HG10023307 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10023307
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr05: 32881026 .. 32884126 (+)
RNA-Seq Expression	HG10023307
Synteny	HG10023307

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTAATTCTGAAGCAAAAGCCAAAGTCCCCATAATAGATTTGTCAAAATTAGAGGCAGCTGGCACAGAAGAATGGGGCTTAACATGCAAAGAGATGAGGCTTGGTTTAGAAGAATTTGGTTGCTTTACAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCACAACTCAATTTTTGAAGAAACAAAAGAACTTTTTCATCTCCCAATTGAAACTAGAATAAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGACAATCCAACTCACCTACAACCAACTCACAGTTTTGCTAATCTCATGTGGCCTGCTCGTCCAAACAATCACTTCTGGTAATTTAATTAATTTATTTCAATCCTTCTTATAGTTTTAAAAGTTTTGATTCATCATTCCATACTAATTTAGTTTCAATTTGATTCTTTGGGTCATTAAACTCCATAAATCATTAATTTTAATTTTTTTTCTAGAATATTATATCACATGTGAGAGTGAAGATTTAAGCCTACCATTTCAAAACGTAATTGATCAAATTCATATGTTAGGCTACACAAAGATGAACAAAACTTAAGATGCAACAAGTATTTTTCTTAATTATTTTTTCATCTCTGCAAAAATATTATTGTTTTGGAAATCACTATATTATTGGCACAAAAAATGTTACGTATATTAAAGAATAAAAAATATTAAAATTACAAATTTAATCTATGAACTTTTAGATTTGTGTCTATTTGATTCTTGAATTTTAGAGAGTGTCTCATCTTTGAACTTATATATATATTTTTTAAATATAGGTTTAAAGGTCATTGACATTTTTGGAATTTTTAAAAATTAACCAATATGCACTATTAGATATAGAATTGAATTTAATAGGTTCAAAAACACTTTTGGTCCCTAATATAGGATGAAAGTAACAATTTAGTTTTAGTCTGTAACAACTTTGTTCCTCTACTTTTAATTTTGTAATAATTTAGTCTCTGAACTTTAGTAGGTAACAATTTAGTCACTATACTTATAAATTTGTAAGAATTTAATCCCTGCCCTAAAAAAATTCATTAAAATTAAATGTCAAATTTTATAATTTTAAGGATGTAGACTTTGTAGCTTAGAAATATATTGGAGTCCCTAATTAGTTTATTTATCTACCTATACTAGAAATTTAACATAGCCCACTTGGTCAAGACATGACTTTTAACTTAAGTTGAAAAAACGGGATGTTCTAAATCTGTTTGTTTTATAATTATGATGTATCTATCATTTTAGTCAATTGGAAGACTTTTCTCTAGCCTCTTCCCGTAACTTATACAATCAACTTTGAGCTTTTCTCTAGTCACTACTATATACTTTCTTTTGGCTATTGGTCTGGAATATCATGAGTGTGCTACAAATGTGTTAACCTAGTTGAGATATCTTAGTGCGTTAAAAGAAAAGTTAAAAAAAGGGGTATTGGTTTTGATTTATCTTCTTTTTCCATGAAACTGAATAATGATTGACAGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGATAACAAAAATGGTATTTGAAAGCTATGGAGTATCGAAGGTTGAGAGTGAGTCAAACATGGAACCCACAAATTACCTTCTTCGATTCTTCAAATACAGAGTGCCTGAGAAAGATGAAACCGATCTGGGATTGCATTCCCATACAGATCTCACTTTCTTTTCCCTAATTCATCAGCATCTCATCTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGATCGATGTTCAACCCTCAGGTCATTGCTCCTTCGTTGTCATGGCTGGTGATGCACTCATGGTAACCATTACCAATCCCTTTTTCTTCTCCTTAACAAAATTTGGACTGCAAACAAATGATTATTAAAACATATGCTAAAATATAAATCTCAAATTACAAATTTACTTGGTAAACTTTATAAGAGATTTGTAACTTTTTATTTTTTTAAAAAAGGTTAAATTATCAAAAATATCCATGAAGGTGTTAGTTTGAACTATACCATGAACTTTTAAGAATTTTATTTTAATTCTTATTAAAGTTTGAAGTTTATTTTAAAACAACTCTAGTAATTAAGCTTTTGTAGTTAAATTCATAGATAAATGTTTGTACTTGCTTATGTGAACAAATTTAAATGCATACATATAAGACAAATAAAGACACACGACACATGTGTGGAACATATCGTCTTCCCTCCTTTCAATCTCTCAAATGCCCAAATTTTCATTCTTTCTTTTATAATTTTTTTTTCGCTAACTCCTAAATAGGGGTGTAGATAAGTTGGGTTGGAGAGGTTTTTTAGACCAATTCGAAATTTTTTTTATTTATTTAACCCAAAAAAATAAAAAAAAATTAAAACCCAACCTAATTCAATAACAAAACTAACCCAACACAACTCTTATAATTTGGGTTGGGTAGTTTGGGTTGTTCGGGTAATTGGTCATTTGAATATCCCTACCCTCAAATTAGTTATGATATGTGTTTAATTAAATTTGTCCACTTAAGAAACACATAAGTAGACACAAACTTTTTCATCTCATTCATCAATTTAACGTGAAAAGCCTTCAATGATTGATTTTTGAAAAAAAAAAAAAACCTCAAACTTGAAGGATTAAAATAAAACGTTTCAAAGTTTAGGAATATAGAATTGAAACAAATGATCCCATACTTGATAAGAGGTATTTTTTTAAAAAAAAATTTACGTTAGACCATAAACTTATAAATGTCAATTTCAAAATGAGTGACTAAACTTGTAATGTAACATTATGTTTTTGTATATTTATTGATGGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCATCGAGTGACAATGAGAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGGAACCATTGATGTGCCTAAAGAGCTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCAAATTCTTCTCATAAAACTATCTCTCGCATCAAAGATTATTGTGGCCTTTAA

mRNA sequence

ATGGTTAATTCTGAAGCAAAAGCCAAAGTCCCCATAATAGATTTGTCAAAATTAGAGGCAGCTGGCACAGAAGAATGGGGCTTAACATGCAAAGAGATGAGGCTTGGTTTAGAAGAATTTGGTTGCTTTACAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCACAACTCAATTTTTGAAGAAACAAAAGAACTTTTTCATCTCCCAATTGAAACTAGAATAAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGACAATCCAACTCACCTACAACCAACTCACAGTTTTGCTAATCTCATGTGGCCTGCTCGTCCAAACAATCACTTCTGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGATAACAAAAATGGTATTTGAAAGCTATGGAGTATCGAAGGTTGAGAGTGAGTCAAACATGGAACCCACAAATTACCTTCTTCGATTCTTCAAATACAGAGTGCCTGAGAAAGATGAAACCGATCTGGGATTGCATTCCCATACAGATCTCACTTTCTTTTCCCTAATTCATCAGCATCTCATCTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGATCGATGTTCAACCCTCAGGTCATTGCTCCTTCGTTGTCATGGCTGGTGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCATCGAGTGACAATGAGAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGGAACCATTGATGTGCCTAAAGAGCTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCAAATTCTTCTCATAAAACTATCTCTCGCATCAAAGATTATTGTGGCCTTTAA

Coding sequence (CDS)

ATGGTTAATTCTGAAGCAAAAGCCAAAGTCCCCATAATAGATTTGTCAAAATTAGAGGCAGCTGGCACAGAAGAATGGGGCTTAACATGCAAAGAGATGAGGCTTGGTTTAGAAGAATTTGGTTGCTTTACAGTGGTTTATGACAAAGTTTCTTTGGAGCTTCACAACTCAATTTTTGAAGAAACAAAAGAACTTTTTCATCTCCCAATTGAAACTAGAATAAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGACAATCCAACTCACCTACAACCAACTCACAGTTTTGCTAATCTCATGTGGCCTGCTCGTCCAAACAATCACTTCTGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGATAACAAAAATGGTATTTGAAAGCTATGGAGTATCGAAGGTTGAGAGTGAGTCAAACATGGAACCCACAAATTACCTTCTTCGATTCTTCAAATACAGAGTGCCTGAGAAAGATGAAACCGATCTGGGATTGCATTCCCATACAGATCTCACTTTCTTTTCCCTAATTCATCAGCATCTCATCTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGATCGATGTTCAACCCTCAGGTCATTGCTCCTTCGTTGTCATGGCTGGTGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCTTGTAGGCATCGAGTGACAATGAGAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGGAACCATTGATGTGCCTAAAGAGCTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCAAATTCTTCTCATAAAACTATCTCTCGCATCAAAGATTATTGTGGCCTTTAA

Protein sequence

MVNSEAKAKVPIIDLSKLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSHKTISRIKDYCGL

Homology

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BLAST of HG10023307 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 570.5 bits (1469), Expect = 9.2e-159
Identity = 268/316 (84.81%), Postives = 289/316 (91.46%), Query Frame = 0


Query: 1   MVNSEAKAKV-PIIDLSKLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIF 60
           MVNSEAKAKV P IDLSKLEA GTEEWGL CKEMRLGLEEFGCF+VVYD+VSLELHNSIF
Sbjct: 1   MVNSEAKAKVLPTIDLSKLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSIF 60

Query: 61  EETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 120
           E TKELFHLP+ETRIKNTSEKPYHGYFGGYS LPLYESM IDNP  LQ T SFANLMWP 
Sbjct: 61  EATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWPT 120

Query: 121 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEK 180
           + NNHFC TVERFSTLVA+LEKM+TKMV ESYG+SK+ +E NME  NYLLR FKYR PEK
Sbjct: 121 KENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPEK 180

Query: 181 DETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSN 240
           DETD+GLHSHTDLTFFS+IHQHLISGLQ+Q LDDQWID+QPSGHC F+VMAGDALMAWSN
Sbjct: 181 DETDVGLHSHTDLTFFSIIHQHLISGLQVQSLDDQWIDIQPSGHCYFIVMAGDALMAWSN 240

Query: 241 ERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKA 300
           ERIRSCRHRV MRGEETRYSIG+FSFK+GTI VP+ELVDDANPLRYKPFNHYDFLTYDKA
Sbjct: 241 ERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFLTYDKA 300

Query: 301 NSSHKTISRIKDYCGL 316
            +SH+ +SRIKDYCG+
Sbjct: 301 KASHQNVSRIKDYCGI 316

[Close]

BLAST of HG10023307 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 551.6 bits (1420), Expect = 4.4e-153
Identity = 267/314 (85.03%), Postives = 283/314 (90.13%), Query Frame = 0


Query: 5   EAKAKV--PIIDLSKLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEET 64
           EAK+KV  P IDLSKLE  GT+EWGL CKEMR GLEEFGCF+VVYDKVSLELHNSI E T
Sbjct: 4   EAKSKVVIPTIDLSKLE-VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 65  KELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPN 124
           KELFHLPIETR+KNTSEKPYHGYFGGYSFLPLYESMAIDNP  L+ T SFANLMWP R N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123

Query: 125 NHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDET 184
           NHFC TVERFSTLVAKLEKM+TKMV ESYGVSKV +E  ME TNYLLR FKYRV EKDET
Sbjct: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183

Query: 185 DLGLHSHTDLTFFSLIHQHLISGLQIQLL-DDQWIDVQPSGHCSFVVMAGDALMAWSNER 244
           D+GLHSHTDLTFFS+IHQHLISGLQIQ L DDQW+DV PS HCSF+VMAGDALMAWSNER
Sbjct: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243

Query: 245 IRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANS 304
           IRSCRHRV MRGEETRYSIG+FSFK+GTI+VP+  VDDANPLRYKPFNHYDFLTYDKAN+
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303

Query: 305 SHKTISRIKDYCGL 316
           SH TISRIKDYCGL
Sbjct: 304 SHNTISRIKDYCGL 316

[Close]

BLAST of HG10023307 vs. NCBI nr
Match: XP_004136073.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus])

HSP 1 Score: 548.5 bits (1412), Expect = 3.7e-152
Identity = 265/314 (84.39%), Postives = 282/314 (89.81%), Query Frame = 0


Query: 5   EAKAKV--PIIDLSKLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEET 64
           EAK+KV  PIIDLSKLE  GT+EWGL C+EMR GLE+FGCF+VVYDKVSLELHNSIFE T
Sbjct: 4   EAKSKVVIPIIDLSKLE-EGTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 65  KELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPN 124
           KELFHLPIETR+KNTSEKPYHGYFGGYSFLPLYESMAIDNP  LQPT SFANLMWP   N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTEN 123

Query: 125 NHFCETVERFSTLVAKLEKMITKMVFESYGVS-KVESESNMEPTNYLLRFFKYRVPEKDE 184
           NHFC  VERFSTLVAKLE M+TKMV ESYGVS KV SE  ME TNYLLR FKYRV EKDE
Sbjct: 124 NHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKDE 183

Query: 185 TDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNER 244
            D+GLHSHTDLTF S+IHQHLISGLQIQLLDDQW++V PS HCSF VMAGDALMAWSNER
Sbjct: 184 NDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNER 243

Query: 245 IRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANS 304
           IRSCRHRV MRGEETRYSIG+FSFK+G I+VP+E VDDANPLRYKPF+HYDFLTYDKAN+
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKANA 303

Query: 305 SHKTISRIKDYCGL 316
           SHKTISRIKDYCGL
Sbjct: 304 SHKTISRIKDYCGL 316

[Close]

BLAST of HG10023307 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 4.7e-139
Identity = 237/316 (75.00%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLEA--AGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIF 61
           + SE +A+VP+IDLSKLE   AGTE WG TCK MR+GLEEFGCF VV+++VSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 62  EETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 121
               ELF LPIETRIKNTSEK YHGYFG YSFLPLYESMAI+NPT L    SFANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 122 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEK 181
           + NNHF ET++RFSTL+AKL+K+IT+MV ESYGV+K   ++N++ T  LLRFFKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 182 DETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSN 241
            ETD+GLH+HTDLTFFS+IHQHLI GLQIQ LDDQWID++PSGHCSFVVMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 242 ERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKA 301
           +RIRSCRHRVTM+ EETRYS G+FSFK+GTI VP+ELVDDANPLRYKPFNH+DFLTYDKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 302 NSSHKTISRIKDYCGL 316
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of HG10023307 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-136
Identity = 234/316 (74.05%), Postives = 269/316 (85.13%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLE--AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIF 61
           + SE +A VP+IDLSKLE   AGT+ WG TCK MR+GLEEFGCF VV+++VSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 62  EETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 121
               ELF LPIETRIKNTSEK YHGYFG YSFLPLYESMAIDNPT L    SFANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 122 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEK 181
           + NN F ET++RFSTLVAKL+K++T+MV ESYGVSK   ++N++ T  LLRFFKYRVPE+
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 182 DETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSN 241
            ETD+GLH+HTDLTFFS+IHQHLI GLQIQ LDDQWID++PS HCSF+VMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 242 ERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKA 301
           +RIRSCRHRVTM+ EETRYS G+FSFK+GTI VP+ELVDDANPLRYKPFNHYDFLT+DKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 302 NSSHKTISRIKDYCGL 316
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of HG10023307 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.3e-58
Identity = 121/296 (40.88%), Postives = 177/296 (59.80%), Query Frame = 0


Query: 9   KVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++P+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FE  ++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 69  LPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCE 128
           LPI T+ +N S KP+HGY        LYES+ ID+   L+  + F   +WP   N    E
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 129 TVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKD-----ET 188
           T+  FS  + +L+ M+ +M+ ES+G+     E ++  T YL R  KY  P  D     ET
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 189 DLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERI 248
            LGL SHTD    +++HQ+ + GL+++  DD+WI V+PS   S +VM GD+L A  N R+
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRL 252

Query: 249 RSCRHRVTMRGEETRYSIGLFSF-KSGT-IDVPKELVDDANPLRYKPFNHYDFLTY 297
            S  HRV M G++TRYS GLFS  K+G  ID P+ELVD  +P  +KPF + DFL +
Sbjct: 253 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 302

[Close]

BLAST of HG10023307 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.6e-57
Identity = 120/297 (40.40%), Postives = 177/297 (59.60%), Query Frame = 0


Query: 9   KVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++P+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FE  ++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 69  LPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCE 128
           LPI T+ +N S KP+HGY        LYES+ I++   L+  + F   +WP   N    E
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 129 TVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKD------E 188
           T+  FS  + +L+ M+ +M+ ES+G+     E ++  T YL R  KY  P  D      E
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 189 TDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNER 248
           T LGL SHTD    +++HQ+ + GL+++  DD+WI V+PS   S +VM GD+L A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGR 252

Query: 249 IRSCRHRVTMRGEETRYSIGLFSF-KSGT-IDVPKELVDDANPLRYKPFNHYDFLTY 297
           + S  HRV M G++TRYS GLFS  K+G  ID P+ELVD  +P  +KPF + DFL +
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 303

[Close]

BLAST of HG10023307 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.6e-57
Identity = 120/295 (40.68%), Postives = 177/295 (60.00%), Query Frame = 0


Query: 9   KVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++P+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FE  ++LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 69  LPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCE 128
           LPI T+ +N S K +HGY        LYES+ I++   L+  + F   +WP   N    E
Sbjct: 66  LPIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 129 TVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKD----ETD 188
           T+  FS  + +L+ M+ +M+ ES+G+ K   E ++  T YL R  KY  P  D    ET 
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDE-HLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 189 LGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIR 248
           LGL SHTD    +++HQ+ + GL+++  DD+WI V+PS   S +VM GD+L A  N R+ 
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRLH 245

Query: 249 SCRHRVTMRGEETRYSIGLFSF-KSGT-IDVPKELVDDANPLRYKPFNHYDFLTY 297
           S  HRV M G++TRYS GLFS  K+G  ID P+ELVD  +P  +KPF + DFL +
Sbjct: 246 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 294

[Close]

BLAST of HG10023307 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 7.0e-53
Identity = 115/312 (36.86%), Postives = 168/312 (53.85%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLS-KLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFE 61
           + S A  ++P+IDLS K    GT  W     ++R  LEE+GCF  V D VS EL  ++  
Sbjct: 1   MGSTAPLRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCN 60

Query: 62  ETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPAR 121
           +  EL +LP+ET++ N ++  Y G+    S   L E M +   T L+    F NLMWP  
Sbjct: 61  KGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWP-E 120

Query: 122 PNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKD 181
            N  FCETV  +   +A+++ ++  MVFES+G+ K   +S  E TNYLL F +Y+ P KD
Sbjct: 121 GNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEK-HFDSFCESTNYLLHFMRYQQPGKD 180

Query: 182 ETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNE 241
                L  H D +  ++++Q+ + GL+ +  D +WI      H   +V+ GD  MAWSN 
Sbjct: 181 GRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWILPTADNH---IVLLGDCFMAWSNG 240

Query: 242 RIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKAN 301
           R+ S  HRVT+   + R S   FSF    I+ P ELVD+ +PL + PF   + L Y    
Sbjct: 241 RLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTK 300

Query: 302 SSHKTISRIKDY 313
              K +  +K Y
Sbjct: 301 EGAKAVCDLKQY 304

[Close]

BLAST of HG10023307 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 5.2e-40
Identity = 113/428 (26.40%), Postives = 186/428 (43.46%), Query Frame = 0


Query: 9   KVPIIDLS-KLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++P+I+L+ K    G+ +W     ++R  LE+FGCF   YDKVSLEL  SI +  +ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 69  LPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWP-ARPNNHFC 128
           LP+ET+ +N   KPY GY    +   L ES+ I N   L+  + F   +WP    N +  
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGNENIS 126

Query: 129 ETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVP--------- 188
           +T++ F+  + +++ M+ +MV ES+G+ K   + +++ T Y +R  KY  P         
Sbjct: 127 KTIQLFAEKLVEIDVMVRRMVMESFGIEKY-IDDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 189 ------------------------------------------------------------ 248
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 249 -----------------------------------------------EKDETDLGLHSHT 308
                                                          + +E  LGL  HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 309 DLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCRHRVT 316
           D   F+++ QH I GL+++  D++WI V+PS + +F+V+AGD+L A  N RIR+  HRV 
Sbjct: 307 DKNLFTVLFQHEIEGLEVKTKDEKWIRVKPSPN-TFIVIAGDSLCALMNGRIRAPYHRVR 366

[Close]

BLAST of HG10023307 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.1e-153
Identity = 267/314 (85.03%), Postives = 283/314 (90.13%), Query Frame = 0


Query: 5   EAKAKV--PIIDLSKLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEET 64
           EAK+KV  P IDLSKLE  GT+EWGL CKEMR GLEEFGCF+VVYDKVSLELHNSI E T
Sbjct: 4   EAKSKVVIPTIDLSKLE-VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 65  KELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPN 124
           KELFHLPIETR+KNTSEKPYHGYFGGYSFLPLYESMAIDNP  L+ T SFANLMWP R N
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDN 123

Query: 125 NHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDET 184
           NHFC TVERFSTLVAKLEKM+TKMV ESYGVSKV +E  ME TNYLLR FKYRV EKDET
Sbjct: 124 NHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDET 183

Query: 185 DLGLHSHTDLTFFSLIHQHLISGLQIQLL-DDQWIDVQPSGHCSFVVMAGDALMAWSNER 244
           D+GLHSHTDLTFFS+IHQHLISGLQIQ L DDQW+DV PS HCSF+VMAGDALMAWSNER
Sbjct: 184 DVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNER 243

Query: 245 IRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANS 304
           IRSCRHRV MRGEETRYSIG+FSFK+GTI+VP+  VDDANPLRYKPFNHYDFLTYDKAN+
Sbjct: 244 IRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANA 303

Query: 305 SHKTISRIKDYCGL 316
           SH TISRIKDYCGL
Sbjct: 304 SHNTISRIKDYCGL 316

[Close]

BLAST of HG10023307 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 2.3e-139
Identity = 237/316 (75.00%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLEA--AGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIF 61
           + SE +A+VP+IDLSKLE   AGTE WG TCK MR+GLEEFGCF VV+++VSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 62  EETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 121
               ELF LPIETRIKNTSEK YHGYFG YSFLPLYESMAI+NPT L    SFANLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 122 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEK 181
           + NNHF ET++RFSTL+AKL+K+IT+MV ESYGV+K   ++N++ T  LLRFFKYRVPE+
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 182 DETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSN 241
            ETD+GLH+HTDLTFFS+IHQHLI GLQIQ LDDQWID++PSGHCSFVVMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240

Query: 242 ERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKA 301
           +RIRSCRHRVTM+ EETRYS G+FSFK+GTI VP+ELVDDANPLRYKPFNH+DFLTYDKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300

Query: 302 NSSHKTISRIKDYCGL 316
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of HG10023307 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 6.2e-137
Identity = 234/316 (74.05%), Postives = 269/316 (85.13%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLE--AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIF 61
           + SE +A VP+IDLSKLE   AGT+ WG TCK MR+GLEEFGCF VV+++VSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 62  EETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 121
               ELF LPIETRIKNTSEK YHGYFG YSFLPLYESMAIDNPT L    SFANLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 122 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEK 181
           + NN F ET++RFSTLVAKL+K++T+MV ESYGVSK   ++N++ T  LLRFFKYRVPE+
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 182 DETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSN 241
            ETD+GLH+HTDLTFFS+IHQHLI GLQIQ LDDQWID++PS HCSF+VMAGDALMAWSN
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSSHCSFIVMAGDALMAWSN 240

Query: 242 ERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKA 301
           +RIRSCRHRVTM+ EETRYS G+FSFK+GTI VP+ELVDDANPLRYKPFNHYDFLT+DKA
Sbjct: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLTHDKA 300

Query: 302 NSSHKTISRIKDYCGL 316
             S K + RI+DYC +
Sbjct: 301 KGSKKFLYRIRDYCSI 316

[Close]

BLAST of HG10023307 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.1e-130
Identity = 227/319 (71.16%), Postives = 270/319 (84.64%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFE 61
           + ++AK+K+P I+LS+ +   G+EEW L  KE+R+ LEEFGCF VVY+KVSLELHNSIFE
Sbjct: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60

Query: 62  ETKELFHLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPAR 121
            TKELFHLPIETRI+NTSEKPYHGYFG YSFLPLYESMA+DNPT LQ   +F NLMWPA 
Sbjct: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA- 120

Query: 122 PNNHFCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKD 181
            N  FCETV  FSTLVAKLE+ +T+MV ESYGVSK   ES++E T +LLR FKYRVP++D
Sbjct: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD 180

Query: 182 ETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDD----QWIDVQPSGHCSFVVMAGDALMA 241
           ETDLGLHSHTDLTF S++HQHLI GLQ++ LD+    +WID+ P G  SF+VMAGDALMA
Sbjct: 181 ETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMA 240

Query: 242 WSNERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTY 301
           WSN+RIR CRHRV MRG+ETRYS+GLFSFKSGTI VP+ELV+D +PL+YKPF+H++FL Y
Sbjct: 241 WSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRY 300

Query: 302 DKANSSHKTISRIKDYCGL 316
           DKAN+SHKT SRIKDYCG+
Sbjct: 301 DKANASHKTCSRIKDYCGI 318

[Close]

BLAST of HG10023307 vs. ExPASy TrEMBL
Match: A0A5A7USF0 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002400 PE=3 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.2e-119
Identity = 207/243 (85.19%), Postives = 221/243 (90.95%), Query Frame = 0


Query: 74  IKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCETVERFS 133
           +KNTSEKPYHGYFGGYSFLPLYESMAIDNP  L+ T SFANLMWP R NNHFC TVERFS
Sbjct: 1   MKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRDNNHFCVTVERFS 60

Query: 134 TLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDLGLHSHTDLT 193
           TLVAKLEKM+TKMV ESYGVSKV +E  ME TNYLLR FKYRV EKDETD+GLHSHTDLT
Sbjct: 61  TLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDETDVGLHSHTDLT 120

Query: 194 FFSLIHQHLISGLQIQLL-DDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCRHRVTMR 253
           FFS+IHQHLISGLQIQ L DDQW+DV PS HCSF+VMAGDALMAWSNERIRSCRH+V MR
Sbjct: 121 FFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNERIRSCRHKVIMR 180

Query: 254 GEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSHKTISRIKDY 313
           GEETRYSIG+FSFK+GTI+VP+  VDDANPLRYKPFNHYDFLTYDKAN+SH TISRIKDY
Sbjct: 181 GEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRIKDY 240

Query: 314 CGL 316
           CGL
Sbjct: 241 CGL 243

[Close]

BLAST of HG10023307 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 9.4e-77
Identity = 137/309 (44.34%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 9   KVPIIDLSKLE--AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELF 68
           KVPI+DL+  +     T  W    +E    LEE+GCF  VYD V+ +L +SIF   +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 69  HLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFC 128
            LP ET+ KN +EKPYHGY G    +PL+E + +D  T+ +    F +LMWP + N+ FC
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFC 128

Query: 129 ETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDLGL 188
            TV  FS  VA+L++++ +M+FE+YGV K   ES++    YLL+F KY  P +  +    
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEK-HYESHVGSKTYLLKFLKYLAPPESISMPAF 188

Query: 189 HSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCR 248
             HTD TF S++HQ+ ++GL+++  D +WI +Q     S+VVMAGD  M WSN+RIRSC 
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 248

Query: 249 HRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSHKTI 308
           HRVTM G++TRY++GLFSF +  + +P+ELVDD +PL YKPF++   + +       +  
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 309 SRIKDYCGL 316
           S +K YCG+
Sbjct: 309 STLKAYCGI 314

[Close]

BLAST of HG10023307 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.1e-72
Identity = 131/308 (42.53%), Postives = 202/308 (65.58%), Query Frame = 0


Query: 9   KVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTV-VYDKVSLELHNSIFEETKELF 68
           K+P +D S+ +   GT+ W  T + +R  LEE+GCF + + DK  L+L + +F    +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 69  HLPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFC 128
            LP +T++KN  +KP +GY G    LPL+ES+ IDN T L+ T SF  LMWP + N HF 
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHFS 123

Query: 129 ETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDLGL 188
           E + +++   A+L++M+T+MVF+SY V K   +  +E T YLLR  K R P  +   LG 
Sbjct: 124 ECLYKYAEFAAELDQMVTRMVFQSYNVEKY-YDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 189 HSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCR 248
            +HTD +F +++HQ  ++GL+++  + + I++  S    F+V+AGDALMAWSN+R+ S R
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 243

Query: 249 HRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSHKTI 308
           H+V + GE  RYS+G+F+F +GT+ VP+EL+D  +PL YKPF+H   L + + ++ +K+ 
Sbjct: 244 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 303

Query: 309 SRIKDYCG 315
             IK YCG
Sbjct: 304 CPIKAYCG 309

[Close]

BLAST of HG10023307 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 253.8 bits (647), Expect = 1.8e-67
Identity = 126/310 (40.65%), Postives = 197/310 (63.55%), Query Frame = 0


Query: 9   KVPIIDLS-KLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++P+ID S K    G  EW LT  +++  L+++G F   +D++  EL  S+F   +ELF 
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 69  LPIETRIKNTSEKPYHGYFGGYSFLPLYESMAIDNPTHLQPTHSFANLMWPARPNNHFCE 128
           LP++T+++N S+KP+HGY G Y  +PLYESM ID+    +   +F   +WP + N  F  
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWP-QGNISFST 130

Query: 129 TVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDLGLH 188
           T++ FS  +++L+  I +M+ ES+G+ K   E ++  TNYLLR  KY+ P+ +ET +GL+
Sbjct: 131 TIQSFSKKLSELDITIRRMIMESFGLDKYIDE-HLHSTNYLLRVMKYKGPDTEETKVGLN 190

Query: 189 SHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWSNERIRSCRH 248
           +HTD    ++++Q+ + GL++Q  D  WI V+P+   SF VM GD+L A  N R+ S  H
Sbjct: 191 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPT-QDSFTVMIGDSLYALLNGRLHSPYH 250

Query: 249 RVTMRGEETRYSIGLFSF-KSG-TIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSHKT 308
           RV M G ETRYS+GLFS  K+G  +  P ELVD+ +P  +KPF+H +FL +    +  ++
Sbjct: 251 RVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRS 310

Query: 309 ISRIKDYCGL 316
            S +K YCG+
Sbjct: 311 QSALKTYCGI 317

[Close]

BLAST of HG10023307 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.9e-61
Identity = 124/320 (38.75%), Postives = 190/320 (59.38%), Query Frame = 0


Query: 2   VNSEAKAKVPIIDLSKLE-AAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFE 61
           ++S  +  +PI+D S  +   GT  W  T  ++R  +E  G F   +  VS +L +++  
Sbjct: 1   MDSHEEQCLPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLA 60

Query: 62  ETKELFHLPIETRIKNTSEKPYHGYFG-GYSFLPLYESMAIDNPTHLQPTHSFANLMWPA 121
             KE+++LP + +IKN + K  HGY         ++ES+ ID  T LQ    F+ L+WP 
Sbjct: 61  GMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP- 120

Query: 122 RPNNHFCETVERFSTLVAKLEKMITKMVFESYGV-SKVESESNMEPTNYLLRFFKYRVPE 181
           + N+ FC+T   ++  +A+L++ + +M++ESYG+  K  S S+ E T YLLR   YR  +
Sbjct: 121 QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQ 180

Query: 182 KDETDLGLHSHTDLTFFSLIHQHLISGLQIQLLDDQWIDVQPSGHCSFVVMAGDALMAWS 241
             E + G  SHTD +F S++HQ+ + GLQ++ +  QW+   PS    FVV++G  L AWS
Sbjct: 181 NGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPS-PTRFVVLSGMGLTAWS 240

Query: 242 NERIRSCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLT-YD 301
           N+RI++C H+V M  +E RYS+G FSF  GTI  P+ELVDD +PLRY PF H   L  Y+
Sbjct: 241 NDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYE 300

Query: 302 KANSSHKTISR--IKDYCGL 316
              +S K  S   ++ YCG+
Sbjct: 301 SYINSLKKSSEDLLQIYCGV 318

[Close]

BLAST of HG10023307 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 1.9e-61
Identity = 130/314 (41.40%), Postives = 180/314 (57.32%), Query Frame = 0


Query: 9   KVPIIDLS-KLEAAGTEEWGLTCKEMRLGLEEFGCFTVVYDKVSLELHNSIFEETKELFH 68
           ++PII+LS K     TE W  T   +R  +E  G F   Y+     LH SI E  KEL  
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLD 67

Query: 69  LPIETRIKNTSEKPYHGYFGGYS-FLPLYESMAIDNPTHLQPTHSFANLMWP--ARPNNH 128
           LP+E ++KN + K  HGY    S   P++E + ID    +Q    F+ LMWP     N+ 
Sbjct: 68  LPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDNDR 127

Query: 129 FCETVERFSTLVAKLEKMITKMVFESYGVSKVESESNMEPTNYLLRFFKYRVPEKDETDL 188
           FCETV  ++ + A+LE+++ +M+FESY V K  +E  +  T YLLR  KYR     E + 
Sbjct: 128 FCETVHAYAKMQAELEQLVIRMLFESYNVEKY-TEKYIGGTRYLLRLLKYRRLPNGEPNR 187

Query: 189 GLHSHTDLTFFSLIHQHLISGLQIQL-LDDQWIDVQPSGHCSFVVMAGDALMAWSNERIR 248
              SHTD +F S++HQ+ I+GL ++   +D W    PS    FVV+AGDA+MAWSN+RI+
Sbjct: 188 KFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPS-PTRFVVIAGDAIMAWSNDRIK 247

Query: 249 SCRHRVTMRGEETRYSIGLFSFKSGTIDVPKELVDDANPLRYKPFNHYDFLTYDKANSSH 308
           +C H+V M   E RYS+G FSF+ G I  P+E+VD  +PL Y PF+H   L Y +    H
Sbjct: 248 ACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVH 307

Query: 309 KTISR--IKDYCGL 316
               R   K YCG+
Sbjct: 308 LKAHRTMTKAYCGI 319

The following BLAST results are available for this feature:

BLAST of HG10023307 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898684.1	9.2e-159	84.81	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_008461057.1	4.4e-153	85.03	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi...	[more]
XP_004136073.1	3.7e-152	84.39	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis sativus]	[more]
XP_022992484.1	4.7e-139	75.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima]	[more]
XP_022953943.1	1.3e-136	74.05	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]

BLAST of HG10023307 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	4.3e-58	40.88	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	1.6e-57	40.40	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	3.6e-57	40.68	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	7.0e-53	36.86	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	5.2e-40	26.40	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of HG10023307 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CDV2	2.1e-153	85.03	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX...	[more]
A0A6J1JXN6	2.3e-139	75.00	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GPR2	6.2e-137	74.05	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1CXE5	1.1e-130	71.16	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]
A0A5A7USF0	1.2e-119	85.19	Putative 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo va...	[more]

BLAST of HG10023307 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	9.4e-77	44.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	1.1e-72	42.53	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.8e-67	40.65	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	1.9e-61	38.75	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	1.9e-61	41.40	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..86 e-value: 6.5E-11 score: 43.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..265 e-value: 2.2E-16 score: 60.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..304 e-value: 5.4E-61 score: 208.7
None	No IPR available	PANTHER	PTHR47990:SF169	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE	coord: 7..314
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 7..314
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 8..296
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 164..268 score: 10.707326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10023307.1	HG10023307.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding