HG10023402 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10023402
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	eIF-2B GDP-GTP exchange factor subunit alpha
Location	Chr05: 33866784 .. 33870289 (+)
RNA-Seq Expression	HG10023402
Synteny	HG10023402

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGTGGAGATCGGCTTCTTTCATCCTCGACAAGCAGCAGGCTGAAGGCGCTTTAAAGCCCCCAGAAACCCTTTCTATCTCCCCACCCGCGCAATCTTCTATGGCGGACGCATTTCAAAACCCTAACCCTAAAATCTCAGCCTATTACCAAAGTAGGGCTGCCCACACCGCCGTCGTCACCAGTGATTGGCTCGCCCAGGCGCAGGCCGCTGTCGGATTCCAAACCGACGATCATATACTGTCGGAAGCTGATGCCAGGGACTCTGAATCCGGTAAGCCGTTCAGTGTGATCGATGAGTTTAATAACTGGAGGAAACAGCCGGATTTGGCCGAGGCTGTTGCAGCTATTCGGGCTTTGGCGGCTGTAATAAGGTCTAGTCAGGCCACGACTATGATGGAGCTCGAAATTGAGCTGAAAAAGGCTTCGGACTCTCTAAAAGTGAGTTATTCTCAGCTTCCATGTTTGTTGTACTGTATTTTTAGGTTTTAAAGGCATAATTGGGAAGTCATTTTTGTTGGAAATGGAGTGGTCTAACAGTATGTGCTGGTCATCCGTTTGTATCATCTTCTATGCTTACTTGGTCTAGAAGTTGTGTTCAAGTGGATGCACGACCATGGGACTCTGCTTTAACTAAGTAGATAGATGCATTTTAGCATAAGCATTATGTTAACGAATGTGTATATTATGTGTATATTATTGTTGTAATCGGTGAAACGGTTGTTACATTTTGATAACTGATGGTACCATGAAAGTCATATTTTTGTTTGCCCAATATATAGAGAAAAGGGGTGCACAAGTTTTGGATAGAGTATAGTTCATACAAAGAATGGTCTTTCCGTGCTTTCTGTTTGATGGGTTAATACAATTTAAACTCTGTCTATCACCTCCAGTCGTGGGATACAACTTCGATATCATTAACAGCTGGCTGTGATTTGTTCTTGCGGTATGTAACTCGAACATCGGCTTTAGAATATGAGGACTTCAAGTCTGCTAAATCTCGTCTGATTGAACGTGCAGAAAAGTTTGGGGAGATATCTTGTAAGGTAGAGCTTATCACGATAATCTTTTGTACTAGTCGAACGCTATATAATTTGACGAGGAATTTTAGTACTAAAACTGGTACTGCGTTGCAGGCACGGAGAATTATTGCAATGCTTAGTCAGGATTTCATTTTTGATGGCTGTACAATTTTGGTCCATGGTTTCTCTAGAGTTGTTATGGAAGTTCTGAGGTTGGCTGCACAGAATAAGAAGCTCTTTAGAGTGTTTTGCACAGGTTCTGCTCTTTTAAGGCTCTAACCACCTTGCAAATTATGTTGTCATTAAATTCTGTATTACTTAGTCAAACTCTAGGAATTTGTGTGTGTGTGTGTGTTTTGAAGGAAGTGGACCTTTCCCTTTGATATACTGGATAAGGGGCCAATTTATTTTGTTTTCTTATGATTTTTTCTTTTGATCGAAAAAGATTCATTTCATTGATAGAATGAAACTACAAAATGATGAAATCCTATCCAGTGGATTGCAAAAAATCTATTCCAATCGGTAGTAAGAGTTGATAAGCTATAATTACAAAAGGAGTTCAATTTACACCAAGTTAGAGATAGATAGTTTATATATTTTTTTCCTTATGAATTTTTCTTTTGATAGGAAAAAAAAATTCATTTCAACAGAGTGAAACGACAAAATGATGAAATCCTATCTAGTGGATTGCAAAAGTCTATTCCAATGTGGTGTAAGGGTTGATAAGCTGTAATTACAAAAGGGGCTCAATTTACACCAAGTCAAGAGATAGATAATTAGTTGATGATGGGAGAGATTGAAAACCTTTTTCTTTTTCGGTGTAATACAAGAGCTTCTTTTCAACCATATAGACCACGAGAAAGCCCCAAAGAGGTTTTAGCCCTAGGATATTCTTTTCTTTCTTGAATGGATGCCCAGTGAACATGAGGGAGAGGACATCTTGAAATTTCGTAAGGTGATTGTGTGCTTCCTCTTCTTTTTTATGTAGAATACAGAAGCTAGGAGAGATACTTATCCATGGAGATCGTCTTTGCAGCTTGTCGTGTGTCAAAGCAATAATGGTTGGGAAAATGGATAAATAATAAATGAGGAGGTTGGCCACTGTTGCTTGTATCAGAGCAATGGCAACCACTAAGGATGTGTGAGTGCCCCCAAGAACTAATTCTTTTTGCCACTTTCTCAACAATAGGAGCCAAAAAGATATAGATTTTGCCTTAACATTGAAGGGAAAACCCGGATATGAGGCTGGCCATTTTTCCTTCTTTGCAACCAAATTTAACCGCCAATGATGAGACTAAGTATGCAGCAAGGTTTATCCCCAAAAATTCAGAACTTTTACCGTTTGTATTAAGCCCAGAAGCTTCCTCAAATGCTATGATGGTGTGAAAGACTCACAGTCAGATCCTCATCCAAGATGTCAAGTACATTGAGTCTGGCAAAGTGCCATTTGGGTACACTAATAGAAGACTGGATAAGGAAAAGGCTAGCAGTTGTGTAAATTATAACTGTAGCTAATGTTACATGTACCAACCTTTCTATCTTTGTTTTTTGAATGTGGGGATAGATGATGGACCAGACATTTAGATGCCCCAGTACAAATCAGCTGCCAGGTTAGTTCAAGAGGGCTTATGTCTCCTCCTACCGCTACCTTTTTCACAGCAAAGATGAATAACAAAACTAACTTACGACGTACTATATAACTTATCTAAAACTTGTTATGTACTATATAAAGTAAGTACTTGGTCTAGCTAACCAGCTATAAAAAGGCACTCTTCCTTTTCTCTTTCTCTAATACACTGAAATAGCAGTATGCTTTACTTCTGGTGAACAGGAAACCAATATTTTTCCTATCATGTCTAGTTCTTCATATTGATGTGGTTTTTATTAGCTTTCATATTTGTTGAAATTCAATGTTAAAATATGCAGAAGGAAGACCAGACAGGACAGGTTTGCGTTTATCCAATGAGCTAGCTAAACTTGATGTCCCTGTGAAGCTCCTAATTGACTCAGCAGTAGCGTATACAATGGATGAGGTTGATATGGTTCTTGTTGGGGCCGATGGAGTAGTTGAGAGTGGAGGTATAATCAACATGATGGGCACGTATCAAATTGCATTAGTGGCACATAGCATGAACAAACCGGTGTATGTGGCCGCTGAGAGTTATAAGGTTTGCCTTCATTAGATGATTTGTAGTATTGGGATTCAGATACCTTCTTACCACTTTATGATTATAGTAACTGGAAAAAAAAAAAACTGACTTGCAAATATTTTTGCAGTTTGCTCGACTGTACCCATTGGATCAAAAAGACATGGCCCCTGCTCTGCGTCCAATAGATTTTGGGGTTCCAATCCCATCCAAGGTTGAAGTTGAAAAATCTGCTCGAGACTATACTCCTCCTCAATACCTTACTCTTCTCTTTACAGATTTGGGCGTTCTCTCTCCATCTGTGGTGAGTGACGAACTCATCCAACTATATTTATAG

mRNA sequence

ATGTGGTGGAGATCGGCTTCTTTCATCCTCGACAAGCAGCAGGCTGAAGGCGCTTTAAAGCCCCCAGAAACCCTTTCTATCTCCCCACCCGCGCAATCTTCTATGGCGGACGCATTTCAAAACCCTAACCCTAAAATCTCAGCCTATTACCAAAGTAGGGCTGCCCACACCGCCGTCGTCACCAGTGATTGGCTCGCCCAGGCGCAGGCCGCTGTCGGATTCCAAACCGACGATCATATACTGTCGGAAGCTGATGCCAGGGACTCTGAATCCGGTAAGCCGTTCAGTGTGATCGATGAGTTTAATAACTGGAGGAAACAGCCGGATTTGGCCGAGGCTGTTGCAGCTATTCGGGCTTTGGCGGCTGTAATAAGGTCTAGTCAGGCCACGACTATGATGGAGCTCGAAATTGAGCTGAAAAAGGCTTCGGACTCTCTAAAATCGTGGGATACAACTTCGATATCATTAACAGCTGGCTGTGATTTGTTCTTGCGGTATGTAACTCGAACATCGGCTTTAGAATATGAGGACTTCAAGTCTGCTAAATCTCGTCTGATTGAACGTGCAGAAAAGTTTGGGGAGATATCTTGTAAGGCACGGAGAATTATTGCAATGCTTAGTCAGGATTTCATTTTTGATGGCTGTACAATTTTGGTCCATGGTTTCTCTAGAGTTGTTATGGAAGTTCTGAGGTTGGCTGCACAGAATAAGAAGCTCTTTAGAGTGTTTTGCACAGAAGGAAGACCAGACAGGACAGGTTTGCGTTTATCCAATGAGCTAGCTAAACTTGATGTCCCTTTTGCTCGACTGTACCCATTGGATCAAAAAGACATGGCCCCTGCTCTGCGTCCAATAGATTTTGGGGTTCCAATCCCATCCAAGGTTGAAGTTGAAAAATCTGCTCGAGACTATACTCCTCCTCAATACCTTACTCTTCTCTTTACAGATTTGGGCGTTCTCTCTCCATCTGTGGTGAGTGACGAACTCATCCAACTATATTTATAG

Coding sequence (CDS)

ATGTGGTGGAGATCGGCTTCTTTCATCCTCGACAAGCAGCAGGCTGAAGGCGCTTTAAAGCCCCCAGAAACCCTTTCTATCTCCCCACCCGCGCAATCTTCTATGGCGGACGCATTTCAAAACCCTAACCCTAAAATCTCAGCCTATTACCAAAGTAGGGCTGCCCACACCGCCGTCGTCACCAGTGATTGGCTCGCCCAGGCGCAGGCCGCTGTCGGATTCCAAACCGACGATCATATACTGTCGGAAGCTGATGCCAGGGACTCTGAATCCGGTAAGCCGTTCAGTGTGATCGATGAGTTTAATAACTGGAGGAAACAGCCGGATTTGGCCGAGGCTGTTGCAGCTATTCGGGCTTTGGCGGCTGTAATAAGGTCTAGTCAGGCCACGACTATGATGGAGCTCGAAATTGAGCTGAAAAAGGCTTCGGACTCTCTAAAATCGTGGGATACAACTTCGATATCATTAACAGCTGGCTGTGATTTGTTCTTGCGGTATGTAACTCGAACATCGGCTTTAGAATATGAGGACTTCAAGTCTGCTAAATCTCGTCTGATTGAACGTGCAGAAAAGTTTGGGGAGATATCTTGTAAGGCACGGAGAATTATTGCAATGCTTAGTCAGGATTTCATTTTTGATGGCTGTACAATTTTGGTCCATGGTTTCTCTAGAGTTGTTATGGAAGTTCTGAGGTTGGCTGCACAGAATAAGAAGCTCTTTAGAGTGTTTTGCACAGAAGGAAGACCAGACAGGACAGGTTTGCGTTTATCCAATGAGCTAGCTAAACTTGATGTCCCTTTTGCTCGACTGTACCCATTGGATCAAAAAGACATGGCCCCTGCTCTGCGTCCAATAGATTTTGGGGTTCCAATCCCATCCAAGGTTGAAGTTGAAAAATCTGCTCGAGACTATACTCCTCCTCAATACCTTACTCTTCTCTTTACAGATTTGGGCGTTCTCTCTCCATCTGTGGTGAGTGACGAACTCATCCAACTATATTTATAG

Protein sequence

MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVVTSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVPFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKSARDYTPPQYLTLLFTDLGVLSPSVVSDELIQLYL

Homology

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BLAST of HG10023402 vs. NCBI nr
Match: XP_038899835.1 (translation initiation factor eIF-2B subunit alpha [Benincasa hispida])

HSP 1 Score: 608.6 bits (1568), Expect = 3.2e-170
Identity = 327/394 (82.99%), Postives = 330/394 (83.76%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEG  KPPETLSISPPAQSSMADAFQ+ NPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGVSKPPETLSISPPAQSSMADAFQHTNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDDHILSE DAR+SESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDHILSETDARNSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
           AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. NCBI nr
Match: XP_008462243.1 (PREDICTED: translation initiation factor eIF-2B subunit alpha [Cucumis melo] >KAA0059358.1 translation initiation factor eIF-2B subunit alpha [Cucumis melo var. makuwa] >TYK03968.1 translation initiation factor eIF-2B subunit alpha [Cucumis melo var. makuwa])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 325/394 (82.49%), Postives = 328/394 (83.25%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEGA KPPETLSISPP QSSMADAFQNPNPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGASKPPETLSISPPTQSSMADAFQNPNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDD +LSE D +DS SGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDQLLSETDTKDSASGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
           AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. NCBI nr
Match: XP_004141730.1 (translation initiation factor eIF-2B subunit alpha [Cucumis sativus] >KGN45441.1 hypothetical protein Csa_015900 [Cucumis sativus])

HSP 1 Score: 603.6 bits (1555), Expect = 1.0e-168
Identity = 325/394 (82.49%), Postives = 326/394 (82.74%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEGA K PETLSISPP QSSMADAFQNPNPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGASKAPETLSISPPTQSSMADAFQNPNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDD I SE D RDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDQIPSETDTRDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
            KSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 GKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYAMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. NCBI nr
Match: XP_023000216.1 (translation initiation factor eIF-2B subunit alpha-like [Cucurbita maxima])

HSP 1 Score: 594.0 bits (1530), Expect = 8.2e-166
Identity = 319/395 (80.76%), Postives = 326/395 (82.53%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPA-QSSMADAFQNPNPKISAYYQSRAAHTAV 60
           MWWRSASFILDKQQ EG  KPPETLSISPP  +SSMAD FQNPNPKISAYYQSRA+HTAV
Sbjct: 1   MWWRSASFILDKQQTEGVSKPPETLSISPPVDESSMADTFQNPNPKISAYYQSRASHTAV 60

Query: 61  VTSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           VTSDWLAQAQAAVGF TD+ +LSEAD +DSESGKPFSVIDEFNNWRKQPDLAEAVAAIR+
Sbjct: 61  VTSDWLAQAQAAVGFPTDEQLLSEADVKDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRS 120

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFK
Sbjct: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFK 180

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL
Sbjct: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRVFCTEGRPDRTGLRLSNELAKLDVP                                 
Sbjct: 241 FRVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGII 300

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK
Sbjct: 301 NMMGTYQIALVAHSMNKPMYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 360

[Close]

BLAST of HG10023402 vs. NCBI nr
Match: XP_022964522.1 (translation initiation factor eIF-2B subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-164
Identity = 318/395 (80.51%), Postives = 324/395 (82.03%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPA-QSSMADAFQNPNPKISAYYQSRAAHTAV 60
           MWWRSASFILDKQQ EG  K PETLSISPP  +SSMAD FQNPNPKISAYYQSRA+HTAV
Sbjct: 1   MWWRSASFILDKQQTEGVSKQPETLSISPPVDESSMADTFQNPNPKISAYYQSRASHTAV 60

Query: 61  VTSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           VTSDWLAQAQAAVGF TD+ +LSEAD RDSESGKPF VIDEFNNWRKQPDLAEAVAAIR+
Sbjct: 61  VTSDWLAQAQAAVGFPTDEQLLSEADVRDSESGKPFCVIDEFNNWRKQPDLAEAVAAIRS 120

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFK
Sbjct: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFK 180

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL
Sbjct: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRVFCTEGRPDRTGLRLSNELAKLDVP                                 
Sbjct: 241 FRVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGII 300

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK
Sbjct: 301 NMMGTYQIALVAHSMNKPMYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 360

[Close]

BLAST of HG10023402 vs. ExPASy Swiss-Prot
Match: Q54I81 (Translation initiation factor eIF-2B subunit alpha OS=Dictyostelium discoideum OX=44689 GN=eif2b1 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 7.0e-35
Identity = 103/301 (34.22%), Postives = 148/301 (49.17%), Query Frame = 0


Query: 105 RKQPDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWD-TTSISLTAGCDLF 164
           +++ +++ A+  IR L  VIR S +TT++ L+ EL+ A   LKS     SISL++ CD F
Sbjct: 21  KRETEVSIAIPTIRILIDVIRKSNSTTVVGLQKELEDAVKQLKSCPYNQSISLSSVCDSF 80

Query: 165 LRYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFS 224
           +R+VT+ + L++ +F + KS L+ER E+    S  +R  I+ L+  FI DG TILVHGFS
Sbjct: 81  IRFVTKRTELDFPNFDTCKSNLVERGEQLSNKSSMSRTKISQLADKFIRDGVTILVHGFS 140

Query: 225 RVVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVP----------------- 284
           RVV+ +L  AA   K F V  TE RPD +G + +  L   ++P                 
Sbjct: 141 RVVLGLLLHAAFQGKRFSVIVTESRPDSSGYKTAARLQAANIPVKLIMDGGVSRIIDKVD 200

Query: 285 -------------------------------------------FARLYPLDQKDMAPALR 334
                                                      F R YPL+Q D+   L+
Sbjct: 201 YVLVGAEAIVENGGIVNKIGTYQISIVAKAFKKPFYVAAESFKFTRSYPLNQSDI-ENLK 260

[Close]

BLAST of HG10023402 vs. ExPASy Swiss-Prot
Match: Q4R4V8 (Translation initiation factor eIF-2B subunit alpha OS=Macaca fascicularis OX=9541 GN=EIF2B1 PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-32
Identity = 105/306 (34.31%), Postives = 148/306 (48.37%), Query Frame = 0


Query: 97  VIDEFNNWRKQ-PDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSIS 156
           +I+ F +  K+ PD+A AVAAIR L   ++  +  T+  L   L  A ++L   D +S++
Sbjct: 6   LIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVD-SSVA 65

Query: 157 LTAGCDLFLRYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGC 216
           +++G +LFLR+++ TS LEY D+   K  +IER E F      +R  IA L   FI DG 
Sbjct: 66  VSSGGELFLRFISLTS-LEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGA 125

Query: 217 TILVHGFSRVVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVP--------- 276
           TIL H +SRVV+ VL  A   KK F V+ TE +PD +G +++  L  L+VP         
Sbjct: 126 TILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAV 185

Query: 277 ---------------------------------------------------FARLYPLDQ 335
                                                              F RL+PL+Q
Sbjct: 186 GYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYAVAESFKFVRLFPLNQ 245

[Close]

BLAST of HG10023402 vs. ExPASy Swiss-Prot
Match: Q0IIF2 (Translation initiation factor eIF-2B subunit alpha OS=Bos taurus OX=9913 GN=EIF2B1 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 7.3e-32
Identity = 102/297 (34.34%), Postives = 141/297 (47.47%), Query Frame = 0


Query: 105 RKQPDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFL 164
           ++ PD+A AVAAIR L   +R     T+  L   L  A ++L   D +S+++++G +LFL
Sbjct: 15  KEDPDMASAVAAIRTLLEYLRRDTGETIQGLRANLTSAIETLCGVD-SSVAVSSGGELFL 74

Query: 165 RYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSR 224
           R+++ TS LEY D+   K  +IER E F      +R  IA L   FI DG  IL H +SR
Sbjct: 75  RFISLTS-LEYSDYSKCKKIMIERGEIFLRRISLSRNKIADLCHTFIKDGARILTHAYSR 134

Query: 225 VVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVP------------------ 284
           VV+ VL  A   KK F V+ TE +PD +G +++  L  L+VP                  
Sbjct: 135 VVLRVLEAAVAAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKVDL 194

Query: 285 ------------------------------------------FARLYPLDQKDM------ 335
                                                     F RL+PL+Q+D+      
Sbjct: 195 VIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKY 254

[Close]

BLAST of HG10023402 vs. ExPASy Swiss-Prot
Match: Q14232 (Translation initiation factor eIF-2B subunit alpha OS=Homo sapiens OX=9606 GN=EIF2B1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.5e-32
Identity = 104/306 (33.99%), Postives = 147/306 (48.04%), Query Frame = 0


Query: 97  VIDEFNNWRKQ-PDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSIS 156
           +I+ F +  K+ PD+A AVAAIR L   ++  +  T+  L   L  A ++L   D +S++
Sbjct: 6   LIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVD-SSVA 65

Query: 157 LTAGCDLFLRYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGC 216
           +++G +LFLR+++  S LEY D+   K  +IER E F      +R  IA L   FI DG 
Sbjct: 66  VSSGGELFLRFISLAS-LEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGA 125

Query: 217 TILVHGFSRVVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVP--------- 276
           TIL H +SRVV+ VL  A   KK F V+ TE +PD +G +++  L  L+VP         
Sbjct: 126 TILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAV 185

Query: 277 ---------------------------------------------------FARLYPLDQ 335
                                                              F RL+PL+Q
Sbjct: 186 GYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQ 245

[Close]

BLAST of HG10023402 vs. ExPASy Swiss-Prot
Match: Q5RAR0 (Translation initiation factor eIF-2B subunit alpha OS=Pongo abelii OX=9601 GN=EIF2B1 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 9.5e-32
Identity = 104/306 (33.99%), Postives = 147/306 (48.04%), Query Frame = 0


Query: 97  VIDEFNNWRKQ-PDLAEAVAAIRALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSIS 156
           +I+ F +  K+ PD+A AVAAIR L   ++  +  T+  L   L  A ++L   D +S++
Sbjct: 6   LIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVD-SSVA 65

Query: 157 LTAGCDLFLRYVTRTSALEYEDFKSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGC 216
           +++G +LFLR+++  S LEY D+   K  +IER E F      +R  IA L   FI DG 
Sbjct: 66  VSSGGELFLRFISLAS-LEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGA 125

Query: 217 TILVHGFSRVVMEVLRLAAQNKKLFRVFCTEGRPDRTGLRLSNELAKLDVP--------- 276
           TIL H +SRVV+ VL  A   KK F V+ TE +PD +G +++  L  L+VP         
Sbjct: 126 TILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAV 185

Query: 277 ---------------------------------------------------FARLYPLDQ 335
                                                              F RL+PL+Q
Sbjct: 186 GYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQ 245

[Close]

BLAST of HG10023402 vs. ExPASy TrEMBL
Match: A0A5A7UW24 (eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001650 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.9e-170
Identity = 325/394 (82.49%), Postives = 328/394 (83.25%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEGA KPPETLSISPP QSSMADAFQNPNPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGASKPPETLSISPPTQSSMADAFQNPNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDD +LSE D +DS SGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDQLLSETDTKDSASGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
           AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. ExPASy TrEMBL
Match: A0A1S3CGI1 (eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis melo OX=3656 GN=LOC103500646 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.9e-170
Identity = 325/394 (82.49%), Postives = 328/394 (83.25%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEGA KPPETLSISPP QSSMADAFQNPNPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGASKPPETLSISPPTQSSMADAFQNPNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDD +LSE D +DS SGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDQLLSETDTKDSASGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
           AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. ExPASy TrEMBL
Match: A0A0A0KCC1 (eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_7G448030 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.0e-169
Identity = 325/394 (82.49%), Postives = 326/394 (82.74%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MWWRSASFILDKQQAEGA K PETLSISPP QSSMADAFQNPNPKISAYYQSRAAHTAVV
Sbjct: 1   MWWRSASFILDKQQAEGASKAPETLSISPPTQSSMADAFQNPNPKISAYYQSRAAHTAVV 60

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120
           TSDWLAQAQAAVGFQTDD I SE D RDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL
Sbjct: 61  TSDWLAQAQAAVGFQTDDQIPSETDTRDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRAL 120

Query: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFKS 180
           AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFKS
Sbjct: 121 AAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFKS 180

Query: 181 AKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240
            KSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF
Sbjct: 181 GKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKLF 240

Query: 241 RVFCTEGRPDRTGLRLSNELAKLDVP---------------------------------- 300
           RVFCTEGRPDRTGLRLSNELAKLDVP                                  
Sbjct: 241 RVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYAMDEVDMVLVGADGVVESGGIIN 300

Query: 301 --------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 335
                                     FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS
Sbjct: 301 MMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEKS 360

[Close]

BLAST of HG10023402 vs. ExPASy TrEMBL
Match: A0A6J1KF96 (eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111494505 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.0e-166
Identity = 319/395 (80.76%), Postives = 326/395 (82.53%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPA-QSSMADAFQNPNPKISAYYQSRAAHTAV 60
           MWWRSASFILDKQQ EG  KPPETLSISPP  +SSMAD FQNPNPKISAYYQSRA+HTAV
Sbjct: 1   MWWRSASFILDKQQTEGVSKPPETLSISPPVDESSMADTFQNPNPKISAYYQSRASHTAV 60

Query: 61  VTSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           VTSDWLAQAQAAVGF TD+ +LSEAD +DSESGKPFSVIDEFNNWRKQPDLAEAVAAIR+
Sbjct: 61  VTSDWLAQAQAAVGFPTDEQLLSEADVKDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRS 120

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFK
Sbjct: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFK 180

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL
Sbjct: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRVFCTEGRPDRTGLRLSNELAKLDVP                                 
Sbjct: 241 FRVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGII 300

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK
Sbjct: 301 NMMGTYQIALVAHSMNKPMYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 360

[Close]

BLAST of HG10023402 vs. ExPASy TrEMBL
Match: A0A6J1HI10 (eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111464524 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.7e-165
Identity = 318/395 (80.51%), Postives = 324/395 (82.03%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPA-QSSMADAFQNPNPKISAYYQSRAAHTAV 60
           MWWRSASFILDKQQ EG  K PETLSISPP  +SSMAD FQNPNPKISAYYQSRA+HTAV
Sbjct: 1   MWWRSASFILDKQQTEGVSKQPETLSISPPVDESSMADTFQNPNPKISAYYQSRASHTAV 60

Query: 61  VTSDWLAQAQAAVGFQTDDHILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           VTSDWLAQAQAAVGF TD+ +LSEAD RDSESGKPF VIDEFNNWRKQPDLAEAVAAIR+
Sbjct: 61  VTSDWLAQAQAAVGFPTDEQLLSEADVRDSESGKPFCVIDEFNNWRKQPDLAEAVAAIRS 120

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLF+RYVTRTSALEYEDFK
Sbjct: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFMRYVTRTSALEYEDFK 180

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL
Sbjct: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRVFCTEGRPDRTGLRLSNELAKLDVP                                 
Sbjct: 241 FRVFCTEGRPDRTGLRLSNELAKLDVPVKLLIDSAVAYTMDEVDMVLVGADGVVESGGII 300

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK
Sbjct: 301 NMMGTYQIALVAHSMNKPMYVAAESYKFARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 360

[Close]

BLAST of HG10023402 vs. TAIR 10
Match: AT1G53880.1 (Eukaryotic translation initiation factor 2B (eIF-2B) family protein )

HSP 1 Score: 466.8 bits (1200), Expect = 1.4e-131
Identity = 258/395 (65.32%), Postives = 288/395 (72.91%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MW  SASFILDK Q    +    ++   PP  +SMAD   NPNP ISAYYQ+RAAH  +V
Sbjct: 196 MWRESASFILDKHQNNKPISLTHSIDSPPPPSASMADENPNPNP-ISAYYQTRAAHHGIV 255

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDS-ESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           TS+WL QAQAAV    D         RDS  SG+PFSVI++FN+WR+QPDLAEAVAAIRA
Sbjct: 256 TSEWLEQAQAAVRRYPD---------RDSLVSGRPFSVIEDFNSWRQQPDLAEAVAAIRA 315

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIR+S+ATTMMELEIELKKASD+LKSWDTTSISLTAGCDLF+RYVTRTSALE+EDF 
Sbjct: 316 LAAVIRASEATTMMELEIELKKASDTLKSWDTTSISLTAGCDLFMRYVTRTSALEFEDFN 375

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSR++ERAEKFGEISCKAR IIAMLSQDFIFDGCTILVHGFSRVV E+L+ +AQNKKL
Sbjct: 376 SAKSRVLERAEKFGEISCKARTIIAMLSQDFIFDGCTILVHGFSRVVFEILKTSAQNKKL 435

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRV CTEGRPD+TG+ L+NELAKLD+P                                 
Sbjct: 436 FRVLCTEGRPDKTGVLLANELAKLDIPVKLLIDSAVAYSMDEVDMVFVGADGVVESGGII 495

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKD+ PALRPIDF VP+P KVEVE+
Sbjct: 496 NMMGTYQIALVAQSMNKPVYVAAESYKFARLYPLDQKDLEPALRPIDFSVPVPPKVEVER 555

[Close]

BLAST of HG10023402 vs. TAIR 10
Match: AT1G53900.1 (Eukaryotic translation initiation factor 2B (eIF-2B) family protein )

HSP 1 Score: 466.8 bits (1200), Expect = 1.4e-131
Identity = 258/395 (65.32%), Postives = 288/395 (72.91%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MW  SASFILDK Q    +    ++   PP  +SMAD   NPNP ISAYYQ+RAAH  +V
Sbjct: 196 MWRESASFILDKHQNNKPISLTHSIDSPPPPSASMADENPNPNP-ISAYYQTRAAHHGIV 255

Query: 61  TSDWLAQAQAAVGFQTDDHILSEADARDS-ESGKPFSVIDEFNNWRKQPDLAEAVAAIRA 120
           TS+WL QAQAAV    D         RDS  SG+PFSVI++FN+WR+QPDLAEAVAAIRA
Sbjct: 256 TSEWLEQAQAAVRRYPD---------RDSLVSGRPFSVIEDFNSWRQQPDLAEAVAAIRA 315

Query: 121 LAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDFK 180
           LAAVIR+S+ATTMMELEIELKKASD+LKSWDTTSISLTAGCDLF+RYVTRTSALE+EDF 
Sbjct: 316 LAAVIRASEATTMMELEIELKKASDTLKSWDTTSISLTAGCDLFMRYVTRTSALEFEDFN 375

Query: 181 SAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKKL 240
           SAKSR++ERAEKFGEISCKAR IIAMLSQDFIFDGCTILVHGFSRVV E+L+ +AQNKKL
Sbjct: 376 SAKSRVLERAEKFGEISCKARTIIAMLSQDFIFDGCTILVHGFSRVVFEILKTSAQNKKL 435

Query: 241 FRVFCTEGRPDRTGLRLSNELAKLDVP--------------------------------- 300
           FRV CTEGRPD+TG+ L+NELAKLD+P                                 
Sbjct: 436 FRVLCTEGRPDKTGVLLANELAKLDIPVKLLIDSAVAYSMDEVDMVFVGADGVVESGGII 495

Query: 301 ---------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVEK 335
                                      FARLYPLDQKD+ PALRPIDF VP+P KVEVE+
Sbjct: 496 NMMGTYQIALVAQSMNKPVYVAAESYKFARLYPLDQKDLEPALRPIDFSVPVPPKVEVER 555

[Close]

BLAST of HG10023402 vs. TAIR 10
Match: AT1G72340.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 459.1 bits (1180), Expect = 2.9e-129
Identity = 260/396 (65.66%), Postives = 289/396 (72.98%), Query Frame = 0


Query: 1   MWWRSASFILDKQQAEGALKPPETLSISPPAQSSMADAFQNPNPKISAYYQSRAAHTAVV 60
           MW RS SFILD++++          S SPP   +    FQNP+  ISAYYQ+RAAH  V+
Sbjct: 1   MWRRSPSFILDERRS----------SNSPPMADTTRGPFQNPD-SISAYYQTRAAHHGVI 60

Query: 61  TSDWLAQAQAAVGFQTDD--HILSEADARDSESGKPFSVIDEFNNWRKQPDLAEAVAAIR 120
           TSDWLAQAQAAVG  + D  H LS  D  + +S   F+VI+EFNNWRKQPDLAEAVAAIR
Sbjct: 61  TSDWLAQAQAAVGGVSGDAQHDLSVTDLGNEKS---FNVIEEFNNWRKQPDLAEAVAAIR 120

Query: 121 ALAAVIRSSQATTMMELEIELKKASDSLKSWDTTSISLTAGCDLFLRYVTRTSALEYEDF 180
           ALAAVIR+S+A+TMMELEIELKKASD+LKSWD TSISLTAGCDLF+RYVTRTSALEYEDF
Sbjct: 121 ALAAVIRASEASTMMELEIELKKASDTLKSWDKTSISLTAGCDLFIRYVTRTSALEYEDF 180

Query: 181 KSAKSRLIERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGFSRVVMEVLRLAAQNKK 240
            SAKSRL+ERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHG SRVV+E+L+ AAQN K
Sbjct: 181 NSAKSRLLERAEKFGEISCKARRIIAMLSQDFIFDGCTILVHGLSRVVLEILKTAAQNNK 240

Query: 241 LFRVFCTEGRPDRTGLRLSNELAKLDVP-------------------------------- 300
           LFRV CTEGRPD TG+ LS+EL+KLD+P                                
Sbjct: 241 LFRVLCTEGRPDGTGVLLSSELSKLDIPVKLLLDSAVAYSMDEVDMVFVGADGVVESGGI 300

Query: 301 ----------------------------FARLYPLDQKDMAPALRPIDFGVPIPSKVEVE 335
                                       FARLYPLDQKDMAPALRPI+FGV IP+KVEVE
Sbjct: 301 INMMGTYQIALVAHSMNKPVYVAAESYKFARLYPLDQKDMAPALRPIEFGVKIPTKVEVE 360

The following BLAST results are available for this feature:

BLAST of HG10023402 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899835.1	3.2e-170	82.99	translation initiation factor eIF-2B subunit alpha [Benincasa hispida]	[more]
XP_008462243.1	1.2e-169	82.49	PREDICTED: translation initiation factor eIF-2B subunit alpha [Cucumis melo] >KA...	[more]
XP_004141730.1	1.0e-168	82.49	translation initiation factor eIF-2B subunit alpha [Cucumis sativus] >KGN45441.1...	[more]
XP_023000216.1	8.2e-166	80.76	translation initiation factor eIF-2B subunit alpha-like [Cucurbita maxima]	[more]
XP_022964522.1	1.2e-164	80.51	translation initiation factor eIF-2B subunit alpha-like [Cucurbita moschata]	[more]

BLAST of HG10023402 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54I81	7.0e-35	34.22	Translation initiation factor eIF-2B subunit alpha OS=Dictyostelium discoideum O...	[more]
Q4R4V8	2.5e-32	34.31	Translation initiation factor eIF-2B subunit alpha OS=Macaca fascicularis OX=954...	[more]
Q0IIF2	7.3e-32	34.34	Translation initiation factor eIF-2B subunit alpha OS=Bos taurus OX=9913 GN=EIF2...	[more]
Q14232	9.5e-32	33.99	Translation initiation factor eIF-2B subunit alpha OS=Homo sapiens OX=9606 GN=EI...	[more]
Q5RAR0	9.5e-32	33.99	Translation initiation factor eIF-2B subunit alpha OS=Pongo abelii OX=9601 GN=EI...	[more]

BLAST of HG10023402 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UW24	5.9e-170	82.49	eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3CGI1	5.9e-170	82.49	eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A0A0KCC1	5.0e-169	82.49	eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_7...	[more]
A0A6J1KF96	4.0e-166	80.76	eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1HI10	5.7e-165	80.51	eIF-2B GDP-GTP exchange factor subunit alpha OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of HG10023402 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G53880.1	1.4e-131	65.32	Eukaryotic translation initiation factor 2B (eIF-2B) family protein	[more]
AT1G53900.1	1.4e-131	65.32	Eukaryotic translation initiation factor 2B (eIF-2B) family protein	[more]
AT1G72340.1	2.9e-129	65.66	NagB/RpiA/CoA transferase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 132..152
None	No IPR available	PANTHER	PTHR45860:SF3	NAGB/RPIA/COA TRANSFERASE-LIKE SUPERFAMILY PROTEIN	coord: 267..334
None	No IPR available	PANTHER	PTHR45860	TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA	coord: 267..334
None	No IPR available	PANTHER	PTHR45860	TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA	coord: 38..267
None	No IPR available	PANTHER	PTHR45860:SF3	NAGB/RPIA/COA TRANSFERASE-LIKE SUPERFAMILY PROTEIN	coord: 38..267
IPR000649	Initiation factor 2B-related	PFAM	PF01008	IF-2B	coord: 108..267 e-value: 5.0E-26 score: 91.6
IPR042528	Translation initiation factor eIF-2B subunit alpha, N-terminal	GENE3D	1.20.120.1070		coord: 94..195 e-value: 2.0E-33 score: 116.4
IPR042529	Initiation factor 2B-like, C-terminal	GENE3D	3.40.50.10470		coord: 197..267 e-value: 4.2E-15 score: 57.4 coord: 268..334 e-value: 4.1E-9 score: 37.9
IPR037171	NagB/RpiA transferase-like	SUPERFAMILY	100950	NagB/RpiA/CoA transferase-like	coord: 96..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10023402.1	HG10023402.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0006413	translational initiation
biological_process	GO:0044237	cellular metabolic process
cellular_component	GO:0005851	eukaryotic translation initiation factor 2B complex
molecular_function	GO:0005085	guanyl-nucleotide exchange factor activity
molecular_function	GO:0003743	translation initiation factor activity