HG10023464 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10023464
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	mitochondrial import inner membrane translocase subunit TIM23-2-like
Location	Chr05: 34456315 .. 34456887 (+)
RNA-Seq Expression	HG10023464
Synteny	HG10023464

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGACCATTCTTCCAATCGCGATTCTGATTCAGGGTTTGATCATGGCTCATCCCAACCGCGTCTATACAATCCCTACAAGGATCTCCAGGTTCCTTATCGAAACTTCCAGCTTCCAACCTCCCCAGAGTTTCTTTTCGATGAAGAAGCTCGCCGCCAGCGTCGATCTTGGGGCGAGAACCTCACCTTCTACACTGGTTGCGGTTACCTCGCTGGTGCAGTGGGCGGCGCGTCTACAGGACTCGTTTCTGGCGTTAAATCTTTTGAATCAGGTGATACTATGAAACTCCGGATCAATCGGATCCTGAACTCGTCTGGTCATTCAGGGCGTCTCTGGGGGAATCGGCTCGGCGTGATTGGGCTGCTATATGCTGGTTTGGAGAGCGGGATTGAGGCGGTGAGGGATACGGACGATGTGTGGAACTGTGTTGCAGCAGGACTTGGCACTGGTGCGCTTTATCGTGCAGCAAGAGGGGTGAGATCGGCGGCGGTGGCCGGTGCTATTGGCGGTGTCGTGGTTGGCGTGGCGGTGACGGGTAAGCAGATGTTGAAGCGGTACGTGCCGATATGA

mRNA sequence

ATGGCGGACCATTCTTCCAATCGCGATTCTGATTCAGGGTTTGATCATGGCTCATCCCAACCGCGTCTATACAATCCCTACAAGGATCTCCAGGTTCCTTATCGAAACTTCCAGCTTCCAACCTCCCCAGAGTTTCTTTTCGATGAAGAAGCTCGCCGCCAGCGTCGATCTTGGGGCGAGAACCTCACCTTCTACACTGGTTGCGGTTACCTCGCTGGTGCAGTGGGCGGCGCGTCTACAGGACTCGTTTCTGGCGTTAAATCTTTTGAATCAGGTGATACTATGAAACTCCGGATCAATCGGATCCTGAACTCGTCTGGTCATTCAGGGCGTCTCTGGGGGAATCGGCTCGGCGTGATTGGGCTGCTATATGCTGGTTTGGAGAGCGGGATTGAGGCGGTGAGGGATACGGACGATGTGTGGAACTGTGTTGCAGCAGGACTTGGCACTGGTGCGCTTTATCGTGCAGCAAGAGGGGTGAGATCGGCGGCGGTGGCCGGTGCTATTGGCGGTGTCGTGGTTGGCGTGGCGGTGACGGGTAAGCAGATGTTGAAGCGGTACGTGCCGATATGA

Coding sequence (CDS)

ATGGCGGACCATTCTTCCAATCGCGATTCTGATTCAGGGTTTGATCATGGCTCATCCCAACCGCGTCTATACAATCCCTACAAGGATCTCCAGGTTCCTTATCGAAACTTCCAGCTTCCAACCTCCCCAGAGTTTCTTTTCGATGAAGAAGCTCGCCGCCAGCGTCGATCTTGGGGCGAGAACCTCACCTTCTACACTGGTTGCGGTTACCTCGCTGGTGCAGTGGGCGGCGCGTCTACAGGACTCGTTTCTGGCGTTAAATCTTTTGAATCAGGTGATACTATGAAACTCCGGATCAATCGGATCCTGAACTCGTCTGGTCATTCAGGGCGTCTCTGGGGGAATCGGCTCGGCGTGATTGGGCTGCTATATGCTGGTTTGGAGAGCGGGATTGAGGCGGTGAGGGATACGGACGATGTGTGGAACTGTGTTGCAGCAGGACTTGGCACTGGTGCGCTTTATCGTGCAGCAAGAGGGGTGAGATCGGCGGCGGTGGCCGGTGCTATTGGCGGTGTCGTGGTTGGCGTGGCGGTGACGGGTAAGCAGATGTTGAAGCGGTACGTGCCGATATGA

Protein sequence

MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGENLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQMLKRYVPI

Homology

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BLAST of HG10023464 vs. NCBI nr
Match: XP_038896585.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Benincasa hispida])

HSP 1 Score: 372.5 bits (955), Expect = 2.2e-99
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MAD SSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADRSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDT+KLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTLKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG
Sbjct: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

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BLAST of HG10023464 vs. NCBI nr
Match: KAG6575462.1 (Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014007.1 Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 371.3 bits (952), Expect = 4.9e-99
Identity = 186/190 (97.89%), Postives = 188/190 (98.95%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MADHSSNRD+DSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADHSSNRDTDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTG VSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGFVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESG EAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG+AVTG
Sbjct: 121 GLLYAGLESGFEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGMAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

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BLAST of HG10023464 vs. NCBI nr
Match: XP_022953829.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita moschata] >XP_022953830.1 mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita moschata] >XP_023548834.1 mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita pepo subsp. pepo] >XP_023548835.1 mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.0 bits (946), Expect = 2.4e-98
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MADHSSNRD+DSGFDH SSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADHSSNRDTDSGFDHSSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTG VSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGFVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESG EAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG+AVTG
Sbjct: 121 GLLYAGLESGFEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGMAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

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BLAST of HG10023464 vs. NCBI nr
Match: XP_004141690.1 (mitochondrial import inner membrane translocase subunit TIM23-1 [Cucumis sativus] >XP_008462339.1 PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-1-like [Cucumis melo] >KAA0059418.1 mitochondrial import inner membrane translocase subunit TIM23-1 [Cucumis melo var. makuwa] >KAE8646509.1 hypothetical protein Csa_016542 [Cucumis sativus] >TYK03908.1 mitochondrial import inner membrane translocase subunit TIM23-1 [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-97
Identity = 184/190 (96.84%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MA HSSNRD+DS FDH SSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MAVHSSNRDTDSEFDHSSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGA+GGVVVG+AVTG
Sbjct: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAVGGVVVGLAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. NCBI nr
Match: XP_022992412.1 (mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita maxima])

HSP 1 Score: 364.4 bits (934), Expect = 6.0e-97
Identity = 183/190 (96.32%), Postives = 185/190 (97.37%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MADHSS RD+DSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADHSSKRDTDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAV GASTG VSGVKSFESGDTMKLRINRILNSSGH GRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVAGASTGFVSGVKSFESGDTMKLRINRILNSSGHLGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESG EAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG+AVTG
Sbjct: 121 GLLYAGLESGFEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGMAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. ExPASy Swiss-Prot
Match: Q38820 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis thaliana OX=3702 GN=TIM23-2 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 2.8e-65
Identity = 126/181 (69.61%), Postives = 147/181 (81.22%), Query Frame = 0


Query: 15  DHGSSQ-PRLYNPYKDLQVPYRN----FQLPTSPEFLFDEEARRQRRSWGENLTFYTGCG 74
           DHGS +  RLYNPY++ +VP       ++LPTSPEFLF EEA RQRRSWGENLTFYTG  
Sbjct: 8   DHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTA 67

Query: 75  YLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLES 134
           YL G+V GAS G+++GVKSFESGDT KL+INRILNSSG +GR WGNR+G+IGL+YAG+ES
Sbjct: 68  YLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIES 127

Query: 135 GIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQMLKRYVP 191
           GI A  D DDVW  V AGLGTGA+ RAARGVRSAAVAGA+GG+  G  V GKQ++KRYVP
Sbjct: 128 GIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVP 187

[Close]

BLAST of HG10023464 vs. ExPASy Swiss-Prot
Match: Q9LNQ1 (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis thaliana OX=3702 GN=TIM23-1 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 7.0e-64
Identity = 123/187 (65.78%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 7   NRDSDSGFDHGSSQPRLYNPYKDLQVPYRN---FQLPTSPEFLFDEEARRQRRSWGENLT 66
           NR SD   D  +   RLY+PY++ QVP ++   ++LPTSPEFLF EE+ +QRRSWGENLT
Sbjct: 4   NRSSDHESDENT---RLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLT 63

Query: 67  FYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLL 126
           FYTG GYLAG+V GAS G+ SG+KSFE+GDT KL+INRILNSSG +GR WGNR+G++GL+
Sbjct: 64  FYTGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLI 123

Query: 127 YAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQM 186
           YAG+ESG+ AV D DDVW  V AGLGTGA++RAARGVRSAAVAGA GG+  G  V GKQ+
Sbjct: 124 YAGIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQV 183

Query: 187 LKRYVPI 191
            KRY  I
Sbjct: 184 FKRYAHI 187

[Close]

BLAST of HG10023464 vs. ExPASy Swiss-Prot
Match: Q9S837 (Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis thaliana OX=3702 GN=TIM23-3 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 5.6e-45
Identity = 94/171 (54.97%), Postives = 126/171 (73.68%), Query Frame = 0


Query: 22  RLYNPYKDLQVPYRN-FQLPTSPEFLFDEEARRQRRSWGENLTFYTGCGYLAGAVGGAST 81
           R YNPY+ + +PYR  ++LPTSPEFLF+EEA ++R +WGENLTF+TG GY  G+V GA  
Sbjct: 18  RQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGWGYCTGSVLGAFK 77

Query: 82  GLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLESGIEAVRDTDD- 141
           G ++G+++ E G+++K+R NRILNS G   R  GN LG +GL++A +ESG+  +RD DD 
Sbjct: 78  GTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAMESGVTYMRDGDDG 137

Query: 142 VWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQMLKRYVPI 191
               V AGL TG LYRAA G RSA VAGA+GGV    AV G++++KR+VPI
Sbjct: 138 SLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRFVPI 188

[Close]

BLAST of HG10023464 vs. ExPASy Swiss-Prot
Match: Q9USM7 (Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tim23 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.9e-13
Identity = 47/127 (37.01%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 56  RSWGENLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGN 115
           R W ++L + TG  YL+G   G   GL  G+K  +   + +LR+N ILN     G   GN
Sbjct: 77  RGWQDDLCYGTGTSYLSGLAIGGLWGLNEGMKKTKDITSTRLRLNGILNGVTRRGPFVGN 136

Query: 116 RLGVIGLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG 175
            LGV+ L+Y G+ S I   R      N VAAG  TGALY++ RG+R+ A++ ++     G
Sbjct: 137 SLGVLALVYNGINSLIGYKRQKHGWENSVAAGALTGALYKSTRGLRAMAISSSLVATAAG 196

Query: 176 VAVTGKQ 183
           +    K+
Sbjct: 197 IWTLAKR 203

[Close]

BLAST of HG10023464 vs. ExPASy Swiss-Prot
Match: A4IFL0 (Mitochondrial import inner membrane translocase subunit Tim23 OS=Bos taurus OX=9913 GN=TIMM23 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-10
Identity = 54/150 (36.00%), Postives = 73/150 (48.67%), Query Frame = 0


Query: 33  PYRN----FQLPTSPEFLFDEEARRQRRSWGENLTFYT--GCGYLAGAVGGASTGLVSGV 92
           PY N    + +  + EF+    A + R  +   L F+T  GC  + GA  GA  GL  G+
Sbjct: 44  PYLNVDPRYLVQDTDEFILPTGANKTRGRF--ELAFFTIGGC-CMTGAAFGAMNGLRLGL 103

Query: 93  KSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLESGIEAVRDTDDVWNCVAA 152
           K  ++    K R  +ILN     G LW N LG + LLY+     IE  R  +D  N VAA
Sbjct: 104 KETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNTVAA 163

Query: 153 GLGTGALYRAARGVRSAAVAGAIGGVVVGV 177
           G  TG LY+   G+R AA  G  G  + G+
Sbjct: 164 GTMTGMLYKCTGGLRGAARGGLAGLTLTGL 190

[Close]

BLAST of HG10023464 vs. ExPASy TrEMBL
Match: A0A6J1GQS1 (mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbita moschata OX=3662 GN=LOC111456245 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.2e-98
Identity = 185/190 (97.37%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MADHSSNRD+DSGFDH SSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADHSSNRDTDSGFDHSSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTG VSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGFVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESG EAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG+AVTG
Sbjct: 121 GLLYAGLESGFEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGMAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. ExPASy TrEMBL
Match: A0A5D3BY19 (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001040 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.7e-97
Identity = 184/190 (96.84%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MA HSSNRD+DS FDH SSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MAVHSSNRDTDSEFDHSSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGA+GGVVVG+AVTG
Sbjct: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAVGGVVVGLAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. ExPASy TrEMBL
Match: A0A1S3CGP4 (mitochondrial import inner membrane translocase subunit TIM23-1-like OS=Cucumis melo OX=3656 GN=LOC103500713 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.7e-97
Identity = 184/190 (96.84%), Postives = 187/190 (98.42%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MA HSSNRD+DS FDH SSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MAVHSSNRDTDSEFDHSSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGA+GGVVVG+AVTG
Sbjct: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAVGGVVVGLAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. ExPASy TrEMBL
Match: A0A6J1JPR4 (mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbita maxima OX=3661 GN=LOC111488726 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.9e-97
Identity = 183/190 (96.32%), Postives = 185/190 (97.37%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MADHSS RD+DSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADHSSKRDTDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAV GASTG VSGVKSFESGDTMKLRINRILNSSGH GRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVAGASTGFVSGVKSFESGDTMKLRINRILNSSGHLGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESG EAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVG+AVTG
Sbjct: 121 GLLYAGLESGFEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGMAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. ExPASy TrEMBL
Match: A0A6J1KID3 (mitochondrial import inner membrane translocase subunit TIM23-1-like OS=Cucurbita maxima OX=3661 GN=LOC111494717 PE=4 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.5e-97
Identity = 184/190 (96.84%), Postives = 186/190 (97.89%), Query Frame = 0


Query: 1   MADHSSNRDSDSGFDHGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60
           MAD SS+R+ DSGFD GSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE
Sbjct: 1   MADRSSDREIDSGFDQGSSQPRLYNPYKDLQVPYRNFQLPTSPEFLFDEEARRQRRSWGE 60

Query: 61  NLTFYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120
           NLTFYTGCGYLAGAVGGASTG VSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI
Sbjct: 61  NLTFYTGCGYLAGAVGGASTGFVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVI 120

Query: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180
           GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG
Sbjct: 121 GLLYAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTG 180

Query: 181 KQMLKRYVPI 191
           KQMLKRYVPI
Sbjct: 181 KQMLKRYVPI 190

[Close]

BLAST of HG10023464 vs. TAIR 10
Match: AT1G72750.1 (translocase inner membrane subunit 23-2 )

HSP 1 Score: 249.6 bits (636), Expect = 2.0e-66
Identity = 126/181 (69.61%), Postives = 147/181 (81.22%), Query Frame = 0


Query: 15  DHGSSQ-PRLYNPYKDLQVPYRN----FQLPTSPEFLFDEEARRQRRSWGENLTFYTGCG 74
           DHGS +  RLYNPY++ +VP       ++LPTSPEFLF EEA RQRRSWGENLTFYTG  
Sbjct: 8   DHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTA 67

Query: 75  YLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLES 134
           YL G+V GAS G+++GVKSFESGDT KL+INRILNSSG +GR WGNR+G+IGL+YAG+ES
Sbjct: 68  YLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIES 127

Query: 135 GIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQMLKRYVP 191
           GI A  D DDVW  V AGLGTGA+ RAARGVRSAAVAGA+GG+  G  V GKQ++KRYVP
Sbjct: 128 GIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVP 187

[Close]

BLAST of HG10023464 vs. TAIR 10
Match: AT1G17530.1 (translocase of inner mitochondrial membrane 23 )

HSP 1 Score: 245.0 bits (624), Expect = 5.0e-65
Identity = 123/187 (65.78%), Postives = 149/187 (79.68%), Query Frame = 0


Query: 7   NRDSDSGFDHGSSQPRLYNPYKDLQVPYRN---FQLPTSPEFLFDEEARRQRRSWGENLT 66
           NR SD   D  +   RLY+PY++ QVP ++   ++LPTSPEFLF EE+ +QRRSWGENLT
Sbjct: 4   NRSSDHESDENT---RLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLT 63

Query: 67  FYTGCGYLAGAVGGASTGLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLL 126
           FYTG GYLAG+V GAS G+ SG+KSFE+GDT KL+INRILNSSG +GR WGNR+G++GL+
Sbjct: 64  FYTGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLI 123

Query: 127 YAGLESGIEAVRDTDDVWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQM 186
           YAG+ESG+ AV D DDVW  V AGLGTGA++RAARGVRSAAVAGA GG+  G  V GKQ+
Sbjct: 124 YAGIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQV 183

Query: 187 LKRYVPI 191
            KRY  I
Sbjct: 184 FKRYAHI 187

[Close]

BLAST of HG10023464 vs. TAIR 10
Match: AT3G04800.1 (translocase inner membrane subunit 23-3 )

HSP 1 Score: 182.2 bits (461), Expect = 3.9e-46
Identity = 94/171 (54.97%), Postives = 126/171 (73.68%), Query Frame = 0


Query: 22  RLYNPYKDLQVPYRN-FQLPTSPEFLFDEEARRQRRSWGENLTFYTGCGYLAGAVGGAST 81
           R YNPY+ + +PYR  ++LPTSPEFLF+EEA ++R +WGENLTF+TG GY  G+V GA  
Sbjct: 18  RQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGWGYCTGSVLGAFK 77

Query: 82  GLVSGVKSFESGDTMKLRINRILNSSGHSGRLWGNRLGVIGLLYAGLESGIEAVRDTDD- 141
           G ++G+++ E G+++K+R NRILNS G   R  GN LG +GL++A +ESG+  +RD DD 
Sbjct: 78  GTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAMESGVTYMRDGDDG 137

Query: 142 VWNCVAAGLGTGALYRAARGVRSAAVAGAIGGVVVGVAVTGKQMLKRYVPI 191
               V AGL TG LYRAA G RSA VAGA+GGV    AV G++++KR+VPI
Sbjct: 138 SLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRFVPI 188

The following BLAST results are available for this feature:

BLAST of HG10023464 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896585.1	2.2e-99	98.95	mitochondrial import inner membrane translocase subunit TIM23-2-like [Benincasa ...	[more]
KAG6575462.1	4.9e-99	97.89	Mitochondrial import inner membrane translocase subunit TIM23-1, partial [Cucurb...	[more]
XP_022953829.1	2.4e-98	97.37	mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ...	[more]
XP_004141690.1	3.5e-97	96.84	mitochondrial import inner membrane translocase subunit TIM23-1 [Cucumis sativus...	[more]
XP_022992412.1	6.0e-97	96.32	mitochondrial import inner membrane translocase subunit TIM23-2-like [Cucurbita ...	[more]

BLAST of HG10023464 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38820	2.8e-65	69.61	Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis t...	[more]
Q9LNQ1	7.0e-64	65.78	Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis t...	[more]
Q9S837	5.6e-45	54.97	Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis t...	[more]
Q9USM7	1.9e-13	37.01	Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharom...	[more]
A4IFL0	1.5e-10	36.00	Mitochondrial import inner membrane translocase subunit Tim23 OS=Bos taurus OX=9...	[more]

BLAST of HG10023464 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQS1	1.2e-98	97.37	mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbit...	[more]
A0A5D3BY19	1.7e-97	96.84	Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Cucumis melo ...	[more]
A0A1S3CGP4	1.7e-97	96.84	mitochondrial import inner membrane translocase subunit TIM23-1-like OS=Cucumis ...	[more]
A0A6J1JPR4	2.9e-97	96.32	mitochondrial import inner membrane translocase subunit TIM23-2-like OS=Cucurbit...	[more]
A0A6J1KID3	6.5e-97	96.84	mitochondrial import inner membrane translocase subunit TIM23-1-like OS=Cucurbit...	[more]

BLAST of HG10023464 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72750.1	2.0e-66	69.61	translocase inner membrane subunit 23-2	[more]
AT1G17530.1	5.0e-65	65.78	translocase of inner mitochondrial membrane 23	[more]
AT3G04800.1	3.9e-46	54.97	translocase inner membrane subunit 23-3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 64..173 e-value: 1.9E-24 score: 86.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR15371	TIM23	coord: 2..190
None	No IPR available	PANTHER	PTHR15371:SF25	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM23-2-LIKE	coord: 2..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10023464.1	HG10023464.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030150	protein import into mitochondrial matrix
cellular_component	GO:0031305	integral component of mitochondrial inner membrane
cellular_component	GO:0005744	TIM23 mitochondrial import inner membrane translocase complex
molecular_function	GO:0008320	protein transmembrane transporter activity