HG10023470 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023470 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.2e-309
Identity = 516/574 (89.90%), Postives = 541/574 (94.25%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MASFSL FISFLC LFGHGV QLQASHHVYRAQ S+ +P+ YQQPYRTSYHFQPP NW+ 
Sbjct: 1   MASFSLCFISFLCFLFGHGVLQLQASHHVYRAQLSNSLPHHYQQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQNHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKINARGL IM+QSKDFVKWDQVDHPLHYADG+GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAKTG+ GVDT+V+ G VKHVLKVSLDDT+HD YT+G YDLEKDIYVPNK SI
Sbjct: 241 ECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKDIYVPNKGSI 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           EGY GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSV+DDI+KGWSG+QAIPRTIWLD
Sbjct: 301 EGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQAIPRTIWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVT AQADVEVSFKVKNFKKAQ
Sbjct: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSFKVKNFKKAQ 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
           +LKP+WKDPQLLC+Q GA TK GGVG FGLLVLAS DLKEYTS+SFTIFK++ KY+VLMC
Sbjct: 421 ILKPEWKDPQLLCSQKGATTK-GGVGSFGLLVLASKDLKEYTSISFTIFKRQGKYIVLMC 480

Query: 481 SDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN +NDKTTYGAFL+VDPV+EDLSLRSLIDHS+VESFGSKGKACITARVYPTL
Sbjct: 481 SDQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGKACITARVYPTL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           AIGD VGLY FNNGTESV ITKL AWSL KAQIN
Sbjct: 541 AIGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of HG10023470 vs. NCBI nr
Match: XP_004141685.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV1 [Cucumis sativus] >ALS30216.1 cell wall invertase 5 [Cucumis sativus])

HSP 1 Score: 1023.1 bits (2644), Expect = 9.4e-295
Identity = 494/578 (85.47%), Postives = 530/578 (91.70%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDY--QQPYRTSYHFQPPNNW 60
           MAS SL FISFLC LFGHGV+QLQASHHV++ QTS  +P  Y  +QPYRTSYHFQPP NW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  M---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDING 120
           +   NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQN 180
           CWSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQN
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 HINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSG 240
            IN+SSFRDPTTAWLG +GEWRVI+G+K++ RGL +MFQSKDF+KWDQVDHPLHYADGSG
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKR 300
           MWECPDF+PVAKTG+ GVDT V    VKHVLKVSLDDTRHDVYTIGTYD+EKDIYVPNK 
Sbjct: 241 MWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVPNKG 300

Query: 301 SIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIW 360
           SIEGY GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSV+DDI+KGWSG+Q IPRTIW
Sbjct: 301 SIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPRTIW 360

Query: 361 LDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKK 420
           LDASGKQLIQWPIEEIQKLRK+KV LTNKVLKKGS IEVKGVTP+QADVEVSFKVKN KK
Sbjct: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNLKK 420

Query: 421 AQMLKPQ-WKDPQLLCTQMG-AATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYV 480
           AQ+LKPQ W++PQ+LC+Q G AAT  GGVGPFGLLVLAS D KEYTSVSFTIFKK+ KYV
Sbjct: 421 AQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQSKYV 480

Query: 481 VLMCSDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARV 540
            LMCSDQSRSSLN +NDKTTYGAFLDVD  +EDLSLRSLIDHS+VESFG KGK CITARV
Sbjct: 481 ALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCITARV 540

Query: 541 YPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           YPTLAIGD VGLYAFNNGTESV ITKL+AWS+ KA+IN
Sbjct: 541 YPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of HG10023470 vs. NCBI nr
Match: KAG6575469.1 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.6e-294
Identity = 491/571 (85.99%), Postives = 527/571 (92.29%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWMN 60
           MAS SL FISFLC L  HG  +LQASHHVYRAQ S  + + Y+QPYRTSYHFQPP NW+N
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYRAQASRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGS 120
           GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGCWSGS
Sbjct: 61  GPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCWSGS 120

Query: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHINSS 180
           ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQN INSS
Sbjct: 121 ATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNQINSS 180

Query: 181 SFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMWECP 240
           SFRDPTTAWLG D EWRVIIGSKI+ARGL IMFQSKDFVKWDQVDHPLHYADG+GMWECP
Sbjct: 181 SFRDPTTAWLGPDSEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMWECP 240

Query: 241 DFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSIEGY 300
           DFFPVAK G++GVDT+V+   VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K SIEGY
Sbjct: 241 DFFPVAKNGRAGVDTSVSGRHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKGSIEGY 300

Query: 301 KGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLDASG 360
            GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSVDDDI+KGWSG+QAIPR +WLDASG
Sbjct: 301 TGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASG 360

Query: 361 KQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQMLK 420
           KQLIQWPI EIQKLRKDKVKLTNKVLKKGS+I VKGVT AQADV+VSFKVK+F KAQ LK
Sbjct: 361 KQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQPLK 420

Query: 421 PQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMCSDQ 480
            +WKDPQL+C+QMGAA K GGVGPFGLLVLAS +L+EYTSV F IFK++DKYVVLMCSDQ
Sbjct: 421 AEWKDPQLVCSQMGAAAK-GGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMCSDQ 480

Query: 481 SRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTLAIG 540
           SRSSLN +NDKTTYGAFLDVDPV E++SLRSLIDHSIVESFGSKGKACITARVYP LAIG
Sbjct: 481 SRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRLAIG 540

Query: 541 DKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           DK GLYAFNNGTESV ITKLSAWS+ KAQI+
Sbjct: 541 DKAGLYAFNNGTESVTITKLSAWSMKKAQIS 570

BLAST of HG10023470 vs. NCBI nr
Match: XP_022954011.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata] >XP_022954012.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita moschata])

HSP 1 Score: 1017.7 bits (2630), Expect = 3.9e-293
Identity = 491/574 (85.54%), Postives = 527/574 (91.81%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MAS SL FISFLC L  HG  +LQASHHVY  Q S  + + Y+QPYRTSYHFQPP NW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQNHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKI+ARGL IMFQSKDFVKWDQVDHPLHYADG+GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK  ++GVDT+V+   VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K SI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           EGY GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSVDDDI+KGWSG+QAIPR +WLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPI EIQKLRKDKVKLTNKVLKKGS+I VKGVT AQADV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
            LK +WKDPQL+C+QMGAA K GGVGPFGLLVLAS +L+EYTSV F IFK++DKYVVLMC
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAK-GGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMC 480

Query: 481 SDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN +NDKTTYGAFLDVDPV E++SLRSLIDHSIVESFGSKGKACITARVYP L
Sbjct: 481 SDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           AIGDK GLYAFNNGTESV ITKLSAWS+ KAQI+
Sbjct: 541 AIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of HG10023470 vs. NCBI nr
Match: XP_023547447.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.7e-291
Identity = 487/573 (84.99%), Postives = 522/573 (91.10%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MAS SL FISFLC L  HG  +LQASHHVY AQ S  + + ++QPYRTSYHFQPP NW+ 
Sbjct: 1   MASSSLCFISFLCFLLAHGALKLQASHHVYTAQASRLLLHHHEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQNHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKI+ARGL IMF+SKDFVKWDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK G +GVDT+V+   VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K SI
Sbjct: 241 ECPDFFPVAKNGHAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           E Y GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSVDDDI+KGWSG+QAIPR +WLD
Sbjct: 301 EDYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPI EIQKLRKDK+KLTNKVLKKGS++ VKGVT AQADV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLKKGSIVAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
            LK +WKDPQL+C+QMGAA K GGVGPFGLLVLAS +L+EYTSV F IFK +DKYVVLMC
Sbjct: 421 RLKAEWKDPQLVCSQMGAAAK-GGVGPFGLLVLASNNLEEYTSVFFRIFKGQDKYVVLMC 480

Query: 481 SDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN +NDKTTYGAFLDVDPV E++SLRSLIDHSIVESFGSKGKACITARVYP L
Sbjct: 481 SDQSRSSLNANNDKTTYGAFLDVDPVRENVSLRSLIDHSIVESFGSKGKACITARVYPRL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQI 570
           AIGDK GLYAFNNGTESV I KL AWSL KAQI
Sbjct: 541 AIGDKAGLYAFNNGTESVTIAKLRAWSLKKAQI 572

BLAST of HG10023470 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 5.6e-226
Identity = 385/582 (66.15%), Postives = 459/582 (78.87%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGVYQLQASHHVYR--AQTSHFVPNDYQQPYRTSYHFQPPNNWM 61
           ++  LW +  L LL G+ V  L+ASHHVY+   Q+++       QPYRT +HFQPP NWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 121
              NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNH 181
           WSGSATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW K P NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGM 241
           IN+SSFRDPTTAWLG+D +WRVIIGSKI+ RGL I + SKDF+KW++   PLHY DGSGM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKTGQSGVDTNVTRGP---VKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPN 301
           WECPDFFPV + G +GV+T+    P   +KHVLK+SLDDT+HD YTIGTYD  KD +VP+
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRT 361
                     RYDYGKYYASKTF+D  K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDASGKQLIQWPIEEIQKLRKDKVK-LTNKVLKKGSLIEVKGVTPAQADVEVSFKVKN 421
           IWLD SGKQLIQWP+ E+++LR  +VK L NKVLK GS +EV GVT AQADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKR--- 481
            +KA +++P W DPQL+C++M  + K+ G+GPFGL+VLAS +L+EYTSV F IFK R   
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKS-GLGPFGLMVLASKNLEEYTSVYFRIFKARQNS 486

Query: 482 DKYVVLMCSDQSRSSL-NSNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACI 541
           +KYVVLMCSDQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACI
Sbjct: 487 NKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACI 546

Query: 542 TARVYPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           T+RVYP LAIG    L+AFN G +SV +  L+AWS+N AQI+
Sbjct: 547 TSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of HG10023470 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 352/576 (61.11%), Postives = 432/576 (75.00%), Query Frame = 0

Query: 9   ISFLCLLFGHGVYQLQASHHVYRAQTSH--FVPNDYQ-----QPYRTSYHFQPPNNWM-- 68
           +S   +L  HG+  +     ++     H   + ND+Q      PYRT+YHFQ P NWM  
Sbjct: 11  LSISMMLLSHGMIIIAKDQAIHHHDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMND 70

Query: 69  -NGPMIYKGIYHLFYQYNPKGAVW-GNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 128
            NGPMIYKGIYHLFYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGCW
Sbjct: 71  PNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGCW 130

Query: 129 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMA--PTPQN 188
           SGS TILP +KP ILYTGIN KN QVQNLA+PKNLSDPYL+EW+K P+NPLMA  PT  N
Sbjct: 131 SGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNNN 190

Query: 189 HINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSG 248
           +IN+SSFRDP+TAW   DG+WRVI+G++   RGL ++F S DFVKW+   +PLH  +G+G
Sbjct: 191 NINASSFRDPSTAWQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGNG 250

Query: 249 MWECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKR 308
           +WECPDFFPV      G DT++    VKHVLK+SL DT+++ YTIG YD+EKDIYVP++ 
Sbjct: 251 IWECPDFFPVYVGKSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDEG 310

Query: 309 SIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIW 368
           SIE   GLRYDYGK+YASK+F+D +  RR+LWGWVNESS   DDI+KGWSG+QAIPRT+ 
Sbjct: 311 SIESDLGLRYDYGKFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTVV 370

Query: 369 LDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKK 428
           LD SGKQL+QWP+ E+  LR++ V+L ++V+K GSL+E+  +T +QADVE+SFK+     
Sbjct: 371 LDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESNY 430

Query: 429 AQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVL 488
            + L     +PQ+LC+Q GA+ K G  GPFGLL LAS  L+EYT+V F IFK  +KYVVL
Sbjct: 431 VEELDSTCTNPQILCSQKGASIK-GRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVL 490

Query: 489 MCSDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYP 548
           MCSDQ+RSSLN + DK ++G F+DVDP+ EDLSLR LIDHSIVESF +KGK+CITARVYP
Sbjct: 491 MCSDQTRSSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYP 550

Query: 549 TLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           T+AI DK  LY FNNGTE V ITKLSAWS+ KAQIN
Sbjct: 551 TMAINDKAKLYVFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of HG10023470 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 9.3e-205
Identity = 350/554 (63.18%), Postives = 423/554 (76.35%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYR---AQTSHFVPNDYQQPYRTSYHFQPPNN 60
           MA  S++ +S   L++   V  ++A+HHVY+     +SH       QPYRT+YHFQP  N
Sbjct: 1   MAISSIFLLSLFSLIY---VIPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKN 60

Query: 61  WM---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDIN 120
           W+   NGPM Y G YHLFYQYNPKGAVWGNIVWAHS S DL+NW P DHAI+PSQPSDI 
Sbjct: 61  WINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIK 120

Query: 121 GCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQ 180
           GCWSGSATILPG KPAILYTGI+P N QVQN+A+PKN+SDP LREW K PKNPLM PT  
Sbjct: 121 GCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIA 180

Query: 181 NHINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGS 240
           N INSSSFRDPTT+WLG+DG WRV+IGSKI+ +G+ I+++SK+FV W +  HPLH A+G+
Sbjct: 181 NKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGT 240

Query: 241 GMWECPDFFPVAKTG--QSGVDTNVT-RGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYV 300
           GMWECPDF+PV      ++GVDT+      V+HVLKVSLDDT+HD Y IG+YD+ KD++V
Sbjct: 241 GMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFV 300

Query: 301 PNKRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIP 360
           P     +    LRYDYGKYYASKTF+D  K RR+L GWVNESSSV DD++KGWSG+  IP
Sbjct: 301 PENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIP 360

Query: 361 RTIWLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVK 420
           R IWL  SGKQL+QWP++EI+ LR + V    KV+K G  I + GV   QADVE+SF++ 
Sbjct: 361 REIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEIS 420

Query: 421 NFKKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDK 480
           +  K + L+ +W DPQLLC+Q GA  K GGVGPFGLLV AS  LKEYT+V F IFK +DK
Sbjct: 421 DLGKVESLR-KWIDPQLLCSQKGAGVK-GGVGPFGLLVFASQGLKEYTAVFFRIFKYQDK 480

Query: 481 YVVLMCSDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITA 540
            +VLMCSDQSRSSLN  ND T+YG F+DVDP+ E LSLR+LIDHS+VESFG +G+AC+TA
Sbjct: 481 NLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTA 540

Query: 541 RVYPTLAIGDKVGL 545
           RVYPTLAI DK  L
Sbjct: 541 RVYPTLAIHDKAML 549

BLAST of HG10023470 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 713.8 bits (1841), Expect = 1.6e-204
Identity = 356/572 (62.24%), Postives = 416/572 (72.73%), Query Frame = 0

Query: 12  LCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM---NGPMIYKGI 71
           L +   + +  L+AS H            D  QPYRT YHFQP  NWM   NGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPGD 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGRDGEWRVIIGSKI-NARGLVIMFQSKDFVKWDQVDHPLHYADGSGMWECPDFFPVA 251
           AWLGRDGEWRVI+GS   + RGL I+++S+DF  W Q   PLHY D +GMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KTGQSGVDT-NVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSIEGYKGLRY 311
            TG  GV+T +V    +KHVLKVSL +T HD YTIG+YD EKD+YVP+   ++     R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLDASGKQLIQ 371
           DYGKYYASKTFYD  KKRR+LWGWVNESS   DDI+KGWSGLQ+ PR IWLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQMLKPQWKD 431
           WPIEEI+ LR  +V    KVLK GS ++V GVT AQADVEVSFKVK  +KA +++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFK------KRDKYVVLMCSD 491
           PQ +C+Q G  +   G+GPFGL+VLAS D++EYTSV F IFK      K+ KYVVLMCSD
Sbjct: 435 PQKICSQ-GDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSD 494

Query: 492 QSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTLAI 551
           QSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAI
Sbjct: 495 QSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAI 554

Query: 552 GDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           G+   L+ FN GT+SV I  LSAWSL  AQIN
Sbjct: 555 GENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of HG10023470 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 696.0 bits (1795), Expect = 3.4e-199
Identity = 340/543 (62.62%), Postives = 416/543 (76.61%), Query Frame = 0

Query: 38  VPNDYQ-QPYRTSYHFQPPNNWM---NGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHST 97
           VPN+ + QPYRT YHFQPP NWM   NGPMIYKGIYHLFYQ+N  GAV      VW H+T
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHAT 92

Query: 98  STDLINWEPHDHAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKN 157
           STDLINW     AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKN
Sbjct: 93  STDLINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKN 152

Query: 158 LSDPYLREWVKFPKNPLMAPTPQNHINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVI 217
           L+DPYLR W K P+NPL+ P+P NHINSS+FRDPTTAW GRDG WR+  GS+   RGL I
Sbjct: 153 LTDPYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAI 212

Query: 218 MFQSKDFVKWDQVDHPLHYADGSGMWECPDFFPVAKTGQSGVDTNVTRGP-VKHVLKVSL 277
           +  SKDFV W Q   PLHY DG+GMWECPDFFPVA+T   G+DT+ + GP VKHVLKVSL
Sbjct: 213 LHTSKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSL 272

Query: 278 DDTRHDVYTIGTYDLEKDIYVPNKRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWV 337
            DT +D YTIGTYD  +D+YVP+K  ++     RYDYGK+YASKTFYD   +RR+LWGWV
Sbjct: 273 TDTFNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWV 332

Query: 338 NESSSVDDDIQKGWSGLQAIPRTIWLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGS 397
           NESS   D+I+KGW+GLQAIPR +WLD SGK+L+QWP++EI++LR  +VK  NK+LK GS
Sbjct: 333 NESSPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGS 392

Query: 398 LIEVKGVTPAQADVEVSFKVKNF--KKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLL 457
           ++EV GVT  QADVEV FKV  F  +KA +++P W DPQL+C+Q  A++   G+GPFGL+
Sbjct: 393 VMEVHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS---GLGPFGLM 452

Query: 458 VLASTDLKEYTSVSFTIFKKRD---KYVVLMCSDQSRSSL-NSNDKTTYGAFLDVDPVTE 517
           VLAS +++EYTSV+  IF+  +   ++VV+MCSDQS SSL   NDKTTYGAFLD+ P  +
Sbjct: 453 VLASKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-Q 512

Query: 518 DLSLRSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTESVIITKLSAWSL 568
            +SLR+LID SIVESFG KGK CIT+RVYP LAIG++  L+AFN G+++V +  LSAWS+
Sbjct: 513 PISLRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSM 571

BLAST of HG10023470 vs. ExPASy TrEMBL
Match: A0A0A0K761 (Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1)

HSP 1 Score: 1023.1 bits (2644), Expect = 4.5e-295
Identity = 494/578 (85.47%), Postives = 530/578 (91.70%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDY--QQPYRTSYHFQPPNNW 60
           MAS SL FISFLC LFGHGV+QLQASHHV++ QTS  +P  Y  +QPYRTSYHFQPP NW
Sbjct: 1   MASISLCFISFLCFLFGHGVFQLQASHHVHKPQTSTSLPYHYHHRQPYRTSYHFQPPKNW 60

Query: 61  M---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDING 120
           +   NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDING
Sbjct: 61  INDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 120

Query: 121 CWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQN 180
           CWSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQN
Sbjct: 121 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 180

Query: 181 HINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSG 240
            IN+SSFRDPTTAWLG +GEWRVI+G+K++ RGL +MFQSKDF+KWDQVDHPLHYADGSG
Sbjct: 181 QINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYADGSG 240

Query: 241 MWECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKR 300
           MWECPDF+PVAKTG+ GVDT V    VKHVLKVSLDDTRHDVYTIGTYD+EKDIYVPNK 
Sbjct: 241 MWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVPNKG 300

Query: 301 SIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIW 360
           SIEGY GLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSV+DDI+KGWSG+Q IPRTIW
Sbjct: 301 SIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPRTIW 360

Query: 361 LDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKK 420
           LDASGKQLIQWPIEEIQKLRK+KV LTNKVLKKGS IEVKGVTP+QADVEVSFKVKN KK
Sbjct: 361 LDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKNLKK 420

Query: 421 AQMLKPQ-WKDPQLLCTQMG-AATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYV 480
           AQ+LKPQ W++PQ+LC+Q G AAT  GGVGPFGLLVLAS D KEYTSVSFTIFKK+ KYV
Sbjct: 421 AQILKPQYWENPQVLCSQKGRAATTKGGVGPFGLLVLASNDFKEYTSVSFTIFKKQSKYV 480

Query: 481 VLMCSDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARV 540
            LMCSDQSRSSLN +NDKTTYGAFLDVD  +EDLSLRSLIDHS+VESFG KGK CITARV
Sbjct: 481 ALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCITARV 540

Query: 541 YPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           YPTLAIGD VGLYAFNNGTESV ITKL+AWS+ KA+IN
Sbjct: 541 YPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of HG10023470 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.9e-293
Identity = 491/574 (85.54%), Postives = 527/574 (91.81%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MAS SL FISFLC L  HG  +LQASHHVY  Q S  + + Y+QPYRTSYHFQPP NW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHRYEQPYRTSYHFQPPKNWIN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQNHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSKI+ARGL IMFQSKDFVKWDQVDHPLHYADG+GMW
Sbjct: 181 NSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYADGTGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK  ++GVDT+V+   VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K SI
Sbjct: 241 ECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           EGY GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSVDDDI+KGWSG+QAIPR +WLD
Sbjct: 301 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPI EIQKLRKDKVKLTNKVLKKGS+I VKGVT AQADV+VSFKVK+F KAQ
Sbjct: 361 ASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKSFNKAQ 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
            LK +WKDPQL+C+QMGAA K GGVGPFGLLVLAS +L+EYTSV F IFK++DKYVVLMC
Sbjct: 421 PLKAEWKDPQLVCSQMGAAAK-GGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMC 480

Query: 481 SDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN +NDKTTYGAFLDVDPV E++SLRSLIDHSIVESFGSKGKACITARVYP L
Sbjct: 481 SDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPRL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           AIGDK GLYAFNNGTESV ITKLSAWS+ KAQI+
Sbjct: 541 AIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of HG10023470 vs. ExPASy TrEMBL
Match: A0A6J1JP62 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita maxima OX=3661 GN=LOC111488605 PE=3 SV=1)

HSP 1 Score: 1001.1 bits (2587), Expect = 1.8e-288
Identity = 478/574 (83.28%), Postives = 520/574 (90.59%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MAS SL FIS LC L  HG  +LQASHHVY AQ S  + + Y+QPYRTSYHFQPP NW+ 
Sbjct: 3   MASSSLCFISLLCFLLCHGALKLQASHHVYTAQASRLLLHHYEQPYRTSYHFQPPKNWIN 62

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGA WGNIVWAHSTSTDLINW+PHDHAIYPSQPSDINGCW
Sbjct: 63  DPNGPMIYKGIYHLFYQYNPKGATWGNIVWAHSTSTDLINWKPHDHAIYPSQPSDINGCW 122

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPG+KPAILYTGINPKNQQVQNLA+PKNLSDPYLREWVK P NPLMAPTPQN I
Sbjct: 123 SGSATILPGEKPAILYTGINPKNQQVQNLALPKNLSDPYLREWVKSPNNPLMAPTPQNRI 182

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DG+WRVIIGSKI+ARGL IMF+SKDFV+WDQVDHPLHYADG+GMW
Sbjct: 183 NSSSFRDPTTAWLGHDGQWRVIIGSKIDARGLAIMFRSKDFVRWDQVDHPLHYADGTGMW 242

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK G++G+DT+V  G VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K S 
Sbjct: 243 ECPDFFPVAKNGRAGIDTSVNGGHVKHVLKVSLDDTKHDHYTVGTYDLKKDIYVPDKEST 302

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           EGY GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSVDDD++KGWSG+QAIPR +WLD
Sbjct: 303 EGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDMKKGWSGVQAIPRAVWLD 362

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPI EIQKLRKDK+KL NKV+KKGS+I VKGVT AQADVEVSFKVK+FKKAQ
Sbjct: 363 ASGKQLIQWPIAEIQKLRKDKIKLRNKVVKKGSIIAVKGVTAAQADVEVSFKVKSFKKAQ 422

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
            LK +WKDPQL C++MGAA   GGVGPFGLLVLAS DL+EYTSV F +FK +DKYVVLMC
Sbjct: 423 RLKAEWKDPQLACSRMGAAAM-GGVGPFGLLVLASKDLEEYTSVFFRVFKGQDKYVVLMC 482

Query: 481 SDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN +NDKTTYGAFLDVDPV E++SLRSLIDHSIVESFGSKGKACITARVYP L
Sbjct: 483 SDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACITARVYPKL 542

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           A+GDK GLYAFNNGTE V ITKLSAWS+ KAQI+
Sbjct: 543 AMGDKAGLYAFNNGTERVTITKLSAWSMKKAQIS 575

BLAST of HG10023470 vs. ExPASy TrEMBL
Match: A0A6J1GPY1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456400 PE=3 SV=1)

HSP 1 Score: 1000.0 bits (2584), Expect = 4.1e-288
Identity = 481/573 (83.94%), Postives = 522/573 (91.10%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MAS SL FISFLC L  HG  +LQASHHVY  Q S  + + ++QPYRTS HFQPP NW+ 
Sbjct: 1   MASSSLCFISFLCFLLCHGALKLQASHHVYTPQPSRLLLHHHEQPYRTSCHFQPPKNWIN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AIYPSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVK PKNPLMAPTPQNHI
Sbjct: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLG DGEWRVIIGSK++ RGL IMF+SKDFVKWDQVDHPLHYADG GMW
Sbjct: 181 NSSSFRDPTTAWLGPDGEWRVIIGSKVDDRGLAIMFRSKDFVKWDQVDHPLHYADGMGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK G++GVDT+V+   VKHVLKVSLDDT+HD YT+GTYDL+KDIYVP+K SI
Sbjct: 241 ECPDFFPVAKNGRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVPDKGSI 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           + Y GLRYDYGKYYASKTF+DGQKKRRVLWGWVNESSSVDDDI+KGWSG+QAIPRT+WLD
Sbjct: 301 QDYSGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRTVWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQLIQWPI EIQKLRKDK+KLTNKVL+KGS+I VKGVT AQADV+VSFKVK+F KA 
Sbjct: 361 ASGKQLIQWPIAEIQKLRKDKIKLTNKVLEKGSIIAVKGVTAAQADVDVSFKVKSFNKAH 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
           +LK +WKDPQL+C+QMGAA K GGVGPFGLLVLAS +L+EYTSV F IFK++DKYVVLMC
Sbjct: 421 LLKAEWKDPQLVCSQMGAAAK-GGVGPFGLLVLASNNLEEYTSVFFRIFKRQDKYVVLMC 480

Query: 481 SDQSRSSLNS-NDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLN+ NDKT YGAFLDVDPV +++SLRSLIDHSIVESFGSKGKACITARVYP L
Sbjct: 481 SDQSRSSLNADNDKTMYGAFLDVDPVRKNVSLRSLIDHSIVESFGSKGKACITARVYPRL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQI 570
           AIGDK GLYAFNNGTESV ITKL AWSL KAQI
Sbjct: 541 AIGDKAGLYAFNNGTESVTITKLRAWSLKKAQI 572

BLAST of HG10023470 vs. ExPASy TrEMBL
Match: A0A6J1DM23 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Momordica charantia OX=3673 GN=LOC111021302 PE=3 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 8.0e-284
Identity = 471/574 (82.06%), Postives = 519/574 (90.42%), Query Frame = 0

Query: 1   MASFSLWFISFLCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM- 60
           MASFSLWF SFL +   HGV++L+ASHHVYRAQTS  VP D+QQPYRTSYHFQPP NWM 
Sbjct: 1   MASFSLWFFSFLSVFLCHGVFKLEASHHVYRAQTSASVPPDHQQPYRTSYHFQPPKNWMN 60

Query: 61  --NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCW 120
             NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINW PH HAI+PSQPSDINGCW
Sbjct: 61  DPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWVPHQHAIFPSQPSDINGCW 120

Query: 121 SGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHI 180
           SGSATILPG+KPA+LYTGINP+N QVQNLAVPKNLSDP+L EWVK PKNPLMAPTPQN+I
Sbjct: 121 SGSATILPGNKPAMLYTGINPQNHQVQNLAVPKNLSDPFLIEWVKSPKNPLMAPTPQNNI 180

Query: 181 NSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMW 240
           NSSSFRDPTTAWLGRD +WRV+IGSKINARGL IM++SKDF++WDQV++PLHYAD +GMW
Sbjct: 181 NSSSFRDPTTAWLGRDRQWRVVIGSKINARGLAIMYRSKDFIQWDQVNNPLHYADDTGMW 240

Query: 241 ECPDFFPVAKTGQSGVDTNVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSI 300
           ECPDFFPVAK  + GVDT+V    VKHVLKVSLDDT+HD YT+GTY+++KD+YVP+K S+
Sbjct: 241 ECPDFFPVAKNSRVGVDTSVNGPRVKHVLKVSLDDTKHDHYTVGTYNVKKDVYVPDKGSV 300

Query: 301 EGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLD 360
           EGY GLRYDYGK YASKTF+D +KKRRVLWGWVNESSSVDDD +KGWSG+QAIPR+IWLD
Sbjct: 301 EGYAGLRYDYGKCYASKTFFDSEKKRRVLWGWVNESSSVDDDKKKGWSGVQAIPRSIWLD 360

Query: 361 ASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQ 420
           ASGKQL+QWPI EIQKLRKDKVKL NKVLKKGS+IEVKGVT AQ DVEVSFKVKNFKKAQ
Sbjct: 361 ASGKQLVQWPIVEIQKLRKDKVKLINKVLKKGSVIEVKGVTAAQVDVEVSFKVKNFKKAQ 420

Query: 421 MLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKRDKYVVLMC 480
           +LKP+WKDPQLLC Q GAA K  GVGPFGLL+LAS DLKEYTSV F +FK+  KYVVLMC
Sbjct: 421 VLKPEWKDPQLLCAQKGAA-KESGVGPFGLLLLASKDLKEYTSVFFKVFKREGKYVVLMC 480

Query: 481 SDQSRSSLNS-NDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTL 540
           SDQSRSSLNS NDKTTYGAF+D+DPV E+LSLRSLIDHSIVESFG KGKACITARVYPTL
Sbjct: 481 SDQSRSSLNSNNDKTTYGAFVDMDPVRENLSLRSLIDHSIVESFGVKGKACITARVYPTL 540

Query: 541 AIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           AIG+ VGLYAFNN TE V I+KL+AWSLNKA IN
Sbjct: 541 AIGENVGLYAFNNATEGVTISKLTAWSLNKAHIN 573

BLAST of HG10023470 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 790.4 bits (2040), Expect = 9.5e-229
Identity = 385/579 (66.49%), Postives = 459/579 (79.27%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGVYQLQASHHVYR--AQTSHFVPNDYQQPYRTSYHFQPPNNWM 61
           ++  LW +  L LL G+ V  L+ASHHVY+   Q+++       QPYRT +HFQPP NWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSG 121
           NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGCWSG
Sbjct: 67  NGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 126

Query: 122 SATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHINS 181
           SATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW K P NPLMAP   N IN+
Sbjct: 127 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINA 186

Query: 182 SSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGMWEC 241
           SSFRDPTTAWLG+D +WRVIIGSKI+ RGL I + SKDF+KW++   PLHY DGSGMWEC
Sbjct: 187 SSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWEC 246

Query: 242 PDFFPVAKTGQSGVDTNVTRGP---VKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRS 301
           PDFFPV + G +GV+T+    P   +KHVLK+SLDDT+HD YTIGTYD  KD +VP+   
Sbjct: 247 PDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGF 306

Query: 302 IEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWL 361
                  RYDYGKYYASKTF+D  K RR+LWGW NESSSV+DD++KGWSG+Q IPR IWL
Sbjct: 307 KMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWL 366

Query: 362 DASGKQLIQWPIEEIQKLRKDKVK-LTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKK 421
           D SGKQLIQWP+ E+++LR  +VK L NKVLK GS +EV GVT AQADVEV FKV++ +K
Sbjct: 367 DRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEK 426

Query: 422 AQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKR---DKY 481
           A +++P W DPQL+C++M  + K+ G+GPFGL+VLAS +L+EYTSV F IFK R   +KY
Sbjct: 427 ADVIEPSWTDPQLICSKMNVSVKS-GLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKY 486

Query: 482 VVLMCSDQSRSSL-NSNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITAR 541
           VVLMCSDQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACIT+R
Sbjct: 487 VVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSR 546

Query: 542 VYPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           VYP LAIG    L+AFN G +SV +  L+AWS+N AQI+
Sbjct: 547 VYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of HG10023470 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 785.0 bits (2026), Expect = 4.0e-227
Identity = 385/582 (66.15%), Postives = 459/582 (78.87%), Query Frame = 0

Query: 2   ASFSLWFISFLCLLFGHGVYQLQASHHVYR--AQTSHFVPNDYQQPYRTSYHFQPPNNWM 61
           ++  LW +  L LL G+ V  L+ASHHVY+   Q+++       QPYRT +HFQPP NWM
Sbjct: 7   SNIGLWLL--LTLLIGNYVVNLEASHHVYKRLTQSTNTKSPSVNQPYRTGFHFQPPKNWM 66

Query: 62  ---NGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGC 121
              NGPMIYKGIYHLFYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AI+PS P DINGC
Sbjct: 67  NDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGC 126

Query: 122 WSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNH 181
           WSGSATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW K P NPLMAP   N 
Sbjct: 127 WSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNG 186

Query: 182 INSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQSKDFVKWDQVDHPLHYADGSGM 241
           IN+SSFRDPTTAWLG+D +WRVIIGSKI+ RGL I + SKDF+KW++   PLHY DGSGM
Sbjct: 187 INASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGM 246

Query: 242 WECPDFFPVAKTGQSGVDTNVTRGP---VKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPN 301
           WECPDFFPV + G +GV+T+    P   +KHVLK+SLDDT+HD YTIGTYD  KD +VP+
Sbjct: 247 WECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPD 306

Query: 302 KRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRT 361
                     RYDYGKYYASKTF+D  K RR+LWGW NESSSV+DD++KGWSG+Q IPR 
Sbjct: 307 NGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRK 366

Query: 362 IWLDASGKQLIQWPIEEIQKLRKDKVK-LTNKVLKKGSLIEVKGVTPAQADVEVSFKVKN 421
           IWLD SGKQLIQWP+ E+++LR  +VK L NKVLK GS +EV GVT AQADVEV FKV++
Sbjct: 367 IWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRD 426

Query: 422 FKKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFKKR--- 481
            +KA +++P W DPQL+C++M  + K+ G+GPFGL+VLAS +L+EYTSV F IFK R   
Sbjct: 427 LEKADVIEPSWTDPQLICSKMNVSVKS-GLGPFGLMVLASKNLEEYTSVYFRIFKARQNS 486

Query: 482 DKYVVLMCSDQSRSSL-NSNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACI 541
           +KYVVLMCSDQSRSSL   NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+ACI
Sbjct: 487 NKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACI 546

Query: 542 TARVYPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           T+RVYP LAIG    L+AFN G +SV +  L+AWS+N AQI+
Sbjct: 547 TSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of HG10023470 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.1e-205
Identity = 356/572 (62.24%), Postives = 416/572 (72.73%), Query Frame = 0

Query: 12  LCLLFGHGVYQLQASHHVYRAQTSHFVPNDYQQPYRTSYHFQPPNNWM---NGPMIYKGI 71
           L +   + +  L+AS H            D  QPYRT YHFQP  NWM   NGPMIYKGI
Sbjct: 15  LSMFLANFITDLEASSH-----------QDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGI 74

Query: 72  YHLFYQYNPKGAVWG-NIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSATILPGD 131
           YHLFYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDINGCWSGS TILP  
Sbjct: 75  YHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSGSVTILPNG 134

Query: 132 KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTPQNHINSSSFRDPTT 191
           KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM     N IN   FRDPTT
Sbjct: 135 KPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTT 194

Query: 192 AWLGRDGEWRVIIGSKI-NARGLVIMFQSKDFVKWDQVDHPLHYADGSGMWECPDFFPVA 251
           AWLGRDGEWRVI+GS   + RGL I+++S+DF  W Q   PLHY D +GMWECPDFFPV+
Sbjct: 195 AWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVS 254

Query: 252 KTGQSGVDT-NVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYVPNKRSIEGYKGLRY 311
            TG  GV+T +V    +KHVLKVSL +T HD YTIG+YD EKD+YVP+   ++     R 
Sbjct: 255 ITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPDLGFVQNESAPRL 314

Query: 312 DYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIPRTIWLDASGKQLIQ 371
           DYGKYYASKTFYD  KKRR+LWGWVNESS   DDI+KGWSGLQ+ PR IWLD SGK+L+Q
Sbjct: 315 DYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQ 374

Query: 372 WPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVKNFKKAQMLKPQWKD 431
           WPIEEI+ LR  +V    KVLK GS ++V GVT AQADVEVSFKVK  +KA +++P W D
Sbjct: 375 WPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWTD 434

Query: 432 PQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFK------KRDKYVVLMCSD 491
           PQ +C+Q G  +   G+GPFGL+VLAS D++EYTSV F IFK      K+ KYVVLMCSD
Sbjct: 435 PQKICSQ-GDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSD 494

Query: 492 QSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKGKACITARVYPTLAI 551
           QSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAI
Sbjct: 495 QSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAI 554

Query: 552 GDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           G+   L+ FN GT+SV I  LSAWSL  AQIN
Sbjct: 555 GENANLFVFNKGTQSVDILTLSAWSLKSAQIN 574

BLAST of HG10023470 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 701.4 bits (1809), Expect = 5.8e-202
Identity = 340/540 (62.96%), Postives = 416/540 (77.04%), Query Frame = 0

Query: 38  VPNDYQ-QPYRTSYHFQPPNNWMNGPMIYKGIYHLFYQYNPKGAVW--GNIVWAHSTSTD 97
           VPN+ + QPYRT YHFQPP NWMNGPMIYKGIYHLFYQ+N  GAV      VW H+TSTD
Sbjct: 33  VPNNIKNQPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTD 92

Query: 98  LINWEPHDHAIYPSQPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSD 157
           LINW     AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKNL+D
Sbjct: 93  LINWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTD 152

Query: 158 PYLREWVKFPKNPLMAPTPQNHINSSSFRDPTTAWLGRDGEWRVIIGSKINARGLVIMFQ 217
           PYLR W K P+NPL+ P+P NHINSS+FRDPTTAW GRDG WR+  GS+   RGL I+  
Sbjct: 153 PYLRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHT 212

Query: 218 SKDFVKWDQVDHPLHYADGSGMWECPDFFPVAKTGQSGVDTNVTRGP-VKHVLKVSLDDT 277
           SKDFV W Q   PLHY DG+GMWECPDFFPVA+T   G+DT+ + GP VKHVLKVSL DT
Sbjct: 213 SKDFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDT 272

Query: 278 RHDVYTIGTYDLEKDIYVPNKRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNES 337
            +D YTIGTYD  +D+YVP+K  ++     RYDYGK+YASKTFYD   +RR+LWGWVNES
Sbjct: 273 FNDYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNES 332

Query: 338 SSVDDDIQKGWSGLQAIPRTIWLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIE 397
           S   D+I+KGW+GLQAIPR +WLD SGK+L+QWP++EI++LR  +VK  NK+LK GS++E
Sbjct: 333 SPEKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVME 392

Query: 398 VKGVTPAQADVEVSFKVKNF--KKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLA 457
           V GVT  QADVEV FKV  F  +KA +++P W DPQL+C+Q  A++   G+GPFGL+VLA
Sbjct: 393 VHGVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS---GLGPFGLMVLA 452

Query: 458 STDLKEYTSVSFTIFKKRD---KYVVLMCSDQSRSSL-NSNDKTTYGAFLDVDPVTEDLS 517
           S +++EYTSV+  IF+  +   ++VV+MCSDQS SSL   NDKTTYGAFLD+ P  + +S
Sbjct: 453 SKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISPY-QPIS 512

Query: 518 LRSLIDHSIVESFGSKGKACITARVYPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKA 568
           LR+LID SIVESFG KGK CIT+RVYP LAIG++  L+AFN G+++V +  LSAWS+  +
Sbjct: 513 LRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of HG10023470 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 689.1 bits (1777), Expect = 3.0e-198
Identity = 338/526 (64.26%), Postives = 396/526 (75.29%), Query Frame = 0

Query: 55  PNNWMNGPMIYKGIYHLFYQYNPKGAVWG-NIVWAHSTSTDLINWEPHDHAIYPSQPSDI 114
           PN  ++GPMIYKGIYHLFYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDI
Sbjct: 35  PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94

Query: 115 NGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKFPKNPLMAPTP 174
           NGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    
Sbjct: 95  NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154

Query: 175 QNHINSSSFRDPTTAWLGRDGEWRVIIGSKI-NARGLVIMFQSKDFVKWDQVDHPLHYAD 234
            N IN   FRDPTTAWLGRDGEWRVI+GS   + RGL I+++S+DF  W Q   PLHY D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214

Query: 235 GSGMWECPDFFPVAKTGQSGVDT-NVTRGPVKHVLKVSLDDTRHDVYTIGTYDLEKDIYV 294
            +GMWECPDFFPV+ TG  GV+T +V    +KHVLKVSL +T HD YTIG+YD EKD+YV
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274

Query: 295 PNKRSIEGYKGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVDDDIQKGWSGLQAIP 354
           P+   ++     R DYGKYYASKTFYD  KKRR+LWGWVNESS   DDI+KGWSGLQ+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334

Query: 355 RTIWLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTPAQADVEVSFKVK 414
           R IWLD SGK+L+QWPIEEI+ LR  +V    KVLK GS ++V GVT AQADVEVSFKVK
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394

Query: 415 NFKKAQMLKPQWKDPQLLCTQMGAATKNGGVGPFGLLVLASTDLKEYTSVSFTIFK---- 474
             +KA +++P W DPQ +C+Q G  +   G+GPFGL+VLAS D++EYTSV F IFK    
Sbjct: 395 ELEKADVIEPSWTDPQKICSQ-GDLSVMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDD 454

Query: 475 --KRDKYVVLMCSDQSRSSLN-SNDKTTYGAFLDVDPVTEDLSLRSLIDHSIVESFGSKG 534
             K+ KYVVLMCSDQSRSSLN  NDK+T+GAF+ +DP  + +SLR+LIDHSIVES+G  G
Sbjct: 455 TNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGG 514

Query: 535 KACITARVYPTLAIGDKVGLYAFNNGTESVIITKLSAWSLNKAQIN 571
           + CIT+RVYP LAIG+   L+ FN GT+SV I  LSAWSL  AQIN
Sbjct: 515 RTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559