HG10023365 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023365 vs. NCBI nr
Match: XP_038899992.1 (uncharacterized protein LOC120087160 isoform X3 [Benincasa hispida])

HSP 1 Score: 782.7 bits (2020), Expect = 1.9e-222
Identity = 414/490 (84.49%), Postives = 435/490 (88.78%), Query Frame = 0

Query: 32  MCQGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRV 91
           MCQG YAYNGWSLNANMT KKEF+SAADQKKKRKTISQAW+PVCT  SPSEDLS+KDDRV
Sbjct: 1   MCQGLYAYNGWSLNANMTNKKEFRSAADQKKKRKTISQAWKPVCTQASPSEDLSVKDDRV 60

Query: 92  ESEDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVP 151
           E EDGS+VQEMDCRMHTST SA++VVEVAEEINVVTELSV SSAFPNM GD NLEGQSVP
Sbjct: 61  ELEDGSRVQEMDCRMHTSTASARNVVEVAEEINVVTELSVESSAFPNMDGDANLEGQSVP 120

Query: 152 SGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQ 211
           S EKFSVKLDVGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSKKEEFV         + 
Sbjct: 121 SDEKFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG---NSVD 180

Query: 212 FFATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEA 271
                   + S+  +AIKSP LDYSHFVSLPLA+HPELVEKL NFQN IL SSESCLDEA
Sbjct: 181 SVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLALHPELVEKLTNFQNSILRSSESCLDEA 240

Query: 272 EDSDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPS 331
           EDSDTNEDNTDNEVEVQHTV+APDVAVELKVDDKREQIKVNINIPVVSYP KTS+ASTPS
Sbjct: 241 EDSDTNEDNTDNEVEVQHTVEAPDVAVELKVDDKREQIKVNINIPVVSYPSKTSKASTPS 300

Query: 332 DLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGL 391
           DLGIDKSIFIKP TFHLTVLMLKLWNK+RVDAASEVLRGISSKIMDALDNR VLIRLKGL
Sbjct: 301 DLGIDKSIFIKPNTFHLTVLMLKLWNKDRVDAASEVLRGISSKIMDALDNRQVLIRLKGL 360

Query: 392 ECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMV 451
           +CMRGSLAKARVLYAPVEEI +EGRLL ACQVII+AFTEAGLVLEKDAKQKLK   +V  
Sbjct: 361 DCMRGSLAKARVLYAPVEEIGNEGRLLRACQVIINAFTEAGLVLEKDAKQKLKLHATV-- 420

Query: 452 DMAPQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASI 511
            M  +   SKKKKKFDSFDAREIFK YGSEEWGEYHIRE HLSQRF+FDENGYYH CASI
Sbjct: 421 -MNARHRKSKKKKKFDSFDAREIFKHYGSEEWGEYHIREVHLSQRFSFDENGYYHCCASI 480

Query: 512 PFPDEQMQVD 520
           PFPDE MQVD
Sbjct: 481 PFPDEHMQVD 484

BLAST of HG10023365 vs. NCBI nr
Match: XP_038899990.1 (uncharacterized protein LOC120087160 isoform X1 [Benincasa hispida])

HSP 1 Score: 775.0 bits (2000), Expect = 4.0e-220
Identity = 411/487 (84.39%), Postives = 432/487 (88.71%), Query Frame = 0

Query: 35  GQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESE 94
           G YAYNGWSLNANMT KKEF+SAADQKKKRKTISQAW+PVCT  SPSEDLS+KDDRVE E
Sbjct: 39  GLYAYNGWSLNANMTNKKEFRSAADQKKKRKTISQAWKPVCTQASPSEDLSVKDDRVELE 98

Query: 95  DGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGE 154
           DGS+VQEMDCRMHTST SA++VVEVAEEINVVTELSV SSAFPNM GD NLEGQSVPS E
Sbjct: 99  DGSRVQEMDCRMHTSTASARNVVEVAEEINVVTELSVESSAFPNMDGDANLEGQSVPSDE 158

Query: 155 KFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFFA 214
           KFSVKLDVGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSKKEEFV         +    
Sbjct: 159 KFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG---NSVDSVT 218

Query: 215 TGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDS 274
                + S+  +AIKSP LDYSHFVSLPLA+HPELVEKL NFQN IL SSESCLDEAEDS
Sbjct: 219 KASEKIQSIIDEAIKSPSLDYSHFVSLPLALHPELVEKLTNFQNSILRSSESCLDEAEDS 278

Query: 275 DTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDLG 334
           DTNEDNTDNEVEVQHTV+APDVAVELKVDDKREQIKVNINIPVVSYP KTS+ASTPSDLG
Sbjct: 279 DTNEDNTDNEVEVQHTVEAPDVAVELKVDDKREQIKVNINIPVVSYPSKTSKASTPSDLG 338

Query: 335 IDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECM 394
           IDKSIFIKP TFHLTVLMLKLWNK+RVDAASEVLRGISSKIMDALDNR VLIRLKGL+CM
Sbjct: 339 IDKSIFIKPNTFHLTVLMLKLWNKDRVDAASEVLRGISSKIMDALDNRQVLIRLKGLDCM 398

Query: 395 RGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMA 454
           RGSLAKARVLYAPVEEI +EGRLL ACQVII+AFTEAGLVLEKDAKQKLK   +V   M 
Sbjct: 399 RGSLAKARVLYAPVEEIGNEGRLLRACQVIINAFTEAGLVLEKDAKQKLKLHATV---MN 458

Query: 455 PQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFP 514
            +   SKKKKKFDSFDAREIFK YGSEEWGEYHIRE HLSQRF+FDENGYYH CASIPFP
Sbjct: 459 ARHRKSKKKKKFDSFDAREIFKHYGSEEWGEYHIREVHLSQRFSFDENGYYHCCASIPFP 518

Query: 515 DEQMQVD 520
           DE MQVD
Sbjct: 519 DEHMQVD 519

BLAST of HG10023365 vs. NCBI nr
Match: XP_038899991.1 (uncharacterized protein LOC120087160 isoform X2 [Benincasa hispida])

HSP 1 Score: 767.3 bits (1980), Expect = 8.4e-218
Identity = 409/487 (83.98%), Postives = 431/487 (88.50%), Query Frame = 0

Query: 35  GQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESE 94
           G YAYNGWSLNANMT KKEF+SAADQKKKRKTISQAW+PVCT  SPSEDLS+KDDRVE E
Sbjct: 39  GLYAYNGWSLNANMTNKKEFRSAADQKKKRKTISQAWKPVCTQASPSEDLSVKDDRVELE 98

Query: 95  DGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGE 154
           DGS+VQEMDCRMHTST SA++VVEVAEEINVVTELSV SSAFPNM GD NLEGQSVPS E
Sbjct: 99  DGSRVQEMDCRMHTSTASARNVVEVAEEINVVTELSVESSAFPNMDGDANLEGQSVPSDE 158

Query: 155 KFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFFA 214
           KFSVKLDVGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSKKEEFV         +    
Sbjct: 159 KFSVKLDVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKKEEFVVIEG---NSVDSVT 218

Query: 215 TGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDS 274
                + S+  +AIKSP LDYSHFVSLPLA+HPELVEKL NFQN IL SSESCLDEAEDS
Sbjct: 219 KASEKIQSIIDEAIKSPSLDYSHFVSLPLALHPELVEKLTNFQNSILRSSESCLDEAEDS 278

Query: 275 DTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDLG 334
           DTNEDNTDNEVEVQHTV+APDVAVELKVDDKREQIKVNINIPVVSYP KTS+ASTPSDLG
Sbjct: 279 DTNEDNTDNEVEVQHTVEAPDVAVELKVDDKREQIKVNINIPVVSYPSKTSKASTPSDLG 338

Query: 335 IDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECM 394
           IDKSIFIKP TFHLTVLMLKLWNK+RVDAASEVLRGISSKIMDALDNR VLIRLKGL+CM
Sbjct: 339 IDKSIFIKPNTFHLTVLMLKLWNKDRVDAASEVLRGISSKIMDALDNRQVLIRLKGLDCM 398

Query: 395 RGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMA 454
           RGSLAKARVLYAPVEEI +EGRLL AC +II+AFTEAGLVLEKDAKQKLK   +V   M 
Sbjct: 399 RGSLAKARVLYAPVEEIGNEGRLLRAC-LIINAFTEAGLVLEKDAKQKLKLHATV---MN 458

Query: 455 PQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFP 514
            +   SKKKKKFDSFDAREIFK YGSEEWGEYHIRE HLSQRF+FDENGYYH CASIPFP
Sbjct: 459 ARHRKSKKKKKFDSFDAREIFKHYGSEEWGEYHIREVHLSQRFSFDENGYYHCCASIPFP 518

Query: 515 DEQMQVD 520
           DE MQVD
Sbjct: 519 DEHMQVD 518

BLAST of HG10023365 vs. NCBI nr
Match: XP_022954063.1 (uncharacterized protein LOC111456441 isoform X1 [Cucurbita moschata])

HSP 1 Score: 765.4 bits (1975), Expect = 3.2e-217
Identity = 406/492 (82.52%), Postives = 429/492 (87.20%), Query Frame = 0

Query: 30  IPMCQGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDD 89
           +P   G Y YNGWSLNANM GKKEF+  ADQKKKRKTI+QAWRPVCT  SPSEDL +K+D
Sbjct: 21  VPQQPGLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQASPSEDLVVKND 80

Query: 90  RVESEDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQS 149
           RVESEDGS+VQE    +HTSTVSAQ+VVEVAEEINVVT+LSVGSSAFPN GGDTNLEGQS
Sbjct: 81  RVESEDGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPNEGGDTNLEGQS 140

Query: 150 VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEG 209
           VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFV         
Sbjct: 141 VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVVIEG---NS 200

Query: 210 MQFFATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLD 269
           +         + S+  +AIKSP LDYSHFVSLPLAIHPELVEKLINFQN ILGSSESCLD
Sbjct: 201 VDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILGSSESCLD 260

Query: 270 EAEDSDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRAST 329
           EAEDSDTNEDNTDNEVEV  TVK PDVAVELKVDDK E +KVNINIPVVSYPPK S+ ST
Sbjct: 261 EAEDSDTNEDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYPPKASKTST 320

Query: 330 PSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLK 389
           PSDLGIDKS+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+KIMDALDNRPVLI+LK
Sbjct: 321 PSDLGIDKSVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDNRPVLIKLK 380

Query: 390 GLECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSV 449
           GL+CMRGSLAKARVLYAPVEEI DEGRLL ACQVIIDAF EAGLVLEKDAKQKLK   +V
Sbjct: 381 GLDCMRGSLAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQKLKLHATV 440

Query: 450 MVDMAPQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCA 509
           M   A     +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRFAFDENGYYH CA
Sbjct: 441 M--NARHRKRNKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENGYYHCCA 500

Query: 510 SIPFPDEQMQVD 520
           SIPFPDEQMQV+
Sbjct: 501 SIPFPDEQMQVE 503

BLAST of HG10023365 vs. NCBI nr
Match: XP_022954065.1 (uncharacterized protein LOC111456441 isoform X2 [Cucurbita moschata])

HSP 1 Score: 764.6 bits (1973), Expect = 5.5e-217
Identity = 406/488 (83.20%), Postives = 428/488 (87.70%), Query Frame = 0

Query: 34  QGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVES 93
           QG Y YNGWSLNANM GKKEF+  ADQKKKRKTI+QAWRPVCT  SPSEDL +K+DRVES
Sbjct: 22  QGLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQASPSEDLVVKNDRVES 81

Query: 94  EDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSG 153
           EDGS+VQE    +HTSTVSAQ+VVEVAEEINVVT+LSVGSSAFPN GGDTNLEGQSVPSG
Sbjct: 82  EDGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPNEGGDTNLEGQSVPSG 141

Query: 154 EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFF 213
           EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFV         +   
Sbjct: 142 EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVVIEG---NSVDSV 201

Query: 214 ATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAED 273
                 + S+  +AIKSP LDYSHFVSLPLAIHPELVEKLINFQN ILGSSESCLDEAED
Sbjct: 202 TKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILGSSESCLDEAED 261

Query: 274 SDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDL 333
           SDTNEDNTDNEVEV  TVK PDVAVELKVDDK E +KVNINIPVVSYPPK S+ STPSDL
Sbjct: 262 SDTNEDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYPPKASKTSTPSDL 321

Query: 334 GIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLEC 393
           GIDKS+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+KIMDALDNRPVLI+LKGL+C
Sbjct: 322 GIDKSVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDNRPVLIKLKGLDC 381

Query: 394 MRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDM 453
           MRGSLAKARVLYAPVEEI DEGRLL ACQVIIDAF EAGLVLEKDAKQKLK   +VM   
Sbjct: 382 MRGSLAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQKLKLHATVM--N 441

Query: 454 APQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPF 513
           A     +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRFAFDENGYYH CASIPF
Sbjct: 442 ARHRKRNKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENGYYHCCASIPF 500

Query: 514 PDEQMQVD 520
           PDEQMQV+
Sbjct: 502 PDEQMQVE 500

BLAST of HG10023365 vs. ExPASy Swiss-Prot
Match: Q9D8Z1 (Activating signal cointegrator 1 complex subunit 1 OS=Mus musculus OX=10090 GN=Ascc1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.3e-15
Identity = 57/195 (29.23%), Postives = 92/195 (47.18%), Query Frame = 0

Query: 330 DLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK-IMDALDNRPVLIRLKG 389
           D G+D +IF  PK  HLT+ ML L +++ +    E+L+    + I D    RP+ + + G
Sbjct: 163 DRGVDSTIFQNPKKLHLTIGMLVLLSEQEIQQTCEILQRCKEEFINDISGGRPLEVEMAG 222

Query: 390 LECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVM 449
           +E M    A   VLYA V       RL      +++ F   GL++++    KL    +VM
Sbjct: 223 IEYMNDDPAMVDVLYAKVHMKDGSNRLQELVDRVLERFQSLGLIVKEWTSVKL--HATVM 282

Query: 450 VDMAPQCLNSKKK------------KKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFA 509
             +  +  N++ +            K+ +SFD R I K + +  +G   +   H+SQRF 
Sbjct: 283 NTLLRKDPNAEGRYNLYTADGKYIFKERESFDGRNILKTFENFYFGSLRLNSIHISQRFT 342

Query: 510 FDENGYYHSCASIPF 512
            D  G Y SC  + F
Sbjct: 343 VDSFGNYASCGHVDF 355

BLAST of HG10023365 vs. ExPASy Swiss-Prot
Match: Q8N9N2 (Activating signal cointegrator 1 complex subunit 1 OS=Homo sapiens OX=9606 GN=ASCC1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.9e-08
Identity = 45/165 (27.27%), Postives = 76/165 (46.06%), Query Frame = 0

Query: 325 ASTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSK-IMDALDNRPVL 384
           A    D G+D SIF  PK  HLT+ ML L ++E +    E+L+    + I D    +P+ 
Sbjct: 187 AKCSMDHGVDSSIFQNPKKLHLTIGMLVLLSEEEIQQTCEMLQQCKEEFINDISGGKPLE 246

Query: 385 IRLKGLECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKF 444
           + + G+E M        VLYA V       RL      +++ F  +GL++++     +K 
Sbjct: 247 VEMAGIEYMNDDPGMVDVLYAKVHMKDGSNRLQELVDRVLERFQASGLIVKE--WNSVKL 306

Query: 445 DFSVMVDMAPQCLNSKKK------------KKFDSFDAREIFKQY 477
             +VM  +  +  N++ +            K+ +SFD R I K +
Sbjct: 307 HATVMNTLFRKDPNAEGRYNLYTAEGKYIFKERESFDGRNILKSF 349

BLAST of HG10023365 vs. ExPASy TrEMBL
Match: A0A6J1GQ24 (uncharacterized protein LOC111456441 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456441 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.5e-217
Identity = 406/492 (82.52%), Postives = 429/492 (87.20%), Query Frame = 0

Query: 30  IPMCQGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDD 89
           +P   G Y YNGWSLNANM GKKEF+  ADQKKKRKTI+QAWRPVCT  SPSEDL +K+D
Sbjct: 21  VPQQPGLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQASPSEDLVVKND 80

Query: 90  RVESEDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQS 149
           RVESEDGS+VQE    +HTSTVSAQ+VVEVAEEINVVT+LSVGSSAFPN GGDTNLEGQS
Sbjct: 81  RVESEDGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPNEGGDTNLEGQS 140

Query: 150 VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEG 209
           VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFV         
Sbjct: 141 VPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVVIEG---NS 200

Query: 210 MQFFATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLD 269
           +         + S+  +AIKSP LDYSHFVSLPLAIHPELVEKLINFQN ILGSSESCLD
Sbjct: 201 VDSVTKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILGSSESCLD 260

Query: 270 EAEDSDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRAST 329
           EAEDSDTNEDNTDNEVEV  TVK PDVAVELKVDDK E +KVNINIPVVSYPPK S+ ST
Sbjct: 261 EAEDSDTNEDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYPPKASKTST 320

Query: 330 PSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLK 389
           PSDLGIDKS+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+KIMDALDNRPVLI+LK
Sbjct: 321 PSDLGIDKSVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDNRPVLIKLK 380

Query: 390 GLECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSV 449
           GL+CMRGSLAKARVLYAPVEEI DEGRLL ACQVIIDAF EAGLVLEKDAKQKLK   +V
Sbjct: 381 GLDCMRGSLAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQKLKLHATV 440

Query: 450 MVDMAPQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCA 509
           M   A     +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRFAFDENGYYH CA
Sbjct: 441 M--NARHRKRNKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENGYYHCCA 500

Query: 510 SIPFPDEQMQVD 520
           SIPFPDEQMQV+
Sbjct: 501 SIPFPDEQMQVE 503

BLAST of HG10023365 vs. ExPASy TrEMBL
Match: A0A6J1GRE7 (uncharacterized protein LOC111456441 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456441 PE=4 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.6e-217
Identity = 406/488 (83.20%), Postives = 428/488 (87.70%), Query Frame = 0

Query: 34  QGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVES 93
           QG Y YNGWSLNANM GKKEF+  ADQKKKRKTI+QAWRPVCT  SPSEDL +K+DRVES
Sbjct: 22  QGLYTYNGWSLNANMAGKKEFRRVADQKKKRKTITQAWRPVCTQASPSEDLVVKNDRVES 81

Query: 94  EDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSG 153
           EDGS+VQE    +HTSTVSAQ+VVEVAEEINVVT+LSVGSSAFPN GGDTNLEGQSVPSG
Sbjct: 82  EDGSRVQE----IHTSTVSAQNVVEVAEEINVVTDLSVGSSAFPNEGGDTNLEGQSVPSG 141

Query: 154 EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFF 213
           EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFV         +   
Sbjct: 142 EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVVIEG---NSVDSV 201

Query: 214 ATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAED 273
                 + S+  +AIKSP LDYSHFVSLPLAIHPELVEKLINFQN ILGSSESCLDEAED
Sbjct: 202 TKASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILGSSESCLDEAED 261

Query: 274 SDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDL 333
           SDTNEDNTDNEVEV  TVK PDVAVELKVDDK E +KVNINIPVVSYPPK S+ STPSDL
Sbjct: 262 SDTNEDNTDNEVEVHDTVKEPDVAVELKVDDKEEHVKVNINIPVVSYPPKASKTSTPSDL 321

Query: 334 GIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLEC 393
           GIDKS+FIKPKTFHLTVLMLKLWNKERVDAASEVLRGIS+KIMDALDNRPVLI+LKGL+C
Sbjct: 322 GIDKSVFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISTKIMDALDNRPVLIKLKGLDC 381

Query: 394 MRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDM 453
           MRGSLAKARVLYAPVEEI DEGRLL ACQVIIDAF EAGLVLEKDAKQKLK   +VM   
Sbjct: 382 MRGSLAKARVLYAPVEEIDDEGRLLRACQVIIDAFDEAGLVLEKDAKQKLKLHATVM--N 441

Query: 454 APQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPF 513
           A     +K+KKKFDSFD REIFKQYGSEEWG YHIREAHLSQRFAFDENGYYH CASIPF
Sbjct: 442 ARHRKRNKRKKKFDSFDTREIFKQYGSEEWGVYHIREAHLSQRFAFDENGYYHCCASIPF 500

Query: 514 PDEQMQVD 520
           PDEQMQV+
Sbjct: 502 PDEQMQVE 500

BLAST of HG10023365 vs. ExPASy TrEMBL
Match: A0A5D3C0W5 (Activating signal cointegrator 1 complex subunit 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002070 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 8.5e-216
Identity = 427/560 (76.25%), Postives = 448/560 (80.00%), Query Frame = 0

Query: 1   MSGRGSGANGNNAGSGIAS---------------------------TTHVVLELTSIPMC 60
           +SGRGSGANGNN    + +                           TT  VL  T     
Sbjct: 7   VSGRGSGANGNNGKRQLCARENGESMRQCCKWRPNSLLGVDRFLKYTTPYVLHQT----- 66

Query: 61  QGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVES 120
            G YAYNGW LNANMTGK EF+SAADQKKKRKTISQAWRPVCTH   SEDLS+KDDRVES
Sbjct: 67  -GLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHACSSEDLSVKDDRVES 126

Query: 121 EDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSG 180
           EDGSQVQEMDCRMH+ST SAQ  VEV EEINVVTELSV      N+GGDTNLEGQSVPSG
Sbjct: 127 EDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSV------NVGGDTNLEGQSVPSG 186

Query: 181 EKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFF 240
           EKFSVKL+VGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSK+EEFV         +   
Sbjct: 187 EKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEFVVIEG---NSVDSV 246

Query: 241 ATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAED 300
                 + S+  +A KSP LDYSHFVSLPLAIHPELVEKLINFQN IL SSESCLDE ED
Sbjct: 247 TKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESCLDETED 306

Query: 301 SDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDL 360
           SDTNEDNTDNEVEVQHTV APDVAVEL+VDDKREQIK NINIPVVSY PKTS+ STPSDL
Sbjct: 307 SDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVSYLPKTSKVSTPSDL 366

Query: 361 GIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLEC 420
           GIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGL+C
Sbjct: 367 GIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLDC 426

Query: 421 MRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKF-------- 480
           MRGSLAKARVLYAPVEEI DEGRLL ACQV+IDAFTEAGLVLEKDAK KLKF        
Sbjct: 427 MRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDAKHKLKFLLFANLYV 486

Query: 481 -DFSVMVDMAP--QCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDEN 520
            +  +   MAP  Q L+SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRF FDEN
Sbjct: 487 YNPELFDFMAPQSQSLSSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVFDEN 546

BLAST of HG10023365 vs. ExPASy TrEMBL
Match: A0A1S3CGC2 (activating signal cointegrator 1 complex subunit 1 OS=Cucumis melo OX=3656 GN=LOC103500600 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.7e-214
Identity = 412/504 (81.75%), Postives = 429/504 (85.12%), Query Frame = 0

Query: 20  TTHVVLELTSIPMCQGQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGS 79
           TT  VL  T      G YAYNGW LNANMTGK EF+SAADQKKKRKTISQAWRPVCTH  
Sbjct: 29  TTPYVLHQT------GLYAYNGWRLNANMTGKTEFKSAADQKKKRKTISQAWRPVCTHAC 88

Query: 80  PSEDLSLKDDRVESEDGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNM 139
            SEDLS+KDDRVESEDGSQVQEMDCRMH+ST SAQ  VEV EEINVVTELSV      N+
Sbjct: 89  SSEDLSVKDDRVESEDGSQVQEMDCRMHSSTASAQ-PVEVVEEINVVTELSV------NV 148

Query: 140 GGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEF 199
           GGDTNLEGQSVPSGEKFSVKL+VGSSLIRFVRGKGGSTQE+IEEEMGVKIMIPSSK+EEF
Sbjct: 149 GGDTNLEGQSVPSGEKFSVKLNVGSSLIRFVRGKGGSTQERIEEEMGVKIMIPSSKREEF 208

Query: 200 VGDSSYLPEGMQFFATGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNI 259
           V         +         + S+  +A KSP LDYSHFVSLPLAIHPELVEKLINFQN 
Sbjct: 209 VVIEG---NSVDSVTKASEKIQSIIDEATKSPSLDYSHFVSLPLAIHPELVEKLINFQNS 268

Query: 260 ILGSSESCLDEAEDSDTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVS 319
           IL SSESCLDE EDSDTNEDNTDNEVEVQHTV APDVAVEL+VDDKREQIK NINIPVVS
Sbjct: 269 ILRSSESCLDETEDSDTNEDNTDNEVEVQHTVNAPDVAVELQVDDKREQIKANINIPVVS 328

Query: 320 YPPKTSRASTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDAL 379
           Y PKTS+ STPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDAL
Sbjct: 329 YLPKTSKVSTPSDLGIDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDAL 388

Query: 380 DNRPVLIRLKGLECMRGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDA 439
           DNRPVLIRLKGL+CMRGSLAKARVLYAPVEEI DEGRLL ACQV+IDAFTEAGLVLEKDA
Sbjct: 389 DNRPVLIRLKGLDCMRGSLAKARVLYAPVEEIGDEGRLLRACQVVIDAFTEAGLVLEKDA 448

Query: 440 KQKLKFDFSVMVDMAPQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAF 499
           K KLK   +V   M  +   SKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRF F
Sbjct: 449 KHKLKLHATV---MNARHRKSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFVF 508

Query: 500 DENGYYHSCASIPFPDEQ--MQVD 520
           DENGYYH CASIPFP EQ  MQVD
Sbjct: 509 DENGYYHCCASIPFPHEQQHMQVD 513

BLAST of HG10023365 vs. ExPASy TrEMBL
Match: A0A0A0K963 (KH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447090 PE=4 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 7.4e-212
Identity = 406/488 (83.20%), Postives = 428/488 (87.70%), Query Frame = 0

Query: 35  GQYAYNGWSLNANMTGKKEFQSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESE 94
           G YAYNGW LNANMTGK EF+SAADQKKKRKTISQAWRPVCTH  PSEDLS++DDRVESE
Sbjct: 84  GLYAYNGWRLNANMTGKTEFRSAADQKKKRKTISQAWRPVCTHACPSEDLSVEDDRVESE 143

Query: 95  DGSQVQEMDCRMHTSTVSAQHVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGE 154
           DGSQVQEMD RMHTST SAQ  VEVAEEINVVTELSV      NMGGDTNLEGQSV SGE
Sbjct: 144 DGSQVQEMDVRMHTST-SAQ-PVEVAEEINVVTELSV------NMGGDTNLEGQSVTSGE 203

Query: 155 KFSVKLDVGSSLIRFVRGKGGSTQEKIEEEMGVKIMIPSSKKEEFVGDSSYLPEGMQFFA 214
           KFSVKLDVGSSLIRFVRGKGGSTQE+IE+EMGVKIMIPSSK+EEFV         +    
Sbjct: 204 KFSVKLDVGSSLIRFVRGKGGSTQERIEKEMGVKIMIPSSKREEFVVIEG---NSVDSVT 263

Query: 215 TGGFGVGSM--KAIKSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDS 274
                + S+  +AIKSP LDYSHFVSLPLAIHPELVEKLINFQN IL SSESCLD+AEDS
Sbjct: 264 KASEKIQSIIDEAIKSPSLDYSHFVSLPLAIHPELVEKLINFQNSILRSSESCLDDAEDS 323

Query: 275 DTNEDNTDNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDLG 334
           DTNED+TDNEVEVQHTV APDVAVEL+VD+KREQIKVNINIP+VSY PKTS+ STPSDLG
Sbjct: 324 DTNEDHTDNEVEVQHTVNAPDVAVELQVDNKREQIKVNINIPIVSYLPKTSKVSTPSDLG 383

Query: 335 IDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECM 394
           IDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMD LDNRPVLIRLKGL+CM
Sbjct: 384 IDKSIFIKPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDTLDNRPVLIRLKGLDCM 443

Query: 395 RGSLAKARVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMA 454
           RGSLAKARVLYAPVEEI DEGRLL ACQ+II+AFTEAGLVLEKDAKQKLK   +V   M 
Sbjct: 444 RGSLAKARVLYAPVEEIGDEGRLLRACQLIINAFTEAGLVLEKDAKQKLKLHATV---MN 503

Query: 455 PQCLNSKKKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFP 514
            +   SKKKKKFDSFDAREIFK+YGSEEWGEYHIREAHLSQRFAFDENGYYH CASIPFP
Sbjct: 504 ARHRKSKKKKKFDSFDAREIFKEYGSEEWGEYHIREAHLSQRFAFDENGYYHCCASIPFP 557

Query: 515 DEQ-MQVD 520
            EQ MQVD
Sbjct: 564 HEQHMQVD 557

BLAST of HG10023365 vs. TAIR 10
Match: AT3G16230.2 (Predicted eukaryotic LigT )

HSP 1 Score: 399.8 bits (1026), Expect = 3.3e-111
Identity = 238/467 (50.96%), Postives = 302/467 (64.67%), Query Frame = 0

Query: 55  QSAADQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESEDGSQVQEMDCRMHTSTVSAQ 114
           +SA D  KK+K ++  WRP+ T  S           V +E G++VQE             
Sbjct: 38  KSAMDGYKKQKMVNLVWRPISTQTS----------SVIAEAGNEVQE------------- 97

Query: 115 HVVEVAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKG 174
            V + ++  +V  E+  G +A             SV S  K SV L+VG+SLI+F+RGK 
Sbjct: 98  -VAQCSKSSDVSKEVIEGETA-------------SVVSAGKHSVSLEVGASLIKFIRGKE 157

Query: 175 GSTQEKIEEEMGVKIMIPSSKKEEFV----GDSSYLPEGMQFFATGGFGVGSMKAIKSPR 234
           G+TQ K+EEEMGVKI++PSS+ ++ +    G    + +  +  AT        + ++SP 
Sbjct: 158 GTTQMKLEEEMGVKIILPSSRNKDHISIEGGSVDCVTKASKRIAT-----IIDEVVRSPS 217

Query: 235 LDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDSDTNEDNTDNEVEVQHTVK 294
           LDYSHFVSLPLAIHPELV+KL+NFQN ILG   S   + +D   N   T           
Sbjct: 218 LDYSHFVSLPLAIHPELVDKLVNFQNSILG-IHSIASDKQDDQANRATT----------- 277

Query: 295 APDVAVELKVDDKREQIKVNI-NIPVVSYPPKT-SRASTPSDLGIDKSIFIKPKTFHLTV 354
              VAV+LK + +  Q+ V I +IP+VSYPPK  S++ST  DLGI+KSIFIKP TFHLTV
Sbjct: 278 --SVAVDLKANSETNQVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTV 337

Query: 355 LMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECMRGSLAKARVLYAPVEE 414
           +MLKLWNK+RV+AA +VL+ I   +MDALDN+PV IRLKGL+CMRG L K RVLYAPVEE
Sbjct: 338 VMLKLWNKDRVNAACDVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEE 397

Query: 415 ISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMAPQCLNSKKKKKFDSFD 474
           I DEGRLL ACQVI DAF +AGLVLEKDAKQ LK   +VM +   +      KKK ++FD
Sbjct: 398 IGDEGRLLRACQVITDAFVKAGLVLEKDAKQSLKLHVTVM-NARHRKRRKNNKKKMETFD 447

Query: 475 AREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFPDEQ 516
           AREI KQ+G+E+WGEY I+EAHLSQRF FD+NGYY  C SIPFP EQ
Sbjct: 458 AREIHKQFGNEDWGEYLIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 447

BLAST of HG10023365 vs. TAIR 10
Match: AT3G16230.1 (Predicted eukaryotic LigT )

HSP 1 Score: 396.7 bits (1018), Expect = 2.8e-110
Identity = 236/463 (50.97%), Postives = 299/463 (64.58%), Query Frame = 0

Query: 59  DQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESEDGSQVQEMDCRMHTSTVSAQHVVE 118
           D  KK+K ++  WRP+ T  S           V +E G++VQE              V +
Sbjct: 2   DGYKKQKMVNLVWRPISTQTS----------SVIAEAGNEVQE--------------VAQ 61

Query: 119 VAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQ 178
            ++  +V  E+  G +A             SV S  K SV L+VG+SLI+F+RGK G+TQ
Sbjct: 62  CSKSSDVSKEVIEGETA-------------SVVSAGKHSVSLEVGASLIKFIRGKEGTTQ 121

Query: 179 EKIEEEMGVKIMIPSSKKEEFV----GDSSYLPEGMQFFATGGFGVGSMKAIKSPRLDYS 238
            K+EEEMGVKI++PSS+ ++ +    G    + +  +  AT        + ++SP LDYS
Sbjct: 122 MKLEEEMGVKIILPSSRNKDHISIEGGSVDCVTKASKRIAT-----IIDEVVRSPSLDYS 181

Query: 239 HFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDSDTNEDNTDNEVEVQHTVKAPDV 298
           HFVSLPLAIHPELV+KL+NFQN ILG   S   + +D   N   T              V
Sbjct: 182 HFVSLPLAIHPELVDKLVNFQNSILG-IHSIASDKQDDQANRATT-------------SV 241

Query: 299 AVELKVDDKREQIKVNI-NIPVVSYPPKT-SRASTPSDLGIDKSIFIKPKTFHLTVLMLK 358
           AV+LK + +  Q+ V I +IP+VSYPPK  S++ST  DLGI+KSIFIKP TFHLTV+MLK
Sbjct: 242 AVDLKANSETNQVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTVVMLK 301

Query: 359 LWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECMRGSLAKARVLYAPVEEISDE 418
           LWNK+RV+AA +VL+ I   +MDALDN+PV IRLKGL+CMRG L K RVLYAPVEEI DE
Sbjct: 302 LWNKDRVNAACDVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEEIGDE 361

Query: 419 GRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMAPQCLNSKKKKKFDSFDAREI 478
           GRLL ACQVI DAF +AGLVLEKDAKQ LK   +VM +   +      KKK ++FDAREI
Sbjct: 362 GRLLRACQVITDAFVKAGLVLEKDAKQSLKLHVTVM-NARHRKRRKNNKKKMETFDAREI 407

Query: 479 FKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFPDEQ 516
            KQ+G+E+WGEY I+EAHLSQRF FD+NGYY  C SIPFP EQ
Sbjct: 422 HKQFGNEDWGEYLIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 407

BLAST of HG10023365 vs. TAIR 10
Match: AT3G16230.3 (Predicted eukaryotic LigT )

HSP 1 Score: 396.7 bits (1018), Expect = 2.8e-110
Identity = 236/463 (50.97%), Postives = 299/463 (64.58%), Query Frame = 0

Query: 59  DQKKKRKTISQAWRPVCTHGSPSEDLSLKDDRVESEDGSQVQEMDCRMHTSTVSAQHVVE 118
           D  KK+K ++  WRP+ T  S           V +E G++VQE              V +
Sbjct: 2   DGYKKQKMVNLVWRPISTQTS----------SVIAEAGNEVQE--------------VAQ 61

Query: 119 VAEEINVVTELSVGSSAFPNMGGDTNLEGQSVPSGEKFSVKLDVGSSLIRFVRGKGGSTQ 178
            ++  +V  E+  G +A             SV S  K SV L+VG+SLI+F+RGK G+TQ
Sbjct: 62  CSKSSDVSKEVIEGETA-------------SVVSAGKHSVSLEVGASLIKFIRGKEGTTQ 121

Query: 179 EKIEEEMGVKIMIPSSKKEEFV----GDSSYLPEGMQFFATGGFGVGSMKAIKSPRLDYS 238
            K+EEEMGVKI++PSS+ ++ +    G    + +  +  AT        + ++SP LDYS
Sbjct: 122 MKLEEEMGVKIILPSSRNKDHISIEGGSVDCVTKASKRIAT-----IIDEVVRSPSLDYS 181

Query: 239 HFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDSDTNEDNTDNEVEVQHTVKAPDV 298
           HFVSLPLAIHPELV+KL+NFQN ILG   S   + +D   N   T              V
Sbjct: 182 HFVSLPLAIHPELVDKLVNFQNSILG-IHSIASDKQDDQANRATT-------------SV 241

Query: 299 AVELKVDDKREQIKVNI-NIPVVSYPPKT-SRASTPSDLGIDKSIFIKPKTFHLTVLMLK 358
           AV+LK + +  Q+ V I +IP+VSYPPK  S++ST  DLGI+KSIFIKP TFHLTV+MLK
Sbjct: 242 AVDLKANSETNQVNVGIKSIPIVSYPPKAKSKSSTLLDLGIEKSIFIKPSTFHLTVVMLK 301

Query: 359 LWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECMRGSLAKARVLYAPVEEISDE 418
           LWNK+RV+AA +VL+ I   +MDALDN+PV IRLKGL+CMRG L K RVLYAPVEEI DE
Sbjct: 302 LWNKDRVNAACDVLKSIFPSVMDALDNKPVFIRLKGLDCMRGPLDKTRVLYAPVEEIGDE 361

Query: 419 GRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMAPQCLNSKKKKKFDSFDAREI 478
           GRLL ACQVI DAF +AGLVLEKDAKQ LK   +VM +   +      KKK ++FDAREI
Sbjct: 362 GRLLRACQVITDAFVKAGLVLEKDAKQSLKLHVTVM-NARHRKRRKNNKKKMETFDAREI 407

Query: 479 FKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFPDEQ 516
            KQ+G+E+WGEY I+EAHLSQRF FD+NGYY  C SIPFP EQ
Sbjct: 422 HKQFGNEDWGEYLIQEAHLSQRFVFDQNGYYRCCGSIPFPGEQ 407

BLAST of HG10023365 vs. TAIR 10
Match: AT3G16220.1 (Predicted eukaryotic LigT )

HSP 1 Score: 222.6 bits (566), Expect = 7.2e-58
Identity = 129/293 (44.03%), Postives = 170/293 (58.02%), Query Frame = 0

Query: 221 GSMKAI-KSPRLDYSHFVSLPLAIHPELVEKLINFQNIILGSSESCLDEAEDSDTNEDNT 280
           G+ K I +  R  ++HFVSLPLAI+P+L + +  FQN +LG+++                
Sbjct: 23  GNQKQIGQGQREVFTHFVSLPLAIYPDLTKNIEAFQNSVLGNND---------------- 82

Query: 281 DNEVEVQHTVKAPDVAVELKVDDKREQIKVNINIPVVSYPPKTSRASTPSDLGIDKSIFI 340
                                   ++ +K                 ST +++GI+KSIF+
Sbjct: 83  ------------------------KDPLKFQ---------------STLAEMGIEKSIFV 142

Query: 341 KPKTFHLTVLMLKLWNKERVDAASEVLRGISSKIMDALDNRPVLIRLKGLECMRGSLAKA 400
            PKTFHLTV+MLKL N E V  A  +L+ I S +  AL NRPV IRL+GLECM GSL K 
Sbjct: 143 TPKTFHLTVVMLKLENNESVVKAQNILQSIFSNVRQALKNRPVFIRLRGLECMSGSLDKT 202

Query: 401 RVLYAPVEEISDEGRLLHACQVIIDAFTEAGLVLEKDAKQKLKFDFSVMVDMAPQCLNSK 460
           RVLYAPVEE+  EGRLL+AC VIIDAF   G    KDAK +LK   ++M           
Sbjct: 203 RVLYAPVEEVGREGRLLNACHVIIDAFENVGFA-GKDAKSRLKLHATLM----NASYRKD 255

Query: 461 KKKKFDSFDAREIFKQYGSEEWGEYHIREAHLSQRFAFDENGYYHSCASIPFP 513
           K KK D+FDAREI K++ +++WG Y IREAH+SQR+ +D NGY+H CAS+PFP
Sbjct: 263 KSKKMDTFDAREIHKEFENKDWGTYLIREAHISQRYKYDPNGYFHCCASLPFP 255