HG10023444 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10023444
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr05: 34289059 .. 34293751 (+)
RNA-Seq Expression	HG10023444
Synteny	HG10023444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACATCGGAGCAAGCCTTCGTAACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTTCTCAAACTCACTGCTTCCTGTTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTAAGAGTTTCTGATCTTCGTCATATCATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGCGGCACCGTGGAGGCCAGGTCTTCTGTGGAAGGTAATTGGAAGAGCTTACAGCCTAATGTATTGATTTCTCAAGAATCCGGAGATAGAGCGGTGATTATTCAGAGGACTCAAGTGGTAACTCCTTTGATATTTCTGTTAAGACTCTGCGCGACTGTGTTAAGTTTTTGTTTTGTTTTTTGGGTTAGCTGAGGTATGCAGTTTAGCCGTTTGTTAGTACGTTATGCTTGCAGAGTATTGTGACAGTATTCGAGTAATTTGATAGCTCCATTAAAATTGTAAACAAAGTCTAATCAATTAAACAACTAACTTATGCATCTTTTAGATTTTTCACATTTTTCACATTACAAGGTCTCTAGTCTAGGTACTCGGTTCAAGAGGTAGTTATGATACGATTCTATCCTGGATGAACCACGAAAGAGAAGGATCACCGGGTGAAATAGCTGTTGAATTTATCTCTATAGGTCTTCTGGCTTGCCTGAAGTTGATCTGTCATCGTGTGTGCCTGTGTGTATAGAACTACGATGTGGGGATTAAGTATTTGGGAAATCTTGAACTAATTAATCAACATAATTTCTTTATGAATTTAATTACAGGGAAAATGAATCTCTCTTTTTTCAGGATTAACGGTCGTAATCTCACTGGTTCTGCAAGTGTTTGTTTTTGTTCCCCTTGGTTATTCAGTGTGATGGAGATGTGTATATACTAATCCAGTTCTATAAACTAAAGCTTTTTTGTTGTATCATGGCATTGTTTAGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCCGACTTACGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTAAAGTCAAGGCTTATATTTGTTATTGTTATGATTGATTCACTGTTTTCATTACCCTTACCAAAAAAAGATCTCTGTATTAAATACCTTTATATTTAGAATGATATGGGACTTTTTTATTCTTTCTGTGAATAAAGAACTAACTTATTGAAATTGGGGCCATAAGTAGGTTTGTAAGTGCTACCTCTTAAGAAATGAGTCCAACAGAGCAGGCTTGAAGACATACCCTTAGTGTCAAGAAATAAGGATTTTATGGTTGCTTACCTATTATATATAAAAGCTAAATCTTCATAATATGAGATTGATGTGTGGCCGTTGTGATTTGTGAAGGTAAAATATATAGTTTAGATGTCTGGTTGAGCAGCAGGAAACTATATTGCAGCGGTCATTGTACAAGAATTCAGTGCAGTTTGCATATGAAGTCTTATGTGATGCTGATATTTGAGAAAATTGTAACAGGTTCCTTTTGTTCTTGTTGAGGGTCATTCACCATACTCTGATTTTGTTGTCGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCATGAGTACCCTGTGAGTAAGGCAGTATATATTGAAGTCGAACACCACTTTTTTTATAAATAGATTTTAGCTAAAAATTCTTTGTTATGTTCTACTTTTGAAGGTTGGGGTGCTGGATGAGGAGAAGATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGAGTTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTGTAAGTTTATACATTTCAAAGTAGATCATAGATGCATATATGGATATAAAATTGATAATAAAGGACCCGTTTGATAACATTCTCGTTTCTTGTTTCTGTTTCTCATTTTTCAAAAAATGGATTTGTTTGATAACCGTTCTCATTTCTTGTTTAGTTCCTATTGTTACGGTTTCTCTCTATACTTTTTCGTTCCACAAATTAACCAAACTTGGTCTTTGCTCTGCTGGTATTAGGCAGTGAAGGAGTCCAATTTTAATCTACCAGTCATGGGTCCAAATCCCATAAATAGCAATTTTGGGACCTTTTCGTTTTTAATTTTTATTTATTGAATAATTTGATTTTTATATTATATTTCAATATCCCATCCAAATATTTCTTAATTTTCCTTTATTCATGTATTAGTTTAATATTGAATTACAATTTTTCTAATATTTAGATTTATATATTATTTTAATTTTTCAATAGGTAAATCTCAATATCTTTTTAATTTTAAATTTTGCAGAGTAGTATAGTTGAGTTAGATTTTTTTTTTTTGCTATCCGTGAGTGTCCGGGCCAGCTTACGCGCACCTCGACCAATCTCACGGGACAATCTACCTGACCCTACAACATTTGGGTATCAAGGAAACTCGTAGGAAATTTTTTCCTAGGTAGGTGGCCACCATAGATTGAACCCATGACCTCTTAGCCATATAGTTGAGTTAGATGTGGGTCGAAATTATTTAAGACAATGACTTGAGTGAGAGATGATATTGCTGATCAAATTTGGGCAACATTTGAAGGATAGATACAATTCATTTTTATATTTAGTTGTGCATTGAAACTTATTTGTGTTAGACAAGAATTAAAATATCGATATCGACATCGACATTTCAATTTTACGGATATATCGACAAAATATCGATATCAATAGATATTTTTGTAAATCATGAATTTTATAAAAATTATTTAGATTAATAATTAAGTTGTTTTTACTCCAAAACTAAGTTATGGATATTATTGTTATTAGTATTCATGTTCATATTGGATTAAATAGATGACATTTTTTTTTATGTTTTCTAAGTATTAATAAAAGATGTTGAAGATATCCATGGATATTTATTGTCGATATAAAACCCTTCGATTTACGGATATATCGATGGTTAGTTTCGGCCTTTATTAGATGATAAAAGCTTTTGATAATTTCTTTTTCGATATTTAATATGTGACAATTTCTTTTTTATATTATATTACTACAAAAAAAACAAGAAACAAAAATAAAAAATAGTTATCAAATAAGTTTCTGTTTCTTTTTTTCAAGAAACAGGAAATATGAATAGTTATCAAACATGTTTCTGTTTCTAGTTTTTAAAATACAGGAAACAAGAAACAAGAAATGGGAAACATGAACGTTATCAAACGGGCCCAAATGTGTCTGTCTGCCAATGGTGAACCCCGTTGATTGGGTTTGGGGCTCTGGATGGGACTTACTAGGCCATAGGATTAAGCCTCCGAGTAAAAAGCTCTTGTGGTCTTTTGGAGCTAAATAGATTTGTGTGTATTTGTTGTCTGGGTCCCAATACTCTCCACTTTCTATATGTGTGTGTGCGCGCTCTCATCTCCAGTTGAAGTGTGGATTTTGGTTTGTACCTAAATTAGTCAACACATCTTGTCTCGTCAGTGGTTCTAACTAATGGCTAGTCTCTCCTCTCCCACACCCACCGTGAGGACAAAAAAAAAAAAAAAAACCGTGCCTGATGTGACATGCAGTGCAATTTCTTCTTTGTTGAATATGGGGAATAATGTCTAATTGGACCAAAAAAAAAAAATTGTTTCCTTAATGTTTTCCTTGGTTTCTTTGTATTTGGTATCTTATATTTTATTTTGAGCATTAATCTCTTTTCATCTTTACAGAGAAAGTGATTCATTTGAAAAACTGTCTAATTGGACAAAATTTATTACTGTTTGCACTTCGAGATAATCTTCATTGGAATAATGGATATTATGATGATGTACTCTTCTTTGCAGATGTGAAATGACTAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAGATTTCTATTCTGGAGTAGTATTGTCCTTGGTTCATTGTCTGTTGGCATTCTTGGTTATTCTGTTGTGAGGTATGTTATCTTCCTTAATTTCTTCTTTTATTTTATTTTATTTTGCGAGGTTTCTTTGTGTTGATATTTTTCTTTAGGCAAAGCAATGCCTTGGCTTAATATGCCTTCCATGAATTCTTTATTCTGCTTTGCACTATGCTTTCTGAATACCTTCCTTTAGTCCTTTTATTAGGCTGTTCCCAAGTATACTCGACTTGTCTGGTTGCTTCGTTCTTTCTATGATCGCATTTTAAAAGTTTAATGAGTATCAGTGTAATATCCATTCTCTTCAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACGGTTACAGAATTCAAGGAATGATTCCACCACATCACAACTGGATTCAAGCAACGACATTCCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGTTAGCAGCCATTCCAGACGACGAGTTAGCAGCAAATGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGATGTGCCGTGTCAGTTGAACGCGATTCAGCTCCCAAATGTCCTGTCTGCCGCCAGCAAATCCGAAGTTCTGTGCGGATTTACTATTCATGA

mRNA sequence

ATGTCCACATCGGAGCAAGCCTTCGTAACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTTCTCAAACTCACTGCTTCCTGTTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTAAGAGTTTCTGATCTTCGTCATATCATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGCGGCACCGTGGAGGCCAGGTCTTCTGTGGAAGGTAATTGGAAGAGCTTACAGCCTAATGTATTGATTTCTCAAGAATCCGGAGATAGAGCGGTGATTATTCAGAGGACTCAAGTGGTTCCTTTTGTTCTTGTTGAGGGTCATTCACCATACTCTGATTTTGTTGTCGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCATGAGTACCCTGTTGGGGTGCTGGATGAGGAGAAGATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGAGTTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTATGTGAAATGACTAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAGATTTCTATTCTGGAGTAGTATTGTCCTTGGTTCATTGTCTGTTGGCATTCTTGGTTATTCTGTTGTGAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACGGTTACAGAATTCAAGGAATGATTCCACCACATCACAACTGGATTCAAGCAACGACATTCCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGTTAGCAGCCATTCCAGACGACGAGTTAGCAGCAAATGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGATGTGCCGTGTCAGTTGAACGCGATTCAGCTCCCAAATGTCCTGTCTGCCGCCAGCAAATCCGAAGTTCTGTGCGGATTTACTATTCATGA

Coding sequence (CDS)

ATGTCCACATCGGAGCAAGCCTTCGTAACTTTACTCTCCCGCTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCTGCCCTAGCTTATGCTGCCGTTCGTACCGTTCTCAAACTCACTGCTTCCTGTTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTAAGAGTTTCTGATCTTCGTCATATCATTCCCGACGGAGATTCGGGACAATCCGAGTCCGATCGAAAGCTTGTTGTTGTTCGCGGCACCGTGGAGGCCAGGTCTTCTGTGGAAGGTAATTGGAAGAGCTTACAGCCTAATGTATTGATTTCTCAAGAATCCGGAGATAGAGCGGTGATTATTCAGAGGACTCAAGTGGTTCCTTTTGTTCTTGTTGAGGGTCATTCACCATACTCTGATTTTGTTGTCGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCATGAGTACCCTGTTGGGGTGCTGGATGAGGAGAAGATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTGAAAATGGAGTTCCTGTGATAAAGTCGTGCAATGATTTTCCCCATTTCCTATGTGAAATGACTAAGGACCAGATGATTTTAGATCTAGTCTTCAAAACAAGATTTCTATTCTGGAGTAGTATTGTCCTTGGTTCATTGTCTGTTGGCATTCTTGGTTATTCTGTTGTGAGAAATTGGAATAGATGGAAACAATGGAGGCAACATAGACGGTTACAGAATTCAAGGAATGATTCCACCACATCACAACTGGATTCAAGCAACGACATTCCCATTTATGGTGATTTTGGAACTGCATCAGACGACGAGTTAGCAGCCATTCCAGACGACGAGTTAGCAGCAAATGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGATGTGCCGTGTCAGTTGAACGCGATTCAGCTCCCAAATGTCCTGTCTGCCGCCAGCAAATCCGAAGTTCTGTGCGGATTTACTATTCATGA

Protein sequence

MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDLRHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQVVPFVLVEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPVIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWRQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS

Homology

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BLAST of HG10023444 vs. NCBI nr
Match: XP_038897215.1 (E3 ubiquitin-protein ligase SPL2 [Benincasa hispida])

HSP 1 Score: 682.2 bits (1759), Expect = 2.6e-192
Identity = 352/407 (86.49%), Postives = 357/407 (87.71%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MSTSEQA VTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVGVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPN+LISQESGDRAVIIQRTQV
Sbjct: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNILISQESGDRAVIIQRTQV 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLVEGHSPYSDFVVVNMDGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC+DFPHFLCEMTKDQMILDLVFKT+FLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           QHRRLQNS NDSTTSQLDSSNDIPIYGDFGTASDDE+         A VPDGQLCVICL 
Sbjct: 301 QHRRLQNSGNDSTTSQLDSSNDIPIYGDFGTASDDEV---------ATVPDGQLCVICLT 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RRKRSAFIPCGH VCCERCAVS+ERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 RRKRSAFIPCGHFVCCERCAVSIERDSAPKCPVCRQQIRSSVRIYDS 398

[Close]

BLAST of HG10023444 vs. NCBI nr
Match: XP_008462309.1 (PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KAA0059400.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa] >TYK03927.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa])

HSP 1 Score: 681.0 bits (1756), Expect = 5.7e-192
Identity = 348/407 (85.50%), Postives = 360/407 (88.45%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MSTSEQA VTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLV+GHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC+DFPHFLCEMTKDQMILDLVFKT+FLFWSSIVLGSL+VGILGYS VRNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           QHRRLQNSRNDSTTSQLDSSNDIPIYGDF  ASDDE A +PDDEL++NVPDGQLCVICLM
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RRKRSAFIPCGHLVCCERCAVSVERDS+PKCP+CRQQIRSSVRIY S
Sbjct: 361 RRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of HG10023444 vs. NCBI nr
Match: XP_023549225.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.7 bits (1698), Expect = 3.0e-185
Identity = 340/408 (83.33%), Postives = 358/408 (87.75%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+SEQA V+LLS+FAL FDGAVLGAALAYAAVRTVLKLTASCSAL+ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALXFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 ----------------------------------VPFVLV-EGHSPYSDFVVVNMDGSRH 180
                                             VPFVLV EGHSPYSDFVVVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKT+FLFWSS+VLGSLSVG+LGYSVVRNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICL 360
           RQ RRLQ+S NDST SQLD SNDIPIYGDFGTASD+E AA P+DE+++NVPDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNDIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of HG10023444 vs. NCBI nr
Match: KAG7013989.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 658.7 bits (1698), Expect = 3.0e-185
Identity = 340/408 (83.33%), Postives = 359/408 (87.99%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+SEQA V+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCSAL+ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 ----------------------------------VPFVLV-EGHSPYSDFVVVNMDGSRH 180
                                             VPFVLV EGHSPYSDFVVVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKT+FLFWSS+VLGSLSVG+LGYSVVRNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICL 360
           RQ RRLQ+S NDST SQLD SN+IPIYGDFGTASD+E AA P+DE+++NVPDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of HG10023444 vs. NCBI nr
Match: XP_023000260.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 333/407 (81.82%), Postives = 355/407 (87.22%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+SEQA V+L SRFALSFDGA LGAALAYAA RTVLKLTASCSAL+ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RH+  DGDSGQSESD+KLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLVEGHSPYSDFVVVNMDGSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVY QLQPVCATPYTFLQA+FGH+YPVGVLDEEKILPLGKNIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC DFPHFLCEMTKDQM+LDLVFKT+FLFWSSIVLGSLSVG+LG SV+RNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           Q RRLQ+S NDST SQLDSSN+IPIYGDFGTASDDE+A+IPDDEL++NVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RR+RSAFIPCGHLVCCERCA+SVERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of HG10023444 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.3e-93
Identity = 197/413 (47.70%), Postives = 259/413 (62.71%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+ E+A + LL+  ALSFDGA+LG  LA +AV + LK  ++ +AL +++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIIP------DGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++P      + +  +  +D+++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQ---------------------------------VVPFVLV-EGHSPYSDFVVVNM 180
           IQRTQ                                  VPFV+V +     S FV VNM
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGHRFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVNM 180

Query: 181 DGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSF 240
           DGSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI SF
Sbjct: 181 DGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYSF 240

Query: 241 ENGVPVIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWN 300
            NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG +SVGIL Y+ VR WN
Sbjct: 241 NNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTWN 300

Query: 301 RWKQWRQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQL 360
           +WKQW   R L    NDS                     DDE    P+D  A  +PDG+L
Sbjct: 301 KWKQWNHQRELPQRPNDSVV-------------------DDE----PED--ADEIPDGEL 360

Query: 361 CVICLMRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           CVIC+ RR+  AFIPCGH+VCC RCA +VER+  PKCPVC Q IR S+R+YYS
Sbjct: 361 CVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

[Close]

BLAST of HG10023444 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 6.7e-10
Identity = 32/86 (37.21%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 288 DIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAV 347
           DI +  D  +   D++AA+P +E    + + ++C +C+ R     FIPCGHLV C+ CA 
Sbjct: 520 DIFVQQDIRSLPTDDIAALPMEEQLRKLQEERMCKVCMDREVSIVFIPCGHLVVCKDCAP 579

Query: 348 SVERDSAPKCPVCRQQIRSSVRIYYS 374
           S+      KCP+CR  I+ +VR + S
Sbjct: 580 SLR-----KCPICRGTIKGTVRTFLS 600

[Close]

BLAST of HG10023444 vs. ExPASy Swiss-Prot
Match: Q94HV7 (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-08
Identity = 66/259 (25.48%), Postives = 117/259 (45.17%), Query Frame = 0


Query: 118 TQVVPFVLVEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYP- 177
           T+ VP+ L +G         VN+D S+  L L           A P + +Q   G+    
Sbjct: 106 TKEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGF 165

Query: 178 --VGVLDEEKILPLGKNISAVGICSFEN-GVPVIKSCNDFPHFLCEMTKDQMILDLVFKT 237
             +GV   E+++P+G  ++ VG    +  G   I+     P ++  +  DQ+I  L   +
Sbjct: 166 KILGVRHVERVVPIGTPLTVVGEAVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLS 225

Query: 238 RFLFWSSIVLGSLSVGILGYSVVRN-WNRWKQWRQHRRLQNSRNDSTTSQLDSSNDIPIY 297
           R   ++S+ L  L V ++   V+     R +   + RR Q +      +    +   P+ 
Sbjct: 226 RRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDAAARRAK--PVT 285

Query: 298 GDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERD 357
           G  GT+ D             + PD  LCV+CL ++  +AF+ CGH+ CC  C++ +   
Sbjct: 286 GG-GTSRD------------GDTPD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-- 336

Query: 358 SAPKCPVCRQQIRSSVRIY 372
               CP+CR++I+  ++IY
Sbjct: 346 ---TCPLCRERIQQVLKIY 336

[Close]

BLAST of HG10023444 vs. ExPASy Swiss-Prot
Match: P41436 (Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=IAP PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 6.3e-08
Identity = 26/64 (40.62%), Postives = 40/64 (62.50%), Query Frame = 0


Query: 310 ELAANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVR 369
           E    V D +LC IC +      F+PCGH+V C +CA+SV+     KCP+CR+ + S ++
Sbjct: 217 EKEPQVEDSKLCKICYVEECIVCFVPCGHVVACAKCALSVD-----KCPMCRKIVTSVLK 275

Query: 370 IYYS 374
           +Y+S
Sbjct: 277 VYFS 275

[Close]

BLAST of HG10023444 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.1e-07
Identity = 25/62 (40.32%), Postives = 39/62 (62.90%), Query Frame = 0


Query: 312 AANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIY 371
           + ++P+ +LC IC      +AF+PCGH+V C +CA SV      KCP+CR+     +R+Y
Sbjct: 382 STSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVY 438

Query: 372 YS 374
           +S
Sbjct: 442 FS 438

[Close]

BLAST of HG10023444 vs. ExPASy TrEMBL
Match: A0A5D3BWQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001240 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.8e-192
Identity = 348/407 (85.50%), Postives = 360/407 (88.45%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MSTSEQA VTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLV+GHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC+DFPHFLCEMTKDQMILDLVFKT+FLFWSSIVLGSL+VGILGYS VRNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           QHRRLQNSRNDSTTSQLDSSNDIPIYGDF  ASDDE A +PDDEL++NVPDGQLCVICLM
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RRKRSAFIPCGHLVCCERCAVSVERDS+PKCP+CRQQIRSSVRIY S
Sbjct: 361 RRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of HG10023444 vs. ExPASy TrEMBL
Match: A0A1S3CGP0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 2.8e-192
Identity = 348/407 (85.50%), Postives = 360/407 (88.45%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MSTSEQA VTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG+S QSESDRKLVVVRGTVE RSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLV+GHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC+DFPHFLCEMTKDQMILDLVFKT+FLFWSSIVLGSL+VGILGYS VRNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           QHRRLQNSRNDSTTSQLDSSNDIPIYGDF  ASDDE A +PDDEL++NVPDGQLCVICLM
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RRKRSAFIPCGHLVCCERCAVSVERDS+PKCP+CRQQIRSSVRIY S
Sbjct: 361 RRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of HG10023444 vs. ExPASy TrEMBL
Match: A0A0A0K708 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.6e-187
Identity = 342/407 (84.03%), Postives = 356/407 (87.47%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MSTSEQA VTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG S QSESDRKLVVVRGTVE RSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVL+EGHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC+DFPHFLCEMTKDQMILDLVFKT+FLFWSSIVLGSL+VGILGYS  RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDF  ASDDE A +PDDEL+++VPDGQLCVICLM
Sbjct: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RRKRSAFIPCGHLVCCERCAVSVER+S+PKCP+CRQQIRSSVRIY S
Sbjct: 361 RRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of HG10023444 vs. ExPASy TrEMBL
Match: A0A6J1KHU7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.6e-185
Identity = 333/407 (81.82%), Postives = 355/407 (87.22%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+SEQA V+L SRFALSFDGA LGAALAYAA RTVLKLTASCSAL+ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RH+  DGDSGQSESD+KLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 ----------------------------------VPFVLVEGHSPYSDFVVVNMDGSRHP 180
                                             VPFVLVEGHSPYSDFVVVNMDGSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVY QLQPVCATPYTFLQA+FGH+YPVGVLDEEKILPLGKNIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300
           IKSC DFPHFLCEMTKDQM+LDLVFKT+FLFWSSIVLGSLSVG+LG SV+RNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLM 360
           Q RRLQ+S NDST SQLDSSN+IPIYGDFGTASDDE+A+IPDDEL++NVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           RR+RSAFIPCGHLVCCERCA+SVERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of HG10023444 vs. ExPASy TrEMBL
Match: A0A6J1GS48 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.3e-185
Identity = 339/408 (83.09%), Postives = 358/408 (87.75%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+SEQA V+LLS+FALSFDGAVLGAALAYAAVRTVLKLTASCSAL+ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120
           RHII DGDSGQSESD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 ----------------------------------VPFVLV-EGHSPYSDFVVVNMDGSRH 180
                                             VPFVLV EGHSPYSDFVVVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGK ISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFKNGVP 240

Query: 241 VIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKT+FLFWSS+VLGSLSVG+LGYSVVRNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICL 360
           RQ RRLQ+S NDST SQLD SN+IPIYGDFGTASD+E AA P+DE+++NVPDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQIRSSVRIY S
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of HG10023444 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 344.7 bits (883), Expect = 9.0e-95
Identity = 197/413 (47.70%), Postives = 259/413 (62.71%), Query Frame = 0


Query: 1   MSTSEQAFVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVRVSDL 60
           MS+ E+A + LL+  ALSFDGA+LG  LA +AV + LK  ++ +AL +++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIIP------DGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++P      + +  +  +D+++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQ---------------------------------VVPFVLV-EGHSPYSDFVVVNM 180
           IQRTQ                                  VPFV+V +     S FV VNM
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGHRFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVNM 180

Query: 181 DGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSF 240
           DGSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI SF
Sbjct: 181 DGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYSF 240

Query: 241 ENGVPVIKSCNDFPHFLCEMTKDQMILDLVFKTRFLFWSSIVLGSLSVGILGYSVVRNWN 300
            NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG +SVGIL Y+ VR WN
Sbjct: 241 NNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTWN 300

Query: 301 RWKQWRQHRRLQNSRNDSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQL 360
           +WKQW   R L    NDS                     DDE    P+D  A  +PDG+L
Sbjct: 301 KWKQWNHQRELPQRPNDSVV-------------------DDE----PED--ADEIPDGEL 360

Query: 361 CVICLMRRKRSAFIPCGHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRIYYS 374
           CVIC+ RR+  AFIPCGH+VCC RCA +VER+  PKCPVC Q IR S+R+YYS
Sbjct: 361 CVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

[Close]

BLAST of HG10023444 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 9.0e-10
Identity = 66/259 (25.48%), Postives = 117/259 (45.17%), Query Frame = 0


Query: 118 TQVVPFVLVEGHSPYSDFVVVNMDGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYP- 177
           T+ VP+ L +G         VN+D S+  L L           A P + +Q   G+    
Sbjct: 106 TKEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGF 165

Query: 178 --VGVLDEEKILPLGKNISAVGICSFEN-GVPVIKSCNDFPHFLCEMTKDQMILDLVFKT 237
             +GV   E+++P+G  ++ VG    +  G   I+     P ++  +  DQ+I  L   +
Sbjct: 166 KILGVRHVERVVPIGTPLTVVGEAVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLS 225

Query: 238 RFLFWSSIVLGSLSVGILGYSVVRN-WNRWKQWRQHRRLQNSRNDSTTSQLDSSNDIPIY 297
           R   ++S+ L  L V ++   V+     R +   + RR Q +      +    +   P+ 
Sbjct: 226 RRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDAAARRAK--PVT 285

Query: 298 GDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERD 357
           G  GT+ D             + PD  LCV+CL ++  +AF+ CGH+ CC  C++ +   
Sbjct: 286 GG-GTSRD------------GDTPD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-- 336

Query: 358 SAPKCPVCRQQIRSSVRIY 372
               CP+CR++I+  ++IY
Sbjct: 346 ---TCPLCRERIQQVLKIY 336

[Close]

BLAST of HG10023444 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-07
Identity = 31/94 (32.98%), Postives = 45/94 (47.87%), Query Frame = 0


Query: 277 DSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPC 336
           D T   ++ S ++   GD  TAS            + +V    LCVIC   R+   F+PC
Sbjct: 34  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 93

Query: 337 GHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRI 371
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 94  GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 118

[Close]

BLAST of HG10023444 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-07
Identity = 31/94 (32.98%), Postives = 45/94 (47.87%), Query Frame = 0


Query: 277 DSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPC 336
           D T   ++ S ++   GD  TAS            + +V    LCVIC   R+   F+PC
Sbjct: 68  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 127

Query: 337 GHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRI 371
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 128 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 152

[Close]

BLAST of HG10023444 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 2.4e-07
Identity = 31/94 (32.98%), Postives = 45/94 (47.87%), Query Frame = 0


Query: 277 DSTTSQLDSSNDIPIYGDFGTASDDELAAIPDDELAANVPDGQLCVICLMRRKRSAFIPC 336
           D T   ++ S ++   GD  TAS            + +V    LCVIC   R+   F+PC
Sbjct: 89  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 148

Query: 337 GHLVCCERCAVSVERDSAPKCPVCRQQIRSSVRI 371
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 149 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 173

The following BLAST results are available for this feature:

BLAST of HG10023444 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897215.1	2.6e-192	86.49	E3 ubiquitin-protein ligase SPL2 [Benincasa hispida]	[more]
XP_008462309.1	5.7e-192	85.50	PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KA...	[more]
XP_023549225.1	3.0e-185	83.33	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pep...	[more]
KAG7013989.1	3.0e-185	83.33	E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023000260.1	1.2e-184	81.82	E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita maxima]	[more]

BLAST of HG10023444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYH3	1.3e-93	47.70	E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV...	[more]
O08863	6.7e-10	37.21	Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE...	[more]
Q94HV7	1.3e-08	25.48	E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV...	[more]
P41436	6.3e-08	40.63	Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963...	[more]
Q24306	1.1e-07	40.32	Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of HG10023444 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BWQ0	2.8e-192	85.50	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CGP0	2.8e-192	85.50	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 ...	[more]
A0A0A0K708	1.6e-187	84.03	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE...	[more]
A0A6J1KHU7	5.6e-185	81.82	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 P...	[more]
A0A6J1GS48	7.3e-185	83.09	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504...	[more]

BLAST of HG10023444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54150.1	9.0e-95	47.70	E3 Ubiquitin ligase family protein	[more]
AT1G59560.1	9.0e-10	25.48	E3 Ubiquitin ligase family protein	[more]
AT4G24204.1	2.4e-07	32.98	RING/U-box superfamily protein	[more]
AT4G24204.2	2.4e-07	32.98	RING/U-box superfamily protein	[more]
AT4G24204.3	2.4e-07	32.98	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 321..360 e-value: 0.0021 score: 27.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 321..361 score: 10.895954
IPR022170	E3 Ubiquitin ligase MUL1-like	PFAM	PF12483	GIDE	coord: 111..250 e-value: 1.3E-34 score: 118.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 296..373 e-value: 3.8E-17 score: 63.5
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 319..366 e-value: 5.2E-14 score: 51.8
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 320..366
IPR044247	E3 ubiquitin-protein ligase SPL2-like	PANTHER	PTHR47355	E3 UBIQUITIN-PROTEIN LIGASE SPL2	coord: 1..120 coord: 119..373

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10023444.1	HG10023444.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006996	organelle organization
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity