HG10023227 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023227 vs. NCBI nr
Match: XP_038897019.1 (uncharacterized protein At2g39795, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 449.1 bits (1154), Expect = 2.4e-122
Identity = 226/257 (87.94%), Postives = 231/257 (89.88%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSSS-------------SHKALISQSHLQNHITFPPENPYVFFKTLR 60
           MARLIRP RKCLQ SSS             SHKALISQS+LQN ITFPPENPYVFFKT R
Sbjct: 1   MARLIRPWRKCLQFSSSSSSSSSSYYYYSYSHKALISQSNLQNPITFPPENPYVFFKT-R 60

Query: 61  HYISDMRRSAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYG 120
            YISDMRRSAFEGNILRLLRNEIRYE DRSPP QPVT FGAFT+DGRPGEQWIRLEREYG
Sbjct: 61  PYISDMRRSAFEGNILRLLRNEIRYELDRSPPYQPVTKFGAFTVDGRPGEQWIRLEREYG 120

Query: 121 ENENIKIEVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSL 180
           ENENIKIEVTMFD SIP+P SGG+SSGEDV+LHITTIVNITKGGGSEVLEI CSAWPDSL
Sbjct: 121 ENENIKIEVTMFDRSIPAPNSGGNSSGEDVILHITTIVNITKGGGSEVLEIMCSAWPDSL 180

Query: 181 EIDRLFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDK 240
           EIDRLFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLE RGVDDQLAVFLHKYMKNKDK
Sbjct: 181 EIDRLFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEVRGVDDQLAVFLHKYMKNKDK 240

Query: 241 TEFIRWMETVKAFIEIN 245
           TEFIRWMETVKAFIE N
Sbjct: 241 TEFIRWMETVKAFIETN 256

BLAST of HG10023227 vs. NCBI nr
Match: XP_004135870.1 (uncharacterized protein At2g39795, mitochondrial [Cucumis sativus] >KGN45225.1 hypothetical protein Csa_016252 [Cucumis sativus])

HSP 1 Score: 429.5 bits (1103), Expect = 2.0e-116
Identity = 210/248 (84.68%), Postives = 226/248 (91.13%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSS-----SSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRR 60
           MARLIRPLRKCLQ SS     SSHK+L +Q HLQN I FPPEN YVF KTLRHYI+DMRR
Sbjct: 1   MARLIRPLRKCLQFSSSSSSFSSHKSLTTQLHLQNPIVFPPENHYVFCKTLRHYIADMRR 60

Query: 61  SAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIE 120
           SAFEGNILRLLRNEIRYE DRSPP+QPVT FGAFT+D RPGEQWIRLEREY E E IKIE
Sbjct: 61  SAFEGNILRLLRNEIRYELDRSPPSQPVTKFGAFTVDERPGEQWIRLEREYEEREKIKIE 120

Query: 121 VTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLR 180
            TMFD SIP+PKSGGSSSG+DVLLHITTIV+ITKGGGS+VL+I CSAWPDS+EIDRLFLR
Sbjct: 121 ATMFDQSIPAPKSGGSSSGKDVLLHITTIVHITKGGGSDVLKIICSAWPDSIEIDRLFLR 180

Query: 181 RGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWME 240
            GE+MPAQPYAGP+FKELDDDLQNSLYEYLEARGVDDQLAVFLH+YMKNKDKTEFIRWME
Sbjct: 181 GGEIMPAQPYAGPDFKELDDDLQNSLYEYLEARGVDDQLAVFLHEYMKNKDKTEFIRWME 240

Query: 241 TVKAFIEI 244
           T+KAFIE+
Sbjct: 241 TIKAFIEM 248

BLAST of HG10023227 vs. NCBI nr
Match: XP_038897018.1 (mitochondrial acidic protein mam33 isoform X1 [Benincasa hispida])

HSP 1 Score: 423.3 bits (1087), Expect = 1.4e-114
Identity = 226/313 (72.20%), Postives = 231/313 (73.80%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSSS-------------SHKALISQSHLQNHITFPPENPYVFFKTLR 60
           MARLIRP RKCLQ SSS             SHKALISQS+LQN ITFPPENPYVFFKT R
Sbjct: 1   MARLIRPWRKCLQFSSSSSSSSSSYYYYSYSHKALISQSNLQNPITFPPENPYVFFKT-R 60

Query: 61  HYISDMRRSAFEGNILRLLRNEIRYEFDRSPPNQ-------------------------- 120
            YISDMRRSAFEGNILRLLRNEIRYE DRSPP Q                          
Sbjct: 61  PYISDMRRSAFEGNILRLLRNEIRYELDRSPPYQHAKIFTNDYKIEHRCVLVGCSTGVFE 120

Query: 121 ------------------------------PVTNFGAFTIDGRPGEQWIRLEREYGENEN 180
                                         PVT FGAFT+DGRPGEQWIRLEREYGENEN
Sbjct: 121 GNCYFVEDEFGLNNLHHRACLELLTFLGMEPVTKFGAFTVDGRPGEQWIRLEREYGENEN 180

Query: 181 IKIEVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDR 240
           IKIEVTMFD SIP+P SGG+SSGEDV+LHITTIVNITKGGGSEVLEI CSAWPDSLEIDR
Sbjct: 181 IKIEVTMFDRSIPAPNSGGNSSGEDVILHITTIVNITKGGGSEVLEIMCSAWPDSLEIDR 240

Query: 241 LFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFI 245
           LFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLE RGVDDQLAVFLHKYMKNKDKTEFI
Sbjct: 241 LFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEVRGVDDQLAVFLHKYMKNKDKTEFI 300

BLAST of HG10023227 vs. NCBI nr
Match: XP_008461172.1 (PREDICTED: uncharacterized protein At2g39795, mitochondrial [Cucumis melo])

HSP 1 Score: 412.9 bits (1060), Expect = 1.9e-111
Identity = 206/249 (82.73%), Postives = 220/249 (88.35%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSS------SSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMR 60
           MARLIRPLR+CLQ SS       SHK+LI+Q HLQN I F  EN YVF KT+RHYI+DMR
Sbjct: 1   MARLIRPLRECLQFSSYFSSSFPSHKSLITQLHLQNPIVFLHENHYVFRKTVRHYIADMR 60

Query: 61  RSAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKI 120
           RSAFEGNILRLLRNEIRYE DRSPP+QPVT FGAFT+D RPGEQWIRLEREYG+ E IKI
Sbjct: 61  RSAFEGNILRLLRNEIRYELDRSPPSQPVTKFGAFTVDERPGEQWIRLEREYGDKEKIKI 120

Query: 121 EVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFL 180
           E TMFD SIP+ KSGGSSS EDVLLHIT IV+ITK GGS+VL I CSAWPDSLEIDRLFL
Sbjct: 121 EATMFDQSIPASKSGGSSSSEDVLLHITAIVHITKVGGSDVLRIMCSAWPDSLEIDRLFL 180

Query: 181 RRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWM 240
           RRGE+MPAQPYAGPEFKELDDDLQNSLYEYLEAR VDDQLAVFLH+YMKNKDKTEFIRWM
Sbjct: 181 RRGEIMPAQPYAGPEFKELDDDLQNSLYEYLEAREVDDQLAVFLHEYMKNKDKTEFIRWM 240

Query: 241 ETVKAFIEI 244
           ETVKAFIE+
Sbjct: 241 ETVKAFIEM 249

BLAST of HG10023227 vs. NCBI nr
Match: KAG7025716.1 (putative protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 405.6 bits (1041), Expect = 3.0e-109
Identity = 204/245 (83.27%), Postives = 214/245 (87.35%), Query Frame = 0

Query: 1   MARLIRPLRKCLQL-SSSSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRRSAFE 60
           MAR+IRPLRKCLQ  SSSSHKAL+SQ+H  N I FPPENP V FKTLRHYIS+MRRSAFE
Sbjct: 31  MARVIRPLRKCLQFSSSSSHKALMSQAHRLNPIPFPPENPCVLFKTLRHYISEMRRSAFE 90

Query: 61  GNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIEVTMF 120
           G+ILRLLRNEIRYE +R PP+QPVT FGAFTID RPGEQWIRLEREYGENENIKIEVTMF
Sbjct: 91  GSILRLLRNEIRYELERHPPDQPVTKFGAFTIDERPGEQWIRLEREYGENENIKIEVTMF 150

Query: 121 DHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLRRGEM 180
           DH+   P       GEDVLLHIT IVNITK   SEVLEI CSAWP+ LEIDRL  RRGE 
Sbjct: 151 DHARTPP-------GEDVLLHITIIVNITKRDSSEVLEIMCSAWPNDLEIDRLLTRRGEK 210

Query: 181 MPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA 240
           MPAQPYAGPEFKELDDDLQNSLY+YLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA
Sbjct: 211 MPAQPYAGPEFKELDDDLQNSLYDYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA 268

Query: 241 FIEIN 245
           FIEIN
Sbjct: 271 FIEIN 268

BLAST of HG10023227 vs. ExPASy Swiss-Prot
Match: Q8W487 (Uncharacterized protein At2g39795, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g39795 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.9e-19
Identity = 65/213 (30.52%), Postives = 110/213 (51.64%), Query Frame = 0

Query: 36  PENPYVFFKTLRHYISDMRRSAFEGNILRLLRNEIR--YEFDRSPPNQPVT-NFGAFTID 95
           P +   F     +Y + + R + E  ++R++ +EI    + D    ++ +T     F I+
Sbjct: 39  PSSIRPFVSRALNYSTAIDRISSEQTLIRVIDSEINSALQSDNIDSDEEMTPGSFPFRIE 98

Query: 96  GRPGEQWIRLEREYGENENIKIEVTMFD-HSIPSPKSGGSSSGEDVLLHITTIVNITKGG 155
            +PG Q + L R+Y   E+IK+ V+M    S  +        G      I  +V +TK  
Sbjct: 99  DKPGNQNVTLTRDY-NGEHIKVVVSMPSLVSDENDDDDDDDEGPSNESSIPLVVTVTKKS 158

Query: 156 GSEVLEITCSAWPDSLEIDRLFLRR-GEMMPAQ-PYAGPEFKELDDDLQNSLYEYLEARG 215
           G   LE +C A+PD + ID L ++  G+ +  Q    GP+F++LD++L+ + Y++LE RG
Sbjct: 159 GL-TLEFSCMAFPDEIAIDALSVKHPGDSLEDQLANEGPDFEDLDENLKKTFYKFLEIRG 218

Query: 216 VDDQLAVFLHKYMKNKDKTEFIRWMETVKAFIE 243
           V      FLH+YM  K   E+  W++ VK F+E
Sbjct: 219 VKASTTNFLHEYMTRKVNREYFLWLKNVKEFME 249

BLAST of HG10023227 vs. ExPASy Swiss-Prot
Match: O22288 (Uncharacterized protein At2g39790, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g39790 PE=3 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 7.5e-10
Identity = 44/152 (28.95%), Postives = 75/152 (49.34%), Query Frame = 0

Query: 91  IDGRPGEQWIRLEREYGENENIKIEVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKG 150
           I  +PG+Q + L   Y + E I ++V M           G ++ E   L    +V + K 
Sbjct: 95  IRDKPGDQSVTLTAYYND-ERIHVDVGMPYLGDDVIDVFGPNNDE---LSFPLVVTVIKK 154

Query: 151 GGSEVLEITCSAWPDSLEIDRLFLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGV 210
            G  + E TC A+ D +++  L +   +    +    P FK LDD+L+ + + YL  R +
Sbjct: 155 NGVSI-EFTCQAYADYIDLTDLTVHDYQYQMGET-DWPRFKNLDDNLKKAFHRYLATR-L 214

Query: 211 DDQLAVFLHKYMKNKDKTEFIRWMETVKAFIE 243
           D  +   LHKYM +K K E++ W++ V  F++
Sbjct: 215 DANITKLLHKYMVSKIKREYLLWLKNVNKFVD 239

BLAST of HG10023227 vs. ExPASy Swiss-Prot
Match: O94675 (Mitochondrial acidic protein mam33 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC776.07 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 35/110 (31.82%), Postives = 59/110 (53.64%), Query Frame = 0

Query: 145 VNITK-GGGSEVLEITCSAWPDSLEIDRLFLRR-----------GEMMPAQPYAGPEFKE 204
           + I+K G G+ V E T  A  D  +I+ ++  +            E    + Y GP FKE
Sbjct: 162 IEISKPGNGALVFEAT--ALDDGFDIENIYFSKDIDMLTSDSLEAEWKRRKQYLGPSFKE 221

Query: 205 LDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKAFIE 243
           LD +LQ+  + YLE R +D+ L+ F+  +   K+  E+I W+E+V+ F++
Sbjct: 222 LDPELQDLFHSYLEERKIDESLSSFIVSFGLTKELKEYINWLESVRQFLK 269

BLAST of HG10023227 vs. ExPASy Swiss-Prot
Match: P40513 (Mitochondrial acidic protein MAM33 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAM33 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.2e-09
Identity = 27/56 (48.21%), Postives = 39/56 (69.64%), Query Frame = 0

Query: 185 YAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKAF 241
           Y GP F  LD++LQ SL  YLE+RGV+++LA F+  Y + K+  E+I W+E +K F
Sbjct: 209 YHGPPFSNLDEELQESLEAYLESRGVNEELASFISAYSEFKENNEYISWLEKMKKF 264

BLAST of HG10023227 vs. ExPASy TrEMBL
Match: A0A0A0K687 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432060 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 9.5e-117
Identity = 210/248 (84.68%), Postives = 226/248 (91.13%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSS-----SSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRR 60
           MARLIRPLRKCLQ SS     SSHK+L +Q HLQN I FPPEN YVF KTLRHYI+DMRR
Sbjct: 1   MARLIRPLRKCLQFSSSSSSFSSHKSLTTQLHLQNPIVFPPENHYVFCKTLRHYIADMRR 60

Query: 61  SAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIE 120
           SAFEGNILRLLRNEIRYE DRSPP+QPVT FGAFT+D RPGEQWIRLEREY E E IKIE
Sbjct: 61  SAFEGNILRLLRNEIRYELDRSPPSQPVTKFGAFTVDERPGEQWIRLEREYEEREKIKIE 120

Query: 121 VTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLR 180
            TMFD SIP+PKSGGSSSG+DVLLHITTIV+ITKGGGS+VL+I CSAWPDS+EIDRLFLR
Sbjct: 121 ATMFDQSIPAPKSGGSSSGKDVLLHITTIVHITKGGGSDVLKIICSAWPDSIEIDRLFLR 180

Query: 181 RGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWME 240
            GE+MPAQPYAGP+FKELDDDLQNSLYEYLEARGVDDQLAVFLH+YMKNKDKTEFIRWME
Sbjct: 181 GGEIMPAQPYAGPDFKELDDDLQNSLYEYLEARGVDDQLAVFLHEYMKNKDKTEFIRWME 240

Query: 241 TVKAFIEI 244
           T+KAFIE+
Sbjct: 241 TIKAFIEM 248

BLAST of HG10023227 vs. ExPASy TrEMBL
Match: A0A1S3CFB4 (uncharacterized protein At2g39795, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103499834 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.2e-112
Identity = 206/249 (82.73%), Postives = 220/249 (88.35%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSS------SSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMR 60
           MARLIRPLR+CLQ SS       SHK+LI+Q HLQN I F  EN YVF KT+RHYI+DMR
Sbjct: 1   MARLIRPLRECLQFSSYFSSSFPSHKSLITQLHLQNPIVFLHENHYVFRKTVRHYIADMR 60

Query: 61  RSAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKI 120
           RSAFEGNILRLLRNEIRYE DRSPP+QPVT FGAFT+D RPGEQWIRLEREYG+ E IKI
Sbjct: 61  RSAFEGNILRLLRNEIRYELDRSPPSQPVTKFGAFTVDERPGEQWIRLEREYGDKEKIKI 120

Query: 121 EVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFL 180
           E TMFD SIP+ KSGGSSS EDVLLHIT IV+ITK GGS+VL I CSAWPDSLEIDRLFL
Sbjct: 121 EATMFDQSIPASKSGGSSSSEDVLLHITAIVHITKVGGSDVLRIMCSAWPDSLEIDRLFL 180

Query: 181 RRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWM 240
           RRGE+MPAQPYAGPEFKELDDDLQNSLYEYLEAR VDDQLAVFLH+YMKNKDKTEFIRWM
Sbjct: 181 RRGEIMPAQPYAGPEFKELDDDLQNSLYEYLEAREVDDQLAVFLHEYMKNKDKTEFIRWM 240

Query: 241 ETVKAFIEI 244
           ETVKAFIE+
Sbjct: 241 ETVKAFIEM 249

BLAST of HG10023227 vs. ExPASy TrEMBL
Match: A0A6J1CTD1 (uncharacterized protein At2g39795, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111014400 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.3e-109
Identity = 206/249 (82.73%), Postives = 216/249 (86.75%), Query Frame = 0

Query: 1   MARLIRPLRKCLQL----SSSSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRRS 60
           MAR+IR LRK LQ     SSSSHK LISQ   QN I F PENP V+  TLRHYISDMRRS
Sbjct: 1   MARVIRSLRKSLQFSSYSSSSSHKTLISQVRRQNLIAFRPENPCVYSNTLRHYISDMRRS 60

Query: 61  AFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIEV 120
           AFEGNILRLLRNEIRYE +RSPPNQ VT FGAFT+D R GEQWIRLER+Y ENENIKIEV
Sbjct: 61  AFEGNILRLLRNEIRYELERSPPNQAVTKFGAFTVDERLGEQWIRLERKYAENENIKIEV 120

Query: 121 TMFDHSIPSPKSGGSS-SGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLR 180
           TMFD SIP+PKSGGSS + EDVLLHIT IVNITKG  S+VLEI CSAWPDSLEIDR FLR
Sbjct: 121 TMFDRSIPAPKSGGSSGTVEDVLLHITVIVNITKGDSSDVLEIMCSAWPDSLEIDRFFLR 180

Query: 181 RGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWME 240
           RGE MPAQPYAGPEFKELDDDLQNSLYEYLEARGVDD+LAVFLHKYM NKDKTEFIRWME
Sbjct: 181 RGEKMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDELAVFLHKYMNNKDKTEFIRWME 240

Query: 241 TVKAFIEIN 245
           TVK+FIEIN
Sbjct: 241 TVKSFIEIN 249

BLAST of HG10023227 vs. ExPASy TrEMBL
Match: A0A6J1H8H8 (uncharacterized protein At2g39795, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111460618 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 9.5e-109
Identity = 202/245 (82.45%), Postives = 213/245 (86.94%), Query Frame = 0

Query: 1   MARLIRPLRKCLQL-SSSSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRRSAFE 60
           MAR+IRPLRKCLQ  SSSSHKAL+SQ+H  N I FPPENP V FKTLRHYIS+MRRSAFE
Sbjct: 1   MARVIRPLRKCLQFSSSSSHKALMSQAHRLNPIPFPPENPCVLFKTLRHYISEMRRSAFE 60

Query: 61  GNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIEVTMF 120
           G+ILRLLRNEIRYE +R PP+QPVT FGAFTID RPGEQWIRLEREYGENENIKIEVTMF
Sbjct: 61  GSILRLLRNEIRYELERHPPDQPVTKFGAFTIDERPGEQWIRLEREYGENENIKIEVTMF 120

Query: 121 DHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLRRGEM 180
           DH+   P       GEDVLLHIT IVNITK   SEVLEI CSAWP+ LEIDRL  RRGE 
Sbjct: 121 DHARTPP-------GEDVLLHITIIVNITKRDSSEVLEIMCSAWPNDLEIDRLLTRRGEK 180

Query: 181 MPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA 240
           MPAQPYAGP FKELDDDLQNSLY+YLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA
Sbjct: 181 MPAQPYAGPAFKELDDDLQNSLYDYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKA 238

Query: 241 FIEIN 245
           FIE+N
Sbjct: 241 FIEVN 238

BLAST of HG10023227 vs. ExPASy TrEMBL
Match: A0A6J1KT90 (uncharacterized protein At2g39795, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111498020 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.8e-108
Identity = 202/247 (81.78%), Postives = 213/247 (86.23%), Query Frame = 0

Query: 1   MARLIRPLRKCLQL---SSSSHKALISQSHLQNHITFPPENPYVFFKTLRHYISDMRRSA 60
           MAR+IRPLRKCLQ    SSSSHKAL+SQ+H  N I FPPENP V FKTLRHYIS+MRRSA
Sbjct: 1   MARVIRPLRKCLQFSSSSSSSHKALMSQAHRLNPIPFPPENPRVLFKTLRHYISEMRRSA 60

Query: 61  FEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIEVT 120
           FEG+ILRLLRNEIRYE +R PP+QPVT FGAFTID RPGEQWIRLEREYGENENIKIEVT
Sbjct: 61  FEGSILRLLRNEIRYELERHPPDQPVTKFGAFTIDERPGEQWIRLEREYGENENIKIEVT 120

Query: 121 MFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLRRG 180
           MFDH+   P       GEDVLLHIT IVNI K   SEVLEI CSAWP+ LEIDRL  RRG
Sbjct: 121 MFDHARTPP-------GEDVLLHITIIVNINKRDSSEVLEIMCSAWPNDLEIDRLLTRRG 180

Query: 181 EMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETV 240
           E MPAQPYAGPEFKELDDDLQNSLY+YLEARGVDDQLAVFLHKY+KNKDKTEFIRWMETV
Sbjct: 181 EKMPAQPYAGPEFKELDDDLQNSLYDYLEARGVDDQLAVFLHKYVKNKDKTEFIRWMETV 240

Query: 241 KAFIEIN 245
           KAFIEIN
Sbjct: 241 KAFIEIN 240

BLAST of HG10023227 vs. TAIR 10
Match: AT1G15870.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 258.5 bits (659), Expect = 5.6e-69
Identity = 134/247 (54.25%), Postives = 173/247 (70.04%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSSSSHKALISQSHLQNHITFPPENPYVF-----FKTLRHYISDMRR 60
           MARL+R LR+    SSS+     S ++    I       Y+F     F +   Y+S+MRR
Sbjct: 1   MARLLRCLRRSFIFSSST-----SIAYRNPRICVQSNQSYIFLSSRKFLSSGSYVSEMRR 60

Query: 61  SAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIE 120
           +AFEGNILRL+R EI+ E D SP  QP  +FG FT+D RPGEQWI L R++GE E+IKIE
Sbjct: 61  TAFEGNILRLIRLEIQSELDHSPILQPEESFGPFTVDERPGEQWISLRRKFGEKEDIKIE 120

Query: 121 VTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLR 180
            TMFD S+PS KS  +S  EDV LHIT IVNI+KG G E LEI CSAWPD+++I + F+R
Sbjct: 121 ATMFDGSVPSSKS-TTSDPEDVQLHITFIVNISKGDG-ETLEIMCSAWPDTIQITKFFVR 180

Query: 181 RGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWME 240
           +        Y GPEF+E++D+LQ+S+Y +LE RG+ D LA FLH+YMKNKDK E+IRWME
Sbjct: 181 KSSQNSPNAYIGPEFQEMEDELQDSVYRFLEERGISDDLAEFLHQYMKNKDKAEYIRWME 240

Query: 241 TVKAFIE 243
           TVK+++E
Sbjct: 241 TVKSYVE 240

BLAST of HG10023227 vs. TAIR 10
Match: AT1G80720.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.3e-67
Identity = 114/190 (60.00%), Postives = 150/190 (78.95%), Query Frame = 0

Query: 53  MRRSAFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENI 112
           M++SAF+GNILRL+RNEI YE D SPP QP  +FG FT+D RPGEQWI L+R +G+ E+I
Sbjct: 1   MQKSAFQGNILRLIRNEIEYELDHSPPLQPPNSFGPFTVDERPGEQWISLKRNFGDKEDI 60

Query: 113 KIEVTMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRL 172
           KIE TMFD S+P+ KS  + +  + +LHIT IVNI+K G +E LEI CSAWPD++EI +L
Sbjct: 61  KIEATMFDRSVPTSKS--TKTEPEYILHITFIVNISKAGATEALEIMCSAWPDTIEISKL 120

Query: 173 FLRRGEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIR 232
            +RRG       Y GPEF+ELDD LQ++LY++LE RG+ D+LAVFLH+YMKNK K E++R
Sbjct: 121 CIRRGINTSPSSYGGPEFEELDDQLQDALYQFLEERGISDELAVFLHRYMKNKGKAEYVR 180

Query: 233 WMETVKAFIE 243
           WME+VK+++E
Sbjct: 181 WMESVKSYVE 188

BLAST of HG10023227 vs. TAIR 10
Match: AT4G31930.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 196.1 bits (497), Expect = 3.4e-50
Identity = 102/245 (41.63%), Postives = 150/245 (61.22%), Query Frame = 0

Query: 1   MARLIRPLRKCLQLSSSSHKALIS----QSHLQNHITFPPENPYVFFKTLRHYISDMRRS 60
           MA L+R L+K     S S + LIS    +S L NH +             R   +   +S
Sbjct: 1   MALLLRTLQKSRISPSHSSRTLISWVRCKSLLPNHQS-------------RDVTTSPAKS 60

Query: 61  AFEGNILRLLRNEIRYEFDRSPPNQPVTNFGAFTIDGRPGEQWIRLEREYGENENIKIEV 120
            F  NILR++RNEI Y+ D +PP+QP T F +F+++  PGEQ I ++ ++GE+E+IK+E 
Sbjct: 61  PFRSNILRIIRNEIEYQSDYAPPHQPATEFKSFSVEDCPGEQCIVMKGKFGEDEDIKMEA 120

Query: 121 TMFDHSIPSPKSGGSSSGEDVLLHITTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLRR 180
           TMFD  +  P++G  +SG DV LHI+ +V+I+K  GSE +E  CS WP+ +EI +L+  R
Sbjct: 121 TMFDGFMNVPRAGLDASGHDVRLHISLLVDISKVDGSEEIEFLCSVWPNRIEIRKLYKLR 180

Query: 181 GEMMPAQPYAGPEFKELDDDLQNSLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMET 240
              +  QPY GP F  L  D Q ++ E+L  RG+D +L  FLH+YM NKD+ E I+W+  
Sbjct: 181 RNKITGQPYMGPNFGNLKYDFQTAIREFLRVRGIDAELCFFLHEYMMNKDRIELIQWLRK 232

Query: 241 VKAFI 242
           + +FI
Sbjct: 241 LNSFI 232

BLAST of HG10023227 vs. TAIR 10
Match: AT3G55605.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.1e-20
Identity = 52/163 (31.90%), Postives = 89/163 (54.60%), Query Frame = 0

Query: 89  FTIDGRPGEQWIRLEREYGENENIKIEVTM-------FDHSIPSPKSGGSSSGEDVLLHI 148
           F I+  PG + + L REY   E IK+EV+M        +  +   + G     +     I
Sbjct: 97  FKIEDNPGHRTVTLTREY-NGEQIKVEVSMPGLAMDENEDDVDDDEDGDGRHEKSNESSI 156

Query: 149 TTIVNITKGGGSEVLEITCSAWPDSLEIDRLFLRRGEMMPAQ--PYAGPEFKELDDDLQN 208
             +V +TK  G   LE +C+A+PD + ID L + R +    +   Y GP+F+ELD++++ 
Sbjct: 157 PLVVTVTKKSGLS-LEFSCTAFPDEIVIDGLSVNRPDDSSEEQLTYDGPDFQELDENMRK 216

Query: 209 SLYEYLEARGVDDQLAVFLHKYMKNKDKTEFIRWMETVKAFIE 243
           S +++LE RG+      FL++YM  KD  E++ W++ +K F++
Sbjct: 217 SFHKFLETRGIKASATDFLYEYMMKKDSREYLLWLKKLKTFVQ 257

BLAST of HG10023227 vs. TAIR 10
Match: AT2G39795.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 96.7 bits (239), Expect = 2.8e-20
Identity = 65/213 (30.52%), Postives = 110/213 (51.64%), Query Frame = 0

Query: 36  PENPYVFFKTLRHYISDMRRSAFEGNILRLLRNEIR--YEFDRSPPNQPVT-NFGAFTID 95
           P +   F     +Y + + R + E  ++R++ +EI    + D    ++ +T     F I+
Sbjct: 39  PSSIRPFVSRALNYSTAIDRISSEQTLIRVIDSEINSALQSDNIDSDEEMTPGSFPFRIE 98

Query: 96  GRPGEQWIRLEREYGENENIKIEVTMFD-HSIPSPKSGGSSSGEDVLLHITTIVNITKGG 155
            +PG Q + L R+Y   E+IK+ V+M    S  +        G      I  +V +TK  
Sbjct: 99  DKPGNQNVTLTRDY-NGEHIKVVVSMPSLVSDENDDDDDDDEGPSNESSIPLVVTVTKKS 158

Query: 156 GSEVLEITCSAWPDSLEIDRLFLRR-GEMMPAQ-PYAGPEFKELDDDLQNSLYEYLEARG 215
           G   LE +C A+PD + ID L ++  G+ +  Q    GP+F++LD++L+ + Y++LE RG
Sbjct: 159 GL-TLEFSCMAFPDEIAIDALSVKHPGDSLEDQLANEGPDFEDLDENLKKTFYKFLEIRG 218

Query: 216 VDDQLAVFLHKYMKNKDKTEFIRWMETVKAFIE 243
           V      FLH+YM  K   E+  W++ VK F+E
Sbjct: 219 VKASTTNFLHEYMTRKVNREYFLWLKNVKEFME 249