HG10023273 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023273
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRHOMBOID-like protein
LocationChr05: 32657191 .. 32659012 (-)
RNA-Seq ExpressionHG10023273
SyntenyHG10023273
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGAGACCGCCCTCGGTCAACATCGAGTCGAAGTCCTATGACGTCCACAAGGCCATCTCAATGCCGCCGCCACCGAGCTATTATCCTCCCCCGCCCCAGCCATGGTTCCCATGGCTGGTGCCTTTCATTTTTACAGCCAATGTGGCCATGTTTGTTTTCACATTGTATGAAAATAATTGCCCCGCCACCACCGGAGCAAACCACTGCCTTCTTTATCCCGAACTTGGCCGGTTCTCGTTCCAACCGTTTCAGGAGAATCCCCTCCTCGGCCCTTCCATCAACACGTAAGAATCAAAACCCTCTTTCCAGCAAGAGATATTATTTGAGGGACTAGAACAATCAAACAATGTGGTTGACGATTCTATGAGAATTTTTACAAACGGTTTATTTCATTAGAGCGTTGGTCTAATTGAATAATTATTCCTTTATATGGAAAATTAGCATATGGGTTTGTGAATGAGGATGAAATTGGATAACCCATCAAGAGAAAAAATGAAAAAAAAAAAAAAAAATCTGGGTTTTGCAGAATTAGGGGGGAAAACAGAGGGAATTCTGTTTATTTATTATGGTTTGTTTGATTTGTTTTGTTTTATGTTTTTGTCAAAATGGTGTTGCAGTTTGAAAAGATATGGGGCTTTGGACAAAGAAGCAGTAGTAGAACATGGGGAAGGGTGGCGCTTGGTGTCTTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGCCTCGAACAAGAATTTGGATTTTGTAAGCACTTTCCAAACATATAAGACCTATTTGAAATGACTTTTCAAGTGCTTAAAAACACTTTTTTCAAGAACTTAAAAAGTGATTCCAAACAAACTCATAATCTGTTATGTATTGATTCATTGTTTCACTATTCTTAGCATTATTTGTCTGAATTTTAAAAAGGAAAAAAGGGGTTAAAATCATCTGTTATGTTGATTTTAACTCTTTCAAGATCATTCTTAAACAGCATTTGATCACTGTCAAACATGTTTGAAGTCATCTCAAACTTGATCATAGTGTATTTATAATGTTTTAAATTTCTCAAAACCCCATTTGGGATTTGGTTTTTGTTTGAGTGTTTTTTGGTTTTGGCAGTGAAAATAGGATGTTTATATGTGCTTTCTGGATTTGGTGGGAGTTTACTTTCAGCTCTAAGTATCAACCCAGCTGAAAATCCAACCATATCAGTTGGTGCTTCAGGTGCACTTTTTGGGCTTTTGGGAGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATGCAAATAAGGTACCAAAAAGCAAAAAAAAAAAAAAAAACTTCCTTCCTTTTTTCCATTGTTCTATAAACATTTTTGAACTTATGACTTGTTTGTCAATGAAACTCAGTTTGCAGCCCTGACGTCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTTGTACCACACGTTGACAATTCCGCCCACATCGGAGGCTTCATTTCGGGCTTTCTCCTCGGCTTCATCCTCTTAATCCGACCGCAATTCGGATACGTTAACCACAAGTACATCCCTGCAGGGTCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATTATAGCTCTACTGCTCTTGATTTTCGGGTAATCGCAACGACGATATATAATTTTCGGTCTTGTTGGAGTTCTTTTTTCGTTAACTTTTGTCACTTGTATTTATGCAGATATGCTTCTGGTTTAACAAAGCTGTACAGAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGA

mRNA sequence

ATGGGGAAGAGACCGCCCTCGGTCAACATCGAGTCGAAGTCCTATGACGTCCACAAGGCCATCTCAATGCCGCCGCCACCGAGCTATTATCCTCCCCCGCCCCAGCCATGGTTCCCATGGCTGGTGCCTTTCATTTTTACAGCCAATGTGGCCATGTTTGTTTTCACATTGTATGAAAATAATTGCCCCGCCACCACCGGAGCAAACCACTGCCTTCTTTATCCCGAACTTGGCCGGTTCTCGTTCCAACCGTTTCAGGAGAATCCCCTCCTCGGCCCTTCCATCAACACTTTGAAAAGATATGGGGCTTTGGACAAAGAAGCAGTAGTAGAACATGGGGAAGGGTGGCGCTTGGTGTCTTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGCCTCGAACAAGAATTTGGATTTTTGAAAATAGGATGTTTATATGTGCTTTCTGGATTTGGTGGGAGTTTACTTTCAGCTCTAAGTATCAACCCAGCTGAAAATCCAACCATATCAGTTGGTGCTTCAGGTGCACTTTTTGGGCTTTTGGGAGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATGCAAATAAGTTTGCAGCCCTGACGTCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTTGTACCACACGTTGACAATTCCGCCCACATCGGAGGCTTCATTTCGGGCTTTCTCCTCGGCTTCATCCTCTTAATCCGACCGCAATTCGGATACGTTAACCACAAGTACATCCCTGCAGGGTCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATTATAGCTCTACTGCTCTTGATTTTCGGATATGCTTCTGGTTTAACAAAGCTGTACAGAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGA

Coding sequence (CDS)

ATGGGGAAGAGACCGCCCTCGGTCAACATCGAGTCGAAGTCCTATGACGTCCACAAGGCCATCTCAATGCCGCCGCCACCGAGCTATTATCCTCCCCCGCCCCAGCCATGGTTCCCATGGCTGGTGCCTTTCATTTTTACAGCCAATGTGGCCATGTTTGTTTTCACATTGTATGAAAATAATTGCCCCGCCACCACCGGAGCAAACCACTGCCTTCTTTATCCCGAACTTGGCCGGTTCTCGTTCCAACCGTTTCAGGAGAATCCCCTCCTCGGCCCTTCCATCAACACTTTGAAAAGATATGGGGCTTTGGACAAAGAAGCAGTAGTAGAACATGGGGAAGGGTGGCGCTTGGTGTCTTGTATGTGGCTTCATGCTGGAGTTATTCATTTACTTGCAAATATGCTCAGCCTTCTCTTCATAGGAATTCGCCTCGAACAAGAATTTGGATTTTTGAAAATAGGATGTTTATATGTGCTTTCTGGATTTGGTGGGAGTTTACTTTCAGCTCTAAGTATCAACCCAGCTGAAAATCCAACCATATCAGTTGGTGCTTCAGGTGCACTTTTTGGGCTTTTGGGAGCTATGCTTTCTGAGCTCATCACAAATTGGACAATATATGCAAATAAGTTTGCAGCCCTGACGTCTTTGATTCTGATCATTGCCTTAAACCTGGCAGTCGGATTTGTACCACACGTTGACAATTCCGCCCACATCGGAGGCTTCATTTCGGGCTTTCTCCTCGGCTTCATCCTCTTAATCCGACCGCAATTCGGATACGTTAACCACAAGTACATCCCTGCAGGGTCTGATGTGAAACGTAAATCCAAGCACAAATGTTATCAGTACTTTTTGTTGATTATAGCTCTACTGCTCTTGATTTTCGGATATGCTTCTGGTTTAACAAAGCTGTACAGAACTGGTCCACAAGTATTGGAAAGCAACACTTTCTGA

Protein sequence

MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYENNCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASGLTKLYRTGPQVLESNTF
Homology
BLAST of HG10023273 vs. NCBI nr
Match: XP_038899775.1 (RHOMBOID-like protein 5 [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 289/317 (91.17%), Postives = 300/317 (94.64%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKRPPSV  ES SYDVHK +SMPPPPSYYPPPP+PWFPWLVP IF ANVAMF+FTLYEN
Sbjct: 1   MGKRPPSV--ESMSYDVHKPMSMPPPPSYYPPPPKPWFPWLVPLIFIANVAMFIFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPATTG NHCLLYPELGRFSFQPFQENPLLGPS+N L+ YGALDKEAVVE GEGWRLVS
Sbjct: 61  NCPATTGRNHCLLYPELGRFSFQPFQENPLLGPSMNILRIYGALDKEAVVERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           C+WLHAGVIHL+ANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LS+N AE+PT
Sbjct: 121 CIWLHAGVIHLIANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSLNSAESPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANK AAL SLILII LNLAVGF+PHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIGLNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GFISGFLLGFILLIRPQFGYVNHKYIP GSDVKRKSKHKCYQYFLLIIALLLLIFGYASG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNHKYIPVGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LTKLYRTGPQVL SN F
Sbjct: 301 LTKLYRTGPQVLTSNIF 315

BLAST of HG10023273 vs. NCBI nr
Match: XP_023514894.1 (RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 281/317 (88.64%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR P  +IES SY+V K +SMPPPPSYYPPPP+PWFPWLVP  F AN+AMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFAFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPSI TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSITTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LSINP+ENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NK AA  SLILIIALNLAVGF+PH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIPAG+DVKRKSKHKCYQYFLLI+AL+LLIFGYASG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LT+LY+TGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of HG10023273 vs. NCBI nr
Match: XP_022964405.1 (RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025779.1 RHOMBOID-like protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.4 bits (1500), Expect = 2.3e-162
Identity = 281/317 (88.64%), Postives = 299/317 (94.32%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR P  +IES SY+V K +SMPPPPSYYPPPP+PWFPWLVP  F AN+AMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPSI+TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LSINP+ENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NK AA  SLILIIALNLAVGF+PH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIPAG+DVKRKSKHKCYQYFLLI+AL+LLIFGYASG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LT+LY+TGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of HG10023273 vs. NCBI nr
Match: XP_023000373.1 (RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 582.4 bits (1500), Expect = 2.3e-162
Identity = 281/317 (88.64%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR P  +IES SYDV K +SMPPPPSYYPPPP+PWFPWLVP  F AN+AMFVFTLYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPSI+TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LSINP+ENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NK AA  SLILIIALNLAVGF+PH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIPAG+DVKRKSKHKCYQYFLLI+AL+LLIFGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LT+LY+TGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of HG10023273 vs. NCBI nr
Match: XP_038888787.1 (RHOMBOID-like protein 5 [Benincasa hispida])

HSP 1 Score: 575.1 bits (1481), Expect = 3.7e-160
Identity = 282/317 (88.96%), Postives = 299/317 (94.32%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR PSV++ES SYDVHK ISMPPPPSYYPPPP+PWFPWLVP IF ANVAMFVFTLYEN
Sbjct: 1   MGKRLPSVDMESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFIANVAMFVFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPATT  N CLLYPELGRFSFQPFQENPLLGPS+N LKRYGALDK+AVVE GE WRLVS
Sbjct: 61  NCPATTRRNRCLLYPELGRFSFQPFQENPLLGPSMNILKRYGALDKDAVVEQGEAWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           C+WLHAGVIHLLANMLSLLFIG+RLEQEFGFLKIGCLY+LSGFGGSLLS+LS+  AE+PT
Sbjct: 121 CIWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYMLSGFGGSLLSSLSLVSAESPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANK AAL SLILII LNLAVGFVP+VDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALISLILIIGLNLAVGFVPNVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GFISGFLLGF+LLIRPQFGYVNHKYIP G+DVKRKSKHKCYQYFLLIIALLLL+FGYASG
Sbjct: 241 GFISGFLLGFVLLIRPQFGYVNHKYIPPGADVKRKSKHKCYQYFLLIIALLLLVFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           L++LYRTGPQVL SNTF
Sbjct: 301 LSRLYRTGPQVLTSNTF 317

BLAST of HG10023273 vs. ExPASy Swiss-Prot
Match: Q9SSR0 (RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.0e-96
Identity = 183/307 (59.61%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKRPP         D+      P  P + PP P PW  WLVP I  AN   F  T+Y N
Sbjct: 1   MGKRPPI------PPDIENGPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTMYVN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           +CPA   ++ CLL+  LGR SFQP +EN LLGPSI TL++ GAL++  +VE GE WRL+S
Sbjct: 61  DCPAR--SDECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGERWRLIS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           C+WLH G +HL+ANM+SL+ IG+RLEQEFGF++IG LYV+SG GGSL+S L+ +  E   
Sbjct: 121 CIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDSQGER-- 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           +SVGASGALFGLLGAMLSELITNWTIY NK  AL +LILII LNL+VGF+P VDNSAH G
Sbjct: 181 VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSAHFG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVK-RKSKHKCYQYFLLIIALLLLIFGYAS 300
           GF++GF LGF+LL+RPQ+GYVN KYIP G D+K +KSKHKCYQ+     +L +L+ G+ +
Sbjct: 241 GFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAGFIA 296

Query: 301 GLTKLYR 307
           G TKL R
Sbjct: 301 GYTKLLR 296

BLAST of HG10023273 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.0e-91
Identity = 177/275 (64.36%), Postives = 210/275 (76.36%), Query Frame = 0

Query: 33  PPQPWFPWLVPFIFTANVAMFVFTLYENNCPATTGANHCLLYPELGRFSFQPFQENPLLG 92
           P + WFPWLVP I  AN+A+F  +++ NNCP  +   +CL    LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA--YCLA-RFLGRFAFQPMKENPLLG 109

Query: 93  PSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFA 212
           +IG LY++SGFGGSLLS+L         ISVGASGALFGLLGAMLSEL+TNWTIYANKFA
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 ALTSLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAG-SD 272
           AL +LI IIA+NLAVG +PHVDN AH+GGF SGFLLGF+ LIRPQ+GY N +  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
              KSKHK YQY L I +L+LLI GY +GL  L R
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLR 317

BLAST of HG10023273 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.5e-82
Identity = 157/274 (57.30%), Postives = 207/274 (75.55%), Query Frame = 0

Query: 37  WFPWLVPFIFTANVAMFVFTLYENNCPATTGAN----HCLLYPELGRFSFQPFQENPLLG 96
           W  WLVP    ANVA+FV  ++ NNCP    ++    HC+    LGR SF+P + NPL G
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVA-KFLGRLSFEPLRTNPLFG 112

Query: 97  PSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 156
           PS +TL++ GAL+   VVE  EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF+
Sbjct: 113 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 172

Query: 157 KIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFA 216
           +IG +Y+LSG GGS+LS+L I      +ISVGASGALFGLLG+MLSEL TNWTIY+NK A
Sbjct: 173 RIGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIA 232

Query: 217 ALTSLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDV 276
           AL +L+ +I +NLA+G +PHVDN AH+GGF++GFLLGFILL RPQF ++  +++P G+ +
Sbjct: 233 ALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL 292

Query: 277 KRKSKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
             + K+K YQY L +++L+LLI G+   L  L+R
Sbjct: 293 --RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

BLAST of HG10023273 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 151/271 (55.72%), Postives = 203/271 (74.91%), Query Frame = 0

Query: 37  WFPWLVPFIFTANVAMFVFTLYENNCP-ATTGANHCLLYPELGRFSFQPFQENPLLGPSI 96
           W  WL+P I  AN+A+F+  ++ N+CP   TG N   +   LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  NTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIG 156
           +TL++ GAL+   VV   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+++G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFAALT 216
            +Y++SG GGS+LS+L +      +ISVGASGALFGLLGAMLSEL+TNWTIYANK AAL 
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRK 276
           +L+ IIA+NLA+G +P VDN AHIGGF++GF LGF+LL+RPQ+G+   +     +  + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR----TNTSRTK 270

Query: 277 SKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
            K+  YQY L +++++LL+ G    L  L++
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

BLAST of HG10023273 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.9e-80
Identity = 153/269 (56.88%), Postives = 195/269 (72.49%), Query Frame = 0

Query: 37  WFPWLVPFIFTANVAMFVFTLYENNCPATTGANHCLLYPELGRFSFQPFQENPLLGPSIN 96
           WFPWL+P    ANVA+FV T+Y NNCP  +G   C     LGRFSFQ  +ENPLLGPS  
Sbjct: 64  WFPWLIPCFVVANVAVFVITMYVNNCPKKSG--DCFA-DFLGRFSFQNTRENPLLGPSSL 123

Query: 97  TLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGC 156
           TL+  G LD + VV+  EGWRL+SC WLH GV+HLL NML+LLFIGIR+E+EFGF++IG 
Sbjct: 124 TLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIRIGL 183

Query: 157 LYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFAALTS 216
           LY++SGFGGS+LSAL +       ISVGASGA+FGLLG MLSE+  NWTIY+NK   + +
Sbjct: 184 LYLISGFGGSILSALFL----RSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVT 243

Query: 217 LILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKS 276
           L+LI+A+NL +G +P VDN AHIGGF +GFLLGF+LLIRP +G++N +  P      +  
Sbjct: 244 LVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGPGA----KPH 303

Query: 277 KHKCYQYFLLIIALLLLIFGYASGLTKLY 306
           + K YQ  L  I+LL+L+ G+  GL  L+
Sbjct: 304 RFKIYQGILWTISLLILVAGFIVGLISLF 321

BLAST of HG10023273 vs. ExPASy TrEMBL
Match: A0A6J1KMF5 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 281/317 (88.64%), Postives = 298/317 (94.01%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR P  +IES SYDV K +SMPPPPSYYPPPP+PWFPWLVP  F AN+AMFVFTLYEN
Sbjct: 1   MGKRSPPGDIESMSYDVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFVFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPSI+TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LSINP+ENP 
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPI 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NK AA  SLILIIALNLAVGF+PH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIPAG+DVKRKSKHKCYQYFLLI+AL+LLIFGY SG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYVSG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LT+LY+TGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of HG10023273 vs. ExPASy TrEMBL
Match: A0A6J1HKQ1 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 281/317 (88.64%), Postives = 299/317 (94.32%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKR P  +IES SY+V K +SMPPPPSYYPPPP+PWFPWLVP  F AN+AMF FTLYEN
Sbjct: 1   MGKRSPPGDIESMSYEVRKPLSMPPPPSYYPPPPKPWFPWLVPLTFLANIAMFSFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA  G N CL YPELGRFSFQPFQENPLLGPSI+TLKRYGALDKEAVVEHGEGWRLVS
Sbjct: 61  NCPAAIGGNRCLFYPELGRFSFQPFQENPLLGPSISTLKRYGALDKEAVVEHGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLS+LSINP+ENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSSLSINPSENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIY+NK AA  SLILIIALNLAVGF+PH+DNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYSNKCAAFISLILIIALNLAVGFIPHIDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLI+PQFGYVNHKYIPAG+DVKRKSKHKCYQYFLLI+AL+LLIFGYASG
Sbjct: 241 GFVSGFLLGFILLIQPQFGYVNHKYIPAGADVKRKSKHKCYQYFLLIVALILLIFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           LT+LY+TGPQVLESNTF
Sbjct: 301 LTRLYKTGPQVLESNTF 317

BLAST of HG10023273 vs. ExPASy TrEMBL
Match: A0A5A7UZ09 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G002320 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 2.6e-159
Identity = 280/319 (87.77%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAIS-MPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYE 60
           MGKRPPSV++ES SY++HK I+ MPPPPSYYPPPP+PWFPWLVP IF  NV MF+FTLY+
Sbjct: 1   MGKRPPSVDMESMSYNIHKPITIMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFTLYK 60

Query: 61  NNCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLV 120
           NNCPA TG N CLLYPELGRFSFQPF ENPLLGP+I+ L+RYGALDKEAVVE GEGWRLV
Sbjct: 61  NNCPAITGGNRCLLYPELGRFSFQPFHENPLLGPTISILRRYGALDKEAVVERGEGWRLV 120

Query: 121 SCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENP 180
           SC WLHAGVIHLLANMLSLLFIG+RLEQEFGFLKIGCLYVLSGFGGSLLS++S+NPAENP
Sbjct: 121 SCTWLHAGVIHLLANMLSLLFIGVRLEQEFGFLKIGCLYVLSGFGGSLLSSISLNPAENP 180

Query: 181 TISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHI 240
           TISVGASGALFGLLGAMLSELITNWTIYANK AAL SLILIIALNLAVGFVPHVDNSAHI
Sbjct: 181 TISVGASGALFGLLGAMLSELITNWTIYANKCAALMSLILIIALNLAVGFVPHVDNSAHI 240

Query: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYAS 300
           GGFISGFLLGFILLIRPQFGYVNHKYIP GSDVKRKSKHKCYQYFLLI+ALLLL+FGY S
Sbjct: 241 GGFISGFLLGFILLIRPQFGYVNHKYIPPGSDVKRKSKHKCYQYFLLIVALLLLVFGYIS 300

Query: 301 GLTKLYRTGP-QVLESNTF 318
           GLTKLYRTGP QV +SN F
Sbjct: 301 GLTKLYRTGPIQVSKSNRF 319

BLAST of HG10023273 vs. ExPASy TrEMBL
Match: A0A0A0K8T6 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 9.0e-152
Identity = 266/296 (89.86%), Postives = 278/296 (93.92%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKRPPSV++ES SYDVHK ISMPPPPSYYPPPP+PWFPWLVP IF  NV MF+F LYEN
Sbjct: 1   MGKRPPSVHVESMSYDVHKPISMPPPPSYYPPPPKPWFPWLVPLIFLVNVGMFIFMLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPA +G N CLLYPELGRFSFQPF ENPLLGPSI  LKRYGALDKEAV+E GEGWRLVS
Sbjct: 61  NCPAISGRNRCLLYPELGRFSFQPFHENPLLGPSITILKRYGALDKEAVLERGEGWRLVS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL+IGCLYVLSGFGGSLLS++S+NPAENPT
Sbjct: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLRIGCLYVLSGFGGSLLSSISLNPAENPT 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGAMLSELITNWTIYANK AAL SLILIIALNLAVGF+PHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGAMLSELITNWTIYANKCAALLSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFG 297
           GFISGFLLGFILLIRPQFGYVN KYIPAGSDVKRKSKHKCYQY LLIIALLLL+FG
Sbjct: 241 GFISGFLLGFILLIRPQFGYVNQKYIPAGSDVKRKSKHKCYQYLLLIIALLLLVFG 296

BLAST of HG10023273 vs. ExPASy TrEMBL
Match: A0A6J1JR47 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488148 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.5e-151
Identity = 263/317 (82.97%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGK+ P  +IES SY V K +SMPPPPSYYPPPP+PWFP+LVP IF A++A+FVFTLYEN
Sbjct: 1   MGKKLPRDDIESMSYGVRKPLSMPPPPSYYPPPPKPWFPFLVPLIFVADIALFVFTLYEN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           NCPATTG N CL YPEL R+SFQPF ENPLLGPSI TLKRYGALD+EAVVE GEGWRL++
Sbjct: 61  NCPATTGGNRCLFYPELDRYSFQPFSENPLLGPSIMTLKRYGALDREAVVERGEGWRLLT 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           CMWLHAGVIHLLANM+SLLFIG+RLEQEFG+L+IGCLYVLSGFGGSLLS LSINPAE  T
Sbjct: 121 CMWLHAGVIHLLANMISLLFIGVRLEQEFGYLRIGCLYVLSGFGGSLLSCLSINPAET-T 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           ISVGASGALFGLLGA+LSELITNWTIY+NK AA+ SLILIIALNLAVGF+PHVDNSAHIG
Sbjct: 181 ISVGASGALFGLLGALLSELITNWTIYSNKCAAMMSLILIIALNLAVGFIPHVDNSAHIG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRKSKHKCYQYFLLIIALLLLIFGYASG 300
           GF+SGFLLGFILLIRPQFGYVN KYIPAG DVKRKSKH+CYQYFLL++AL+LLIFGYASG
Sbjct: 241 GFLSGFLLGFILLIRPQFGYVNPKYIPAGCDVKRKSKHQCYQYFLLLVALILLIFGYASG 300

Query: 301 LTKLYRTGPQVLESNTF 318
           L++LY+TGPQ+LE+N F
Sbjct: 301 LSRLYKTGPQILEANIF 316

BLAST of HG10023273 vs. TAIR 10
Match: AT1G52580.1 (RHOMBOID-like protein 5 )

HSP 1 Score: 354.8 bits (909), Expect = 7.4e-98
Identity = 183/307 (59.61%), Postives = 226/307 (73.62%), Query Frame = 0

Query: 1   MGKRPPSVNIESKSYDVHKAISMPPPPSYYPPPPQPWFPWLVPFIFTANVAMFVFTLYEN 60
           MGKRPP         D+      P  P + PP P PW  WLVP I  AN   F  T+Y N
Sbjct: 1   MGKRPPI------PPDIENGPPPPARPHFRPPIPVPWVAWLVPLILAANFVTFATTMYVN 60

Query: 61  NCPATTGANHCLLYPELGRFSFQPFQENPLLGPSINTLKRYGALDKEAVVEHGEGWRLVS 120
           +CPA   ++ CLL+  LGR SFQP +EN LLGPSI TL++ GAL++  +VE GE WRL+S
Sbjct: 61  DCPAR--SDECLLFDVLGRLSFQPIKENMLLGPSIPTLRKLGALERR-LVEEGERWRLIS 120

Query: 121 CMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIGCLYVLSGFGGSLLSALSINPAENPT 180
           C+WLH G +HL+ANM+SL+ IG+RLEQEFGF++IG LYV+SG GGSL+S L+ +  E   
Sbjct: 121 CIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVSCLTDSQGER-- 180

Query: 181 ISVGASGALFGLLGAMLSELITNWTIYANKFAALTSLILIIALNLAVGFVPHVDNSAHIG 240
           +SVGASGALFGLLGAMLSELITNWTIY NK  AL +LILII LNL+VGF+P VDNSAH G
Sbjct: 181 VSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVLNLSVGFLPRVDNSAHFG 240

Query: 241 GFISGFLLGFILLIRPQFGYVNHKYIPAGSDVK-RKSKHKCYQYFLLIIALLLLIFGYAS 300
           GF++GF LGF+LL+RPQ+GYVN KYIP G D+K +KSKHKCYQ+     +L +L+ G+ +
Sbjct: 241 GFLAGFFLGFVLLLRPQYGYVNPKYIPPGYDMKHKKSKHKCYQHIFRFTSLAILLAGFIA 296

Query: 301 GLTKLYR 307
           G TKL R
Sbjct: 301 GYTKLLR 296

BLAST of HG10023273 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 335.9 bits (860), Expect = 3.6e-92
Identity = 177/275 (64.36%), Postives = 210/275 (76.36%), Query Frame = 0

Query: 33  PPQPWFPWLVPFIFTANVAMFVFTLYENNCPATTGANHCLLYPELGRFSFQPFQENPLLG 92
           P + WFPWLVP I  AN+A+F  +++ NNCP  +   +CL    LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA--YCLA-RFLGRFAFQPMKENPLLG 109

Query: 93  PSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFA 212
           +IG LY++SGFGGSLLS+L         ISVGASGALFGLLGAMLSEL+TNWTIYANKFA
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFA 229

Query: 213 ALTSLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAG-SD 272
           AL +LI IIA+NLAVG +PHVDN AH+GGF SGFLLGF+ LIRPQ+GY N +  P G + 
Sbjct: 230 ALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAA 289

Query: 273 VKRKSKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
              KSKHK YQY L I +L+LLI GY +GL  L R
Sbjct: 290 PSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLR 317

BLAST of HG10023273 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 315.8 bits (808), Expect = 3.8e-86
Identity = 174/296 (58.78%), Postives = 206/296 (69.59%), Query Frame = 0

Query: 33  PPQPWFPWLVPFIFTANVAMFVFTLYENNCPATTGANHCLLYPELGRFSFQPFQENPLLG 92
           P + WFPWLVP I  AN+A+F  +++ NNCP  +   +CL    LGRF+FQP +ENPLLG
Sbjct: 50  PFKLWFPWLVPAIVVANIALFAISMFINNCPKNSA--YCLA-RFLGRFAFQPMKENPLLG 109

Query: 93  PSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 152
           PS  TL++ GALD   VV   E WRL +C+WLHAGV H+LANMLSL+FIGIRLEQEFGF+
Sbjct: 110 PSSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFV 169

Query: 153 KIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANK-- 212
           +IG LY++SGFGGSLLS+L         ISVGASGALFGLLGAMLSEL+TNWTIYANK  
Sbjct: 170 RIGLLYMISGFGGSLLSSL----FNRAGISVGASGALFGLLGAMLSELLTNWTIYANKVA 229

Query: 213 --------------------------FAALTSLILIIALNLAVGFVPHVDNSAHIGGFIS 272
                                     FAAL +LI IIA+NLAVG +PHVDN AH+GGF S
Sbjct: 230 KSSLVKQAALSMNDVSIMSLVFLHLQFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTS 289

Query: 273 GFLLGFILLIRPQFGYVNHKYIPAG-SDVKRKSKHKCYQYFLLIIALLLLIFGYAS 300
           GFLLGF+ LIRPQ+GY N +  P G +    KSKHK YQY L I +L+LLI GY S
Sbjct: 290 GFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGYTS 338

BLAST of HG10023273 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 307.8 bits (787), Expect = 1.0e-83
Identity = 157/274 (57.30%), Postives = 207/274 (75.55%), Query Frame = 0

Query: 37  WFPWLVPFIFTANVAMFVFTLYENNCPATTGAN----HCLLYPELGRFSFQPFQENPLLG 96
           W  WLVP    ANVA+FV  ++ NNCP    ++    HC+    LGR SF+P + NPL G
Sbjct: 53  WTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVA-KFLGRLSFEPLRTNPLFG 112

Query: 97  PSINTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFL 156
           PS +TL++ GAL+   VVE  EGWRL++C+WLHAGVIHL ANMLSL+FIGIRLEQ+FGF+
Sbjct: 113 PSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFGFV 172

Query: 157 KIGCLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFA 216
           +IG +Y+LSG GGS+LS+L I      +ISVGASGALFGLLG+MLSEL TNWTIY+NK A
Sbjct: 173 RIGVIYLLSGIGGSVLSSLFI----RNSISVGASGALFGLLGSMLSELFTNWTIYSNKIA 232

Query: 217 ALTSLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDV 276
           AL +L+ +I +NLA+G +PHVDN AH+GGF++GFLLGFILL RPQF ++  +++P G+ +
Sbjct: 233 ALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKWLAREHMPQGTPL 292

Query: 277 KRKSKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
             + K+K YQY L +++L+LLI G+   L  L+R
Sbjct: 293 --RYKYKTYQYLLWLLSLVLLIAGFVVALLMLFR 319

BLAST of HG10023273 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 304.7 bits (779), Expect = 8.8e-83
Identity = 151/271 (55.72%), Postives = 203/271 (74.91%), Query Frame = 0

Query: 37  WFPWLVPFIFTANVAMFVFTLYENNCP-ATTGANHCLLYPELGRFSFQPFQENPLLGPSI 96
           W  WL+P I  AN+A+F+  ++ N+CP   TG N   +   LGRFSFQP +ENPL GPS 
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 97  NTLKRYGALDKEAVVEHGEGWRLVSCMWLHAGVIHLLANMLSLLFIGIRLEQEFGFLKIG 156
           +TL++ GAL+   VV   +GWRL+SCMWLHAG+IHLL NMLSL+FIGIRLEQ+FGF+++G
Sbjct: 91  STLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVG 150

Query: 157 CLYVLSGFGGSLLSALSINPAENPTISVGASGALFGLLGAMLSELITNWTIYANKFAALT 216
            +Y++SG GGS+LS+L +      +ISVGASGALFGLLGAMLSEL+TNWTIYANK AAL 
Sbjct: 151 LIYLISGLGGSILSSLFL----QESISVGASGALFGLLGAMLSELLTNWTIYANKAAALI 210

Query: 217 SLILIIALNLAVGFVPHVDNSAHIGGFISGFLLGFILLIRPQFGYVNHKYIPAGSDVKRK 276
           +L+ IIA+NLA+G +P VDN AHIGGF++GF LGF+LL+RPQ+G+   +     +  + K
Sbjct: 211 TLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR----TNTSRTK 270

Query: 277 SKHKCYQYFLLIIALLLLIFGYASGLTKLYR 307
            K+  YQY L +++++LL+ G    L  L++
Sbjct: 271 RKYSMYQYVLFVVSVVLLVVGLTVALVMLFK 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899775.12.1e-16391.17RHOMBOID-like protein 5 [Benincasa hispida][more]
XP_023514894.11.8e-16288.64RHOMBOID-like protein 5 [Cucurbita pepo subsp. pepo][more]
XP_022964405.12.3e-16288.64RHOMBOID-like protein 5 [Cucurbita moschata] >KAG6593439.1 RHOMBOID-like protein... [more]
XP_023000373.12.3e-16288.64RHOMBOID-like protein 5 isoform X1 [Cucurbita maxima][more]
XP_038888787.13.7e-16088.96RHOMBOID-like protein 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9SSR01.0e-9659.61RHOMBOID-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=RBL5 PE=3 SV=1[more]
Q0WQX75.0e-9164.36RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
Q9LYP11.5e-8257.30RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
Q9CAN11.2e-8155.72RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
F4JBM48.9e-8056.88RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KMF51.1e-16288.64RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111494629 PE=3 SV=1[more]
A0A6J1HKQ11.1e-16288.64RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464438 PE=3 SV=1[more]
A0A5A7UZ092.6e-15987.77RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold33... [more]
A0A0A0K8T69.0e-15289.86RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G432610 PE=3 SV=1[more]
A0A6J1JR474.5e-15182.97RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488148 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52580.17.4e-9859.61RHOMBOID-like protein 5 [more]
AT2G29050.13.6e-9264.36RHOMBOID-like 1 [more]
AT2G29050.23.8e-8658.78RHOMBOID-like 1 [more]
AT5G07250.11.0e-8357.30RHOMBOID-like protein 3 [more]
AT1G63120.18.8e-8355.72RHOMBOID-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 38..255
e-value: 1.9E-38
score: 134.0
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 36..257
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 111..255
e-value: 1.9E-41
score: 141.5
NoneNo IPR availablePANTHERPTHR22936:SF87RHOMBOID-LIKE PROTEIN 5coord: 1..306
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 1..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023273.1HG10023273.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity