HG10023368 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023368 vs. NCBI nr
Match: XP_038899989.1 (tRNase Z TRZ3, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 1710.3 bits (4428), Expect = 0.0e+00
Identity = 879/984 (89.33%), Postives = 905/984 (91.97%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLSSLRFLFLSS PALSPSKFPFFPS+YSPKSP LFTV+ASS PKRRRSTAAP SL
Sbjct: 1   MPLPHLSSLRFLFLSSSPALSPSKFPFFPSLYSPKSPFLFTVLASSSPKRRRSTAAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           +FKR+NSSSLRET+GKEKSV ME+TE TSFGFNKRRAEG+DK+D PKKNLQLKVRKLNPA
Sbjct: 61  DFKRRNSSSLRETKGKEKSVPMEETEATSFGFNKRRAEGRDKSDLPKKNLQLKVRKLNPA 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL
Sbjct: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
           SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPR NRGFQ FDEDDMG TQEERRNHDS
Sbjct: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRSNRGFQLFDEDDMGWTQEERRNHDS 300

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360
           EARSST KTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS
Sbjct: 301 EARSSTTKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360

Query: 361 DHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVV 420
           D Q IMVHPSDVLGPSVPGPVVLLVDCPTE+HL ELM+LQSL+PYYEDF S++TETGKVV
Sbjct: 361 DRQNIMVHPSDVLGPSVPGPVVLLVDCPTESHLSELMALQSLKPYYEDFLSDETETGKVV 420

Query: 421 TCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480
           TCVIHLSPASIL SPNYQKW KR G AQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ
Sbjct: 421 TCVIHLSPASILSSPNYQKWVKRLGSAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480

Query: 481 LFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 540
           LFPAPGF SHQQLT PGSDSCASTEIEVSNH+KSTLAENLLKFTLRPYAQLGLDRSSIPS
Sbjct: 481 LFPAPGFLSHQQLTIPGSDSCASTEIEVSNHYKSTLAENLLKFTLRPYAQLGLDRSSIPS 540

Query: 541 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 600
           QE++ E I+ALHS+IPEI+DAV HVSQLW+GSAETDEKTP EDNNAMVEEPWLDENKVPS
Sbjct: 541 QETMSEIINALHSEIPEIVDAVDHVSQLWQGSAETDEKTPVEDNNAMVEEPWLDENKVPS 600

Query: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 660
           CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRYGVE
Sbjct: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRYGVE 660

Query: 661 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 720
           GADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVLVIGPRQLKRYLDAYQ
Sbjct: 661 GADAAVRSLRCIWISHIHADHHTGLARILALRRDLLQEVPHEPVLVIGPRQLKRYLDAYQ 720

Query: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 780
           RLEDLDMQFLDCKHTTQASLEAFQNVASEI+K PSES  SST GSST I  A  QKT SS
Sbjct: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIEKLPSESTTSSTYGSSTLIGGAIEQKTASS 780

Query: 781 LFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840
           LFVKGSRMQSYWKGPSSPVDIS AMPLLKCLN+VLNEAGLEAL+SFPVVHCPQAYGVVLK
Sbjct: 781 LFVKGSRMQSYWKGPSSPVDISVAMPLLKCLNKVLNEAGLEALVSFPVVHCPQAYGVVLK 840

Query: 841 AAGR--------------------------------------ATFEDSLVDEAMAKNHST 900
           AA R                                      ATFEDSLVDEAMAKNHST
Sbjct: 841 AAERVNLDGKVIPGWKIIYSGDTRPCPELMEASRGATLLIHEATFEDSLVDEAMAKNHST 900

Query: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVL 947
           TSEAIDIGNSAGAYRIILTHFSQRYPKIPVV ERHMHKTCIAFDLMSVNVADLLVLPKVL
Sbjct: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVDERHMHKTCIAFDLMSVNVADLLVLPKVL 960

BLAST of HG10023368 vs. NCBI nr
Match: XP_011659574.1 (tRNase Z TRZ3, mitochondrial [Cucumis sativus] >KGN45397.1 hypothetical protein Csa_016722 [Cucumis sativus])

HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 851/984 (86.48%), Postives = 890/984 (90.45%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLF       SPSK PF PS+YSPKS SLFTV+ASSPPKRRRS  AP SL
Sbjct: 1   MPLPHLSTLRFLF------FSPSKLPFSPSLYSPKSHSLFTVLASSPPKRRRSATAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR+NSSSLRET+GKE SV ME+TE TSFGFNKRRAEG+DKTD PKKNLQLKVRKLNPA
Sbjct: 61  NFKRRNSSSLRETKGKENSVPMEETEATSFGFNKRRAEGRDKTDLPKKNLQLKVRKLNPA 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGP +
Sbjct: 181 VCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPTV 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
           SSD DAVHELSK  +PIVLVDDEVVKISAILVHPRPNR    FDEDDMG +QE+RRN +S
Sbjct: 241 SSDADAVHELSKCREPIVLVDDEVVKISAILVHPRPNRRLGLFDEDDMGHSQEQRRNDNS 300

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360
           EARSST KTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAA GLKPGPKYRELQLGNSVMS
Sbjct: 301 EARSSTTKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAALGLKPGPKYRELQLGNSVMS 360

Query: 361 DHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVV 420
           DHQKIMVHPSDVLGPSVPGPVVLL+DCPTE+HL ELMSL+SLRPYYED SS+QTETGKVV
Sbjct: 361 DHQKIMVHPSDVLGPSVPGPVVLLIDCPTESHLSELMSLESLRPYYEDLSSDQTETGKVV 420

Query: 421 TCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480
           TCVIHLSPASILG+PNYQKWA+RF  AQHIMAGH RKNVAIPILR+SAKIAARLNHLCPQ
Sbjct: 421 TCVIHLSPASILGNPNYQKWARRFESAQHIMAGHHRKNVAIPILRASAKIAARLNHLCPQ 480

Query: 481 LFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 540
           LFPAPGFWSHQQLT PGSDSCASTEIEVSNH+KSTLAENLLKFTLRPYAQLG DRS+IPS
Sbjct: 481 LFPAPGFWSHQQLTMPGSDSCASTEIEVSNHYKSTLAENLLKFTLRPYAQLGFDRSNIPS 540

Query: 541 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 600
           QESLPE I+ALHS+IPEI+DAV HVSQLWRGSAETDE+TP E+NNAMVEEPWLDENKVPS
Sbjct: 541 QESLPEIINALHSEIPEIVDAVEHVSQLWRGSAETDERTPVEENNAMVEEPWLDENKVPS 600

Query: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 660
           CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRYGVE
Sbjct: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRYGVE 660

Query: 661 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 720
           GADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVLVIGPRQL+RYL+AYQ
Sbjct: 661 GADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVLVIGPRQLRRYLNAYQ 720

Query: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 780
           RLEDLDMQFLDCK TT+ASLEAFQ +AS+ID SPSESPISSTN +ST ID   G+KT SS
Sbjct: 721 RLEDLDMQFLDCKDTTEASLEAFQKLASDIDNSPSESPISSTNENSTLIDGTIGRKTESS 780

Query: 781 LFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840
           LFVKGSRMQSYWKGPSSPVDI+AA+PLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK
Sbjct: 781 LFVKGSRMQSYWKGPSSPVDINAAVPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840

Query: 841 AAGR--------------------------------------ATFEDSLVDEAMAKNHST 900
           AA R                                      ATFEDSLVDEAMAKNHST
Sbjct: 841 AAERVNLDGKVIPGWKIVYSGDTRPCPKLMEASRGATLLIHEATFEDSLVDEAMAKNHST 900

Query: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVL 947
           TSEAIDIGNSAGAYRIILTHFSQRYPKIPVV E+HMHKTCIAFDLMSVNVADL VLPKVL
Sbjct: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVDEKHMHKTCIAFDLMSVNVADLSVLPKVL 960

BLAST of HG10023368 vs. NCBI nr
Match: XP_008462180.1 (PREDICTED: zinc phosphodiesterase ELAC protein 2-like [Cucumis melo])

HSP 1 Score: 1652.9 bits (4279), Expect = 0.0e+00
Identity = 850/984 (86.38%), Postives = 885/984 (89.94%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPH S+LRFLF       SPSK PFFPS+YSPKS SLFTV+ASSPPKRRRST AP SL
Sbjct: 1   MPLPHPSTLRFLF------FSPSKLPFFPSLYSPKSHSLFTVLASSPPKRRRSTTAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR NSSS RET+GKE SV ME+TEGTSFGFNK+RAEG+DKTD PKKNLQLKVRKLNP 
Sbjct: 61  NFKRTNSSSFRETKGKENSVPMEETEGTSFGFNKKRAEGRDKTDLPKKNLQLKVRKLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGP +
Sbjct: 181 VCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPTV 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
            SD DAV ELSK  DPIVLVDDEVVKISAILVHPRPN G   FDEDDMG +QE+RRNH+S
Sbjct: 241 HSDADAVDELSKCRDPIVLVDDEVVKISAILVHPRPNGGLGLFDEDDMGHSQEQRRNHNS 300

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360
           EARS T  TSST+KPGDMSVVYVCELPEIKGKFDPSKAAA GLKPGPKYRELQLGNSVMS
Sbjct: 301 EARSLTTNTSSTIKPGDMSVVYVCELPEIKGKFDPSKAAALGLKPGPKYRELQLGNSVMS 360

Query: 361 DHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVV 420
           D QKIMVHPSDVLGPSVPGPVVLLVDCPTE+HL ELMSL+SLRPYYEDFSS+QTE GKVV
Sbjct: 361 DRQKIMVHPSDVLGPSVPGPVVLLVDCPTESHLSELMSLESLRPYYEDFSSDQTEIGKVV 420

Query: 421 TCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480
           TCVIHLSPASILG+PNYQKWAKRFG AQHIMAGHDRKNVAIPILR+SAKIAARLNHLCPQ
Sbjct: 421 TCVIHLSPASILGNPNYQKWAKRFGSAQHIMAGHDRKNVAIPILRASAKIAARLNHLCPQ 480

Query: 481 LFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 540
           LFPAPGFWSHQQLT PGSDSCA TEIEVSNH+KSTLAENLLKFTLRPYAQLGLDRS+IPS
Sbjct: 481 LFPAPGFWSHQQLTMPGSDSCALTEIEVSNHYKSTLAENLLKFTLRPYAQLGLDRSNIPS 540

Query: 541 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 600
           QESLPE ISALHS+IPEI+DAV HV QLWRGSAETDE+TP E+NNAMVEEPWL+ENKVPS
Sbjct: 541 QESLPEIISALHSEIPEIVDAVEHVGQLWRGSAETDERTPVEENNAMVEEPWLNENKVPS 600

Query: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 660
           CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRYGVE
Sbjct: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRYGVE 660

Query: 661 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 720
           GADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVLVIGPRQLKRYL+AYQ
Sbjct: 661 GADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVLVIGPRQLKRYLNAYQ 720

Query: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 780
           RLEDLDMQFLDCK TT+ASLEAFQNVASEID SPSESPISSTN +ST ID A  +KT +S
Sbjct: 721 RLEDLDMQFLDCKDTTEASLEAFQNVASEIDYSPSESPISSTNENSTLIDGAIRRKTEAS 780

Query: 781 LFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840
           LFVKGSRMQSYWKGPSSPVDIS A+PLLK LNEVLNEAGLEALISFPVVHCPQAYGVVLK
Sbjct: 781 LFVKGSRMQSYWKGPSSPVDISTAVPLLKRLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840

Query: 841 AAGR--------------------------------------ATFEDSLVDEAMAKNHST 900
           AA R                                      ATFEDSLVDEAMAKNHST
Sbjct: 841 AAERVNLDGKGIPGWKIVYSGDTRPCPKLMEASRGATLLIHEATFEDSLVDEAMAKNHST 900

Query: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVL 947
           TSEAIDIGNS GAYRIILTHFSQRYPKIPV+ E+HMHKTCIAFDLMSVNVADL VLPKVL
Sbjct: 901 TSEAIDIGNSVGAYRIILTHFSQRYPKIPVLDEKHMHKTCIAFDLMSVNVADLSVLPKVL 960

BLAST of HG10023368 vs. NCBI nr
Match: TYK04006.1 (zinc phosphodiesterase ELAC protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1636.3 bits (4236), Expect = 0.0e+00
Identity = 850/1001 (84.92%), Postives = 886/1001 (88.51%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLF       SPSK PFFPS+YSPKS SLFTV+ASSPPKRRRST AP SL
Sbjct: 1   MPLPHLSTLRFLF------FSPSKLPFFPSLYSPKSHSLFTVLASSPPKRRRSTTAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR NSSS RET+GKE SV ME+TE TSFGFNKRRAEG+DKTD PKKNLQLKVRKLNP 
Sbjct: 61  NFKRTNSSSFRETKGKENSVPMEETEATSFGFNKRRAEGRDKTDLPKKNLQLKVRKLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK-------- 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK        
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKVLHFEKSH 180

Query: 181 -------IDHIFLSRVCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFI 240
                  IDHIFLSRVCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFI
Sbjct: 181 PFSPEFQIDHIFLSRVCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFI 240

Query: 241 PNAAMVHTRSFGPALSSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDE 300
           PNAAMVHTRSFGP + SD DAV E SK  DPIVLVDDEVVKISAILV+PRPN G   FDE
Sbjct: 241 PNAAMVHTRSFGPTVRSDADAVDESSKCRDPIVLVDDEVVKISAILVYPRPNGGLGLFDE 300

Query: 301 DDMGRTQEERRNHDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKP 360
           DDMG +QE+RRNH+SEARSST  TSST+KPGDMSVVYVCELPEIKGKFDPSKAAA GLKP
Sbjct: 301 DDMGHSQEQRRNHNSEARSSTTNTSSTIKPGDMSVVYVCELPEIKGKFDPSKAAALGLKP 360

Query: 361 GPKYRELQLGNSVMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPY 420
           GPKYRELQLGNSVMSD QKIMVHPSDVLGPSVPGPVVLLVDCPTE+HL ELMSL+SLRPY
Sbjct: 361 GPKYRELQLGNSVMSDRQKIMVHPSDVLGPSVPGPVVLLVDCPTESHLSELMSLESLRPY 420

Query: 421 YEDFSSEQTETGKVVTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILR 480
           YEDFSS+QTE GKVVTCVIHLSPASILG+PNYQKWAKRFG AQHIMAGHDRKNVAIPILR
Sbjct: 421 YEDFSSDQTEIGKVVTCVIHLSPASILGNPNYQKWAKRFGSAQHIMAGHDRKNVAIPILR 480

Query: 481 SSAKIAARLNHLCPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTL 540
           +SAKIAARLNHLCPQLFPAPGFWSHQQLT PGSDSCA TEIEVSN++KSTLAENLLKFTL
Sbjct: 481 ASAKIAARLNHLCPQLFPAPGFWSHQQLTMPGSDSCALTEIEVSNYYKSTLAENLLKFTL 540

Query: 541 RPYAQLGLDRSSIPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNN 600
           RPYAQLGLDRS+IPSQESLPE ISALHS+IPEI+DAV HV QLWRGSAETDE+TP E+NN
Sbjct: 541 RPYAQLGLDRSNIPSQESLPEIISALHSEIPEIVDAVEHVGQLWRGSAETDERTPVEENN 600

Query: 601 AMVEEPWLDENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDC 660
           AMVEEPWL+ENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDC
Sbjct: 601 AMVEEPWLNENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDC 660

Query: 661 GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVL 720
           GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVL
Sbjct: 661 GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVL 720

Query: 721 VIGPRQLKRYLDAYQRLEDLDMQFLDCKHTTQASLEAFQNVAS--EIDKSPSESPISSTN 780
           VIGPRQLKRYL+AYQRLEDLDMQFLDCK TT+ASLEAFQNVAS  EID SPSESPISSTN
Sbjct: 721 VIGPRQLKRYLNAYQRLEDLDMQFLDCKDTTEASLEAFQNVASEIEIDYSPSESPISSTN 780

Query: 781 GSSTPIDRATGQKTTSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEAL 840
            +ST ID A  +KT +SLFVKGSRMQSYWKGPSSPVDIS A+PLLK LNEVLNEAGLEAL
Sbjct: 781 ENSTLIDGAIRRKTEASLFVKGSRMQSYWKGPSSPVDISTAVPLLKRLNEVLNEAGLEAL 840

Query: 841 ISFPVVHCPQAYGVVLKAAGR--------------------------------------A 900
           ISFPVVHCPQAYGVVLKAA R                                      A
Sbjct: 841 ISFPVVHCPQAYGVVLKAAERVNLDGKGIPGWKIVYSGDTRPCPKLMEASRGATLLIHEA 900

Query: 901 TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAF 947
           TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPV+ E+HMHKTCIAF
Sbjct: 901 TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVLDEKHMHKTCIAF 960

BLAST of HG10023368 vs. NCBI nr
Match: XP_022953731.1 (tRNase Z TRZ3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 829/987 (83.99%), Postives = 882/987 (89.36%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLFLSSFPALSPSK   FPS  SPKS S+FTV+A+S P+RRRS  AP S 
Sbjct: 1   MPLPHLSTLRFLFLSSFPALSPSK---FPSFCSPKSRSIFTVLAASSPRRRRS-GAPPSQ 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           +F R+N+SSLRE++GKEKSV ME+TE  SFGFNKRRAEG+DK D  KKNLQLKVR+LNP 
Sbjct: 61  SFNRRNNSSLRESKGKEKSVAMEETEAASFGFNKRRAEGRDKGDLAKKNLQLKVRRLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTI+YVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTIAYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGE GMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL
Sbjct: 181 VCSETAGGLPGLLLTLAGIGEAGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMG---RTQEERRN 300
           SSDVDA HELSKFCDPIVLVDDEVVKISAILVHP  NRGFQPF+EDDMG   R+QEERRN
Sbjct: 241 SSDVDATHELSKFCDPIVLVDDEVVKISAILVHPSSNRGFQPFNEDDMGQSNRSQEERRN 300

Query: 301 HDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNS 360
           HDSEARSST+K+SS VKPGDMSVVYVCELPEIKGKFDPSKAA+FGLKPGPKYRELQLGNS
Sbjct: 301 HDSEARSSTSKSSSAVKPGDMSVVYVCELPEIKGKFDPSKAASFGLKPGPKYRELQLGNS 360

Query: 361 VMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETG 420
           VMSD QKIMVHPSDVLGPSVPGPVVLLVDCPTEAH+LELMSLQSL+ YYED  S+QTETG
Sbjct: 361 VMSDRQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHVLELMSLQSLKTYYEDL-SDQTETG 420

Query: 421 KVVTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHL 480
           KVVTCVIHLSP SILGSPNYQKW KRFG +QHIMAGHDRKNV+IPIL+SSAKIAARLNHL
Sbjct: 421 KVVTCVIHLSPTSILGSPNYQKWVKRFGSSQHIMAGHDRKNVSIPILKSSAKIAARLNHL 480

Query: 481 CPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSS 540
           CPQLFPAPGFWS QQLT PGSDSCASTEIEVS+H+KSTLAENLLKFTLRPYAQLG DRSS
Sbjct: 481 CPQLFPAPGFWSPQQLTLPGSDSCASTEIEVSDHYKSTLAENLLKFTLRPYAQLGFDRSS 540

Query: 541 IPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENK 600
           IPSQESL E I+ LHSDIPEILDAVHHVSQLW GSA TD K   ED++AMVEEPWL+EN 
Sbjct: 541 IPSQESLSEVINGLHSDIPEILDAVHHVSQLWSGSAGTDVKIHVEDSHAMVEEPWLNENN 600

Query: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRY 660
           VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRY
Sbjct: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRY 660

Query: 661 GVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLD 720
           G+EGADAAVR LRCIWISHIHADHHTGLTRILALRRDLL E+PHEPVLVIGPRQLKRYLD
Sbjct: 661 GIEGADAAVRGLRCIWISHIHADHHTGLTRILALRRDLLREVPHEPVLVIGPRQLKRYLD 720

Query: 721 AYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKT 780
           AYQ LEDLDMQFLDCK+TTQASLEAFQ VA+EID S  E PISST+G++  ID A  +K 
Sbjct: 721 AYQILEDLDMQFLDCKYTTQASLEAFQKVATEIDNSSLEGPISSTDGNN--IDGA--KKP 780

Query: 781 TSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGV 840
            SSLFV+GSRMQSY KGPSSP+DI+AAMPLLK +N+VLNEAGLEALISFPVVHCPQ+YGV
Sbjct: 781 VSSLFVRGSRMQSYRKGPSSPIDINAAMPLLKYMNKVLNEAGLEALISFPVVHCPQSYGV 840

Query: 841 VLKAAGR--------------------------------------ATFEDSLVDEAMAKN 900
           +LKAA R                                      ATFEDSL+DEA+AKN
Sbjct: 841 ILKAAERVNLDGKVIPGWKIVYSGDTRPCPELMEASRGATVLIHEATFEDSLMDEAVAKN 900

Query: 901 HSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLP 947
           HSTTSEA+DIGNSAGAYRI+LTHFSQRYPKIPV+ ERHMHKTCIAFDLMSVNVADLLVLP
Sbjct: 901 HSTTSEAVDIGNSAGAYRIVLTHFSQRYPKIPVIEERHMHKTCIAFDLMSVNVADLLVLP 960

BLAST of HG10023368 vs. ExPASy Swiss-Prot
Match: F4J1H7 (tRNAse Z TRZ4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TRZ4 PE=2 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 2.3e-296
Identity = 570/979 (58.22%), Postives = 671/979 (68.54%), Query Frame = 0

Query: 15  SSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKR---RRSTAAPSSLNFKRKNSSSLR 74
           SS  AL    F  +P I++  SP+     +  PP+    RRS  +P      R+  SS  
Sbjct: 20  SSSFALILRPFSLYPPIFASSSPA----PSRRPPRTAGYRRSGPSP-----PRRKWSSFE 79

Query: 75  ETEGKEKSVTMEDT------EGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPANTISY 134
           E + K +S   +D          SF FNKRRAEG DK D PKKNL+   R LNP NTI+Y
Sbjct: 80  EQKRKGRSPMEKDKAISFNHSSDSFEFNKRRAEGLDKVDKPKKNLKRNTRTLNPTNTIAY 139

Query: 135 VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSRVCSET 194
           VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK+DHIFLSRVCSET
Sbjct: 140 VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKVDHIFLSRVCSET 199

Query: 195 AGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPALS-SDV 254
           AGGLPGLLLTLAGIGE G+SVNVWGPSDLKYLVDAM+SFIP AAMVHTRSFGP+L+ SD 
Sbjct: 200 AGGLPGLLLTLAGIGEQGLSVNVWGPSDLKYLVDAMRSFIPRAAMVHTRSFGPSLNISDS 259

Query: 255 DAVHELSK-FCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDSEAR 314
                LSK   D  VLVDDEVVKISAIL+ P               R +E          
Sbjct: 260 APQIGLSKPKDDAYVLVDDEVVKISAILLEP--------------SRLEE---------- 319

Query: 315 SSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMSDHQ 374
                  S  KPG+ +V+YVCELPEIKGKFDP KA A GL+ GPKY  LQ G SV SD +
Sbjct: 320 -------SGSKPGETAVIYVCELPEIKGKFDPKKAMALGLRAGPKYSYLQSGQSVKSDFK 379

Query: 375 KIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVVTCV 434
            I VHPSDV+GPSVPGPVVLLVDCPTE+H  EL+S+ S++ YY    +  T+  K+V C+
Sbjct: 380 DITVHPSDVMGPSVPGPVVLLVDCPTESHAEELLSIPSMKTYYSCLDN-STDGAKLVNCI 439

Query: 435 IHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQLFP 494
           IHLSPAS+  S  Y+ W KRF  AQHI+AGH+ KN+  PILR+S++I ARLN+LCPQ FP
Sbjct: 440 IHLSPASVTNSSTYRSWMKRFHSAQHILAGHEAKNMEFPILRASSRITARLNYLCPQFFP 499

Query: 495 APGFWSHQQLTFPGSDSCASTEIEV---SNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 554
           APGFWSHQ      ++S   T +     SN  +S  AENLLKFTLRP+  LG+DRSSIPS
Sbjct: 500 APGFWSHQH----DNNSINPTSLSKCFDSNLGESISAENLLKFTLRPHGNLGVDRSSIPS 559

Query: 555 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 614
           + +    +  L S+IPEI      + QLW G          + N  M+EEPWL E+ VPS
Sbjct: 560 RLTALRVMDELLSEIPEISSKTEEIKQLWNG----------QHNKMMIEEPWLGESTVPS 619

Query: 615 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 674
           CLENIRRDD+EIVLLGTGSSQPSKYRNV++IYI+LF +GS+LLDCGEGTLGQLKRRYG+E
Sbjct: 620 CLENIRRDDMEIVLLGTGSSQPSKYRNVTAIYIDLFSRGSILLDCGEGTLGQLKRRYGLE 679

Query: 675 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 734
           GAD AVR+LRCIWISHIHADHHTGL RILA RR+LL  + HEP +V+GPR LK +LDAYQ
Sbjct: 680 GADEAVRNLRCIWISHIHADHHTGLARILARRRELLKGLAHEPAIVVGPRSLKNFLDAYQ 739

Query: 735 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 794
           RLEDLDM+FLDC++TT  S        + ++ S  E   SS N                S
Sbjct: 740 RLEDLDMEFLDCRNTTTTSW-------ASVETSRPEKNTSSGNAE-------------GS 799

Query: 795 LFVKGSRMQSYWKGPSSPV-DISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVL 854
           LF KGS MQS +K PSSP+ D S+A+P LK L +VL E GLE LISFPVVHCPQA+GV L
Sbjct: 800 LFSKGSLMQSIYKRPSSPLTDNSSALPFLKKLKKVLGEMGLEHLISFPVVHCPQAFGVSL 859

Query: 855 KAAGR--------------------------------------ATFEDSLVDEAMAKNHS 914
           KAA R                                      ATFED+LV+EA+AKNHS
Sbjct: 860 KAAERKNIAGDEIPGWKMVYSGDTRPCPEMVEASKGATVLIHEATFEDALVEEAVAKNHS 919

Query: 915 TTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKV 941
           TT EAI +G+SAG YR +LTHFSQRYPKIPV+ E HMH TCIAFD+MS+N+ADL VLPK+
Sbjct: 920 TTKEAIKVGSSAGVYRTVLTHFSQRYPKIPVIDESHMHNTCIAFDMMSINMADLHVLPKI 923

BLAST of HG10023368 vs. ExPASy Swiss-Prot
Match: Q8VYS2 (tRNase Z TRZ3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=TRZ3 PE=1 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 9.5e-287
Identity = 554/986 (56.19%), Postives = 647/986 (65.62%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MP  H  +LR L L S  +   S FP     +SP+S SL T+ +SS              
Sbjct: 5   MPYLH-KNLRLLRLLSSKS---SPFPLSLRPFSPRSFSLSTLFSSS-------------- 64

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
                +SSS+   E    S     +   SF FNKRRAEG D TD  K+NL+ K +KLNP 
Sbjct: 65  ----SSSSSMENNEATNGS----KSSSNSFVFNKRRAEGFDITDKKKRNLERKSQKLNPT 124

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTI+Y QILGTGMDTQDTS SVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDH+FLSR
Sbjct: 125 NTIAYAQILGTGMDTQDTSSSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHVFLSR 184

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGE G+SVNVWGPSDL YLVDAMKSFIP AAMVHTRSFGP  
Sbjct: 185 VCSETAGGLPGLLLTLAGIGEEGLSVNVWGPSDLNYLVDAMKSFIPRAAMVHTRSFGP-- 244

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
                     S   DPIVLV+DEVVKISAI++ P          E+D G           
Sbjct: 245 ----------SSTPDPIVLVNDEVVKISAIILKP-------CHSEEDSGN---------- 304

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKA-AAFGLKPGPKYRELQLGNSVM 360
                        K GD+SVVYVCELPEI GKFD  KA   FG+KPGPKY  LQ G SV 
Sbjct: 305 -------------KSGDLSVVYVCELPEILGKFDLEKAKKVFGVKPGPKYSRLQSGESVK 364

Query: 361 SDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKV 420
           SD + I VHPSDV+GPS+PGP+VLLVDCPTE+H  EL SL+SL  YY     EQT   K 
Sbjct: 365 SDERDITVHPSDVMGPSLPGPIVLLVDCPTESHAAELFSLKSLESYYSS-PDEQTIGAKF 424

Query: 421 VTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCP 480
           V C+IHLSP+S+  SP YQ W K+F L QHI+AGH RKN+A PIL++S++IAARLN+LCP
Sbjct: 425 VNCIIHLSPSSVTSSPTYQSWMKKFHLTQHILAGHQRKNMAFPILKASSRIAARLNYLCP 484

Query: 481 QLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIP 540
           Q FPAPGFW  Q       D   S +   SN  +S  AENLLKF LRP A  G+DRS IP
Sbjct: 485 QFFPAPGFWPSQLTDNSIIDPTPSNKCSSSNLAESISAENLLKFNLRPVAIRGIDRSCIP 544

Query: 541 SQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDE--NK 600
           +  +  E +  L S+IPEI D    + Q W            + N  ++E+ WL E    
Sbjct: 545 APLTSSEVVDELLSEIPEIKDKSEEIKQFWN----------KQHNKTIIEKLWLSECNTV 604

Query: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRY 660
           +P+CLE IRRDD+EIV+LGTGSSQPSKYRNVS+I+I+LF +GS+LLDCGEGTLGQLKRRY
Sbjct: 605 LPNCLEKIRRDDMEIVILGTGSSQPSKYRNVSAIFIDLFSRGSLLLDCGEGTLGQLKRRY 664

Query: 661 GVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLD 720
           G++GAD AVR LRCIWISHIHADHHTGL RILALR  LL  + HEPV+V+GPR LKR+LD
Sbjct: 665 GLDGADEAVRKLRCIWISHIHADHHTGLARILALRSKLLKGVTHEPVIVVGPRPLKRFLD 724

Query: 721 AYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKT 780
           AYQRLEDLDM+FLDC+ TT  S  + +                            +G + 
Sbjct: 725 AYQRLEDLDMEFLDCRSTTATSWASLE----------------------------SGGEA 784

Query: 781 TSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGV 840
             SLF +GS MQS +K     +D S+ +  LK L +VL+E GL  LISFPVVHCPQAYGV
Sbjct: 785 EGSLFTQGSPMQSVFKRSDISMDNSSVLLCLKNLKKVLSEIGLNDLISFPVVHCPQAYGV 844

Query: 841 VLKAAGR--------------------------------------ATFEDSLVDEAMAKN 900
           V+KAA R                                      ATFED+L++EA+AKN
Sbjct: 845 VIKAAERVNSVGEQILGWKMVYSGDSRPCPETVEASRDATILIHEATFEDALIEEALAKN 883

Query: 901 HSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLP 946
           HSTT EAID+G++A  YRI+LTHFSQRYPKIPV+ E HMH TCIAFDLMS+N+ADL VLP
Sbjct: 905 HSTTKEAIDVGSAANVYRIVLTHFSQRYPKIPVIDESHMHNTCIAFDLMSINMADLHVLP 883

BLAST of HG10023368 vs. ExPASy Swiss-Prot
Match: Q8HY87 (Zinc phosphodiesterase ELAC protein 2 OS=Macaca fascicularis OX=9541 GN=ELAC2 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.4e-72
Identity = 266/899 (29.59%), Postives = 405/899 (45.05%), Query Frame = 0

Query: 68  SSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRK-----LNPANT 127
           S LR   G+    TM      S G  +R+   KD    P ++L+ + ++        ANT
Sbjct: 6   SLLRSATGR----TMSQGRTISQGSARRQRPPKD----PLRHLRTREKRGPSGSSGGANT 65

Query: 128 ISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSRVC 187
           + Y+Q++  G  ++D   ++ +F +  R++FN GEG+QR   EHK+K++++D+IFL+R+ 
Sbjct: 66  V-YLQVVAVG--SRDAGAALYVFSEFNRYLFNCGEGVQRLMQEHKLKVARLDNIFLTRMH 125

Query: 188 SETAGGLPGLLLTLAGIGEVGMSVNVW-GPSDLKYLVDAMKSFIPNAAMVHTRSFGPALS 247
               GGL G++LTL    E G+   V  GP  L+  ++A+K F            GP   
Sbjct: 126 WSNVGGLSGMILTLK---ETGLPKCVLSGPPQLEKYLEAIKIF-----------SGPLKG 185

Query: 248 SDVDA-VHELSKFCDPIVLVDDEVVKISAILVHPRPNRG-FQPFD--EDDMGRTQEERRN 307
            ++    H   ++        DE + +  I +H     G  QP+   E  +GR   E R+
Sbjct: 186 IELAVRPHSAPEY-------KDETMTVYQIPIHSEQRSGKHQPWQSPERPLGRLSPE-RS 245

Query: 308 HDSEARSST------AKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPG----- 367
            DSE+  S             V+   + V ++C+L   +G F   KA   GL  G     
Sbjct: 246 SDSESNESEPHLPRGVSQRRGVRDPSLVVAFICKLHLKRGSFLVLKAKELGLPVGTAAIA 305

Query: 368 PKYRELQLGNSVMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYY 427
           P    ++ G S+  + ++I+    ++  P  PG   ++V+CP E  +  +    + + Y 
Sbjct: 306 PIIAAVKDGKSITHEGREILA--EELCTPPDPGAAFVVVECPDEGFIQPICENATFQRY- 365

Query: 428 EDFSSEQTETGKVVTCVIHLSPASILGSPNYQKWAKRFGL-AQHIMAGHDRKNVAIPILR 487
                 Q +    V  V+H++P S+L    YQ+W +RFG   QH++     +N A     
Sbjct: 366 ------QGKADAPVALVVHMAPESVLADSRYQQWMERFGPDTQHLVL---NENCASVHNL 425

Query: 488 SSAKIAARLNHLCPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTL 547
            S KI  +LN + P +FP          +FP      +  + V         E LLK+ L
Sbjct: 426 RSYKIQTQLNLIHPDIFPL-------LTSFPRKKEGPTLSVPV------VQGECLLKYQL 485

Query: 548 RPYAQLGLDR-SSIPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDN 607
           RP  +   D   +   +E + E +      +P   +++    Q +R SA        +D 
Sbjct: 486 RPRREWQRDAIITCNPEEFIDEAL-----QLPNFQESM----QEYRRSA--------QDG 545

Query: 608 NAMVEEPWLDENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLD 667
            A  E+               R    EIV LGTGS+ P K RNVS+  +N+    S+LLD
Sbjct: 546 PAPAEK---------------RSQYPEIVFLGTGSAVPMKTRNVSATLVNISPDTSLLLD 605

Query: 668 CGEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEI--PHE 727
           CGEGT GQL R YG +  D  + SL  +++SH+HADHHTGL  IL  R   L  +  P  
Sbjct: 606 CGEGTFGQLYRHYG-DQVDRVLGSLAAVFVSHLHADHHTGLLNILLQRERALASLGKPFH 665

Query: 728 PVLVIGPRQLKRYLDAYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISST 787
           P+LV+ P QLK +L  Y        Q  +  H        +  V +EI     E  ISS 
Sbjct: 666 PLLVVAPTQLKAWLQQYHN------QCQEVLHHVSMIPAKYLQVGAEISSPAVERLISS- 725

Query: 788 NGSSTPIDRATGQKTTSSLFVKGSR--------MQSYWKGPSSPVDISAAMPLLKCLNEV 847
                 + R    +   +  V+  R          S WK     V     MP      E 
Sbjct: 726 ------LLRTCDLEEFQTCLVRHCRHAFGCALVHTSGWK----VVYSGDTMPC-----EA 775

Query: 848 LNEAGLEALISFPVVHCPQAYGVVLKAAGRATFEDSLVDEAMAKNHSTTSEAIDIGNSAG 907
           L + G +A +   ++H              AT ED L +EA+ K HSTTS+AI +G    
Sbjct: 786 LVQMGKDATL---LIH-------------EATLEDGLEEEAVEKTHSTTSQAIRVGMRMN 775

Query: 908 AYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVLPYLKLLFRDEM 934
           A  I+L HFSQRY K+P+ S     K  IAFD M V+  D   +PK++P LK LF  ++
Sbjct: 846 AEFIMLNHFSQRYAKVPLFSPDFNEKVGIAFDHMKVSFGDFPTVPKLIPPLKALFAGDI 775

BLAST of HG10023368 vs. ExPASy Swiss-Prot
Match: Q9GL72 (Zinc phosphodiesterase ELAC protein 2 OS=Pan troglodytes OX=9598 GN=ELAC2 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 5.0e-70
Identity = 245/837 (29.27%), Postives = 377/837 (45.04%), Query Frame = 0

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NT+ Y+Q++  G  ++D+  ++ +F +  R++FN GEG+QR   EHK+K++++D+IFL+R
Sbjct: 56  NTV-YLQVVAAG--SRDSGAALYVFSEFNRYLFNCGEGIQRLMQEHKLKVARLDNIFLTR 115

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVW-GPSDLKYLVDAMKSFIPNAAMVHTRSFGPA 240
           +     GGL G++LTL    E G+   V  GP  L+  ++A+K F            GP 
Sbjct: 116 MHWSNVGGLSGMILTLK---ETGLPKCVLSGPPQLEKYLEAIKIF-----------SGPL 175

Query: 241 LSSDVDA-VHELSKFCDPIVLVDDEVVKISAILVHPRPNRG-FQPFD--EDDMGRTQEER 300
              ++    H   ++       +DE + +  I +H    RG  QP+   E  + R   E 
Sbjct: 176 KGIELAVRPHSAPEY-------EDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPE- 235

Query: 301 RNHDSEARSST------AKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPG--- 360
           R+ DSE+  +             V+   + V ++C+L   +G F   KA   GL  G   
Sbjct: 236 RSSDSESNENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAA 295

Query: 361 --PKYRELQLGNSVMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRP 420
             P    ++ G S+  + ++I+    ++  P  PG   ++V+CP E+ +  +    + + 
Sbjct: 296 IAPIIAAVKDGKSITHEGREILA--EELCTPPDPGAAFVVVECPDESFIQPICENATFQR 355

Query: 421 YYEDFSSEQTETGKVVTCVIHLSPASILGSPNYQKWAKRFGL-AQHIMAGHDRKNVAIPI 480
           Y       Q +    V  V+H++P S+L    YQ+W +RFG   QH++     +N A   
Sbjct: 356 Y-------QGKADAPVALVVHMAPESVLVDSRYQQWMERFGPDTQHLVL---NENCASVH 415

Query: 481 LRSSAKIAARLNHLCPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKF 540
              S KI  +LN + P +FP          +FP      +  + +         E LLK+
Sbjct: 416 NLRSHKIQTQLNLIHPDIFPL-------LTSFPCKKEGPTLSVPM------VQGECLLKY 475

Query: 541 TLRPYAQLGLDRSSIPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNED 600
            LRP  +   D     + E     I AL   +P    +V    Q +R SA        +D
Sbjct: 476 QLRPRREWQRDAIITCNPEEF--IIEAL--QLPNFQQSV----QEYRRSA--------QD 535

Query: 601 NNAMVEEPWLDENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLL 660
             A  E+               R    EI+ LGTGS+ P K RNVS+  +N+    S+LL
Sbjct: 536 GPAPAEK---------------RSQYPEIIFLGTGSAIPMKIRNVSATLVNISPDTSLLL 595

Query: 661 DCGEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEI--PH 720
           DCGEGT GQL R YG +  D  + +L  +++SH+HADHHTGL  IL  R   L  +  P 
Sbjct: 596 DCGEGTFGQLCRHYG-DQVDRVLGTLAAVFVSHLHADHHTGLLNILLQRERALASLGKPF 655

Query: 721 EPVLVIGPRQLKRYLDAYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISS 780
            P+LV+ P QLK +L  Y        Q  +  H             +EI     E  ISS
Sbjct: 656 HPLLVVAPNQLKAWLQQYHN------QCQEVLHHISMIPAKCLQEGAEISSPAVERLISS 715

Query: 781 TNGSSTPIDRAT-----GQKTTSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLN 840
              +    +  T      +       V  S  +  + G + P              E L 
Sbjct: 716 LLRTCDLEEFQTCLVRHCKHAFGCALVHTSGWKVVYSGDTMPC-------------EALV 775

Query: 841 EAGLEALISFPVVHCPQAYGVVLKAAGRATFEDSLVDEAMAKNHSTTSEAIDIGNSAGAY 900
             G +A +   ++H              AT ED L +EA+ K HSTTS+AI +G    A 
Sbjct: 776 RMGKDATL---LIH-------------EATLEDGLEEEAVEKTHSTTSQAISVGMRMNAE 775

Query: 901 RIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVLPYLKLLFRDEM 934
            I+L HFSQRY K+P+ S     K  +AFD M V   D   +PK++P LK LF  ++
Sbjct: 836 FIMLNHFSQRYAKVPLFSPNFNEKVGVAFDHMKVCFGDFATMPKLIPPLKALFAGDI 775

BLAST of HG10023368 vs. ExPASy Swiss-Prot
Match: Q80Y81 (Zinc phosphodiesterase ELAC protein 2 OS=Mus musculus OX=10090 GN=Elac2 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.5e-69
Identity = 248/843 (29.42%), Postives = 382/843 (45.31%), Query Frame = 0

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NT+ Y+Q++  G   +D   ++ +F +  R++FN GEG+QR   EHK+K++++D+IFL+R
Sbjct: 48  NTV-YLQVVAAG--GRDAGAALYVFSEYNRYLFNCGEGVQRLMQEHKLKVARLDNIFLTR 107

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVW-GPSDLKYLVDAMKSFIPNAAMVHTRSFGPA 240
           +     GGL G++LTL    E G+   V  GP  L+  ++A+K F            GP 
Sbjct: 108 MHWSNVGGLCGMILTLK---ETGLPKCVLSGPPQLEKYLEAIKIF-----------SGPL 167

Query: 241 LSSDVDA-VHELSKFCDPIVLVDDEVVKISAILVH-------------PR--PNRGFQPF 300
              ++    H   ++        DE + +  + +H             PR  PNR   P 
Sbjct: 168 KGIELAVRPHSAPEY-------KDETMTVYQVPIHSERRCGKQQPSQSPRTSPNR-LSPK 227

Query: 301 DEDDMGRTQEERRNHDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGL 360
              D G    E      E  S+ A   +  +   + V +VC+L   KG F   KA   GL
Sbjct: 228 QSSDSG--SAENGQCPPEDSSAGANRKAWGRDPSLVVAFVCKLHLRKGNFLVLKAKELGL 287

Query: 361 KPG-----PKYRELQLGNSVMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMS 420
             G     P    ++ G S+  + ++I     ++  P  PG V ++V+CP E  +L +  
Sbjct: 288 PVGTAAIAPIIAAVKDGKSITYEGREIAA--EELCTPPDPGLVFIVVECPDEGFILPICE 347

Query: 421 LQSLRPYYEDFSSEQTETGKVVTCVIHLSPASILGSPNYQKWAKRFGL-AQHIMAGHDRK 480
             + + Y       Q E    V  V+H++P S+L    YQ+W +RFG   QH++   +  
Sbjct: 348 NDTFKRY-------QAEADAPVALVVHIAPESVLIDSRYQQWMERFGPDTQHLILNENCP 407

Query: 481 NVAIPILRSSAKIAARLNHLCPQLFP-APGFWSHQQLTFPGSDSCASTEIEVSNHHKSTL 540
           +V    LRS  KI  +L+ + P +FP    F+S ++    GS     T            
Sbjct: 408 SV--HNLRSH-KIQTQLSLIHPDIFPQLTSFYSKEE----GSTLSVPT----------VR 467

Query: 541 AENLLKFTLRPYAQLGLDRS-SIPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAET 600
            E LLK+ LRP  +   D +    + E + E +     ++P   ++V             
Sbjct: 468 GECLLKYQLRPKREWQRDTTLDCNTDEFIAEAL-----ELPSFQESV------------- 527

Query: 601 DEKTPNEDNNAMVEEPWLDENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINL 660
           +E   N           + EN  P+     R    EIV LGTGS+ P K RNVSS  +NL
Sbjct: 528 EEYRKN-----------VQENPAPA---EKRSQYPEIVFLGTGSAIPMKIRNVSSTLVNL 587

Query: 661 FLKGSMLLDCGEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDL 720
               S+LLDCGEGT GQL R YG +  D  + SL  +++SH+HADHHTGL  IL  R   
Sbjct: 588 SPDKSVLLDCGEGTFGQLCRHYG-QQIDRVLCSLTAVFVSHLHADHHTGLLNILLQREHA 647

Query: 721 LWEI--PHEPVLVIGPRQLKRYLDAYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKS 780
           L  +  P +P+LV+ P QL+ +L  Y            C           Q +   +   
Sbjct: 648 LASLGKPFQPLLVVAPTQLRAWLQQYHN---------HC-----------QEILHHVSMI 707

Query: 781 PSESPISSTNGSSTPIDRATGQKTTSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNE 840
           P++        S+T ++R        SL ++   ++ +         +        C   
Sbjct: 708 PAKCLQKGAEVSNTTLERLI------SLLLETCDLEEF-----QTCLVRHCKHAFGC--A 767

Query: 841 VLNEAGLEALISFPVVHCPQAYGVVLKAA---GRATFEDSLVDEAMAKNHSTTSEAIDIG 900
           +++ +G + + S   + C     +   A      AT ED L +EA+ K HSTTS+AI++G
Sbjct: 768 LVHSSGWKVVYSGDTMPCEALVQMGKDATLLIHEATLEDGLEEEAVEKTHSTTSQAINVG 771

Query: 901 NSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVLPYLKLLFR 934
               A  I+L HFSQRY KIP+ S     K  IAFD M V   D   +PK++P LK LF 
Sbjct: 828 MRMNAEFIMLNHFSQRYAKIPLFSPDFNEKVGIAFDHMKVCFGDFPTVPKLIPPLKALFA 771

BLAST of HG10023368 vs. ExPASy TrEMBL
Match: A0A0A0KAI6 (Ribonuclease Z OS=Cucumis sativus OX=3659 GN=Csa_7G447120 PE=3 SV=1)

HSP 1 Score: 1656.0 bits (4287), Expect = 0.0e+00
Identity = 851/984 (86.48%), Postives = 890/984 (90.45%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLF       SPSK PF PS+YSPKS SLFTV+ASSPPKRRRS  AP SL
Sbjct: 1   MPLPHLSTLRFLF------FSPSKLPFSPSLYSPKSHSLFTVLASSPPKRRRSATAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR+NSSSLRET+GKE SV ME+TE TSFGFNKRRAEG+DKTD PKKNLQLKVRKLNPA
Sbjct: 61  NFKRRNSSSLRETKGKENSVPMEETEATSFGFNKRRAEGRDKTDLPKKNLQLKVRKLNPA 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGP +
Sbjct: 181 VCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPTV 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
           SSD DAVHELSK  +PIVLVDDEVVKISAILVHPRPNR    FDEDDMG +QE+RRN +S
Sbjct: 241 SSDADAVHELSKCREPIVLVDDEVVKISAILVHPRPNRRLGLFDEDDMGHSQEQRRNDNS 300

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360
           EARSST KTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAA GLKPGPKYRELQLGNSVMS
Sbjct: 301 EARSSTTKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAALGLKPGPKYRELQLGNSVMS 360

Query: 361 DHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVV 420
           DHQKIMVHPSDVLGPSVPGPVVLL+DCPTE+HL ELMSL+SLRPYYED SS+QTETGKVV
Sbjct: 361 DHQKIMVHPSDVLGPSVPGPVVLLIDCPTESHLSELMSLESLRPYYEDLSSDQTETGKVV 420

Query: 421 TCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480
           TCVIHLSPASILG+PNYQKWA+RF  AQHIMAGH RKNVAIPILR+SAKIAARLNHLCPQ
Sbjct: 421 TCVIHLSPASILGNPNYQKWARRFESAQHIMAGHHRKNVAIPILRASAKIAARLNHLCPQ 480

Query: 481 LFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 540
           LFPAPGFWSHQQLT PGSDSCASTEIEVSNH+KSTLAENLLKFTLRPYAQLG DRS+IPS
Sbjct: 481 LFPAPGFWSHQQLTMPGSDSCASTEIEVSNHYKSTLAENLLKFTLRPYAQLGFDRSNIPS 540

Query: 541 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 600
           QESLPE I+ALHS+IPEI+DAV HVSQLWRGSAETDE+TP E+NNAMVEEPWLDENKVPS
Sbjct: 541 QESLPEIINALHSEIPEIVDAVEHVSQLWRGSAETDERTPVEENNAMVEEPWLDENKVPS 600

Query: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 660
           CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRYGVE
Sbjct: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRYGVE 660

Query: 661 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 720
           GADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVLVIGPRQL+RYL+AYQ
Sbjct: 661 GADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVLVIGPRQLRRYLNAYQ 720

Query: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 780
           RLEDLDMQFLDCK TT+ASLEAFQ +AS+ID SPSESPISSTN +ST ID   G+KT SS
Sbjct: 721 RLEDLDMQFLDCKDTTEASLEAFQKLASDIDNSPSESPISSTNENSTLIDGTIGRKTESS 780

Query: 781 LFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840
           LFVKGSRMQSYWKGPSSPVDI+AA+PLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK
Sbjct: 781 LFVKGSRMQSYWKGPSSPVDINAAVPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840

Query: 841 AAGR--------------------------------------ATFEDSLVDEAMAKNHST 900
           AA R                                      ATFEDSLVDEAMAKNHST
Sbjct: 841 AAERVNLDGKVIPGWKIVYSGDTRPCPKLMEASRGATLLIHEATFEDSLVDEAMAKNHST 900

Query: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVL 947
           TSEAIDIGNSAGAYRIILTHFSQRYPKIPVV E+HMHKTCIAFDLMSVNVADL VLPKVL
Sbjct: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVDEKHMHKTCIAFDLMSVNVADLSVLPKVL 960

BLAST of HG10023368 vs. ExPASy TrEMBL
Match: A0A1S3CHV9 (Ribonuclease Z OS=Cucumis melo OX=3656 GN=LOC103500604 PE=3 SV=1)

HSP 1 Score: 1652.9 bits (4279), Expect = 0.0e+00
Identity = 850/984 (86.38%), Postives = 885/984 (89.94%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPH S+LRFLF       SPSK PFFPS+YSPKS SLFTV+ASSPPKRRRST AP SL
Sbjct: 1   MPLPHPSTLRFLF------FSPSKLPFFPSLYSPKSHSLFTVLASSPPKRRRSTTAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR NSSS RET+GKE SV ME+TEGTSFGFNK+RAEG+DKTD PKKNLQLKVRKLNP 
Sbjct: 61  NFKRTNSSSFRETKGKENSVPMEETEGTSFGFNKKRAEGRDKTDLPKKNLQLKVRKLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGP +
Sbjct: 181 VCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPTV 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
            SD DAV ELSK  DPIVLVDDEVVKISAILVHPRPN G   FDEDDMG +QE+RRNH+S
Sbjct: 241 HSDADAVDELSKCRDPIVLVDDEVVKISAILVHPRPNGGLGLFDEDDMGHSQEQRRNHNS 300

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMS 360
           EARS T  TSST+KPGDMSVVYVCELPEIKGKFDPSKAAA GLKPGPKYRELQLGNSVMS
Sbjct: 301 EARSLTTNTSSTIKPGDMSVVYVCELPEIKGKFDPSKAAALGLKPGPKYRELQLGNSVMS 360

Query: 361 DHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVV 420
           D QKIMVHPSDVLGPSVPGPVVLLVDCPTE+HL ELMSL+SLRPYYEDFSS+QTE GKVV
Sbjct: 361 DRQKIMVHPSDVLGPSVPGPVVLLVDCPTESHLSELMSLESLRPYYEDFSSDQTEIGKVV 420

Query: 421 TCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQ 480
           TCVIHLSPASILG+PNYQKWAKRFG AQHIMAGHDRKNVAIPILR+SAKIAARLNHLCPQ
Sbjct: 421 TCVIHLSPASILGNPNYQKWAKRFGSAQHIMAGHDRKNVAIPILRASAKIAARLNHLCPQ 480

Query: 481 LFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 540
           LFPAPGFWSHQQLT PGSDSCA TEIEVSNH+KSTLAENLLKFTLRPYAQLGLDRS+IPS
Sbjct: 481 LFPAPGFWSHQQLTMPGSDSCALTEIEVSNHYKSTLAENLLKFTLRPYAQLGLDRSNIPS 540

Query: 541 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 600
           QESLPE ISALHS+IPEI+DAV HV QLWRGSAETDE+TP E+NNAMVEEPWL+ENKVPS
Sbjct: 541 QESLPEIISALHSEIPEIVDAVEHVGQLWRGSAETDERTPVEENNAMVEEPWLNENKVPS 600

Query: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 660
           CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRYGVE
Sbjct: 601 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRYGVE 660

Query: 661 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 720
           GADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVLVIGPRQLKRYL+AYQ
Sbjct: 661 GADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVLVIGPRQLKRYLNAYQ 720

Query: 721 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 780
           RLEDLDMQFLDCK TT+ASLEAFQNVASEID SPSESPISSTN +ST ID A  +KT +S
Sbjct: 721 RLEDLDMQFLDCKDTTEASLEAFQNVASEIDYSPSESPISSTNENSTLIDGAIRRKTEAS 780

Query: 781 LFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840
           LFVKGSRMQSYWKGPSSPVDIS A+PLLK LNEVLNEAGLEALISFPVVHCPQAYGVVLK
Sbjct: 781 LFVKGSRMQSYWKGPSSPVDISTAVPLLKRLNEVLNEAGLEALISFPVVHCPQAYGVVLK 840

Query: 841 AAGR--------------------------------------ATFEDSLVDEAMAKNHST 900
           AA R                                      ATFEDSLVDEAMAKNHST
Sbjct: 841 AAERVNLDGKGIPGWKIVYSGDTRPCPKLMEASRGATLLIHEATFEDSLVDEAMAKNHST 900

Query: 901 TSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKVL 947
           TSEAIDIGNS GAYRIILTHFSQRYPKIPV+ E+HMHKTCIAFDLMSVNVADL VLPKVL
Sbjct: 901 TSEAIDIGNSVGAYRIILTHFSQRYPKIPVLDEKHMHKTCIAFDLMSVNVADLSVLPKVL 960

BLAST of HG10023368 vs. ExPASy TrEMBL
Match: A0A5D3C0N0 (Ribonuclease Z OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002040 PE=3 SV=1)

HSP 1 Score: 1636.3 bits (4236), Expect = 0.0e+00
Identity = 850/1001 (84.92%), Postives = 886/1001 (88.51%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLF       SPSK PFFPS+YSPKS SLFTV+ASSPPKRRRST AP SL
Sbjct: 1   MPLPHLSTLRFLF------FSPSKLPFFPSLYSPKSHSLFTVLASSPPKRRRSTTAPPSL 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           NFKR NSSS RET+GKE SV ME+TE TSFGFNKRRAEG+DKTD PKKNLQLKVRKLNP 
Sbjct: 61  NFKRTNSSSFRETKGKENSVPMEETEATSFGFNKRRAEGRDKTDLPKKNLQLKVRKLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK-------- 180
           NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK        
Sbjct: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKVLHFEKSH 180

Query: 181 -------IDHIFLSRVCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFI 240
                  IDHIFLSRVCSETAGGLPGLLLTLAGIG+VGMSVNVWGPSDLKYLVDAMKSFI
Sbjct: 181 PFSPEFQIDHIFLSRVCSETAGGLPGLLLTLAGIGDVGMSVNVWGPSDLKYLVDAMKSFI 240

Query: 241 PNAAMVHTRSFGPALSSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDE 300
           PNAAMVHTRSFGP + SD DAV E SK  DPIVLVDDEVVKISAILV+PRPN G   FDE
Sbjct: 241 PNAAMVHTRSFGPTVRSDADAVDESSKCRDPIVLVDDEVVKISAILVYPRPNGGLGLFDE 300

Query: 301 DDMGRTQEERRNHDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKP 360
           DDMG +QE+RRNH+SEARSST  TSST+KPGDMSVVYVCELPEIKGKFDPSKAAA GLKP
Sbjct: 301 DDMGHSQEQRRNHNSEARSSTTNTSSTIKPGDMSVVYVCELPEIKGKFDPSKAAALGLKP 360

Query: 361 GPKYRELQLGNSVMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPY 420
           GPKYRELQLGNSVMSD QKIMVHPSDVLGPSVPGPVVLLVDCPTE+HL ELMSL+SLRPY
Sbjct: 361 GPKYRELQLGNSVMSDRQKIMVHPSDVLGPSVPGPVVLLVDCPTESHLSELMSLESLRPY 420

Query: 421 YEDFSSEQTETGKVVTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILR 480
           YEDFSS+QTE GKVVTCVIHLSPASILG+PNYQKWAKRFG AQHIMAGHDRKNVAIPILR
Sbjct: 421 YEDFSSDQTEIGKVVTCVIHLSPASILGNPNYQKWAKRFGSAQHIMAGHDRKNVAIPILR 480

Query: 481 SSAKIAARLNHLCPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTL 540
           +SAKIAARLNHLCPQLFPAPGFWSHQQLT PGSDSCA TEIEVSN++KSTLAENLLKFTL
Sbjct: 481 ASAKIAARLNHLCPQLFPAPGFWSHQQLTMPGSDSCALTEIEVSNYYKSTLAENLLKFTL 540

Query: 541 RPYAQLGLDRSSIPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNN 600
           RPYAQLGLDRS+IPSQESLPE ISALHS+IPEI+DAV HV QLWRGSAETDE+TP E+NN
Sbjct: 541 RPYAQLGLDRSNIPSQESLPEIISALHSEIPEIVDAVEHVGQLWRGSAETDERTPVEENN 600

Query: 601 AMVEEPWLDENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDC 660
           AMVEEPWL+ENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDC
Sbjct: 601 AMVEEPWLNENKVPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDC 660

Query: 661 GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVL 720
           GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGL RILALRRDLL E+PHEPVL
Sbjct: 661 GEGTLGQLKRRYGVEGADAAVRSLRCIWISHIHADHHTGLARILALRRDLLREVPHEPVL 720

Query: 721 VIGPRQLKRYLDAYQRLEDLDMQFLDCKHTTQASLEAFQNVAS--EIDKSPSESPISSTN 780
           VIGPRQLKRYL+AYQRLEDLDMQFLDCK TT+ASLEAFQNVAS  EID SPSESPISSTN
Sbjct: 721 VIGPRQLKRYLNAYQRLEDLDMQFLDCKDTTEASLEAFQNVASEIEIDYSPSESPISSTN 780

Query: 781 GSSTPIDRATGQKTTSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEAL 840
            +ST ID A  +KT +SLFVKGSRMQSYWKGPSSPVDIS A+PLLK LNEVLNEAGLEAL
Sbjct: 781 ENSTLIDGAIRRKTEASLFVKGSRMQSYWKGPSSPVDISTAVPLLKRLNEVLNEAGLEAL 840

Query: 841 ISFPVVHCPQAYGVVLKAAGR--------------------------------------A 900
           ISFPVVHCPQAYGVVLKAA R                                      A
Sbjct: 841 ISFPVVHCPQAYGVVLKAAERVNLDGKGIPGWKIVYSGDTRPCPKLMEASRGATLLIHEA 900

Query: 901 TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAF 947
           TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPV+ E+HMHKTCIAF
Sbjct: 901 TFEDSLVDEAMAKNHSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVLDEKHMHKTCIAF 960

BLAST of HG10023368 vs. ExPASy TrEMBL
Match: A0A6J1GNW7 (Ribonuclease Z OS=Cucurbita moschata OX=3662 GN=LOC111456177 PE=3 SV=1)

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 829/987 (83.99%), Postives = 882/987 (89.36%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLFLSSFPALSPSK   FPS  SPKS S+FTV+A+S P+RRRS  AP S 
Sbjct: 1   MPLPHLSTLRFLFLSSFPALSPSK---FPSFCSPKSRSIFTVLAASSPRRRRS-GAPPSQ 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           +F R+N+SSLRE++GKEKSV ME+TE  SFGFNKRRAEG+DK D  KKNLQLKVR+LNP 
Sbjct: 61  SFNRRNNSSLRESKGKEKSVAMEETEAASFGFNKRRAEGRDKGDLAKKNLQLKVRRLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTI+YVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTIAYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGE GMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL
Sbjct: 181 VCSETAGGLPGLLLTLAGIGEAGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMG---RTQEERRN 300
           SSDVDA HELSKFCDPIVLVDDEVVKISAILVHP  NRGFQPF+EDDMG   R+QEERRN
Sbjct: 241 SSDVDATHELSKFCDPIVLVDDEVVKISAILVHPSSNRGFQPFNEDDMGQSNRSQEERRN 300

Query: 301 HDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNS 360
           HDSEARSST+K+SS VKPGDMSVVYVCELPEIKGKFDPSKAA+FGLKPGPKYRELQLGNS
Sbjct: 301 HDSEARSSTSKSSSAVKPGDMSVVYVCELPEIKGKFDPSKAASFGLKPGPKYRELQLGNS 360

Query: 361 VMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETG 420
           VMSD QKIMVHPSDVLGPSVPGPVVLLVDCPTEAH+LELMSLQSL+ YYED  S+QTETG
Sbjct: 361 VMSDRQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHVLELMSLQSLKTYYEDL-SDQTETG 420

Query: 421 KVVTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHL 480
           KVVTCVIHLSP SILGSPNYQKW KRFG +QHIMAGHDRKNV+IPIL+SSAKIAARLNHL
Sbjct: 421 KVVTCVIHLSPTSILGSPNYQKWVKRFGSSQHIMAGHDRKNVSIPILKSSAKIAARLNHL 480

Query: 481 CPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSS 540
           CPQLFPAPGFWS QQLT PGSDSCASTEIEVS+H+KSTLAENLLKFTLRPYAQLG DRSS
Sbjct: 481 CPQLFPAPGFWSPQQLTLPGSDSCASTEIEVSDHYKSTLAENLLKFTLRPYAQLGFDRSS 540

Query: 541 IPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENK 600
           IPSQESL E I+ LHSDIPEILDAVHHVSQLW GSA TD K   ED++AMVEEPWL+EN 
Sbjct: 541 IPSQESLSEVINGLHSDIPEILDAVHHVSQLWSGSAGTDVKIHVEDSHAMVEEPWLNENN 600

Query: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRY 660
           VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRY
Sbjct: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRY 660

Query: 661 GVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLD 720
           G+EGADAAVR LRCIWISHIHADHHTGLTRILALRRDLL E+PHEPVLVIGPRQLKRYLD
Sbjct: 661 GIEGADAAVRGLRCIWISHIHADHHTGLTRILALRRDLLREVPHEPVLVIGPRQLKRYLD 720

Query: 721 AYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKT 780
           AYQ LEDLDMQFLDCK+TTQASLEAFQ VA+EID S  E PISST+G++  ID A  +K 
Sbjct: 721 AYQILEDLDMQFLDCKYTTQASLEAFQKVATEIDNSSLEGPISSTDGNN--IDGA--KKP 780

Query: 781 TSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGV 840
            SSLFV+GSRMQSY KGPSSP+DI+AAMPLLK +N+VLNEAGLEALISFPVVHCPQ+YGV
Sbjct: 781 VSSLFVRGSRMQSYRKGPSSPIDINAAMPLLKYMNKVLNEAGLEALISFPVVHCPQSYGV 840

Query: 841 VLKAAGR--------------------------------------ATFEDSLVDEAMAKN 900
           +LKAA R                                      ATFEDSL+DEA+AKN
Sbjct: 841 ILKAAERVNLDGKVIPGWKIVYSGDTRPCPELMEASRGATVLIHEATFEDSLMDEAVAKN 900

Query: 901 HSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLP 947
           HSTTSEA+DIGNSAGAYRI+LTHFSQRYPKIPV+ ERHMHKTCIAFDLMSVNVADLLVLP
Sbjct: 901 HSTTSEAVDIGNSAGAYRIVLTHFSQRYPKIPVIEERHMHKTCIAFDLMSVNVADLLVLP 960

BLAST of HG10023368 vs. ExPASy TrEMBL
Match: A0A6J1JY33 (Ribonuclease Z OS=Cucurbita maxima OX=3661 GN=LOC111488537 PE=3 SV=1)

HSP 1 Score: 1591.6 bits (4120), Expect = 0.0e+00
Identity = 822/987 (83.28%), Postives = 876/987 (88.75%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MPLPHLS+LRFLFLSSFPALSPSK   FPS  SPKS S+FTV+A+S PKRRRS  AP S 
Sbjct: 1   MPLPHLSTLRFLFLSSFPALSPSK---FPSFCSPKSRSIFTVLAASSPKRRRS-GAPPSQ 60

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
           +F R+N+SSLRE +GKEKS  ME+ E  SFGFNKRRAEG+DK D  KKNLQLKVR+LNP 
Sbjct: 61  SFNRRNNSSLREIKGKEKSAAMEEAEAASFGFNKRRAEGRDKGDLAKKNLQLKVRRLNPV 120

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTI+YVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR
Sbjct: 121 NTIAYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGE GMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL
Sbjct: 181 VCSETAGGLPGLLLTLAGIGEAGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMG---RTQEERRN 300
           SSD+DA HELSKFCDPIVLVDDEVVKISAILVHP  NRGFQPF+EDDMG   R+QEERRN
Sbjct: 241 SSDIDAAHELSKFCDPIVLVDDEVVKISAILVHPSSNRGFQPFNEDDMGQSNRSQEERRN 300

Query: 301 HDSEARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNS 360
           HDSEARSST+K+SS VKPGDMSVVYVCELPEIKGKFDPSKAA+FGLKPGPKYRELQLGNS
Sbjct: 301 HDSEARSSTSKSSSAVKPGDMSVVYVCELPEIKGKFDPSKAASFGLKPGPKYRELQLGNS 360

Query: 361 VMSDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETG 420
           VMSD Q IMVHPSDVLGPSVPGPVVLLVDCPTEAH+LELMSLQSL+ YYED  S+Q ETG
Sbjct: 361 VMSDRQNIMVHPSDVLGPSVPGPVVLLVDCPTEAHVLELMSLQSLKTYYEDL-SDQAETG 420

Query: 421 KVVTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHL 480
           KVVTCVIHLSP SILGSPNYQKW KRFG +QHIMAGHDRKNV+IPIL+SSAKIAARLNHL
Sbjct: 421 KVVTCVIHLSPTSILGSPNYQKWVKRFGSSQHIMAGHDRKNVSIPILKSSAKIAARLNHL 480

Query: 481 CPQLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSS 540
           CPQLFPAPGFWS QQLT PGSDSCASTEIEVS+H+KSTLAENLLKFTLRPYAQLG DRSS
Sbjct: 481 CPQLFPAPGFWSPQQLTLPGSDSCASTEIEVSDHYKSTLAENLLKFTLRPYAQLGFDRSS 540

Query: 541 IPSQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENK 600
           IPSQESL E I+ LHSDIPEILDAVHHV+QLW GSA TD     ED++ MVEEPW +EN 
Sbjct: 541 IPSQESLSEVINGLHSDIPEILDAVHHVTQLWSGSAGTDTNIHVEDSHTMVEEPWFNENN 600

Query: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRY 660
           +PSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLF KGSMLLDCGEGTLGQLKRRY
Sbjct: 601 IPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFSKGSMLLDCGEGTLGQLKRRY 660

Query: 661 GVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLD 720
           G+EGADAAVR LRCIWISHIHADHHTGLTRILALRRDLL E+PHEPVLVIGPRQLKRYLD
Sbjct: 661 GIEGADAAVRGLRCIWISHIHADHHTGLTRILALRRDLLREVPHEPVLVIGPRQLKRYLD 720

Query: 721 AYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKT 780
           AYQ LEDLDMQFLDCK+TTQASLEAFQ VA+EID SP E PISST+G++  ID   G+KT
Sbjct: 721 AYQILEDLDMQFLDCKYTTQASLEAFQKVATEIDNSPLEGPISSTDGNN--ID--GGKKT 780

Query: 781 TSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGV 840
            SSLFV+GSRMQSY KGPSSP+DI+AAMPLLK +N+VLNEAGLEALISFPVVHCPQ+YGV
Sbjct: 781 MSSLFVRGSRMQSYRKGPSSPIDINAAMPLLKNMNKVLNEAGLEALISFPVVHCPQSYGV 840

Query: 841 VLKAAGR--------------------------------------ATFEDSLVDEAMAKN 900
           +LKAA R                                      ATFEDSL+DEA+AKN
Sbjct: 841 ILKAAERVNLDGKVIPGWKIVYSGDTRPCPELMEASRGATVLIHEATFEDSLMDEAVAKN 900

Query: 901 HSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLP 947
           HSTTSEA+DIGNSAGAYRI+LTHFSQRYPKIPV+ ERHMHKTCIAFDLMSVNVADLLVLP
Sbjct: 901 HSTTSEAVDIGNSAGAYRIVLTHFSQRYPKIPVIEERHMHKTCIAFDLMSVNVADLLVLP 960

BLAST of HG10023368 vs. TAIR 10
Match: AT3G16260.1 (tRNAse Z4 )

HSP 1 Score: 1019.6 bits (2635), Expect = 1.6e-297
Identity = 570/979 (58.22%), Postives = 671/979 (68.54%), Query Frame = 0

Query: 15  SSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKR---RRSTAAPSSLNFKRKNSSSLR 74
           SS  AL    F  +P I++  SP+     +  PP+    RRS  +P      R+  SS  
Sbjct: 20  SSSFALILRPFSLYPPIFASSSPA----PSRRPPRTAGYRRSGPSP-----PRRKWSSFE 79

Query: 75  ETEGKEKSVTMEDT------EGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPANTISY 134
           E + K +S   +D          SF FNKRRAEG DK D PKKNL+   R LNP NTI+Y
Sbjct: 80  EQKRKGRSPMEKDKAISFNHSSDSFEFNKRRAEGLDKVDKPKKNLKRNTRTLNPTNTIAY 139

Query: 135 VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSRVCSET 194
           VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSK+DHIFLSRVCSET
Sbjct: 140 VQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKVDHIFLSRVCSET 199

Query: 195 AGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPALS-SDV 254
           AGGLPGLLLTLAGIGE G+SVNVWGPSDLKYLVDAM+SFIP AAMVHTRSFGP+L+ SD 
Sbjct: 200 AGGLPGLLLTLAGIGEQGLSVNVWGPSDLKYLVDAMRSFIPRAAMVHTRSFGPSLNISDS 259

Query: 255 DAVHELSK-FCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDSEAR 314
                LSK   D  VLVDDEVVKISAIL+ P               R +E          
Sbjct: 260 APQIGLSKPKDDAYVLVDDEVVKISAILLEP--------------SRLEE---------- 319

Query: 315 SSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKAAAFGLKPGPKYRELQLGNSVMSDHQ 374
                  S  KPG+ +V+YVCELPEIKGKFDP KA A GL+ GPKY  LQ G SV SD +
Sbjct: 320 -------SGSKPGETAVIYVCELPEIKGKFDPKKAMALGLRAGPKYSYLQSGQSVKSDFK 379

Query: 375 KIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKVVTCV 434
            I VHPSDV+GPSVPGPVVLLVDCPTE+H  EL+S+ S++ YY    +  T+  K+V C+
Sbjct: 380 DITVHPSDVMGPSVPGPVVLLVDCPTESHAEELLSIPSMKTYYSCLDN-STDGAKLVNCI 439

Query: 435 IHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCPQLFP 494
           IHLSPAS+  S  Y+ W KRF  AQHI+AGH+ KN+  PILR+S++I ARLN+LCPQ FP
Sbjct: 440 IHLSPASVTNSSTYRSWMKRFHSAQHILAGHEAKNMEFPILRASSRITARLNYLCPQFFP 499

Query: 495 APGFWSHQQLTFPGSDSCASTEIEV---SNHHKSTLAENLLKFTLRPYAQLGLDRSSIPS 554
           APGFWSHQ      ++S   T +     SN  +S  AENLLKFTLRP+  LG+DRSSIPS
Sbjct: 500 APGFWSHQH----DNNSINPTSLSKCFDSNLGESISAENLLKFTLRPHGNLGVDRSSIPS 559

Query: 555 QESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDENKVPS 614
           + +    +  L S+IPEI      + QLW G          + N  M+EEPWL E+ VPS
Sbjct: 560 RLTALRVMDELLSEIPEISSKTEEIKQLWNG----------QHNKMMIEEPWLGESTVPS 619

Query: 615 CLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRYGVE 674
           CLENIRRDD+EIVLLGTGSSQPSKYRNV++IYI+LF +GS+LLDCGEGTLGQLKRRYG+E
Sbjct: 620 CLENIRRDDMEIVLLGTGSSQPSKYRNVTAIYIDLFSRGSILLDCGEGTLGQLKRRYGLE 679

Query: 675 GADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLDAYQ 734
           GAD AVR+LRCIWISHIHADHHTGL RILA RR+LL  + HEP +V+GPR LK +LDAYQ
Sbjct: 680 GADEAVRNLRCIWISHIHADHHTGLARILARRRELLKGLAHEPAIVVGPRSLKNFLDAYQ 739

Query: 735 RLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKTTSS 794
           RLEDLDM+FLDC++TT  S        + ++ S  E   SS N                S
Sbjct: 740 RLEDLDMEFLDCRNTTTTSW-------ASVETSRPEKNTSSGNAE-------------GS 799

Query: 795 LFVKGSRMQSYWKGPSSPV-DISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGVVL 854
           LF KGS MQS +K PSSP+ D S+A+P LK L +VL E GLE LISFPVVHCPQA+GV L
Sbjct: 800 LFSKGSLMQSIYKRPSSPLTDNSSALPFLKKLKKVLGEMGLEHLISFPVVHCPQAFGVSL 859

Query: 855 KAAGR--------------------------------------ATFEDSLVDEAMAKNHS 914
           KAA R                                      ATFED+LV+EA+AKNHS
Sbjct: 860 KAAERKNIAGDEIPGWKMVYSGDTRPCPEMVEASKGATVLIHEATFEDALVEEAVAKNHS 919

Query: 915 TTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLPKV 941
           TT EAI +G+SAG YR +LTHFSQRYPKIPV+ E HMH TCIAFD+MS+N+ADL VLPK+
Sbjct: 920 TTKEAIKVGSSAGVYRTVLTHFSQRYPKIPVIDESHMHNTCIAFDMMSINMADLHVLPKI 923

BLAST of HG10023368 vs. TAIR 10
Match: AT1G52160.1 (tRNAse Z3 )

HSP 1 Score: 987.6 bits (2552), Expect = 6.7e-288
Identity = 554/986 (56.19%), Postives = 647/986 (65.62%), Query Frame = 0

Query: 1   MPLPHLSSLRFLFLSSFPALSPSKFPFFPSIYSPKSPSLFTVIASSPPKRRRSTAAPSSL 60
           MP  H  +LR L L S  +   S FP     +SP+S SL T+ +SS              
Sbjct: 5   MPYLH-KNLRLLRLLSSKS---SPFPLSLRPFSPRSFSLSTLFSSS-------------- 64

Query: 61  NFKRKNSSSLRETEGKEKSVTMEDTEGTSFGFNKRRAEGKDKTDFPKKNLQLKVRKLNPA 120
                +SSS+   E    S     +   SF FNKRRAEG D TD  K+NL+ K +KLNP 
Sbjct: 65  ----SSSSSMENNEATNGS----KSSSNSFVFNKRRAEGFDITDKKKRNLERKSQKLNPT 124

Query: 121 NTISYVQILGTGMDTQDTSPSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHIFLSR 180
           NTI+Y QILGTGMDTQDTS SVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDH+FLSR
Sbjct: 125 NTIAYAQILGTGMDTQDTSSSVLLFFDKQRFIFNAGEGLQRFCTEHKIKLSKIDHVFLSR 184

Query: 181 VCSETAGGLPGLLLTLAGIGEVGMSVNVWGPSDLKYLVDAMKSFIPNAAMVHTRSFGPAL 240
           VCSETAGGLPGLLLTLAGIGE G+SVNVWGPSDL YLVDAMKSFIP AAMVHTRSFGP  
Sbjct: 185 VCSETAGGLPGLLLTLAGIGEEGLSVNVWGPSDLNYLVDAMKSFIPRAAMVHTRSFGP-- 244

Query: 241 SSDVDAVHELSKFCDPIVLVDDEVVKISAILVHPRPNRGFQPFDEDDMGRTQEERRNHDS 300
                     S   DPIVLV+DEVVKISAI++ P          E+D G           
Sbjct: 245 ----------SSTPDPIVLVNDEVVKISAIILKP-------CHSEEDSGN---------- 304

Query: 301 EARSSTAKTSSTVKPGDMSVVYVCELPEIKGKFDPSKA-AAFGLKPGPKYRELQLGNSVM 360
                        K GD+SVVYVCELPEI GKFD  KA   FG+KPGPKY  LQ G SV 
Sbjct: 305 -------------KSGDLSVVYVCELPEILGKFDLEKAKKVFGVKPGPKYSRLQSGESVK 364

Query: 361 SDHQKIMVHPSDVLGPSVPGPVVLLVDCPTEAHLLELMSLQSLRPYYEDFSSEQTETGKV 420
           SD + I VHPSDV+GPS+PGP+VLLVDCPTE+H  EL SL+SL  YY     EQT   K 
Sbjct: 365 SDERDITVHPSDVMGPSLPGPIVLLVDCPTESHAAELFSLKSLESYYSS-PDEQTIGAKF 424

Query: 421 VTCVIHLSPASILGSPNYQKWAKRFGLAQHIMAGHDRKNVAIPILRSSAKIAARLNHLCP 480
           V C+IHLSP+S+  SP YQ W K+F L QHI+AGH RKN+A PIL++S++IAARLN+LCP
Sbjct: 425 VNCIIHLSPSSVTSSPTYQSWMKKFHLTQHILAGHQRKNMAFPILKASSRIAARLNYLCP 484

Query: 481 QLFPAPGFWSHQQLTFPGSDSCASTEIEVSNHHKSTLAENLLKFTLRPYAQLGLDRSSIP 540
           Q FPAPGFW  Q       D   S +   SN  +S  AENLLKF LRP A  G+DRS IP
Sbjct: 485 QFFPAPGFWPSQLTDNSIIDPTPSNKCSSSNLAESISAENLLKFNLRPVAIRGIDRSCIP 544

Query: 541 SQESLPETISALHSDIPEILDAVHHVSQLWRGSAETDEKTPNEDNNAMVEEPWLDE--NK 600
           +  +  E +  L S+IPEI D    + Q W            + N  ++E+ WL E    
Sbjct: 545 APLTSSEVVDELLSEIPEIKDKSEEIKQFWN----------KQHNKTIIEKLWLSECNTV 604

Query: 601 VPSCLENIRRDDLEIVLLGTGSSQPSKYRNVSSIYINLFLKGSMLLDCGEGTLGQLKRRY 660
           +P+CLE IRRDD+EIV+LGTGSSQPSKYRNVS+I+I+LF +GS+LLDCGEGTLGQLKRRY
Sbjct: 605 LPNCLEKIRRDDMEIVILGTGSSQPSKYRNVSAIFIDLFSRGSLLLDCGEGTLGQLKRRY 664

Query: 661 GVEGADAAVRSLRCIWISHIHADHHTGLTRILALRRDLLWEIPHEPVLVIGPRQLKRYLD 720
           G++GAD AVR LRCIWISHIHADHHTGL RILALR  LL  + HEPV+V+GPR LKR+LD
Sbjct: 665 GLDGADEAVRKLRCIWISHIHADHHTGLARILALRSKLLKGVTHEPVIVVGPRPLKRFLD 724

Query: 721 AYQRLEDLDMQFLDCKHTTQASLEAFQNVASEIDKSPSESPISSTNGSSTPIDRATGQKT 780
           AYQRLEDLDM+FLDC+ TT  S  + +                            +G + 
Sbjct: 725 AYQRLEDLDMEFLDCRSTTATSWASLE----------------------------SGGEA 784

Query: 781 TSSLFVKGSRMQSYWKGPSSPVDISAAMPLLKCLNEVLNEAGLEALISFPVVHCPQAYGV 840
             SLF +GS MQS +K     +D S+ +  LK L +VL+E GL  LISFPVVHCPQAYGV
Sbjct: 785 EGSLFTQGSPMQSVFKRSDISMDNSSVLLCLKNLKKVLSEIGLNDLISFPVVHCPQAYGV 844

Query: 841 VLKAAGR--------------------------------------ATFEDSLVDEAMAKN 900
           V+KAA R                                      ATFED+L++EA+AKN
Sbjct: 845 VIKAAERVNSVGEQILGWKMVYSGDSRPCPETVEASRDATILIHEATFEDALIEEALAKN 883

Query: 901 HSTTSEAIDIGNSAGAYRIILTHFSQRYPKIPVVSERHMHKTCIAFDLMSVNVADLLVLP 946
           HSTT EAID+G++A  YRI+LTHFSQRYPKIPV+ E HMH TCIAFDLMS+N+ADL VLP
Sbjct: 905 HSTTKEAIDVGSAANVYRIVLTHFSQRYPKIPVIDESHMHNTCIAFDLMSINMADLHVLP 883