HG10023194 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023194
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmitogen-activated protein kinase kinase kinase 2-like
LocationChr05: 32058430 .. 32059737 (+)
RNA-Seq ExpressionHG10023194
SyntenyHG10023194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTCCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTAACTAATCGATTAAATTACTCTACAATTTAACAAAGAATATTATAATCTCAAATCTTATATCTTAATTAATCATCCAATTTAATTTCTTACTTAATTTTCTCTTTGCCCTAAACCTAGGGTTATACTAAACTTTTGCCTCTTCTTTCAAATTTAGTTGGTTAATTATATTAATTTATCTAACCTTAACATTGGTAATTTCATACTAAGTATTATTGATTTCCACTTACTAACATAATAGTGTAAAATTTTGGTGGTTTTCTTCGAAAATTTAATTTATTGGTTGCTCTATCAATGCAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAACCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

mRNA sequence

ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTCCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAACCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

Coding sequence (CDS)

ATGAAGAGAAATTCAGTGGAAGAAGCCGAAGCAGAAGAAACCCCATTAATCACTTCTTCTTCTTCTTTCAACAATGGAATCCAATGGGAACGAGGGCGGCTCATTGGAAAAGGAAGCTTTGGTTCGGTTTTCTTGGCTTCTCGCAAACCACACATCTCTAAATACACTATTTTCCCTCCGATAATGGCTGTTAAATCCGCTGAAATTTCCGTTTCCGAAACCCTACAAAAGGAGAAACAAAATTACGACAACTTGAAAGGATGCAATTCATTAATCCAATGCTTCGCCGAAGAGATTACTACTGACCATAACGGTCACATGATCTATAATTTGCTGCTTGAGGTCGCCGCTGGAGGAACCTTAGCTCATCATATCAACAACACAGGTGGAAAGGGTTTACCAGAAAATGAAGTTCGGAGTTACACGAAATCAATAATCAAAGGATTAATTCACATTCACAAAAACCAATACGTTCACTGTGATTTGAAGCCTGCGAATATTTTATTGGTGCCGAGGAATACTACAAAGGGCCGCCGATTCGTGGCAAAGATCGCCGATCTAGGGTTGGCGAGGAGAACCAGCAAGACAAAGCCAAGCTATTGTTTGGGAGGGACATTGTCCTACATGGCGCCTGAGACATTGATTGACAGCGTGCAAGAATCGTCGAGTGATATTTGGGCACTCGGCTGCCTCGTCCTCCAAATGCTCACCGGAAACCTCCCGTGGACTGCCTCGGACAAGGCTGGGATTGTGAAACAGATCACGGAGGATTATGGAATGCCAAAGATTCCAGAAGGGTTGTCAGCGGAGGCAAGTCGGTTTTTGAAGAATTGCTTTGTGAGGAATCCGACGTTCAGGTTCACGGCGGAGATGCTTATGATAGTGCCGTTTGTGGCGGGGGTTGAAGATCAGGAAGAAGATGAAGATTTTGTGAGAGCTCCATCATTTGTGAAGAAGAGGCCTAAACAGTTTAAGAGGCAAAGAATAATTCCAATTAAAGCTGTGTAA

Protein sequence

MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV
Homology
BLAST of HG10023194 vs. NCBI nr
Match: XP_004136102.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus])

HSP 1 Score: 530.4 bits (1365), Expect = 1.1e-146
Identity = 266/337 (78.93%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR S+ E EA +      SS FNNGIQW++GRLIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIH+H++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIVK++TE++ GMPKIPEGLS EA+ FLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V+AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

BLAST of HG10023194 vs. NCBI nr
Match: XP_008461430.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-146
Identity = 265/337 (78.64%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN + E EA +      S+ FNNGIQW+RGRLIGKGSFGSVFLA  KPH +KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQVE--EGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GI++++TE++ GMPKIPEGLSAEA+ FLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V+AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

BLAST of HG10023194 vs. NCBI nr
Match: KAA0058688.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa] >TYK10494.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 528.9 bits (1361), Expect = 3.3e-146
Identity = 266/337 (78.93%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN + E EA +      SS FNNGIQW+RGRLIGKGSFGSVFLA  KPH  KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQVE--EGSSEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNG M YNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIV+++TE++ GMPKIPEGLSAEA+ FLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGIVQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V+AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

BLAST of HG10023194 vs. NCBI nr
Match: KAE8646420.1 (hypothetical protein Csa_015819 [Cucumis sativus])

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-145
Identity = 261/328 (79.57%), Postives = 288/328 (87.80%), Query Frame = 0

Query: 10  EAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEI 69
           E E   +   SS FNNGIQW++GRLIGKGSFGSVFLAS KPH +KY+IFPP+MAVKSAEI
Sbjct: 3   EKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVMAVKSAEI 62

Query: 70  SVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTG 129
           SVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGTLAHHI NTG
Sbjct: 63  SVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLAHHIKNTG 122

Query: 130 GKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRRFVAKIADLG 189
           GKGL EN VR+YTKSIIKGLIH+H++QYVHCDLKPANILL+P+ NTTK R+F+AKIADLG
Sbjct: 123 GKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLG 182

Query: 190 LARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA 249
           LARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN  W A+DK 
Sbjct: 183 LARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRAWAATDKV 242

Query: 250 GIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEED 309
           GIVK++TE++ GMPKIPEGLS EA+ FLKNC VR P FRFTAEMLM VPFVA  ED E+D
Sbjct: 243 GIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFVAAGEDHEQD 302

Query: 310 EDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
              V+AP+FV K P+QFKRQR IPIKAV
Sbjct: 303 STTVKAPTFVTKWPRQFKRQRTIPIKAV 330

BLAST of HG10023194 vs. NCBI nr
Match: KAA0058687.1 (mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa] >TYK10493.1 mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 522.7 bits (1345), Expect = 2.3e-144
Identity = 267/339 (78.76%), Postives = 296/339 (87.32%), Query Frame = 0

Query: 1   MKRNS-VEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNS +EE E E   +   SS FNNGIQW+RGRLIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVE---VEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVSETLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEMLT 240

Query: 241 GNLPWTASDKAGIVKQITED-YGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVP 300
           GN  W A+DK GIVK++TE+ +GMPKIP+GLS EA+ FLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + ++AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

BLAST of HG10023194 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.5e-50
Identity = 114/292 (39.04%), Postives = 172/292 (58.90%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W RG  IG G+F +V  A++  +      FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VR +T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRT----SKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    +        KIAD GLA R     +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S++D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFV 312
           +PKIPE LS E   FL  CFV++P  R+TAEML+   FV    + +  E+FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

BLAST of HG10023194 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.0e-38
Identity = 106/299 (35.45%), Postives = 159/299 (53.18%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W RGR++G+GS  +V+ A+   H S       I+AVKS+E+  SE LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAA--GHNS-----DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
               +I     E   + NG ++YNLL+E A  GTL       GG+ + E  V  YT+ I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRTSKTKPSYCLGGTL 206
           KGL +IH    VHCD+K +N+++    + KG    AKIAD G A+R      S  + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVI----SEKGE---AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 207 SYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDK----AGIVKQITEDYGMPK 266
           ++MAPE      Q   SDIWA+GC +++M+TG+ PWT +D       ++ ++      P+
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 267 IPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFVRAPSFVKKRP 322
           +P  L+ EA  FL+ C  R    R+TA  L+  PF+    D E     V  P  +   P
Sbjct: 241 LPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEP----VLVPGLISNSP 278

BLAST of HG10023194 vs. ExPASy Swiss-Prot
Match: Q9C5H5 (Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK5 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.3e-33
Identity = 98/302 (32.45%), Postives = 160/302 (52.98%), Query Frame = 0

Query: 4   NSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKP-----HISKYTIF 63
           +S     + + PL   S   N+  QW++G+LIG+G+FGSV++AS         + +  +F
Sbjct: 323 SSAASVPSPQAPLKLDSFPMNS--QWKKGKLIGRGTFGSVYVASNSETGALCAMKEVELF 382

Query: 64  PPIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 123
           P     KSAE    + L++E +   NL+  N ++Q F  E   D      + + LE    
Sbjct: 383 PD--DPKSAE--CIKQLEQEIKLLSNLQHPN-IVQYFGSETVEDR-----FFIYLEYVHP 442

Query: 124 GTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGR 183
           G++  +I +  G  + E+ VR++T+ I+ GL ++H  + VH D+K AN+L+         
Sbjct: 443 GSINKYIRDHCGT-MTESVVRNFTRHILSGLAYLHNKKTVHRDIKGANLLVDASG----- 502

Query: 184 RFVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSS-------DIWALGCL 243
             V K+AD G+A+  +  +    L G+  +MAPE +   +Q+ S+       DIW+LGC 
Sbjct: 503 --VVKLADFGMAKHLTGQRADLSLKGSPYWMAPELMQAVMQKDSNPDLAFAVDIWSLGCT 562

Query: 244 VLQMLTGNLPWTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEM 294
           +++M TG  PW+  + A  + ++  D   P IPE +S E   FL+ CF RNP  R TA M
Sbjct: 563 IIEMFTGKPPWSEFEGAAAMFKVMRD--SPPIPESMSPEGKDFLRLCFQRNPAERPTASM 602

BLAST of HG10023194 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 4.7e-31
Identity = 91/296 (30.74%), Postives = 151/296 (51.01%), Query Frame = 0

Query: 24  NNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYD 83
           N+ I+W++G+++G+G +GSV+L   K           + AVK  EI    +  K K    
Sbjct: 165 NHNIKWQKGQILGRGGYGSVYLGLNKD-------TGELFAVKQLEIVDINSDPKLK---- 224

Query: 84  NLKGCNSLIQCFAEEI----TTDHNGHMIY----------NLLLEVAAGGTLAHHINNTG 143
                 ++I  F++EI    +  H+  + Y          ++ LE   GG+++  +   G
Sbjct: 225 ------NMILSFSKEIEVMRSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFG 284

Query: 144 GKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGL 203
                EN ++ YTK I++GL  +H N  +H D+K ANIL+     TKG   + K++D G 
Sbjct: 285 --AFSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKG---IVKLSDFGC 344

Query: 204 ARRTSKTKPSY-CLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWT-ASDK 263
           ++  S     +  + GT  +MAPE +  +    SSDIW+LGC++++M T   PW+  ++ 
Sbjct: 345 SKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITEL 404

Query: 264 AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVED 304
           A ++  I     +P IP  +S EA  FL  CF R+P  R  A  L+  PF+  ++D
Sbjct: 405 AAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDD 434

BLAST of HG10023194 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-30
Identity = 89/305 (29.18%), Postives = 154/305 (50.49%), Query Frame = 0

Query: 13  ETPLITSSSSFNNGIQWERGRLIGKGSFGSVF----LASRKPHISKYTIFPPIMAVKSAE 72
           ++P   S+      I+W +G+LIG+G+FG+V+    L S +    K  +     A K   
Sbjct: 52  QSPPNNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKT 111

Query: 73  ISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNT 132
            +  + L++E +   NL   N  I  +   +  D       N+LLE   GG+++  +   
Sbjct: 112 QAHIQELEEEVKLLKNLSHPN--IVRYLGTVREDET----LNILLEFVPGGSISSLLEKF 171

Query: 133 GGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLG 192
           G    PE+ VR+YT  ++ GL ++H +  +H D+K ANIL+         +   K+AD G
Sbjct: 172 G--AFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILV-------DNQGCIKLADFG 231

Query: 193 LARRTSK---TKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTAS 252
            +++ ++      +  + GT  +MAPE ++ +    S+DIW++GC V++M+TG  PW+  
Sbjct: 232 ASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQ 291

Query: 253 DK-AGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQ 310
            K    +  I      P IP+ +S++A+ FL  C  + P  R TA  L+  PFV G + +
Sbjct: 292 YKEIAAIFHIGTTKSHPPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKE 341

BLAST of HG10023194 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.4e-147
Identity = 267/337 (79.23%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKR S+ E EA +      SS FNNGIQW++GRLIGKGSFGSVFLAS KPH +KY+IFPP
Sbjct: 1   MKRKSMLEKEAAQVE--DRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNGHMIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIVK++TE++ GMPKIPEGLS EA+ FLKNC VR P FRFTAEMLM VPFV
Sbjct: 241 RAWAATDKVGIVKEMTENFIGMPKIPEGLSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  ED E+D   V+AP+FV K P+QFKRQR IPIKAV
Sbjct: 301 AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

BLAST of HG10023194 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.1e-147
Identity = 265/337 (78.64%), Postives = 296/337 (87.83%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN + E EA +      S+ FNNGIQW+RGRLIGKGSFGSVFLA  KPH +KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQVE--EGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNG MIYNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GI++++TE++ GMPKIPEGLSAEA+ FLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGILQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V+AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

BLAST of HG10023194 vs. ExPASy TrEMBL
Match: A0A5D3CH22 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001440 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.6e-146
Identity = 266/337 (78.93%), Postives = 294/337 (87.24%), Query Frame = 0

Query: 1   MKRNSVEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHISKYTIFPP 60
           MKRN + E EA +      SS FNNGIQW+RGRLIGKGSFGSVFLA  KPH  KY+IFPP
Sbjct: 1   MKRNPMLEKEAAQVE--EGSSEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPP 60

Query: 61  IMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGT 120
           +MAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCF EEITTDHNG M YNLLLEVA GGT
Sbjct: 61  VMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGT 120

Query: 121 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKGRR 180
           LAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTTK R+
Sbjct: 121 LAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQ 180

Query: 181 FVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGN 240
           F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLTGN
Sbjct: 181 FIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGN 240

Query: 241 LPWTASDKAGIVKQITEDY-GMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFV 300
             W A+DK GIV+++TE++ GMPKIPEGLSAEA+ FLKNC VR P FRFTAEML+ VPFV
Sbjct: 241 RAWAATDKVGIVQEMTENFLGMPKIPEGLSAEATGFLKNCLVRKPEFRFTAEMLINVPFV 300

Query: 301 AGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           A  EDQE+D + V+AP+FV K P+QFKRQRIIPIKAV
Sbjct: 301 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

BLAST of HG10023194 vs. ExPASy TrEMBL
Match: A0A5D3CF40 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001430 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.1e-144
Identity = 267/339 (78.76%), Postives = 296/339 (87.32%), Query Frame = 0

Query: 1   MKRNS-VEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNS +EE E E   +   SS FNNGIQW+RGRLIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVE---VEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVSETLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCIVLEMLT 240

Query: 241 GNLPWTASDKAGIVKQITED-YGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVP 300
           GN  W A+DK GIVK++TE+ +GMPKIP+GLS EA+ FLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + ++AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

BLAST of HG10023194 vs. ExPASy TrEMBL
Match: A0A1S3CED5 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500026 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.5e-144
Identity = 267/339 (78.76%), Postives = 296/339 (87.32%), Query Frame = 0

Query: 1   MKRNS-VEEAEAEETPLITSSSSFNNGIQWERGRLIGKGSFGSVFLASRKPHIS-KYTIF 60
           MKRNS +EE E E   +   SS FNNGIQW+RGRLIGKGSFGSVFLAS KPHIS KY+IF
Sbjct: 1   MKRNSMLEELEVE---VEDGSSEFNNGIQWKRGRLIGKGSFGSVFLASLKPHISIKYSIF 60

Query: 61  PPIMAVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAG 120
           P +MAVKSAEISVSETLQKEKQNYDNLKGC+SLIQCF EEITTDHNGHMIYNLLLEVA G
Sbjct: 61  PSVMAVKSAEISVSETLQKEKQNYDNLKGCSSLIQCFGEEITTDHNGHMIYNLLLEVATG 120

Query: 121 GTLAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPR-NTTKG 180
           GTLAHHI NTGGKGL EN VR+YTKSIIKGLIHIH++QYVHCDLKPANILL+P+ NTT  
Sbjct: 121 GTLAHHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLIPKNNTTTN 180

Query: 181 RRFVAKIADLGLARRTSKTKPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLT 240
           R+F+AKIADLGLARRTSKTK SYCLGGT SYMAPETLID VQES+SDIWALGC+VL+MLT
Sbjct: 181 RQFIAKIADLGLARRTSKTKGSYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLT 240

Query: 241 GNLPWTASDKAGIVKQITED-YGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVP 300
           GN  W A+DK GIVK++TE+ +GMPKIP+GLS EA+ FLKNC VR P FRFTAEMLM VP
Sbjct: 241 GNRAWAATDKVGIVKEMTENLHGMPKIPDGLSVEATGFLKNCLVRKPEFRFTAEMLMNVP 300

Query: 301 FVAGVEDQEEDEDFVRAPSFVKKRPKQFKRQRIIPIKAV 336
           FVA   DQE+D + ++AP+ V K P+QFKRQRIIPIKAV
Sbjct: 301 FVAAAVDQEQDFNTMKAPTSVTKWPRQFKRQRIIPIKAV 336

BLAST of HG10023194 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-52
Identity = 122/295 (41.36%), Postives = 176/295 (59.66%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNL- 86
           ++W RG  IG G+F +V LA+R  + S    FPP+MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGE--FPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 87  KGCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSI 146
             CN +++CF E+ T + NG  ++NL LE A+ G+L  ++    G+G+PE+ VR +T S+
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 147 IKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRTSK-TKPSY--CL 206
           ++GL HIH N + HCDLK  NILL             KIAD GLA+R    T  +Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDG-------AVKIADFGLAKRIGDLTALNYGVQI 180

Query: 207 GGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWT---ASDKAGIVKQITEDYG 266
            GT  YMAPE++ D+   S  D+WALGC+V++M +G   W+    S+   ++ +I     
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDE 240

Query: 267 MPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQE---EDEDFV 312
           +P IPE LS +   FL  CFV++P  R+TAEML+  PFV    D +   ++EDFV
Sbjct: 241 VPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFV 285

BLAST of HG10023194 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 202.2 bits (513), Expect = 6.5e-52
Identity = 120/288 (41.67%), Postives = 170/288 (59.03%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W R   IG GSF +V LA+     SK   FP +MAVKS+ +  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
            C+ +++CF E  T + NG  IYNL LE A+GG+LA  I ++ G+ LPE EVR +T+SI+
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSS-GEALPEFEVRRFTRSIV 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRTSKTKPSYCLGGTL 206
           KGL HIH N + HCD+K  N+L+             KI+D GLA+R S  +    + GT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKRRS-GEVCVEIRGTP 180

Query: 207 SYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA-----GIVKQITEDYGMP 266
            YMAPE++     ES +DIWALGC V++M +G   W   D        ++ +I     +P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 267 KIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDED 310
           +IP  LS E   F+  CFV+N   R+TAEML+  PF+A V+D+  +ED
Sbjct: 241 RIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLA-VDDESGEED 275

BLAST of HG10023194 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 200.3 bits (508), Expect = 2.5e-51
Identity = 114/292 (39.04%), Postives = 172/292 (58.90%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLK 86
           ++W RG  IG G+F +V  A++  +      FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGD---FPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
            C  +I+C+ E+ T + NG  ++NLLLE A+ G+LA ++   GG+GLPE+ VR +T S++
Sbjct: 61  DCPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRT----SKTKPSYCL 206
           +GL HIH   + HCD+K ANILL    +        KIAD GLA R     +  + S  +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 207 GGTLSYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPWTASDKA---GIVKQITEDYG 266
            GT  YMAPE + D+   S++D+WALGC V++M +G   W+  + +    ++ +I     
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDE 240

Query: 267 MPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDEDFV 312
           +PKIPE LS E   FL  CFV++P  R+TAEML+   FV    + +  E+FV
Sbjct: 241 LPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFV 281

BLAST of HG10023194 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 172.6 bits (436), Expect = 5.5e-43
Identity = 104/283 (36.75%), Postives = 158/283 (55.83%), Query Frame = 0

Query: 27  IQWERGRLIGKGSFGSVFLASRKPHISKYTIFPPIMAVKSAEISVSETLQKEKQNYDNLK 86
           I W RG +IG+GS  +V +A         +    + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIA--------ISSSGELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 87  GCNSLIQCFAEEITTDHNGHMIYNLLLEVAAGGTLAHHINNTGGKGLPENEVRSYTKSII 146
             + +++     +T + NG ++YN+L+E  +GG L   I N+GGK LPE E+RSYT+ I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 147 KGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRFVAKIADLGLARRTSKTKPSYCLGGTL 206
            GL+++H+   VHCDLK  N+L+           V KIAD+G A+   K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLVEENG-------VLKIADMGCAKSVDKSE----FSGTP 182

Query: 207 SYMAPETLIDSVQESSSDIWALGCLVLQMLTGNLPW-TASDKAGIVKQITEDYGMPKIPE 266
           ++MAPE      Q   +D+WALGC +++M+TG+ PW   +D    + +I      P IP 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPA 242

Query: 267 GLSAEASRFLKNCFVRNPTFRFTAEMLMIVPFVAGVEDQEEDE 309
            +S +A  FLKNC   +   R+T E L+  PF   ++D EE +
Sbjct: 243 WISDKAKDFLKNCLKEDQKQRWTVEELLKHPF---LDDDEESQ 260

BLAST of HG10023194 vs. TAIR 10
Match: AT5G27510.1 (Protein kinase superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 5.2e-41
Identity = 94/237 (39.66%), Postives = 134/237 (56.54%), Query Frame = 0

Query: 63  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFAEEITT--DHNGHMIYNLLLEVAAGGT 122
           AVKS++     +L KE      LKGC  +IQCF  ++    D  G+ +Y LLLE A+ G+
Sbjct: 35  AVKSSD--DENSLLKEFHILSELKGCPRIIQCFGNDLEEGFDDKGNRVYKLLLEYASEGS 94

Query: 123 LAHHINNTGGKGLPENEVRSYTKSIIKGLIHIHKNQYVHCDLKPANILLVPRNTTKGRRF 182
           L+  +NN   + LP+  +R +T+ I++GL+ IH + YVHCDLKP N+L+ P     G  +
Sbjct: 95  LSDFMNNCVDRKLPDLMIRDFTRMILQGLVSIHSHGYVHCDLKPENVLVFP----CGDSY 154

Query: 183 VAKIADLGLARRTSKT----KPSYCLGGTLSYMAPETLIDSVQESSSDIWALGCLVLQML 242
             KI+D GL+ +  +     K  Y   GTL+YM PE+L D V   + D+W+LGCLVL+M 
Sbjct: 155 EVKISDFGLSLQVGEVPDHWKIEYPFVGTLNYMPPESLHDGVANKTLDLWSLGCLVLEMY 214

Query: 243 TGNLPWTASDKAGIVKQITEDYGMPKIPEGLSAEASRFLKNCFVRNPTFRFTAEMLM 294
               PW        V  I  +   P+IPE L  +A  F++ CF RNP  R TA  L+
Sbjct: 215 VCKKPWIGFIPEDFV-YILSNGNPPEIPESLPCDARAFIQKCFSRNPKERGTASELL 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004136102.21.1e-14678.93mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus][more]
XP_008461430.11.5e-14678.64PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo][more]
KAA0058688.13.3e-14678.93mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]... [more]
KAE8646420.11.6e-14579.57hypothetical protein Csa_015819 [Cucumis sativus][more]
KAA0058687.12.3e-14478.76mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo var. makuwa]... [more]
Match NameE-valueIdentityDescription
Q9SND63.5e-5039.04Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808884.0e-3835.45Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9C5H52.3e-3332.45Mitogen-activated protein kinase kinase kinase 5 OS=Arabidopsis thaliana OX=3702... [more]
Q54R824.7e-3130.74Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q9FZ361.8e-3029.18Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0KBM32.4e-14779.23Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430... [more]
A0A1S3CFZ67.1e-14778.64mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
A0A5D3CH221.6e-14678.93Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A5D3CF401.1e-14478.76Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw... [more]
A0A1S3CED51.5e-14478.76mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G67080.11.0e-5241.36mitogen-activated protein kinase kinase kinase 19 [more]
AT4G36950.16.5e-5241.67mitogen-activated protein kinase kinase kinase 21 [more]
AT3G50310.12.5e-5139.04mitogen-activated protein kinase kinase kinase 20 [more]
AT4G26890.15.5e-4336.75mitogen-activated protein kinase kinase kinase 16 [more]
AT5G27510.15.2e-4139.66Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 29..298
e-value: 1.8E-53
score: 193.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 30..297
e-value: 2.9E-45
score: 154.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 29..298
score: 36.72509
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 25..330
e-value: 9.0E-55
score: 188.1
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 106..288
e-value: 5.5E-9
score: 32.7
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 106..288
e-value: 5.5E-9
score: 32.7
NoneNo IPR availablePIRSRPIRSR037568-1PIRSR037568-1coord: 31..259
e-value: 7.0E-11
score: 38.1
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 26..313
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 26..313
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 35..65
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 157..169
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 28..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023194.1HG10023194.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity