HG10023340 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023340 vs. NCBI nr
Match: XP_038899337.1 (protein ROLLING AND ERECT LEAF 2-like [Benincasa hispida])

HSP 1 Score: 1305.8 bits (3378), Expect = 0.0e+00
Identity = 687/714 (96.22%), Postives = 692/714 (96.92%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG+A AQSAAA AYNSLPPPPPPLPGSPGMPLQQ  SMFEIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAAAQSAAAVAYNSLPPPPPPLPGSPGMPLQQGKSMFEIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VIEEVDENDFEIECSVGPLRRR+ NRDGGGR GRTGPGELAEEENGPPPPL       PP
Sbjct: 121 VIEEVDENDFEIECSVGPLRRRRSNRDGGGRGGRTGPGELAEEENGPPPPL-------PP 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           SSENRRVPAPS QDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS
Sbjct: 181 SSENRRVPAPSAQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFD EENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDREENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVI TIMQSQEEEMKLKVKCQETEKELAR
Sbjct: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIHTIMQSQEEEMKLKVKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDK-DAIAERQAAVEAMEKRLEEEREEHQKLC 660
           KSKQFKDWQKKY+QRRASNADE NLEE  DK DAIAERQAAVEA+EKRLEEEREEHQKLC
Sbjct: 601 KSKQFKDWQKKYMQRRASNADEVNLEENGDKDDAIAERQAAVEAVEKRLEEEREEHQKLC 660

Query: 661 LHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQ 714
           LHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPL NRP SQT+AQ
Sbjct: 661 LHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLLNRPLSQTTAQ 707

BLAST of HG10023340 vs. NCBI nr
Match: XP_008462152.1 (PREDICTED: uncharacterized protein LOC103500575 [Cucumis melo] >XP_016902857.1 PREDICTED: uncharacterized protein LOC103500575 [Cucumis melo])

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 659/717 (91.91%), Postives = 676/717 (94.28%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG+AV QSA AA YNSLPPPPPPLPGSPGM L       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GRTGPGELAEEENGPP P        P 
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPF-------PA 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           S E+RRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEEPPSS
Sbjct: 181 SGESRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLVQL+NGMASMWE MHFHHGSQLKVVAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQSLLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQSLLHAWQDHLEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE  DKDAIAERQAAVEA+EKRLEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN  Q+QT+ QGVGT
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQGVGT 703

BLAST of HG10023340 vs. NCBI nr
Match: XP_004141776.1 (nitrate regulatory gene2 protein [Cucumis sativus] >XP_011659565.1 nitrate regulatory gene2 protein [Cucumis sativus] >XP_031744907.1 nitrate regulatory gene2 protein [Cucumis sativus] >KGN45358.1 hypothetical protein Csa_015836 [Cucumis sativus])

HSP 1 Score: 1260.4 bits (3260), Expect = 0.0e+00
Identity = 655/714 (91.74%), Postives = 675/714 (94.54%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG++V QSAAAA YNSLPPPPPPLPGSPGMPL       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSSVVQSAAAAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GR GPGELAEEENGPPPP        PP
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPF-------PP 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           SSENRRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEEPPSS
Sbjct: 181 SSENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAATEKSGEEPPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKG+INLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGSINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLV L+NGMASMWE MHFHHGSQLK VAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVHLINGMASMWETMHFHHGSQLKAVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQ LLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE +DKDAIAERQAAVEA+EK+LEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQG 715
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN PQ+QT+ QG
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTQG 700

BLAST of HG10023340 vs. NCBI nr
Match: XP_022954122.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata] >XP_022954123.1 nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1259.6 bits (3258), Expect = 0.0e+00
Identity = 667/720 (92.64%), Postives = 683/720 (94.86%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMK+AVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGAAVAQSAAAAA---YNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKR 120
           PPA SSLPG AVAQSAA AA   YNSLPPPPPPLPGSPGMPL+  TSMFEIKASKVEPKR
Sbjct: 61  PPAASSLPGVAVAQSAAVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVEPKR 120

Query: 121 VEPVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNR 180
           VE VIEEVDENDFEIECSVGPLRRR  NR+GGGR GRTG GELAEEENGPPPPLPPS NR
Sbjct: 121 VETVIEEVDENDFEIECSVGPLRRR-SNREGGGRGGRTGLGELAEEENGPPPPLPPSLNR 180

Query: 181 PPPSSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEP 240
           PPP +ENRR  +PSPQD+TYDYLFSVENMPAPTLS VEDF TNTEAIERRAA EKSG E 
Sbjct: 181 PPPPNENRRAHSPSPQDATYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAVEKSGGEL 240

Query: 241 PSSSAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVGFP S EGKR VKGN +LLQIFMELDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHG QLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGGQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKE 600
           RVRSPPIQSLLHVWQDHLEKLPDEVLRN+IFTFATVI TI+QSQEEEMKLKVKCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKVKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRRA NADE+N EET DKDAIAERQAAVEA+EKRLEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNADEANQEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQT+A+ VGT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTTAR-VGT 718

BLAST of HG10023340 vs. NCBI nr
Match: KAA0059290.1 (uncharacterized protein E6C27_scaffold242G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 658/717 (91.77%), Postives = 675/717 (94.14%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG+AV QSA AA YNSLPPPPPPLPGSPGM L       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GRTGPGELAEEENGPP P        P 
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPF-------PA 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           S E+RRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEE PSS
Sbjct: 181 SGESRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEELPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLVQL+NGMASMWE MHFHHGSQLKVVAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQSLLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQSLLHAWQDHLEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE  DKDAIAERQAAVEA+EKRLEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN  Q+QT+ QGVGT
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQGVGT 703

BLAST of HG10023340 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 6.1e-60
Identity = 208/732 (28.42%), Postives = 328/732 (44.81%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC QS+I+++E+VSRCK RK ++K  V AR   + +H+ Y  SL+  G+ L  ++  E 
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  ------GPPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGM-PLQQATSMFEIKASK 120
                  PP+PS                   PPPPPP P  P + P  + T+      S 
Sbjct: 61  PLHLHHNPPSPSP------------------PPPPPPRPPPPPLSPGSETTTWTTTTTSS 120

Query: 121 VEPKRVEPVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLP 180
           V P                                                   PPPP P
Sbjct: 121 VLP---------------------------------------------------PPPPPP 180

Query: 181 PSPNRPPPSSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRA---- 240
           P P  PPPS             ST+D  F    +P P  S  E++   T    R A    
Sbjct: 181 PPP--PPPS-------------STWD--FWDPFIPPPPSSSEEEWEEETTTATRTATGTG 240

Query: 241 ------AAEKSGEEPPSSSAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFL 300
                  A  +     SS     SK        GS     + +   +L++I  E+D++FL
Sbjct: 241 SDAAVTTAPTTATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFL 300

Query: 301 KASESAHDVSKMLE----ATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDL 360
           KA++S   +S +LE     T    HS         ++   +     W R F     ++  
Sbjct: 301 KAADSGAPLSSLLEISTSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFAPSKLSEYR 360

Query: 361 NDDFDTEEN---ETHATVLDKLLAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNF 420
           N       N    +H++ +D+L AWEKKL++EVK  E +K +++KKV  + +L+ K + +
Sbjct: 361 NAGGVIGGNCIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEY 420

Query: 421 EAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFH 480
              EKAK  V  L ++  V  Q++ S  +EI ++R+ +LYP+LV+LV G+  MW  M+  
Sbjct: 421 VKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYES 480

Query: 481 HGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALS 540
           H  Q  +V  L+ L+   S + TS+ H + T+QL   VQ+WH     LV  Q+DYI++L+
Sbjct: 481 HQVQTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLT 540

Query: 541 NWLRLNLIPTESSLKEKVSSPPRVRS---PPIQSLLHVWQDHLEKLPDEVLRNSIFTFAT 600
            WLRL+L         + S  P VRS     I S    W   ++++PD+V    I +F T
Sbjct: 541 GWLRLSLF--------QFSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLT 600

Query: 601 VIRTIMQSQEEEMKLKVKCQETEKELARKSKQFKDWQKKYVQRRASNADESNLEETSDKD 660
            +  I+  Q +E K K + +   K+  +KS   +  + KY           ++ E+  K+
Sbjct: 601 AVHGIVAQQADEHKQKKRTESMLKDFEKKSASLRALESKY--------SPYSVPESRKKN 630

Query: 661 AIAERQAAVEAMEKRLEEEREEHQKLCLHVREKSLGSLKNQLPELFRALFEFSLACSR-- 701
            + E++  VE ++ + EEE+ +H+K     R  +L +L+   P +F+A+  FS  C +  
Sbjct: 661 PVIEKRVKVEMLKGKAEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAF 630

BLAST of HG10023340 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 8.2e-57
Identity = 218/762 (28.61%), Postives = 355/762 (46.59%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGC+ SK+E E+ V RCK+R+  MK+AVA+R   A+AH+ Y  SL+ T A LS +A G  
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  PPAPSSLPGAAVAQSAAAA-------------AYNSLPPPPPPLP-----GSPGMPLQ-- 120
             A S      +  +AA A             A +SLPPP P LP       P  P Q  
Sbjct: 61  SLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQSH 120

Query: 121 QATSMFEIKASKVEPKRVE-PVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGE 180
           Q      ++A +  P+R++ P I          + SV    R    +   G    +   +
Sbjct: 121 QPPPPVAVRAPRGGPRRLKVPHILS--------DSSVASPARSSFRKPVVGTPSSSSAWD 180

Query: 181 LAEEENGPPPPLPPS---PNRPPPSSENRRVPAPSPQDSTYDYLF--------------- 240
               EN  PP  P S     R     E  R+     ++    YL                
Sbjct: 181 W---ENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDD 240

Query: 241 -SVENMPAPTLSGVEDFSTNTEAIERR--------------AAAEKSGEEPPSSSAG--- 300
              E M        +D   +T   E R              AA  + G   PS  A    
Sbjct: 241 EREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPL 300

Query: 301 ----KTSKKMKQVGFPGS----SEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKML 360
               +   +  + G   S    +   R+V  +  L +I   ++++F+KA+E+ + VS++L
Sbjct: 301 PLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSELL 360

Query: 361 EATRLHFHSNFADNRGHIDHSARVMRVI--TW-NRSFRGLPNNDDLND-DFDTEENETHA 420
           EA+R     NF   +  + HS  ++  +  TW ++    +    D N  + ++ E ++H 
Sbjct: 361 EASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEMESMEGKSHG 420

Query: 421 TVLDKLLAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTR 480
           + L++LLAWEKKL++EVKA E +K E++KK++ L  L+ +G +   ++K KA+++ L + 
Sbjct: 421 STLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQSL 480

Query: 481 YIVDMQSMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDI 540
            IV  Q+  +T S I R+RD +L P+LV+L   + SMW  M+  H  Q ++V  +R L  
Sbjct: 481 IIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIVQQVRGLVD 540

Query: 541 SQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKE 600
           +   + TSD H   T  L A V  WHS   +L+  Q+DYI+AL  WL+L L   +S++ +
Sbjct: 541 NSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTLFQVDSNIPQ 600

Query: 601 KVSSPPRVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKC 660
           +  +   + S  + +    W+  L++LPD     +I +F  V+  I   Q EEMK+K + 
Sbjct: 601 EAYT--SLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIYTKQAEEMKIKKRT 660

Query: 661 QETEKELARKSKQFKDWQKKYVQRRA--------SNADESNLEETSDKDAIAERQAAVEA 686
           +   KEL +K+   +  +KKY Q  +        S  D         +D +AE++  +  
Sbjct: 661 ETYSKELEKKTNSLRAIEKKYYQSYSMVGLGLPGSGRDGIESHSFDARDPLAEKKTEIAQ 720

BLAST of HG10023340 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 5.5e-45
Identity = 201/799 (25.16%), Postives = 354/799 (44.31%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC+ SK++NE+ V RCKDR+  MK+AV AR+  AAAH+ Y  SL+ TG+ LS +A GE 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------------GPPAPSSLPGAAVAQ--SAAAAAYNSLPPPPPPLPGSPGMPLQQA 120
                           PP     P   V    S + A  +  PP   P   S   P   +
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 TSMFEIKASKVEPKRVEPVIEEVDENDFEIECS-VGPLRRRKGNRDGGGRSGRTGPGELA 180
           TS    +  + +P+    + E    +    E S   P       ++    +  +    + 
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 EEENGPPPPLPPSP--NRPPPSSENRRVPAPSPQD-----STYDYLFSVENMPAPTLSGV 240
             EN  PP  P S   NR     ++      + +D     S YD+ F            +
Sbjct: 181 NWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDF-FDTRKQKQKQFESM 240

Query: 241 -----EDFSTNTEAIE--------------RRAAAEKSGEEPPSSSAGKTSKK------- 300
                E+  T  E ++                 AAE+  E+    S  +   +       
Sbjct: 241 RNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTV 300

Query: 301 --------------MKQVGFPGSSEGK-----------------------RIVKGNINLL 360
                         M QV + G+ + K                       ++V  + +L 
Sbjct: 301 RSNSMRRHHQQPSPMPQV-YGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRHRDLK 360

Query: 361 QIFMELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVI--TW-NRS 420
           +I   + ++F KA+ S   VS+MLE  R     +F+  +  + HS+ ++  +  TW ++ 
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKKTVIHSSSLLSNLSSTWTSKP 420

Query: 421 FRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEVMKFEYQKKVAALNKL 480
              +    D         +++  + LD+LLAWEKKL+EE+KA E  K E++KK++ L   
Sbjct: 421 PLAVKYRIDTTALDQPNSSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQ 480

Query: 481 KKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVQLVNGMASM 540
           + KG +   ++K KA+++ L +  IV  Q++ +T + I R+RD  L P+LV+L +G   M
Sbjct: 481 EYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYM 540

Query: 541 WEIMHFHHGSQLKVVAALR-MLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQ 600
           W+ MH +H +Q  +V  +R +++ S   + TS+ H + T  L + V  WHS    L+  Q
Sbjct: 541 WKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHSSFSSLIKFQ 600

Query: 601 KDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHVWQDHLEKLPDEVLRNSI 660
           +D+I ++  W +L L+P    + ++ ++          +    W+  L+++PD V   +I
Sbjct: 601 RDFIHSVHAWFKLTLLP----VCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTVASEAI 660

Query: 661 FTFATVIRTIMQSQEEEMKLKVKCQETEKELARKSKQFKDWQKKYVQRRA------SNAD 693
            +F  V+  I   Q +E K+K + +   KEL +K+   ++ ++KY Q  +        + 
Sbjct: 661 KSFINVVHVISAKQADEHKIKKRTESASKELEKKASSVRNLERKYYQSYSMVGVGLPESG 720

BLAST of HG10023340 vs. ExPASy TrEMBL
Match: A0A1S4E3Q8 (uncharacterized protein LOC103500575 OS=Cucumis melo OX=3656 GN=LOC103500575 PE=4 SV=1)

HSP 1 Score: 1261.9 bits (3264), Expect = 0.0e+00
Identity = 659/717 (91.91%), Postives = 676/717 (94.28%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG+AV QSA AA YNSLPPPPPPLPGSPGM L       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GRTGPGELAEEENGPP P        P 
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPF-------PA 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           S E+RRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEEPPSS
Sbjct: 181 SGESRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEEPPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLVQL+NGMASMWE MHFHHGSQLKVVAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQSLLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQSLLHAWQDHLEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE  DKDAIAERQAAVEA+EKRLEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN  Q+QT+ QGVGT
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQGVGT 703

BLAST of HG10023340 vs. ExPASy TrEMBL
Match: A0A0A0K7A3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446740 PE=4 SV=1)

HSP 1 Score: 1260.4 bits (3260), Expect = 0.0e+00
Identity = 655/714 (91.74%), Postives = 675/714 (94.54%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG++V QSAAAA YNSLPPPPPPLPGSPGMPL       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSSVVQSAAAAGYNSLPPPPPPLPGSPGMPL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GR GPGELAEEENGPPPP        PP
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRAGPGELAEEENGPPPPF-------PP 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           SSENRRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEEPPSS
Sbjct: 181 SSENRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAATEKSGEEPPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKG+INLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGSINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLND FDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDGFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLV L+NGMASMWE MHFHHGSQLK VAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVHLINGMASMWETMHFHHGSQLKAVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQ LLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQILLHAWQDHLEKLPDEVLRNAIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE +DKDAIAERQAAVEA+EK+LEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPADKDAIAERQAAVEAVEKKLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQG 715
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN PQ+QT+ QG
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPMPNGPQNQTTTQG 700

BLAST of HG10023340 vs. ExPASy TrEMBL
Match: A0A6J1GQ84 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111456482 PE=4 SV=1)

HSP 1 Score: 1259.6 bits (3258), Expect = 0.0e+00
Identity = 667/720 (92.64%), Postives = 683/720 (94.86%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMK+AVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGAAVAQSAAAAA---YNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKR 120
           PPA SSLPG AVAQSAA AA   YNSLPPPPPPLPGSPGMPL+  TSMFEIKASKVEPKR
Sbjct: 61  PPAASSLPGVAVAQSAAVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVEPKR 120

Query: 121 VEPVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNR 180
           VE VIEEVDENDFEIECSVGPLRRR  NR+GGGR GRTG GELAEEENGPPPPLPPS NR
Sbjct: 121 VETVIEEVDENDFEIECSVGPLRRR-SNREGGGRGGRTGLGELAEEENGPPPPLPPSLNR 180

Query: 181 PPPSSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEP 240
           PPP +ENRR  +PSPQD+TYDYLFSVENMPAPTLS VEDF TNTEAIERRAA EKSG E 
Sbjct: 181 PPPPNENRRAHSPSPQDATYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAVEKSGGEL 240

Query: 241 PSSSAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVGFP S EGKR VKGN +LLQIFMELDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHG QLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGGQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKE 600
           RVRSPPIQSLLHVWQDHLEKLPDEVLRN+IFTFATVI TI+QSQEEEMKLKVKCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKVKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRRA NADE+N EET DKDAIAERQAAVEA+EKRLEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNADEANQEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQT+A+ VGT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTTAR-VGT 718

BLAST of HG10023340 vs. ExPASy TrEMBL
Match: A0A5A7UW13 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G00130 PE=4 SV=1)

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 658/717 (91.77%), Postives = 675/717 (94.14%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVVSRCKDRKMFMKDAV ARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG
Sbjct: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVTARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60

Query: 61  PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKRVEP 120
           PPAPSSLPG+AV QSA AA YNSLPPPPPPLPGSPGM L       EIKASKVEPKRVEP
Sbjct: 61  PPAPSSLPGSAVVQSAVAAGYNSLPPPPPPLPGSPGMSL-------EIKASKVEPKRVEP 120

Query: 121 VIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNRPPP 180
           VI+EVDENDFEIECSVGPLRRR+ NRDG GR GRTGPGELAEEENGPP P        P 
Sbjct: 121 VIQEVDENDFEIECSVGPLRRRRSNRDGSGRGGRTGPGELAEEENGPPLPF-------PA 180

Query: 181 SSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEPPSS 240
           S E+RRVP PSPQDSTYDYLFSV+NMPAPTLSGVEDF  NTE +ERRAA EKSGEE PSS
Sbjct: 181 SGESRRVPVPSPQDSTYDYLFSVDNMPAPTLSGVEDFGANTETVERRAAMEKSGEELPSS 240

Query: 241 SAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300
           SAGKTSKKMKQVG+PGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL
Sbjct: 241 SAGKTSKKMKQVGYPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRL 300

Query: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360
           HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW
Sbjct: 301 HFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAW 360

Query: 361 EKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420
           EKKLFEEVKAGE+MKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD
Sbjct: 361 EKKLFEEVKAGEIMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMD 420

Query: 421 STVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSD 480
           STVSEINRIRDEQLYPKLVQL+NGMASMWE MHFHHGSQLKVVAALRMLDISQSPKETSD
Sbjct: 421 STVSEINRIRDEQLYPKLVQLINGMASMWETMHFHHGSQLKVVAALRMLDISQSPKETSD 480

Query: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540
           HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR
Sbjct: 481 HHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVR 540

Query: 541 SPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELAR 600
           SPPIQSLLH WQDHLEKLPDEVLRN+IFTFATVI TIMQSQEEEMKLK+KCQETEKELAR
Sbjct: 541 SPPIQSLLHAWQDHLEKLPDEVLRNTIFTFATVIHTIMQSQEEEMKLKLKCQETEKELAR 600

Query: 601 KSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCL 660
           KSKQFKDWQKKYVQRR SNADE ++EE  DKDAIAERQAAVEA+EKRLEEEREEHQKLCL
Sbjct: 601 KSKQFKDWQKKYVQRRGSNADEVDMEEPGDKDAIAERQAAVEAVEKRLEEEREEHQKLCL 660

Query: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQP+PN  Q+QT+ QGVGT
Sbjct: 661 HVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPIPNGSQNQTTTQGVGT 703

BLAST of HG10023340 vs. ExPASy TrEMBL
Match: A0A6J1JYE9 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111488577 PE=4 SV=1)

HSP 1 Score: 1251.9 bits (3238), Expect = 0.0e+00
Identity = 663/720 (92.08%), Postives = 683/720 (94.86%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGCSQSKIENEEVV RCKDRKMFMK+AVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE 
Sbjct: 1   MGCSQSKIENEEVVCRCKDRKMFMKEAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEV 60

Query: 61  PPAPSSLPGAAVAQS---AAAAAYNSLPPPPPPLPGSPGMPLQQATSMFEIKASKVEPKR 120
           PPA SSLPG AVAQS   AA+A+YNSLPPPPPPLPGSPGMPL+  TSMFEIKASKVE KR
Sbjct: 61  PPAASSLPGVAVAQSAVVAASASYNSLPPPPPPLPGSPGMPLRNGTSMFEIKASKVESKR 120

Query: 121 VEPVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEENGPPPPLPPSPNR 180
           VEPVIEEVDENDFEIECSVGPLRRR  NR+GGGR  RTG GELAEEENGPPPPLPPS N 
Sbjct: 121 VEPVIEEVDENDFEIECSVGPLRRR-SNREGGGRGSRTGLGELAEEENGPPPPLPPSLNL 180

Query: 181 PPPSSENRRVPAPSPQDSTYDYLFSVENMPAPTLSGVEDFSTNTEAIERRAAAEKSGEEP 240
           PPP +ENRRV +PSPQDSTYDYLFSVENMPAPTLS VEDF TNTEAIERRAAAE+SG E 
Sbjct: 181 PPPPNENRRVHSPSPQDSTYDYLFSVENMPAPTLSSVEDFGTNTEAIERRAAAEESGREL 240

Query: 241 PSSSAGKTSKKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEA 300
           PSSSAGKTSKK+KQVGFP S EGKR VKGN +LLQIFMELDDHFLKASESAHDVSKMLEA
Sbjct: 241 PSSSAGKTSKKLKQVGFPCSIEGKRAVKGNTSLLQIFMELDDHFLKASESAHDVSKMLEA 300

Query: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKL 360
           TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEE+ETHATVLDKL
Sbjct: 301 TRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEEHETHATVLDKL 360

Query: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420
           LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ
Sbjct: 361 LAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQ 420

Query: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKE 480
           SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLK VAALR LDI QSPKE
Sbjct: 421 SMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKAVAALRTLDIPQSPKE 480

Query: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPP 540
           TSDHHHERTVQLWAVVQEWHSQLEKLV RQK+YIKALSNWLRLNLIPTESSLKEKVSSPP
Sbjct: 481 TSDHHHERTVQLWAVVQEWHSQLEKLVTRQKEYIKALSNWLRLNLIPTESSLKEKVSSPP 540

Query: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKE 600
           RVRSPPIQSLLHVWQDHLEKLPDEVLRN+IFTFATVI TI+QSQEEEMKLK KCQETEKE
Sbjct: 541 RVRSPPIQSLLHVWQDHLEKLPDEVLRNAIFTFATVINTIVQSQEEEMKLKAKCQETEKE 600

Query: 601 LARKSKQFKDWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQK 660
           LARKSKQFKDWQKKYVQRRA N++E+N EET DKDAIAERQAAVEA+EKRLEEEREEHQK
Sbjct: 601 LARKSKQFKDWQKKYVQRRAPNSNEANPEETGDKDAIAERQAAVEAVEKRLEEEREEHQK 660

Query: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQGVGT 718
           LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQT+A+ VGT
Sbjct: 661 LCLHVREKSLGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTTAR-VGT 718

BLAST of HG10023340 vs. TAIR 10
Match: AT1G52320.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF630 (InterPro:IPR006868), Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 8725 Blast hits to 7476 proteins in 620 species: Archae - 10; Bacteria - 622; Metazoa - 3286; Fungi - 1319; Plants - 1442; Viruses - 221; Other Eukaryotes - 1825 (source: NCBI BLink). )

HSP 1 Score: 664.5 bits (1713), Expect = 9.9e-191
Identity = 404/800 (50.50%), Postives = 506/800 (63.25%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGE- 60
           MGC+QSKIENEE V+RCK+RK  MKDAV ARNAFAAAHS+YAM+LKNTGA LSDY+HGE 
Sbjct: 1   MGCAQSKIENEEAVTRCKERKQLMKDAVTARNAFAAAHSAYAMALKNTGAALSDYSHGEF 60

Query: 61  ------------------------GPPAPSS---LPGAAVAQSAAAAAY---NSLPPPPP 120
                                    PP PSS   +  +  + S+AA      ++LPPPPP
Sbjct: 61  LVSNHSSSSAAAAIASTSSLPTAISPPLPSSTAPVSNSTASSSSAAVPQPIPDTLPPPPP 120

Query: 121 PLPGSPGMPLQQATSMFEIK--------ASKVEPKRVEPVIEEVDENDFEIECSV----G 180
           P    P +PLQ+A +M E+          S +     +  ++  D++D + + S      
Sbjct: 121 P----PPLPLQRAATMPEMNGRSGGGHAGSGLNGIEEDGALDNDDDDDDDDDDSEMENRD 180

Query: 181 PLRRRKGNRDGGGRSGRT--GPGELAEEENGPPPPLPPSPNRPPPSSENRRVPAPSPQDS 240
            L R+  +R G  R  RT      L EE+  PPPPL  S   PPP  +++       Q  
Sbjct: 181 RLIRKSRSRGGSTRGNRTTIEDHHLQEEKAPPPPPLANSRPIPPP-RQHQHQHQQQQQQP 240

Query: 241 TYDYLF-SVENMPAPTLSGV---------------------------------EDFSTNT 300
            YDY F +VENMP  TL                                    E+     
Sbjct: 241 FYDYFFPNVENMPGTTLEDTPPQPQPQPTRPVPPQPHSPVVTEDDEDEEEEEEEEEEEEE 300

Query: 301 EAIERRAAAE---KSGEEPPSSSAGKTS----KKMKQVGFPGSSEGKRIVKGNINLLQIF 360
             IER+   E   K  EE        T+    KK K +G PG   G R+     +L  +F
Sbjct: 301 TVIERKPLVEERPKRVEEVTIELEKVTNLRGMKKSKGIGIPGERRGMRMPVTATHLANVF 360

Query: 361 MELDDHFLKASESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPN 420
           +ELDD+FLKASESAHDVSKMLEATRLH+HSNFADNRGHIDHSARVMRVITWNRSFRG+PN
Sbjct: 361 IELDDNFLKASESAHDVSKMLEATRLHYHSNFADNRGHIDHSARVMRVITWNRSFRGIPN 420

Query: 421 NDDLNDDFDTEENETHATVLDKLLAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSN 480
            DD  DD D EENETHATVLDKLLAWEKKL++EVKAGE+MK EYQKKVA LN++KK+G +
Sbjct: 421 ADDGKDDVDLEENETHATVLDKLLAWEKKLYDEVKAGELMKIEYQKKVAHLNRVKKRGGH 480

Query: 481 FEAIEKAKATVSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHF 540
            +++E+AKA VSHLHTRYIVDMQSMDSTVSEINR+RDEQLY KLV LV  M  MWE+M  
Sbjct: 481 SDSLERAKAAVSHLHTRYIVDMQSMDSTVSEINRLRDEQLYLKLVHLVEAMGKMWEMMQI 540

Query: 541 HHGSQLKVVAALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKAL 600
           HH  Q ++   LR LD+SQ+ KET+DHHHERT+QL AVVQEWH+Q  ++++ QK+YIKAL
Sbjct: 541 HHQRQAEISKVLRSLDVSQAVKETNDHHHERTIQLLAVVQEWHTQFCRMIDHQKEYIKAL 600

Query: 601 SNWLRLNLIPTESSLKEKVSSPPRVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVI 660
             WL+LNLIP ES+LKEKVSSPPRV +P IQ LLH W D L+K+PDE+ +++I  FA V+
Sbjct: 601 GGWLKLNLIPIESTLKEKVSSPPRVPNPAIQKLLHAWYDRLDKIPDEMAKSAIINFAAVV 660

Query: 661 RTIMQSQEEEMKLKVKCQETEKELARKSKQFKDWQKKYVQRRASNADESNLEETSDKDAI 715
            TIMQ QE+E+ L+ KC+ET KEL RK +QF+DW  KY+Q+R       +  +    D +
Sbjct: 661 STIMQQQEDEISLRNKCEETRKELGRKIRQFEDWYHKYIQKRGPEGMNPDEADNDHNDEV 720

BLAST of HG10023340 vs. TAIR 10
Match: AT5G25590.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 594.7 bits (1532), Expect = 9.6e-170
Identity = 367/778 (47.17%), Postives = 481/778 (61.83%), Query Frame = 0

Query: 1   MGCSQSKIENEEVVSRCKDRKMFMKDAVAARNAFAAAHSSYAMSLKNTGAVLSDYAHGEG 60
           MGC+QS+++NEE V+RCK+R+  +K+AV+A  AFAA H +YA++LKNTGA LSDY HGE 
Sbjct: 1   MGCAQSRVDNEEAVARCKERRNVIKEAVSASKAFAAGHFAYAIALKNTGAALSDYGHGES 60

Query: 61  ---------------PPAPSSLPGAAVAQSAAAAAYNSLPPPPPPLPGSPGMPLQQATSM 120
                               +    A  Q        +LPPPPPPLP     P+++A S+
Sbjct: 61  DQKALDDVLLDQQHYEKQSRNNVDPASPQPPPPPPIENLPPPPPPLPKFSPSPIKRAISL 120

Query: 121 FEIKASKVEPKRVEPVIEEVDENDFEIECSVGPLRRRKGNRDGGGRSGRTGPGELAEEEN 180
             +     + + ++ +  E +E D E E  V      KG       SGR    E  EEE 
Sbjct: 121 PSMAVRGRKVQTLDGMAIEEEEEDEEEEEEV------KG-------SGRDTAQE--EEEP 180

Query: 181 GPPPPLPPSPNRPPPSSENRRVPAPSPQDS-TYDYLFSVENMPAPTL------SGVED-- 240
             P  +  S  R         + + SP +S  +DY F VENMP P L      +G E+  
Sbjct: 181 RTPENVGKSNGRKRLEKTTPEIVSASPANSMAWDYFFMVENMPGPNLDDREVRNGYENQS 240

Query: 241 --FSTNTEAIERRAAAEKSGEEPPSSSAGKTSKKMKQ----------------------- 300
             F  N E  E     E+SG     S +GK  ++M+                        
Sbjct: 241 SHFQFNEEDDEEEEEEERSGIYRKKSGSGKVVEEMEPKTPEKVEEEEEEDEEEDEEEEEE 300

Query: 301 ------VGFPGSSEGKRIVK---------------------GNINLLQIFMELDDHFLKA 360
                 V      +GK  ++                      ++NL++I  E+DD FLKA
Sbjct: 301 EEEEVVVEVKKKKKGKAKIEHSSTAPPEFRRAVAKTSAAASSSVNLMKILDEIDDRFLKA 360

Query: 361 SESAHDVSKMLEATRLHFHSNFADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDT 420
           SE A +VSKMLEATRLH+HSNFADNRG++DHSARVMRVITWN+S RG+ N +   DD ++
Sbjct: 361 SECAQEVSKMLEATRLHYHSNFADNRGYVDHSARVMRVITWNKSLRGISNGEGGKDDQES 420

Query: 421 EENETHATVLDKLLAWEKKLFEEVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKAT 480
           +E+ETHATVLDKLLAWEKKL++EVK GE+MK EYQKKV+ LN+ KK+G++ E +EK KA 
Sbjct: 421 DEHETHATVLDKLLAWEKKLYDEVKQGELMKIEYQKKVSLLNRHKKRGASAETVEKTKAA 480

Query: 481 VSHLHTRYIVDMQSMDSTVSEINRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVA 540
           VSHLHTRYIVDMQSMDSTVSE+NR+RD+QLYP+LV LV GMA MW  M  HH +QL +V 
Sbjct: 481 VSHLHTRYIVDMQSMDSTVSEVNRLRDDQLYPRLVALVEGMAKMWTNMCIHHDTQLGIVG 540

Query: 541 ALRMLDISQSPKETSDHHHERTVQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIP 600
            L+ L+IS S KET+  HH +T Q   V++EWH Q + LV  QK YI +L+NWL+LNLIP
Sbjct: 541 ELKALEISTSLKETTKQHHHQTRQFCTVLEEWHVQFDTLVTHQKQYINSLNNWLKLNLIP 600

Query: 601 TESSLKEKVSSPPRVRSPPIQSLLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEE 660
            ESSLKEKVSSPPR + PPIQ+LLH W D LEKLPDEV +++I +FA VI+TI+  QEEE
Sbjct: 601 IESSLKEKVSSPPRPQRPPIQALLHSWHDRLEKLPDEVAKSAISSFAAVIKTILLHQEEE 660

Query: 661 MKLKVKCQETEKELARKSKQFKDWQKKYVQRR--ASNADESNLEETSDKDAIAERQAAVE 701
           MKLK KC+ET +E  RK + F+DW +K++Q+R     A+  +   TS +D + ER+ AVE
Sbjct: 661 MKLKEKCEETRREFIRKKQGFEDWYQKHLQKRGPTEEAEGGDDATTSSRDHVTERRIAVE 720

BLAST of HG10023340 vs. TAIR 10
Match: AT1G52320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 517 Blast hits to 513 proteins in 62 species: Archae - 6; Bacteria - 6; Metazoa - 50; Fungi - 2; Plants - 427; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink). )

HSP 1 Score: 569.7 bits (1467), Expect = 3.3e-162
Identity = 290/468 (61.97%), Postives = 358/468 (76.50%), Query Frame = 0

Query: 247 KKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNF 306
           KK K +G PG   G R+     +L  +F+ELDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 307 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFE 366
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  DD D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 367 EVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 426
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 427 NRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 486
           NR+RDEQLY KLV LV  M  MWE+M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 487 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQS 546
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 547 LLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELARKSKQFK 606
           LLH W D L+K+PDE+ +++I  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 607 DWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCLHVREKS 666
           DW  KY+Q+R       +  +    D +A RQ  VE ++KRLEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 667 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQG 715
           L SL+ +LPELF+A+ E + +CS MYR +   S+      + Q  +QG
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469

BLAST of HG10023340 vs. TAIR 10
Match: AT1G52320.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 569.7 bits (1467), Expect = 3.3e-162
Identity = 290/468 (61.97%), Postives = 358/468 (76.50%), Query Frame = 0

Query: 247 KKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNF 306
           KK K +G PG   G R+     +L  +F+ELDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 307 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFE 366
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  DD D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 367 EVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 426
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 427 NRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 486
           NR+RDEQLY KLV LV  M  MWE+M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 487 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQS 546
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 547 LLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELARKSKQFK 606
           LLH W D L+K+PDE+ +++I  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 607 DWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCLHVREKS 666
           DW  KY+Q+R       +  +    D +A RQ  VE ++KRLEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 667 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQG 715
           L SL+ +LPELF+A+ E + +CS MYR +   S+      + Q  +QG
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469

BLAST of HG10023340 vs. TAIR 10
Match: AT1G52320.4 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF632 (InterPro:IPR006867); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF630 and DUF632) (TAIR:AT5G25590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 569.7 bits (1467), Expect = 3.3e-162
Identity = 290/468 (61.97%), Postives = 358/468 (76.50%), Query Frame = 0

Query: 247 KKMKQVGFPGSSEGKRIVKGNINLLQIFMELDDHFLKASESAHDVSKMLEATRLHFHSNF 306
           KK K +G PG   G R+     +L  +F+ELDD+FLKASESAHDVSKMLEATRLH+HSNF
Sbjct: 2   KKSKGIGIPGERRGMRMPVTATHLANVFIELDDNFLKASESAHDVSKMLEATRLHYHSNF 61

Query: 307 ADNRGHIDHSARVMRVITWNRSFRGLPNNDDLNDDFDTEENETHATVLDKLLAWEKKLFE 366
           ADNRGHIDHSARVMRVITWNRSFRG+PN DD  DD D EENETHATVLDKLLAWEKKL++
Sbjct: 62  ADNRGHIDHSARVMRVITWNRSFRGIPNADDGKDDVDLEENETHATVLDKLLAWEKKLYD 121

Query: 367 EVKAGEVMKFEYQKKVAALNKLKKKGSNFEAIEKAKATVSHLHTRYIVDMQSMDSTVSEI 426
           EVKAGE+MK EYQKKVA LN++KK+G + +++E+AKA VSHLHTRYIVDMQSMDSTVSEI
Sbjct: 122 EVKAGELMKIEYQKKVAHLNRVKKRGGHSDSLERAKAAVSHLHTRYIVDMQSMDSTVSEI 181

Query: 427 NRIRDEQLYPKLVQLVNGMASMWEIMHFHHGSQLKVVAALRMLDISQSPKETSDHHHERT 486
           NR+RDEQLY KLV LV  M  MWE+M  HH  Q ++   LR LD+SQ+ KET+DHHHERT
Sbjct: 182 NRLRDEQLYLKLVHLVEAMGKMWEMMQIHHQRQAEISKVLRSLDVSQAVKETNDHHHERT 241

Query: 487 VQLWAVVQEWHSQLEKLVNRQKDYIKALSNWLRLNLIPTESSLKEKVSSPPRVRSPPIQS 546
           +QL AVVQEWH+Q  ++++ QK+YIKAL  WL+LNLIP ES+LKEKVSSPPRV +P IQ 
Sbjct: 242 IQLLAVVQEWHTQFCRMIDHQKEYIKALGGWLKLNLIPIESTLKEKVSSPPRVPNPAIQK 301

Query: 547 LLHVWQDHLEKLPDEVLRNSIFTFATVIRTIMQSQEEEMKLKVKCQETEKELARKSKQFK 606
           LLH W D L+K+PDE+ +++I  FA V+ TIMQ QE+E+ L+ KC+ET KEL RK +QF+
Sbjct: 302 LLHAWYDRLDKIPDEMAKSAIINFAAVVSTIMQQQEDEISLRNKCEETRKELGRKIRQFE 361

Query: 607 DWQKKYVQRRASNADESNLEETSDKDAIAERQAAVEAMEKRLEEEREEHQKLCLHVREKS 666
           DW  KY+Q+R       +  +    D +A RQ  VE ++KRLEEE E + +    VREKS
Sbjct: 362 DWYHKYIQKRGPEGMNPDEADNDHNDEVAVRQFNVEQIKKRLEEEEEAYHRQSHQVREKS 421

Query: 667 LGSLKNQLPELFRALFEFSLACSRMYRHLKSISQPLPNRPQSQTSAQG 715
           L SL+ +LPELF+A+ E + +CS MYR +   S+      + Q  +QG
Sbjct: 422 LASLRTRLPELFQAMSEVAYSCSDMYRAITYASKRQSQSERHQKPSQG 469