HG10023087 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023087
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCytochrome P450, putative
LocationChr05: 31089468 .. 31090007 (-)
RNA-Seq ExpressionHG10023087
SyntenyHG10023087
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGGCAACCTTTCCTCCTCCTCCTCCTTGCCCCCTCCAATTTCCGTCGGAAACTTCGGGCTCTGGCCACTCCTCGATGTCCAAATCTTGGAGATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCGTTAGACAGAAGAGACATTATGACTTGCCTGTGTGGCCAATCGTTGGTATGTTACCGGCTTTGATTACAGCAACACGTGAAAATATCTACGAGTGGATTACTGACCTCTTATGTCGTCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGTTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCTTCCTACCCAAAAGGGTCATTCTTCCGCGACACAGTGAGAGACCTACTCGGTGACGGAATATTTGGGGCAGACGACGAGACGTGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGATTTCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTGCACTCAAGTTGA

mRNA sequence

ATGGATTCCGGCAACCTTTCCTCCTCCTCCTCCTTGCCCCCTCCAATTTCCGTCGGAAACTTCGGGCTCTGGCCACTCCTCGATGTCCAAATCTTGGAGATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCGTTAGACAGAAGAGACATTATGACTTGCCTGTGTGGCCAATCGTTGGTATGTTACCGGCTTTGATTACAGCAACACGTGAAAATATCTACGAGTGGATTACTGACCTCTTATGTCGTCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGTTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCTTCCTACCCAAAAGGGTCATTCTTCCGCGACACAGTGAGAGACCTACTCGGTGACGGAATATTTGGGGCAGACGACGAGACGTGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGATTTCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTGCACTCAAGTTGA

Coding sequence (CDS)

ATGGATTCCGGCAACCTTTCCTCCTCCTCCTCCTTGCCCCCTCCAATTTCCGTCGGAAACTTCGGGCTCTGGCCACTCCTCGATGTCCAAATCTTGGAGATCTCTTTCGCTCTTTTTGTGTTCTTAGTCATCCATGTCGTTAGACAGAAGAGACATTATGACTTGCCTGTGTGGCCAATCGTTGGTATGTTACCGGCTTTGATTACAGCAACACGTGAAAATATCTACGAGTGGATTACTGACCTCTTATGTCGTCACAAGGGCACGTTTCAATTTCGAGGCCCCTGGTTTACTAGTCTCAATTGTGTCGTCACTGCTGATCCTCGAAACTTGGAGCACCTTCTCAAGACCAAATTTTCTTCCTACCCAAAAGGGTCATTCTTCCGCGACACAGTGAGAGACCTACTCGGTGACGGAATATTTGGGGCAGACGACGAGACGTGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCGAGATTTCGACAAATGACGGTGGAGTCGTTGGTGGAGCTCGTGCACTCAAGTTGA

Protein sequence

MDSGNLSSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSS
Homology
BLAST of HG10023087 vs. NCBI nr
Match: KAA0058578.1 (cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450 86B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 332.4 bits (851), Expect = 2.4e-87
Identity = 166/180 (92.22%), Postives = 170/180 (94.44%), Query Frame = 0

Query: 1   MDSGNL--SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVW 60
           MDSGNL  SSSSSLPP ISV +FGL  LLDVQILEISFALFVFLVIHVVRQKR + LP+W
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 61  PIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

BLAST of HG10023087 vs. NCBI nr
Match: XP_004135954.3 (cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_015511 [Cucumis sativus])

HSP 1 Score: 331.6 bits (849), Expect = 4.1e-87
Identity = 164/181 (90.61%), Postives = 170/181 (93.92%), Query Frame = 0

Query: 1   MDSGNL---SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPV 60
           MDSGNL   SSSSS  PPIS+G+FGL  LLDVQILEI+FALFVFLVIHVVRQKR + LP+
Sbjct: 39  MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 98

Query: 61  WPIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKT 120
           WPIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKT
Sbjct: 99  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158

Query: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 179
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 159 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218

BLAST of HG10023087 vs. NCBI nr
Match: XP_008461361.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 330.9 bits (847), Expect = 7.0e-87
Identity = 165/180 (91.67%), Postives = 169/180 (93.89%), Query Frame = 0

Query: 1   MDSGNL--SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVW 60
           MDSGNL  SSSSSLPP ISV +FGL  LLDVQILEISFALFVFLVIHVVRQKR + LP+W
Sbjct: 39  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 98

Query: 61  PIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKTK
Sbjct: 99  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 158

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HS
Sbjct: 159 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHS 218

BLAST of HG10023087 vs. NCBI nr
Match: XP_038899430.1 (cytochrome P450 86B1-like [Benincasa hispida])

HSP 1 Score: 330.5 bits (846), Expect = 9.1e-87
Identity = 165/179 (92.18%), Postives = 170/179 (94.97%), Query Frame = 0

Query: 1   MDSGNLSSSSS-LPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWP 60
           MDSGNLSSSSS LPPPISV NF L PLLDVQILEISFALFVFLVIHVVRQKR YDLPVWP
Sbjct: 15  MDSGNLSSSSSPLPPPISVANFRLRPLLDVQILEISFALFVFLVIHVVRQKRRYDLPVWP 74

Query: 61  IVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKF 120
           +VGMLPALITA R NIYEWIT+LL RHKGTFQFRGPW +SL+CVVTADPRNLEHLLKTKF
Sbjct: 75  LVGMLPALITAARGNIYEWITELLHRHKGTFQFRGPWLSSLHCVVTADPRNLEHLLKTKF 134

Query: 121 SSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           SSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTV+SLVELVHS
Sbjct: 135 SSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVDSLVELVHS 193

BLAST of HG10023087 vs. NCBI nr
Match: XP_022954102.1 (cytochrome P450 86B1-like [Cucurbita moschata])

HSP 1 Score: 328.2 bits (840), Expect = 4.5e-86
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 0

Query: 1   MDSGNLSSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPI 60
           MDSGNLSSSS+LP  +S GNFGLWPLLD QILEISFALFVFL IHVVRQ+R Y LPVWPI
Sbjct: 15  MDSGNLSSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIHVVRQQRRYGLPVWPI 74

Query: 61  VGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA R N+YEWITDLL  HKGTFQFRGPWF+SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 135 SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 192

BLAST of HG10023087 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.1e-61
Identity = 113/171 (66.08%), Postives = 136/171 (79.53%), Query Frame = 0

Query: 7   SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPIVGMLPA 66
           SSSSS  P +S     L+ L DVQILE+  A+FVF+ IH +RQK++  LPVWP +GMLP+
Sbjct: 17  SSSSSSDPLVS---RRLFLLRDVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 67  LITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGS 126
           L    R NIYEW++D+LC   GTFQFRGPWF+SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 127 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 178
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVH 184

BLAST of HG10023087 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.5e-23
Identity = 63/146 (43.15%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 27  LDVQILEISFALFVFLVIHVVRQKRHYDLP-VWPIVGMLPALITATRENIYEWITDLLCR 86
           +D     I   L V  +I  V  +R Y  P VWP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALIT-NAHRMHDFIADNLRM 60

Query: 87  HKGTFQ---FRGPWFTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 146
             GT+Q   F  P+      +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 147 FGADDETWQRQRKVASIEFHSARFRQ 167
           F +D +TW+ QRK A++EF +   RQ
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQ 145

BLAST of HG10023087 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.8e-22
Identity = 53/115 (46.09%), Postives = 72/115 (62.61%), Query Frame = 0

Query: 57  VWPIVGMLPALITATRENIYEWITDLLCRHKGTFQ---FRGPWFTSLN--CVVTADPRNL 116
           VWP++G LP LI   R+ ++EWIT+ L    GT+Q   F  P+         VT DP+NL
Sbjct: 32  VWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDPKNL 91

Query: 117 EHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQ 167
           EH+LKT+F +YPKG  ++    DLLG GIF +D +TW  QRK A++EF +   RQ
Sbjct: 92  EHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQ 145

BLAST of HG10023087 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.4e-20
Identity = 51/114 (44.74%), Postives = 69/114 (60.53%), Query Frame = 0

Query: 57  VWPIVGMLPALITATRENIYEWITDLLCRHKGTFQ---FRGPWFTSLNCV-VTADPRNLE 116
           +WP+VG LP LI      ++EWI D L    GT+Q      P+      V VT DP+NLE
Sbjct: 35  MWPVVGSLPGLI-ENGNRMHEWIADNLRACSGTYQTCICAIPFLARKQLVTVTCDPKNLE 94

Query: 117 HLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQ 167
           H+LK +F +YPKG  ++    DLLG+GIF +D +TW  QRK A++EF +   RQ
Sbjct: 95  HILKVRFDNYPKGPTWQAVFHDLLGEGIFNSDGDTWLFQRKTAALEFTTRTLRQ 147

BLAST of HG10023087 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.4e-20
Identity = 52/115 (45.22%), Postives = 69/115 (60.00%), Query Frame = 0

Query: 57  VWPIVGMLPALITATRENIYEWITDLLCRHKGTFQ---FRGPWFTSLN--CVVTADPRNL 116
           VWPI+G LP LI    E +++WI+D L    GT+Q      P+         VT DPRNL
Sbjct: 32  VWPILGSLPGLI-ENCERMHDWISDNLRACSGTYQTCICAIPFLAKKQGLVTVTCDPRNL 91

Query: 117 EHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQ 167
           EH+LK +F +YPKG  ++    DLLG GIF +D +TW  QRK A++EF +   RQ
Sbjct: 92  EHILKNRFDNYPKGPTWQAVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQ 145

BLAST of HG10023087 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.2e-87
Identity = 166/180 (92.22%), Postives = 170/180 (94.44%), Query Frame = 0

Query: 1   MDSGNL--SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVW 60
           MDSGNL  SSSSSLPP ISV +FGL  LLDVQILEISFALFVFLVIHVVRQKR + LP+W
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 61  PIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

BLAST of HG10023087 vs. ExPASy TrEMBL
Match: A0A0A0K879 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.0e-87
Identity = 164/181 (90.61%), Postives = 170/181 (93.92%), Query Frame = 0

Query: 1   MDSGNL---SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPV 60
           MDSGNL   SSSSS  PPIS+G+FGL  LLDVQILEI+FALFVFLVIHVVRQKR + LP+
Sbjct: 1   MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60

Query: 61  WPIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKT 120
           WPIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 179
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

BLAST of HG10023087 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.4e-87
Identity = 165/180 (91.67%), Postives = 169/180 (93.89%), Query Frame = 0

Query: 1   MDSGNL--SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVW 60
           MDSGNL  SSSSSLPP ISV +FGL  LLDVQILEISFALFVFLVIHVVRQKR + LP+W
Sbjct: 39  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 98

Query: 61  PIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATR N+YEWITDLLCRHKGTFQFRGPW TSLNCVVTADPRNLEHLLKTK
Sbjct: 99  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 158

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HS
Sbjct: 159 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHS 218

BLAST of HG10023087 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.2e-86
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 0

Query: 1   MDSGNLSSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPI 60
           MDSGNLSSSS+LP  +S GNFGLWPLLD QILEISFALFVFL IHVVRQ+R Y LPVWPI
Sbjct: 15  MDSGNLSSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIHVVRQQRRYGLPVWPI 74

Query: 61  VGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA R N+YEWITDLL  HKGTFQFRGPWF+SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
           SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 135 SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 192

BLAST of HG10023087 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.0e-84
Identity = 156/178 (87.64%), Postives = 164/178 (92.13%), Query Frame = 0

Query: 1   MDSGNLSSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPI 60
           MDSGNLSSSS+LP  +S  NF LWPLLDVQILE+SFALFVFL IHV+RQ+R Y L VWPI
Sbjct: 15  MDSGNLSSSSALPAVVSAENFRLWPLLDVQILELSFALFVFLAIHVIRQQRRYGLSVWPI 74

Query: 61  VGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA R N+YEWITDLL RHKGTFQFRGPWF+SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 179
            YPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 135 YYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 192

BLAST of HG10023087 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 237.7 bits (605), Expect = 7.5e-63
Identity = 113/171 (66.08%), Postives = 136/171 (79.53%), Query Frame = 0

Query: 7   SSSSSLPPPISVGNFGLWPLLDVQILEISFALFVFLVIHVVRQKRHYDLPVWPIVGMLPA 66
           SSSSS  P +S     L+ L DVQILE+  A+FVF+ IH +RQK++  LPVWP +GMLP+
Sbjct: 17  SSSSSSDPLVS---RRLFLLRDVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 67  LITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGS 126
           L    R NIYEW++D+LC   GTFQFRGPWF+SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 127 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 178
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVH 184

BLAST of HG10023087 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-53
Identity = 89/124 (71.77%), Postives = 110/124 (88.71%), Query Frame = 0

Query: 55  LPVWPIVGMLPALITATRENIYEWITDLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHL 114
           +PVWP+VGMLP+LI+A R NIYEW++D+L    GTF+FRGPWF++LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 115 LKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVE 174
           LKT+FS YPKGS+FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSA+FRQ+T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 175 LVHS 179
           LVH+
Sbjct: 121 LVHN 124

BLAST of HG10023087 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-31
Identity = 60/156 (38.46%), Postives = 100/156 (64.10%), Query Frame = 0

Query: 23  LWPLLDVQILEISFALFVFLVIHVVRQK--RHYDLPVWPIVGMLPALITATRENIYEWIT 82
           +W    +++ +++ AL    ++  +R+K        +WP++G++P ++   + +++ W T
Sbjct: 9   VWVYTHLRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCT 68

Query: 83  DLLCRHKGTFQFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 142
             + R  GTF +RG WF     ++TADP N+EH+LKT F +YPKG+F+R+  RDLL DGI
Sbjct: 69  RCVVRSGGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGI 128

Query: 143 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELV 177
           F ADDE W+ +R+VA  E HS+RF + T  ++ +LV
Sbjct: 129 FNADDELWKEERRVAKTEMHSSRFLEHTFTTMRDLV 163

BLAST of HG10023087 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 112.5 bits (280), Expect = 3.6e-25
Identity = 55/146 (37.67%), Postives = 84/146 (57.53%), Query Frame = 0

Query: 33  EISFALFVFLVIHVVRQK--RHYDLPVWPIVGMLPALITATRENIYEWITDLLCRHKGTF 92
           ++S AL    V   VR+K  +     +WP+ G+ P      R ++Y W T  L + +GTF
Sbjct: 22  DVSLALLGLFVFCCVREKVTKKLGPTIWPVFGITPEFF-FHRNDVYGWATRCLKKCRGTF 81

Query: 93  QFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQR 152
            + G W       VT  P N+E++LKT F ++PKG+FF++   DLL DGIF AD E+W+ 
Sbjct: 82  LYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKE 141

Query: 153 QRKVASIEFHSARFRQMTVESLVELV 177
           QR++   E HS RF + + ++  +LV
Sbjct: 142 QRRIIITEMHSTRFVEHSFQTTQDLV 166

BLAST of HG10023087 vs. TAIR 10
Match: AT1G13140.2 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 112.5 bits (280), Expect = 3.6e-25
Identity = 55/146 (37.67%), Postives = 84/146 (57.53%), Query Frame = 0

Query: 33  EISFALFVFLVIHVVRQK--RHYDLPVWPIVGMLPALITATRENIYEWITDLLCRHKGTF 92
           ++S AL    V   VR+K  +     +WP+ G+ P      R ++Y W T  L + +GTF
Sbjct: 22  DVSLALLGLFVFCCVREKVTKKLGPTIWPVFGITPEFF-FHRNDVYGWATRCLKKCRGTF 81

Query: 93  QFRGPWFTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQR 152
            + G W       VT  P N+E++LKT F ++PKG+FF++   DLL DGIF AD E+W+ 
Sbjct: 82  LYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKE 141

Query: 153 QRKVASIEFHSARFRQMTVESLVELV 177
           QR++   E HS RF + + ++  +LV
Sbjct: 142 QRRIIITEMHSTRFVEHSFQTTQDLV 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0058578.12.4e-8792.22cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450... [more]
XP_004135954.34.1e-8790.61cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_01... [more]
XP_008461361.17.0e-8791.67PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo][more]
XP_038899430.19.1e-8792.18cytochrome P450 86B1-like [Benincasa hispida][more]
XP_022954102.14.5e-8689.33cytochrome P450 86B1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FMY11.1e-6166.08Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD61.5e-2343.15Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Q9LMM14.8e-2246.09Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
B3RFJ63.4e-2044.74Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
O808233.4e-2045.22Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UTT41.2e-8792.22Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0K8792.0e-8790.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1[more]
A0A1S3CEY83.4e-8791.67LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1GQ362.2e-8689.33cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A6J1JMG12.0e-8487.64cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23190.17.5e-6366.08cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.11.4e-5371.77Cytochrome P450 superfamily protein [more]
AT1G24540.11.2e-3138.46cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.13.6e-2537.67cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT1G13140.23.6e-2537.67cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PFAMPF00067p450coord: 56..174
e-value: 7.4E-7
score: 28.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 49..178
e-value: 2.7E-17
score: 64.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 56..168
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 18..178
NoneNo IPR availablePANTHERPTHR24296:SF217BNAC02G41740D PROTEINcoord: 18..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023087.1HG10023087.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0070330 aromatase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen