HG10023050 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023050
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr05: 30789022 .. 30790796 (-)
RNA-Seq ExpressionHG10023050
SyntenyHG10023050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAGTTTCGTGTTGTCATTTGCAATTTGGTTATCTTTTATTTCTTGTCTTCTTTCTTCTTCTTCTCAAGCTAGATTGCATCATCACACCAAACAAAAACATTCTCACCACCATAAACATAGCTACATTTCGCACCCAAATTCTCCTTCTCCTTCTCCTTCTAATTCACCCGACGACGGAGATTACGTTAACGAGACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGTGTTGGAGATGACACGGGGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAAAGTGAAGACTCTGCCATCATGCTAGTTCCCTATGGCTTCTCATTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCACGGTAATCTCATGTTACGAGTCGACGGCACGTTGATGCCACCGGATGGCCCCGATGCATGGCCGAAGAATGCTAGTCGCCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGAAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAAGTATTCAATCTTCTTCAATGTTTATATAAATGCCTCCAATTTTCATTACCATTACAACTTCTAACACGAGATATTTTCTTAAATTTTAGGGACCAAATGGAACTACGTTACCAGGACCGTGTGATAGCCCAATTGTAAGTTCATATACCCAATCATATGATTATTTTCTACTAACACATGAGAAAAAATTACTTAGGCATCACATTCTAATGATTTTAGGCTATAAGGTTCTTCATCAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGTAAGAATGTGCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCTAATACGGACGGAATCCACATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGTTAGTAGGTTGAACCAAAAGTAAAAATTAGCTATAATCACTTTGTTAAGAAAATGAATTTTAAATCCTGACTATTTTTTATTATGTGTAGGGGATGATTGTATATCAATTGGGTCTGGTTCATATGATTTAGATATTAGAAACATCACTTGTGGACCAAGTCATGGAATTAGGTATTGTTTAATTTAGTTGTTTTAAAAACATAATTGTTGTTGCCAGCTTGATTATTAGTTCAACTAGTTAAGACATTGCAACTTTTTCCTGTTTGATATTTTGGTCTAGTATTGGAAGTCTCGGTGTACGAAACTCAAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCAGTGATCAAAGACTCAGACAATGGAGTTAGGATAAAGACATGGCAAGGTGGATATGGAGCAGTACGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGACCAATATTACTGTCTAACCAAAGATTGCACCAACCAAACTTCAGCTGTCTCAGTATCAGACATTCAATACACAAACATTAAAGGAACATACGACATTCGGAGCCCACCTATGCACTTCGCCTGCAGTGACTCTGTCCCGTGTACCAATCTAACGCTGTCAGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACGCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTCCCTTCTTCTATCAACTTAAACAATGATAATGAGTATTGTTAG

mRNA sequence

ATGTCTAGTTTCGTGTTGTCATTTGCAATTTGGTTATCTTTTATTTCTTGTCTTCTTTCTTCTTCTTCTCAAGCTAGATTGCATCATCACACCAAACAAAAACATTCTCACCACCATAAACATAGCTACATTTCGCACCCAAATTCTCCTTCTCCTTCTCCTTCTAATTCACCCGACGACGGAGATTACGTTAACGAGACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGTGTTGGAGATGACACGGGGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAAAGTGAAGACTCTGCCATCATGCTAGTTCCCTATGGCTTCTCATTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCACGGTAATCTCATGTTACGAGTCGACGGCACGTTGATGCCACCGGATGGCCCCGATGCATGGCCGAAGAATGCTAGTCGCCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGAAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAAGGACCAAATGGAACTACGTTACCAGGACCGTGTGATAGCCCAATTGCTATAAGGTTCTTCATCAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGTAAGAATGTGCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCTAATACGGACGGAATCCACATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTATATCAATTGGGTCTGGTTCATATGATTTAGATATTAGAAACATCACTTGTGGACCAAGTCATGGAATTAGTATTGGAAGTCTCGGTGTACGAAACTCAAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCAGTGATCAAAGACTCAGACAATGGAGTTAGGATAAAGACATGGCAAGGTGGATATGGAGCAGTACGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGACCAATATTACTGTCTAACCAAAGATTGCACCAACCAAACTTCAGCTGTCTCAGTATCAGACATTCAATACACAAACATTAAAGGAACATACGACATTCGGAGCCCACCTATGCACTTCGCCTGCAGTGACTCTGTCCCGTGTACCAATCTAACGCTGTCAGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACGCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTCCCTTCTTCTATCAACTTAAACAATGATAATGAGTATTGTTAG

Coding sequence (CDS)

ATGTCTAGTTTCGTGTTGTCATTTGCAATTTGGTTATCTTTTATTTCTTGTCTTCTTTCTTCTTCTTCTCAAGCTAGATTGCATCATCACACCAAACAAAAACATTCTCACCACCATAAACATAGCTACATTTCGCACCCAAATTCTCCTTCTCCTTCTCCTTCTAATTCACCCGACGACGGAGATTACGTTAACGAGACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGTGTTGGAGATGACACGGGGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAAAGTGAAGACTCTGCCATCATGCTAGTTCCCTATGGCTTCTCATTTATGATTCAATCAACAATCTTTACTGGCCCTTGCCACGGTAATCTCATGTTACGAGTCGACGGCACGTTGATGCCACCGGATGGCCCCGATGCATGGCCGAAGAATGCTAGTCGCCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGAAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAAGGACCAAATGGAACTACGTTACCAGGACCGTGTGATAGCCCAATTGCTATAAGGTTCTTCATCAGCTCCAATTTGACTGTCCAAGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGTAAGAATGTGCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCTAATACGGACGGAATCCACATCGAAAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTATATCAATTGGGTCTGGTTCATATGATTTAGATATTAGAAACATCACTTGTGGACCAAGTCATGGAATTAGTATTGGAAGTCTCGGTGTACGAAACTCAAGAGCTTGTGTTACAAACATAACGGTCAGGGACTCAGTGATCAAAGACTCAGACAATGGAGTTAGGATAAAGACATGGCAAGGTGGATATGGAGCAGTACGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGACCAATATTACTGTCTAACCAAAGATTGCACCAACCAAACTTCAGCTGTCTCAGTATCAGACATTCAATACACAAACATTAAAGGAACATACGACATTCGGAGCCCACCTATGCACTTCGCCTGCAGTGACTCTGTCCCGTGTACCAATCTAACGCTGTCAGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACGCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTCCCTTCTTCTATCAACTTAAACAATGATAATGAGTATTGTTAG

Protein sequence

MSSFVLSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC
Homology
BLAST of HG10023050 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 938.3 bits (2424), Expect = 2.5e-269
Identity = 449/469 (95.74%), Postives = 459/469 (97.87%), Query Frame = 0

Query: 1   MSSFVLSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPN--SPSPSPSNS- 60
           MSSFVLSFAIW SFISCLLSSSSQAR HHHTKQKHSHHHKHSYISHP   SPSPSPS+S 
Sbjct: 1   MSSFVLSFAIWFSFISCLLSSSSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPSDSP 60

Query: 61  PDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQS 120
           PDDGDY+NETNIFDVRAFGAIG+GV DDT AFKMAWDTACQS+DSAIMLVPYG+SFMIQS
Sbjct: 61  PDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYSFMIQS 120

Query: 121 TIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQK 180
           TIFTGPCHG+LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQK
Sbjct: 121 TIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQK 180

Query: 181 WWDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHI 240
           WWDLPCKPHKG NGTTLPGPCDSPIAIRFF+SSNLTVQGLKITNSPQFHFRFDGCKNVHI
Sbjct: 181 WWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHI 240

Query: 241 DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHG 300
           DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDC+SIGSGSYDLDIRNITCGPSHG
Sbjct: 241 DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGPSHG 300

Query: 301 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP 360
           ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP
Sbjct: 301 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP 360

Query: 361 IIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIE 420
           IIIDQ+YCLTKDC NQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIE
Sbjct: 361 IIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIE 420

Query: 421 LLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LLP+QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC
Sbjct: 421 LLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of HG10023050 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 926.8 bits (2394), Expect = 7.5e-266
Identity = 444/468 (94.87%), Postives = 455/468 (97.22%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSHHH+HSYISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DGDY+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DSA+MLVPYGFSFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of HG10023050 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 925.2 bits (2390), Expect = 2.2e-265
Identity = 443/468 (94.66%), Postives = 454/468 (97.01%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSHHH+H YISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DGDY+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DSA+MLVPYGFSFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of HG10023050 vs. NCBI nr
Match: XP_004135975.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein Csa_016395 [Cucumis sativus])

HSP 1 Score: 915.2 bits (2364), Expect = 2.2e-262
Identity = 439/468 (93.80%), Postives = 451/468 (96.37%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSH H+HSYISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DG Y+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DS +MLVPYGFSFMIQST
Sbjct: 61  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+N NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of HG10023050 vs. NCBI nr
Match: XP_022992012.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 891.3 bits (2302), Expect = 3.5e-255
Identity = 417/466 (89.48%), Postives = 443/466 (95.06%), Query Frame = 0

Query: 1   MSSFVLSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPDD 60
           MS+FVLS + W++F+ CLL  SSQAR HHH+KQKHSHHHK SYISHP +PSPSP     D
Sbjct: 1   MSNFVLSLSFWVTFLCCLL-CSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPG----D 60

Query: 61  GDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIF 120
           GDY+NETNIFDVR FGA+GDGV DDT AFKMAWDTACQS+D+A++LVPYG+SFMIQSTIF
Sbjct: 61  GDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIF 120

Query: 121 TGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180
           TGPCHG+LML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWD
Sbjct: 121 TGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWD 180

Query: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240
           LPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTVQGLK TNSPQFHFRFDGCKNVHI+SI
Sbjct: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESI 240

Query: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISI 300
           HIKSPGLSPNTDGIHIENTN V IYNS+IYNGDDC+SIGSGSYD+DIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360
           GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360

Query: 361 DQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLP 420
           DQ+YCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLP
Sbjct: 361 DQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLP 420

Query: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI LNNDNEYC
Sbjct: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of HG10023050 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 6.1e-162
Identity = 257/420 (61.19%), Postives = 323/420 (76.90%), Query Frame = 0

Query: 47  PNSPSPSPSNSPDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIML 106
           P+ P PSP  +P D D      +FDV +FGA+GDG  DDT AF+ AW  AC  E S ++L
Sbjct: 60  PSDPYPSPDPAPGDSD---SGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE-SGVVL 119

Query: 107 VPYGFSFMIQSTIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLE 166
            P G  F I STIF+GPC   L+ ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  
Sbjct: 120 APEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFS 179

Query: 167 GGGVIDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFH 226
           G G ++G GQKWWDLPCKPH+GP+G++  GPC SP  IRFF+S+N+ V+GL+I NSPQFH
Sbjct: 180 GKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFH 239

Query: 227 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLD 286
            +FDGC+ V I+ I I SP LSPNTDGIH+ NT  V IYNSV+ NGDDCISIG+G  D+D
Sbjct: 240 MKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVD 299

Query: 287 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITF 346
           I+ +TCGPSHGISIGSLGV NS+ACV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F
Sbjct: 300 IQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLF 359

Query: 347 SNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSV 406
            NI M+NV N II+DQYYC +KDC N+TSAV V D++Y NIKGTYD+RSPP+HFACSD+V
Sbjct: 360 ENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTV 419

Query: 407 PCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 466
            CTN+T+SE+ELLP +G++V DPFCWNAYG  +TLTIPP+ CL +G P      + N  C
Sbjct: 420 ACTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of HG10023050 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.2e-93
Identity = 171/385 (44.42%), Postives = 233/385 (60.52%), Query Frame = 0

Query: 69  IFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGPCHGNL 128
           +  V  FGA G+GV DDT AF  AW TAC S+    +LVP  ++ +++    +GPC   L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 129 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 188
            L++ GT++ P+ PD W +  +RR+WL F+ +  +++EGGG ++G GQ+WW+  CK H  
Sbjct: 112 TLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNH 171

Query: 189 PNGTTLPGPC-DSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGL 248
            N      PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + + +P  
Sbjct: 172 SN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 231

Query: 249 SPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISIGSLGVRN 308
           SPNTDGIHI  + G+ I N+ +  GDDCISI   S  + I NI CGP HGISIGSLG   
Sbjct: 232 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSK 291

Query: 309 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYC-L 368
           S   V +ITV  ++I D+ NGVRIKTWQGG G V  I F NI M+NV NPIIIDQYYC  
Sbjct: 292 SWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDS 351

Query: 369 TKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIV 428
            K C NQTSA+S+ +I + +++GT       +  +CSDS PC N+ L +I+L P+ GD  
Sbjct: 352 RKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGF 411

Query: 429 LDPFCWNAYGDLQTLTIPPVSCLQE 452
            + FCW AYG       PP  CL +
Sbjct: 412 TESFCWEAYGSSSGQVYPP-PCLSD 426

BLAST of HG10023050 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.9e-83
Identity = 158/359 (44.01%), Postives = 216/359 (60.17%), Query Frame = 0

Query: 68  NIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGPCHGN 127
           NIF+V  +GA+GDG  D T AF  AW  AC+ + SA++LVP    F++ +  F GPC  +
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACK-KPSAMLLVPGNKKFVVNNLFFNGPCQPH 116

Query: 128 LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 187
              +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   K
Sbjct: 117 FTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC---K 176

Query: 188 GPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGL 247
             NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P  
Sbjct: 177 WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRD 236

Query: 248 SPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISIGSLGVRN 307
           SPNTDGI I  +    +  + I  GDDC++IG+GS ++ I ++ CGP HGISIGSLG  N
Sbjct: 237 SPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGREN 296

Query: 308 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYCLT 367
           SRA V+ + V  +   D+ NG+RIKTWQGG G    I + N+ M N  NPI+I+Q+YC +
Sbjct: 297 SRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTS 356

Query: 368 KD-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 426
              C NQ SAV + D+ Y NI+GT    +  +   CSDS+PC ++ LS+I L    G I
Sbjct: 357 ASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of HG10023050 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.4e-81
Identity = 157/356 (44.10%), Postives = 217/356 (60.96%), Query Frame = 0

Query: 69  IFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGPCHGNL 128
           +F+V  +GA+GDG  D T AF   W+ AC+ + SA++LVP    F + + +F GPC  +L
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACK-KASAVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 129 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 188
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 189 PNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 248
            NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 249 PNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISIGSLGVRNS 308
           PNTDGI I  +    I   VI  GDDCI+IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 309 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYCLTK 368
           RA V+++ V  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+Q+YC + 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 369 D-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQG 424
             C NQ SAV +  + Y NI GT    +  +   CSDSVPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of HG10023050 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.4e-80
Identity = 153/358 (42.74%), Postives = 217/358 (60.61%), Query Frame = 0

Query: 69  IFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGPCHGNL 128
           +F+V  +GA+GDG  D T AF+  W+ AC ++ SA+ LVP    F++ + +F GPC  + 
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAAC-NKLSAVFLVPANKKFVVNNLVFYGPCQPHF 118

Query: 129 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 188
             +VDGT+     P  W KN+  + W+ F R+   +L G GVIDG+G +WW   CK    
Sbjct: 119 SFKVDGTIAAYPDPAKW-KNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKT--- 178

Query: 189 PNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 248
            NG T+      P AI+   S ++TV+ L +TNSP+FH  F  C  V I  I IK+P  S
Sbjct: 179 INGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRDS 238

Query: 249 PNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISIGSLGVRNS 308
           PNTDGI I  +   EI    I  GDDC+++G+GS ++ I+++TCGP HG+SIGSLG  NS
Sbjct: 239 PNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGNS 298

Query: 309 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYCLT- 368
           R+ V+ + +  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+Q+YC + 
Sbjct: 299 RSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTSA 358

Query: 369 KDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 426
             C NQ SAV + D+ + NI GT    +  +   CSDSVPC+N+ LS + L    G +
Sbjct: 359 AACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

BLAST of HG10023050 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 3.6e-266
Identity = 444/468 (94.87%), Postives = 455/468 (97.22%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSHHH+HSYISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DGDY+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DSA+MLVPYGFSFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of HG10023050 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.1e-265
Identity = 443/468 (94.66%), Postives = 454/468 (97.01%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSHHH+H YISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DGDY+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DSA+MLVPYGFSFMIQST
Sbjct: 61  DGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of HG10023050 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.1e-262
Identity = 439/468 (93.80%), Postives = 451/468 (96.37%), Query Frame = 0

Query: 1   MSSFV-LSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPD 60
           MSSFV LSFAIWLSF+SCLL SSSQAR HHHTKQKHSH H+HSYISHP S SPSPS+SPD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL-SSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPD 60

Query: 61  DGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSE-DSAIMLVPYGFSFMIQST 120
           DG Y+N+TNIFDVRAFGAIGDG+ DDT AFKMAWDTACQSE DS +MLVPYGFSFMIQST
Sbjct: 61  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 120

Query: 121 IFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180
           IFTGPCHG LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 121 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 180

Query: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHID 240
           WDLPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTV GLKITNSPQFHFRFDGCK VHID
Sbjct: 181 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 240

Query: 241 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGI 300
           SIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDC+SIGSGSYDLDIRNITCGPSHGI
Sbjct: 241 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 300

Query: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360
           SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI
Sbjct: 301 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 360

Query: 361 IIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420
           IIDQ+YCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL
Sbjct: 361 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 420

Query: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+N NNDNEYC
Sbjct: 421 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of HG10023050 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 417/466 (89.48%), Postives = 443/466 (95.06%), Query Frame = 0

Query: 1   MSSFVLSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPDD 60
           MS+FVLS + W++F+ CLL  SSQAR HHH+KQKHSHHHK SYISHP +PSPSP     D
Sbjct: 1   MSNFVLSLSFWVTFLCCLL-CSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPG----D 60

Query: 61  GDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIF 120
           GDY+NETNIFDVR FGA+GDGV DDT AFKMAWDTACQS+D+A++LVPYG+SFMIQSTIF
Sbjct: 61  GDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIF 120

Query: 121 TGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180
           TGPCHG+LML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWD
Sbjct: 121 TGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWD 180

Query: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240
           LPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTVQGLK TNSPQFHFRFDGCKNVHI+SI
Sbjct: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESI 240

Query: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISI 300
           HIKSPGLSPNTDGIHIENTN V IYNS+IYNGDDC+SIGSGSYD+DIRNITCGPSHGISI
Sbjct: 241 HIKSPGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360
           GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360

Query: 361 DQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLP 420
           DQ+YCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLP
Sbjct: 361 DQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLP 420

Query: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI LNNDNEYC
Sbjct: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of HG10023050 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.9e-255
Identity = 417/466 (89.48%), Postives = 442/466 (94.85%), Query Frame = 0

Query: 1   MSSFVLSFAIWLSFISCLLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPDD 60
           MS+FVLS + W++F+ CLL  SSQAR HHH+KQKHSHHHK SYISHP +PSPSP     D
Sbjct: 1   MSNFVLSLSFWVTFLCCLL-CSSQARWHHHSKQKHSHHHKRSYISHPPTPSPSPG----D 60

Query: 61  GDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIF 120
           GDY+NETNIFDVR FGA+GDGV DDT AFKMAWD ACQS+D+A++LVPYG+SFMIQSTIF
Sbjct: 61  GDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIF 120

Query: 121 TGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWD 180
           TGPCHG+LML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWD
Sbjct: 121 TGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWD 180

Query: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 240
           LPCKPHKGPNGTTLPGPCDSPIAIRFF+SSNLTVQGLK TNSPQFHFRFDGCKNVHI+SI
Sbjct: 181 LPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESI 240

Query: 241 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHGISI 300
           HIKSPG SPNTDGIHIENTNGV IYNSVIYNGDDC+SIGSGSYD+DIRNITCGPSHGISI
Sbjct: 241 HIKSPGFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISI 300

Query: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360
           GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII
Sbjct: 301 GSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIII 360

Query: 361 DQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLP 420
           DQ+YCLTKDCTNQTSAVS+SDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTLSEIELLP
Sbjct: 361 DQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLP 420

Query: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 467
           AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI LNNDNEYC
Sbjct: 421 AQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of HG10023050 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 642.5 bits (1656), Expect = 2.6e-184
Identity = 307/469 (65.46%), Postives = 374/469 (79.74%), Query Frame = 0

Query: 12  LSFISCLLS--SSSQARLHHH-TKQKHSHHHKH--------SYISHPNSPSPSPSN---- 71
           L F++ +L+  S S+AR HHH  K KH++H+ H        S IS P +P P P +    
Sbjct: 12  LGFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAP 71

Query: 72  ----SPDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDS--AIMLVPYG 131
               SP D    +   I++VR +GA+GDG  DDT AFK AWD++C +E++  +++LVPYG
Sbjct: 72  SLPPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYG 131

Query: 132 FSFMIQSTIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGV 191
           ++FMIQSTIFTGPC      +VDGT++ PDGP++WP N S+RQWLVFYR++GM+L+G GV
Sbjct: 132 YTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGV 191

Query: 192 IDGRGQKWWDLPCKPHKGPNGTTL-PGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRF 251
           IDGRGQKWWDLPCKPH+  N + +  GPCDSPIA+RFF+SSNL V+GL+I NSPQFHFRF
Sbjct: 192 IDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRF 251

Query: 252 DGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRN 311
           DGC+ VH++S+HI +P LSPNTDGIHIEN+N V IYNS+I NGDDC+SIGSGSYD+DIRN
Sbjct: 252 DGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRN 311

Query: 312 ITCGP-SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSN 371
           +TCGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+N
Sbjct: 312 LTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNN 371

Query: 372 IHMDNVRNPIIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPC 431
           IH+D+VRNPIIIDQYYC+TKDC N+TSAV VSDI Y  IKGTYDIRSPPMHF CSD+VPC
Sbjct: 372 IHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPC 431

Query: 432 TNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI 458
           TNLTLS+IELLPA+G+IVLDPFCWNAYG  + L+IPPV CL    P  +
Sbjct: 432 TNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of HG10023050 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 586.3 bits (1510), Expect = 2.2e-167
Identity = 279/445 (62.70%), Postives = 343/445 (77.08%), Query Frame = 0

Query: 18  LLSSSSQARLHHHTKQKHSHHHKHSYISHPNSPSPSPSNSPDDGDYVNETNIFDVRAFGA 77
           LLS  ++ARLHH   Q  S         +PN PS SPS S D    ++   ++DVR +GA
Sbjct: 23  LLSYGTEARLHHQASQPPSPS------PNPNDPSKSPSRSQD----LDHEVVYDVRKYGA 82

Query: 78  IGDGVGDDTGAFKMAWDTAC---QSEDSAIMLVPYGFSFMIQSTIFTGPCHGNLMLRVDG 137
           +G+GV DDT +FK AWD+AC   ++  ++++ VPYGF+FMI+STIFTGPC      +VDG
Sbjct: 83  VGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDG 142

Query: 138 TLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH-KGPNGTT 197
           T++P DGP +WP + ++RQWL FYRI+GM+L+G GVIDGRGQ WWDLPCKPH +  N T 
Sbjct: 143 TIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTK 202

Query: 198 LPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDG 257
           L GPC+SP A+RFF+SSN+ V+GL I NSPQ H + DGC  VHI+S+ I SP  SPNTDG
Sbjct: 203 LAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDG 262

Query: 258 IHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGP-SHGISIGSLGVRNSRACV 317
           IHIEN+N VEIYNSVI NGDDC+SIG G+YD+DIRNITCGP  HGISIGSLG +NS ACV
Sbjct: 263 IHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACV 322

Query: 318 TNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQYYCLTKDCTN 377
           +N+TVRDS IK S+NGVRIKTWQGG G+V G+TF NIH+D VRNPIIIDQYYC TK C N
Sbjct: 323 SNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCAN 382

Query: 378 QTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCW 437
           +TSAV V+DI Y +IKGTYDIRSPPMHF CS++VPCTNLTLS IELLP++ DIV+ PFCW
Sbjct: 383 KTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCW 442

Query: 438 NAYGDLQTLTIPPVSCLQEGLPSSI 458
           NAYG     ++P +SCL+    +S+
Sbjct: 443 NAYGITDEFSVPLISCLKSNPSTSL 457

BLAST of HG10023050 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 572.0 bits (1473), Expect = 4.3e-163
Identity = 257/420 (61.19%), Postives = 323/420 (76.90%), Query Frame = 0

Query: 47  PNSPSPSPSNSPDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQSEDSAIML 106
           P+ P PSP  +P D D      +FDV +FGA+GDG  DDT AF+ AW  AC  E S ++L
Sbjct: 60  PSDPYPSPDPAPGDSD---SGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE-SGVVL 119

Query: 107 VPYGFSFMIQSTIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLE 166
            P G  F I STIF+GPC   L+ ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  
Sbjct: 120 APEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFS 179

Query: 167 GGGVIDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFH 226
           G G ++G GQKWWDLPCKPH+GP+G++  GPC SP  IRFF+S+N+ V+GL+I NSPQFH
Sbjct: 180 GKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFH 239

Query: 227 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLD 286
            +FDGC+ V I+ I I SP LSPNTDGIH+ NT  V IYNSV+ NGDDCISIG+G  D+D
Sbjct: 240 MKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVD 299

Query: 287 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITF 346
           I+ +TCGPSHGISIGSLGV NS+ACV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F
Sbjct: 300 IQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLF 359

Query: 347 SNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSV 406
            NI M+NV N II+DQYYC +KDC N+TSAV V D++Y NIKGTYD+RSPP+HFACSD+V
Sbjct: 360 ENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTV 419

Query: 407 PCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 466
            CTN+T+SE+ELLP +G++V DPFCWNAYG  +TLTIPP+ CL +G P      + N  C
Sbjct: 420 ACTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of HG10023050 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.0e-160
Identity = 258/408 (63.24%), Postives = 326/408 (79.90%), Query Frame = 0

Query: 49  SPSPSPSNSPDDGDYVNETNIFDVRAFGAIGDGVGDDTGAFKMAWDTACQS--EDSAIML 108
           SPSP+P+ + +D D +  T +FDV +FGAIGD   DDT AFKMAWD AC S    SA++L
Sbjct: 28  SPSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLL 87

Query: 109 VPYGFSFMIQSTIFTGPCHGNLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLE 168
           VPY F F+++ T F GPC  NL+L++DG ++ PDGP +WP N  +RQW++FYR++G+S++
Sbjct: 88  VPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLSIQ 147

Query: 169 GGGVIDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFH 228
           G GVI+GRGQKWW+LPCKPHKG NGTT  GPCDSP+AIR F SS + +QG+   NS QFH
Sbjct: 148 GSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFH 207

Query: 229 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLD 288
            RFD C +V +DS+ IK+P  SPNTDGIHIENT+ V+I NS+I NGDDCISIG+G +++D
Sbjct: 208 VRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVD 267

Query: 289 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITF 348
           I+N+TCGPSHGISIGSLGV NS+A V+NITV +S I +SDNGVRIKTWQGG G+V  I F
Sbjct: 268 IKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVF 327

Query: 349 SNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSV 408
           SNI M NVRNPI+IDQYYC T +C NQTSAV +SD+ Y NIKGTYD+RSPP+HF CSDSV
Sbjct: 328 SNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSV 387

Query: 409 PCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLP 455
           PCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL +  P
Sbjct: 388 PCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of HG10023050 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 376.7 bits (966), Expect = 2.7e-104
Identity = 199/530 (37.55%), Postives = 299/530 (56.42%), Query Frame = 0

Query: 5   VLSFAIWLSFISCLLSSSSQARLHHH-----------TKQKHSHHHKHSYISHPNSPSPS 64
           +++  +W + +   ++   +   HHH           +K+  SH + H ++SH + P P 
Sbjct: 17  LVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMSHKSKPKPK 76

Query: 65  PSNSP---DDG---------------------------DYVNETNIFDVRAFGAIGDGVG 124
               P   +DG                             V ++  F+V  FGA GDG+ 
Sbjct: 77  MKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVLDFGAKGDGMS 136

Query: 125 DDTGAFKMAWDTACQSEDSAIMLVPYGFSFMIQSTIFTGP-CHGNLMLRVDGTLMPPDGP 184
           DDT AF+ AW +AC+ E S  M++P  + F++    F+GP C  N++ +++G ++ P   
Sbjct: 137 DDTQAFEAAWASACKVEAST-MIIPPDYIFLVGPISFSGPYCQANIVFQLEGMIVAPTDT 196

Query: 185 DAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW----------------------- 244
           ++W        W+ F ++ G++++G GVIDGRG  WW                       
Sbjct: 197 ESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNS 256

Query: 245 --DLPCKPHKGPNGTTLPGPCDSPIAIRFFISSNLTVQGLKITNSPQFHFRFDGCKNVHI 304
             + P  P +      +P     P A+RF+ S ++TV G+ I NSPQ H +FD C  V +
Sbjct: 257 VQERPPMPIRSELNWRMPS--IKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLV 316

Query: 305 DSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCISIGSGSYDLDIRNITCGPSHG 364
             +++ SPG SPNTDGIH++NT  V I+ + +  GDDCISI +G  ++ + N+ CGP HG
Sbjct: 317 HDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNCGPGHG 376

Query: 365 ISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNP 424
           ISIGSLG  +++ACV+NITVRD V+ ++  GVRIKTWQGG G+V+GI FSNI +  V+ P
Sbjct: 377 ISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLP 436

Query: 425 IIIDQYYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIE 467
           I+IDQ+YC    C N TSAVSV  + Y  I+GTY ++  P+HFACSDS PC ++ LS IE
Sbjct: 437 IVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCIDVQLSGIE 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899064.12.5e-26995.74polygalacturonase At1g48100-like [Benincasa hispida][more]
TYJ96898.17.5e-26694.87polygalacturonase [Cucumis melo var. makuwa][more]
XP_008461394.12.2e-26594.66PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
XP_004135975.12.2e-26293.80polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein C... [more]
XP_022992012.13.5e-25589.48polygalacturonase At1g48100 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q949Z16.1e-16261.19Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ51.2e-9344.42Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432121.9e-8344.01Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E72.4e-8144.10Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY193.4e-8042.74Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BFC73.6e-26694.87Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00... [more]
A0A1S3CFX81.1e-26594.66polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV... [more]
A0A0A0KBA71.1e-26293.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1[more]
A0A6J1JNI51.7e-25589.48polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=... [more]
A0A6J1GP582.9e-25589.48polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G02460.12.6e-18465.46Pectin lyase-like superfamily protein [more]
AT4G01890.12.2e-16762.70Pectin lyase-like superfamily protein [more]
AT1G48100.14.3e-16361.19Pectin lyase-like superfamily protein [more]
AT1G56710.12.0e-16063.24Pectin lyase-like superfamily protein [more]
AT1G60590.12.7e-10437.55Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 259..280
e-value: 51.0
score: 12.7
coord: 341..362
e-value: 710.0
score: 8.3
coord: 232..258
e-value: 94.0
score: 11.9
coord: 312..333
e-value: 1.1
score: 18.2
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 97..418
e-value: 1.8E-89
score: 300.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 289..302
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 63..456
e-value: 1.7E-120
score: 404.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..44
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 22..455
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 22..455
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 67..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023050.1HG10023050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity