HG10023477 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023477
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionProtein kinase
LocationChr05: 34550114 .. 34554408 (+)
RNA-Seq ExpressionHG10023477
SyntenyHG10023477
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTGCAGAAATTTCCTTTCAAATTGGGACTCTGATGGGGTTTCGATTACTGTTTCTGCTGTTCCAAATTTGTTTTGCTGTTAGATCTTTCTCTATCTGTGAAGCTGCAGGTAATGGGTTTGGTTTGGTTCTGTAATTTTTGAATTGTGTTTGATCTGATCATATAAGTTTCACAAATATTGATTTGGAGTTGAATTTGGATCTGGGTGTGATCTGAAAAGAGTTGGGATTCTGTTAATTGATCACTTTTCTAGGGAAAAAAATCAGTATGATTAGCTTCATGCATAGACTGTTTTCCTTTCCTTTATATTCACCAAGTTGGATGGTTCAGAAATTAGAAGAAGAATTGAGATGCTAATCACTAGCTGATTTTTAGCTTGCTTTCAAGACTGGTAAAATCCAACACATTTCTTATCATGGTTCTCTCTTTCATCAAAGTAGCCACAAAAGCTTCAAGCTGGAAATCACCAAAACAAGCATAATTTACTTCATATCATGTTGCTTGCAAAGGTTTTGTTCCATTTTCTGATTTTACTGCTCTCTTTTTGCAGCTATTCCCCCAGGCAGGACGAGCGGATCGCATTTTGTAATCTCAGGTGAACTTATTTAACCCTTCCTCACCAATTGGATCTTCAAAACTCGTTAAGAATTTAAGTAAAACTAGCAACTTCCAATCTATATCTTCTATATGAAATTAATATGGTTTTATGAATGATATACAGTGAAATCAAGCACCGAAAAAAGTATTGGAAAACATAAATCTCAAGCTAAATGAAAGCTAATTGTGTTGAGTGGTTTTGTGTCCTTATAATGCCACAGACCATGCTGTCACTCCAACATCTCATGGCTTTGGCAGGAAACACCATGGCAATTTGGATAATGCAGCCCCTAGCAAACCATTTAAGAGTTCATCTCCTCAGATCCACTCTCATTATAAAGGTAATTGGGTGTACATTTTAGTTTCTATGTTCTTTCTCTCAAGTTTTTGAGCTATCAAGTAGAAATTCTTGACAGAGATAACATAAACAGTAACATTTGCTTTTCTTCTGATGTAGCCATTCAATCAGATGATTCAAGTAGAGCTCCTAGCATTGCACTTCCGCCTACCGCACCTGCGAAGAAATGGGGGCATGAACATACATTCTCACCATTGACCTCTTCTCATAAATTTCGCCATTCAAGAAGGAAGTTCAGAAACAATGCTCCACAACCTACTTATCGTGCATCGCCCCCGACTAGCAGCCAGAAAGGTTGAGAGTCTCGAGATATACTAGTTCATCTCTTGAATCTTCGCCATTTAGATCTATAGAAGTATTCACTACTGAACTTTCAGGCCCTGCTGCTGTCTCTCCAATTCAGTCACTGTTGCCATCTGTAGTGAGAGGGAGGTCGCCCGGACCTGCGCCATCACCAACGATTCAACCCAACCACTATTATAGTCTGTAAAAGAGAACCTGAACTTTTGTTTAAACCTTGAACACTATTTCATTCATTTACATTGCACAAGGATGCTGTTAACTTTATGGTATGGACATGTTTTCTTTCCAACAGTGCCCATTCCTGCACCTACAACATCTCCTATGGGTTCTTACAAGAAGAAGAAGAGTATGCCACCATCACAAGTTATGATGCTACCACCTCCACCTCCTAATGGGGGTAGGCACAAACTTAACTCTTAGTAGTGCATATCAAATTATCTGAAAATTAACCACATTATAATAACCTTGTGCTTTGCATTCGACACATGTAGATTGTACAATAGCATGTACAGAGCCATTGACATATACGCCTCCCGGGACGCCATGTGGCTGCGTGTGGCCAATTCAAGTCAAAATCATTCTTGATGTTGCAGTATACACATTTTTCCCTCTTGTTTCAGAACTTGCAGAGGAAATTGCTGACGGTATCAGTCTGAACCAAAGCCAAGTTCGAATTATGGGAGCTGATGCTGCTAGTCAGCAGTTAGAAAAAACAACTGTCCTCATCAACTTGGTTCCAAAAGGATCAAAATTCAATCATAACACGGCCTTTTTTATATATCAGAAATTTTGGCGGAGAAAGATATCCATCAACCCTTCTTTGTTTGGAAGATATCAAGTACTTAATGTCAAATATCCAGGTTATCTCTTGATCCTTGATCTTTTGGTTTTGAATATTAAAAAATTTCAACACAGAGTGGATGTGGACAAGTGAATCTAGTGCAGTTGGAACAATCTGAGGGGCATACTTTTTATGTCCTCGGAGTTCCTTCCATTTTTGTCTGAGCAGTTTCAAGAGGGCTTCCTAAAATTCAACAATGTTTTTTTTCCTGATAAGCTCTAGCTCATGCAGGTCTTCCGCCATCTCCTCCGTTAGCGCCTTCAGGTACTTCATCCATTAATGATGGTTTGAACACTAGCAATACTAATGCTGGAAATGCAATTAAACCACTTGGAGTTGACGTGCCAAGACGAAAAAAAGAAGGGCTTGGCAGAAACATGATCGCGGTTATTACTATATCATCGTTCACTGCCCTTGTCATGTGTGTCGGGCTCGCTTGGCTTTGCCTGTTGAGATGCAGAGTGTCTACACATCAACCAGCACAAATTCCACAAAACTTGATAGCCTCACCTACAAAACCACCAGGTAAATCTAATTCAACCAAATTCAGAAATTCTTCTGATTGACATTCCCTTCAAAGTGTCTGCAGTCCTGAACTCAGTCTAGAACTTGGTCTTATTTTCTTGGTATGAGCAATTTCTACAGGCACTGCTGGATTGATAATGGTTGGAAGTGAACCTGGTTCTTCATCCATGCCTCTCGATGCCGATCCAATGACGTATATTGGAGCTGCAAAGAACTTCACCTTGAAAGATATGGAGAAAGCCACTGACAACTTCAATAGTGCAAGAATATTAGGAGAAGGTGGCTTTGGAATTGTTTACAGTGGCAGTTTAGATGATGGAAGGGAAGTGGCTGTGAAGGTTCTTAAACGACATAATCAACATGGTATTCGGGAATTCTTGGCAGAAGTCGAGATGCTGAGCCGTTTACACCATAGAAATCTAGTTAAGTTGATTGGTATCTGTACAGAAGATCAGATTCGCTGCCTGGTATATGAATTGGTTCCTAATGGAAGTGTCGAATCGCATTTACATGGTAAGCAACTTCCTGCAAAATCTTAAGTACCTTGTCATTTGTTGTTTTTTTAAAACAGGCTTAAAAGTACCCATCCTAGATGGTTTGGTATTCATATTCAACTTGTCTTGTTTGATTCCAGGCATTGACAAGTTAACCAGTCCACTTGACTGGGATGCCCGCATGAAGATTGCCCTCGGTGCTGCTCGAGGCCTAGCCTATCTGCATGAAGACTCCAATCCTCGAGTTATTCATCGAGACTTCAAAGCTAGCAACATCTTGTTGGAATATGACTTTACACCAAAAGTTTCAGATTTCGGATTAGCTAGAACTGCACTAGAGGAAGGAAACAAGCACATCTCAACACATGTTATGGGAACTTTTGGGTAAAAAATATCTTTCTATCATGTAGTTCCTTCAAATTCATTTACTTTTCTCAACTTCATCATAAGTTAATCTTATACAATATATTAACTTCTGGTTTCCAGTTATCTAGCTCCAGAATATGCAATGACTGGCCACCTTCTTGTAAAGAGCGATGTCTATAGCTATGGTGTTGTCCTTCTTGAGCTACTTACAGGAAGAAAGCCAGTAGACTTGTCGCTTCCACCCGGACAAGAAAATCTCGTTGCTTGGGCTCGTCCACTTCTCACGAGCAAGGAAGGGTTAGATGCAATAACAGACCCAGCTATTAAGCCTGATATCTCCATTGATAGCTTGGCCAGAGTAGCAGCAATTGCTTCTATGTGTGTGCAACCGGAAGTATCTCACAGACCATTTATGGGTGAGGTTGTTCAAGCCCTGAAACTCGTTTGCAATGAGTTCGAAGAAACTAACGATCCAGTTTCAAGAAGTTACAGCCGAGACGAACTTCTAAGTTACATGGACAGTAAATTTGGAGGAATTTCCGGTGAAATACTAAACGCTCCCGAGACCTCGCACACCTTTCCTTCGGGAAAAGAGACAAATGTAGGATTATCGGCATCAGATTTGATTAGTGCATCTGCAAGATTTGAAGGACAGGAACTGGTTTCTTCTAGATGGCATTCATCAAACTCGGAACCATTGAGAACTGGAAGAAAAAAGCACTTATGGCAGAAGGTAAGAAGTTTGTCACGAGGCAGCTTCAGTGAACATGGCTTTTCTGCAAAGTTTTGGCCTGGATTTCGTTAG

mRNA sequence

ATGAAAACTGCAGAAATTTCCTTTCAAATTGGGACTCTGATGGGGTTTCGATTACTGTTTCTGCTGTTCCAAATTTGTTTTGCTGTTAGATCTTTCTCTATCTGTGAAGCTGCAGACCATGCTGTCACTCCAACATCTCATGGCTTTGGCAGGAAACACCATGGCAATTTGGATAATGCAGCCCCTAGCAAACCATTTAAGAGTTCATCTCCTCAGATCCACTCTCATTATAAAGCCATTCAATCAGATGATTCAAGTAGAGCTCCTAGCATTGCACTTCCGCCTACCGCACCTGCGAAGAAATGGGGGCATGAACATACATTCTCACCATTGACCTCTTCTCATAAATTTCGCCATTCAAGAAGGAAGTTCAGAAACAATGCTCCACAACCTACTTATCGTGCATCGCCCCCGACTAGCAGCCAGAAAGGCCCTGCTGCTGTCTCTCCAATTCAGTCACTGTTGCCATCTGTAGTGAGAGGGAGGTCGCCCGGACCTGCGCCATCACCAACGATTCAACCCAACCACTATTATATGCCCATTCCTGCACCTACAACATCTCCTATGGGTTCTTACAAGAAGAAGAAGAGTATGCCACCATCACAAGTTATGATGCTACCACCTCCACCTCCTAATGGGGATTGTACAATAGCATGTACAGAGCCATTGACATATACGCCTCCCGGGACGCCATGTGGCTGCGTGTGGCCAATTCAAGTCAAAATCATTCTTGATGTTGCAGTATACACATTTTTCCCTCTTGTTTCAGAACTTGCAGAGGAAATTGCTGACGGTATCAGTCTGAACCAAAGCCAAGTTCGAATTATGGGAGCTGATGCTGCTAGTCAGCAGTTAGAAAAAACAACTGTCCTCATCAACTTGGTTCCAAAAGGATCAAAATTCAATCATAACACGGCCTTTTTTATATATCAGAAATTTTGGCGGAGAAAGATATCCATCAACCCTTCTTTGTTTGGAAGATATCAAGTACTTAATGTCAAATATCCAGGTCTTCCGCCATCTCCTCCGTTAGCGCCTTCAGGTACTTCATCCATTAATGATGGTTTGAACACTAGCAATACTAATGCTGGAAATGCAATTAAACCACTTGGAGTTGACGTGCCAAGACGAAAAAAAGAAGGGCTTGGCAGAAACATGATCGCGGTTATTACTATATCATCGTTCACTGCCCTTGTCATGTGTGTCGGGCTCGCTTGGCTTTGCCTGTTGAGATGCAGAGTGTCTACACATCAACCAGCACAAATTCCACAAAACTTGATAGCCTCACCTACAAAACCACCAGGCACTGCTGGATTGATAATGGTTGGAAGTGAACCTGGTTCTTCATCCATGCCTCTCGATGCCGATCCAATGACGTATATTGGAGCTGCAAAGAACTTCACCTTGAAAGATATGGAGAAAGCCACTGACAACTTCAATAGTGCAAGAATATTAGGAGAAGGTGGCTTTGGAATTGTTTACAGTGGCAGTTTAGATGATGGAAGGGAAGTGGCTGTGAAGGTTCTTAAACGACATAATCAACATGGTATTCGGGAATTCTTGGCAGAAGTCGAGATGCTGAGCCGTTTACACCATAGAAATCTAGTTAAGTTGATTGGTATCTGTACAGAAGATCAGATTCGCTGCCTGGTATATGAATTGGTTCCTAATGGAAGTGTCGAATCGCATTTACATGGCATTGACAAGTTAACCAGTCCACTTGACTGGGATGCCCGCATGAAGATTGCCCTCGGTGCTGCTCGAGGCCTAGCCTATCTGCATGAAGACTCCAATCCTCGAGTTATTCATCGAGACTTCAAAGCTAGCAACATCTTGTTGGAATATGACTTTACACCAAAAGTTTCAGATTTCGGATTAGCTAGAACTGCACTAGAGGAAGGAAACAAGCACATCTCAACACATGTTATGGGAACTTTTGGTTATCTAGCTCCAGAATATGCAATGACTGGCCACCTTCTTGTAAAGAGCGATGTCTATAGCTATGGTGTTGTCCTTCTTGAGCTACTTACAGGAAGAAAGCCAGTAGACTTGTCGCTTCCACCCGGACAAGAAAATCTCGTTGCTTGGGCTCGTCCACTTCTCACGAGCAAGGAAGGGTTAGATGCAATAACAGACCCAGCTATTAAGCCTGATATCTCCATTGATAGCTTGGCCAGAGTAGCAGCAATTGCTTCTATGTGTGTGCAACCGGAAGTATCTCACAGACCATTTATGGGTGAGGTTGTTCAAGCCCTGAAACTCGTTTGCAATGAGTTCGAAGAAACTAACGATCCAGTTTCAAGAAGTTACAGCCGAGACGAACTTCTAAGTTACATGGACAGTAAATTTGGAGGAATTTCCGGTGAAATACTAAACGCTCCCGAGACCTCGCACACCTTTCCTTCGGGAAAAGAGACAAATGTAGGATTATCGGCATCAGATTTGATTAGTGCATCTGCAAGATTTGAAGGACAGGAACTGGTTTCTTCTAGATGGCATTCATCAAACTCGGAACCATTGAGAACTGGAAGAAAAAAGCACTTATGGCAGAAGGTAAGAAGTTTGTCACGAGGCAGCTTCAGTGAACATGGCTTTTCTGCAAAGTTTTGGCCTGGATTTCGTTAG

Coding sequence (CDS)

ATGAAAACTGCAGAAATTTCCTTTCAAATTGGGACTCTGATGGGGTTTCGATTACTGTTTCTGCTGTTCCAAATTTGTTTTGCTGTTAGATCTTTCTCTATCTGTGAAGCTGCAGACCATGCTGTCACTCCAACATCTCATGGCTTTGGCAGGAAACACCATGGCAATTTGGATAATGCAGCCCCTAGCAAACCATTTAAGAGTTCATCTCCTCAGATCCACTCTCATTATAAAGCCATTCAATCAGATGATTCAAGTAGAGCTCCTAGCATTGCACTTCCGCCTACCGCACCTGCGAAGAAATGGGGGCATGAACATACATTCTCACCATTGACCTCTTCTCATAAATTTCGCCATTCAAGAAGGAAGTTCAGAAACAATGCTCCACAACCTACTTATCGTGCATCGCCCCCGACTAGCAGCCAGAAAGGCCCTGCTGCTGTCTCTCCAATTCAGTCACTGTTGCCATCTGTAGTGAGAGGGAGGTCGCCCGGACCTGCGCCATCACCAACGATTCAACCCAACCACTATTATATGCCCATTCCTGCACCTACAACATCTCCTATGGGTTCTTACAAGAAGAAGAAGAGTATGCCACCATCACAAGTTATGATGCTACCACCTCCACCTCCTAATGGGGATTGTACAATAGCATGTACAGAGCCATTGACATATACGCCTCCCGGGACGCCATGTGGCTGCGTGTGGCCAATTCAAGTCAAAATCATTCTTGATGTTGCAGTATACACATTTTTCCCTCTTGTTTCAGAACTTGCAGAGGAAATTGCTGACGGTATCAGTCTGAACCAAAGCCAAGTTCGAATTATGGGAGCTGATGCTGCTAGTCAGCAGTTAGAAAAAACAACTGTCCTCATCAACTTGGTTCCAAAAGGATCAAAATTCAATCATAACACGGCCTTTTTTATATATCAGAAATTTTGGCGGAGAAAGATATCCATCAACCCTTCTTTGTTTGGAAGATATCAAGTACTTAATGTCAAATATCCAGGTCTTCCGCCATCTCCTCCGTTAGCGCCTTCAGGTACTTCATCCATTAATGATGGTTTGAACACTAGCAATACTAATGCTGGAAATGCAATTAAACCACTTGGAGTTGACGTGCCAAGACGAAAAAAAGAAGGGCTTGGCAGAAACATGATCGCGGTTATTACTATATCATCGTTCACTGCCCTTGTCATGTGTGTCGGGCTCGCTTGGCTTTGCCTGTTGAGATGCAGAGTGTCTACACATCAACCAGCACAAATTCCACAAAACTTGATAGCCTCACCTACAAAACCACCAGGCACTGCTGGATTGATAATGGTTGGAAGTGAACCTGGTTCTTCATCCATGCCTCTCGATGCCGATCCAATGACGTATATTGGAGCTGCAAAGAACTTCACCTTGAAAGATATGGAGAAAGCCACTGACAACTTCAATAGTGCAAGAATATTAGGAGAAGGTGGCTTTGGAATTGTTTACAGTGGCAGTTTAGATGATGGAAGGGAAGTGGCTGTGAAGGTTCTTAAACGACATAATCAACATGGTATTCGGGAATTCTTGGCAGAAGTCGAGATGCTGAGCCGTTTACACCATAGAAATCTAGTTAAGTTGATTGGTATCTGTACAGAAGATCAGATTCGCTGCCTGGTATATGAATTGGTTCCTAATGGAAGTGTCGAATCGCATTTACATGGCATTGACAAGTTAACCAGTCCACTTGACTGGGATGCCCGCATGAAGATTGCCCTCGGTGCTGCTCGAGGCCTAGCCTATCTGCATGAAGACTCCAATCCTCGAGTTATTCATCGAGACTTCAAAGCTAGCAACATCTTGTTGGAATATGACTTTACACCAAAAGTTTCAGATTTCGGATTAGCTAGAACTGCACTAGAGGAAGGAAACAAGCACATCTCAACACATGTTATGGGAACTTTTGGTTATCTAGCTCCAGAATATGCAATGACTGGCCACCTTCTTGTAAAGAGCGATGTCTATAGCTATGGTGTTGTCCTTCTTGAGCTACTTACAGGAAGAAAGCCAGTAGACTTGTCGCTTCCACCCGGACAAGAAAATCTCGTTGCTTGGGCTCGTCCACTTCTCACGAGCAAGGAAGGGTTAGATGCAATAACAGACCCAGCTATTAAGCCTGATATCTCCATTGATAGCTTGGCCAGAGTAGCAGCAATTGCTTCTATGTGTGTGCAACCGGAAGTATCTCACAGACCATTTATGGGTGAGGTTGTTCAAGCCCTGAAACTCGTTTGCAATGAGTTCGAAGAAACTAACGATCCAGTTTCAAGAAGTTACAGCCGAGACGAACTTCTAAGTTACATGGACAGTAAATTTGGAGGAATTTCCGGTGAAATACTAAACGCTCCCGAGACCTCGCACACCTTTCCTTCGGGAAAAGAGACAAATGTAGGATTATCGGCATCAGATTTGATTAGTGCATCTGCAAGATTTGAAGGACAGGAACTGGTTTCTTCTAGATGGCATTCATCAAACTCGGAACCATTGAGAACTGGAAGAAAAAAGCACTTATGGCAGAAGGTAAGAAGTTTGTCACGAGGCAGCTTCAGTGAACATGGCTTTTCTGCAAAGTTTTGGCCTGGATTTCGTTAG

Protein sequence

MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAADHAVTPTSHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGHEHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSPGPAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPPLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIGAAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGFR
Homology
BLAST of HG10023477 vs. NCBI nr
Match: XP_038899985.1 (receptor-like serine/threonine-protein kinase ALE2 [Benincasa hispida])

HSP 1 Score: 1664.0 bits (4308), Expect = 0.0e+00
Identity = 838/888 (94.37%), Postives = 851/888 (95.83%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQICFAV SFS CEAA               DHAVTPT
Sbjct: 1   MKTAEISFEIGTLMGFRLLFLLFQICFAVSSFSFCEAAVILPGRLSRSRSVLPDHAVTPT 60

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGHE 120
           SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRA SIALPPT  AKKWGHE
Sbjct: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRATSIALPPTGSAKKWGHE 120

Query: 121 HTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSPG 180
           HTFSPL S HKFRHSRRKFRNN P+PT+RA PPTSSQKGPAAVSPIQS LPSV  GR PG
Sbjct: 121 HTFSPLISFHKFRHSRRKFRNNVPRPTFRALPPTSSQKGPAAVSPIQSPLPSVAGGRLPG 180

Query: 181 PAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTY 240
           PAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTY
Sbjct: 181 PAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTY 240

Query: 241 TPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQQL 300
           TPPGTPCGCVWPIQVKI LDVAVYTFFPLVSELAEEIAD ISLNQSQVRIMGADAASQQL
Sbjct: 241 TPPGTPCGCVWPIQVKITLDVAVYTFFPLVSELAEEIADSISLNQSQVRIMGADAASQQL 300

Query: 301 EKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPPLA 360
           EKTTVLINLVP+GSKFNHNTAFFIYQKFWRRKISIN SLFGRYQVLNVKYPGLPPSPPLA
Sbjct: 301 EKTTVLINLVPRGSKFNHNTAFFIYQKFWRRKISINASLFGRYQVLNVKYPGLPPSPPLA 360

Query: 361 PSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLA 420
           PSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLA
Sbjct: 361 PSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLA 420

Query: 421 WLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIGAAK 480
           WLCLLRCR STHQPAQIP+NLIAS  KPPGTAGL+++GSEPGSSSMPLD DPMTYIGAAK
Sbjct: 421 WLCLLRCRASTHQPAQIPENLIASTRKPPGTAGLLIIGSEPGSSSMPLDTDPMTYIGAAK 480

Query: 481 NFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVE 540
           NFTLKDMEKATDNF++ARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVE
Sbjct: 481 NFTLKDMEKATDNFDTARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVE 540

Query: 541 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 600
           MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG
Sbjct: 541 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 600

Query: 601 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 660
           AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT
Sbjct: 601 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 660

Query: 661 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 720
           FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE
Sbjct: 661 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 720

Query: 721 GLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 780
           GLDAITDPAIK DIS+DSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP
Sbjct: 721 GLDAITDPAIKSDISVDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 780

Query: 781 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQ 840
           VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQ
Sbjct: 781 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQ 840

Query: 841 ELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           ELVSSRWHSSNSEPLRTG+KKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 841 ELVSSRWHSSNSEPLRTGKKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 888

BLAST of HG10023477 vs. NCBI nr
Match: XP_004141683.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucumis sativus] >KGN45527.1 hypothetical protein Csa_016239 [Cucumis sativus])

HSP 1 Score: 1622.4 bits (4200), Expect = 0.0e+00
Identity = 820/888 (92.34%), Postives = 841/888 (94.71%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQI FAV  FS+CEAA               DHAVTPT
Sbjct: 11  MKTAEISFEIGTLMGFRLLFLLFQIGFAVSFFSVCEAAVVLPGRLSGSHSVLRDHAVTPT 70

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGHE 120
           SHGFGRKHHGNL +AAP KPFK+SSPQIHSHYKA QSDDSSRAPSIALPPT PAKKWGHE
Sbjct: 71  SHGFGRKHHGNLYSAAPRKPFKTSSPQIHSHYKAFQSDDSSRAPSIALPPTTPAKKWGHE 130

Query: 121 HTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSPG 180
           HTFSP  S HKFRHSRRKFRNNAPQPTY A PPTSS++GPAAVSPIQS LPS  RGR PG
Sbjct: 131 HTFSPSISFHKFRHSRRKFRNNAPQPTYHALPPTSSRQGPAAVSPIQSPLPSAARGRYPG 190

Query: 181 PAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTY 240
           PAPSPTI+P+HYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTI+CTEPLTY
Sbjct: 191 PAPSPTIRPSHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTISCTEPLTY 250

Query: 241 TPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQQL 300
           TPPGTPCGCVWPIQV+I LDVAVY FFPLVS+LAEEIAD ISLNQSQVRIMGADAASQQL
Sbjct: 251 TPPGTPCGCVWPIQVQITLDVAVYVFFPLVSKLAEEIADSISLNQSQVRIMGADAASQQL 310

Query: 301 EKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPPLA 360
           EKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPSPPLA
Sbjct: 311 EKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINSSLFGRYQVLNVKYPGLPPSPPLA 370

Query: 361 PSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLA 420
           PS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLG NMIAVITISSFTALVMCVGLA
Sbjct: 371 PSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGSNMIAVITISSFTALVMCVGLA 430

Query: 421 WLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIGAAK 480
           WLCLLR RVS H PAQIPQN+IASPTKP GTAGLIMVGSEPGSSSMPLDADPMTYIGAAK
Sbjct: 431 WLCLLRYRVSAHPPAQIPQNMIASPTKPSGTAGLIMVGSEPGSSSMPLDADPMTYIGAAK 490

Query: 481 NFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVE 540
           NFTLKDMEK+TDNF++ARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFLAEVE
Sbjct: 491 NFTLKDMEKSTDNFDTARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFLAEVE 550

Query: 541 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 600
           MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG
Sbjct: 551 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 610

Query: 601 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 660
           AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT
Sbjct: 611 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 670

Query: 661 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 720
           FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE
Sbjct: 671 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 730

Query: 721 GLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 780
           GLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP
Sbjct: 731 GLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 790

Query: 781 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQ 840
           VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTF SGKETNVGLSASDLISASARFEGQ
Sbjct: 791 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFLSGKETNVGLSASDLISASARFEGQ 850

Query: 841 ELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           ELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 851 ELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 898

BLAST of HG10023477 vs. NCBI nr
Match: XP_008462354.1 (PREDICTED: receptor-like serine/threonine-protein kinase ALE2 [Cucumis melo] >ADN34110.1 protein kinase [Cucumis melo subsp. melo])

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 819/891 (91.92%), Postives = 836/891 (93.83%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQICFAV  F +CEAA               DHAVTPT
Sbjct: 11  MKTAEISFEIGTLMGFRLLFLLFQICFAVSFFCVCEAAVVLPGRLSRSHSVLRDHAVTPT 70

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAI-QSDDSSRAPSIALPPTAPAKKWGH 120
           SHGFGRKHHGNLDNAAP KPFK+SSPQIHSHYKAI QSDDSS  PSIALPP  PAKKWGH
Sbjct: 71  SHGFGRKHHGNLDNAAPRKPFKTSSPQIHSHYKAIHQSDDSSLTPSIALPPITPAKKWGH 130

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
           EHTFSP  S HKFRHSRRKFRN+APQPTY A PPTSSQ+GPAAVSPIQS LPS  RGR P
Sbjct: 131 EHTFSPSISFHKFRHSRRKFRNHAPQPTYHALPPTSSQQGPAAVSPIQSPLPSAARGRYP 190

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSY--KKKKSMPPSQVMMLPPPPPNGDCTIACTEP 240
           GP PSPT QPNHYYMPIPAPTTSPMGSY  KKKKSMPPSQVMMLPPPPPNGDCTI+CTEP
Sbjct: 191 GPTPSPTTQPNHYYMPIPAPTTSPMGSYKKKKKKSMPPSQVMMLPPPPPNGDCTISCTEP 250

Query: 241 LTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAAS 300
           LTYTPPGTPCGCVWPIQVKI LDVAVY FFPLVS+LA+EIAD ISLNQSQVRIMGADAAS
Sbjct: 251 LTYTPPGTPCGCVWPIQVKITLDVAVYEFFPLVSKLADEIADSISLNQSQVRIMGADAAS 310

Query: 301 QQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSP 360
           QQLEKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPSP
Sbjct: 311 QQLEKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINASLFGRYQVLNVKYPGLPPSP 370

Query: 361 PLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 420
           PLAPS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV
Sbjct: 371 PLAPSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 430

Query: 421 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 480
           GLAWLCLLR RVS HQPAQIPQNLIASPTKP GTAGLIMVGSEPGSSS  LDADPMTYIG
Sbjct: 431 GLAWLCLLRYRVSAHQPAQIPQNLIASPTKPSGTAGLIMVGSEPGSSSTRLDADPMTYIG 490

Query: 481 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540
           AAKNFTL DMEKATDNF+SARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFLA
Sbjct: 491 AAKNFTLNDMEKATDNFDSARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFLA 550

Query: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 600
           EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI
Sbjct: 551 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 610

Query: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660
           ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV
Sbjct: 611 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 670

Query: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720
           MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT
Sbjct: 671 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 730

Query: 721 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780
           SKEGLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET
Sbjct: 731 SKEGLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 790

Query: 781 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 840
           NDPVSRSYSRDELLSYMDSKFGGISGEILNAPE+S T+ SGKETNVGLSASDLISASARF
Sbjct: 791 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPESSRTYLSGKETNVGLSASDLISASARF 850

Query: 841 EGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           EGQELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 851 EGQELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 901

BLAST of HG10023477 vs. NCBI nr
Match: KAA0059431.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucumis melo var. makuwa] >TYK03894.1 receptor-like serine/threonine-protein kinase ALE2 [Cucumis melo var. makuwa])

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 817/892 (91.59%), Postives = 833/892 (93.39%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGT MGFRLLFLLFQICFAV  FS+CEAA               DHAVTPT
Sbjct: 1   MKTAEISFEIGTPMGFRLLFLLFQICFAVSFFSVCEAAVVLPGRLSRSHSVLRDHAVTPT 60

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAI-QSDDSSRAPSIALPPTAPAKKWGH 120
           SHGFGRKHHGNLDNAAP KPFK+SSPQIHSHYKAI QSDDSS  PSIALPP  PAKKWGH
Sbjct: 61  SHGFGRKHHGNLDNAAPRKPFKTSSPQIHSHYKAIHQSDDSSITPSIALPPITPAKKWGH 120

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
           EHTFSP  S HKFRHSRRKFRN+APQPTY A PPTSSQ+GPAAVSPIQS  PS  RGR P
Sbjct: 121 EHTFSPSISFHKFRHSRRKFRNHAPQPTYHALPPTSSQQGPAAVSPIQSPSPSAARGRYP 180

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSY---KKKKSMPPSQVMMLPPPPPNGDCTIACTE 240
           GP PSPT QPNHYYMPIPAPTTSPMGSY   KKKKSMPPSQVMMLPPPPPNGDCTI+CTE
Sbjct: 181 GPTPSPTTQPNHYYMPIPAPTTSPMGSYKKKKKKKSMPPSQVMMLPPPPPNGDCTISCTE 240

Query: 241 PLTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAA 300
           PLTYTPPGTPCGCVWPIQVKI LDVAVY FFPLVS+LA+EIAD ISLNQSQVRIMGADAA
Sbjct: 241 PLTYTPPGTPCGCVWPIQVKITLDVAVYEFFPLVSKLADEIADSISLNQSQVRIMGADAA 300

Query: 301 SQQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPS 360
           SQQLEKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPS
Sbjct: 301 SQQLEKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINASLFGRYQVLNVKYPGLPPS 360

Query: 361 PPLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC 420
           PPLAPS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC
Sbjct: 361 PPLAPSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC 420

Query: 421 VGLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYI 480
           VGLAWLCLLR RVS HQPAQIPQNLIASPTKP GTAGLIMVGSEPGSSS  LDADPMTYI
Sbjct: 421 VGLAWLCLLRYRVSAHQPAQIPQNLIASPTKPSGTAGLIMVGSEPGSSSTRLDADPMTYI 480

Query: 481 GAAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFL 540
           GAAKNFTL DMEKATDNF+SARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFL
Sbjct: 481 GAAKNFTLNDMEKATDNFDSARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFL 540

Query: 541 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 600
           AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK
Sbjct: 541 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 600

Query: 601 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 660
           IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH
Sbjct: 601 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 660

Query: 661 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 720
           VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL
Sbjct: 661 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 720

Query: 721 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 780
           TSKEGLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE
Sbjct: 721 TSKEGLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 780

Query: 781 TNDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASAR 840
           T DPVSRSYSRDELLSYMDSKFGGISGEILNAPE S T+ SGKETNVGLSASDLISASAR
Sbjct: 781 TKDPVSRSYSRDELLSYMDSKFGGISGEILNAPEPSRTYLSGKETNVGLSASDLISASAR 840

Query: 841 FEGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           FEGQELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 841 FEGQELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 892

BLAST of HG10023477 vs. NCBI nr
Match: XP_022154207.1 (receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Momordica charantia])

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 769/891 (86.31%), Postives = 805/891 (90.35%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEA----------------ADHAVTP 60
           M TAEIS + G  MGFRLLFLLF+I FAV SFS CEA                ADH V+P
Sbjct: 1   MGTAEISSEFGARMGFRLLFLLFEIWFAVCSFSACEAAAVILPSRPSRLRSVLADHGVSP 60

Query: 61  TSHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGH 120
           TSH FG KHHG  DNA PSKPFKSSSPQIHSHY+AIQSDDS RAPSIALPP+APAKKW H
Sbjct: 61  TSHDFGSKHHGKFDNAVPSKPFKSSSPQIHSHYRAIQSDDSRRAPSIALPPSAPAKKWVH 120

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
            H FSP+ S HKF HSRRKFRN+APQPTY +SPP+S ++GPAAVSPIQS L S  RGR  
Sbjct: 121 GHAFSPVISFHKFHHSRRKFRNDAPQPTYHSSPPSSGEQGPAAVSPIQSPLSSAARGRLH 180

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSYKK-KKSMPPSQVMMLPPPPPNGDCTIACTEPL 240
           GPAPSPTIQPNHYYMPIPAPTTSP+GSYKK KKSMPPSQVMMLPPPPPNGDC IACT+PL
Sbjct: 181 GPAPSPTIQPNHYYMPIPAPTTSPLGSYKKRKKSMPPSQVMMLPPPPPNGDCGIACTDPL 240

Query: 241 TYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQ 300
           TYTPPG PC CVWPIQVKI LDVA+YTFFPLVSELAEEIAD I LN SQVRIMGADAASQ
Sbjct: 241 TYTPPGMPCACVWPIQVKISLDVAIYTFFPLVSELAEEIADSIGLNHSQVRIMGADAASQ 300

Query: 301 QLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPP 360
           QLEKTTVLINLVP+G KFNH TAF IYQKFW+RKI IN SLFG YQVLNVKYPGLPPSPP
Sbjct: 301 QLEKTTVLINLVPRGLKFNHTTAFSIYQKFWQRKIFINSSLFGSYQVLNVKYPGLPPSPP 360

Query: 361 LAPSGTSSINDGLNTSNTNAGNAIKPLGVDVP-RRKKEGLGRNMIAVITISSFTALVMCV 420
            APSGTS+INDGLN+SN NAGNAIKPLGVDVP RR+KEGLG NMIAVITISSFTA VMCV
Sbjct: 361 SAPSGTSAINDGLNSSNANAGNAIKPLGVDVPRRRRKEGLGGNMIAVITISSFTAFVMCV 420

Query: 421 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 480
           G+AWLCLLRCRVS HQP Q+PQNLIASPTKPPGTAG IMVG EP SSSMPLDADPMTYIG
Sbjct: 421 GIAWLCLLRCRVSAHQPGQVPQNLIASPTKPPGTAGSIMVGCEPSSSSMPLDADPMTYIG 480

Query: 481 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540
           AAKNFTLKD+E+ATD F++ARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA
Sbjct: 481 AAKNFTLKDIERATDKFDAARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540

Query: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 600
           EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLT PLDWDARMKI
Sbjct: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTGPLDWDARMKI 600

Query: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660
           ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV
Sbjct: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660

Query: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720
           MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT
Sbjct: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720

Query: 721 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780
           SKEGLDAITDPAIK +I IDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET
Sbjct: 721 SKEGLDAITDPAIKSNIPIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780

Query: 781 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 840
           N+P S+SYSRDELLSYMDS+ G +SGEILN  ETS      KETNVGLSASDLISASARF
Sbjct: 781 NEPDSQSYSRDELLSYMDSRLGRMSGEILNTFETSQP----KETNVGLSASDLISASARF 840

Query: 841 EGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           EGQELVSSRWHSSNSEPLRTG+++H WQ++RSLSRGS SEHGFS+KFWPGF
Sbjct: 841 EGQELVSSRWHSSNSEPLRTGKRRHFWQRLRSLSRGSISEHGFSSKFWPGF 887

BLAST of HG10023477 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.3e-168
Identity = 361/726 (49.72%), Postives = 454/726 (62.53%), Query Frame = 0

Query: 163 SPGPAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEP 222
           S  P PSP   PN    P   P     G ++ ++         +   P + DC   C EP
Sbjct: 52  SVAPTPSPAFSPN----PSRIPPLRHKGHHRHRRWHLRRNATAV--SPSSHDCQQTCVEP 111

Query: 223 LTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAAS 282
           LT TP G+PCGCV+P++V+++L VA ++ FP+ +EL  E+A G  L QSQV+IMGA A S
Sbjct: 112 LTSTPFGSPCGCVFPMKVQLLLSVAPFSIFPVTNELEIEVAAGTYLEQSQVKIMGASADS 171

Query: 283 QQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSP 342
           +   KT V INLVP G KF++ TA  IYQ+F  +K+ +N ++FG Y+V ++ YPG+P S 
Sbjct: 172 ENQGKTVVDINLVPLGEKFDNTTATLIYQRFRHKKVPLNETVFGDYEVTHISYPGIPSSS 231

Query: 343 PLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 402
           P          +G  T +   G  I P+       K +G+G   IA+I +S F  +++ V
Sbjct: 232 P----------NGDVTGDAPGGLPI-PINATTFANKSQGIGFRTIAIIALSGFVLILVLV 291

Query: 403 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 462
           G   + +   ++     A  P  L  S  K PG AG +   S   S S  L +   T   
Sbjct: 292 GAISIIVKWKKIGKSSNAVGPA-LAPSINKRPG-AGSMFSSSARSSGSDSLMSSMATCAL 351

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 522
           + K FTL ++EKATD F++ R+LGEGGFG VY GS++DG EVAVK+L R NQ+  REF+A
Sbjct: 352 SVKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIA 411

Query: 523 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 582
           EVEMLSRLHHRNLVKLIGIC E + RCL+YELV NGSVESHLH        LDWDAR+KI
Sbjct: 412 EVEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLH-----EGTLDWDARLKI 471

Query: 583 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 642
           ALGAARGLAYLHEDSNPRVIHRDFKASN+LLE DFTPKVSDFGLAR A  EG++HIST V
Sbjct: 472 ALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREA-TEGSQHISTRV 531

Query: 643 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 702
           MGTFGY+APEYAMTGHLLVKSDVYSYGVVLLELLTGR+PVD+S P G+ENLV WARPLL 
Sbjct: 532 MGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLA 591

Query: 703 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 762
           ++EGL+ + DPA+    + D +A+VAAIASMCV  EVSHRPFMGEVVQALKL+ N+ +ET
Sbjct: 592 NREGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQALKLIYNDADET 651

Query: 763 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 822
                  Y   +  S  DS      G++  AP  S  +        G  AS  I+     
Sbjct: 652 ----CGDYCSQKDSSVPDS--ADFKGDL--APSDSSWWNLTPRLRYG-QASSFITMDYSS 711

Query: 823 EGQELVSSRWHSSN-------------SEPLRTGRKKHLWQKVRSLSRGSFSEHG---FS 873
              E + +R HS++             S PLR  R +  + ++    RGS SEHG    S
Sbjct: 712 GPLEDMENRPHSASSIPRVGGLILPNRSGPLRPMRSRRNFFRL----RGSMSEHGGPSSS 739

BLAST of HG10023477 vs. ExPASy Swiss-Prot
Match: Q6I5Q6 (Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK185 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.2e-88
Identity = 171/338 (50.59%), Postives = 219/338 (64.79%), Query Frame = 0

Query: 442 VGSEPGSSSMPLDADPMTYIG--------AAKNFTLKDMEKATDNFNSARILGEGGFGIV 501
           VGS+   S   LD+    +I         AA  FT +++  AT NF    +LGEGGFG V
Sbjct: 35  VGSDKSKSQGGLDSRKDAFIPRDANGQPIAAHTFTFRELAAATKNFRQDCLLGEGGFGRV 94

Query: 502 YSGSLDDGREVAVKVLKRHNQHGIREFLAEVEMLSRLHHRNLVKLIGICTEDQIRCLVYE 561
           Y G L++G+ VAVK L R+   G REFL EV MLS LHH NLV LIG C +   R LVYE
Sbjct: 95  YKGHLENGQAVAVKQLDRNGLQGNREFLVEVLMLSLLHHDNLVNLIGYCADGDQRLLVYE 154

Query: 562 LVPNGSVESHLHGIDKLTSPLDWDARMKIALGAARGLAYLHEDSNPRVIHRDFKASNILL 621
            +P GS+E HLH I     PLDW+ RMKIA GAA+GL +LH+ +NP VI+RDFK+SNILL
Sbjct: 155 FMPLGSLEDHLHDIPPDKEPLDWNTRMKIAAGAAKGLEFLHDKANPPVIYRDFKSSNILL 214

Query: 622 EYDFTPKVSDFGLARTALEEGNKHISTHVMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLL 681
              + PK+SDFGLA+        H+ST VMGT+GY APEYAMTG L VKSDVYS+GVV L
Sbjct: 215 GEGYHPKLSDFGLAKLGPVGDKTHVSTRVMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFL 274

Query: 682 ELLTGRKPVDLSLPPGQENLVAWARPLLTSKEGLDAITDPAIKPDISIDSLARVAAIASM 741
           EL+TGRK +D + P G++NLVAWARPL   +     + DP +     +  L +  A+A+M
Sbjct: 275 ELITGRKAIDNTKPLGEQNLVAWARPLFKDRRKFPKMADPLLAGRFPMRGLYQALAVAAM 334

Query: 742 CVQPEVSHRPFMGEVVQALKLVCNEFEETNDPVSRSYS 772
           C+Q + + RPF+G+VV AL  + ++  + N PV  S S
Sbjct: 335 CLQEQAATRPFIGDVVTALSYLASQTYDPNTPVQHSRS 372

BLAST of HG10023477 vs. ExPASy Swiss-Prot
Match: Q9FE20 (Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 6.6e-85
Identity = 186/391 (47.57%), Postives = 238/391 (60.87%), Query Frame = 0

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDD-GREVAVKVLKRHNQHGIREFL 522
           AA  F  +++  AT NF+    LGEGGFG VY G LD  G+ VAVK L R+   G REFL
Sbjct: 70  AAHTFAFRELAAATMNFHPDTFLGEGGFGRVYKGRLDSTGQVVAVKQLDRNGLQGNREFL 129

Query: 523 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 582
            EV MLS LHH NLV LIG C +   R LVYE +P GS+E HLH +      LDW+ RMK
Sbjct: 130 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMK 189

Query: 583 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 642
           IA GAA+GL +LH+ +NP VI+RDFK+SNILL+  F PK+SDFGLA+        H+ST 
Sbjct: 190 IAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTR 249

Query: 643 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 702
           VMGT+GY APEYAMTG L VKSDVYS+GVV LEL+TGRK +D  +P G++NLVAWARPL 
Sbjct: 250 VMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDSEMPHGEQNLVAWARPLF 309

Query: 703 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 762
             +     + DP +K      +L +  A+ASMC+Q + + RP + +VV AL  + N+  +
Sbjct: 310 NDRRKFIKLADPRLKGRFPTRALYQALAVASMCIQEQAATRPLIADVVTALSYLANQAYD 369

Query: 763 TNDPVSRSYSRDE----LLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSAS---D 822
            +   SR  +RDE    L++  D   GG SG   +  E S    S +ET   L+     +
Sbjct: 370 PSKDDSRR-NRDERGARLITRNDD--GGGSGSKFDL-EGSEKEDSPRETARILNRDINRE 429

Query: 823 LISASARFEGQELVSSRWHS--SNSEPLRTG 844
              A A+  G+ L   R  S    SE   TG
Sbjct: 430 RAVAEAKMWGESLREKRRQSEQGTSESNSTG 456

BLAST of HG10023477 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.6e-83
Identity = 165/336 (49.11%), Postives = 215/336 (63.99%), Query Frame = 0

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLD-DGREVAVKVLKRHNQHGIREFL 522
           AA+ FT +++  AT NF    +LGEGGFG VY G L+  G+ VAVK L R+   G REFL
Sbjct: 67  AAQTFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFL 126

Query: 523 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 582
            EV MLS LHH NLV LIG C +   R LVYE +P GS+E HLH +     PLDW  RM 
Sbjct: 127 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMT 186

Query: 583 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 642
           IA GAA+GL YLH+ +NP VI+RD K+SNILL   + PK+SDFGLA+        H+ST 
Sbjct: 187 IAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTR 246

Query: 643 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 702
           VMGT+GY APEYAMTG L +KSDVYS+GVV LEL+TGRK +D +  PG+ NLVAWARPL 
Sbjct: 247 VMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLF 306

Query: 703 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 762
             +     + DP+++    +  L +  A+A+MC+Q + + RP +G+VV AL  + ++  +
Sbjct: 307 KDRRKFPKMADPSLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFD 366

Query: 763 TNDPV---SRSYSRDELLSYMDSKFGGISGEILNAP 795
            N P    SRS S    +   D +     G  L++P
Sbjct: 367 PNAPSGQNSRSGSGPPFIRTRDDRRSLGDGSSLDSP 402

BLAST of HG10023477 vs. ExPASy Swiss-Prot
Match: Q9LDZ5 (Probable serine/threonine-protein kinase PBL21 OS=Arabidopsis thaliana OX=3702 GN=PBL21 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 6.2e-83
Identity = 157/320 (49.06%), Postives = 209/320 (65.31%), Query Frame = 0

Query: 462 GAAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFL 521
           G A++FT K++  AT NF    +LGEGGFG VY G LD G+ VA+K L      G REF+
Sbjct: 61  GGARSFTFKELAAATRNFREVNLLGEGGFGRVYKGRLDSGQVVAIKQLNPDGLQGNREFI 120

Query: 522 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 581
            EV MLS LHH NLV LIG CT    R LVYE +P GS+E HL  ++    PL W+ RMK
Sbjct: 121 VEVLMLSLLHHPNLVTLIGYCTSGDQRLLVYEYMPMGSLEDHLFDLESNQEPLSWNTRMK 180

Query: 582 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 641
           IA+GAARG+ YLH  +NP VI+RD K++NILL+ +F+PK+SDFGLA+        H+ST 
Sbjct: 181 IAVGAARGIEYLHCTANPPVIYRDLKSANILLDKEFSPKLSDFGLAKLGPVGDRTHVSTR 240

Query: 642 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 701
           VMGT+GY APEYAM+G L VKSD+Y +GVVLLEL+TGRK +DL    G++NLV W+RP L
Sbjct: 241 VMGTYGYCAPEYAMSGKLTVKSDIYCFGVVLLELITGRKAIDLGQKQGEQNLVTWSRPYL 300

Query: 702 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVC----- 761
             ++    + DP+++       L    AI +MC+  E  +RPF+G++V AL+ +      
Sbjct: 301 KDQKKFGHLVDPSLRGKYPRRCLNYAIAIIAMCLNEEAHYRPFIGDIVVALEYLAAQSRS 360

Query: 762 NEFEETNDP---VSRSYSRD 774
           +E    + P   +SR+  RD
Sbjct: 361 HEARNVSSPSPEISRTPRRD 380

BLAST of HG10023477 vs. ExPASy TrEMBL
Match: A0A0A0KAZ5 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G451330 PE=4 SV=1)

HSP 1 Score: 1622.4 bits (4200), Expect = 0.0e+00
Identity = 820/888 (92.34%), Postives = 841/888 (94.71%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQI FAV  FS+CEAA               DHAVTPT
Sbjct: 11  MKTAEISFEIGTLMGFRLLFLLFQIGFAVSFFSVCEAAVVLPGRLSGSHSVLRDHAVTPT 70

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGHE 120
           SHGFGRKHHGNL +AAP KPFK+SSPQIHSHYKA QSDDSSRAPSIALPPT PAKKWGHE
Sbjct: 71  SHGFGRKHHGNLYSAAPRKPFKTSSPQIHSHYKAFQSDDSSRAPSIALPPTTPAKKWGHE 130

Query: 121 HTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSPG 180
           HTFSP  S HKFRHSRRKFRNNAPQPTY A PPTSS++GPAAVSPIQS LPS  RGR PG
Sbjct: 131 HTFSPSISFHKFRHSRRKFRNNAPQPTYHALPPTSSRQGPAAVSPIQSPLPSAARGRYPG 190

Query: 181 PAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEPLTY 240
           PAPSPTI+P+HYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTI+CTEPLTY
Sbjct: 191 PAPSPTIRPSHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTISCTEPLTY 250

Query: 241 TPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQQL 300
           TPPGTPCGCVWPIQV+I LDVAVY FFPLVS+LAEEIAD ISLNQSQVRIMGADAASQQL
Sbjct: 251 TPPGTPCGCVWPIQVQITLDVAVYVFFPLVSKLAEEIADSISLNQSQVRIMGADAASQQL 310

Query: 301 EKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPPLA 360
           EKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPSPPLA
Sbjct: 311 EKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINSSLFGRYQVLNVKYPGLPPSPPLA 370

Query: 361 PSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCVGLA 420
           PS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLG NMIAVITISSFTALVMCVGLA
Sbjct: 371 PSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGSNMIAVITISSFTALVMCVGLA 430

Query: 421 WLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIGAAK 480
           WLCLLR RVS H PAQIPQN+IASPTKP GTAGLIMVGSEPGSSSMPLDADPMTYIGAAK
Sbjct: 431 WLCLLRYRVSAHPPAQIPQNMIASPTKPSGTAGLIMVGSEPGSSSMPLDADPMTYIGAAK 490

Query: 481 NFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVE 540
           NFTLKDMEK+TDNF++ARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFLAEVE
Sbjct: 491 NFTLKDMEKSTDNFDTARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFLAEVE 550

Query: 541 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 600
           MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG
Sbjct: 551 MLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALG 610

Query: 601 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 660
           AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT
Sbjct: 611 AARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHVMGT 670

Query: 661 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 720
           FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE
Sbjct: 671 FGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKE 730

Query: 721 GLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 780
           GLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP
Sbjct: 731 GLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETNDP 790

Query: 781 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQ 840
           VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTF SGKETNVGLSASDLISASARFEGQ
Sbjct: 791 VSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFLSGKETNVGLSASDLISASARFEGQ 850

Query: 841 ELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           ELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 851 ELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 898

BLAST of HG10023477 vs. ExPASy TrEMBL
Match: E5GCB1 (Protein kinase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 819/891 (91.92%), Postives = 836/891 (93.83%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQICFAV  F +CEAA               DHAVTPT
Sbjct: 11  MKTAEISFEIGTLMGFRLLFLLFQICFAVSFFCVCEAAVVLPGRLSRSHSVLRDHAVTPT 70

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAI-QSDDSSRAPSIALPPTAPAKKWGH 120
           SHGFGRKHHGNLDNAAP KPFK+SSPQIHSHYKAI QSDDSS  PSIALPP  PAKKWGH
Sbjct: 71  SHGFGRKHHGNLDNAAPRKPFKTSSPQIHSHYKAIHQSDDSSLTPSIALPPITPAKKWGH 130

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
           EHTFSP  S HKFRHSRRKFRN+APQPTY A PPTSSQ+GPAAVSPIQS LPS  RGR P
Sbjct: 131 EHTFSPSISFHKFRHSRRKFRNHAPQPTYHALPPTSSQQGPAAVSPIQSPLPSAARGRYP 190

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSY--KKKKSMPPSQVMMLPPPPPNGDCTIACTEP 240
           GP PSPT QPNHYYMPIPAPTTSPMGSY  KKKKSMPPSQVMMLPPPPPNGDCTI+CTEP
Sbjct: 191 GPTPSPTTQPNHYYMPIPAPTTSPMGSYKKKKKKSMPPSQVMMLPPPPPNGDCTISCTEP 250

Query: 241 LTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAAS 300
           LTYTPPGTPCGCVWPIQVKI LDVAVY FFPLVS+LA+EIAD ISLNQSQVRIMGADAAS
Sbjct: 251 LTYTPPGTPCGCVWPIQVKITLDVAVYEFFPLVSKLADEIADSISLNQSQVRIMGADAAS 310

Query: 301 QQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSP 360
           QQLEKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPSP
Sbjct: 311 QQLEKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINASLFGRYQVLNVKYPGLPPSP 370

Query: 361 PLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 420
           PLAPS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV
Sbjct: 371 PLAPSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 430

Query: 421 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 480
           GLAWLCLLR RVS HQPAQIPQNLIASPTKP GTAGLIMVGSEPGSSS  LDADPMTYIG
Sbjct: 431 GLAWLCLLRYRVSAHQPAQIPQNLIASPTKPSGTAGLIMVGSEPGSSSTRLDADPMTYIG 490

Query: 481 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540
           AAKNFTL DMEKATDNF+SARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFLA
Sbjct: 491 AAKNFTLNDMEKATDNFDSARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFLA 550

Query: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 600
           EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI
Sbjct: 551 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 610

Query: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660
           ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV
Sbjct: 611 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 670

Query: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720
           MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT
Sbjct: 671 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 730

Query: 721 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780
           SKEGLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET
Sbjct: 731 SKEGLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 790

Query: 781 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 840
           NDPVSRSYSRDELLSYMDSKFGGISGEILNAPE+S T+ SGKETNVGLSASDLISASARF
Sbjct: 791 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPESSRTYLSGKETNVGLSASDLISASARF 850

Query: 841 EGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           EGQELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 851 EGQELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 901

BLAST of HG10023477 vs. ExPASy TrEMBL
Match: A0A1S3CGS9 (receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo OX=3656 GN=LOC103500727 PE=4 SV=1)

HSP 1 Score: 1609.7 bits (4167), Expect = 0.0e+00
Identity = 819/891 (91.92%), Postives = 836/891 (93.83%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGTLMGFRLLFLLFQICFAV  F +CEAA               DHAVTPT
Sbjct: 11  MKTAEISFEIGTLMGFRLLFLLFQICFAVSFFCVCEAAVVLPGRLSRSHSVLRDHAVTPT 70

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAI-QSDDSSRAPSIALPPTAPAKKWGH 120
           SHGFGRKHHGNLDNAAP KPFK+SSPQIHSHYKAI QSDDSS  PSIALPP  PAKKWGH
Sbjct: 71  SHGFGRKHHGNLDNAAPRKPFKTSSPQIHSHYKAIHQSDDSSLTPSIALPPITPAKKWGH 130

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
           EHTFSP  S HKFRHSRRKFRN+APQPTY A PPTSSQ+GPAAVSPIQS LPS  RGR P
Sbjct: 131 EHTFSPSISFHKFRHSRRKFRNHAPQPTYHALPPTSSQQGPAAVSPIQSPLPSAARGRYP 190

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSY--KKKKSMPPSQVMMLPPPPPNGDCTIACTEP 240
           GP PSPT QPNHYYMPIPAPTTSPMGSY  KKKKSMPPSQVMMLPPPPPNGDCTI+CTEP
Sbjct: 191 GPTPSPTTQPNHYYMPIPAPTTSPMGSYKKKKKKSMPPSQVMMLPPPPPNGDCTISCTEP 250

Query: 241 LTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAAS 300
           LTYTPPGTPCGCVWPIQVKI LDVAVY FFPLVS+LA+EIAD ISLNQSQVRIMGADAAS
Sbjct: 251 LTYTPPGTPCGCVWPIQVKITLDVAVYEFFPLVSKLADEIADSISLNQSQVRIMGADAAS 310

Query: 301 QQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSP 360
           QQLEKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPSP
Sbjct: 311 QQLEKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINASLFGRYQVLNVKYPGLPPSP 370

Query: 361 PLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 420
           PLAPS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV
Sbjct: 371 PLAPSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 430

Query: 421 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 480
           GLAWLCLLR RVS HQPAQIPQNLIASPTKP GTAGLIMVGSEPGSSS  LDADPMTYIG
Sbjct: 431 GLAWLCLLRYRVSAHQPAQIPQNLIASPTKPSGTAGLIMVGSEPGSSSTRLDADPMTYIG 490

Query: 481 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540
           AAKNFTL DMEKATDNF+SARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFLA
Sbjct: 491 AAKNFTLNDMEKATDNFDSARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFLA 550

Query: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 600
           EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI
Sbjct: 551 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 610

Query: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660
           ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV
Sbjct: 611 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 670

Query: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720
           MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT
Sbjct: 671 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 730

Query: 721 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780
           SKEGLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET
Sbjct: 731 SKEGLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 790

Query: 781 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 840
           NDPVSRSYSRDELLSYMDSKFGGISGEILNAPE+S T+ SGKETNVGLSASDLISASARF
Sbjct: 791 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPESSRTYLSGKETNVGLSASDLISASARF 850

Query: 841 EGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           EGQELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 851 EGQELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 901

BLAST of HG10023477 vs. ExPASy TrEMBL
Match: A0A5A7V135 (Receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00890 PE=4 SV=1)

HSP 1 Score: 1602.8 bits (4149), Expect = 0.0e+00
Identity = 817/892 (91.59%), Postives = 833/892 (93.39%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEAA---------------DHAVTPT 60
           MKTAEISF+IGT MGFRLLFLLFQICFAV  FS+CEAA               DHAVTPT
Sbjct: 1   MKTAEISFEIGTPMGFRLLFLLFQICFAVSFFSVCEAAVVLPGRLSRSHSVLRDHAVTPT 60

Query: 61  SHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAI-QSDDSSRAPSIALPPTAPAKKWGH 120
           SHGFGRKHHGNLDNAAP KPFK+SSPQIHSHYKAI QSDDSS  PSIALPP  PAKKWGH
Sbjct: 61  SHGFGRKHHGNLDNAAPRKPFKTSSPQIHSHYKAIHQSDDSSITPSIALPPITPAKKWGH 120

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
           EHTFSP  S HKFRHSRRKFRN+APQPTY A PPTSSQ+GPAAVSPIQS  PS  RGR P
Sbjct: 121 EHTFSPSISFHKFRHSRRKFRNHAPQPTYHALPPTSSQQGPAAVSPIQSPSPSAARGRYP 180

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSY---KKKKSMPPSQVMMLPPPPPNGDCTIACTE 240
           GP PSPT QPNHYYMPIPAPTTSPMGSY   KKKKSMPPSQVMMLPPPPPNGDCTI+CTE
Sbjct: 181 GPTPSPTTQPNHYYMPIPAPTTSPMGSYKKKKKKKSMPPSQVMMLPPPPPNGDCTISCTE 240

Query: 241 PLTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAA 300
           PLTYTPPGTPCGCVWPIQVKI LDVAVY FFPLVS+LA+EIAD ISLNQSQVRIMGADAA
Sbjct: 241 PLTYTPPGTPCGCVWPIQVKITLDVAVYEFFPLVSKLADEIADSISLNQSQVRIMGADAA 300

Query: 301 SQQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPS 360
           SQQLEKTTV+INLVP+GS+FNHNTAF IYQKFW RKISIN SLFGRYQVLNVKYPGLPPS
Sbjct: 301 SQQLEKTTVIINLVPRGSRFNHNTAFSIYQKFWGRKISINASLFGRYQVLNVKYPGLPPS 360

Query: 361 PPLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC 420
           PPLAPS TSSINDGLNTSNTNAG AIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC
Sbjct: 361 PPLAPSSTSSINDGLNTSNTNAGTAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMC 420

Query: 421 VGLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYI 480
           VGLAWLCLLR RVS HQPAQIPQNLIASPTKP GTAGLIMVGSEPGSSS  LDADPMTYI
Sbjct: 421 VGLAWLCLLRYRVSAHQPAQIPQNLIASPTKPSGTAGLIMVGSEPGSSSTRLDADPMTYI 480

Query: 481 GAAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFL 540
           GAAKNFTL DMEKATDNF+SARILGEGGFGIVYSGSL+DGR+VAVKVLKRHNQHGIREFL
Sbjct: 481 GAAKNFTLNDMEKATDNFDSARILGEGGFGIVYSGSLEDGRDVAVKVLKRHNQHGIREFL 540

Query: 541 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 600
           AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK
Sbjct: 541 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 600

Query: 601 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 660
           IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH
Sbjct: 601 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 660

Query: 661 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 720
           VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL
Sbjct: 661 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 720

Query: 721 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 780
           TSKEGLDAITDPAIK DISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE
Sbjct: 721 TSKEGLDAITDPAIKSDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 780

Query: 781 TNDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASAR 840
           T DPVSRSYSRDELLSYMDSKFGGISGEILNAPE S T+ SGKETNVGLSASDLISASAR
Sbjct: 781 TKDPVSRSYSRDELLSYMDSKFGGISGEILNAPEPSRTYLSGKETNVGLSASDLISASAR 840

Query: 841 FEGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           FEGQELVSSRWHSSNSEPLRTGRKKHLWQK+RSLSRGSFSEHGFSAK WPGF
Sbjct: 841 FEGQELVSSRWHSSNSEPLRTGRKKHLWQKLRSLSRGSFSEHGFSAKLWPGF 892

BLAST of HG10023477 vs. ExPASy TrEMBL
Match: A0A6J1DL27 (receptor-like serine/threonine-protein kinase ALE2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021513 PE=4 SV=1)

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 769/891 (86.31%), Postives = 805/891 (90.35%), Query Frame = 0

Query: 1   MKTAEISFQIGTLMGFRLLFLLFQICFAVRSFSICEA----------------ADHAVTP 60
           M TAEIS + G  MGFRLLFLLF+I FAV SFS CEA                ADH V+P
Sbjct: 1   MGTAEISSEFGARMGFRLLFLLFEIWFAVCSFSACEAAAVILPSRPSRLRSVLADHGVSP 60

Query: 61  TSHGFGRKHHGNLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSRAPSIALPPTAPAKKWGH 120
           TSH FG KHHG  DNA PSKPFKSSSPQIHSHY+AIQSDDS RAPSIALPP+APAKKW H
Sbjct: 61  TSHDFGSKHHGKFDNAVPSKPFKSSSPQIHSHYRAIQSDDSRRAPSIALPPSAPAKKWVH 120

Query: 121 EHTFSPLTSSHKFRHSRRKFRNNAPQPTYRASPPTSSQKGPAAVSPIQSLLPSVVRGRSP 180
            H FSP+ S HKF HSRRKFRN+APQPTY +SPP+S ++GPAAVSPIQS L S  RGR  
Sbjct: 121 GHAFSPVISFHKFHHSRRKFRNDAPQPTYHSSPPSSGEQGPAAVSPIQSPLSSAARGRLH 180

Query: 181 GPAPSPTIQPNHYYMPIPAPTTSPMGSYKK-KKSMPPSQVMMLPPPPPNGDCTIACTEPL 240
           GPAPSPTIQPNHYYMPIPAPTTSP+GSYKK KKSMPPSQVMMLPPPPPNGDC IACT+PL
Sbjct: 181 GPAPSPTIQPNHYYMPIPAPTTSPLGSYKKRKKSMPPSQVMMLPPPPPNGDCGIACTDPL 240

Query: 241 TYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQ 300
           TYTPPG PC CVWPIQVKI LDVA+YTFFPLVSELAEEIAD I LN SQVRIMGADAASQ
Sbjct: 241 TYTPPGMPCACVWPIQVKISLDVAIYTFFPLVSELAEEIADSIGLNHSQVRIMGADAASQ 300

Query: 301 QLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPP 360
           QLEKTTVLINLVP+G KFNH TAF IYQKFW+RKI IN SLFG YQVLNVKYPGLPPSPP
Sbjct: 301 QLEKTTVLINLVPRGLKFNHTTAFSIYQKFWQRKIFINSSLFGSYQVLNVKYPGLPPSPP 360

Query: 361 LAPSGTSSINDGLNTSNTNAGNAIKPLGVDVP-RRKKEGLGRNMIAVITISSFTALVMCV 420
            APSGTS+INDGLN+SN NAGNAIKPLGVDVP RR+KEGLG NMIAVITISSFTA VMCV
Sbjct: 361 SAPSGTSAINDGLNSSNANAGNAIKPLGVDVPRRRRKEGLGGNMIAVITISSFTAFVMCV 420

Query: 421 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 480
           G+AWLCLLRCRVS HQP Q+PQNLIASPTKPPGTAG IMVG EP SSSMPLDADPMTYIG
Sbjct: 421 GIAWLCLLRCRVSAHQPGQVPQNLIASPTKPPGTAGSIMVGCEPSSSSMPLDADPMTYIG 480

Query: 481 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540
           AAKNFTLKD+E+ATD F++ARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA
Sbjct: 481 AAKNFTLKDIERATDKFDAARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 540

Query: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 600
           EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLT PLDWDARMKI
Sbjct: 541 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTGPLDWDARMKI 600

Query: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660
           ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV
Sbjct: 601 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 660

Query: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720
           MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT
Sbjct: 661 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 720

Query: 721 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780
           SKEGLDAITDPAIK +I IDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET
Sbjct: 721 SKEGLDAITDPAIKSNIPIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 780

Query: 781 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 840
           N+P S+SYSRDELLSYMDS+ G +SGEILN  ETS      KETNVGLSASDLISASARF
Sbjct: 781 NEPDSQSYSRDELLSYMDSRLGRMSGEILNTFETSQP----KETNVGLSASDLISASARF 840

Query: 841 EGQELVSSRWHSSNSEPLRTGRKKHLWQKVRSLSRGSFSEHGFSAKFWPGF 874
           EGQELVSSRWHSSNSEPLRTG+++H WQ++RSLSRGS SEHGFS+KFWPGF
Sbjct: 841 EGQELVSSRWHSSNSEPLRTGKRRHFWQRLRSLSRGSISEHGFSSKFWPGF 887

BLAST of HG10023477 vs. TAIR 10
Match: AT5G56890.1 (Protein kinase superfamily protein )

HSP 1 Score: 754.6 bits (1947), Expect = 8.9e-218
Identity = 451/871 (51.78%), Postives = 557/871 (63.95%), Query Frame = 0

Query: 39   DHAVTPTSH------GFGRKHHG-----NLDNAAPSKPFKSSSPQIHSHYKAIQSDDSSR 98
            DHA  P  H      G   K  G           P  P K+S P I              
Sbjct: 257  DHAANPVKHPPPSDNGDDSKSPGAAPANETAKPLPVFPHKASPPSIAPSAPKFNRHSHHT 316

Query: 99   APSIALPP-TAPAKKWGHEHTFS--PLTSSHKFRHSRRKFRNN---APQPTYRASPPTSS 158
            +PS   PP + P+    H  + S  PL+S H+    R+K  ++   +P P +  SP  S+
Sbjct: 317  SPSTTPPPDSTPSNVHHHPSSPSPPPLSSHHQHHQERKKIADSPAPSPLPPHLISPKKSN 376

Query: 159  QKGPAAVSPIQSLLPSVVRGRSPGPAPSPTIQPNHYYMPI------PAPTTSPMGSYKKK 218
            +KG     P     PS        P P   I P H  +        PA   SP   +  +
Sbjct: 377  RKGSMTPPPQSHHAPS-------PPIPDSLISPAHAPVSFSMKRISPALAPSPTQVFPLR 436

Query: 219  KSMPPSQ---------VMMLPPPPPNGDC-TIACTEPLTYTPPGTPCGCVWPIQVKIILD 278
             S  PS+         +   PPPPPN DC +  C EP T TPPG+PCGCVWPIQV++ L 
Sbjct: 437  SSSRPSKSRKFPLGPPLQAFPPPPPNSDCSSTICLEPYTNTPPGSPCGCVWPIQVELRLS 496

Query: 279  VAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAASQQLEKTTVLINLVPKGSKFNHNT 338
            +A+Y FFP+VSE A EI+ G+ + QSQVRIMGA+AAS+Q EK+ VLI+LVP G KF++ T
Sbjct: 497  MALYDFFPMVSEFAREISAGVFMKQSQVRIMGANAASEQPEKSIVLIDLVPLGDKFDNMT 556

Query: 339  AFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSPPLAPSGTSSINDGLNTSNTNAGN 398
            A   YQ+FW +K+ I+  +FG Y V+ V+YPGLP SPP   SG + I+ G  + N N G 
Sbjct: 557  AMLTYQRFWSKKVYIDEPIFGGYDVIYVRYPGLPASPP--TSGMTIIDQGPYSGNNN-GR 616

Query: 399  AIKPLGVDVPRR-KKEGLGRNMIAVITISSFTALVMCVGLAWLCLL-----RCRVSTHQP 458
            A+KPLGVDVPR+ +K+ L    IAVI +S+   + +C  + W  +      R R+S   P
Sbjct: 617  AVKPLGVDVPRKPRKKELNGGSIAVIVLSAAAFIGLCFVIVWFLVFRRQRDRRRLSKRTP 676

Query: 459  AQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIGAAKNFTLKDMEKATDNF 518
               P   + S +KP G+A   + GS   S+S+  ++    +  +AK FT  ++ KAT+NF
Sbjct: 677  LARPS--LPSLSKPSGSA-RSLTGSRFSSTSLSFESSIAPFTLSAKTFTASEIMKATNNF 736

Query: 519  NSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLAEVEMLSRLHHRNLVKLI 578
            + +R+LGEGGFG VY G  DDG +VAVKVLKR +Q G REFLAEVEMLSRLHHRNLV LI
Sbjct: 737  DESRVLGEGGFGRVYEGVFDDGTKVAVKVLKRDDQQGSREFLAEVEMLSRLHHRNLVNLI 796

Query: 579  GICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKIALGAARGLAYLHEDSNP 638
            GIC ED+ R LVYEL+PNGSVESHLHGIDK +SPLDWDAR+KIALGAARGLAYLHEDS+P
Sbjct: 797  GICIEDRNRSLVYELIPNGSVESHLHGIDKASSPLDWDARLKIALGAARGLAYLHEDSSP 856

Query: 639  RVIHRDFKASNILLEYDFTPKVSDFGLARTAL-EEGNKHISTHVMGTFGYLAPEYAMTGH 698
            RVIHRDFK+SNILLE DFTPKVSDFGLAR AL +E N+HIST VMGTFGY+APEYAMTGH
Sbjct: 857  RVIHRDFKSSNILLENDFTPKVSDFGLARNALDDEDNRHISTRVMGTFGYVAPEYAMTGH 916

Query: 699  LLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLTSKEGLDAITDPAIKPD 758
            LLVKSDVYSYGVVLLELLTGRKPVD+S PPGQENLV+W RP LTS EGL AI D ++ P+
Sbjct: 917  LLVKSDVYSYGVVLLELLTGRKPVDMSQPPGQENLVSWTRPFLTSAEGLAAIIDQSLGPE 976

Query: 759  ISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEETND-----PVSRSYSRD 818
            IS DS+A+VAAIASMCVQPEVSHRPFMGEVVQALKLV NE +E  +      +S+   RD
Sbjct: 977  ISFDSIAKVAAIASMCVQPEVSHRPFMGEVVQALKLVSNECDEAKELNSLTSISKDDFRD 1036

Query: 819  ELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARFEGQELVSSRWH 865
            +  +  +S  G  S  +   P   + + S  +T  GLS S++ + S RFE Q        
Sbjct: 1037 D--TQAESSCGDSSARMARYPLLPN-YDSEPDTERGLSTSEMYTGSGRFERQ-------- 1096

BLAST of HG10023477 vs. TAIR 10
Match: AT2G20300.1 (Protein kinase superfamily protein )

HSP 1 Score: 595.1 bits (1533), Expect = 9.0e-170
Identity = 361/726 (49.72%), Postives = 454/726 (62.53%), Query Frame = 0

Query: 163 SPGPAPSPTIQPNHYYMPIPAPTTSPMGSYKKKKSMPPSQVMMLPPPPPNGDCTIACTEP 222
           S  P PSP   PN    P   P     G ++ ++         +   P + DC   C EP
Sbjct: 52  SVAPTPSPAFSPN----PSRIPPLRHKGHHRHRRWHLRRNATAV--SPSSHDCQQTCVEP 111

Query: 223 LTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADGISLNQSQVRIMGADAAS 282
           LT TP G+PCGCV+P++V+++L VA ++ FP+ +EL  E+A G  L QSQV+IMGA A S
Sbjct: 112 LTSTPFGSPCGCVFPMKVQLLLSVAPFSIFPVTNELEIEVAAGTYLEQSQVKIMGASADS 171

Query: 283 QQLEKTTVLINLVPKGSKFNHNTAFFIYQKFWRRKISINPSLFGRYQVLNVKYPGLPPSP 342
           +   KT V INLVP G KF++ TA  IYQ+F  +K+ +N ++FG Y+V ++ YPG+P S 
Sbjct: 172 ENQGKTVVDINLVPLGEKFDNTTATLIYQRFRHKKVPLNETVFGDYEVTHISYPGIPSSS 231

Query: 343 PLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGRNMIAVITISSFTALVMCV 402
           P          +G  T +   G  I P+       K +G+G   IA+I +S F  +++ V
Sbjct: 232 P----------NGDVTGDAPGGLPI-PINATTFANKSQGIGFRTIAIIALSGFVLILVLV 291

Query: 403 GLAWLCLLRCRVSTHQPAQIPQNLIASPTKPPGTAGLIMVGSEPGSSSMPLDADPMTYIG 462
           G   + +   ++     A  P  L  S  K PG AG +   S   S S  L +   T   
Sbjct: 292 GAISIIVKWKKIGKSSNAVGPA-LAPSINKRPG-AGSMFSSSARSSGSDSLMSSMATCAL 351

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDDGREVAVKVLKRHNQHGIREFLA 522
           + K FTL ++EKATD F++ R+LGEGGFG VY GS++DG EVAVK+L R NQ+  REF+A
Sbjct: 352 SVKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIA 411

Query: 523 EVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMKI 582
           EVEMLSRLHHRNLVKLIGIC E + RCL+YELV NGSVESHLH        LDWDAR+KI
Sbjct: 412 EVEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLH-----EGTLDWDARLKI 471

Query: 583 ALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTHV 642
           ALGAARGLAYLHEDSNPRVIHRDFKASN+LLE DFTPKVSDFGLAR A  EG++HIST V
Sbjct: 472 ALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREA-TEGSQHISTRV 531

Query: 643 MGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLLT 702
           MGTFGY+APEYAMTGHLLVKSDVYSYGVVLLELLTGR+PVD+S P G+ENLV WARPLL 
Sbjct: 532 MGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLA 591

Query: 703 SKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEET 762
           ++EGL+ + DPA+    + D +A+VAAIASMCV  EVSHRPFMGEVVQALKL+ N+ +ET
Sbjct: 592 NREGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQALKLIYNDADET 651

Query: 763 NDPVSRSYSRDELLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSASDLISASARF 822
                  Y   +  S  DS      G++  AP  S  +        G  AS  I+     
Sbjct: 652 ----CGDYCSQKDSSVPDS--ADFKGDL--APSDSSWWNLTPRLRYG-QASSFITMDYSS 711

Query: 823 EGQELVSSRWHSSN-------------SEPLRTGRKKHLWQKVRSLSRGSFSEHG---FS 873
              E + +R HS++             S PLR  R +  + ++    RGS SEHG    S
Sbjct: 712 GPLEDMENRPHSASSIPRVGGLILPNRSGPLRPMRSRRNFFRL----RGSMSEHGGPSSS 739

BLAST of HG10023477 vs. TAIR 10
Match: AT4G02010.1 (Protein kinase superfamily protein )

HSP 1 Score: 383.3 bits (983), Expect = 5.4e-106
Identity = 245/568 (43.13%), Postives = 324/568 (57.04%), Query Frame = 0

Query: 206 LPPPPPNGDCTIACTEPLTYTPPGTPCGCVWPIQVKIILDVAVYTFFPLVSELAEEIADG 265
           L  PP +   +  C   +        C CV+PI++ I+L     T  P  +    E A  
Sbjct: 139 LTQPPLSPSISNCCKSDMVLKRRSIGCHCVYPIKLDILLLNVSET--PSWNMFLNEFATQ 198

Query: 266 ISLNQSQVRIMGADAASQQLEKTTVLINLVP-KGSKFNHNTAFFIYQKFWRRKISINPSL 325
           + L   Q+ ++     S  L +  + +++ P  G  F+ + A  I       KI  +P+L
Sbjct: 199 LGLLPHQIELINFYVLS--LSRMNISMDITPHSGISFSASQASAINSSLISHKIQFSPTL 258

Query: 326 FGRYQVLNVKYPGLPPSPPLAPSGTSSINDGLNTSNTNAGNAIKPLGVDVPRRKKEGLGR 385
            G Y++LN+ +    P+P  AP   SS +    +  ++A  +++      P +K+     
Sbjct: 259 VGDYKLLNLTW-FEAPAPSQAPLVASSPHKA-PSQGSSATTSVRS-----PGKKRH---P 318

Query: 386 NMIAVITISSFTALVMCVGLAWLCLLRCR----VSTHQPAQIPQNLIASPTKPPGTAGLI 445
           N+I + +I++   ++  + +  +C    R       H+ A  P+NL A            
Sbjct: 319 NLILIFSIAAGVLILAIITVLVICSRALREEKAPDPHKEAVKPRNLDA------------ 378

Query: 446 MVGSEPGSSSMPLDADPMTYIGAAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDD 505
             GS  GS   P     ++Y         +++++AT NF SA ILGEGGFG VY G L D
Sbjct: 379 --GSFGGSLPHPASTRFLSY---------EELKEATSNFESASILGEGGFGKVYRGILAD 438

Query: 506 GREVAVKVLKRHNQHGIREFLAEVEMLSRLHHRNLVKLIGICT--EDQIRCLVYELVPNG 565
           G  VA+K L      G +EF  E++MLSRLHHRNLVKL+G  +  +     L YELVPNG
Sbjct: 439 GTAVAIKKLTSGGPQGDKEFQVEIDMLSRLHHRNLVKLVGYYSSRDSSQHLLCYELVPNG 498

Query: 566 SVESHLHGIDKLTSPLDWDARMKIALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFT 625
           S+E+ LHG   L  PLDWD RMKIAL AARGLAYLHEDS P VIHRDFKASNILLE +F 
Sbjct: 499 SLEAWLHGPLGLNCPLDWDTRMKIALDAARGLAYLHEDSQPSVIHRDFKASNILLENNFN 558

Query: 626 PKVSDFGLARTALEEGNKHISTHVMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTG 685
            KV+DFGLA+ A E    H+ST VMGTFGY+APEYAMTGHLLVKSDVYSYGVVLLELLTG
Sbjct: 559 AKVADFGLAKQAPEGRGNHLSTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTG 618

Query: 686 RKPVDLSLPPGQENLVAWARPLLTSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPE 745
           RKPVD+S P GQENLV W RP+L  K+ L+ + D  ++     +   RV  IA+ CV PE
Sbjct: 619 RKPVDMSQPSGQENLVTWTRPVLRDKDRLEELVDSRLEGKYPKEDFIRVCTIAAACVAPE 668

Query: 746 VSHRPFMGEVVQALKLVCNEFEETNDPV 767
            S RP MGEVVQ+LK+V     E  DPV
Sbjct: 679 ASQRPTMGEVVQSLKMV-QRVVEYQDPV 668

BLAST of HG10023477 vs. TAIR 10
Match: AT5G13160.1 (Protein kinase superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 4.7e-86
Identity = 186/391 (47.57%), Postives = 238/391 (60.87%), Query Frame = 0

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLDD-GREVAVKVLKRHNQHGIREFL 522
           AA  F  +++  AT NF+    LGEGGFG VY G LD  G+ VAVK L R+   G REFL
Sbjct: 70  AAHTFAFRELAAATMNFHPDTFLGEGGFGRVYKGRLDSTGQVVAVKQLDRNGLQGNREFL 129

Query: 523 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 582
            EV MLS LHH NLV LIG C +   R LVYE +P GS+E HLH +      LDW+ RMK
Sbjct: 130 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMK 189

Query: 583 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 642
           IA GAA+GL +LH+ +NP VI+RDFK+SNILL+  F PK+SDFGLA+        H+ST 
Sbjct: 190 IAAGAAKGLEFLHDKANPPVIYRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTR 249

Query: 643 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 702
           VMGT+GY APEYAMTG L VKSDVYS+GVV LEL+TGRK +D  +P G++NLVAWARPL 
Sbjct: 250 VMGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDSEMPHGEQNLVAWARPLF 309

Query: 703 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 762
             +     + DP +K      +L +  A+ASMC+Q + + RP + +VV AL  + N+  +
Sbjct: 310 NDRRKFIKLADPRLKGRFPTRALYQALAVASMCIQEQAATRPLIADVVTALSYLANQAYD 369

Query: 763 TNDPVSRSYSRDE----LLSYMDSKFGGISGEILNAPETSHTFPSGKETNVGLSAS---D 822
            +   SR  +RDE    L++  D   GG SG   +  E S    S +ET   L+     +
Sbjct: 370 PSKDDSRR-NRDERGARLITRNDD--GGGSGSKFDL-EGSEKEDSPRETARILNRDINRE 429

Query: 823 LISASARFEGQELVSSRWHS--SNSEPLRTG 844
              A A+  G+ L   R  S    SE   TG
Sbjct: 430 RAVAEAKMWGESLREKRRQSEQGTSESNSTG 456

BLAST of HG10023477 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 1.2e-84
Identity = 165/336 (49.11%), Postives = 215/336 (63.99%), Query Frame = 0

Query: 463 AAKNFTLKDMEKATDNFNSARILGEGGFGIVYSGSLD-DGREVAVKVLKRHNQHGIREFL 522
           AA+ FT +++  AT NF    +LGEGGFG VY G L+  G+ VAVK L R+   G REFL
Sbjct: 67  AAQTFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFL 126

Query: 523 AEVEMLSRLHHRNLVKLIGICTEDQIRCLVYELVPNGSVESHLHGIDKLTSPLDWDARMK 582
            EV MLS LHH NLV LIG C +   R LVYE +P GS+E HLH +     PLDW  RM 
Sbjct: 127 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMT 186

Query: 583 IALGAARGLAYLHEDSNPRVIHRDFKASNILLEYDFTPKVSDFGLARTALEEGNKHISTH 642
           IA GAA+GL YLH+ +NP VI+RD K+SNILL   + PK+SDFGLA+        H+ST 
Sbjct: 187 IAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTR 246

Query: 643 VMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLSLPPGQENLVAWARPLL 702
           VMGT+GY APEYAMTG L +KSDVYS+GVV LEL+TGRK +D +  PG+ NLVAWARPL 
Sbjct: 247 VMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLF 306

Query: 703 TSKEGLDAITDPAIKPDISIDSLARVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFEE 762
             +     + DP+++    +  L +  A+A+MC+Q + + RP +G+VV AL  + ++  +
Sbjct: 307 KDRRKFPKMADPSLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFD 366

Query: 763 TNDPV---SRSYSRDELLSYMDSKFGGISGEILNAP 795
            N P    SRS S    +   D +     G  L++P
Sbjct: 367 PNAPSGQNSRSGSGPPFIRTRDDRRSLGDGSSLDSP 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899985.10.0e+0094.37receptor-like serine/threonine-protein kinase ALE2 [Benincasa hispida][more]
XP_004141683.10.0e+0092.34receptor-like serine/threonine-protein kinase ALE2 [Cucumis sativus] >KGN45527.1... [more]
XP_008462354.10.0e+0091.92PREDICTED: receptor-like serine/threonine-protein kinase ALE2 [Cucumis melo] >AD... [more]
KAA0059431.10.0e+0091.59receptor-like serine/threonine-protein kinase ALE2 [Cucumis melo var. makuwa] >T... [more]
XP_022154207.10.0e+0086.31receptor-like serine/threonine-protein kinase ALE2 isoform X2 [Momordica charant... [more]
Match NameE-valueIdentityDescription
Q8RWW01.3e-16849.72Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=37... [more]
Q6I5Q62.2e-8850.59Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9FE206.6e-8547.57Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=... [more]
Q1PDV61.6e-8349.11Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
Q9LDZ56.2e-8349.06Probable serine/threonine-protein kinase PBL21 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A0A0KAZ50.0e+0092.34Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G451... [more]
E5GCB10.0e+0091.92Protein kinase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3CGS90.0e+0091.92receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7V1350.0e+0091.59Receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo var. makuwa O... [more]
A0A6J1DL270.0e+0086.31receptor-like serine/threonine-protein kinase ALE2 isoform X2 OS=Momordica chara... [more]
Match NameE-valueIdentityDescription
AT5G56890.18.9e-21851.78Protein kinase superfamily protein [more]
AT2G20300.19.0e-17049.72Protein kinase superfamily protein [more]
AT4G02010.15.4e-10643.13Protein kinase superfamily protein [more]
AT5G13160.14.7e-8647.57Protein kinase superfamily protein [more]
AT5G18610.11.2e-8449.11Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 555..774
e-value: 5.6E-58
score: 197.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 450..554
e-value: 4.0E-35
score: 122.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..148
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..89
NoneNo IPR availablePANTHERPTHR47989:SF45OS01G0750732 PROTEINcoord: 23..873
NoneNo IPR availablePANTHERPTHR47989OS01G0750732 PROTEINcoord: 23..873
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 485..753
e-value: 2.09506E-99
score: 309.203
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 482..752
e-value: 6.8E-47
score: 159.9
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 485..507
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 601..613
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 479..760
score: 37.742741
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 459..752

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023477.1HG10023477.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity