HG10023186 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023186 vs. NCBI nr
Match: XP_038897161.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.6e-291
Identity = 511/542 (94.28%), Postives = 524/542 (96.68%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA+ESNQTNEFIFRSKLPDIHIPNHLPLHDY+FQNL KFASRPCLINGATGDVYS+HDVQ
Sbjct: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGC HFSVLSGADE+HAP+V+FS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCG+RAG AIMIMQKFDIVSL QLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS
Sbjct: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVRAKFP AIL QGYGMTEAGPVLTMSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG IDD DELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM D EAGEVPV
Sbjct: 421 IGFIDD-DELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+KE+D  ITEE+VK FITKQVVFYKRLK V+FVN IPKAPSGKILRKELRTKLAS A
Sbjct: 481 AFVVKESDGTITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 541

BLAST of HG10023186 vs. NCBI nr
Match: XP_038897021.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 1008.1 bits (2605), Expect = 3.0e-290
Identity = 508/542 (93.73%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNL KFASRPCLINGATGDVYSYHDV 
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLPKFASRPCLINGATGDVYSYHDVH 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIV VDFAVEGC HFSVLSGADES+AP V+FS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVSVDFAVEGCLHFSVLSGADESNAPPVDFSP 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY GDDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHGDDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G +IMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGASIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+LGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD+DELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEE+VKQFI+KQVVFYKRLKRVFFVNTIPKAPSGKILRKELR +LAS A
Sbjct: 481 AFVVKTNGGAITEEEVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRARLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. NCBI nr
Match: XP_004135893.3 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KAE8646417.1 hypothetical protein Csa_015838 [Cucumis sativus])

HSP 1 Score: 995.0 bits (2571), Expect = 2.6e-286
Identity = 500/542 (92.25%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVDFAVEGC HFSVLSGADES AP V+FS+
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G AI+IMQKFDIVSLLQLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+LGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD+DELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEE+VKQFITKQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. NCBI nr
Match: XP_004135894.2 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KGN45189.1 hypothetical protein Csa_016278 [Cucumis sativus])

HSP 1 Score: 994.6 bits (2570), Expect = 3.4e-286
Identity = 502/542 (92.62%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGV+IVCVDFAVEGC HFSVLSGADES AP V+FS+
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G AIMIMQKFDIV+LLQLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTAILGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD++ELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AI+EE+VKQFI KQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 994.6 bits (2570), Expect = 3.4e-286
Identity = 496/542 (91.51%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MAVESNQT+EFIFRSKLPDIHIPNHLPLH+YVFQN+SKF SRPCLINGATGD+YSYHDVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           L +RRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGC H+SVLSGADESH P VEF+A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY G DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLRAG AI+IMQKF+IVSLL+LIEK+++SIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTA+LGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG IDD+DELFI+DRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPD +AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEEDVKQFI+KQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of HG10023186 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 6.3e-227
Identity = 385/534 (72.10%), Postives = 450/534 (84.27%), Query Frame = 0

Query: 10  EFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTARRVAAG 69
           + IFRSKLPDI+IPNHLPLH Y F+N+S+F+SRPCLINGA   +Y+Y DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP  T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSADDVVALPY 189
           VT AC  ++VKD A EN VKI+C+D A EGC HFSVL+ A+E   P VE   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   +DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSVRVLKSG 309
           LR G AI+IMQKFDIVS L+LI++Y+++I P VPPI LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGTVVRNAE 369
            APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEPF++K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGLIDDND 429
           MKIVDP+TG SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG IDD+D
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVIKEND 489
           ELFI+DRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVPVAFV++ N 
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 DAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSAYN 543
             ITE++VK FI+KQV+FYKR+KRVFFV+ IPK+PSGKILRK+LR KLA+   N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of HG10023186 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 1.4e-226
Identity = 390/546 (71.43%), Postives = 457/546 (83.70%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           +A+E  Q  + IFRSKLPDI+IP +LPLH Y F+N+SKF+SRPCLINGAT ++++Y DV+
Sbjct: 5   VAIE-EQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           L +RRV +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF T+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFA-----VEGCFHFSVLSGADESHAP 180
           AKA+NAKLI+T  C+ D+VKD A ENGVKI+ +D            HFS L+GADE+  P
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMP 184

Query: 181 SVEFSADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLP 240
            VE S D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   DDV+LCVLP
Sbjct: 185 KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 244

Query: 241 FFHIYSLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEF 300
            FHIYSLNS+LLCGLRAG+ I+IMQKF+IV  L+LI+KY+++I P VPPI LAIAKS+  
Sbjct: 245 LFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVV 304

Query: 301 EKYDVSSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQ 360
           + YD+SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF 
Sbjct: 305 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 364

Query: 361 VKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEG 420
           +K+GACGTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGYLND E+T RTIDKEG
Sbjct: 365 IKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEG 424

Query: 421 WLHTGDIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVE 480
           WLHTGDIG IDD+DELFI+DRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D  
Sbjct: 425 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEA 484

Query: 481 AGEVPVAFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRT 540
           AGEVPVAFV+K N   ITE+++KQFI+KQV+FYKR+ RVFFV  IPKAPSGKILRK+LR 
Sbjct: 485 AGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRA 544

BLAST of HG10023186 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 9.1e-226
Identity = 381/543 (70.17%), Postives = 451/543 (83.06%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           M  E+NQ  + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LT+R+VAAGL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP  T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFS 180
           AKA+NAKLI+T ACF ++VKD A +N + ++C+D A EGC HFS L+ ADE   P V+  
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIY 240
           +DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDV 300
           SLNS+LLCGLR G AI+IMQKFDIV   +LIEKY+++I P VPPI LAIAKS   + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG ID++DELFI+DRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASS 540
           VAFV++ N   ITE++VK F++KQV+FYKR+KRVFFV T+PK+PSGKILRK+LR +LA+ 
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 VPN 544

BLAST of HG10023186 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 9.1e-226
Identity = 379/540 (70.19%), Postives = 449/540 (83.15%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTA 63
           E+ Q+ + IFRSKLPDI+IP HLPLH Y F+N+S+F+SRPCLINGA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP  T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSADD 183
           ++AK+I+T +CF  +VKD A EN VK++C+D A EGC HFS L+ +DE   P V+   DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSV 303
           SILLCGLR G AI+IMQKFDI   L+LI+KY++SI P VPPI LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 LIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAF 483
            ID++DELFI+DRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSAYN 543
           V++ N  AITE++VK FI+KQV+FYKR+KRVFFV T+PK+PSGKILRK+LR +LA+   N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of HG10023186 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 4.2e-223
Identity = 378/543 (69.61%), Postives = 446/543 (82.14%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           M +E+ Q+ + IFRSKLPDI+IP HLPLH Y F+NLS+F SRPCLI+GA   +Y+Y +V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LT+R+VA GL+ LGI++ D +M LLPN PEFVF F+GASY GAI T ANP  T  E+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLA-ENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFS 180
           AKA++AK+++T ACF  +VKD A EN +K++CVD A EGC HFS L  +DE   P V+  
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 ADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   DDV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDV 300
           SLNS+LLC LR G AI+IMQKFDI   L+LI K++++I P VPPI LAIAKS     YD+
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGA 360
           SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVP 480
           DIG IDD+DELFI+DRLKELIK+K FQVAPAELEALLI HP +SDAAV+ M D +AGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASS 540
           VAFV++ N   ITE++VK FI+KQV+FYKR+KRVFFV T+PK+PSGKILRK+LR +LA+ 
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

Query: 541 AYN 543
             N
Sbjct: 543 ISN 545

BLAST of HG10023186 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 496/542 (91.51%), Postives = 521/542 (96.13%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MAVESNQT+EFIFRSKLPDIHIPNHLPLH+YVFQN+SKF SRPCLINGATGD+YSYHDVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           L +RRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGC H+SVLSGADESH P VEF+A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY G DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLRAG AI+IMQKF+IVSLL+LIEK+++SIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTA+LGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG IDD+DELFI+DRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPD +AGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEEDVKQFI+KQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of HG10023186 vs. ExPASy TrEMBL
Match: A0A0A0K8H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 502/542 (92.62%), Postives = 522/542 (96.31%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGV+IVCVDFAVEGC HFSVLSGADES AP V+FS+
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G AIMIMQKFDIV+LLQLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTAILGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD++ELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AI+EE+VKQFI KQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. ExPASy TrEMBL
Match: A0A0A0K658 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 4.8e-286
Identity = 499/542 (92.07%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESN+TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGD Y+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGV++VCVDFAVEGC HFSVLSGADES AP V+FS+
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G AI+IMQKFDIVSLLQLIEK+RISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP A+LGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD+DELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEE+VKQFITKQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. ExPASy TrEMBL
Match: A0A5A7US61 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001380 PE=4 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.0e-285
Identity = 498/542 (91.88%), Postives = 517/542 (95.39%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MA ESNQT EFIFRSKLPDIHIPNHLPLH YVFQN+ KFASRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGC HFSVLSGADES AP V+FS+
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYY  DDVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLR G  I+IMQKFDIVSLLQLIEK++ISIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTA+LGQGYGMTEAGPVL+MSLAFAKEPFQVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD+DELFI+DRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEE+VKQFITKQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLA  A
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of HG10023186 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 989.6 bits (2557), Expect = 5.3e-285
Identity = 492/542 (90.77%), Postives = 520/542 (95.94%), Query Frame = 0

Query: 1   MAVESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQ 60
           MAVESNQT+EFIFRSKLPDIHIPNHLPLH+YVFQN+SKF SRPCLINGATGDVYSYHDVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQ 120
           L +RRVAAGLH+LGIKK DVVMNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCFHFSVLSGADESHAPSVEFSA 180
           AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGC H+SVLSGADESH P+VEF+A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY G DVILCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300
           LNSILLCGLRAG AI+IMQKF+I+SLL+LIEK+++SIMPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPTA+LGQGYGMTEAGPVLTMSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480
           IG +DD+DELFI+DRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIGMPD +AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSA 540
           AFV+K N  AITEEDVKQFI+KQVVFYKRLKRVFFVN IPKAPSGKILRKELR KLAS A
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           +N
Sbjct: 541 FN 542

BLAST of HG10023186 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 749.2 bits (1933), Expect = 2.3e-216
Identity = 373/543 (68.69%), Postives = 445/543 (81.95%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG VY+Y DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCFHFSVLSGADESHA---PS 184
           N KLI+T A + D++K L  ++GV IVC+D        EGC  F+ L+ +    +    S
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VEFSADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPF 244
           VE S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFE 304
           FHIY+LNSI+LCGLR G AI+IM KF+I  LL+LI++ ++++ P+VPPI LAIAKSSE E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIGLIDD+DELFI+DRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTK 539
           GEVPVAFV+K  D  ++E+DVKQF++KQVVFYKR+ +VFF  +IPKAPSGKILRK+LR K
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of HG10023186 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 741.1 bits (1912), Expect = 6.3e-214
Identity = 361/539 (66.98%), Postives = 449/539 (83.30%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLHDY+F+N+S+FA++PCLING TG+VY+Y DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKAANAK 127
           AGLH+LG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDFAV--EGCFHFSVLSGADESHAPSV--EFSADDV 187
           LIVT + + D++K+L  +GV IV  D     E C  FS L+ ++E    S+  + S +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVSSVR 307
           I+LC LR G  I+IM KF+I  LL+ I++ ++++  +VPPI LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQVKAGACGTV 367
           ++KSG APLGKELEDA+ AKFP A LGQGYGMTEAGPVL MSL FAKEPF VK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGL 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+G 
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFV 487
           IDD+DELFI+DRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M + +AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 IKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRTKLASSAYN 543
           ++  D  I+E+++KQF++KQVVFYKR+ +VFF ++IPKAPSGKILRK+LR +LA+   N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of HG10023186 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 698.7 bits (1802), Expect = 3.6e-201
Identity = 348/509 (68.37%), Postives = 415/509 (81.53%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG VY+Y DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCFHFSVLSGADESHA---PS 184
           N KLI+T A + D++K L  ++GV IVC+D        EGC  F+ L+ +    +    S
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VEFSADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPF 244
           VE S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFE 304
           FHIY+LNSI+LCGLR G AI+IM KF+I  LL+LI++ ++++ P+VPPI LAIAKSSE E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEA 484
           LHTGDIGLIDD+DELFI+DRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVIKENDDAITEEDVKQFITKQV 505
           GEVPVAFV+K  D  ++E+DVKQF++KQV
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of HG10023186 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 669.1 bits (1725), Expect = 3.0e-192
Identity = 327/546 (59.89%), Postives = 419/546 (76.74%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLS----KFASRPCLINGATGDVYSYHDV 63
           E   +++FIFRSKLPDI IPNHLPL DYVFQ  S      +S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPF T  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLAENGVKIVCVD---------FAVEGCFHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          + +GC  F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHAPSVEFSADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVIL 243
           +     + S +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  +DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R G A++I+ +F++  +++LI++Y+++++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SSEFEKYDVSSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L KELEDAVR KFP AI GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF+ K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIG +DD+DE+FI+DRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVIKENDDAITEEDVKQFITKQVVFYKRLKRVFFVNTIPKAPSGKILRK 537
            D  A EVPVAFV +     +TE+DVK ++ KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of HG10023186 vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 656.0 bits (1691), Expect = 2.7e-188
Identity = 327/473 (69.13%), Postives = 387/473 (81.82%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYHDVQLTAR 64
           +N  ++ IFRSKLPDI+IPNHL LHDY+FQN+S+FA++PCLING TG VY+Y DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFCTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDLA-ENGVKIVCVDF-----AVEGCFHFSVLSGADESHA---PS 184
           N KLI+T A + D++K L  ++GV IVC+D        EGC  F+ L+ +    +    S
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VEFSADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPF 244
           VE S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGTAIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSSEFE 304
           FHIY+LNSI+LCGLR G AI+IM KF+I  LL+LI++ ++++ P+VPPI LAIAKSSE E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELEDAVRAKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGLIDDNDELFIIDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVI 469
           LHTGDIGLIDD+DELFI+DRLKELIK+K FQVAPAELEALLI HP ++D AV+
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVV 489