HG10023318 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023318
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAcyl-[acyl-carrier-protein] hydrolase
LocationChr05: 33009432 .. 33012367 (-)
RNA-Seq ExpressionHG10023318
SyntenyHG10023318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGCAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTAAGATTAAAAATTCTTTCTTTTTTCCAACACTTTTGTTTGTGTTTTTTTGAAAGATATAATTGATATATATATTTTTGGCAGGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGTCAAATCCTACGAGTCGGGACCAGATAAAAATATGACAATGTTTTTCATCATCAACCAGTTACAGGTTAGAAATTGTAACAGTTGTAGGTTTAATATTTATGTTTTGAGGATTCAATTTTTACCACTATTGTTAGTTTGAGGGTCTAATTTTAACACTTTGTAAGTTTGAGAGTGCAATTTTTACTACCACCATACTTTAGGATTTTTTTTTTTTTTTTTTTTTTTGCAATTTATACTTTATTTTTATTTATTAATTTATTTATTGGTATTAATTTAATTTATCCTTTATTTTCATTAGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGTAATTACATTTTTTTCCTTGAAATTGCATAATTTAACAAGTCACTTTTTTTTATAAAAAAATAAATAATTTATGGTATTTTTGTATTATATATCTATGTTTGTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGAATGATGTTGTTCAACTTCGAACATGGGGTTGTGTTCATGGGAAGAATAGTTATCGTCGAGATTGGGTTGTTCGCAACTATAAAACTGGTCAAGTTTTGATTCGTGCTACTTGGTAATTTGTTTGTTTGTTTTTTTTTAAAAAAAAATCAAGTTTGTTCACTAATTTGTCTGTGTTTTGTAGTTTCTACGTTTTGATGAACAGAAACACAAGAAAATTATCGAAATTTTGTGATGAAATGAAGGTGGAAATGAATCCCCACTTCTTGGATAGTGATCCAATCATTGAAGAAGATAACATCATCTCAACAAAACTAGATATTGATGCAAAAAATTGCAATTGCACATCTTTAGTGGTAAGTATAAATACAATTTTCAAAAATCAAAAATTAAATGATTATAAAAAAGAACGTCTTTCTTTTAATGTTTTCGAGAGAAAATAACTAATTTCTTTATTAATTTAAAACTTAACGTAGACATAATTACCATTGAAGATTTTTCAAAAGCTAAACATGAAAATTATCATTCGTATTATATTAAAAAAAACAAATAATAAAAAGACAGTTTGATAAATGAGTATCCATATAGATATATATTTAATGTTGTACATTAGATTTAGGGCTGTTTTCAAATATAGAAAAATGAAGTGATAGACAACGATAAAAGTTAATCACTCAAGCAAAAGAATTCTATCGCGGTCTATCATTGATAGACAATAAAATTTTTCTATATTTATAAATAATTTGATTTTTTTTTTCTGTTTATAGTAATTTCCCTTAGATTTAACCATATATTTGAATCCTTATTAAATGATTAATCTAACATTATTATCCATTAATACTAACACTTTCTTTTTTGTTAACCCCTCACCTTTCTACAAAAATTCAGACTAGATTGAGTGATTTGGATATGAACTACCATGTCAACAATGCTAGTTATATCAACTTCATCCTTGAGGTATGTTATTTTTTATCCTCTTTATTTACCTTCAATTGTTTCTTACAATAAATCAATAGAATAAAAATTAAACAAAAAGTGATATTCATGTATTTGAGTAATGAAAGTACTTCTAAATAGTGTATTACTAAGAGTAAAAATAAATTATTTTTCAAAAAAAAAAAAAAAAAACTAAGAGTGCATTAATTTAAAACTCACCCAATATAATAAGTTTCAAATTCTTTTATTGTGAATGTATTTAGTTATGGTGTGTTTGGAATATATTTTCAAGTGTTTAGTTTATGGCTAAATTTTTGGCCCCCCACTTAAATACAAATTTAAAAGAGACTAGTTTCAAATAAAACAAAATGAGTTAACTTATTTATAAATATAGTAAAAGGGTTTGTTTTCAAATATATCATTTATTAGTGTCTATCAGTGATGGACTGTGATAGATAGTGGCATTTTGCTATGTTTAAAAATATTTCCAACAGTTTTGCCATTTAAAATAATTACGCAACTTAAAAGAAAAAAAGCCTGAAGCCAAAATAAGAAATACAAGAAAAATATGTCTAAAATGAAAATAAAAGAAAACACACAAAAATTTACTTAAAAAAAAAATCAGAACCAAAGAAAAATCATAACCCACAAATATATATATTACCATCATTCTACCGATTTCAATTACAATAACATTCTCTCAAACTCAGTTTTTTAATTGAGATAATTGCTCTTTTAATATTTTGTGAAAACGTTACAATATAGTCAACATTTATAACAATGGATGACAAAAGAGAATTGTAAAAAATATTATGGTTTCAGGGAACTCCTCGATGTGTGTCGGAGAATCATCGGCTCTCAGCTGTAACAATCAAATACCTTAAAGAATGTGAAGTGGATAAACAGTTGCAATCTCTGTCTATGCCTATTAAAAACGACAACGTTTTGAATGATAGCCATCTCCAACTCCACCACTCGGTTCTCGCTGATGAGATTGAACTTGTTCGAGCAACAACCCTTTGGACACCAAAATCCATTTAA

mRNA sequence

ATGATGGCAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGTCAAATCCTACGAGTCGGGACCAGATAAAAATATGACAATGTTTTTCATCATCAACCAGTTACAGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGAATGATGTTGTTCAACTTCGAACATGGGGTTGTGTTCATGGGAAGAATAGTTATCGTCGAGATTGGGTTGTTCGCAACTATAAAACTGGTCAAGTTTTGATTCGTGCTACTTGTTTCTACGTTTTGATGAACAGAAACACAAGAAAATTATCGAAATTTTGTGATGAAATGAAGGTGGAAATGAATCCCCACTTCTTGGATAGTGATCCAATCATTGAAGAAGATAACATCATCTCAACAAAACTAGATATTGATGCAAAAAATTGCAATTGCACATCTTTAGTGACTAGATTGAGTGATTTGGATATGAACTACCATGTCAACAATGCTAGTTATATCAACTTCATCCTTGAGGGAACTCCTCGATGTGTGTCGGAGAATCATCGGCTCTCAGCTGTAACAATCAAATACCTTAAAGAATGTGAAGTGGATAAACAGTTGCAATCTCTGTCTATGCCTATTAAAAACGACAACGTTTTGAATGATAGCCATCTCCAACTCCACCACTCGGTTCTCGCTGATGAGATTGAACTTGTTCGAGCAACAACCCTTTGGACACCAAAATCCATTTAA

Coding sequence (CDS)

ATGATGGCAGCAATATCTTCATCTTATAGTTGTAATTTTGGTAAGAAAATGAATTCAATTTTGAAGAAAGAACAAAGATTTGGAGATACAAGTAGAGGCAATTTGCAGTTCAAAGGCCATCCCAAAATTTGCTTAGAAAGGCTCAAAACTTCTAACATTATTGCTAAGTCCACCATTGCAAGTGAGGTTAGAAAGATTGATAGTTATGAGTCTAGAAGTCTTGATATAACTCATGGGAACTTAACGATGGGAGAATTAGTGAACGATGGTTTGGTTTATCAACGCCATTTCTTGGTCAAATCCTACGAGTCGGGACCAGATAAAAATATGACAATGTTTTTCATCATCAACCAGTTACAGGAGTCTAAACTAAACCACTTGAAAAGTTGTGGATTGGTGACGACAGGATTGGGCACAACACCCGAAATGACCAAAAGGGGCCTCCTATGGGTGGTTCATAAATTGCAGGTGGTGGTGGACCAATATCCTTCTTGGAATGATGTTGTTCAACTTCGAACATGGGGTTGTGTTCATGGGAAGAATAGTTATCGTCGAGATTGGGTTGTTCGCAACTATAAAACTGGTCAAGTTTTGATTCGTGCTACTTGTTTCTACGTTTTGATGAACAGAAACACAAGAAAATTATCGAAATTTTGTGATGAAATGAAGGTGGAAATGAATCCCCACTTCTTGGATAGTGATCCAATCATTGAAGAAGATAACATCATCTCAACAAAACTAGATATTGATGCAAAAAATTGCAATTGCACATCTTTAGTGACTAGATTGAGTGATTTGGATATGAACTACCATGTCAACAATGCTAGTTATATCAACTTCATCCTTGAGGGAACTCCTCGATGTGTGTCGGAGAATCATCGGCTCTCAGCTGTAACAATCAAATACCTTAAAGAATGTGAAGTGGATAAACAGTTGCAATCTCTGTCTATGCCTATTAAAAACGACAACGTTTTGAATGATAGCCATCTCCAACTCCACCACTCGGTTCTCGCTGATGAGATTGAACTTGTTCGAGCAACAACCCTTTGGACACCAAAATCCATTTAA

Protein sequence

MMAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQSLSMPIKNDNVLNDSHLQLHHSVLADEIELVRATTLWTPKSI
Homology
BLAST of HG10023318 vs. NCBI nr
Match: KAA0058801.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 607.8 bits (1566), Expect = 5.8e-170
Identity = 296/360 (82.22%), Postives = 328/360 (91.11%), Query Frame = 0

Query: 2   MAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 61
           MAA+SSSY CN GKK NS LKKEQRFGD++ GNLQ K HPK+ LE L+TSNIIAKS+IAS
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 62  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 121
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFLVKSYESGPDK MTMFFIINQL E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 122 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKN 181
           SKLNHLKSCGLVTTGLGTTPEMTKRGL+WVVHKLQVVVDQYPSWND+VQL+TW C +GKN
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSWNDIVQLQTWSCFYGKN 180

Query: 182 SYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDN 241
           SYRRDW+VRNYKTGQVLIRA+CFYVLMNRNTRKLSKF DEMK EMNPHFL SDPIIEE+N
Sbjct: 181 SYRRDWIVRNYKTGQVLIRASCFYVLMNRNTRKLSKFYDEMKEEMNPHFLKSDPIIEEEN 240

Query: 242 IISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIK 301
           IISTKLD+DAK CNCTSLVTRLSDLDMN+HVNNASYINFILEGTPR + E+HRLS VT+K
Sbjct: 241 IISTKLDVDAKGCNCTSLVTRLSDLDMNHHVNNASYINFILEGTPRWMLESHRLSTVTVK 300

Query: 302 YLKECEVDKQLQSLSMPIKNDNVLN------DSHLQLHHSVLADEIELVRATTLWTPKSI 356
           YLKEC VDK LQSLSMPI+ND + N      +++++LHHS++++ IELVRATTLWTP SI
Sbjct: 301 YLKECGVDKILQSLSMPIQNDVISNGNYLVKENYIKLHHSIVSEGIELVRATTLWTPYSI 360

BLAST of HG10023318 vs. NCBI nr
Match: KAG6593401.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 452.6 bits (1163), Expect = 3.1e-123
Identity = 230/363 (63.36%), Postives = 273/363 (75.21%), Query Frame = 0

Query: 1   MMAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIA 60
           MMA I SS   +FG ++ SI  +E  FG  +  +L  KG PK     LK+S+I     IA
Sbjct: 9   MMALICSSNGSHFGMELVSISTREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIA 68

Query: 61  SEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQ 120
              + ID Y SR  +IT  NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQ
Sbjct: 69  GGFKSIDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQ 128

Query: 121 ESKLNHLKSCGLV-----TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWG 180
           ESKLNH  SCGLV      TG GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW 
Sbjct: 129 ESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWI 188

Query: 181 CVHGKNSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDP 240
           C HGKNSYRR+W+ R+ K+G+VLIRA CFYVLMN+ TRKLS+FC+E+K EMNP FLDS P
Sbjct: 189 CKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNKRTRKLSRFCEELKEEMNPFFLDSHP 248

Query: 241 IIEEDNIISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRL 300
           I+EEDNI+S KL      C   SLVTRLSDLDMN H+NNASYINF+LEGTPR V EN++L
Sbjct: 249 ILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINFVLEGTPRAVLENYQL 308

Query: 301 SAVTIKYLKECEVDKQLQSLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLW 354
           S +TIKYLKEC VD+QLQSLS+P++ND V     + D  ++LHHS+L+D IELVRA T+W
Sbjct: 309 SGITIKYLKECSVDQQLQSLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVW 368

BLAST of HG10023318 vs. NCBI nr
Match: XP_023514527.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 451.8 bits (1161), Expect = 5.4e-123
Identity = 229/362 (63.26%), Postives = 273/362 (75.41%), Query Frame = 0

Query: 2   MAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 61
           MA I SS   +FG ++ SI  +E  FG  +  +L  K  PK     LK+S+I     IA 
Sbjct: 1   MALICSSNGSHFGMELVSISMREPTFGHATTPSLHLKARPKTASLTLKSSHI--SQPIAG 60

Query: 62  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 121
             + ID Y SR  +IT  NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQE
Sbjct: 61  GFKSIDDYVSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQE 120

Query: 122 SKLNHLKSCGLV-----TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGC 181
           SKLNH  SCGLV      TG GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW C
Sbjct: 121 SKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWIC 180

Query: 182 VHGKNSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPI 241
            HGKNSYRR+W+ R+ K+G+VLIRA CFYVLMN+ TRKLSKFC+E+K EMNP FLDS PI
Sbjct: 181 KHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNKRTRKLSKFCEELKEEMNPFFLDSHPI 240

Query: 242 IEEDNIISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLS 301
           +EEDNI+S KL +   +C   SLVTRLSDLDMN H+NNASYINF+LEGTPR V EN++LS
Sbjct: 241 LEEDNIVSQKLHLHQHDCTHASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLS 300

Query: 302 AVTIKYLKECEVDKQLQSLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLWT 354
            +TIKYLKEC VD+QLQSLS+P++ND V     + D  ++LHHS+L+D IELVRA T+WT
Sbjct: 301 GITIKYLKECSVDQQLQSLSVPLENDVVNGTPFIKDKDIKLHHSILSDGIELVRAKTVWT 360

BLAST of HG10023318 vs. NCBI nr
Match: XP_023004418.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 441.4 bits (1134), Expect = 7.2e-120
Identity = 222/345 (64.35%), Postives = 265/345 (76.81%), Query Frame = 0

Query: 19  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 78
           SI  +E  FG  +  +L  KG PK     LK+S+I     IAS  + +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIASGFKSMDDYVSRKPEITR 64

Query: 79  GNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGL- 138
            NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQESKLNH  SCGLV TGL 
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTGLG 124

Query: 139 ----GTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYK 198
               GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW C HGKNSYRR+W+ R+ K
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICRHGKNSYRRNWIARDKK 184

Query: 199 TGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKN 258
           +G+VLIRA C YVLMN+ TRKLSKFC+E++ EMNP FLDS PI+EEDNI+S KL +   +
Sbjct: 185 SGKVLIRAICSYVLMNKRTRKLSKFCEELEEEMNPFFLDSHPILEEDNIVSQKLHLHQHD 244

Query: 259 CNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQ 318
           C   SLVTRLSDLDMN H+NNASYINF+LEGTPR V EN++LS +TIKYLKEC VD+QLQ
Sbjct: 245 CTYASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLSRITIKYLKECSVDQQLQ 304

Query: 319 SLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLWTPK 354
           SLS+P++ND V     + D  ++LHHS+L+D IELVRA T+WTPK
Sbjct: 305 SLSLPLENDVVNVTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 347

BLAST of HG10023318 vs. NCBI nr
Match: XP_022960310.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 437.6 bits (1124), Expect = 1.0e-118
Identity = 222/345 (64.35%), Postives = 259/345 (75.07%), Query Frame = 0

Query: 19  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 78
           SI  +E  FG  +  +L  KG PK     LK+S+I      A     +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPTAGGFESMDDYMSRKPEITR 64

Query: 79  GNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLV----- 138
            NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQESKLNH  SCGLV     
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLG 124

Query: 139 TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYK 198
            TG GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW C HGKNSYRR+W+ R+ K
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKK 184

Query: 199 TGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKN 258
           +G+VLIRA CFYVLMNR TRKLSKFC+E+K EMNP FLDS PI+EEDNI+S KL      
Sbjct: 185 SGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHY 244

Query: 259 CNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQ 318
           C   SLVTRLSDLDMN H+NNASYINFILEGTPR V E ++LS +TIKYLKEC VD+QLQ
Sbjct: 245 CTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQLQ 304

Query: 319 SLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLWTPK 354
           SLS+P++ND V     + D  ++LHHS+L+D IELVRA T+WTPK
Sbjct: 305 SLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 347

BLAST of HG10023318 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.8e-66
Identity = 126/288 (43.75%), Postives = 184/288 (63.89%), Query Frame = 0

Query: 69  YESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLK 128
           ++ R  D+      +G++V DGLV+ ++F ++SYE G D+  ++  ++N LQE+ +NH +
Sbjct: 110 WKPRRPDMVIDPFGIGKIVQDGLVFSQNFSIRSYEIGADQTASIETLMNHLQETAINHCR 169

Query: 129 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWV 188
           S GL+  G G TPEM K+ L+WVV ++QVVVD+YP+W DVVQ+ TW    GKN  RRDW+
Sbjct: 170 SAGLLGEGFGATPEMCKKNLIWVVTRMQVVVDRYPTWGDVVQVDTWVSASGKNGMRRDWL 229

Query: 189 VRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLD 248
           V N +TG++L RAT  +V+MN+ TR+LSK  +E++ E+ P F++SDP++ ED+    KLD
Sbjct: 230 VSNSETGEILTRATSVWVMMNKLTRRLSKIPEEVRGEIEPFFMNSDPVLAEDSQKLVKLD 289

Query: 249 IDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEV 308
                  C  L  + SDLD+N HVNN  YI +ILE  P  + E+H LSA+T++Y +EC  
Sbjct: 290 DSTAEHVCKGLTPKWSDLDVNQHVNNVKYIGWILESAPLPILESHELSALTLEYRRECGR 349

Query: 309 DKQLQSLS--MPIKNDNVLNDSHLQLHHSV-LADEIELVRATTLWTPK 354
           D  LQSL+       +N +N       H + L D  E+VR  T W PK
Sbjct: 350 DSVLQSLTTVSDSNTENAVNVGEFNCQHLLRLDDGAEIVRGRTRWRPK 397

BLAST of HG10023318 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-62
Identity = 121/289 (41.87%), Postives = 189/289 (65.40%), Query Frame = 0

Query: 69  YESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLK 128
           ++ R  D+      +G +V DGLV++++F ++SYE G D++ ++  ++N LQE+ LNH+K
Sbjct: 115 WKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVK 174

Query: 129 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWV 188
           + GL+  G G+TPEM K+ L+WVV ++QVVVD+YP+W DVV++ TW    GKN  RRDW+
Sbjct: 175 TAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWL 234

Query: 189 VRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLD 248
           VR+  TG+ L RA+  +V+MN+ TR+LSK  +E++ E+ P+F++SDP++ ED+   TK+D
Sbjct: 235 VRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAEDSRKLTKID 294

Query: 249 IDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEV 308
               +   + L  R SDLD+N HVNN  YI +ILE  P  + E  +L ++T++Y +EC  
Sbjct: 295 DKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGR 354

Query: 309 DKQLQSLSMPIKND--NVLNDSHLQLHHSV-LADEIELVRATTLWTPKS 355
           D  LQSL+     D  N+     ++  H + L D  E+VR  T W+ K+
Sbjct: 355 DSVLQSLTAVTGCDIGNLATAGDVECQHLLRLQDGAEVVRGRTEWSSKT 403

BLAST of HG10023318 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.5e-62
Identity = 122/283 (43.11%), Postives = 181/283 (63.96%), Query Frame = 0

Query: 72  RSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCG 131
           + LD+      +G +V DGLV++++F ++SYE G D+  ++  ++N LQE+ LNH+K+ G
Sbjct: 114 KRLDMLEDPFGLGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAG 173

Query: 132 LVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRN 191
           L   G G TPEM KR L+WVV K+QV+V++YP+W D V++ TW    GKN  RRDW++ +
Sbjct: 174 LSNDGFGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISD 233

Query: 192 YKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDA 251
             TG++L RA+  +V+MN+ TRKLSK  DE++ E+ PHF+DS P+IE+D+    KLD  +
Sbjct: 234 CNTGEILTRASSVWVMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIEDDDRKLPKLDEKS 293

Query: 252 KNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQ 311
            +     L  R +DLD+N HVNNA YI +ILE TP  V E   L ++T++Y +EC  +  
Sbjct: 294 ADSIRKGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESV 353

Query: 312 LQSLSMPIKNDNVLNDSHLQLHHSVLADEIELVRATTLWTPKS 355
           L+SL+  +        S  Q H   L D  E+V+  T W PK+
Sbjct: 354 LESLT-AVDPSGEGYGSQFQ-HLLRLEDGGEIVKGRTEWRPKN 394

BLAST of HG10023318 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.9e-60
Identity = 117/273 (42.86%), Postives = 174/273 (63.74%), Query Frame = 0

Query: 83  MGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGLGTTPE 142
           +G +V DGLV++++F ++SYE G D+  ++  ++N LQE+ LNH+K  GL   G G TPE
Sbjct: 128 LGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDGFGRTPE 187

Query: 143 MTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYKTGQVLIRAT 202
           M KR L+WVV K+QV+V++YP+W D V++ TW    GKN  RRDW++ +  TG++L RA+
Sbjct: 188 MYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGEILTRAS 247

Query: 203 CFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKNCNCTSLVTR 262
             +V+MN+ TR+LSK  DE++ E+ PHF+DS P+IE+D+    KLD    +     L  R
Sbjct: 248 SVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSPPVIEDDDRKLPKLDEKTADSIRKGLTPR 307

Query: 263 LSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQSLSMPIKND 322
            +DLD+N HVNN  YI +ILE TP  V E   L ++T++Y +EC  +  L+SL+    + 
Sbjct: 308 WNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLTAMDPSG 367

Query: 323 NVLNDSHLQLHHSV-LADEIELVRATTLWTPKS 355
                   Q  H + L D  E+V+  T W PK+
Sbjct: 368 GGYGS---QFQHLLRLEDGGEIVKGRTEWRPKN 397

BLAST of HG10023318 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.6e-53
Identity = 104/268 (38.81%), Postives = 164/268 (61.19%), Query Frame = 0

Query: 87  VNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGLGTTPEMTKR 146
           V DG+ +++ F ++SYE G D+  ++  ++N  QE+ LNH KS GL+  G G TPEM KR
Sbjct: 129 VQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQETSLNHCKSLGLLNDGFGRTPEMCKR 188

Query: 147 GLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYKTGQVLIRATCFYV 206
            L+WVV K+Q+ V++YP+W D +++ TW    GKN   RDW++ +  +G++LIRAT  + 
Sbjct: 189 DLIWVVTKMQIEVNRYPTWGDTIEVTTWVSESGKNGMSRDWLISDCHSGEILIRATSVWA 248

Query: 207 LMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKNCNCTSLVTRLSDL 266
           +MN+ TR+LSK  DE++ E+ P+F+DS P+IE+D  +  KLD+   +     L  R +D 
Sbjct: 249 MMNQKTRRLSKIPDEVRQEIVPYFVDSAPVIEDDRKLH-KLDVKTGDSIRNGLTPRWNDF 308

Query: 267 DMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQSLSMPIKNDNVLN 326
           D+N HVNN  YI ++L+  P  V E   L  +T++Y +EC  D  L+S++    + +   
Sbjct: 309 DVNQHVNNVKYIAWLLKSVPTEVFETQELCGLTLEYRRECRRDSVLESVT--AMDPSKEG 368

Query: 327 DSHLQLHHSVLADEIELVRATTLWTPKS 355
           D  L  H   L +  ++    T W PK+
Sbjct: 369 DRSLYQHLLRLENGADIALGRTEWRPKN 393

BLAST of HG10023318 vs. ExPASy TrEMBL
Match: A0A5A7UZ85 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002510 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.8e-170
Identity = 296/360 (82.22%), Postives = 328/360 (91.11%), Query Frame = 0

Query: 2   MAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 61
           MAA+SSSY CN GKK NS LKKEQRFGD++ GNLQ K HPK+ LE L+TSNIIAKS+IAS
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRTSNIIAKSSIAS 60

Query: 62  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 121
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFLVKSYESGPDK MTMFFIINQL E
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKKMTMFFIINQLHE 120

Query: 122 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKN 181
           SKLNHLKSCGLVTTGLGTTPEMTKRGL+WVVHKLQVVVDQYPSWND+VQL+TW C +GKN
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPEMTKRGLIWVVHKLQVVVDQYPSWNDIVQLQTWSCFYGKN 180

Query: 182 SYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDN 241
           SYRRDW+VRNYKTGQVLIRA+CFYVLMNRNTRKLSKF DEMK EMNPHFL SDPIIEE+N
Sbjct: 181 SYRRDWIVRNYKTGQVLIRASCFYVLMNRNTRKLSKFYDEMKEEMNPHFLKSDPIIEEEN 240

Query: 242 IISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIK 301
           IISTKLD+DAK CNCTSLVTRLSDLDMN+HVNNASYINFILEGTPR + E+HRLS VT+K
Sbjct: 241 IISTKLDVDAKGCNCTSLVTRLSDLDMNHHVNNASYINFILEGTPRWMLESHRLSTVTVK 300

Query: 302 YLKECEVDKQLQSLSMPIKNDNVLN------DSHLQLHHSVLADEIELVRATTLWTPKSI 356
           YLKEC VDK LQSLSMPI+ND + N      +++++LHHS++++ IELVRATTLWTP SI
Sbjct: 301 YLKECGVDKILQSLSMPIQNDVISNGNYLVKENYIKLHHSIVSEGIELVRATTLWTPYSI 360

BLAST of HG10023318 vs. ExPASy TrEMBL
Match: A0A6J1KZF4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497736 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 3.5e-120
Identity = 222/345 (64.35%), Postives = 265/345 (76.81%), Query Frame = 0

Query: 19  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 78
           SI  +E  FG  +  +L  KG PK     LK+S+I     IAS  + +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPIASGFKSMDDYVSRKPEITR 64

Query: 79  GNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGL- 138
            NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQESKLNH  SCGLV TGL 
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVKTGLG 124

Query: 139 ----GTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYK 198
               GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW C HGKNSYRR+W+ R+ K
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICRHGKNSYRRNWIARDKK 184

Query: 199 TGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKN 258
           +G+VLIRA C YVLMN+ TRKLSKFC+E++ EMNP FLDS PI+EEDNI+S KL +   +
Sbjct: 185 SGKVLIRAICSYVLMNKRTRKLSKFCEELEEEMNPFFLDSHPILEEDNIVSQKLHLHQHD 244

Query: 259 CNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQ 318
           C   SLVTRLSDLDMN H+NNASYINF+LEGTPR V EN++LS +TIKYLKEC VD+QLQ
Sbjct: 245 CTYASLVTRLSDLDMNQHINNASYINFVLEGTPRSVLENYQLSRITIKYLKECSVDQQLQ 304

Query: 319 SLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLWTPK 354
           SLS+P++ND V     + D  ++LHHS+L+D IELVRA T+WTPK
Sbjct: 305 SLSLPLENDVVNVTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 347

BLAST of HG10023318 vs. ExPASy TrEMBL
Match: A0A6J1H8Q2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461080 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 5.1e-119
Identity = 222/345 (64.35%), Postives = 259/345 (75.07%), Query Frame = 0

Query: 19  SILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIASEVRKIDSYESRSLDITH 78
           SI  +E  FG  +  +L  KG PK     LK+S+I      A     +D Y SR  +IT 
Sbjct: 5   SISMREPTFGHATTPSLHLKGRPKSASLTLKSSHI--SQPTAGGFESMDDYMSRKPEITR 64

Query: 79  GNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLKSCGLV----- 138
            NL + E +NDGLVYQ HFLV+SYESGPD  MTMFF+INQLQESKLNH  SCGLV     
Sbjct: 65  ENLMVEEFLNDGLVYQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLG 124

Query: 139 TTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWVVRNYK 198
            TG GTT EMTKR L+WVVHKLQVVVDQ+PSWNDVV+LRTW C HGKNSYRR+W+ R+ K
Sbjct: 125 KTGFGTTHEMTKRDLIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKK 184

Query: 199 TGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLDIDAKN 258
           +G+VLIRA CFYVLMNR TRKLSKFC+E+K EMNP FLDS PI+EEDNI+S KL      
Sbjct: 185 SGKVLIRAICFYVLMNRRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHY 244

Query: 259 CNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQ 318
           C   SLVTRLSDLDMN H+NNASYINFILEGTPR V E ++LS +TIKYLKEC VD+QLQ
Sbjct: 245 CTHASLVTRLSDLDMNQHINNASYINFILEGTPRSVLEKYQLSGITIKYLKECSVDQQLQ 304

Query: 319 SLSMPIKNDNV-----LNDSHLQLHHSVLADEIELVRATTLWTPK 354
           SLS+P++ND V     + D  ++LHHS+L+D IELVRA T+WTPK
Sbjct: 305 SLSVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 347

BLAST of HG10023318 vs. ExPASy TrEMBL
Match: A0A0A0K767 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440570 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 4.3e-78
Identity = 146/164 (89.02%), Postives = 156/164 (95.12%), Query Frame = 0

Query: 2   MAAISSSYSCNFGKKMNSILKKEQRFGDTSRGNLQFKGHPKICLERLKTSNIIAKSTIAS 61
           MAAISSSY CNFG++ NS LKKEQRFGD++RGNLQ K HPK CLE L+TSNIIAKS+IAS
Sbjct: 1   MAAISSSYCCNFGEEFNSNLKKEQRFGDSNRGNLQLKNHPKFCLEGLRTSNIIAKSSIAS 60

Query: 62  EVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 121
           EVRKI+SYESR+LDITHGNLTMGEL+NDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE
Sbjct: 61  EVRKINSYESRNLDITHGNLTMGELLNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQE 120

Query: 122 SKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSW 166
           SKLNHLKSCGLVTTGLGTTP MTKRGL+WVVHKLQVVVDQYPSW
Sbjct: 121 SKLNHLKSCGLVTTGLGTTPGMTKRGLIWVVHKLQVVVDQYPSW 164

BLAST of HG10023318 vs. ExPASy TrEMBL
Match: A0A6J1CMR0 (Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC111012889 PE=3 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 5.9e-75
Identity = 142/245 (57.96%), Postives = 185/245 (75.51%), Query Frame = 0

Query: 112 MFFIINQLQESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQL 171
           M  IINQLQES +NH KSCGLV TG  +T EM KRGL+WVVHKLQVVVD YPSWND+VQL
Sbjct: 1   MVSIINQLQESAINHFKSCGLVETGYASTREMDKRGLIWVVHKLQVVVDLYPSWNDIVQL 60

Query: 172 RTWGCVHGKNSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFL 231
            TW CV GKN++RRDW++ + KTG+VLIRA CFY LMN++TRKLS   DE+  ++ P   
Sbjct: 61  STWVCVCGKNNFRRDWIIHDSKTGEVLIRAICFYALMNKDTRKLSNVDDEIMADITPFLS 120

Query: 232 DSDPIIEEDNIISTKLDIDAKNCNCTSLVTRLSDLDMNYHVNN-ASYINFILEGTPRCVS 291
           + DPI++EDN I  KLD+++K+C  + LVT+  DLD+N HV++  SYIN+ILE  P  V 
Sbjct: 121 NCDPIVKEDNSILRKLDVNSKDCIGSHLVTKSCDLDLNQHVSHIISYINWILESAPPMVV 180

Query: 292 ENHRLSAVTIKYLKECEVDKQLQSLSMPIKNDNVLNDSH--LQLHHSVLADEIELVRATT 351
           +NH LSA+TIKY KEC +D++L+SL +PI++DN  ++S   ++  H+   DE ELVRA T
Sbjct: 181 KNHWLSAITIKYRKECGIDRKLKSLYVPIRDDNNGDESEEGIKFQHAFFTDECELVRART 240

Query: 352 LWTPK 354
           +W PK
Sbjct: 241 VWKPK 245

BLAST of HG10023318 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 241.9 bits (616), Expect = 7.8e-64
Identity = 121/289 (41.87%), Postives = 189/289 (65.40%), Query Frame = 0

Query: 69  YESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQESKLNHLK 128
           ++ R  D+      +G +V DGLV++++F ++SYE G D++ ++  ++N LQE+ LNH+K
Sbjct: 115 WKPRRSDMLVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVK 174

Query: 129 SCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGKNSYRRDWV 188
           + GL+  G G+TPEM K+ L+WVV ++QVVVD+YP+W DVV++ TW    GKN  RRDW+
Sbjct: 175 TAGLLGDGFGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWL 234

Query: 189 VRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPIIEEDNIISTKLD 248
           VR+  TG+ L RA+  +V+MN+ TR+LSK  +E++ E+ P+F++SDP++ ED+   TK+D
Sbjct: 235 VRDCNTGETLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAEDSRKLTKID 294

Query: 249 IDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRLSAVTIKYLKECEV 308
               +   + L  R SDLD+N HVNN  YI +ILE  P  + E  +L ++T++Y +EC  
Sbjct: 295 DKTADYVRSGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGR 354

Query: 309 DKQLQSLSMPIKND--NVLNDSHLQLHHSV-LADEIELVRATTLWTPKS 355
           D  LQSL+     D  N+     ++  H + L D  E+VR  T W+ K+
Sbjct: 355 DSVLQSLTAVTGCDIGNLATAGDVECQHLLRLQDGAEVVRGRTEWSSKT 403

BLAST of HG10023318 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 179.5 bits (454), Expect = 4.8e-45
Identity = 110/326 (33.74%), Postives = 171/326 (52.45%), Query Frame = 0

Query: 44  CLERLKTSNIIAKSTIASEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYE 103
           C +  KT  +  ++ ++++   +     + L      L +G L  DGL Y+  F+V+SYE
Sbjct: 39  CSQTKKTGLVPLRAVVSADQGSV----VQGLATLADQLRLGSLTEDGLSYKEKFVVRSYE 98

Query: 104 SGPDKNMTMFFIINQLQESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYP 163
            G +K  T+  I N LQE   NH +S G  T G  TT  M K  L+WV  ++ + + +YP
Sbjct: 99  VGSNKTATVETIANLLQEVGCNHAQSVGFSTDGFATTTTMRKLHLIWVTARMHIEIYKYP 158

Query: 164 SWNDVVQLRTWGCVHGKNSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMK 223
           +W DVV++ TW    G+   RRDW++++  TG+V  RAT  +V+MN++TR+L K  D+++
Sbjct: 159 AWGDVVEIETWCQSEGRIGTRRDWILKDSVTGEVTGRATSKWVMMNQDTRRLQKVSDDVR 218

Query: 224 VEMNPHFLDSDPII---EEDNIISTKLD--IDAKNCNCTSLVTRLSDLDMNYHVNNASYI 283
            E    F   +P +   EE+N    K+    D    +   L  R +DLDMN HVNN +YI
Sbjct: 219 DEYLV-FCPQEPRLAFPEENNRSLKKIPKLEDPAQYSMIGLKPRRADLDMNQHVNNVTYI 278

Query: 284 NFILEGTPRCVSENHRLSAVTIKYLKECEVDKQLQSL---SMPIKNDNVLNDSHLQLHHS 343
            ++LE  P+ + + H L  +T+ Y +EC+ D  + SL   +  I   N    S  Q H+ 
Sbjct: 279 GWVLESIPQEIVDTHELQVITLDYRRECQQDDVVDSLTTTTSEIGGTNGSATSGTQGHND 338

Query: 344 --------VLADEIELVRATTLWTPK 354
                   +  D  E+ R TTLW  K
Sbjct: 339 SQFLHLLRLSGDGQEINRGTTLWRKK 359

BLAST of HG10023318 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 173.3 bits (438), Expect = 3.4e-43
Identity = 96/261 (36.78%), Postives = 149/261 (57.09%), Query Frame = 0

Query: 61  SEVRKIDSYESRSLDITHGNLTMGELVNDGLVYQRHFLVKSYESGPDKNMTMFFIINQLQ 120
           S VR  D+    S       L  G L+ DG  Y+  F+V+SYE G +K  T+  I N LQ
Sbjct: 54  SSVRVADTVSGTS---PADRLRFGRLMEDGFSYKEKFIVRSYEVGINKTATIETIANLLQ 113

Query: 121 ESKLNHLKSCGLVTTGLGTTPEMTKRGLLWVVHKLQVVVDQYPSWNDVVQLRTWGCVHGK 180
           E   NH+++ G  T G  TT  M K  L+WV  ++ + + +YP+W+DVV++ TW    G+
Sbjct: 114 EVACNHVQNVGFSTDGFATTLTMRKLHLIWVTARMHIEIYKYPAWSDVVEIETWCQSEGR 173

Query: 181 NSYRRDWVVRNYKTGQVLIRATCFYVLMNRNTRKLSKFCDEMKVEMNPHFLDSDPII--- 240
              RRDW++++  TG+V+ RAT  +V+MN++TR+L +  DE++ E    F   +P +   
Sbjct: 174 IGTRRDWILKDCATGEVIGRATSKWVMMNQDTRRLQRVTDEVRDEYLV-FCPPEPRLAFP 233

Query: 241 EEDNIISTKLD--IDAKNCNCTSLVTRLSDLDMNYHVNNASYINFILEGTPRCVSENHRL 300
           EE+N    K+    D    +   L  R +DLDMN HVNN +YI ++LE  P+ + + H L
Sbjct: 234 EENNSSLKKIPKLEDPAQYSMLGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIIDTHEL 293

Query: 301 SAVTIKYLKECEVDKQLQSLS 317
             +T+ Y +EC+ D  + SL+
Sbjct: 294 KVITLDYRRECQQDDIVDSLT 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0058801.15.8e-17082.22palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596... [more]
KAG6593401.13.1e-12363.36Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
XP_023514527.15.4e-12363.26palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo ... [more]
XP_023004418.17.2e-12064.35palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita maxim... [more]
XP_022960310.11.0e-11864.35palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
Q9SQI32.8e-6643.75Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum... [more]
Q9SJE21.1e-6241.87Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia... [more]
G3ESV05.5e-6243.11Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ... [more]
Q395133.9e-6042.86Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ... [more]
G3ESV13.6e-5338.81Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim... [more]
Match NameE-valueIdentityDescription
A0A5A7UZ852.8e-17082.22Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1KZF43.5e-12064.35Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149773... [more]
A0A6J1H8Q25.1e-11964.35Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461... [more]
A0A0A0K7674.3e-7889.02Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440570... [more]
A0A6J1CMR05.9e-7557.96Acyl-[acyl-carrier-protein] hydrolase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
AT1G08510.17.8e-6441.87fatty acyl-ACP thioesterases B [more]
AT3G25110.14.8e-4533.74fatA acyl-ACP thioesterase [more]
AT4G13050.13.4e-4336.78Acyl-ACP thioesterase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002864Acyl-ACP thioesterasePFAMPF01643Acyl-ACP_TEcoord: 90..351
e-value: 6.9E-57
score: 192.6
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 83..355
e-value: 7.6E-72
score: 243.6
NoneNo IPR availablePANTHERPTHR31727:SF2PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTICcoord: 53..354
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 53..354
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 88..239
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 250..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023318.1HG10023318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016297 acyl-[acyl-carrier-protein] hydrolase activity
molecular_function GO:0016790 thiolester hydrolase activity