HG10023308 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023308
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description2-oxoglutarate-dependent dioxygenase AOP1
LocationChr05: 32887397 .. 32887774 (+)
RNA-Seq ExpressionHG10023308
SyntenyHG10023308
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGGTAA

mRNA sequence

ATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGGTAA

Coding sequence (CDS)

ATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGGTAA

Protein sequence

MVSEAKGKIPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFW
Homology
BLAST of HG10023308 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 226.1 bits (575), Expect = 1.7e-55
Identity = 105/123 (85.37%), Postives = 113/123 (91.87%), Query Frame = 0

Query: 3   SEAKGKI-PSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEAT 62
           SEAK K+ P+IDLSK+E GG EEWGLACKEMR+GLEEFGCFSVVYD VSLELHNSIFEAT
Sbjct: 4   SEAKAKVLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIFEAT 63

Query: 63  KQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATEN 122
           K+LFHLP+ETR+KNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQA  +FANLMWP  EN
Sbjct: 64  KELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPTKEN 123

Query: 123 NRF 125
           N F
Sbjct: 124 NHF 126

BLAST of HG10023308 vs. NCBI nr
Match: XP_031744410.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis sativus] >KAE8646456.1 hypothetical protein Csa_016394 [Cucumis sativus])

HSP 1 Score: 213.4 bits (542), Expect = 1.1e-51
Identity = 103/126 (81.75%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G K EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTK-EWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRF 125
           TENN F
Sbjct: 121 TENNHF 125

BLAST of HG10023308 vs. NCBI nr
Match: XP_004136073.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 213.4 bits (542), Expect = 1.1e-51
Identity = 103/126 (81.75%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G K EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTK-EWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRF 125
           TENN F
Sbjct: 121 TENNHF 125

BLAST of HG10023308 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 209.5 bits (532), Expect = 1.6e-50
Identity = 101/126 (80.16%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP+IDLSK+E G K EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI 
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTK-EWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIV 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+L++  +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPT 120

Query: 121 TENNRF 125
            +NN F
Sbjct: 121 RDNNHF 125

BLAST of HG10023308 vs. NCBI nr
Match: XP_038899274.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida])

HSP 1 Score: 201.8 bits (512), Expect = 3.4e-48
Identity = 96/124 (77.42%), Postives = 106/124 (85.48%), Query Frame = 0

Query: 3   SEAKGKI-PSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEAT 62
           SEAK K+ P IDLSK+E  G EEWG ACKEMR+GLEEFGCFSVVYD+VSLELHNSIFEAT
Sbjct: 6   SEAKAKVLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 65

Query: 63  KQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIH-NFANLMWPATE 122
           K+LFHLP+ETR KNTSEKPYHGYFGGYS+LPLYESM IDNP ELQ    + ANL+WP+  
Sbjct: 66  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 125

Query: 123 NNRF 125
           NN F
Sbjct: 126 NNHF 129

BLAST of HG10023308 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-17
Identity = 43/111 (38.74%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWP 118
           LPI T+ +N S KP+HGY        LYES+GID+   L+ +++F   +WP
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWP 119

BLAST of HG10023308 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 2.5e-17
Identity = 46/116 (39.66%), Postives = 67/116 (57.76%), Query Frame = 0

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+L+ K    G  +W     ++R  LE+FGCF   YD+VSLEL  SI +  ++LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENN 123
           LP+ET+ +N   KPY GY    +   L ES+GI N   L+ I+ F   +WP  + N
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGN 119

BLAST of HG10023308 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.2e-17
Identity = 42/111 (37.84%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWP 118
           LPI T+ +N S KP+HGY        LYES+GI++   L+ +++F   +WP
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWP 119

BLAST of HG10023308 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.2e-17
Identity = 45/116 (38.79%), Postives = 67/116 (57.76%), Query Frame = 0

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+L+ K    G  +W     ++R  LE+FGCF   YD+VSLEL  SI +  ++LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENN 123
           LP+ET+ +N   KPY GY    +   L ES+GI N   L+ ++ F   +WP  + N
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGN 119

BLAST of HG10023308 vs. ExPASy Swiss-Prot
Match: Q944Z9 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.5e-17
Identity = 45/116 (38.79%), Postives = 67/116 (57.76%), Query Frame = 0

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+L+ K    G  +W     ++R  L++FGCF   YD+VSLEL  SI +  ++LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALQDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENN 123
           LP+ET+ +N   KPY GY    +   L ES+GI N   L+ I+ F   +WP  + N
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGN 119

BLAST of HG10023308 vs. ExPASy TrEMBL
Match: A0A0A0K8Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.5e-52
Identity = 103/126 (81.75%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G K EWGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTK-EWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRF 125
           TENN F
Sbjct: 121 TENNHF 125

BLAST of HG10023308 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.9e-51
Identity = 101/126 (80.16%), Postives = 110/126 (87.30%), Query Frame = 0

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP+IDLSK+E G K EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI 
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTK-EWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIV 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+L++  +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPT 120

Query: 121 TENNRF 125
            +NN F
Sbjct: 121 RDNNHF 125

BLAST of HG10023308 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.5e-41
Identity = 85/119 (71.43%), Postives = 99/119 (83.19%), Query Frame = 0

Query: 1   MVSEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFE 60
           M ++AK KIP+I+LS+ +   G EEW L  KE+R+ LEEFGCF VVY++VSLELHNSIFE
Sbjct: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60

Query: 61  ATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 119
           ATK+LFHLPIETR++NTSEKPYHGYFG YS LPLYESM +DNP  LQAI  F NLMWPA
Sbjct: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 119

BLAST of HG10023308 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.9e-41
Identity = 84/126 (66.67%), Postives = 96/126 (76.19%), Query Frame = 0

Query: 1   MVSEAKGKIPSIDLSKIE--GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           M SE + ++P IDLSK+E    G E WG  CK MR+GLEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
            A  +LF LPIETR+KNTSEK YHGYFG YS LPLYESM I+NP +L A  +FANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 TENNRF 125
             NN F
Sbjct: 121 QGNNHF 126

BLAST of HG10023308 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.7e-41
Identity = 84/126 (66.67%), Postives = 95/126 (75.40%), Query Frame = 0

Query: 1   MVSEAKGKIPSIDLSKIE--GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           M SE +  +P IDLSK+E    G + WG  CK MR+GLEEFGCF VV++EVSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
            A  +LF LPIETR+KNTSEK YHGYFG YS LPLYESM IDNP +L A  +FANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 TENNRF 125
             NNRF
Sbjct: 121 QGNNRF 126

BLAST of HG10023308 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 6.9e-23
Identity = 51/119 (42.86%), Postives = 71/119 (59.66%), Query Frame = 0

Query: 8   KIPSIDLSKIE--GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLF 67
           K+P +DL+  +        W    +E    LEE+GCF  VYD V+ +L +SIF A ++LF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  HLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRF 125
            LP ET+ KN +EKPYHGY G   V+PL+E +G+D     +    F +LMWP   N+RF
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRF 126

BLAST of HG10023308 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 5.8e-22
Identity = 44/111 (39.64%), Postives = 71/111 (63.96%), Query Frame = 0

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S K    G+ EW L   +++  L+++G F   +D +  EL  S+F A ++LF 
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWP 118
           LP++T+++N S+KP+HGY G Y ++PLYESMGID+    + +  F   +WP
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP 121

BLAST of HG10023308 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 1.9e-20
Identity = 47/112 (41.96%), Postives = 70/112 (62.50%), Query Frame = 0

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSV-VYDEVSLELHNSIFEATKQLF 67
           KIP++D S+ +   G + W    + +R  LEE+GCF + + D+  L+L + +F +   LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 68  HLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWP 118
            LP +T+MKN  +KP +GY G    LPL+ES+GIDN   L+A  +F  LMWP
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP 115

BLAST of HG10023308 vs. TAIR 10
Match: AT1G52810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 9.3e-20
Identity = 42/116 (36.21%), Postives = 70/116 (60.34%), Query Frame = 0

Query: 8   KIPSIDL-SKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID  S+    G  +W     ++R  LEE+GCF  ++D+V LEL  ++F+A++++F 
Sbjct: 11  QLPVIDFTSRDLKPGTIQWDSVRADVRRALEEYGCFEALFDKVPLELRKAVFDASEEVFQ 70

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENN 123
           LP+ET+ +  S++ Y GY G    LPL+E MG+D       ++ F + +WP   NN
Sbjct: 71  LPLETKKRVVSKRKYRGYVGQIPTLPLFEVMGVDFAENPDKVNAFTHKLWPQGNNN 126

BLAST of HG10023308 vs. TAIR 10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 2.3e-18
Identity = 42/111 (37.84%), Postives = 67/111 (60.36%), Query Frame = 0

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWP 118
           LPI T+ +N S KP+HGY        LYES+GI++   L+ +++F   +WP
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWP 119

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898684.11.7e-5585.37probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida][more]
XP_031744410.11.1e-5181.75probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis ... [more]
XP_004136073.11.1e-5181.75probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus][more]
XP_008461057.11.6e-5080.16PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
XP_038899274.13.4e-4877.42probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q945B61.1e-1738.74Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
Q9ZTA22.5e-1739.66Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
Q9ZTA33.2e-1737.84Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
Q945B53.2e-1738.792-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Q944Z95.5e-1738.792-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
A0A0A0K8Y25.5e-5281.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1[more]
A0A1S3CDV27.9e-5180.16probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
A0A6J1CXE51.5e-4171.43probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367... [more]
A0A6J1JXN61.9e-4166.67probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1GPR25.7e-4166.67probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT1G52800.16.9e-2342.862-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52820.15.8e-2239.642-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52790.11.9e-2041.962-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G52810.19.3e-2036.212-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G03070.12.3e-1837.842-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..86
e-value: 1.9E-12
score: 48.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..122
e-value: 1.4E-15
score: 59.3
NoneNo IPR availablePANTHERPTHR47990:SF1692-OXOGLUTARATE-DEPENDENT DIOXYGENASEcoord: 6..122
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..122
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023308.1HG10023308.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding