKPKKESKRAA
Sbjct: 181 NNRRFWELWDELGIDTVCVNAYTPPIKPPDLPAPYLGFLLRVPAFMYSFVKPKKESKRAA 240

Query: 241 ELRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARK 300
           E+RRVREELKMEKTVELAKMRQEREMIEKA+KMQKK+EERRIKR+TRRKK+EESLREARK
Sbjct: 241 EIRRVREELKMEKTVELAKMRQEREMIEKALKMQKKQEERRIKRQTRRKKEEESLREARK 300

Query: 301 NYQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK 360
           NYQHMAIVW NL+RDSNVAT LGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK
Sbjct: 301 NYQHMAIVWTNLSRDSNVATTLGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK 360

Query: 361 KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN 420
           KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN
Sbjct: 361 KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN 420

Query: 421 VKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE 480
           VKFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE
Sbjct: 421 VKFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE 480

Query: 481 AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG 540
           AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG
Sbjct: 481 AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG 540

Query: 541 QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRM 600
           QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+
Sbjct: 541 QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRI 600

Query: 601 EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQA 660
           EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAA+NMIRD RTEITTDDLLQA
Sbjct: 601 EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAAINMIRDSRTEITTDDLLQA 660

Query: 661 AQIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM 720
           AQIEERGMLD KERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM
Sbjct: 661 AQIEERGMLDRKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM 720

Query: 721 KMNAI-------NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNK 780
           KMNAI        RQSLLDHITVQLAPRAADELW+G  Q                     
Sbjct: 721 KMNAIKYNEGMLTRQSLLDHITVQLAPRAADELWYGAHQ--------------------- 780

Query: 781 MGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVS 840
                                       LSTIWAETADNARSAARTFVLGGLSEKH+GVS
Sbjct: 781 ----------------------------LSTIWAETADNARSAARTFVLGGLSEKHYGVS 840

Query: 841 NFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVE 900
           NFWVADRINDID+EALRILNVCY+RAKEILQQNR LMDAVVDDLIQKKSLTK+EF  LVE
Sbjct: 841 NFWVADRINDIDLEALRILNVCYERAKEILQQNRTLMDAVVDDLIQKKSLTKREFFHLVE 892

Query: 901 LHGSVKPMPLSIIDLRIAKRTKFEEEMMKKNQKKIPVGSNS 928
           LHGS+KPMP SIIDLRIAKRTKF+EEMMK NQKKI +GS+S
Sbjct: 901 LHGSIKPMPPSIIDLRIAKRTKFQEEMMKINQKKIAIGSSS 892

BLAST of HG10023539 vs. NCBI nr
Match: XP_038896286.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 799/935 (85.45%), Postives = 820/935 (87.70%), Query Frame = 0

Query: 1   MACERCLTLSSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKNNLNLLE 60
           MACER LTLSSPFP SRLGILKPRTWRRPY SISSQIS PS SQSG+ +DSKKNNLNLLE
Sbjct: 1   MACERFLTLSSPFPYSRLGILKPRTWRRPYPSISSQISTPSNSQSGKVDDSKKNNLNLLE 60

Query: 61  LSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQGLPSVS 120
           LSVTLTI+STSLP+SSALAATASKEVKERRRGPKKSS KKVEALSPQELLSWSQGLPS+S
Sbjct: 61  LSVTLTIISTSLPMSSALAATASKEVKERRRGPKKSSAKKVEALSPQELLSWSQGLPSIS 120

Query: 121 NRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVESNRRFWE 180
           NRIPYTEVLD KREGK+KHVIKVPNGFLR+RSEPVMVVLEDSRVLRTVLPSVESNRRFWE
Sbjct: 121 NRIPYTEVLDFKREGKLKHVIKVPNGFLRVRSEPVMVVLEDSRVLRTVLPSVESNRRFWE 180

Query: 181 LWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAELRRVRE 240
           LWDELGIDSVCVNAYTPPIKPPDVPTPYL FL+RVPEFMY FVKPKKESKRAAELRRVRE
Sbjct: 181 LWDELGIDSVCVNAYTPPIKPPDVPTPYLAFLSRVPEFMYFFVKPKKESKRAAELRRVRE 240

Query: 241 ELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKNYQHMAI 300
           ELKMEK+ ELAKMRQEREMIEKAMK+QKKEEERRI++ETRRKKQEESLREARKNYQ MA+
Sbjct: 241 ELKMEKSAELAKMRQEREMIEKAMKVQKKEEERRIRKETRRKKQEESLREARKNYQRMAL 300

Query: 301 VWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER 360
           VWANLARDSNV+TALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER
Sbjct: 301 VWANLARDSNVSTALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELER 360

Query: 361 ELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA 420
           E+EGIEGEDDD+EQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA
Sbjct: 361 EVEGIEGEDDDVEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVA 420

Query: 421 GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS 480
           GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS
Sbjct: 421 GLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFS 480

Query: 481 ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL 540
           ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL
Sbjct: 481 ISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATL 540

Query: 541 NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA 600
           NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA
Sbjct: 541 NQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHA 600

Query: 601 RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG 660
           RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG
Sbjct: 601 RKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERG 660

Query: 661 MLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAI-- 720
           MLD KERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAI  
Sbjct: 661 MLDRKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNAIKY 720

Query: 721 -----NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSSSDD 780
                 RQSLLDHITVQLAPRAADELWHGE Q                            
Sbjct: 721 NEGMLTRQSLLDHITVQLAPRAADELWHGEAQ---------------------------- 780

Query: 781 FSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADR 840
                                                                       
Sbjct: 781 ------------------------------------------------------------ 840

Query: 841 INDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP 900
             DID+EALRI+N+CY+RAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP
Sbjct: 841 --DIDLEALRIVNICYERAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVKP 844

Query: 901 MPLSIIDLRIAKRTKFEEEMMKKNQKKIPVGSNSS 929
           MP SIIDLRIAKRTKFEEEMM KNQKKIP+ SNSS
Sbjct: 901 MPPSIIDLRIAKRTKFEEEMM-KNQKKIPISSNSS 844

BLAST of HG10023539 vs. NCBI nr
Match: XP_004141654.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic [Cucumis sativus])

HSP 1 Score: 1452.6 bits (3759), Expect = 0.0e+00
Identity = 782/936 (83.55%), Postives = 828/936 (88.46%), Query Frame = 0

Query: 1   MACERCLTLSSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDS-KKNNLNLL 60
           MACER LTLSSPF ++RLG LKPRTWRRP+ SISSQIS PS S + E NDS KKN LN L
Sbjct: 1   MACERFLTLSSPFSSARLGTLKPRTWRRPHPSISSQISTPSDSPTDEHNDSKKKNKLNFL 60

Query: 61  ELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQGLPSV 120
           ++SVTL+ILSTSLP+SSALAA ASKEVKERRRGPK+SS KK E+LSPQELLSWSQGLP++
Sbjct: 61  QISVTLSILSTSLPMSSALAAAASKEVKERRRGPKRSSAKKAESLSPQELLSWSQGLPAI 120

Query: 121 SNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVESNRRFW 180
           SNRIPYTE+LDLKREGKVKHVIK PNGFLR+RSE VMV+LEDSRVLRTVLPSVESNRRFW
Sbjct: 121 SNRIPYTELLDLKREGKVKHVIKNPNGFLRLRSELVMVILEDSRVLRTVLPSVESNRRFW 180

Query: 181 ELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAELRRVR 240
            LW+ELGIDSVCVNAYTPPIKPP++PTPYLGFLARVP FM+ F +PKKESKR A+LRR+R
Sbjct: 181 VLWNELGIDSVCVNAYTPPIKPPELPTPYLGFLARVPFFMFYFGQPKKESKRVAQLRRLR 240

Query: 241 EELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKNYQHMA 300
           +E+KME T EL KMRQE E IEKAMKMQKK+EERRIKRETRRKKQ ESLREARK  ++M 
Sbjct: 241 DEVKMETTTELTKMRQENEKIEKAMKMQKKQEERRIKRETRRKKQVESLREARKISENMG 300

Query: 301 IVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRELE 360
           ++W  LA   NVA ALGLVFFVIFYRTVV SYRRQKKDYEDRLKIE+AEAEERKKM ELE
Sbjct: 301 MIWEELANSPNVAFALGLVFFVIFYRTVVLSYRRQKKDYEDRLKIEEAEAEERKKMLELE 360

Query: 361 RELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDV 420
            +LE  EGEDDDIEQGKGEQNPYLKMA QFMKSGARVRRAHGKRLPQYLE+GVNVKFEDV
Sbjct: 361 TDLEVTEGEDDDIEQGKGEQNPYLKMATQFMKSGARVRRAHGKRLPQYLEKGVNVKFEDV 420

Query: 421 AGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFF 480
           AGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILL GPPGVGKTLLAKAVAGEAGVNFF
Sbjct: 421 AGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLSGPPGVGKTLLAKAVAGEAGVNFF 480

Query: 481 SISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDAT 540
           SISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDAT
Sbjct: 481 SISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDAT 540

Query: 541 LNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVH 600
           LNQLLVCLDGFEGRGEVITIASTNR DILDPALVRPGRFDRKIYIPKPGLIGRMEILKVH
Sbjct: 541 LNQLLVCLDGFEGRGEVITIASTNRSDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVH 600

Query: 601 ARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEER 660
           ARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIR+GRTEITTDDLLQAAQIEER
Sbjct: 601 ARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIREGRTEITTDDLLQAAQIEER 660

Query: 661 GMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNA-- 720
           G+LD KERSP+TWKQVAINEAAMAVVAVNFPDL+NIEFVTIAPR+GRELGYVRMKMNA  
Sbjct: 661 GLLDRKERSPDTWKQVAINEAAMAVVAVNFPDLENIEFVTIAPRSGRELGYVRMKMNAMK 720

Query: 721 -----INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSSSD 780
                + RQSLLDHITVQLAPRAADELWHGEDQ                           
Sbjct: 721 YNEGMLTRQSLLDHITVQLAPRAADELWHGEDQ--------------------------- 780

Query: 781 DFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVAD 840
                                 LSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVAD
Sbjct: 781 ----------------------LSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVAD 840

Query: 841 RINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGSVK 900
           RINDID+EALRIL+VCY+RAKEILQQNRKLMDAVVD LIQKKSL+KQEFL+LV+LHGS+K
Sbjct: 841 RINDIDLEALRILSVCYERAKEILQQNRKLMDAVVDGLIQKKSLSKQEFLRLVKLHGSIK 886

Query: 901 PMPLSIIDLRIAKRTKFEEEMMKKNQKKIPVGSNSS 929
           PM  SIIDLRIAKR KF+EE MKKNQKKIPVGSNSS
Sbjct: 901 PMSPSIIDLRIAKRAKFDEE-MKKNQKKIPVGSNSS 886

BLAST of HG10023539 vs. NCBI nr
Match: XP_022992096.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1450.3 bits (3753), Expect = 0.0e+00
Identity = 779/942 (82.70%), Postives = 823/942 (87.37%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER  T       SSPFPNSRL ILKPRTWRRPY S+SSQIS PS SQS E NDSKKN
Sbjct: 1   MACERFHTFSSSIYSSSPFPNSRLRILKPRTWRRPYPSVSSQISSPSNSQSDEDNDSKKN 60

Query: 61  NLNLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQ 120
            LNLL+LSVTLT++STSL  S+ALAATASKEVK RRRG KKSS KK +ALSPQELLSWSQ
Sbjct: 61  KLNLLKLSVTLTVVSTSLHTSNALAATASKEVKGRRRGAKKSSTKKGDALSPQELLSWSQ 120

Query: 121 GLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVES 180
           GLPSVSNRIPYTE+LDLKREGKVKHVIKVPNGFLR+R EPVMVVLEDSRVLRTVLPSVES
Sbjct: 121 GLPSVSNRIPYTELLDLKREGKVKHVIKVPNGFLRLRPEPVMVVLEDSRVLRTVLPSVES 180

Query: 181 NRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAE 240
           N RFW LWDELGIDSVCVNAYTPP+K PDVPTPYLGFL RVP F+YSFVKPKKESKRAAE
Sbjct: 181 NGRFWVLWDELGIDSVCVNAYTPPVKLPDVPTPYLGFLTRVPAFLYSFVKPKKESKRAAE 240

Query: 241 LRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKN 300
           +RR+REE+KMEKT  L KMRQEREMIEK +KMQKKEE+ RI+RE  RKK+E+SL EAR N
Sbjct: 241 VRRIREEIKMEKTAALEKMRQEREMIEKTVKMQKKEEDMRIRREANRKKREQSLNEARIN 300

Query: 301 YQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKK 360
           YQ MA  WA LARD NV +ALG +FFVIFY+TVV SYRRQKKDYEDRLKIEKAEAEERKK
Sbjct: 301 YQRMANFWAELARDPNVVSALGFLFFVIFYQTVVLSYRRQKKDYEDRLKIEKAEAEERKK 360

Query: 361 MRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNV 420
           MR+LERE+EG+EGEDDD+E GKGEQNPYLKMA QFMKSGARVRR+HGKR  QYLERG+NV
Sbjct: 361 MRDLEREMEGLEGEDDDVEPGKGEQNPYLKMAAQFMKSGARVRRSHGKRTLQYLERGMNV 420

Query: 421 KFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480
           KFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA
Sbjct: 421 KFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480

Query: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQ 540
           GVNFFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQ
Sbjct: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQ 540

Query: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRME 600
           ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+E
Sbjct: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRLE 600

Query: 601 ILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660
           ILKVHARKKPMA D+DYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA
Sbjct: 601 ILKVHARKKPMANDLDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660

Query: 661 QIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMK 720
           QIEERGMLD KERS ETWKQVA+NEAAMAVVA+NFPDLKNIEFVTIAPRAGRELGYVRMK
Sbjct: 661 QIEERGMLDKKERSLETWKQVALNEAAMAVVAMNFPDLKNIEFVTIAPRAGRELGYVRMK 720

Query: 721 MNA-------INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKM 780
           M+A       + RQSLLDHITVQLAPRAADELW+GEDQ                      
Sbjct: 721 MDAMRFNEGMLTRQSLLDHITVQLAPRAADELWYGEDQ---------------------- 780

Query: 781 GSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSN 840
                                      LSTIW ETADNARSAARTFVLGGLSEKH+GVSN
Sbjct: 781 ---------------------------LSTIWVETADNARSAARTFVLGGLSEKHYGVSN 840

Query: 841 FWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVEL 900
           FWVADRINDID+EALRIL+VCY+RAKEILQQNRKLMD V+DDLIQKKSLTK EFL LVEL
Sbjct: 841 FWVADRINDIDLEALRILHVCYERAKEILQQNRKLMDVVIDDLIQKKSLTKNEFLHLVEL 893

Query: 901 HGSVKPMPLSIIDLRIAKRTKF-EEEMMKKNQKKIPVGSNSS 929
           HGS+KPMP SIIDLRIAKRTK  EEE MKKNQKKI VGS+++
Sbjct: 901 HGSIKPMPPSIIDLRIAKRTKLQEEEKMKKNQKKIAVGSSNT 893

BLAST of HG10023539 vs. ExPASy Swiss-Prot
Match: A8MPR5 (Probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSHI2 PE=1 SV=1)

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 654/925 (70.70%), Postives = 752/925 (81.30%), Query Frame = 0

Query: 1   MACERCLTLSSPFPNSRLGILKPRTWRRPYASISSQISIPS--YSQSGEGND-SKKNNLN 60
           MAC     L S  P+  L     +   R Y SIS Q +  +    + G+ ND +K N +N
Sbjct: 1   MACR--FPLHSSSPSQFLSPENRQRLPRNYPSISCQNNSATNVVHEDGDDNDKAKTNQVN 60

Query: 61  LLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQGLP 120
           LL + +TLTI+S SL    A  + A+ +V ER+R  KK      EAL+ ++L +WS+ LP
Sbjct: 61  LLAIPITLTIISASL----AKPSFAAAKVTERKRTQKKPQ----EALTLEQLKAWSKDLP 120

Query: 121 SVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVESNRR 180
            VSNRIPYT++L LK EGK+KHVIK PN  LR ++EPV+VVLEDSRVLRTVLPS+E N+R
Sbjct: 121 VVSNRIPYTDILSLKAEGKLKHVIKPPNLSLRQKAEPVLVVLEDSRVLRTVLPSLEGNKR 180

Query: 181 FWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAELRR 240
           FWE WDELGID  CVNAYTPP+K P VP+PYLGFL +VP +M ++VKPKKESKRAAEL+R
Sbjct: 181 FWEQWDELGIDVQCVNAYTPPVKRPPVPSPYLGFLWKVPAYMLTWVKPKKESKRAAELKR 240

Query: 241 VREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKNYQH 300
           +RE+ K ++  E+  M++ER M+EK MK QKK++ER+ ++  R+KK EESLREARKNY+ 
Sbjct: 241 MREDFKRQRKEEIETMKEERVMMEKTMKAQKKQQERKKRKAVRKKKYEESLREARKNYRD 300

Query: 301 MAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRE 360
           MA +WA LA+D NVATALGLVFF IFYR VV +YR+QKKDYEDRLKIEKAEA+ERKKMRE
Sbjct: 301 MADMWARLAQDPNVATALGLVFFYIFYRVVVLNYRKQKKDYEDRLKIEKAEADERKKMRE 360

Query: 361 LERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFE 420
           LERE+EGIE ED+++E+G GE+NPYL+MAMQFMKSGARVRRA  KRLP+YLERGV+VKF 
Sbjct: 361 LEREMEGIEEEDEEVEEGTGEKNPYLQMAMQFMKSGARVRRASNKRLPEYLERGVDVKFT 420

Query: 421 DVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN 480
           DVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN
Sbjct: 421 DVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN 480

Query: 481 FFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERD 540
           FFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQERD
Sbjct: 481 FFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQERD 540

Query: 541 ATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILK 600
           ATLNQLLV LDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKI+IPKPGLIGRMEIL+
Sbjct: 541 ATLNQLLVSLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIFIPKPGLIGRMEILQ 600

Query: 601 VHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIE 660
           VHARKKPMAED+DYMAVASMTDGMVGAELANIVE+AA+NM+RDGRTE+TTDDLLQAAQIE
Sbjct: 601 VHARKKPMAEDLDYMAVASMTDGMVGAELANIVEIAAINMMRDGRTELTTDDLLQAAQIE 660

Query: 661 ERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNA 720
           ERGMLD K+RS ETW+QVAINEAAMAVVAVNFPD+KNIEF+TI PRAGRELGYVR+KM+ 
Sbjct: 661 ERGMLDRKDRSLETWRQVAINEAAMAVVAVNFPDMKNIEFLTINPRAGRELGYVRVKMDH 720

Query: 721 I-------NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSS 780
           I       +RQS+LDHITVQLAPRAADELW+GEDQ                         
Sbjct: 721 IKFKEGMLSRQSILDHITVQLAPRAADELWYGEDQ------------------------- 780

Query: 781 SDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWV 840
                                   LSTIWAET+DNARSAAR+ VLGGLS+KHHG++NFWV
Sbjct: 781 ------------------------LSTIWAETSDNARSAARSLVLGGLSDKHHGLNNFWV 840

Query: 841 ADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGS 900
           ADRINDID+EALRILN+CY+RAKEIL +NR LMD VV+ L+QKKSLTKQEF  LVEL+GS
Sbjct: 841 ADRINDIDVEALRILNMCYERAKEILGRNRTLMDEVVEKLVQKKSLTKQEFFTLVELYGS 866

Query: 901 VKPMPLSIIDLRIAKRTKFEEEMMK 916
            KPMP SI++LR  KR + EE ++K
Sbjct: 901 SKPMPPSILELRKIKRLELEEMVLK 866

BLAST of HG10023539 vs. ExPASy Swiss-Prot
Match: O67077 (ATP-dependent zinc metalloprotease FtsH OS=Aquifex aeolicus (strain VF5) OX=224324 GN=ftsH PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.2e-85
Identity = 201/509 (39.49%), Postives = 297/509 (58.35%), Query Frame = 0

Query: 390 MKSGARVRRAHG---KRLPQYLERGVNVKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRG 449
           M  G  V RA      R   Y+E    V F+DVAG+ +++ E++EI+++      +++ G
Sbjct: 125 MSGGGNVNRAFNFGKSRAKVYIEEKPKVTFKDVAGIEEVKEEVKEIIEYLKDPVKFQKLG 184

Query: 450 VKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFSISASQFVEIYVGVGASRVRALYQEAK 509
            + P G+LL G PGVGKTLLAKA+AGEA V F S+S S FVE++VGVGA+RVR L++ AK
Sbjct: 185 GRPPKGVLLYGEPGVGKTLLAKAIAGEAHVPFISVSGSDFVEMFVGVGAARVRDLFETAK 244

Query: 510 ENAPSVVFIDELDAVGRERGLIK-GSGGQERDATLNQLLVCLDGFEGRGEVITIASTNRP 569
           ++AP ++FIDE+DAVGR RG I  G G  ER+ TLNQLLV +DGF+    +I IA+TNRP
Sbjct: 245 KHAPCIIFIDEIDAVGRARGAIPVGGGHDEREQTLNQLLVEMDGFDTSDGIIVIAATNRP 304

Query: 570 DILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHARKKPMAEDVDYMAVASMTDGMVGAE 629
           DILDPAL+RPGRFDR+I+IPKP + GR EILKVHAR K +A+DVD   VA  T G  GA+
Sbjct: 305 DILDPALLRPGRFDRQIFIPKPDVRGRYEILKVHARNKKLAKDVDLEFVARATPGFTGAD 364

Query: 630 LANIVEVAALNMIRDGRTEITTDDLLQAAQIEERGM-LDGKERSPETWKQVAINEAAMAV 689
           L N++  AAL   R G+ EIT +++ +A      G+   G   SP+  +++AI+EA  A+
Sbjct: 365 LENLLNEAALLAARKGKEEITMEEIEEALDRITMGLERKGMTISPKEKEKIAIHEAGHAL 424

Query: 690 VAVNFPDLKNIEFVTIAPRAGRELGYVRM----KMNAINRQSLLDHITVQLAPRAADELW 749
           + +   D   +  ++I PR G  LG  +       +  +++ L + I V L  RAA+E++
Sbjct: 425 MGLVSDDDDKVHKISIIPR-GMALGVTQQLPIEDKHIYDKKDLYNKILVLLGGRAAEEVF 484

Query: 750 HGEDQMANNRSSTPVGIPDWPKIYNKMGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWA 809
            G+D +     +      D       M   S     DK   I                  
Sbjct: 485 FGKDGITTGAENDLQRATDLAYRMVSMWGMS-----DKVGPI------------------ 544

Query: 810 ETADNARSAARTFVLGGLSEKHHGVSNFWVADRINDIDMEALRILNVCYDRAKEILQQNR 869
                 R  A  F LGG++      +     D + +ID E  RI+   Y++AK I+++ +
Sbjct: 545 ----AIRRVANPF-LGGMT-----TAVDTSPDLLREIDEEVKRIITEQYEKAKAIVEEYK 599

Query: 870 KLMDAVVDDLIQKKSLTKQEFLQLVELHG 890
           + + AVV  L++K+++T +EF+++ +L+G
Sbjct: 605 EPLKAVVKKLLEKETITCEEFVEVFKLYG 599

BLAST of HG10023539 vs. ExPASy Swiss-Prot
Match: Q2JNP0 (ATP-dependent zinc metalloprotease FtsH OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=ftsH PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.5e-82
Identity = 203/535 (37.94%), Postives = 289/535 (54.02%), Query Frame = 0

Query: 379 QNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVAGLGKIRLELEEIVKFFTQ 438
           QN     A+ F KS ARV+          +E    + F DVAG+ + +LEL E+V F   
Sbjct: 141 QNGPGSQALNFGKSRARVQ----------MEPKTQITFNDVAGIDQAKLELAEVVDFLKN 200

Query: 439 GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFSISASQFVEIYVGVGASRV 498
            E +   G KIP G+LL GPPG GKTLLA+AVAGEAGV FFSIS S+FVE++VGVGASRV
Sbjct: 201 SERFTALGAKIPRGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSEFVEMFVGVGASRV 260

Query: 499 RALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATLNQLLVCLDGFEGRGEVIT 558
           R L+++AK+NAP +VFIDE+DAVGR+RG   G G  ER+ TLNQLL  +DGFEG   +I 
Sbjct: 261 RDLFEQAKQNAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLTEMDGFEGNSGIIV 320

Query: 559 IASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHARKKPMAEDVDYMAVASMT 618
           IA+TNRPD+LD AL+RPGRFDR++ + +P   GR+EILKVHAR K ++ DVD   +A  T
Sbjct: 321 IAATNRPDVLDAALLRPGRFDRQVTVDRPDFQGRLEILKVHARGKTLSADVDLEKLARRT 380

Query: 619 DGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERGMLDGKERSPETWKQ---- 678
            G  GA+LAN++  AA+   R   TEI+ D++  A       +L G E+      +    
Sbjct: 381 PGFTGADLANLLNEAAILAARRNLTEISMDEINDAVD----RVLAGPEKKDRLMSERRKE 440

Query: 679 -VAINEAAMAVVAVNFPDLKNIEFVTIAPR--AGRELGYVRM--KMNAINRQSLLDHITV 738
            VA +EA  A+V    P+   I+ VTI PR  AG    ++     M    R  L + +TV
Sbjct: 441 LVAYHEAGHALVGSLLPNYDPIQKVTIIPRGQAGGLTWFMPSDDDMGLTTRAHLKNMMTV 500

Query: 739 QLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSSSDDFSGDKTDDIDQEDIDG 798
            L  R A+E+ +GE ++    +S      D  ++     +    F              G
Sbjct: 501 ALGGRVAEEVVYGESEITTGAAS------DLQQVARIARNMVTRF--------------G 560

Query: 799 EEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADRINDIDMEALRILNVCY 858
             D + +        N             SE+   +           ID E  R++N  Y
Sbjct: 561 MSDRLGNVALGRQYANIFLGREIAAERDFSEETAAL-----------IDEEVRRLVNEAY 620

Query: 859 DRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVE------LHGSVKPMPLSI 899
            RA  ++++NR L+D +   L++ +++  +E   +++      L    +P PL++
Sbjct: 621 QRATYLIRENRALLDRIARRLVEAETIDGEELQAIIDNSEVVMLPPEEEPEPLTL 630

BLAST of HG10023539 vs. ExPASy Swiss-Prot
Match: Q67JH0 (ATP-dependent zinc metalloprotease FtsH 3 OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=ftsH3 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.9e-82
Identity = 214/535 (40.00%), Postives = 301/535 (56.26%), Query Frame = 0

Query: 372 IEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVAGLGKIRLELEE 431
           ++Q +G  N      MQF KS AR+     KR          V F+DVAG+ +++ EL E
Sbjct: 126 MQQTQGSGN----RVMQFGKSRARLVTDDRKR----------VTFDDVAGIDEVKEELAE 185

Query: 432 IVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFSISASQFVEIYV 491
           IV F    + Y   G +IP G+LL GPPG GKTLLAKAVAGEAGV FFSIS S FVE++V
Sbjct: 186 IVDFLKHPKRYLELGARIPKGVLLYGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFV 245

Query: 492 GVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATLNQLLVCLDGFE 551
           GVGASRVR L+++AK+N+P +VFIDE+DAVGR+RG   G G  ER+ TLNQLLV +DGF 
Sbjct: 246 GVGASRVRDLFEQAKKNSPCIVFIDEIDAVGRQRGAGYGGGHDEREQTLNQLLVEMDGFS 305

Query: 552 GRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHARKKPMAEDVDY 611
               +I IA+TNRPD+LDPAL+RPGRFDR+I I +P L GR+ I +VHA+ KP+  DVD 
Sbjct: 306 ANEGIIIIAATNRPDVLDPALLRPGRFDRQIVIDRPDLKGRLAIFQVHAKGKPLEPDVDL 365

Query: 612 MAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERGMLDGKER--SP 671
             +A  T G  GA++AN++  AAL   R  + +I+  D+  A      G  + K R  S 
Sbjct: 366 EVLAKRTPGFTGADIANLMNEAALLAARRRKKKISMQDVEDAIDRVLAGGPEKKSRVISE 425

Query: 672 ETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGY-----VRMKMNAINRQSLL 731
           +  +  A +EA  AVV    P +  +  +TI PR GR +GY     V  + N I++  +L
Sbjct: 426 KEKRVTAYHEAGHAVVGHMLPHMDPLHKITIIPR-GRAMGYTLFLPVEDRYN-ISKSEIL 485

Query: 732 DHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWP-KIYNKMGSSSDDFSGDKTDDID 791
           D +T+ L  RAA+E+  GE  + +           W  ++  + G S  +  G  T  + 
Sbjct: 486 DRMTMALGGRAAEEITFGE--ITSGAQDDIERTTQWARRMVTEWGMS--EKLGPLTYGMK 545

Query: 792 QEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWVADRINDIDMEALR 851
           Q+++                  AR   R   L   SE+  G+           ID E  +
Sbjct: 546 QDEV----------------FLARDMTR---LRNYSEEVAGL-----------IDEEVRK 605

Query: 852 ILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVE--LHGSVKPMPL 897
            +++ Y RA +IL ++R  ++ V + L++K++L  +E   L+E  L    KP PL
Sbjct: 606 FVHMAYQRAIDILTEHRDALEKVSEVLLEKETLEGKELQDLLEQLLPPRPKPEPL 610

BLAST of HG10023539 vs. ExPASy Swiss-Prot
Match: P72991 (ATP-dependent zinc metalloprotease FtsH 3 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=ftsH3 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.9e-82
Identity = 204/539 (37.85%), Postives = 288/539 (53.43%), Query Frame = 0

Query: 373 EQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFEDVAGLGKIRLELEEI 432
           + G G Q      AM F KS ARV+          +E    V F DVAG+ + +LEL E+
Sbjct: 133 QSGPGSQ------AMNFGKSKARVQ----------MEPQTQVTFGDVAGIEQAKLELTEV 192

Query: 433 VKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFSISASQFVEIYVG 492
           V F    + +   G KIP G+LL GPPG GKTLLAKAVAGEAGV FFSIS S+FVE++VG
Sbjct: 193 VDFLKNADRFTELGAKIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSISGSEFVEMFVG 252

Query: 493 VGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERDATLNQLLVCLDGFEG 552
           VGASRVR L+++AK NAP +VFIDE+DAVGR+RG   G G  ER+ TLNQLL  +DGFEG
Sbjct: 253 VGASRVRDLFEQAKANAPCIVFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLTEMDGFEG 312

Query: 553 RGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILKVHARKKPMAEDVDYM 612
              +I +A+TNRPD+LD AL+RPGRFDR++ + +P   GR EIL VHAR K +++DVD  
Sbjct: 313 NTGIIIVAATNRPDVLDSALMRPGRFDRQVVVDRPDYAGRREILNVHARGKTLSQDVDLD 372

Query: 613 AVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIEERGMLDGKERSPETW 672
            +A  T G  GA+L+N++  AA+   R   TEI+ D++  A       +L G E+     
Sbjct: 373 KIARRTPGFTGADLSNLLNEAAILAARRNLTEISMDEVNDAID----RVLAGPEKKNRVM 432

Query: 673 KQ-----VAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYV-------RMKMNAINR 732
            +     VA +EA  A+V    PD   ++ ++I PR GR  G         RM+    +R
Sbjct: 433 SEKRKTLVAYHEAGHALVGALMPDYDPVQKISIIPR-GRAGGLTWFTPSEDRMESGLYSR 492

Query: 733 QSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSSSDDFSGDKTD 792
             L + + V L  R A+E+  GE+++                     G+S+D        
Sbjct: 493 SYLQNQMAVALGGRIAEEIIFGEEEVTT-------------------GASND-------- 552

Query: 793 DIDQEDIDGEEDDMLSTIWAETADNARSAARTF----VLGGLSEKHHGVSNFWVADRIND 852
                               + A  AR     F     LG ++    G   F   D  +D
Sbjct: 553 ------------------LQQVARVARQMVTRFGMSDRLGPVALGRQGGGVFLGRDIASD 605

Query: 853 ----------IDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLV 886
                     ID E  ++++  Y RAK++L +NR ++D + + L++K+++  +E   L+
Sbjct: 613 RDFSDETAAAIDEEVSQLVDQAYQRAKQVLVENRGILDQLAEILVEKETVDSEELQTLL 605

BLAST of HG10023539 vs. ExPASy TrEMBL
Match: A0A6J1D002 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015799 PE=3 SV=1)

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 809/941 (85.97%), Postives = 836/941 (88.84%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER   L      SSPFPNSRLGILK R WRRPY SISSQIS PS  +  E  DSKKN
Sbjct: 1   MACERFHNLSSSIYSSSPFPNSRLGILKQRRWRRPYPSISSQISTPSNFRGDEDEDSKKN 60

Query: 61  NL-NLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWS 120
            L N L+LSVTLTI+S S PISSA AATA+KEVKERRRG KKSS KK+EALSPQELLSWS
Sbjct: 61  RLKNFLKLSVTLTIVSASFPISSAHAATAAKEVKERRRGTKKSSAKKIEALSPQELLSWS 120

Query: 121 QGLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVE 180
           QGLP VSNRIPYTE+LDLKREGKVKHVI+VP GF R RSEPVMVVLEDSRVLRTVLPS E
Sbjct: 121 QGLPPVSNRIPYTEILDLKREGKVKHVIRVPYGFFRQRSEPVMVVLEDSRVLRTVLPSGE 180

Query: 181 SNRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAA 240
           +NRRFWELWDELGID+VCVNAYTPPIKPPD+P PYLGFL RVP FMYSFVKPKKESKRAA
Sbjct: 181 NNRRFWELWDELGIDTVCVNAYTPPIKPPDLPAPYLGFLLRVPAFMYSFVKPKKESKRAA 240

Query: 241 ELRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARK 300
           E+RRVREELKMEKTVELAKMRQEREMIEKA+KMQKK+EERRIKR+TRRKK+EESLREARK
Sbjct: 241 EIRRVREELKMEKTVELAKMRQEREMIEKALKMQKKQEERRIKRQTRRKKEEESLREARK 300

Query: 301 NYQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK 360
           NYQHMAIVW NL+RDSNVAT LGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK
Sbjct: 301 NYQHMAIVWTNLSRDSNVATTLGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERK 360

Query: 361 KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN 420
           KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN
Sbjct: 361 KMRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVN 420

Query: 421 VKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE 480
           VKFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE
Sbjct: 421 VKFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE 480

Query: 481 AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG 540
           AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG
Sbjct: 481 AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG 540

Query: 541 QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRM 600
           QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+
Sbjct: 541 QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRI 600

Query: 601 EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQA 660
           EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAA+NMIRD RTEITTDDLLQA
Sbjct: 601 EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAAINMIRDSRTEITTDDLLQA 660

Query: 661 AQIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM 720
           AQIEERGMLD KERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM
Sbjct: 661 AQIEERGMLDRKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRM 720

Query: 721 KMNAI-------NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNK 780
           KMNAI        RQSLLDHITVQLAPRAADELW+G  Q                     
Sbjct: 721 KMNAIKYNEGMLTRQSLLDHITVQLAPRAADELWYGAHQ--------------------- 780

Query: 781 MGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVS 840
                                       LSTIWAETADNARSAARTFVLGGLSEKH+GVS
Sbjct: 781 ----------------------------LSTIWAETADNARSAARTFVLGGLSEKHYGVS 840

Query: 841 NFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVE 900
           NFWVADRINDID+EALRILNVCY+RAKEILQQNR LMDAVVDDLIQKKSLTK+EF  LVE
Sbjct: 841 NFWVADRINDIDLEALRILNVCYERAKEILQQNRTLMDAVVDDLIQKKSLTKREFFHLVE 892

Query: 901 LHGSVKPMPLSIIDLRIAKRTKFEEEMMKKNQKKIPVGSNS 928
           LHGS+KPMP SIIDLRIAKRTKF+EEMMK NQKKI +GS+S
Sbjct: 901 LHGSIKPMPPSIIDLRIAKRTKFQEEMMKINQKKIAIGSSS 892

BLAST of HG10023539 vs. ExPASy TrEMBL
Match: A0A6J1JSL5 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488543 PE=3 SV=1)

HSP 1 Score: 1450.3 bits (3753), Expect = 0.0e+00
Identity = 779/942 (82.70%), Postives = 823/942 (87.37%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER  T       SSPFPNSRL ILKPRTWRRPY S+SSQIS PS SQS E NDSKKN
Sbjct: 1   MACERFHTFSSSIYSSSPFPNSRLRILKPRTWRRPYPSVSSQISSPSNSQSDEDNDSKKN 60

Query: 61  NLNLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQ 120
            LNLL+LSVTLT++STSL  S+ALAATASKEVK RRRG KKSS KK +ALSPQELLSWSQ
Sbjct: 61  KLNLLKLSVTLTVVSTSLHTSNALAATASKEVKGRRRGAKKSSTKKGDALSPQELLSWSQ 120

Query: 121 GLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVES 180
           GLPSVSNRIPYTE+LDLKREGKVKHVIKVPNGFLR+R EPVMVVLEDSRVLRTVLPSVES
Sbjct: 121 GLPSVSNRIPYTELLDLKREGKVKHVIKVPNGFLRLRPEPVMVVLEDSRVLRTVLPSVES 180

Query: 181 NRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAE 240
           N RFW LWDELGIDSVCVNAYTPP+K PDVPTPYLGFL RVP F+YSFVKPKKESKRAAE
Sbjct: 181 NGRFWVLWDELGIDSVCVNAYTPPVKLPDVPTPYLGFLTRVPAFLYSFVKPKKESKRAAE 240

Query: 241 LRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKN 300
           +RR+REE+KMEKT  L KMRQEREMIEK +KMQKKEE+ RI+RE  RKK+E+SL EAR N
Sbjct: 241 VRRIREEIKMEKTAALEKMRQEREMIEKTVKMQKKEEDMRIRREANRKKREQSLNEARIN 300

Query: 301 YQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKK 360
           YQ MA  WA LARD NV +ALG +FFVIFY+TVV SYRRQKKDYEDRLKIEKAEAEERKK
Sbjct: 301 YQRMANFWAELARDPNVVSALGFLFFVIFYQTVVLSYRRQKKDYEDRLKIEKAEAEERKK 360

Query: 361 MRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNV 420
           MR+LERE+EG+EGEDDD+E GKGEQNPYLKMA QFMKSGARVRR+HGKR  QYLERG+NV
Sbjct: 361 MRDLEREMEGLEGEDDDVEPGKGEQNPYLKMAAQFMKSGARVRRSHGKRTLQYLERGMNV 420

Query: 421 KFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480
           KFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA
Sbjct: 421 KFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480

Query: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQ 540
           GVNFFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQ
Sbjct: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQ 540

Query: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRME 600
           ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+E
Sbjct: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRLE 600

Query: 601 ILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660
           ILKVHARKKPMA D+DYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA
Sbjct: 601 ILKVHARKKPMANDLDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660

Query: 661 QIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMK 720
           QIEERGMLD KERS ETWKQVA+NEAAMAVVA+NFPDLKNIEFVTIAPRAGRELGYVRMK
Sbjct: 661 QIEERGMLDKKERSLETWKQVALNEAAMAVVAMNFPDLKNIEFVTIAPRAGRELGYVRMK 720

Query: 721 MNA-------INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKM 780
           M+A       + RQSLLDHITVQLAPRAADELW+GEDQ                      
Sbjct: 721 MDAMRFNEGMLTRQSLLDHITVQLAPRAADELWYGEDQ---------------------- 780

Query: 781 GSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSN 840
                                      LSTIW ETADNARSAARTFVLGGLSEKH+GVSN
Sbjct: 781 ---------------------------LSTIWVETADNARSAARTFVLGGLSEKHYGVSN 840

Query: 841 FWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVEL 900
           FWVADRINDID+EALRIL+VCY+RAKEILQQNRKLMD V+DDLIQKKSLTK EFL LVEL
Sbjct: 841 FWVADRINDIDLEALRILHVCYERAKEILQQNRKLMDVVIDDLIQKKSLTKNEFLHLVEL 893

Query: 901 HGSVKPMPLSIIDLRIAKRTKF-EEEMMKKNQKKIPVGSNSS 929
           HGS+KPMP SIIDLRIAKRTK  EEE MKKNQKKI VGS+++
Sbjct: 901 HGSIKPMPPSIIDLRIAKRTKLQEEEKMKKNQKKIAVGSSNT 893

BLAST of HG10023539 vs. ExPASy TrEMBL
Match: A0A6J1JNT1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488543 PE=3 SV=1)

HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 776/935 (82.99%), Postives = 817/935 (87.38%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER  T       SSPFPNSRL ILKPRTWRRPY S+SSQIS PS SQS E NDSKKN
Sbjct: 1   MACERFHTFSSSIYSSSPFPNSRLRILKPRTWRRPYPSVSSQISSPSNSQSDEDNDSKKN 60

Query: 61  NLNLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQ 120
            LNLL+LSVTLT++STSL  S+ALAATASKEVK RRRG KKSS KK +ALSPQELLSWSQ
Sbjct: 61  KLNLLKLSVTLTVVSTSLHTSNALAATASKEVKGRRRGAKKSSTKKGDALSPQELLSWSQ 120

Query: 121 GLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVES 180
           GLPSVSNRIPYTE+LDLKREGKVKHVIKVPNGFLR+R EPVMVVLEDSRVLRTVLPSVES
Sbjct: 121 GLPSVSNRIPYTELLDLKREGKVKHVIKVPNGFLRLRPEPVMVVLEDSRVLRTVLPSVES 180

Query: 181 NRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAE 240
           N RFW LWDELGIDSVCVNAYTPP+K PDVPTPYLGFL RVP F+YSFVKPKKESKRAAE
Sbjct: 181 NGRFWVLWDELGIDSVCVNAYTPPVKLPDVPTPYLGFLTRVPAFLYSFVKPKKESKRAAE 240

Query: 241 LRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKN 300
           +RR+REE+KMEKT  L KMRQEREMIEK +KMQKKEE+ RI+RE  RKK+E+SL EAR N
Sbjct: 241 VRRIREEIKMEKTAALEKMRQEREMIEKTVKMQKKEEDMRIRREANRKKREQSLNEARIN 300

Query: 301 YQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKK 360
           YQ MA  WA LARD NV +ALG +FFVIFY+TVV SYRRQKKDYEDRLKIEKAEAEERKK
Sbjct: 301 YQRMANFWAELARDPNVVSALGFLFFVIFYQTVVLSYRRQKKDYEDRLKIEKAEAEERKK 360

Query: 361 MRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNV 420
           MR+LERE+EG+EGEDDD+E GKGEQNPYLKMA QFMKSGARVRR+HGKR  QYLERG+NV
Sbjct: 361 MRDLEREMEGLEGEDDDVEPGKGEQNPYLKMAAQFMKSGARVRRSHGKRTLQYLERGMNV 420

Query: 421 KFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480
           KFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA
Sbjct: 421 KFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480

Query: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQ 540
           GVNFFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQ
Sbjct: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQ 540

Query: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRME 600
           ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+E
Sbjct: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRLE 600

Query: 601 ILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660
           ILKVHARKKPMA D+DYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA
Sbjct: 601 ILKVHARKKPMANDLDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660

Query: 661 QIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMK 720
           QIEERGMLD KERS ETWKQVA+NEAAMAVVA+NFPDLKNIEFVTIAPRAGRELGYVRMK
Sbjct: 661 QIEERGMLDKKERSLETWKQVALNEAAMAVVAMNFPDLKNIEFVTIAPRAGRELGYVRMK 720

Query: 721 MNA-------INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKM 780
           M+A       + RQSLLDHITVQLAPRAADELW+GEDQ                      
Sbjct: 721 MDAMRFNEGMLTRQSLLDHITVQLAPRAADELWYGEDQ---------------------- 780

Query: 781 GSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSN 840
                                      LSTIW ETADNARSAARTFVLGGLSEKH+GVSN
Sbjct: 781 ---------------------------LSTIWVETADNARSAARTFVLGGLSEKHYGVSN 840

Query: 841 FWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVEL 900
           FWVADRINDID+EALRIL+VCY+RAKEILQQNRKLMD V+DDLIQKKSLTK EFL LVEL
Sbjct: 841 FWVADRINDIDLEALRILHVCYERAKEILQQNRKLMDVVIDDLIQKKSLTKNEFLHLVEL 886

Query: 901 HGSVKPMPLSIIDLRIAKRTKF-EEEMMKKNQKKI 922
           HGS+KPMP SIIDLRIAKRTK  EEE MKKNQKKI
Sbjct: 901 HGSIKPMPPSIIDLRIAKRTKLQEEEKMKKNQKKI 886

BLAST of HG10023539 vs. ExPASy TrEMBL
Match: A0A6J1GQ76 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456101 PE=3 SV=1)

HSP 1 Score: 1438.7 bits (3723), Expect = 0.0e+00
Identity = 774/942 (82.17%), Postives = 820/942 (87.05%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER  T       SSPFPNSRL I K RTWRRPY S+SSQIS PS SQS E  DSKKN
Sbjct: 1   MACERFHTFSSSIYSSSPFPNSRLRIFKQRTWRRPYPSVSSQISSPSNSQSDEDIDSKKN 60

Query: 61  NLNLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQ 120
            LNLL+LSVTLT++STSL  S+ALAATASKEVKERRRG KKSS KK +ALSPQELLSWSQ
Sbjct: 61  KLNLLKLSVTLTVVSTSLHTSNALAATASKEVKERRRGAKKSSTKKGDALSPQELLSWSQ 120

Query: 121 GLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVES 180
           GLPSVSNRIPYTE+LDLKREGKVKHVIKVPNGFLR+R EPVMVVLEDSRVLRTVLPSVES
Sbjct: 121 GLPSVSNRIPYTELLDLKREGKVKHVIKVPNGFLRLRPEPVMVVLEDSRVLRTVLPSVES 180

Query: 181 NRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAE 240
           N RFW LWDELGIDSVCVNAYTPP+K PDVPTPYLGFL RVP F+YSFVKPKKESKRAAE
Sbjct: 181 NGRFWALWDELGIDSVCVNAYTPPVKLPDVPTPYLGFLTRVPAFLYSFVKPKKESKRAAE 240

Query: 241 LRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKN 300
           +RR+REE+KMEKT  L KMRQEREMIEK +KMQKKEE+ RI+RE   KK+E+SL +AR N
Sbjct: 241 VRRIREEIKMEKTAALEKMRQEREMIEKTVKMQKKEEDMRIRREANIKKREQSLNKARIN 300

Query: 301 YQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKK 360
           YQ MA  WA+LARD NV +ALG +FFVIFY+TVV SYRRQKKDYEDRLKIEKAEAEERKK
Sbjct: 301 YQRMANFWADLARDPNVVSALGFLFFVIFYQTVVLSYRRQKKDYEDRLKIEKAEAEERKK 360

Query: 361 MRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNV 420
           MR+LERE+EG+EGEDDD+E GKGEQNPYLKMA QFMKSGARVRR+HGKR PQYLERG+NV
Sbjct: 361 MRDLEREMEGLEGEDDDVEPGKGEQNPYLKMAAQFMKSGARVRRSHGKRTPQYLERGMNV 420

Query: 421 KFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480
           KFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA
Sbjct: 421 KFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480

Query: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQ 540
           GVNFFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQ
Sbjct: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQ 540

Query: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRME 600
           ERDATLNQLLV LDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+E
Sbjct: 541 ERDATLNQLLVSLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRLE 600

Query: 601 ILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660
           ILKVHARKKPMA D+DYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA
Sbjct: 601 ILKVHARKKPMANDLDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660

Query: 661 QIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMK 720
           QIEERGMLD KERS ETWKQVA+NEAAMAVVA+NFPDLKNIEFVTIAPRAGRELGYVRMK
Sbjct: 661 QIEERGMLDKKERSLETWKQVALNEAAMAVVAMNFPDLKNIEFVTIAPRAGRELGYVRMK 720

Query: 721 MNA-------INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKM 780
           M+A       + RQSLLDHITVQLAPRAADELW+GEDQ                      
Sbjct: 721 MDAMRFNEGMLTRQSLLDHITVQLAPRAADELWYGEDQ---------------------- 780

Query: 781 GSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSN 840
                                      LSTIW ETADNARSAARTFVLGGLSEKH+GVSN
Sbjct: 781 ---------------------------LSTIWVETADNARSAARTFVLGGLSEKHYGVSN 840

Query: 841 FWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVEL 900
           FWVADRINDID+EALRIL+VCY+RAKEILQQNRKLMD V+DDLIQKKSLTK EFL LVEL
Sbjct: 841 FWVADRINDIDLEALRILHVCYERAKEILQQNRKLMDVVIDDLIQKKSLTKNEFLHLVEL 893

Query: 901 HGSVKPMPLSIIDLRIAKRTKF-EEEMMKKNQKKIPVGSNSS 929
           HGS+KP P SIIDLRIAKRTK  EEE MKKNQKKI VGS+++
Sbjct: 901 HGSIKPTPPSIIDLRIAKRTKLQEEEKMKKNQKKIAVGSSNT 893

BLAST of HG10023539 vs. ExPASy TrEMBL
Match: A0A6J1GQ73 (probable inactive ATP-dependent zinc metalloprotease FTSHI 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456101 PE=3 SV=1)

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 771/935 (82.46%), Postives = 814/935 (87.06%), Query Frame = 0

Query: 1   MACERCLTL------SSPFPNSRLGILKPRTWRRPYASISSQISIPSYSQSGEGNDSKKN 60
           MACER  T       SSPFPNSRL I K RTWRRPY S+SSQIS PS SQS E  DSKKN
Sbjct: 1   MACERFHTFSSSIYSSSPFPNSRLRIFKQRTWRRPYPSVSSQISSPSNSQSDEDIDSKKN 60

Query: 61  NLNLLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQ 120
            LNLL+LSVTLT++STSL  S+ALAATASKEVKERRRG KKSS KK +ALSPQELLSWSQ
Sbjct: 61  KLNLLKLSVTLTVVSTSLHTSNALAATASKEVKERRRGAKKSSTKKGDALSPQELLSWSQ 120

Query: 121 GLPSVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVES 180
           GLPSVSNRIPYTE+LDLKREGKVKHVIKVPNGFLR+R EPVMVVLEDSRVLRTVLPSVES
Sbjct: 121 GLPSVSNRIPYTELLDLKREGKVKHVIKVPNGFLRLRPEPVMVVLEDSRVLRTVLPSVES 180

Query: 181 NRRFWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAE 240
           N RFW LWDELGIDSVCVNAYTPP+K PDVPTPYLGFL RVP F+YSFVKPKKESKRAAE
Sbjct: 181 NGRFWALWDELGIDSVCVNAYTPPVKLPDVPTPYLGFLTRVPAFLYSFVKPKKESKRAAE 240

Query: 241 LRRVREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKN 300
           +RR+REE+KMEKT  L KMRQEREMIEK +KMQKKEE+ RI+RE   KK+E+SL +AR N
Sbjct: 241 VRRIREEIKMEKTAALEKMRQEREMIEKTVKMQKKEEDMRIRREANIKKREQSLNKARIN 300

Query: 301 YQHMAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKK 360
           YQ MA  WA+LARD NV +ALG +FFVIFY+TVV SYRRQKKDYEDRLKIEKAEAEERKK
Sbjct: 301 YQRMANFWADLARDPNVVSALGFLFFVIFYQTVVLSYRRQKKDYEDRLKIEKAEAEERKK 360

Query: 361 MRELERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNV 420
           MR+LERE+EG+EGEDDD+E GKGEQNPYLKMA QFMKSGARVRR+HGKR PQYLERG+NV
Sbjct: 361 MRDLEREMEGLEGEDDDVEPGKGEQNPYLKMAAQFMKSGARVRRSHGKRTPQYLERGMNV 420

Query: 421 KFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480
           KFEDVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA
Sbjct: 421 KFEDVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEA 480

Query: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQ 540
           GVNFFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQ
Sbjct: 481 GVNFFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQ 540

Query: 541 ERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRME 600
           ERDATLNQLLV LDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGR+E
Sbjct: 541 ERDATLNQLLVSLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRLE 600

Query: 601 ILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660
           ILKVHARKKPMA D+DYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA
Sbjct: 601 ILKVHARKKPMANDLDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAA 660

Query: 661 QIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMK 720
           QIEERGMLD KERS ETWKQVA+NEAAMAVVA+NFPDLKNIEFVTIAPRAGRELGYVRMK
Sbjct: 661 QIEERGMLDKKERSLETWKQVALNEAAMAVVAMNFPDLKNIEFVTIAPRAGRELGYVRMK 720

Query: 721 MNA-------INRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKM 780
           M+A       + RQSLLDHITVQLAPRAADELW+GEDQ                      
Sbjct: 721 MDAMRFNEGMLTRQSLLDHITVQLAPRAADELWYGEDQ---------------------- 780

Query: 781 GSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSN 840
                                      LSTIW ETADNARSAARTFVLGGLSEKH+GVSN
Sbjct: 781 ---------------------------LSTIWVETADNARSAARTFVLGGLSEKHYGVSN 840

Query: 841 FWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVEL 900
           FWVADRINDID+EALRIL+VCY+RAKEILQQNRKLMD V+DDLIQKKSLTK EFL LVEL
Sbjct: 841 FWVADRINDIDLEALRILHVCYERAKEILQQNRKLMDVVIDDLIQKKSLTKNEFLHLVEL 886

Query: 901 HGSVKPMPLSIIDLRIAKRTKF-EEEMMKKNQKKI 922
           HGS+KP P SIIDLRIAKRTK  EEE MKKNQKKI
Sbjct: 901 HGSIKPTPPSIIDLRIAKRTKLQEEEKMKKNQKKI 886

BLAST of HG10023539 vs. TAIR 10
Match: AT3G16290.1 (AAA-type ATPase family protein )

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 654/925 (70.70%), Postives = 752/925 (81.30%), Query Frame = 0

Query: 1   MACERCLTLSSPFPNSRLGILKPRTWRRPYASISSQISIPS--YSQSGEGND-SKKNNLN 60
           MAC     L S  P+  L     +   R Y SIS Q +  +    + G+ ND +K N +N
Sbjct: 1   MACR--FPLHSSSPSQFLSPENRQRLPRNYPSISCQNNSATNVVHEDGDDNDKAKTNQVN 60

Query: 61  LLELSVTLTILSTSLPISSALAATASKEVKERRRGPKKSSVKKVEALSPQELLSWSQGLP 120
           LL + +TLTI+S SL    A  + A+ +V ER+R  KK      EAL+ ++L +WS+ LP
Sbjct: 61  LLAIPITLTIISASL----AKPSFAAAKVTERKRTQKKPQ----EALTLEQLKAWSKDLP 120

Query: 121 SVSNRIPYTEVLDLKREGKVKHVIKVPNGFLRMRSEPVMVVLEDSRVLRTVLPSVESNRR 180
            VSNRIPYT++L LK EGK+KHVIK PN  LR ++EPV+VVLEDSRVLRTVLPS+E N+R
Sbjct: 121 VVSNRIPYTDILSLKAEGKLKHVIKPPNLSLRQKAEPVLVVLEDSRVLRTVLPSLEGNKR 180

Query: 181 FWELWDELGIDSVCVNAYTPPIKPPDVPTPYLGFLARVPEFMYSFVKPKKESKRAAELRR 240
           FWE WDELGID  CVNAYTPP+K P VP+PYLGFL +VP +M ++VKPKKESKRAAEL+R
Sbjct: 181 FWEQWDELGIDVQCVNAYTPPVKRPPVPSPYLGFLWKVPAYMLTWVKPKKESKRAAELKR 240

Query: 241 VREELKMEKTVELAKMRQEREMIEKAMKMQKKEEERRIKRETRRKKQEESLREARKNYQH 300
           +RE+ K ++  E+  M++ER M+EK MK QKK++ER+ ++  R+KK EESLREARKNY+ 
Sbjct: 241 MREDFKRQRKEEIETMKEERVMMEKTMKAQKKQQERKKRKAVRKKKYEESLREARKNYRD 300

Query: 301 MAIVWANLARDSNVATALGLVFFVIFYRTVVFSYRRQKKDYEDRLKIEKAEAEERKKMRE 360
           MA +WA LA+D NVATALGLVFF IFYR VV +YR+QKKDYEDRLKIEKAEA+ERKKMRE
Sbjct: 301 MADMWARLAQDPNVATALGLVFFYIFYRVVVLNYRKQKKDYEDRLKIEKAEADERKKMRE 360

Query: 361 LERELEGIEGEDDDIEQGKGEQNPYLKMAMQFMKSGARVRRAHGKRLPQYLERGVNVKFE 420
           LERE+EGIE ED+++E+G GE+NPYL+MAMQFMKSGARVRRA  KRLP+YLERGV+VKF 
Sbjct: 361 LEREMEGIEEEDEEVEEGTGEKNPYLQMAMQFMKSGARVRRASNKRLPEYLERGVDVKFT 420

Query: 421 DVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN 480
           DVAGLGKIRLELEEIVKFFT GEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN
Sbjct: 421 DVAGLGKIRLELEEIVKFFTHGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVN 480

Query: 481 FFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGGQERD 540
           FFSISASQFVEIYVGVGASRVRALYQEA+ENAPSVVFIDELDAVGRERGLIKGSGGQERD
Sbjct: 481 FFSISASQFVEIYVGVGASRVRALYQEARENAPSVVFIDELDAVGRERGLIKGSGGQERD 540

Query: 541 ATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRMEILK 600
           ATLNQLLV LDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKI+IPKPGLIGRMEIL+
Sbjct: 541 ATLNQLLVSLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIFIPKPGLIGRMEILQ 600

Query: 601 VHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQAAQIE 660
           VHARKKPMAED+DYMAVASMTDGMVGAELANIVE+AA+NM+RDGRTE+TTDDLLQAAQIE
Sbjct: 601 VHARKKPMAEDLDYMAVASMTDGMVGAELANIVEIAAINMMRDGRTELTTDDLLQAAQIE 660

Query: 661 ERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGRELGYVRMKMNA 720
           ERGMLD K+RS ETW+QVAINEAAMAVVAVNFPD+KNIEF+TI PRAGRELGYVR+KM+ 
Sbjct: 661 ERGMLDRKDRSLETWRQVAINEAAMAVVAVNFPDMKNIEFLTINPRAGRELGYVRVKMDH 720

Query: 721 I-------NRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWPKIYNKMGSS 780
           I       +RQS+LDHITVQLAPRAADELW+GEDQ                         
Sbjct: 721 IKFKEGMLSRQSILDHITVQLAPRAADELWYGEDQ------------------------- 780

Query: 781 SDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGLSEKHHGVSNFWV 840
                                   LSTIWAET+DNARSAAR+ VLGGLS+KHHG++NFWV
Sbjct: 781 ------------------------LSTIWAETSDNARSAARSLVLGGLSDKHHGLNNFWV 840

Query: 841 ADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQEFLQLVELHGS 900
           ADRINDID+EALRILN+CY+RAKEIL +NR LMD VV+ L+QKKSLTKQEF  LVEL+GS
Sbjct: 841 ADRINDIDVEALRILNMCYERAKEILGRNRTLMDEVVEKLVQKKSLTKQEFFTLVELYGS 866

Query: 901 VKPMPLSIIDLRIAKRTKFEEEMMK 916
            KPMP SI++LR  KR + EE ++K
Sbjct: 901 SKPMPPSILELRKIKRLELEEMVLK 866

BLAST of HG10023539 vs. TAIR 10
Match: AT5G15250.1 (FTSH protease 6 )

HSP 1 Score: 287.3 bits (734), Expect = 4.3e-77
Identity = 186/484 (38.43%), Postives = 266/484 (54.96%), Query Frame = 0

Query: 408 LERGVNVKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLA 467
           +E    + FEDVAG+ + + + EEIV+F    E +   G KIP G+LL GPPG GKTLLA
Sbjct: 215 MEPNTGITFEDVAGVDEAKQDFEEIVEFLKTPEKFSALGAKIPKGVLLTGPPGTGKTLLA 274

Query: 468 KAVAGEAGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGL 527
           KA+AGEAGV FFS+S S+F+E++VGVGASR R L+ +AK N+P +VFIDE+DAVGR RG 
Sbjct: 275 KAIAGEAGVPFFSLSGSEFIEMFVGVGASRARDLFNKAKANSPCIVFIDEIDAVGRMRGT 334

Query: 528 IKGSGGQERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKP 587
             G G  ER+ TLNQ+L  +DGF G   VI IA+TNRP+ILD AL+RPGRFDR++ +  P
Sbjct: 335 GIGGGNDEREQTLNQILTEMDGFAGNTGVIVIAATNRPEILDSALLRPGRFDRQVSVGLP 394

Query: 588 GLIGRMEILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEIT- 647
            + GR EILKVH+R K + +DV    +A  T G  GA+LAN++  AA+   R G+ +IT 
Sbjct: 395 DIRGREEILKVHSRSKKLDKDVSLSVIAMRTPGFSGADLANLMNEAAILAGRRGKDKITL 454

Query: 648 --TDDLLQ--AAQIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAP 707
              DD +    A +E   M+DGK ++      VA +E   A+ A        ++ VT+ P
Sbjct: 455 TEIDDSIDRIVAGMEGTKMIDGKSKA-----IVAYHEVGHAICATLTEGHDPVQKVTLVP 514

Query: 708 RAGRELGYVRM----KMNAINRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIP 767
           R G+  G            +++Q L   I   L  RAA+++  GE ++    +     + 
Sbjct: 515 R-GQARGLTWFLPGEDPTLVSKQQLFARIVGGLGGRAAEDVIFGEPEITTGAAGDLQQVT 574

Query: 768 DWPKIYNKMGSSSDDFSGDKTDD-IDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGG 827
           +  +    M   S+      TD  + Q D+                       R      
Sbjct: 575 EIARQMVTMFGMSEIGPWALTDPAVKQNDV---------------------VLRMLARNS 634

Query: 828 LSEKHHGVSNFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLT 882
           +SEK              DID    +I+   Y+ AK+ ++ NR+ +D +VD L++K++LT
Sbjct: 635 MSEK-----------LAEDIDSCVKKIIGDAYEVAKKHVRNNREAIDKLVDVLLEKETLT 660

BLAST of HG10023539 vs. TAIR 10
Match: AT1G06430.1 (FTSH protease 8 )

HSP 1 Score: 286.6 bits (732), Expect = 7.3e-77
Identity = 187/504 (37.10%), Postives = 271/504 (53.77%), Query Frame = 0

Query: 408 LERGVNVKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLA 467
           +E    V F+DVAG+ + + +  E+V+F  + E +   G +IP G+LL GPPG GKTLLA
Sbjct: 211 MEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVGPPGTGKTLLA 270

Query: 468 KAVAGEAGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGL 527
           KA+AGEAGV FFSIS S+FVE++VGVGASRVR L+++AKENAP +VF+DE+DAVGR+RG 
Sbjct: 271 KAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGT 330

Query: 528 IKGSGGQERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKP 587
             G G  ER+ TLNQLL  +DGFEG   VI +A+TNR DILD AL+RPGRFDR++ +  P
Sbjct: 331 GIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVP 390

Query: 588 GLIGRMEILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITT 647
            + GR +ILKVH+  K     V    +A  T G  GA+LAN++  AA+   R G+T I++
Sbjct: 391 DVKGRTDILKVHSGNKKFESGVSLEVIAMRTPGFSGADLANLLNEAAILAGRRGKTAISS 450

Query: 648 ---DDLLQ--AAQIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAP 707
              DD +    A +E   M DGK +S      VA +E   A+     P    ++ VT+ P
Sbjct: 451 KEIDDSIDRIVAGMEGTVMTDGKSKS-----LVAYHEVGHAICGTLTPGHDAVQKVTLIP 510

Query: 708 RAGRELGYVRM----KMNAINRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIP 767
           R G+  G            I++Q L   I   L  RAA+E+  GE ++     S      
Sbjct: 511 R-GQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEVIFGESEVTTGAVS------ 570

Query: 768 DWPKIYNKMGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTI-------WA--ETADNARSA 827
                                   D + I G    M++T        W+  ++++ +   
Sbjct: 571 ------------------------DLQQITGLAKQMVTTFGMSEIGPWSLMDSSEQSDVI 630

Query: 828 ARTFVLGGLSEKHHGVSNFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDD 887
            R      +SEK             NDID     + +  Y+ A   ++ NR+ MD +V+ 
Sbjct: 631 MRMMARNSMSEK-----------LANDIDTAVKTLSDKAYEIALSQIRNNREAMDKIVEI 667

Query: 888 LIQKKSLTKQEFLQLVELHGSVKP 894
           L++K++++  EF  ++     + P
Sbjct: 691 LLEKETMSGDEFRAILSEFTEIPP 667

BLAST of HG10023539 vs. TAIR 10
Match: AT5G42270.1 (FtsH extracellular protease family )

HSP 1 Score: 286.6 bits (732), Expect = 7.3e-77
Identity = 183/484 (37.81%), Postives = 271/484 (55.99%), Query Frame = 0

Query: 414 VKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGE 473
           V F DVAG  + +LEL+E+V F    + Y   G KIP G LL GPPG GKTLLA+AVAGE
Sbjct: 247 VTFGDVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGPPGTGKTLLARAVAGE 306

Query: 474 AGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGLIKGSGG 533
           AGV FFS +AS+FVE++VGVGASRVR L+++AK  AP +VFIDE+DAVGR+RG   G G 
Sbjct: 307 AGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEIDAVGRQRGAGMGGGN 366

Query: 534 QERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKPGLIGRM 593
            ER+ T+NQLL  +DGF G   VI +A+TNRPD+LD AL+RPGRFDR++ + +P + GR+
Sbjct: 367 DEREQTINQLLTEMDGFSGNSGVIVLAATNRPDVLDSALLRPGRFDRQVTVDRPDVAGRV 426

Query: 594 EILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITTDDLLQA 653
           +ILKVH+R K + +DVDY  VA  T G  GA+L N++  AA+   R    EI+ D++  A
Sbjct: 427 QILKVHSRGKAIGKDVDYEKVARRTPGFTGADLQNLMNEAAILAARRELKEISKDEISDA 486

Query: 654 AQIEERGMLDGKER-----SPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAPRAGREL 713
            +     ++ G E+     S E  + VA +EA  A+V    P+   +  ++I PR G+  
Sbjct: 487 LE----RIIAGPEKKNAVVSEEKKRLVAYHEAGHALVGALMPEYDPVAKISIIPR-GQAG 546

Query: 714 GYV-------RMKMNAINRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIPDWP 773
           G         R++    +R  L + + V L  R A+E+  G++ +    S+      D+ 
Sbjct: 547 GLTFFAPSEERLESGLYSRSYLENQMAVALGGRVAEEVIFGDENVTTGASN------DFM 606

Query: 774 KIYNKMGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTIWAETADNARSAARTFVLGGL-SE 833
           ++        + F   K   I Q  + G                  +    F+   + S+
Sbjct: 607 QVSRVARQMVERFGFSK--KIGQVAVGG------------------AGGNPFLGQSMSSQ 666

Query: 834 KHHGVSNFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDDLIQKKSLTKQE 885
           K + ++   V      +D E   ++   Y RAKEI+     ++  +   LI+K+++  +E
Sbjct: 667 KDYSMATADV------VDAEVRELVEKAYVRAKEIITTQIDILHKLAQLLIEKETVDGEE 693

BLAST of HG10023539 vs. TAIR 10
Match: AT2G30950.1 (FtsH extracellular protease family )

HSP 1 Score: 285.4 bits (729), Expect = 1.6e-76
Identity = 192/504 (38.10%), Postives = 272/504 (53.97%), Query Frame = 0

Query: 408 LERGVNVKFEDVAGLGKIRLELEEIVKFFTQGEMYRRRGVKIPGGILLCGPPGVGKTLLA 467
           +E    V F+DVAG+ + + +  E+V+F  + E +   G KIP G+LL GPPG GKTLLA
Sbjct: 218 MEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGAKIPKGVLLIGPPGTGKTLLA 277

Query: 468 KAVAGEAGVNFFSISASQFVEIYVGVGASRVRALYQEAKENAPSVVFIDELDAVGRERGL 527
           KA+AGEAGV FFSIS S+FVE++VGVGASRVR L+++AKENAP +VF+DE+DAVGR+RG 
Sbjct: 278 KAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDEIDAVGRQRGT 337

Query: 528 IKGSGGQERDATLNQLLVCLDGFEGRGEVITIASTNRPDILDPALVRPGRFDRKIYIPKP 587
             G G  ER+ TLNQLL  +DGFEG   VI +A+TNR DILD AL+RPGRFDR++ +  P
Sbjct: 338 GIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSALLRPGRFDRQVSVDVP 397

Query: 588 GLIGRMEILKVHARKKPMAEDVDYMAVASMTDGMVGAELANIVEVAALNMIRDGRTEITT 647
            + GR +ILKVHA  K    DV    +A  T G  GA+LAN++  AA+   R  RT I++
Sbjct: 398 DVKGRTDILKVHAGNKKFDNDVSLEIIAMRTPGFSGADLANLLNEAAILAGRRARTSISS 457

Query: 648 ---DDLLQ--AAQIEERGMLDGKERSPETWKQVAINEAAMAVVAVNFPDLKNIEFVTIAP 707
              DD +    A +E   M DGK +S      VA +E   AV     P    ++ VT+ P
Sbjct: 458 KEIDDSIDRIVAGMEGTVMTDGKSKS-----LVAYHEVGHAVCGTLTPGHDAVQKVTLIP 517

Query: 708 RAGRELGYVRM----KMNAINRQSLLDHITVQLAPRAADELWHGEDQMANNRSSTPVGIP 767
           R G+  G            I++Q L   I   L  RAA+E+  G+ ++    ++  VG  
Sbjct: 518 R-GQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEIIFGDSEV----TTGAVG-- 577

Query: 768 DWPKIYNKMGSSSDDFSGDKTDDIDQEDIDGEEDDMLSTI-------WAETADNARS--A 827
                                   D + I G    M++T        W+    +A+S   
Sbjct: 578 ------------------------DLQQITGLARQMVTTFGMSDIGPWSLMDSSAQSDVI 637

Query: 828 ARTFVLGGLSEKHHGVSNFWVADRINDIDMEALRILNVCYDRAKEILQQNRKLMDAVVDD 887
            R      +SEK              DID    ++ +  Y+ A   ++ NR+ MD +V+ 
Sbjct: 638 MRMMARNSMSEK-----------LAEDIDSAVKKLSDSAYEIALSHIKNNREAMDKLVEV 674

Query: 888 LIQKKSLTKQEFLQLVELHGSVKP 894
           L++K+++   EF  ++     + P
Sbjct: 698 LLEKETIGGDEFRAILSEFTEIPP 674