HG10023309 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10023309
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr05: 32888962 .. 32890200 (+)
RNA-Seq Expression	HG10023309
Synteny	HG10023309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGGCAGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGTAATTAACCCCACAATCCCTTTTTTCTTTTTCATAACTAAATATTTACTCTCCCAACTTTTATCACCTAATCGAGATGGTAAAATAAATAAATTACAAATTTACTTCATCAACTTTAAGACATTTCTCGCTATTTTGAAGTTTGAAGTTTATTTCAAAACAATTATTGTAAGCTTTATTTTACCATTAAGTTCATAGACAAATCATACAGATAAGGCAAATAAAGACACATGACACATTCGTGAAATATATATTTGTTGTCGTCTTCCCTCCTTTCAACCTCACATGGGTGTTTATGATATTTTTTTTTTTTTTTTTGTTAGCCCTAACTCCCAAATTTACTTCTCATGTGTTTTTAGATTTGTTTACTTAAAACAACACATAAGCAAACACAAACTTTTTCATCCCATCCATGAATTTAACAAGAAAAACCTTCCACGGTTATGTTTTAAGGGAAAAATAACTTCAAACTTCAAGGAAGAGTAATAATTGAAAACCAACCACATACTTCAAGGGTACTTTTGATAATTCAACATTTTTAAAATTCACGAATCTAATAGATACAAGATTAATAAAAATCTACTTGTAAATGTAAACTTCATAATATAAAGACTAAACTTATAATTTAAGCGTATGTTATGTTATGGATTGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

mRNA sequence

ATGATTGGCAGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

Coding sequence (CDS)

ATGATTGGCAGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

Protein sequence

MIGSETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNVSRIKDYCGI

Homology

[Close]

BLAST of HG10023309 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 350.5 bits (898), Expect = 9.1e-93
Identity = 165/188 (87.77%), Postives = 179/188 (95.21%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TVERFSTLVA+LEKMVTKMV+ESYG+SK+V+E NMES +YL RC+KYR PEKDE+DVGLH
Sbjct: 129 TVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEKDETDVGLH 188

Query: 66  SHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRH 125
           SHTDLTFFSIIHQHLISGLQ+QSLDDQWIDI PS HC F+VMAGDALMAWSNERIRSCRH
Sbjct: 189 SHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSNERIRSCRH 248

Query: 126 QVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 185
           +VIM+GEETRYSIG+FSFK+ TIAVPEE VDDANPLRYKPFNHYDFLTYDKAKASHQNVS
Sbjct: 249 RVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 308

Query: 186 RIKDYCGI 194
           RIKDYCGI
Sbjct: 309 RIKDYCGI 316

[Close]

BLAST of HG10023309 vs. NCBI nr
Match: KAA0058792.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa] >TYK10586.1 putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 337.8 bits (865), Expect = 6.1e-89
Identity = 161/189 (85.19%), Postives = 177/189 (93.65%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TVERFSTLVAKLEKMVTKMV+ESYGVSKVV+E  MEST+YL R +KYRV EKDE+DVGLH
Sbjct: 55  TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 114

Query: 66  SHTDLTFFSIIHQHLISGLQIQSL-DDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 125
           SHTDLTFFSIIHQHLISGLQIQSL DDQW+D+HPS+HCSF+VMAGDALMAWSNERIRSCR
Sbjct: 115 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 174

Query: 126 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 185
           H+VIM+GEETRYSIG+FSFK+ TI VP+ FVDDANPLRYKPFNHYDFLTYDKA ASH  +
Sbjct: 175 HKVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 234

Query: 186 SRIKDYCGI 194
           SRIKDYCG+
Sbjct: 235 SRIKDYCGL 243

[Close]

BLAST of HG10023309 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 337.8 bits (865), Expect = 6.1e-89
Identity = 161/189 (85.19%), Postives = 177/189 (93.65%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TVERFSTLVAKLEKMVTKMV+ESYGVSKVV+E  MEST+YL R +KYRV EKDE+DVGLH
Sbjct: 128 TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 187

Query: 66  SHTDLTFFSIIHQHLISGLQIQSL-DDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 125
           SHTDLTFFSIIHQHLISGLQIQSL DDQW+D+HPS+HCSF+VMAGDALMAWSNERIRSCR
Sbjct: 188 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 247

Query: 126 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 185
           H+VIM+GEETRYSIG+FSFK+ TI VP+ FVDDANPLRYKPFNHYDFLTYDKA ASH  +
Sbjct: 248 HRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 307

Query: 186 SRIKDYCGI 194
           SRIKDYCG+
Sbjct: 308 SRIKDYCGL 316

[Close]

BLAST of HG10023309 vs. NCBI nr
Match: XP_004136073.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 156/188 (82.98%), Postives = 173/188 (92.02%), Query Frame = 0


Query: 7   VERFSTLVAKLEKMVTKMVMESYGVS-KVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 66
           VERFSTLVAKLE MVTKMV+ESYGVS KVVSE  MEST+YL R +KYRV EKDE+DVGLH
Sbjct: 129 VERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDENDVGLH 188

Query: 67  SHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRH 126
           SHTDLTF SIIHQHLISGLQIQ LDDQW+++HPS+HCSF VMAGDALMAWSNERIRSCRH
Sbjct: 189 SHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNERIRSCRH 248

Query: 127 QVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 186
           +VIM+GEETRYSIG+FSFK+  I VP+EFVDDANPLRYKPF+HYDFLTYDKA ASH+ +S
Sbjct: 249 RVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANASHKTIS 308

Query: 187 RIKDYCGI 194
           RIKDYCG+
Sbjct: 309 RIKDYCGL 316

[Close]

BLAST of HG10023309 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 309.7 bits (792), Expect = 1.8e-80
Identity = 144/190 (75.79%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVG 63
           SET++RFSTLVAKL+K+VT+MV+ESYGVSK + ++N++ST  L R +KYRVPE+ E+DVG
Sbjct: 127 SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQHETDVG 186

Query: 64  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 123
           LH+HTDLTFFSIIHQHLI GLQIQSLDDQWID+ PS+HCSF+VMAGDALMAWSN+RIRSC
Sbjct: 187 LHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSC 246

Query: 124 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 183
           RH+V MK EETRYS G+FSFK+ TI+VPEE VDDANPLRYKPFNHYDFLT+DKAK S + 
Sbjct: 247 RHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKAKGSKKF 306

Query: 184 VSRIKDYCGI 194
           + RI+DYC I
Sbjct: 307 LYRIRDYCSI 316

[Close]

BLAST of HG10023309 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.4e-34
Identity = 78/182 (42.86%), Postives = 115/182 (63.19%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD-- 63
           SET+  FS  + +L+ MV +M+MES+G+ K + E ++ ST YL R  KY  P  D+ D  
Sbjct: 120 SETIHLFSEQLVELDLMVRRMIMESFGIEKYIDE-HLNSTYYLTRLMKYTSPPDDDDDEE 179

Query: 64  --VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNER 123
             +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N R
Sbjct: 180 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 239

Query: 124 IRSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDKA 180
           + S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + + 
Sbjct: 240 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 299

[Close]

BLAST of HG10023309 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.7e-33
Identity = 77/183 (42.08%), Postives = 114/183 (62.30%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD-- 63
           SET+  FS  + +L+ MV +M+MES+G+   + E ++ ST YL R  KY  P  D+ D  
Sbjct: 127 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDE 186

Query: 64  ---VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNE 123
              +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N 
Sbjct: 187 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNG 246

Query: 124 RIRSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDK 180
           R+ S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + +
Sbjct: 247 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 306

[Close]

BLAST of HG10023309 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.2e-33
Identity = 77/184 (41.85%), Postives = 114/184 (61.96%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD-- 63
           SET+  FS  + +L+ MV +M+MES+G+   + E ++ ST YL R  KY  P  D+ D  
Sbjct: 127 SETIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDD 186

Query: 64  ----VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSN 123
               +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N
Sbjct: 187 EETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLN 246

Query: 124 ERIRSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYD 180
            R+ S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + 
Sbjct: 247 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 306

[Close]

BLAST of HG10023309 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.1e-24
Identity = 63/186 (33.87%), Postives = 99/186 (53.23%), Query Frame = 0


Query: 5   ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 64
           ETV  +   +A+++ ++  MV ES+G+ K   +S  EST+YL    +Y+ P KD     L
Sbjct: 123 ETVYSYGKRMAEVDHILKMMVFESFGMEKHF-DSFCESTNYLLHFMRYQQPGKDGRSPAL 182

Query: 65  HSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 124
             H D +  +I++Q+ + GL+ ++ D +WI      H   +V+ GD  MAWSN R+ S  
Sbjct: 183 SLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNGRLHSPL 242

Query: 125 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 184
           H+V +   + R S   FSF  + I  P E VD+ +PL + PF   + L Y   K   + V
Sbjct: 243 HRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAV 302

Query: 185 SRIKDY 191
             +K Y
Sbjct: 303 CDLKQY 304

[Close]

BLAST of HG10023309 vs. ExPASy Swiss-Prot
Match: Q945B4 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 50/138 (36.23%), Postives = 87/138 (63.04%), Query Frame = 0


Query: 59  ESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNE 118
           ++++GL SHTD +   II+QH I GL++++ + +WI + P+ + + +V+AGDAL A  N 
Sbjct: 270 DAELGLPSHTDKSLSGIIYQHQIDGLEVKTKEGKWIRVKPAPN-TVIVIAGDALCALMNG 329

Query: 119 RIRSCRHQV-IMKGEETRYSIGLFSFKSE--TIAVPEEFVDDANPLRYKPFNHYDFLTYD 178
           RI S  H+V + + ++TRY+  LFS+  E   I  P+E VD+ +P  +KPF+  D   + 
Sbjct: 330 RIPSPYHRVRVTERKKTRYAAALFSYPKEGYIIDSPKELVDEKHPRAFKPFDFVDLFNFY 389

Query: 179 KAKASHQNVSRIKDYCGI 194
             +A  +  S ++ +CG+
Sbjct: 390 HTEAGRRAPSTLQAFCGV 406

[Close]

BLAST of HG10023309 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 161/189 (85.19%), Postives = 177/189 (93.65%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TVERFSTLVAKLEKMVTKMV+ESYGVSKVV+E  MEST+YL R +KYRV EKDE+DVGLH
Sbjct: 55  TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 114

Query: 66  SHTDLTFFSIIHQHLISGLQIQSL-DDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 125
           SHTDLTFFSIIHQHLISGLQIQSL DDQW+D+HPS+HCSF+VMAGDALMAWSNERIRSCR
Sbjct: 115 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 174

Query: 126 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 185
           H+VIM+GEETRYSIG+FSFK+ TI VP+ FVDDANPLRYKPFNHYDFLTYDKA ASH  +
Sbjct: 175 HKVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 234

Query: 186 SRIKDYCGI 194
           SRIKDYCG+
Sbjct: 235 SRIKDYCGL 243

[Close]

BLAST of HG10023309 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.0e-89
Identity = 161/189 (85.19%), Postives = 177/189 (93.65%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TVERFSTLVAKLEKMVTKMV+ESYGVSKVV+E  MEST+YL R +KYRV EKDE+DVGLH
Sbjct: 128 TVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLH 187

Query: 66  SHTDLTFFSIIHQHLISGLQIQSL-DDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 125
           SHTDLTFFSIIHQHLISGLQIQSL DDQW+D+HPS+HCSF+VMAGDALMAWSNERIRSCR
Sbjct: 188 SHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCR 247

Query: 126 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 185
           H+VIM+GEETRYSIG+FSFK+ TI VP+ FVDDANPLRYKPFNHYDFLTYDKA ASH  +
Sbjct: 248 HRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTI 307

Query: 186 SRIKDYCGI 194
           SRIKDYCG+
Sbjct: 308 SRIKDYCGL 316

[Close]

BLAST of HG10023309 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.7e-81
Identity = 144/190 (75.79%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVG 63
           SET++RFSTLVAKL+K+VT+MV+ESYGVSK + ++N++ST  L R +KYRVPE+ E+DVG
Sbjct: 127 SETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQHETDVG 186

Query: 64  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 123
           LH+HTDLTFFSIIHQHLI GLQIQSLDDQWID+ PS+HCSF+VMAGDALMAWSN+RIRSC
Sbjct: 187 LHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSNDRIRSC 246

Query: 124 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 183
           RH+V MK EETRYS G+FSFK+ TI+VPEE VDDANPLRYKPFNHYDFLT+DKAK S + 
Sbjct: 247 RHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKAKGSKKF 306

Query: 184 VSRIKDYCGI 194
           + RI+DYC I
Sbjct: 307 LYRIRDYCSI 316

[Close]

BLAST of HG10023309 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.3e-80
Identity = 142/190 (74.74%), Postives = 170/190 (89.47%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVG 63
           SET++RFSTL+AKL+K++T+MV+ESYGV+K + ++N++ST  L R +KYRVPE+ E+DVG
Sbjct: 127 SETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHETDVG 186

Query: 64  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 123
           LH+HTDLTFFSIIHQHLI GLQIQSLDDQWID+ PS HCSFVVMAGDALMAWSN+RIRSC
Sbjct: 187 LHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDRIRSC 246

Query: 124 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 183
           RH+V MK EETRYS G+FSFK+ TI+VPEE VDDANPLRYKPFNH+DFLTYDKAK S + 
Sbjct: 247 RHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAKGSKKF 306

Query: 184 VSRIKDYCGI 194
           + RI+DYC I
Sbjct: 307 LYRIRDYCSI 316

[Close]

BLAST of HG10023309 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.2e-74
Identity = 136/193 (70.47%), Postives = 167/193 (86.53%), Query Frame = 0


Query: 5   ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 64
           ETV  FSTLVAKLE+ VT+MV+ESYGVSK V ES++EST +L RC+KYRVP++DE+D+GL
Sbjct: 126 ETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDETDLGL 185

Query: 65  HSHTDLTFFSIIHQHLISGLQIQSLDD----QWIDIHPSTHCSFVVMAGDALMAWSNERI 124
           HSHTDLTF SI+HQHLI GLQ++SLD+    +WIDI P    SF+VMAGDALMAWSN+RI
Sbjct: 186 HSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMAWSNDRI 245

Query: 125 RSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKAS 184
           R CRH+V+M+G+ETRYS+GLFSFKS TI+VPEE V+D +PL+YKPF+H++FL YDKA AS
Sbjct: 246 RGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYDKANAS 305

Query: 185 HQNVSRIKDYCGI 194
           H+  SRIKDYCGI
Sbjct: 306 HKTCSRIKDYCGI 318

[Close]

BLAST of HG10023309 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 3.8e-44
Identity = 85/188 (45.21%), Postives = 125/188 (66.49%), Query Frame = 0


Query: 6   TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 65
           TV  FS  VA+L+++V +M+ E+YGV K   ES++ S  YL +  KY  P +  S     
Sbjct: 129 TVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISMPAFP 188

Query: 66  SHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRH 125
            HTD TF SI+HQ+ ++GL+++S D +WI +      S+VVMAGD  M WSN+RIRSC H
Sbjct: 189 QHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCEH 248

Query: 126 QVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNVS 185
           +V M+G++TRY++GLFSF ++ +++PEE VDD +PL YKPF++   + +   K   +  S
Sbjct: 249 RVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANS 308

Query: 186 RIKDYCGI 194
            +K YCGI
Sbjct: 309 TLKAYCGI 314

[Close]

BLAST of HG10023309 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 4.6e-42
Identity = 80/189 (42.33%), Postives = 127/189 (67.20%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVG 63
           SE + +++   A+L++MVT+MV +SY V K   +  +EST YL R  K R P  +   +G
Sbjct: 122 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLG 181

Query: 64  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 123
             +HTD +F +I+HQ  ++GL++++ + + I+I+ S+   F+V+AGDALMAWSN+R+ S 
Sbjct: 182 FVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSP 241

Query: 124 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 183
           RHQV++ GE  RYS+G+F+F + T+ VPEE +D  +PL YKPF+H   L + +    +++
Sbjct: 242 RHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKS 301

Query: 184 VSRIKDYCG 193
              IK YCG
Sbjct: 302 ECPIKAYCG 309

[Close]

BLAST of HG10023309 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.5e-40
Identity = 83/195 (42.56%), Postives = 124/195 (63.59%), Query Frame = 0


Query: 5   ETVERFSTLVAKLEKMVTKMVMESYGV-SKVVSESNMESTDYLFRCYKYRVPEKDESDVG 64
           +T   ++  +A+L++ V +M+ ESYG+  K  S S+ EST YL R   YR  +  E++ G
Sbjct: 127 QTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTG 186

Query: 65  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 124
             SHTD +F SI+HQ+ + GLQ++++  QW+  +PS    FVV++G  L AWSN+RI++C
Sbjct: 187 FVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSP-TRFVVLSGMGLTAWSNDRIKAC 246

Query: 125 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTY-----DKAK 184
            H+V+M  +E RYS+G FSF   TI  PEE VDD +PLRY PF H   L +     +  K
Sbjct: 247 YHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLK 306

Query: 185 ASHQNVSRIKDYCGI 194
            S +++ +I  YCG+
Sbjct: 307 KSSEDLLQI--YCGV 318

[Close]

BLAST of HG10023309 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 4.3e-40
Identity = 79/192 (41.15%), Postives = 127/192 (66.15%), Query Frame = 0


Query: 4   SETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVG 63
           S T++ FS  +++L+  + +M+MES+G+ K + E ++ ST+YL R  KY+ P+ +E+ VG
Sbjct: 128 STTIQSFSKKLSELDITIRRMIMESFGLDKYIDE-HLHSTNYLLRVMKYKGPDTEETKVG 187

Query: 64  LHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 123
           L++HTD    +I++Q+ + GL++Q+ D  WI + P T  SF VM GD+L A  N R+ S 
Sbjct: 188 LNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-TQDSFTVMIGDSLYALLNGRLHSP 247

Query: 124 RHQVIMKGEETRYSIGLFSF--KSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASH 183
            H+V+M G ETRYS+GLFS       ++ P+E VD+ +P  +KPF+H +FL +   +A  
Sbjct: 248 YHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 307

Query: 184 QNVSRIKDYCGI 194
           ++ S +K YCGI
Sbjct: 308 RSQSALKTYCGI 317

[Close]

BLAST of HG10023309 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 9.0e-38
Identity = 86/192 (44.79%), Postives = 117/192 (60.94%), Query Frame = 0


Query: 5   ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 64
           ETV  ++ + A+LE++V +M+ ESY V K  +E  +  T YL R  KYR     E +   
Sbjct: 130 ETVHAYAKMQAELEQLVIRMLFESYNVEK-YTEKYIGGTRYLLRLLKYRRLPNGEPNRKF 189

Query: 65  HSHTDLTFFSIIHQHLISGLQIQS-LDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSC 124
            SHTD +F SI+HQ+ I+GL ++S  +D W    PS    FVV+AGDA+MAWSN+RI++C
Sbjct: 190 ISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSP-TRFVVIAGDAIMAWSNDRIKAC 249

Query: 125 RHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 184
            H+V M+  E RYS+G FSF+   I+ PEE VD  +PL Y PF+H   L Y +    H  
Sbjct: 250 YHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVHLK 309

Query: 185 VSR--IKDYCGI 194
             R   K YCGI
Sbjct: 310 AHRTMTKAYCGI 319

The following BLAST results are available for this feature:

BLAST of HG10023309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898684.1	9.1e-93	87.77	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
KAA0058792.1	6.1e-89	85.19	putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo var....	[more]
XP_008461057.1	6.1e-89	85.19	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi...	[more]
XP_004136073.1	1.4e-85	82.98	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus]	[more]
XP_022953943.1	1.8e-80	75.79	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]

BLAST of HG10023309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q944Z5	3.4e-34	42.86	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q945B6	1.7e-33	42.08	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	2.2e-33	41.85	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
A0A0N9HQ36	1.1e-24	33.87	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B4	2.8e-20	36.23	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of HG10023309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7USF0	3.0e-89	85.19	Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va...	[more]
A0A1S3CDV2	3.0e-89	85.19	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX...	[more]
A0A6J1GPR2	8.7e-81	75.79	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1JXN6	7.3e-80	74.74	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1CXE5	1.2e-74	70.47	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]

BLAST of HG10023309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	3.8e-44	45.21	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	4.6e-42	42.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	2.5e-40	42.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	4.3e-40	41.15	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	9.0e-38	44.79	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 49..143 e-value: 4.3E-16 score: 59.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..174 e-value: 1.3E-37 score: 132.1
None	No IPR available	PANTHER	PTHR47990:SF169	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE	coord: 5..192
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 5..192
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 5..174
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 42..146 score: 9.819657

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10023309.1	HG10023309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding