HG10023463 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023463 vs. NCBI nr
Match: XP_038896758.1 (U-box domain-containing protein 35-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1340.9 bits (3469), Expect = 0.0e+00
Identity = 702/799 (87.86%), Postives = 733/799 (91.74%), Query Frame = 0

Query: 1   MATPMAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLH 60
           M TP+AEEET +SHST+HTVIAISRDKNSQ AVKWAVEHL NRKN GDCILIHVR QSLH
Sbjct: 1   METPVAEEETNDSHSTAHTVIAISRDKNSQYAVKWAVEHLLNRKNTGDCILIHVRDQSLH 60

Query: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSI 120
           PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVP+ALIDYVS HSI
Sbjct: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPSALIDYVSKHSI 120

Query: 121 SNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180
           SN+VVGASSRNAIL+KFK+PDV T LLKS PESCSVYVIARGKVHTKRLRKRHKSQTDAR
Sbjct: 121 SNIVVGASSRNAILRKFKNPDVPTSLLKSTPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180

Query: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDT 240
           ETRTRTPQIGRSLSKIQKSAYSNISGLSED  RDSSGTSNDSMSGISDFSGPLSFKSIDT
Sbjct: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDINRDSSGTSNDSMSGISDFSGPLSFKSIDT 240

Query: 241 SFESTDFSL-TSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQK 300
           SFE+TDFSL TSSE S+RSFVSSNTPPSIESEMRKLR E+K+TMEMYDSASKEAV+AKQK
Sbjct: 241 SFENTDFSLTTSSENSSRSFVSSNTPPSIESEMRKLRFEIKRTMEMYDSASKEAVIAKQK 300

Query: 301 AKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQ 360
           AKELQQLKMEMEEDRK E +KSAEE L ALAEFEKQKNKAATEAAL+AQKLAELETQK++
Sbjct: 301 AKELQQLKMEMEEDRKNEHSKSAEETLTALAEFEKQKNKAATEAALMAQKLAELETQKQR 360

Query: 361 IITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQA 420
           IITEEKARIEAEERKK MELFE SNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQ 
Sbjct: 361 IITEEKARIEAEERKKTMELFEHSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQG 420

Query: 421 LLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGS 480
            LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYE+MENGS
Sbjct: 421 SLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEFMENGS 480

Query: 481 LEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSK 540
           LEDRLF+KD+TPPIPWRTRF+IASDIATALLFLHQMKPEPVVHRDLKPANILLD NYVSK
Sbjct: 481 LEDRLFQKDNTPPIPWRTRFKIASDIATALLFLHQMKPEPVVHRDLKPANILLDQNYVSK 540

Query: 541 IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI 600
           IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI
Sbjct: 541 IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI 600

Query: 601 TARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRT 660
           TARSPMGLSYQVEEAIEHG+FPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLR+
Sbjct: 601 TARSPMGLSYQVEEAIEHGRFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRS 660

Query: 661 VLLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESE------------- 720
           VLLPELVRL+N+GSGEMPPKGKLPNV K  DQ A+NSAP+LKGSESE             
Sbjct: 661 VLLPELVRLRNLGSGEMPPKGKLPNVNKPHDQWAANSAPILKGSESEIYVRKRWQRLKAN 720

Query: 721 -----------------IKKKWSWNNSDAKVLNIPLPISGKGVDVLSKKQQ--SSERCWS 767
                            IKKKWSW+NSDA VLN+PLP+SGK VDV+SKKQQ  SSERCWS
Sbjct: 721 TRFWSQKGIGLNTVSPQIKKKWSWSNSDANVLNMPLPMSGKRVDVMSKKQQQSSSERCWS 780

BLAST of HG10023463 vs. NCBI nr
Match: XP_008462336.1 (PREDICTED: U-box domain-containing protein 35-like isoform X1 [Cucumis melo])

HSP 1 Score: 1264.6 bits (3271), Expect = 0.0e+00
Identity = 664/789 (84.16%), Postives = 703/789 (89.10%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEA 64
           MAEEETI+S+S +HT+IAISRDKNSQ AVKWAVEHLFNRKN GDCILIHVRSQ LHPQEA
Sbjct: 1   MAEEETIDSYSNNHTMIAISRDKNSQYAVKWAVEHLFNRKNTGDCILIHVRSQGLHPQEA 60

Query: 65  DTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVV 124
           D++PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID+VS HSISN+V
Sbjct: 61  DSIPKEYRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHVSKHSISNIV 120

Query: 125 VGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETRT 184
           VGAS+RN I++KFK+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  E++T
Sbjct: 121 VGASNRNPIMRKFKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMNESQT 180

Query: 185 RTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFES 244
            TP++G SLSK +KSAYS+ISGLSEDK RDSSGTSNDSMSGISDFSGPLSFKSI+TSFE+
Sbjct: 181 GTPRVGSSLSKTRKSAYSSISGLSEDKYRDSSGTSNDSMSGISDFSGPLSFKSIETSFEN 240

Query: 245 TDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQ 304
            DFS +SSETSTRS+VSS+TPPSIESEM++LR ELKQ MEMYDSASKEA VAKQKAKEL+
Sbjct: 241 LDFSPSSSETSTRSYVSSSTPPSIESEMKRLRFELKQMMEMYDSASKEAAVAKQKAKELE 300

Query: 305 QLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEE 364
           QLKME EED KIER KSA+E L ALAEFEKQKNKAA EA LVAQKLAELETQKKQIITEE
Sbjct: 301 QLKMEKEEDDKIERGKSADEALTALAEFEKQKNKAAAEATLVAQKLAELETQKKQIITEE 360

Query: 365 KARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHT 424
           KARIEAEERKK MELFERSNICYRRYSIDEIE ATDHFNESNKIGEGGYGPVYQALLDHT
Sbjct: 361 KARIEAEERKKTMELFERSNICYRRYSIDEIEVATDHFNESNKIGEGGYGPVYQALLDHT 420

Query: 425 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 484
           SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL
Sbjct: 421 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 480

Query: 485 FRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 544
           FRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG
Sbjct: 481 FRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 540

Query: 545 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 604
           LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP
Sbjct: 541 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 600

Query: 605 MGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPE 664
           MGLSYQVEEAIEH KFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL+PE
Sbjct: 601 MGLSYQVEEAIEHDKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLMPE 660

Query: 665 LVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI--KKKWS--------WN 724
           L RLKN+G GE PPK KL NV KA DQ   NSAPVLKGSESEI  +K+W         W+
Sbjct: 661 LSRLKNLGLGETPPKSKLANVSKAHDQQPPNSAPVLKGSESEIYVRKRWQRLQVSTRFWS 720

Query: 725 NSDAKVLNI-----------PLPISGKGVDVLSKKQ------QSSERCWSFVSCASAATS 767
                V  I            +PISGK VDV+ KKQ      QSS+RCWSF SCASAA  
Sbjct: 721 QKGIGVNTISSQIKKRWSIPSIPISGKRVDVMKKKQQQQQHLQSSQRCWSFASCASAANM 780

BLAST of HG10023463 vs. NCBI nr
Match: XP_038896760.1 (U-box domain-containing protein 35-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 655/716 (91.48%), Postives = 683/716 (95.39%), Query Frame = 0

Query: 1   MATPMAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLH 60
           M TP+AEEET +SHST+HTVIAISRDKNSQ AVKWAVEHL NRKN GDCILIHVR QSLH
Sbjct: 1   METPVAEEETNDSHSTAHTVIAISRDKNSQYAVKWAVEHLLNRKNTGDCILIHVRDQSLH 60

Query: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSI 120
           PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVP+ALIDYVS HSI
Sbjct: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPSALIDYVSKHSI 120

Query: 121 SNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180
           SN+VVGASSRNAIL+KFK+PDV T LLKS PESCSVYVIARGKVHTKRLRKRHKSQTDAR
Sbjct: 121 SNIVVGASSRNAILRKFKNPDVPTSLLKSTPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180

Query: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDT 240
           ETRTRTPQIGRSLSKIQKSAYSNISGLSED  RDSSGTSNDSMSGISDFSGPLSFKSIDT
Sbjct: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDINRDSSGTSNDSMSGISDFSGPLSFKSIDT 240

Query: 241 SFESTDFSL-TSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQK 300
           SFE+TDFSL TSSE S+RSFVSSNTPPSIESEMRKLR E+K+TMEMYDSASKEAV+AKQK
Sbjct: 241 SFENTDFSLTTSSENSSRSFVSSNTPPSIESEMRKLRFEIKRTMEMYDSASKEAVIAKQK 300

Query: 301 AKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQ 360
           AKELQQLKMEMEEDRK E +KSAEE L ALAEFEKQKNKAATEAAL+AQKLAELETQK++
Sbjct: 301 AKELQQLKMEMEEDRKNEHSKSAEETLTALAEFEKQKNKAATEAALMAQKLAELETQKQR 360

Query: 361 IITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQA 420
           IITEEKARIEAEERKK MELFE SNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQ 
Sbjct: 361 IITEEKARIEAEERKKTMELFEHSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQG 420

Query: 421 LLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGS 480
            LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYE+MENGS
Sbjct: 421 SLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEFMENGS 480

Query: 481 LEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSK 540
           LEDRLF+KD+TPPIPWRTRF+IASDIATALLFLHQMKPEPVVHRDLKPANILLD NYVSK
Sbjct: 481 LEDRLFQKDNTPPIPWRTRFKIASDIATALLFLHQMKPEPVVHRDLKPANILLDQNYVSK 540

Query: 541 IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI 600
           IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI
Sbjct: 541 IGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLI 600

Query: 601 TARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRT 660
           TARSPMGLSYQVEEAIEHG+FPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLR+
Sbjct: 601 TARSPMGLSYQVEEAIEHGRFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRS 660

Query: 661 VLLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEIKKKWSWNN 716
           VLLPELVRL+N+GSGEMPPKGKLPNV K  DQ A+NSAP+LKGSESE   + + NN
Sbjct: 661 VLLPELVRLRNLGSGEMPPKGKLPNVNKPHDQWAANSAPILKGSESEEGMRNNPNN 716

BLAST of HG10023463 vs. NCBI nr
Match: XP_038896759.1 (U-box domain-containing protein 35-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 649/738 (87.94%), Postives = 678/738 (91.87%), Query Frame = 0

Query: 62  QEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSIS 121
           +EADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVP+ALIDYVS HSIS
Sbjct: 33  EEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPSALIDYVSKHSIS 92

Query: 122 NVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARE 181
           N+VVGASSRNAIL+KFK+PDV T LLKS PESCSVYVIARGKVHTKRLRKRHKSQTDARE
Sbjct: 93  NIVVGASSRNAILRKFKNPDVPTSLLKSTPESCSVYVIARGKVHTKRLRKRHKSQTDARE 152

Query: 182 TRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTS 241
           TRTRTPQIGRSLSKIQKSAYSNISGLSED  RDSSGTSNDSMSGISDFSGPLSFKSIDTS
Sbjct: 153 TRTRTPQIGRSLSKIQKSAYSNISGLSEDINRDSSGTSNDSMSGISDFSGPLSFKSIDTS 212

Query: 242 FESTDFSL-TSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKA 301
           FE+TDFSL TSSE S+RSFVSSNTPPSIESEMRKLR E+K+TMEMYDSASKEAV+AKQKA
Sbjct: 213 FENTDFSLTTSSENSSRSFVSSNTPPSIESEMRKLRFEIKRTMEMYDSASKEAVIAKQKA 272

Query: 302 KELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQI 361
           KELQQLKMEMEEDRK E +KSAEE L ALAEFEKQKNKAATEAAL+AQKLAELETQK++I
Sbjct: 273 KELQQLKMEMEEDRKNEHSKSAEETLTALAEFEKQKNKAATEAALMAQKLAELETQKQRI 332

Query: 362 ITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQAL 421
           ITEEKARIEAEERKK MELFE SNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQ  
Sbjct: 333 ITEEKARIEAEERKKTMELFEHSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQGS 392

Query: 422 LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSL 481
           LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYE+MENGSL
Sbjct: 393 LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEFMENGSL 452

Query: 482 EDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI 541
           EDRLF+KD+TPPIPWRTRF+IASDIATALLFLHQMKPEPVVHRDLKPANILLD NYVSKI
Sbjct: 453 EDRLFQKDNTPPIPWRTRFKIASDIATALLFLHQMKPEPVVHRDLKPANILLDQNYVSKI 512

Query: 542 GDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 601
           GDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT
Sbjct: 513 GDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 572

Query: 602 ARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTV 661
           ARSPMGLSYQVEEAIEHG+FPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLR+V
Sbjct: 573 ARSPMGLSYQVEEAIEHGRFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRSV 632

Query: 662 LLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESE-------------- 721
           LLPELVRL+N+GSGEMPPKGKLPNV K  DQ A+NSAP+LKGSESE              
Sbjct: 633 LLPELVRLRNLGSGEMPPKGKLPNVNKPHDQWAANSAPILKGSESEIYVRKRWQRLKANT 692

Query: 722 ----------------IKKKWSWNNSDAKVLNIPLPISGKGVDVLSKKQQ--SSERCWSF 767
                           IKKKWSW+NSDA VLN+PLP+SGK VDV+SKKQQ  SSERCWSF
Sbjct: 693 RFWSQKGIGLNTVSPQIKKKWSWSNSDANVLNMPLPMSGKRVDVMSKKQQQSSSERCWSF 752

BLAST of HG10023463 vs. NCBI nr
Match: XP_011659638.1 (U-box domain-containing protein 35 isoform X1 [Cucumis sativus])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 649/791 (82.05%), Postives = 694/791 (87.74%), Query Frame = 0

Query: 1   MATPMAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLH 60
           MATPMAEEE+ +S+ST++TVIAISRDKNS  AVKWAVEHLFNRKN G+C+LIHVR+QSLH
Sbjct: 1   MATPMAEEESFDSYSTNNTVIAISRDKNSHYAVKWAVEHLFNRKNTGECVLIHVRTQSLH 60

Query: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSI 120
           PQE DT+PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID++S HSI
Sbjct: 61  PQEVDTIPKEFRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHISKHSI 120

Query: 121 SNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180
           SN+VVGAS+RN I++K K+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  
Sbjct: 121 SNIVVGASNRNPIMRKLKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMT 180

Query: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDT 240
           ET+T TP+I  SLSK QKSAYS+ISG SEDK R SSGTSNDS SGISDFSGPLSFKSI+T
Sbjct: 181 ETQTGTPRISGSLSKTQKSAYSSISGQSEDKYRYSSGTSNDSTSGISDFSGPLSFKSIET 240

Query: 241 SFESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKA 300
           SFE+ DFSL+SSETSTRS+VSS+TPP+IE EM+KLR ELK+ MEMYDSA KEA VAKQKA
Sbjct: 241 SFENQDFSLSSSETSTRSYVSSSTPPTIEYEMKKLRFELKKMMEMYDSACKEAAVAKQKA 300

Query: 301 KELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQI 360
           KEL+ LKME EED KIE  KS  E L  LAEFEKQKNKA  EA LVAQKLAELETQKK+I
Sbjct: 301 KELRHLKMEKEEDNKIECGKSTYEALTTLAEFEKQKNKAEAEATLVAQKLAELETQKKRI 360

Query: 361 ITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQAL 420
           ITEEKARIEAEERKK MELFERSNICYRR+SID+IE ATDHFNESNKIGEGGYGPVYQAL
Sbjct: 361 ITEEKARIEAEERKKTMELFERSNICYRRFSIDQIEVATDHFNESNKIGEGGYGPVYQAL 420

Query: 421 LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSL 480
           L+HTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHP+MVLLLGACPEYGCLVYEYMENGSL
Sbjct: 421 LEHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPNMVLLLGACPEYGCLVYEYMENGSL 480

Query: 481 EDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI 540
           EDRLFRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI
Sbjct: 481 EDRLFRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI 540

Query: 541 GDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 600
           GDVGLARLVPP+VADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT
Sbjct: 541 GDVGLARLVPPTVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 600

Query: 601 ARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTV 660
           ARSPMGLSYQVEEAIEHGKFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDL TV
Sbjct: 601 ARSPMGLSYQVEEAIEHGKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLCTV 660

Query: 661 LLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI--KKKWS------ 720
           L+PEL RLKN+GSGE PPK KL  V +A DQ   NSAPVLKGSE EI  +K+W       
Sbjct: 661 LMPELSRLKNLGSGETPPKSKLATVSRAHDQQVLNSAPVLKGSEGEIYVRKRWERLKVST 720

Query: 721 --WNNSDAKVLN-----------IP-LPISGKGVDVLSKKQQS---SERCWSFVSCASAA 767
             W+      LN           IP +PISGK VD   KKQQ    SERCWSF SCASAA
Sbjct: 721 RFWSQKGGIGLNTISSQIKKKWSIPSIPISGKRVDGSKKKQQPQQISERCWSFGSCASAA 780

BLAST of HG10023463 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 1.1e-104
Identity = 267/735 (36.33%), Postives = 387/735 (52.65%), Query Frame = 0

Query: 20  VIAISRDKNSQSAVKWAVEHLFNRKNAGDCIL-IHVRSQSLHPQEADTVP-KECRPPTEA 79
           V+A+S    S+  V WA+E      N G  +L IH    S+     + +P  E R     
Sbjct: 23  VVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDVVT 82

Query: 80  ELHQFFL--------PYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVVVGASS 139
              Q  L        PY     R+ +  + ++I   +V  A+ + V+  SI  +V+G SS
Sbjct: 83  AYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGGSS 142

Query: 140 RNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETRTRTPQI 199
           R+   +K    D+ + +   +P  C+VYV+++GK+   R      + T   +   RT   
Sbjct: 143 RSFFSRK---ADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSERTNSS 202

Query: 200 GRS------LSKIQKSAYSNIS-------------------------------------G 259
             S       S +  SA+ + S                                      
Sbjct: 203 SGSSGPTSDSSDVMSSAHDSQSRPLSLPVRRMQHFPAIAGQASVPMETSSVGSDETRCMS 262

Query: 260 LSEDKIRDSSG---TSNDSMS--------------GISDFSGPLSFKSIDTSFE------ 319
           L  ++ RD S    +S D+ S               +S  S    + +  T F       
Sbjct: 263 LDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNFGTRFSWSGMGV 322

Query: 320 STDFSLTSSETSTRSFVSS-----NTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQ 379
            T  S  S + S  S   S     +   ++  E+ KLR EL+   EMY  A  E   A +
Sbjct: 323 DTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYAVAQTETFDASR 382

Query: 380 KAKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKK 439
           K  EL Q +  +EE  K+E  K  E   R LAE EKQ  + A   A   ++ AE E  ++
Sbjct: 383 KLGELNQRR--LEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRERAEREIAQR 442

Query: 440 QIITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQ 499
           +    + AR + +E++K         + Y+ ++ +EI AAT  F+E  KIG G YG VY+
Sbjct: 443 REAERKSAR-DTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGMGAYGAVYK 502

Query: 500 ALLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENG 559
             L HT+  +K+L+   +   +QFQQE+E+LS++RHPH+VLLLGACPE G LVYEYMENG
Sbjct: 503 CNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGALVYEYMENG 562

Query: 560 SLEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVS 619
           SLEDRLF+ +++PP+PW  RFRIA ++A AL+FLH+ KP+P++HRDLKPANILLDHN+VS
Sbjct: 563 SLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPANILLDHNFVS 622

Query: 620 KIGDVGLARLVP-PSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQ 672
           K+GDVGL+ +V    ++   T Y  T+  GT CYIDPEYQ+TG +  KSDIYSFG++LLQ
Sbjct: 623 KVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQ 682

BLAST of HG10023463 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.8e-103
Identity = 269/826 (32.57%), Postives = 412/826 (49.88%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLH---- 64
           ++E  ++    +    +AI+  K S+  V WA+E  F  +   D  L++VR    +    
Sbjct: 10  LSEHLSLPPPPSPSVAVAINGKKKSKYVVFWALEK-FIPEGFTDFKLLYVRPPVSYIPTP 69

Query: 65  -------PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID 124
                   +  + V    +   +   ++   PY+    R+ +  + +++  ++   A+ +
Sbjct: 70  MGIAVAVSELREDVVSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAE 129

Query: 125 YVSNHSISNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKR----- 184
            ++   ++ +V+G S R    +K    D+S+ +  ++P  C+VYVI++GK+ + R     
Sbjct: 130 EIAGTGVTKLVIGMSLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESD 189

Query: 185 ----LRKRHKSQTDARETRTRTPQIGRSLSKIQKSAYSNI-------------------- 244
               +R    S T       R P   +        A S +                    
Sbjct: 190 ASGSIRFERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMD 249

Query: 245 ---------------------SGLSEDKIRDSSGTSN--------DSMSGISDFSGPLSF 304
                                SG+   K +D S +++        +SMS  S +      
Sbjct: 250 TSSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDR 309

Query: 305 KSIDTSFESTDFSLTSSE------------TSTRSFVS---------SNTPPSIESEMRK 364
           + + +S  S +  L + +            + T S +S         ++   ++  E+ K
Sbjct: 310 REMRSSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEK 369

Query: 365 LRLELKQTMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEK 424
           LR ELK   EMY  A  E V A +K  EL Q +   EE  K+   K  EEV +  A  EK
Sbjct: 370 LRAELKHVQEMYAMAQTETVGASKKLTELNQRR--FEESEKLVELKEKEEVAKDTASKEK 429

Query: 425 QKNKAATEAALVAQKLAELETQKKQIITEEKARIEAEERKKAMELFERSNICYRRYSIDE 484
           Q+ + A + A   ++L   E   ++   E KA  +A E+ K         + Y+ Y+ +E
Sbjct: 430 QRYEEAMKEAEKVKELMMKEALHRR-EAEFKAERDAREKDKLQASLVSPGVQYQHYTWEE 489

Query: 485 IEAATDHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRH 544
           I AAT  F E+ KIG G YG VY+  L HT+ A+K+L    +   +QF QE+E+LS++RH
Sbjct: 490 IAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRH 549

Query: 545 PHMVLLLGACPEYGCLVYEYMENGSLEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQ 604
           PH+VLLLGACPE GCLVYEYM+NGSL+DRL   +DTPPIPW  RFRIA ++A+AL+FLH+
Sbjct: 550 PHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASALVFLHK 609

Query: 605 MKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDP 664
            KP P++HRDLKP NILLDHN+VSK+GDVGL+ +V      S T +  T+  GT CYIDP
Sbjct: 610 SKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDP 669

Query: 665 EYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVEEAI-EHGKFPQILDPAITDWPI 724
           EYQ+TG++  KSD+YS GV++LQLITA+  + +++ VEEAI +  +F  ILD     WPI
Sbjct: 670 EYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPI 729

Query: 725 EDTLGLAQLALKCCELRKRDRPDLRTVLLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLA 727
            DT  LA L L C E+R+RDRPDL+  ++P L RL+ +         + P+    P    
Sbjct: 730 SDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPS--GPPSHFI 789

BLAST of HG10023463 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 374.0 bits (959), Expect = 4.0e-102
Identity = 245/702 (34.90%), Postives = 384/702 (54.70%), Query Frame = 0

Query: 26  DKNSQSAVKWAVEHLFNRKNAGDCILIHVR---SQSLHPQEADTVPKECRPPTEAELHQF 85
           +  ++  V+WA++   ++++     L+HV+   S S+     D      +   + +  + 
Sbjct: 16  NSKTKGVVRWALQEFASQEHV-VFKLLHVQPRDSNSVSTTRKDLTTSVYKKDVDRKTREM 75

Query: 86  FLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVVVGASSRNAILKKFKSPDV 145
            LP R     + +    +++   D+ +A+   V +H IS +V+GASS      K K  ++
Sbjct: 76  LLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSIIFSWKLKRSNL 135

Query: 146 STCLLKSIPESCSVYVIARGK---VHTKRLRKRHKSQTDARETR---------------- 205
           S+ +  + P  CSV+VI++GK   V    +        D  E+R                
Sbjct: 136 SSRIADATPRFCSVHVISKGKLLNVRKSDMDTETSIADDRSESRFSSDSHSGTVSSTSSH 195

Query: 206 --TRTP---QIGRSLSKIQKSAYSNIS-----------------GLSEDKIRDSSGTSND 265
             + TP   Q  ++L+ + +   +NI                   + E K+ +  G    
Sbjct: 196 QFSSTPLLFQRIQALTTVNQKVGTNIGKQNNEPHHHHHNRAGSLDVDESKLLNQKGFYRT 255

Query: 266 SMSGISDFSGPLSFKSIDTSFESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQ 325
           S SGI      +S      S+ S+     SS ++     SS++    + E+ KL++EL+ 
Sbjct: 256 SSSGIGYGGSDIS------SWRSSQMEEASSSSTYSDPTSSSSQIHKDFELEKLKIELRH 315

Query: 326 TMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAAT 385
              MY  A  E + A +K ++L Q +   EE  +++     EE    + E E+++ + A 
Sbjct: 316 IKGMYAVAQSEVIDASKKMQDLNQRR--SEEATRLKNLTIREEEADEVVEMERERQEDAE 375

Query: 386 EAALVAQKLAELETQKK--QIITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAAT 445
             A + ++  E ET+++       E+ R E +  + A+E        Y ++  +EI  AT
Sbjct: 376 NEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEAT 435

Query: 446 DHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVL 505
             F++  KIG GGYG VY+  L HT+VA+K+L  D+S   +QF QE+E+LS++RHPH++L
Sbjct: 436 SSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLL 495

Query: 506 LLGACPEYGCLVYEYMENGSLEDRLFRK------DDTPPIPWRTRFRIASDIATALLFLH 565
           LLGACPE G LVYEYM NGSLE+RL ++         PP+ W  RFRIA +IA+AL FLH
Sbjct: 496 LLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLH 555

Query: 566 QMKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPSVADSVTQYHMTAAAGTFCYID 625
             +P P+VHRDLKPANILLD N VSKIGDVGL+++V    + + T ++ T   GTF YID
Sbjct: 556 TNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYID 615

Query: 626 PEYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVEEAI--EHGKFPQILDPAITDW 674
           PEYQ+TG++  +SDIY+FG++LLQL+TARS MGL++ +E+A+  + GKF +ILD    DW
Sbjct: 616 PEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDW 675

BLAST of HG10023463 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.6e-98
Identity = 278/790 (35.19%), Postives = 407/790 (51.52%), Query Frame = 0

Query: 21  IAISRDKNSQSAVKWAVEHLFNRKNAG-DCILIHVRSQSLH---------PQEADTVPKE 80
           +AIS    S++ +KWA+    + KN     I IH +  +L           +E + V   
Sbjct: 36  LAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEVAAA 95

Query: 81  CRPPTEAELHQFFL-PYRGFCARKGI-------------VAKEI-IIHDIDVPNALIDYV 140
            R     E  +  L P++  C RK +             VA E+ ++    V  A+   V
Sbjct: 96  YRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAITKEV 155

Query: 141 SNHSISNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVH--TKRLRKRH 200
           + H ISN+++G SS+ A  + +   D++  +  S+   C+VYV++ G VH   K      
Sbjct: 156 NQHLISNLIIGRSSQAASSRNY---DITASISASVSNLCTVYVVSNGGVHILAKDTSDTE 215

Query: 201 KSQTDARETRTRTPQIGRSLSKIQKSAYSN--------------------ISGLS---ED 260
           ++ T       RT     S S       SN                    + G+S   E 
Sbjct: 216 RNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPTIVRGVSVPMET 275

Query: 261 KIRDSSGT---SNDSMSGISDFSGPLSFKSIDTSFESTDF----SLTSSETSTRSFVSSN 320
              +S  T   S+D+    S  S P + +S+  + +  DF       SS +S   + +  
Sbjct: 276 SSTESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDERKDAMSSMSSNFEYGNVV 335

Query: 321 TP--------PSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRK 380
           TP            +E+ KLR EL+   EMY  A  E + A +K  EL+  ++ + E   
Sbjct: 336 TPLGHYFTDNQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNELKFEELTLLEHET 395

Query: 381 IERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEEKARIEAEERKK 440
              AK   E      +FE QK +   EAA           Q+++   E KA  EA+E++K
Sbjct: 396 KGIAKKETE------KFE-QKRREEREAA-----------QRRE--AEMKATHEAKEKEK 455

Query: 441 AME-LFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPD 500
             E       + Y+ ++ +EI  AT  F+E  KIG G YG VY+  L HT  A+K+L   
Sbjct: 456 LEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSA 515

Query: 501 RSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRLFRKDDTPPIP 560
            S   +QF QE+E+LS++RHPH+VLLLGACP++G LVYEYMENGSLEDRLF+ +D+ PIP
Sbjct: 516 ESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIP 575

Query: 561 WRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPS-- 620
           W  R RIA ++A+AL+FLH+ KP P++HRDLKPANILL+HN+VSK+GDVGL+ ++  +  
Sbjct: 576 WFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADP 635

Query: 621 VADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVE 680
           ++   T Y  T+  GT CYIDPEYQ+TG +  KSD+Y+FG+++LQL+T +  M L+Y VE
Sbjct: 636 LSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVE 695

Query: 681 EAIEHG---KFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPELVRLK 726
            A+E+    +  QILD    +WPIE+T  LA LAL+C ELR +DRPDL   +LP L  LK
Sbjct: 696 TAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLK 755

BLAST of HG10023463 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.3e-97
Identity = 195/438 (44.52%), Postives = 278/438 (63.47%), Query Frame = 0

Query: 243 ESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKE 302
           E  D   +  ETS +S         IE E+ +L+ EL+ T+  Y  A +E    + K K 
Sbjct: 291 EHRDSDRSPPETSRKS-----KKVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKM 350

Query: 303 LQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIIT 362
           L      + E +++  A   EE+ R  A  EK++   A +    A+ L   E  ++Q I 
Sbjct: 351 LS--TEYLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQ-IA 410

Query: 363 EEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLD 422
           E  A     E+KK ++    ++  YR+Y+I+EI  AT+ F+    IGEGGYG VYQ  LD
Sbjct: 411 EVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLD 470

Query: 423 HTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLED 482
            T  A+K++R D    +++F +E+EVLS++RHPH+VLLLGACPE GCLVYEY+ENGSLE+
Sbjct: 471 STPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEE 530

Query: 483 RLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGD 542
            +F + + PP+PW  RFR+  ++A  L FLH  KPEP+VHRDLKP NILL+ NYVSKI D
Sbjct: 531 YIFHRKNKPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIAD 590

Query: 543 VGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITAR 602
           VGLA+LV     D+VT Y  +  AGT  YIDPEY +TG +  KSD+Y+FG+++LQL+TAR
Sbjct: 591 VGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTAR 650

Query: 603 SPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLL 662
           +P G+   VE A++ G   ++LD ++TDWP+ +T  LA++ LKC E R RDRPDL++ ++
Sbjct: 651 NPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVI 710

Query: 663 PELVRLKNIGSGEMPPKG 681
           P L RL    + ++  +G
Sbjct: 711 PVLKRLVETANSKVKKEG 720

BLAST of HG10023463 vs. ExPASy TrEMBL
Match: A0A1S3CI92 (U-box domain-containing protein 35-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500712 PE=4 SV=1)

HSP 1 Score: 1264.6 bits (3271), Expect = 0.0e+00
Identity = 664/789 (84.16%), Postives = 703/789 (89.10%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEA 64
           MAEEETI+S+S +HT+IAISRDKNSQ AVKWAVEHLFNRKN GDCILIHVRSQ LHPQEA
Sbjct: 1   MAEEETIDSYSNNHTMIAISRDKNSQYAVKWAVEHLFNRKNTGDCILIHVRSQGLHPQEA 60

Query: 65  DTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVV 124
           D++PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID+VS HSISN+V
Sbjct: 61  DSIPKEYRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHVSKHSISNIV 120

Query: 125 VGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETRT 184
           VGAS+RN I++KFK+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  E++T
Sbjct: 121 VGASNRNPIMRKFKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMNESQT 180

Query: 185 RTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFES 244
            TP++G SLSK +KSAYS+ISGLSEDK RDSSGTSNDSMSGISDFSGPLSFKSI+TSFE+
Sbjct: 181 GTPRVGSSLSKTRKSAYSSISGLSEDKYRDSSGTSNDSMSGISDFSGPLSFKSIETSFEN 240

Query: 245 TDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQ 304
            DFS +SSETSTRS+VSS+TPPSIESEM++LR ELKQ MEMYDSASKEA VAKQKAKEL+
Sbjct: 241 LDFSPSSSETSTRSYVSSSTPPSIESEMKRLRFELKQMMEMYDSASKEAAVAKQKAKELE 300

Query: 305 QLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEE 364
           QLKME EED KIER KSA+E L ALAEFEKQKNKAA EA LVAQKLAELETQKKQIITEE
Sbjct: 301 QLKMEKEEDDKIERGKSADEALTALAEFEKQKNKAAAEATLVAQKLAELETQKKQIITEE 360

Query: 365 KARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHT 424
           KARIEAEERKK MELFERSNICYRRYSIDEIE ATDHFNESNKIGEGGYGPVYQALLDHT
Sbjct: 361 KARIEAEERKKTMELFERSNICYRRYSIDEIEVATDHFNESNKIGEGGYGPVYQALLDHT 420

Query: 425 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 484
           SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL
Sbjct: 421 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 480

Query: 485 FRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 544
           FRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG
Sbjct: 481 FRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 540

Query: 545 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 604
           LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP
Sbjct: 541 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 600

Query: 605 MGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPE 664
           MGLSYQVEEAIEH KFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL+PE
Sbjct: 601 MGLSYQVEEAIEHDKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLMPE 660

Query: 665 LVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI--KKKWS--------WN 724
           L RLKN+G GE PPK KL NV KA DQ   NSAPVLKGSESEI  +K+W         W+
Sbjct: 661 LSRLKNLGLGETPPKSKLANVSKAHDQQPPNSAPVLKGSESEIYVRKRWQRLQVSTRFWS 720

Query: 725 NSDAKVLNI-----------PLPISGKGVDVLSKKQ------QSSERCWSFVSCASAATS 767
                V  I            +PISGK VDV+ KKQ      QSS+RCWSF SCASAA  
Sbjct: 721 QKGIGVNTISSQIKKRWSIPSIPISGKRVDVMKKKQQQQQHLQSSQRCWSFASCASAANM 780

BLAST of HG10023463 vs. ExPASy TrEMBL
Match: A0A0A0KAY6 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450690 PE=4 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 649/791 (82.05%), Postives = 694/791 (87.74%), Query Frame = 0

Query: 1   MATPMAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLH 60
           MATPMAEEE+ +S+ST++TVIAISRDKNS  AVKWAVEHLFNRKN G+C+LIHVR+QSLH
Sbjct: 1   MATPMAEEESFDSYSTNNTVIAISRDKNSHYAVKWAVEHLFNRKNTGECVLIHVRTQSLH 60

Query: 61  PQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSI 120
           PQE DT+PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID++S HSI
Sbjct: 61  PQEVDTIPKEFRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHISKHSI 120

Query: 121 SNVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDAR 180
           SN+VVGAS+RN I++K K+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  
Sbjct: 121 SNIVVGASNRNPIMRKLKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMT 180

Query: 181 ETRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDT 240
           ET+T TP+I  SLSK QKSAYS+ISG SEDK R SSGTSNDS SGISDFSGPLSFKSI+T
Sbjct: 181 ETQTGTPRISGSLSKTQKSAYSSISGQSEDKYRYSSGTSNDSTSGISDFSGPLSFKSIET 240

Query: 241 SFESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKA 300
           SFE+ DFSL+SSETSTRS+VSS+TPP+IE EM+KLR ELK+ MEMYDSA KEA VAKQKA
Sbjct: 241 SFENQDFSLSSSETSTRSYVSSSTPPTIEYEMKKLRFELKKMMEMYDSACKEAAVAKQKA 300

Query: 301 KELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQI 360
           KEL+ LKME EED KIE  KS  E L  LAEFEKQKNKA  EA LVAQKLAELETQKK+I
Sbjct: 301 KELRHLKMEKEEDNKIECGKSTYEALTTLAEFEKQKNKAEAEATLVAQKLAELETQKKRI 360

Query: 361 ITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQAL 420
           ITEEKARIEAEERKK MELFERSNICYRR+SID+IE ATDHFNESNKIGEGGYGPVYQAL
Sbjct: 361 ITEEKARIEAEERKKTMELFERSNICYRRFSIDQIEVATDHFNESNKIGEGGYGPVYQAL 420

Query: 421 LDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSL 480
           L+HTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHP+MVLLLGACPEYGCLVYEYMENGSL
Sbjct: 421 LEHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPNMVLLLGACPEYGCLVYEYMENGSL 480

Query: 481 EDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI 540
           EDRLFRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI
Sbjct: 481 EDRLFRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKI 540

Query: 541 GDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 600
           GDVGLARLVPP+VADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT
Sbjct: 541 GDVGLARLVPPTVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLIT 600

Query: 601 ARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTV 660
           ARSPMGLSYQVEEAIEHGKFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDL TV
Sbjct: 601 ARSPMGLSYQVEEAIEHGKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLCTV 660

Query: 661 LLPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI--KKKWS------ 720
           L+PEL RLKN+GSGE PPK KL  V +A DQ   NSAPVLKGSE EI  +K+W       
Sbjct: 661 LMPELSRLKNLGSGETPPKSKLATVSRAHDQQVLNSAPVLKGSEGEIYVRKRWERLKVST 720

Query: 721 --WNNSDAKVLN-----------IP-LPISGKGVDVLSKKQQS---SERCWSFVSCASAA 767
             W+      LN           IP +PISGK VD   KKQQ    SERCWSF SCASAA
Sbjct: 721 RFWSQKGGIGLNTISSQIKKKWSIPSIPISGKRVDGSKKKQQPQQISERCWSFGSCASAA 780

BLAST of HG10023463 vs. ExPASy TrEMBL
Match: A0A1S3CGS8 (U-box domain-containing protein 35-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103500712 PE=4 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 627/702 (89.32%), Postives = 660/702 (94.02%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEA 64
           MAEEETI+S+S +HT+IAISRDKNSQ AVKWAVEHLFNRKN GDCILIHVRSQ LHPQEA
Sbjct: 1   MAEEETIDSYSNNHTMIAISRDKNSQYAVKWAVEHLFNRKNTGDCILIHVRSQGLHPQEA 60

Query: 65  DTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVV 124
           D++PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID+VS HSISN+V
Sbjct: 61  DSIPKEYRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHVSKHSISNIV 120

Query: 125 VGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETRT 184
           VGAS+RN I++KFK+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  E++T
Sbjct: 121 VGASNRNPIMRKFKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMNESQT 180

Query: 185 RTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFES 244
            TP++G SLSK +KSAYS+ISGLSEDK RDSSGTSNDSMSGISDFSGPLSFKSI+TSFE+
Sbjct: 181 GTPRVGSSLSKTRKSAYSSISGLSEDKYRDSSGTSNDSMSGISDFSGPLSFKSIETSFEN 240

Query: 245 TDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQ 304
            DFS +SSETSTRS+VSS+TPPSIESEM++LR ELKQ MEMYDSASKEA VAKQKAKEL+
Sbjct: 241 LDFSPSSSETSTRSYVSSSTPPSIESEMKRLRFELKQMMEMYDSASKEAAVAKQKAKELE 300

Query: 305 QLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEE 364
           QLKME EED KIER KSA+E L ALAEFEKQKNKAA EA LVAQKLAELETQKKQIITEE
Sbjct: 301 QLKMEKEEDDKIERGKSADEALTALAEFEKQKNKAAAEATLVAQKLAELETQKKQIITEE 360

Query: 365 KARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHT 424
           KARIEAEERKK MELFERSNICYRRYSIDEIE ATDHFNESNKIGEGGYGPVYQALLDHT
Sbjct: 361 KARIEAEERKKTMELFERSNICYRRYSIDEIEVATDHFNESNKIGEGGYGPVYQALLDHT 420

Query: 425 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 484
           SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL
Sbjct: 421 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 480

Query: 485 FRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 544
           FRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG
Sbjct: 481 FRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 540

Query: 545 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 604
           LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP
Sbjct: 541 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 600

Query: 605 MGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPE 664
           MGLSYQVEEAIEH KFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL+PE
Sbjct: 601 MGLSYQVEEAIEHDKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLMPE 660

Query: 665 LVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESE 707
           L RLKN+G GE PPK KL NV KA DQ   NSAPVLKGSESE
Sbjct: 661 LSRLKNLGLGETPPKSKLANVSKAHDQQPPNSAPVLKGSESE 702

BLAST of HG10023463 vs. ExPASy TrEMBL
Match: A0A5A7UXQ8 (U-box domain-containing protein 35-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001050 PE=4 SV=1)

HSP 1 Score: 1217.2 bits (3148), Expect = 0.0e+00
Identity = 626/703 (89.05%), Postives = 660/703 (93.88%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEA 64
           MAEEETI+S+S +HT+IAISRDKNSQ AVKWAVEHLFNRKN GDCILIHVRSQ LHPQEA
Sbjct: 1   MAEEETIDSYSNNHTMIAISRDKNSQYAVKWAVEHLFNRKNTGDCILIHVRSQGLHPQEA 60

Query: 65  DTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVV 124
           D++PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID+VS HSISN+V
Sbjct: 61  DSIPKEYRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHVSKHSISNIV 120

Query: 125 VGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETRT 184
           VGAS+RN I++KFK+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  E++T
Sbjct: 121 VGASNRNPIMRKFKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMNESQT 180

Query: 185 RTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFES 244
            TP++  SLSK +KSAYS+ISGLSEDK RDSSGTSNDSMSGISDFSGPLSFKSI+TSFE+
Sbjct: 181 GTPRVAGSLSKTRKSAYSSISGLSEDKYRDSSGTSNDSMSGISDFSGPLSFKSIETSFEN 240

Query: 245 TDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQ 304
            DFS +SSETSTRS+VSS+TPPSIESEM++LR ELKQ MEMYDSASKEA VAKQKAKEL+
Sbjct: 241 LDFSPSSSETSTRSYVSSSTPPSIESEMKRLRFELKQMMEMYDSASKEAAVAKQKAKELE 300

Query: 305 QLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEE 364
           QLKME EED KIER KSA+E L ALAEFEKQKNKAA EA LVAQKLAELETQKKQIITEE
Sbjct: 301 QLKMEKEEDDKIERGKSADEALTALAEFEKQKNKAAAEATLVAQKLAELETQKKQIITEE 360

Query: 365 KARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHT 424
           KARIEAEERKK MELFERSNICYRRYSIDEIE ATDHFNESNKIGEGGYGPVYQALLDHT
Sbjct: 361 KARIEAEERKKTMELFERSNICYRRYSIDEIEVATDHFNESNKIGEGGYGPVYQALLDHT 420

Query: 425 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 484
           SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL
Sbjct: 421 SVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRL 480

Query: 485 FRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 544
           FRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG
Sbjct: 481 FRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVG 540

Query: 545 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 604
           LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP
Sbjct: 541 LARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSP 600

Query: 605 MGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPE 664
           MGLSYQVEEAIEH KFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL+PE
Sbjct: 601 MGLSYQVEEAIEHDKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLMPE 660

Query: 665 LVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI 708
           L RLKN+G GE PPK KL NV KA DQ   NSAPVLKGSESE+
Sbjct: 661 LSRLKNLGLGETPPKSKLANVSKAHDQQPPNSAPVLKGSESEV 703

BLAST of HG10023463 vs. ExPASy TrEMBL
Match: A0A1S4E3M2 (U-box domain-containing protein 35-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103500712 PE=4 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 614/732 (83.88%), Postives = 650/732 (88.80%), Query Frame = 0

Query: 62  QEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSIS 121
           +EAD++PKE RPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALID+VS HSIS
Sbjct: 33  EEADSIPKEYRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDHVSKHSIS 92

Query: 122 NVVVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARE 181
           N+VVGAS+RN I++KFK+PDV+TCLLKS PESCSVYVIARGKVHTKRLRKR+KSQTD  E
Sbjct: 93  NIVVGASNRNPIMRKFKNPDVATCLLKSAPESCSVYVIARGKVHTKRLRKRNKSQTDMNE 152

Query: 182 TRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTS 241
           ++T TP++G SLSK +KSAYS+ISGLSEDK RDSSGTSNDSMSGISDFSGPLSFKSI+TS
Sbjct: 153 SQTGTPRVGSSLSKTRKSAYSSISGLSEDKYRDSSGTSNDSMSGISDFSGPLSFKSIETS 212

Query: 242 FESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAK 301
           FE+ DFS +SSETSTRS+VSS+TPPSIESEM++LR ELKQ MEMYDSASKEA VAKQKAK
Sbjct: 213 FENLDFSPSSSETSTRSYVSSSTPPSIESEMKRLRFELKQMMEMYDSASKEAAVAKQKAK 272

Query: 302 ELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQII 361
           EL+QLKME EED KIER KSA+E L ALAEFEKQKNKAA EA LVAQKLAELETQKKQII
Sbjct: 273 ELEQLKMEKEEDDKIERGKSADEALTALAEFEKQKNKAAAEATLVAQKLAELETQKKQII 332

Query: 362 TEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALL 421
           TEEKARIEAEERKK MELFERSNICYRRYSIDEIE ATDHFNESNKIGEGGYGPVYQALL
Sbjct: 333 TEEKARIEAEERKKTMELFERSNICYRRYSIDEIEVATDHFNESNKIGEGGYGPVYQALL 392

Query: 422 DHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLE 481
           DHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLE
Sbjct: 393 DHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLE 452

Query: 482 DRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIG 541
           DRLFRKD+TPPIPWR RFRIA DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIG
Sbjct: 453 DRLFRKDNTPPIPWRIRFRIACDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIG 512

Query: 542 DVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITA 601
           DVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITA
Sbjct: 513 DVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITA 572

Query: 602 RSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL 661
           RSPMGLSYQVEEAIEH KFP+ILDP ITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL
Sbjct: 573 RSPMGLSYQVEEAIEHDKFPEILDPTITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVL 632

Query: 662 LPELVRLKNIGSGEMPPKGKLPNVGKAPDQLASNSAPVLKGSESEI--KKKWS------- 721
           +PEL RLKN+G GE PPK KL NV KA DQ   NSAPVLKGSESEI  +K+W        
Sbjct: 633 MPELSRLKNLGLGETPPKSKLANVSKAHDQQPPNSAPVLKGSESEIYVRKRWQRLQVSTR 692

Query: 722 -WNNSDAKVLNI-----------PLPISGKGVDVLSKKQ------QSSERCWSFVSCASA 767
            W+     V  I            +PISGK VDV+ KKQ      QSS+RCWSF SCASA
Sbjct: 693 FWSQKGIGVNTISSQIKKRWSIPSIPISGKRVDVMKKKQQQQQHLQSSQRCWSFASCASA 752

BLAST of HG10023463 vs. TAIR 10
Match: AT5G12000.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 643.3 bits (1658), Expect = 2.5e-184
Identity = 373/720 (51.81%), Postives = 489/720 (67.92%), Query Frame = 0

Query: 1   MATPMAEEETINSHS-TSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVR-SQS 60
           MA P   ++   SH+  + TV+AI +DKNS  AV+WAV+HLFN       IL+HVR   S
Sbjct: 1   MARPHPSDD---SHTLVNSTVVAIDKDKNSHFAVRWAVDHLFNMIINSKMILLHVRLKNS 60

Query: 61  LHPQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNH 120
            H    D          ++EL+Q F+PYRG+CARKGI   E+I+ D DV  A++DYV+N+
Sbjct: 61  NHGGNID----------DSELNQLFVPYRGYCARKGISMMEVILDDTDVSKAVLDYVNNN 120

Query: 121 SISNVVVGASSRNAI---LKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKR--- 180
            ++N+V+G+SS++     LK  KS DV++ +LKS PE CSVYVI++GKVH+ R  +R   
Sbjct: 121 LVTNLVLGSSSKSPFARSLKFTKSHDVASSVLKSTPEFCSVYVISKGKVHSSRTAQRPIT 180

Query: 181 --------------------HKSQTDARETRTRTPQ----------IGRSLSKIQKSA-- 240
                                +S    R  R   P+            R + KI  +   
Sbjct: 181 NTLVPPRVPSSTFHLPDPDHDRSPRSQRNGRNTVPERYSHENKGFKPVREMHKIPTNGSL 240

Query: 241 ---YSNISGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFESTDFSLTSSETSTR 300
              Y    G  +   R+S+G S+ S    SD    +   SID S E+ D  +  S +S  
Sbjct: 241 DFNYEFRQGKGQ---RNSTGRSSFSDES-SDVGSMMMMGSIDLSAENFDM-VGGSGSSDE 300

Query: 301 SFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRKIE 360
           S  +S +   IE+EM++L++ELKQTM+MY SA KEA+ AK+KA EL Q K  MEE R+ E
Sbjct: 301 S--ASQSTRDIEAEMKRLKIELKQTMDMYSSACKEALNAKKKANELNQWK--MEEARRFE 360

Query: 361 RAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEEKARIEAEERKKAM 420
            A++AEE   A+AE EK K +AA EAA  AQ++AELE Q+++   E KAR E++E+ +A+
Sbjct: 361 EARNAEEAALAVAEMEKAKCRAALEAAEKAQRMAELEGQRRK-QAEMKARRESQEKDRAL 420

Query: 421 ELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPDRSH 480
               ++++ YR+YSIDEIE AT+ F  + KIGEGGYGPVY   LDHT VAIK+LRPD + 
Sbjct: 421 SALVQNDVRYRKYSIDEIEVATERFANNRKIGEGGYGPVYHGTLDHTPVAIKVLRPDAAQ 480

Query: 481 GQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRLFRKDDTPPIPWRT 540
           G++QFQQE+EVLS +RHPHMVLLLGACPEYGCLVYE+M+NGSLEDRLFR+ ++PP+ WR 
Sbjct: 481 GKKQFQQEVEVLSSIRHPHMVLLLGACPEYGCLVYEFMDNGSLEDRLFRRGNSPPLSWRK 540

Query: 541 RFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPSVADSV 600
           RF+IA++IATAL FLHQ KPEP+VHRDLKPANILLD NYVSKI DVGLARLVP SVA++V
Sbjct: 541 RFQIAAEIATALSFLHQAKPEPLVHRDLKPANILLDKNYVSKISDVGLARLVPASVANTV 600

Query: 601 TQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVEEAIEH 660
           TQYHMT+AAGTFCYIDPEYQQTG L  KSDI+S G++LLQ+ITA+SPMGL++ V  AI+ 
Sbjct: 601 TQYHMTSAAGTFCYIDPEYQQTGKLTTKSDIFSLGIMLLQIITAKSPMGLAHHVSRAIDK 660

Query: 661 GKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPELVRLKNIGSGEMP 678
           G F  +LDP + DWP+E+ L  A+L L+C ELRKRDRPDL   ++PEL+RL+N+G    P
Sbjct: 661 GTFKDMLDPVVPDWPVEEALNFAKLCLRCAELRKRDRPDLGKEIVPELLRLRNLGKDNEP 697

BLAST of HG10023463 vs. TAIR 10
Match: AT1G17540.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 619.0 bits (1595), Expect = 5.1e-177
Identity = 356/710 (50.14%), Postives = 484/710 (68.17%), Query Frame = 0

Query: 5   MAEEETINSHSTSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEA 64
           MA        S + T IAI +DKNSQ A+KWAVE++    ++ +CIL+HV+++       
Sbjct: 1   MAARRLKGKDSKAVTAIAIDKDKNSQHALKWAVENII--IDSPNCILLHVQTKLRFGAGE 60

Query: 65  DTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNVV 124
           +T   E     + E HQFFLP+RGFCARKGI+A E+++HDID+ +A++DY++N+SISN+V
Sbjct: 61  NT---EAPHDNQEEAHQFFLPFRGFCARKGIIATEVLLHDIDISSAIVDYITNNSISNIV 120

Query: 125 VGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKR------------ 184
           +GAS+RN+ LKKFKS DV T LLK+ P++C+V+++++GK+ T R   R            
Sbjct: 121 IGASARNSFLKKFKSVDVPTTLLKTTPDTCAVFIVSKGKLLTSRSASRPQTPQGPHTPQG 180

Query: 185 -----------HKSQ-----TDARETRTRTPQIGRSLSKI-----------QKSAY-SNI 244
                      H S+     +D   T + + + GRS   +           + S Y S++
Sbjct: 181 PQTPQAPQHPPHPSKHPSMMSDPGPTSSTSSESGRSSPALNGELSPPTPHYKPSLYRSSM 240

Query: 245 SGLSEDKIRDSSGTSNDSMSGISDFSGPLSFKSIDTSFESTDFSLTSSETS-TRSFVSSN 304
           S LS +   D S  SN S   I      L   +   S  S+   +T  E S +   ++ +
Sbjct: 241 SELSNEFPSDHSAESNASFYSI------LGRSTYGGSSHSSMSEITDGEESLSGGNITEH 300

Query: 305 TPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRKIERAKSAE 364
              ++E+E+R+LR EL+Q           A ++++ A  L   +   E +R +E AK+A 
Sbjct: 301 QNQNLEAEVRRLRFELQQF---------NASMSRESAPHLLGPRATAETER-LEEAKAAR 360

Query: 365 EVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEEKARIEAEERKKAMELFERS 424
           E+LRAL+E +KQK + A +A  VAQ+LAE+ETQK++++ E +AR + +            
Sbjct: 361 EMLRALSEMDKQKTQTAIQATEVAQRLAEIETQKRRLV-EMQARFKEQNM--------AD 420

Query: 425 NICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPDRSHGQRQFQ 484
           +I YRRYSI ++E ATD F+++ KIGEGGYGPVY+A+L++TSVAIK+L+ D S G +QF 
Sbjct: 421 SISYRRYSIRDVEGATDGFSDALKIGEGGYGPVYKAVLENTSVAIKLLKSDVSQGLKQFN 480

Query: 485 QEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRLFRKDDTPPIPWRTRFRIAS 544
           QEIEVLS MRHP+MV+LLGACPEYGCLVYEYMENG+LEDRLF KD+TPP+ WR RFRIA+
Sbjct: 481 QEIEVLSCMRHPNMVILLGACPEYGCLVYEYMENGTLEDRLFCKDNTPPLSWRARFRIAA 540

Query: 545 DIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPSVADSVTQYHMT 604
           +IAT LLFLHQ KPEP+VHRDLKPANIL+D ++ SKI DVGLARLVP +VADS + YHMT
Sbjct: 541 EIATGLLFLHQAKPEPLVHRDLKPANILIDRHFTSKISDVGLARLVPAAVADSFSNYHMT 600

Query: 605 AAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVEEAIEHGKFPQI 664
           AAAGTFCYIDPEYQQTGMLGVKSD+YSFGV+LLQ+ITA   MGLS++VE+AIE  K  ++
Sbjct: 601 AAAGTFCYIDPEYQQTGMLGVKSDLYSFGVVLLQIITAMPAMGLSHRVEKAIEKKKLREV 660

Query: 665 LDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPELVRLKNIGS 674
           LDP I+DWP E+T+ LAQLAL+CCELRK+DRPDL +VLLP L +L+   +
Sbjct: 661 LDPKISDWPEEETMVLAQLALQCCELRKKDRPDLASVLLPALSKLREFAT 680

BLAST of HG10023463 vs. TAIR 10
Match: AT5G26150.1 (protein kinase family protein )

HSP 1 Score: 605.5 bits (1560), Expect = 5.8e-173
Identity = 355/716 (49.58%), Postives = 476/716 (66.48%), Query Frame = 0

Query: 1   MATPMAEEETINSHS-TSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQ-S 60
           MA+P + ++    HS  + TV+AI ++K+S  AV+WAV+HL N  +    IL+HVR++ S
Sbjct: 1   MASPYSSDDI---HSPVNSTVVAIDKEKHSSYAVRWAVDHLLNMIHNPVMILVHVRTKNS 60

Query: 61  LHPQEADTVPKECRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNH 120
            H    +            +L+Q F+PYRG+CARKG+V     + D DV   ++DYV+N+
Sbjct: 61  NHGANLN----------NDDLNQLFIPYRGYCARKGVV-----LDDSDVAKTILDYVNNN 120

Query: 121 SISNVVVGASSRNAILKKF---KSPDVSTCLLKSIPESCSVYVIAR-GKVHTKRLRKRHK 180
            ++N+V+GAS++N   + F   K  +V + ++KS P+ CSVYVI++ GKV + R  +R  
Sbjct: 121 LVNNLVLGASTKNTFARSFMFSKPHEVQSSIMKSTPDFCSVYVISKGGKVQSSRPAQRPI 180

Query: 181 SQTDARETRTRTPQIGRSLS--------KIQKSAYSNISGLSEDKIRDSSGTS----NDS 240
           + T A      +  + +SLS        ++Q+SA +  +  +    R +  T+       
Sbjct: 181 TNTLAPPRVPSSGFLIQSLSDSEQDLIPRVQRSARNKPNETTYPHNRAAFNTTQKGYKSP 240

Query: 241 MSGISDFSGPLS------------------------FKSIDTSFE-STDFSLTSSETSTR 300
           ++G  DF+   +                          S+D S + S DF   +S +S  
Sbjct: 241 INGSMDFNNGFNQAAFQRNPTLQSSFSDESDGGFGVMGSVDLSSQNSMDFYHGASSSSEE 300

Query: 301 SFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEAVVAKQKAKELQQLKMEMEEDRKIE 360
           S   S     IE+EMR+L+LELKQTM+MY SA KEA+ AK+KA EL Q K  +EE RK E
Sbjct: 301 SIPQST--KDIEAEMRRLKLELKQTMDMYSSACKEALTAKRKANELNQWK--IEEARKFE 360

Query: 361 RAKSAEEVLRALAEFEKQKNKAATEAALVAQKLAELETQKKQIITEEKARIEAEERKKAM 420
           +A+ +EE   A+AE EK K + A EAA  AQ++AELE Q+++   E KA  E +++ +A+
Sbjct: 361 KARLSEEAALAVAEIEKAKCRTAVEAAEKAQRMAELEGQRRK-QAEMKAVSEEKDKDRAV 420

Query: 421 ELFERSNICYRRYSIDEIEAATDHFNESNKIGEGGYGPVYQALLDHTSVAIKILRPDRSH 480
                +++ YR+YSI+EIE AT+ F    KIGEGGYGPVY   LDHT VAIK+LRPD + 
Sbjct: 421 SALAHNDVRYRKYSIEEIEEATERFANHRKIGEGGYGPVYNGELDHTPVAIKVLRPDAAQ 480

Query: 481 GQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVYEYMENGSLEDRLFRKDDTPPIPWRT 540
           G++QFQQE+EVL  +RHPHMVLLLGACPEYGCLVYE+MENGSLEDRLFR  ++PP+ WR 
Sbjct: 481 GKKQFQQEVEVLCSIRHPHMVLLLGACPEYGCLVYEFMENGSLEDRLFRTGNSPPLSWRK 540

Query: 541 RFRIASDIATALLFLHQMKPEPVVHRDLKPANILLDHNYVSKIGDVGLARLVPPSVADSV 600
           RF IA++IATAL FLHQ KPEP+VHRDLKPANILLD NYVSKI DVGLARLVP S+ADSV
Sbjct: 541 RFEIAAEIATALSFLHQAKPEPLVHRDLKPANILLDKNYVSKISDVGLARLVPASIADSV 600

Query: 601 TQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFGVLLLQLITARSPMGLSYQVEEAIEH 660
           TQ+HMT+AAGTFCYIDPEYQQTGML  KSD+YS G+LLLQ+IT R PMGL++QV  AI  
Sbjct: 601 TQFHMTSAAGTFCYIDPEYQQTGMLTTKSDVYSLGILLLQIITGRPPMGLAHQVSRAISK 660

Query: 661 GKFPQILDPAITDWPIEDTLGLAQLALKCCELRKRDRPDLRTVLLPELVRLKNIGS 674
           G F ++LDP + DWP+++    A LALKC ELRKRDRPDL   ++P L+RLKN G+
Sbjct: 661 GTFKEMLDPVVPDWPVQEAQSFATLALKCAELRKRDRPDLGKEVVPHLIRLKNFGN 693

BLAST of HG10023463 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 599.4 bits (1544), Expect = 4.2e-171
Identity = 357/740 (48.24%), Postives = 470/740 (63.51%), Query Frame = 0

Query: 15  STSHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVR--------SQSLHPQEADT 74
           S+    +AI +DK+SQ A+KWAV++L  R  +   IL+HV+        S SLH   A  
Sbjct: 16  SSGVVAVAIDKDKSSQHALKWAVDNLLQRGQS--VILVHVKLRPSPLNNSASLHASSAKL 75

Query: 75  VPKE---CRPPTEAELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYVSNHSISNV 134
                  CR P E    + FLP+R FC RK I  +++++ + DV  AL++YV+  +I  +
Sbjct: 76  SQDSSLVCRDP-EGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKALVEYVNQAAIEVL 135

Query: 135 VVGASSRNAILKKFKSPDVSTCLLKSIPESCSVYVIARGKVHTKRLRKRHKSQTDARETR 194
           VVG+SS+   L+  K  D+   + K+ P+ C+VY+I++GK+ T R   R    T    + 
Sbjct: 136 VVGSSSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSASRSAPMTAPLRSS 195

Query: 195 TRTPQI----------GRSLSKIQKSAYSNISGLSEDKIR----DSSGTSNDSMS----- 254
            + P +            SL   ++S  SN     ED+ R    D    S D  S     
Sbjct: 196 VQPPSLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQRRSIDDQSESFRS 255

Query: 255 ---------GISDFSGP---LSF-----KSID-------------------TSFESTDFS 314
                       D + P   +SF      SID                   ++F   D+S
Sbjct: 256 PFTRRGNGRSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDPSRTPPRLSNFSDMDYS 315

Query: 315 LTSSETST--RSFVSSNTP---------------PSIESEMRKLRLELKQTMEMYDSASK 374
            +  ++S   R  V  N+P                 +E+EMR+L+LELKQTMEMY +A K
Sbjct: 316 SSLDQSSNYGRRSVDMNSPTDFESERFSSASQSIDDVEAEMRRLKLELKQTMEMYSTACK 375

Query: 375 EAVVAKQKAKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNKAATEAALVAQKLA 434
           EA+ AKQKA ELQ+ K  +EE+RK+E A++AEE   A+AE EK K+KAA EAA  AQ++A
Sbjct: 376 EALTAKQKATELQRWK--LEEERKLEEARNAEEAALAIAEKEKAKSKAAMEAAEAAQRIA 435

Query: 435 ELETQKKQIITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEG 494
           ELE  KK++  E KA  E+EE+ KA+     S++ YR+YSI++IE AT+ F E  KIGEG
Sbjct: 436 ELEA-KKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIEDIELATEFFAEKYKIGEG 495

Query: 495 GYGPVYQALLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLV 554
           GYGPVY+  LDHT VA+K+LRPD + G+ QFQQE+EVLS +RHP+MVLLLGACPE GCLV
Sbjct: 496 GYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPECGCLV 555

Query: 555 YEYMENGSLEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANIL 614
           YE+M NGSLEDRLFR  ++PP+ W+ RFRIA++I T LLFLHQ KPEP+VHRDLKP NIL
Sbjct: 556 YEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLFLHQAKPEPLVHRDLKPGNIL 615

Query: 615 LDHNYVSKIGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSF 672
           LD N+VSKI DVGLARLVPP+VAD+VTQY MT+ AGTFCYIDPEYQQTGMLGVKSDIYS 
Sbjct: 616 LDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSL 675

BLAST of HG10023463 vs. TAIR 10
Match: AT1G72760.1 (Protein kinase superfamily protein )

HSP 1 Score: 584.3 bits (1505), Expect = 1.4e-166
Identity = 340/681 (49.93%), Postives = 461/681 (67.69%), Query Frame = 0

Query: 17  SHTVIAISRDKNSQSAVKWAVEHLFNRKNAGDCILIHVRSQSLHPQEADTVPKECRPPTE 76
           S T IAI +DKNSQ A+KWAVE++    +A  C+LIHV       Q  DT          
Sbjct: 15  SITAIAIDKDKNSQHALKWAVENIV--ADAPQCVLIHV-------QLGDT-GGHFHQDNP 74

Query: 77  AELHQFFLPYRGFCARKGIVAKEIIIHDIDVPNALIDYV-SNHSISNVVVGASSRNAILK 136
            E H+FFLP+RGFCARKGI+AKE+I+HDID+ NA+++Y+ +N+ I+N+VVGAS+RN+ LK
Sbjct: 75  DEAHEFFLPFRGFCARKGIIAKEVILHDIDISNAIVNYITNNYYIANLVVGASARNSFLK 134

Query: 137 KFKSPDVSTCLLKSIPESCSVYVIARGK-VHTKRLRKRHK-------------------- 196
           KF+SPDV T LLK+ PE+C+V+V+ +GK + ++    RHK                    
Sbjct: 135 KFQSPDVPTTLLKTTPETCAVFVVTKGKLLKSRSASHRHKLSRQQNLSSLLYNSTNSIDS 194

Query: 197 -SQTDARETRTRTPQIGRSLSKIQKSAYSNISGLSEDKIRDSSGTSNDSMSGISDFSGPL 256
            S + +    T+T +   +  +      S    +SE    ++   S D +S +S ++   
Sbjct: 195 DSASISSPVSTQTNKPNSNFFQPNSPRISTPQSMSEISQSETDNGSCDMVSTVSSYTVSD 254

Query: 257 SFKSIDTSFESTDFSLTSSETSTRSFVSSNTPPSIESEMRKLRLELKQTMEMYDSASKEA 316
           S  +I +S  S     TS+E+            ++E+E+R+LRLELKQ      +  K+ 
Sbjct: 255 SSTTIGSSISS-----TSTESPLVGNYVEQQNQNLEAEVRRLRLELKQF-----NKDKDT 314

Query: 317 VVAKQKAKELQQLKMEMEEDRKIERAKSAEEVLRALAEFEKQKNK-AATEAALVAQKLAE 376
              K+ ++E          D KI       E+ RAL++ E QK + AA +AA +A+++A+
Sbjct: 315 TNQKENSQETPW------SDEKI-------ELPRALSDRETQKTQSAAFQAAEIAKRIAK 374

Query: 377 LETQKKQIITEEKARIEAEERKKAMELFERSNICYRRYSIDEIEAATDHFNESNKIGEGG 436
           +E+QK++++      ++A   K+ M     + + YRRYSI ++E AT  F+++ KIGEGG
Sbjct: 375 MESQKRRLL-----EMQANLDKQMM----FTTVSYRRYSIKDVEDATYGFSDALKIGEGG 434

Query: 437 YGPVYQALLDHTSVAIKILRPDRSHGQRQFQQEIEVLSRMRHPHMVLLLGACPEYGCLVY 496
           YGPVY+A+LD+TSVAIKIL+   + G +QFQQEIEVLS MRHP+MV+LLGACPEYGCLVY
Sbjct: 435 YGPVYKAVLDYTSVAIKILKSGITEGLKQFQQEIEVLSSMRHPNMVILLGACPEYGCLVY 494

Query: 497 EYMENGSLEDRLFRKDDTPPIPWRTRFRIASDIATALLFLHQMKPEPVVHRDLKPANILL 556
           EYMENG+LEDRLF K++TPP+ WR RFRIAS+IAT LLFLHQ KPEP+VHRDLKPANILL
Sbjct: 495 EYMENGTLEDRLFCKNNTPPLSWRARFRIASEIATGLLFLHQAKPEPLVHRDLKPANILL 554

Query: 557 DHNYVSKIGDVGLARLVPPSVADSVTQYHMTAAAGTFCYIDPEYQQTGMLGVKSDIYSFG 616
           D +   KI DVGLARLVPP+VAD+ + YHMT+AAGTFCYIDPEYQQTGMLGVKSD+YSFG
Sbjct: 555 DKHLTCKISDVGLARLVPPAVADTYSNYHMTSAAGTFCYIDPEYQQTGMLGVKSDLYSFG 614

Query: 617 VLLLQLITARSPMGLSYQVEEAIEHGKFPQILDPAITDWPIEDTLGLAQLALKCCELRKR 674
           V+LLQ+ITA+  MGL ++VE A+E+    +ILDP +++WP E+TL LA+LAL+CCELRK+
Sbjct: 615 VVLLQIITAQPAMGLGHKVEMAVENNNLREILDPTVSEWPEEETLELAKLALQCCELRKK 653