HG10023042 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10023042
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptiongibberellin 2-beta-dioxygenase 2-like
LocationChr05: 30725048 .. 30727865 (+)
RNA-Seq ExpressionHG10023042
SyntenyHG10023042
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTGTCCCACACCCATGAGGACCAAGAAGACCAAAGCTGTCGGAATTCCCACCATAGATCTCTCTCAAAGCAGAGCAACAACTTCCAAATCCATTATTGACGCTTCTCAAGACTTTGGCTTCTTCAAGCTCATCAACCATGGAGTTTCTCTTGATGCCATTGCTTCACTTGAATCCCATGCCTTTGATTTTTTTCAAAAAACCGCCCCCGAGAAGCACCGCGCCGGTCCCCCCAACCCCTTCGGTTACGGCTGCAAATCTATCGGCCCCAATGGCGACTTGGGCGACCTCGAGTACCTTCTTCTTCGCTCCGACCCTACTTCCGTTGTTGAAAGCTCCACCTCCATCTCTAACGACCCTCCTACTTTCAGGTTATTTTTATATTTTAAAAAGTTTCTAATAAGTGGTTGAACTTTTAGAAGTGTGTCTACTTTTATTTTATAAAAAAACATACTTTCTCATTATCGCTAAAAATTTGACAATTATTTATCTTTTGAAAAACAATGAAAAAAAGTTATTTTATTCTCTCGTTTTCTGCATTATGGTTAAAAACTTTAATGATAGTGAATAAGAATAGTTTTTCAAATTTTTAATAGGTGTCTAAATTTTTTTAGATTAATTGGTTTTTGGCATTCAAGAATGCTGATGTATTATAGCTAGATATAAAATTGAAAAATTGAAAAATTTATTATACATTTTTTTAAAAAAAATTTTAAAAGTAGAAAAAATTGGAAAACTATTTACATAAAATAGCAAAATTTTTAGATAGTTGTGATAGACGCGGATAGAAGTTTATCAGAGTCTATCAGTGATAGAAATGATATAAGTTTATTACTGGTAGATGTTGATAGAAGTATATCAATGCCTATCCGTGTTTTTTTTTGCTATTTTCTATAAATAGTTTGACATTTTTTTATATGAGAAAAAATTCTTTTTTTTTTTTTTTAAATATAAAGATCATTAAATACATACAATATTAAAAATTCATAGACGTTCTAAAAACTATTTGAAGTAGAGGAACCGACCATGGATTTCCAAGATCAAACATATAATTTAACCATTAATATTCATTTTCTTGTTCAAGAACAAAATATTAAAGAAGACAACTTTTCTTCTGTTTTTCTTTTTTTTTTTTTTCCTCTTCTCTTCTTTGATTATATAATTTCCATTGGAGAATGCATTTTCATTTATGGGTCAAACCACTTGGCTTTAAAAGCTGCAAAGAATCATATTTTTAATGTCAAAACGACATTTTTCTTTCTTTATGGAAAAAAAGAGAACAATATTTTATCTAATGACACCAATATTTTTTGAGAAATATAATATACATTGGGTCATTATCAATGGGAAAACATATAATATTATTTAAAAATATTAATATACATATTATAAAAAATGGAGGAAGAGACAACTATTTACTCTAGATATGATATAATTTAGTCGTCAATATTTTATATTTTTTGTAAAAAGTTTTGCATACGTTTTGACAATGATTTTGTATTTAATAATTGTGCTTCAAAAGTGAATTTTGTTTATATGTAGGGTTTTTTTTTTTTTATTATTTTTTTTAAATATCTTAAAGTCAAGTTGTAGCTAAATTTTGTAAAAAACCAAAAAGAGTTGTTTTGTTGGAAAAATTTTATTAAAAAAAAAAAAAAAATTGTTTGCAAGGGGGGTTTTCCAAATAGTTTCCTTGATTTGTTTCTTAAATTTATTTTAAAGAATAGTTTGTTATAAAGATCAAAATTGCAAGATTATGGTATTTACCAATAATTAATTCTATTCATTTTGGATATATTTGAACAAACATGCAAGGTACACAAAATGACTTTTTGATATTTAAACGAAAAATGTCAATTAAAAAAATACAATTAATGTACATTCCATAAGAAGGTTATAAACATCAATATACTTGTTAATAAATATTTCACAATATTTGAAAAAATGAGTTACTAACACTACTAATTACTACTGATAATTCTTTAAATATTTTGTTGCTATCTTTATGAAAAATTGAAAATTTGATATTTTAATTTGTTACAGCTATGCCGTGAATGAGTATGTTAAAAACGTGAGGGAATTAGGATGTGAGATTCTTGATTTAGTGGGTGAAGGTCTATGGTTACCAAACAAGTCTGTGTTCAGTAATCTCATCAGAGACGTCCACAATGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCAAGCACACTGAAAATTGGGAACCTTCACCAAAATCTCACCCTTCAAATAACAGAATTGGATTTGGGGAGCATTCTGATCCTCAGATCTTAACCATCTTGAGATCCAACGACGTCGATGGCCTGCAAATCTCCTTGCAAGACGGGATCTGGGTGCCTGTTTGCCCTGACCCGTCTGCATTCTACGTCTTGATTGGTGATGCTTTTAAGGTAAATATATGTCAATTCATTCATTTATTATGTATTTGAGATTAAATTATGATTGTAAACCCTAATGTCATTGATTTAGAGATGTCGAGTTTTAAAACAGGTTGAAGCGATAAATCGGGTATTAAGATTTGTTGTGTTTGTTTTTGAACGCAGGCAATGACAAATGGGAGATTTCTAAGTGTAAGGCATAGAGCAATGGCAAACAACTCAAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCTTGGATTGCACCTTTATCAGAGATGATGCTACCAAATGAACAATCTTTATATAAGCCTTTCACTTGGGCTCAATATAAGAAAGCTGCTTATTCTCTTAGATTAGCTGATTCTCGTCTTGATCTCTTCAAATTAACTTCCTAA

mRNA sequence

ATGCCTTGTCCCACACCCATGAGGACCAAGAAGACCAAAGCTGTCGGAATTCCCACCATAGATCTCTCTCAAAGCAGAGCAACAACTTCCAAATCCATTATTGACGCTTCTCAAGACTTTGGCTTCTTCAAGCTCATCAACCATGGAGTTTCTCTTGATGCCATTGCTTCACTTGAATCCCATGCCTTTGATTTTTTTCAAAAAACCGCCCCCGAGAAGCACCGCGCCGGTCCCCCCAACCCCTTCGGTTACGGCTGCAAATCTATCGGCCCCAATGGCGACTTGGGCGACCTCGAGTACCTTCTTCTTCGCTCCGACCCTACTTCCGTTGTTGAAAGCTCCACCTCCATCTCTAACGACCCTCCTACTTTCAGCTATGCCGTGAATGAGTATGTTAAAAACGTGAGGGAATTAGGATGTGAGATTCTTGATTTAGTGGGTGAAGGTCTATGGTTACCAAACAAGTCTGTGTTCAGTAATCTCATCAGAGACGTCCACAATGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCAAGCACACTGAAAATTGGGAACCTTCACCAAAATCTCACCCTTCAAATAACAGAATTGGATTTGGGGAGCATTCTGATCCTCAGATCTTAACCATCTTGAGATCCAACGACGTCGATGGCCTGCAAATCTCCTTGCAAGACGGGATCTGGGTGCCTGTTTGCCCTGACCCGTCTGCATTCTACGTCTTGATTGGTGATGCTTTTAAGGCAATGACAAATGGGAGATTTCTAAGTGTAAGGCATAGAGCAATGGCAAACAACTCAAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCTTGGATTGCACCTTTATCAGAGATGATGCTACCAAATGAACAATCTTTATATAAGCCTTTCACTTGGGCTCAATATAAGAAAGCTGCTTATTCTCTTAGATTAGCTGATTCTCGTCTTGATCTCTTCAAATTAACTTCCTAA

Coding sequence (CDS)

ATGCCTTGTCCCACACCCATGAGGACCAAGAAGACCAAAGCTGTCGGAATTCCCACCATAGATCTCTCTCAAAGCAGAGCAACAACTTCCAAATCCATTATTGACGCTTCTCAAGACTTTGGCTTCTTCAAGCTCATCAACCATGGAGTTTCTCTTGATGCCATTGCTTCACTTGAATCCCATGCCTTTGATTTTTTTCAAAAAACCGCCCCCGAGAAGCACCGCGCCGGTCCCCCCAACCCCTTCGGTTACGGCTGCAAATCTATCGGCCCCAATGGCGACTTGGGCGACCTCGAGTACCTTCTTCTTCGCTCCGACCCTACTTCCGTTGTTGAAAGCTCCACCTCCATCTCTAACGACCCTCCTACTTTCAGCTATGCCGTGAATGAGTATGTTAAAAACGTGAGGGAATTAGGATGTGAGATTCTTGATTTAGTGGGTGAAGGTCTATGGTTACCAAACAAGTCTGTGTTCAGTAATCTCATCAGAGACGTCCACAATGATTCAGTTTTGAGGATCAATTACTACCCTGGAGTCAAGCACACTGAAAATTGGGAACCTTCACCAAAATCTCACCCTTCAAATAACAGAATTGGATTTGGGGAGCATTCTGATCCTCAGATCTTAACCATCTTGAGATCCAACGACGTCGATGGCCTGCAAATCTCCTTGCAAGACGGGATCTGGGTGCCTGTTTGCCCTGACCCGTCTGCATTCTACGTCTTGATTGGTGATGCTTTTAAGGCAATGACAAATGGGAGATTTCTAAGTGTAAGGCATAGAGCAATGGCAAACAACTCAAAGAATCCAAGAATGTCAATCATGTACTTTGGGGCACCACCATTGAATGCTTGGATTGCACCTTTATCAGAGATGATGCTACCAAATGAACAATCTTTATATAAGCCTTTCACTTGGGCTCAATATAAGAAAGCTGCTTATTCTCTTAGATTAGCTGATTCTCGTCTTGATCTCTTCAAATTAACTTCCTAA

Protein sequence

MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISNDPPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQYKKAAYSLRLADSRLDLFKLTS
Homology
BLAST of HG10023042 vs. NCBI nr
Match: XP_038896324.1 (gibberellin 2-beta-dioxygenase 2 [Benincasa hispida])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 291/327 (88.99%), Postives = 306/327 (93.58%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT MRTKKTKAVGIPTIDLSQSRA TSKSI++A QDFGFFKLINHGVS DAIA LES
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQSRAITSKSIVEACQDFGFFKLINHGVSDDAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
             FDFF+KTA EKH AGP NPFGYGCKSIGPNGD+GDLEYLLLRSDP+S+++ S SISN 
Sbjct: 61  QGFDFFEKTALEKHCAGPANPFGYGCKSIGPNGDMGDLEYLLLRSDPSSILQCSPSISNH 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFS+ VNEYVK+VR+LGCEILDLV EGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK
Sbjct: 121 PLTFSHVVNEYVKSVRDLGCEILDLVTEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           HTENW+ SPKS  SNNRIGFGEHSDPQILTILRSNDV+GLQISLQDGIW PVCPDPSAFY
Sbjct: 181 HTENWDLSPKSCLSNNRIGFGEHSDPQILTILRSNDVNGLQISLQDGIWAPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGDAF+AMTNGRF+SVRHRAMANNSKN RMSIMYFGAPPLNAWIAPLSEMMLPNEQSL
Sbjct: 241 VLIGDAFQAMTNGRFVSVRHRAMANNSKNARMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. NCBI nr
Match: NP_001292648.1 (gibberellin 2-beta-dioxygenase 2 [Cucumis sativus] >CCD28486.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 583.9 bits (1504), Expect = 8.4e-163
Identity = 285/329 (86.63%), Postives = 306/329 (93.01%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT +RTKKT+AVGIPTIDLSQS+A TSKSI++A QDFGFFKLINHGVS  AIA LES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRS-DP-TSVVESSTSIS 120
             FDFFQK + EKHRAGP NPFGYGCKSIG NGD+GDLEYLLL S DP TS+++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180
           NDP TFS+ VNEYVK VR+LGCEILDL+GEGLWLPNKSVFSNLIRDVH+DSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V+HTEN E S KSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISLQDGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLQDGIWVPVCPDPSA 240

Query: 241 FYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQ 300
           FYVLIGD F+AMTNGRF+SVRHRAMANNSKNPRMSIMYFGAPPLN WIAPLSEMMLPN++
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
           SLYKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 329

BLAST of HG10023042 vs. NCBI nr
Match: KGN45038.1 (hypothetical protein Csa_016303 [Cucumis sativus])

HSP 1 Score: 582.8 bits (1501), Expect = 1.9e-162
Identity = 285/329 (86.63%), Postives = 306/329 (93.01%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT +RTKKT+AVGIPTIDLSQS+A TSKSII+A QDFGFFKLINHGVS  AIA LES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIIEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRS-DP-TSVVESSTSIS 120
             FDFFQK + EKHRAGP NPFGYGCKSIG NGD+GDLEYLLL S DP TS+++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180
           NDP TFS+ VNEYVK VR+LGCEILDL+GEGLWLPNKSVFSNLIRDVH+DSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V+HTEN E S KSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISL+DGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLRDGIWVPVCPDPSA 240

Query: 241 FYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQ 300
           FYVLIGD F+AMTNGRF+SVRHRAMANNSKNPRMSIMYFGAPPLN WIAPLSEMMLPN++
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
           SLYKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 329

BLAST of HG10023042 vs. NCBI nr
Match: XP_022954040.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])

HSP 1 Score: 582.0 bits (1499), Expect = 3.2e-162
Identity = 283/327 (86.54%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT MRTKKTKAVGIPTIDLSQ+R TTSKSI+ A   FGFFKLINHGVS  AI+ LE 
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
           HAFDFF KTAPEKHRAGP  PFGYG K+IGPNGD GDLEYLLLRSDP SV+++S SISND
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFSY VNEYVK+V++LGCEILDLVGEGLWLPNKSVFS+LIRDVH+DSVLRINYYP VK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           HTENWE S KS+PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDG+W PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGDA +AMTNGRF+SVRHRAMANNSK  RMSIMYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. NCBI nr
Match: XP_023547502.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014296.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.5 bits (1495), Expect = 9.3e-162
Identity = 282/327 (86.24%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT MRTKKTK VGIPTIDLSQ+R TTSKSI+ A   FGFFKLINHGVS  AI+ LE 
Sbjct: 1   MPCPTAMRTKKTKVVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
           HAFDFF KTAPEKHRAGP  PFGYG K+IGPNGD GDLEYLLLRSDP SV+++S SISND
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFSY VNEYVK+V++LGCEILDLVGEGLWLPNKSVFS+LIRDVH+DSVLRINYYP VK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           HTENWE S KS+PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDG+W PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGDA +AMTNGRF+SVRHRAMANNSK  RMSIMYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.1e-112
Identity = 196/332 (59.04%), Postives = 254/332 (76.51%), Query Frame = 0

Query: 1   MPCPTPM-RTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLE 60
           +P PT M RTKKTKAVGIPTIDLS  R+  S+ ++ A +++GFFK++NH V  + I+ L+
Sbjct: 3   VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62

Query: 61  SHAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISN 120
               +FF K + EK +AG   PFGYGCK+IGPNGD G+LEYLLL S+P S+ E S +I+ 
Sbjct: 63  EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122

Query: 121 DPP-TFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180
           D P  FS  VN+Y+K V++L CEIL+L  EGLW+P+KS  S +I+D H+DS+LRIN+YP 
Sbjct: 123 DHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRINHYPP 182

Query: 181 VKH--TENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDP 240
           VK    +NW+PS   + +NN IGFGEHSDPQILTILRSN+V GLQIS   G+W+PV PDP
Sbjct: 183 VKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWIPVPPDP 242

Query: 241 SAFYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPN 300
           S FYV++GDA + +TNGRF+SVRHR + N +K PRMS+MYF APPLN  I+PLS+M+  +
Sbjct: 243 SEFYVMVGDALQVLTNGRFVSVRHRVLTNTTK-PRMSMMYFAAPPLNWLISPLSKMVTAH 302

Query: 301 EQSLYKPFTWAQYKKAAYSLRLADSRLDLFKL 329
              LY+PFTWAQYK+AAY+LRL D+RLD FK+
Sbjct: 303 SPCLYRPFTWAQYKQAAYALRLGDTRLDQFKV 333

BLAST of HG10023042 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 174/334 (52.10%), Postives = 224/334 (67.07%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAV-----GIPTIDLS-QSRATTSKSIIDASQDFGFFKLINHGVSLDA 60
           +P  TP++T   K +       P ID S   R+  S+ I+ A +  GFFK+INHGV  + 
Sbjct: 3   LPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEI 62

Query: 61  IASLESHAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESS 120
           I   E    +FF K   +K RAGP +PFGYGCK+IG NGDLG+LEYLLL ++PT+V + S
Sbjct: 63  IKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKS 122

Query: 121 TSISNDPP-TFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRI 180
            +IS+D P  FS A N+Y++ VR+L CEI+DL  E LW    S  S LIRDV +DS+LR+
Sbjct: 123 ETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRL 182

Query: 181 NYYPGVKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVC 240
           N+YP         P+P +     +IGFGEHSDPQILT+LRSNDVDGL+I  +DG+W+P+ 
Sbjct: 183 NHYP---------PAPYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIP 242

Query: 241 PDPSAFYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMM 300
            DP+ F+VL+GD  +A+TNGRF SVRHR +AN +K PRMS MYF APPL A I+PL +M+
Sbjct: 243 SDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMV 302

Query: 301 LPNEQSLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
            P     Y  FTW  YKKA YSLRL   RL+ FK
Sbjct: 303 SPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of HG10023042 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.2e-80
Identity = 152/314 (48.41%), Postives = 212/314 (67.52%), Query Frame = 0

Query: 16  GIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQKTAPEKHR 75
           GIP +DL+   A     I++A +DFGFFKL+NHGV L+ +A+LE+ A  FF+K+  EK R
Sbjct: 26  GIPVVDLTHPDA--KNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85

Query: 76  AGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSI-SNDPPTFSYAVNEYVKN 135
           AGPP+PFGYG K IGPNGD+G +EYLLL ++P  +   S  I   +P  F   V  Y+  
Sbjct: 86  AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRAVVENYITA 145

Query: 136 VRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTENWEPSPKSHPS 195
           V+ +   +L+L+ EGL +  ++  S L++D  +DS  R+N+YP         P P+    
Sbjct: 146 VKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYP---------PCPEVQAL 205

Query: 196 N-NRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIGDAFKAMTNG 255
           N N +GFGEH+DPQI+++LRSN   GLQI L DG WV V PD ++F++ +GDA + MTNG
Sbjct: 206 NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQVMTNG 265

Query: 256 RFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQYKKAA 315
           RF SV+HR +A+ +K+ R+S++YFG P L+  IAPL  +ML  E+ LYK FTW +YKKAA
Sbjct: 266 RFKSVKHRVLADTTKS-RLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEYKKAA 325

Query: 316 YSLRLADSRLDLFK 328
           Y+ RLAD+RL  F+
Sbjct: 326 YTSRLADNRLAPFQ 327

BLAST of HG10023042 vs. ExPASy Swiss-Prot
Match: Q5ZA21 (Gibberellin 2-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX2 PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 6.6e-78
Identity = 150/332 (45.18%), Postives = 212/332 (63.86%), Query Frame = 0

Query: 10  KKTKAVGIPTIDLS--QSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQ 69
           ++ + V +PT+D+S    R   S+ +  A    GFF+ +NHGV     A++++ A  FF 
Sbjct: 27  RRGRGVVVPTVDMSAPAGRGELSRQVARACAGSGFFRAVNHGVPPRVSAAMDAAAAAFFV 86

Query: 70  KTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSIS-NDPPTFSY 129
           +   EK  AGPP+P GYG +SIG NGD+G+LEYL+L + P +V   +++I   DP  FS 
Sbjct: 87  RAGAEKQLAGPPDPLGYGSRSIGANGDVGELEYLILHASPDAVARKASAIDREDPRRFSQ 146

Query: 130 AVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYP------GVK 189
            VN+YV+ VR+L C +LDL+GEGL L + +  + LI    NDS++RIN+YP         
Sbjct: 147 VVNDYVEAVRQLACHVLDLLGEGLGLRDPTSLTRLITATDNDSLIRINHYPPSCAAAAGD 206

Query: 190 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQ------DGIWVPVCP 249
           H     P+P +      IGFGEH+DPQIL++LR+ND DGLQ+ L       D +WVPV P
Sbjct: 207 HKSGGGPAPTA-----AIGFGEHTDPQILSVLRANDADGLQLLLPDAAAAGDSVWVPVPP 266

Query: 250 DPSAFYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMML 309
           DPSAF+V +GD  +A+TNGR +S+RHR +    K PR+S +YF APPL+A I+ L E + 
Sbjct: 267 DPSAFFVNVGDLLQALTNGRLVSIRHRVVVGTGK-PRLSTIYFAAPPLHARISALPETVA 326

Query: 310 PNEQSLYKPFTWAQYKKAAYSLRLADSRLDLF 327
                 Y+ FTWA+YK+  Y+LRL+ +RLDLF
Sbjct: 327 AGAPRRYRAFTWAEYKRTMYTLRLSHNRLDLF 352

BLAST of HG10023042 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-76
Identity = 160/318 (50.31%), Postives = 199/318 (62.58%), Query Frame = 0

Query: 17  IPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQKTAPEKHRA 76
           IP ID+SQ R+  S  I+ A +  GFFK+INHGV    I+ +E  + +FF K A EK   
Sbjct: 15  IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74

Query: 77  GPPN-PFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISNDPP-----TFSYAVNE 136
            P N PF YG + IG NGD G++EYLL               +NDP      +FS AVN 
Sbjct: 75  RPVNQPFRYGFRDIGLNGDSGEVEYLLFH-------------TNDPAFRSQLSFSSAVNC 134

Query: 137 YVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTENWEPSPK 196
           Y++ V++L  EILDL  EGL +P  S FS LI  V +DSVLR+N+YP             
Sbjct: 135 YIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSD 194

Query: 197 SHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIGDAFKAM 256
              S  R+GFGEH+DPQILT+LRSN V GLQ+S  DG+WV V PDPSAF V +GD  + M
Sbjct: 195 QSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVM 254

Query: 257 TNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEM-MLPNEQSLYKPFTWAQY 316
           TNGRF+SVRHRA+    ++ R+S  YF  PPL A I PLS M M  N+  LY+ FTW +Y
Sbjct: 255 TNGRFISVRHRALTYGEES-RLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEY 314

Query: 317 KKAAYSLRLADSRLDLFK 328
           KK AYSLRL DSRLD+F+
Sbjct: 315 KKRAYSLRLEDSRLDMFR 317

BLAST of HG10023042 vs. ExPASy TrEMBL
Match: S0BDY8 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox2 PE=2 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.1e-163
Identity = 285/329 (86.63%), Postives = 306/329 (93.01%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT +RTKKT+AVGIPTIDLSQS+A TSKSI++A QDFGFFKLINHGVS  AIA LES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIVEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRS-DP-TSVVESSTSIS 120
             FDFFQK + EKHRAGP NPFGYGCKSIG NGD+GDLEYLLL S DP TS+++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180
           NDP TFS+ VNEYVK VR+LGCEILDL+GEGLWLPNKSVFSNLIRDVH+DSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V+HTEN E S KSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISLQDGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLQDGIWVPVCPDPSA 240

Query: 241 FYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQ 300
           FYVLIGD F+AMTNGRF+SVRHRAMANNSKNPRMSIMYFGAPPLN WIAPLSEMMLPN++
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
           SLYKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 329

BLAST of HG10023042 vs. ExPASy TrEMBL
Match: A0A0A0K6E5 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G413380 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.1e-163
Identity = 285/329 (86.63%), Postives = 306/329 (93.01%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT +RTKKT+AVGIPTIDLSQS+A TSKSII+A QDFGFFKLINHGVS  AIA LES
Sbjct: 1   MPCPTSIRTKKTRAVGIPTIDLSQSKAITSKSIIEACQDFGFFKLINHGVSHHAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRS-DP-TSVVESSTSIS 120
             FDFFQK + EKHRAGP NPFGYGCKSIG NGD+GDLEYLLL S DP TS+++SSTSIS
Sbjct: 61  QGFDFFQKPSSEKHRAGPANPFGYGCKSIGSNGDMGDLEYLLLPSHDPATSILQSSTSIS 120

Query: 121 NDPPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPG 180
           NDP TFS+ VNEYVK VR+LGCEILDL+GEGLWLPNKSVFSNLIRDVH+DSVLRINYYPG
Sbjct: 121 NDPLTFSHVVNEYVKGVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPG 180

Query: 181 VKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSA 240
           V+HTEN E S KSHPSNNRIGFGEHSDPQ+LTILR+NDVDGLQISL+DGIWVPVCPDPSA
Sbjct: 181 VQHTENRELSTKSHPSNNRIGFGEHSDPQVLTILRANDVDGLQISLRDGIWVPVCPDPSA 240

Query: 241 FYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQ 300
           FYVLIGD F+AMTNGRF+SVRHRAMANNSKNPRMSIMYFGAPPLN WIAPLSEMMLPN++
Sbjct: 241 FYVLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPPLNTWIAPLSEMMLPNDK 300

Query: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
           SLYKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 SLYKPFTWAQYKKAAYSLRLADSRLDLFK 329

BLAST of HG10023042 vs. ExPASy TrEMBL
Match: A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 283/327 (86.54%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT MRTKKTKAVGIPTIDLSQ+R TTSKSI+ A   FGFFKLINHGVS  AI+ LE 
Sbjct: 1   MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
           HAFDFF KTAPEKHRAGP  PFGYG K+IGPNGD GDLEYLLLRSDP SV+++S SISND
Sbjct: 61  HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFSY VNEYVK+V++LGCEILDLVGEGLWLPNKSVFS+LIRDVH+DSVLRINYYP VK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           HTENWE S KS+PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDG+W PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGDA +AMTNGRF+SVRHRAMANNSK  RMSIMYFGAPPLNAWIAPLSEM+LPN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEMILPNHQSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. ExPASy TrEMBL
Match: A0A6J1JXB5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488342 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.4e-160
Identity = 279/327 (85.32%), Postives = 298/327 (91.13%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT MRTKKT+AVGIPTIDLSQ+R TTSKSI+ A   FGFFKLINHGVS  AI+ LE 
Sbjct: 1   MPCPTAMRTKKTEAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLEC 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
            AFDFF KTAPEKHRAGP  PFGYG K+IGPNGD GDLEYLLLRSDP SV+++S SISND
Sbjct: 61  QAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFSY VNEYVK+V++LGCEILDLVGEGLWLPNKSVFS+LIRDVH+DSVLRINYYP VK
Sbjct: 121 PSTFSYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRINYYPAVK 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           HTENWE S KS+PSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDG+W PVCPDPSAFY
Sbjct: 181 HTENWELSSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGDA +AMTNGRF+SVRHRAMAN+SK  RMSIMYFGAPPLNAWIAPLSEM+ PN QSL
Sbjct: 241 VLIGDALQAMTNGRFVSVRHRAMANSSKKARMSIMYFGAPPLNAWIAPLSEMIPPNHQSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. ExPASy TrEMBL
Match: A0A1S3CFZ0 (gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103500006 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.1e-158
Identity = 276/327 (84.40%), Postives = 296/327 (90.52%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLES 60
           MPCPT +RTKKTKAVGIPTIDLSQSRA TSKSI++A Q+FGFFKLINHGV   AIA LES
Sbjct: 1   MPCPTSIRTKKTKAVGIPTIDLSQSRAITSKSIVEACQEFGFFKLINHGVPHHAIAQLES 60

Query: 61  HAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISND 120
             FDFFQK + EKHRAGPPNPFGYGCKSIG NGD+GDLEYLLL S   +     +SISND
Sbjct: 61  QGFDFFQKPSSEKHRAGPPNPFGYGCKSIGSNGDMGDLEYLLLPSHNPATSILQSSISND 120

Query: 121 PPTFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVK 180
           P TFS+ VNEYVK+VR+LGCEILDL+GEGLWLPNKSVFSNLIRDVH+DSVLRINYYPGV+
Sbjct: 121 PLTFSHVVNEYVKSVRDLGCEILDLIGEGLWLPNKSVFSNLIRDVHSDSVLRINYYPGVQ 180

Query: 181 HTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFY 240
           H EN E S KSHPSN+RIGFGEHSDPQILTILR+NDVDGLQISLQD IWVPVCPDPSAFY
Sbjct: 181 HIENRELSTKSHPSNDRIGFGEHSDPQILTILRANDVDGLQISLQDSIWVPVCPDPSAFY 240

Query: 241 VLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSL 300
           VLIGD F+AMTNGRF+SVRHRAMANNSKNPRMSIMYFGAP LN WIAPLSEMMLPN++SL
Sbjct: 241 VLIGDVFQAMTNGRFVSVRHRAMANNSKNPRMSIMYFGAPSLNTWIAPLSEMMLPNDKSL 300

Query: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 328
           YKPFTWAQYKKAAYSLRLADSRLDLFK
Sbjct: 301 YKPFTWAQYKKAAYSLRLADSRLDLFK 327

BLAST of HG10023042 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 344.0 bits (881), Expect = 1.4e-94
Identity = 174/334 (52.10%), Postives = 224/334 (67.07%), Query Frame = 0

Query: 1   MPCPTPMRTKKTKAV-----GIPTIDLS-QSRATTSKSIIDASQDFGFFKLINHGVSLDA 60
           +P  TP++T   K +       P ID S   R+  S+ I+ A +  GFFK+INHGV  + 
Sbjct: 3   LPSSTPLQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEI 62

Query: 61  IASLESHAFDFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESS 120
           I   E    +FF K   +K RAGP +PFGYGCK+IG NGDLG+LEYLLL ++PT+V + S
Sbjct: 63  IKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKS 122

Query: 121 TSISNDPP-TFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRI 180
            +IS+D P  FS A N+Y++ VR+L CEI+DL  E LW    S  S LIRDV +DS+LR+
Sbjct: 123 ETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRL 182

Query: 181 NYYPGVKHTENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVC 240
           N+YP         P+P +     +IGFGEHSDPQILT+LRSNDVDGL+I  +DG+W+P+ 
Sbjct: 183 NHYP---------PAPYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIP 242

Query: 241 PDPSAFYVLIGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMM 300
            DP+ F+VL+GD  +A+TNGRF SVRHR +AN +K PRMS MYF APPL A I+PL +M+
Sbjct: 243 SDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMV 302

Query: 301 LPNEQSLYKPFTWAQYKKAAYSLRLADSRLDLFK 328
            P     Y  FTW  YKKA YSLRL   RL+ FK
Sbjct: 303 SPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of HG10023042 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 160/318 (50.31%), Postives = 199/318 (62.58%), Query Frame = 0

Query: 17  IPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQKTAPEKHRA 76
           IP ID+SQ R+  S  I+ A +  GFFK+INHGV    I+ +E  + +FF K A EK   
Sbjct: 15  IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74

Query: 77  GPPN-PFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSISNDPP-----TFSYAVNE 136
            P N PF YG + IG NGD G++EYLL               +NDP      +FS AVN 
Sbjct: 75  RPVNQPFRYGFRDIGLNGDSGEVEYLLFH-------------TNDPAFRSQLSFSSAVNC 134

Query: 137 YVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTENWEPSPK 196
           Y++ V++L  EILDL  EGL +P  S FS LI  V +DSVLR+N+YP             
Sbjct: 135 YIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSD 194

Query: 197 SHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIGDAFKAM 256
              S  R+GFGEH+DPQILT+LRSN V GLQ+S  DG+WV V PDPSAF V +GD  + M
Sbjct: 195 QSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVM 254

Query: 257 TNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEM-MLPNEQSLYKPFTWAQY 316
           TNGRF+SVRHRA+    ++ R+S  YF  PPL A I PLS M M  N+  LY+ FTW +Y
Sbjct: 255 TNGRFISVRHRALTYGEES-RLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEY 314

Query: 317 KKAAYSLRLADSRLDLFK 328
           KK AYSLRL DSRLD+F+
Sbjct: 315 KKRAYSLRLEDSRLDMFR 317

BLAST of HG10023042 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 148/318 (46.54%), Postives = 204/318 (64.15%), Query Frame = 0

Query: 11  KTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDFFQKTA 70
           K + V IP IDL+ S A T   I+ A ++FGFFK+INHGV  D +  LE  A +FF    
Sbjct: 21  KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80

Query: 71  PEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTSVVESSTSI-SNDPPTFSYAVN 130
             K +AGPP+PFGYG K IGPNGDLG LEY+LL ++       +T+I  + P  F  AV 
Sbjct: 81  SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVE 140

Query: 131 EYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTENWEPSP 190
           EY+K ++ +  + L++V E L +  K   S L++   +DS LR+N+YP  + T       
Sbjct: 141 EYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEET------- 200

Query: 191 KSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIGDAFKA 250
              P    IGFGEH+DPQ++++LRSND +GLQI ++DG WV V PD S+F+VL+GD  + 
Sbjct: 201 ---PVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQV 260

Query: 251 MTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSLYKPFTWAQY 310
           MTNGRF SV+HR +  N+K  R+S++YF  PPL+  IAPLS ++   +  LY  FTW+QY
Sbjct: 261 MTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQY 320

Query: 311 KKAAYSLRLADSRLDLFK 328
           K +AY  +L D RL LF+
Sbjct: 321 KLSAYKTKLGDYRLGLFE 325

BLAST of HG10023042 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 143/325 (44.00%), Postives = 209/325 (64.31%), Query Frame = 0

Query: 4   PTPMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAF 63
           P P+ T  +    IP ++L+   A T   I+ A ++FGFFK++NHGV  + +  LE  A 
Sbjct: 22  PVPVLTSHS----IPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAI 81

Query: 64  DFFQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDP-TSVVESSTSISNDPP 123
            FF      K+RAGPP P+GYG K IGPNGD+G +EYLLL ++P  S  ++S      P 
Sbjct: 82  GFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQ 141

Query: 124 TFSYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHT 183
            F  +V EY+K ++E+  ++L++V E L +  +   S ++RD  +DS LR+N+YP  +  
Sbjct: 142 IFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPAAE-- 201

Query: 184 ENWEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVL 243
           E  E   K       +GFGEH+DPQI+++LRSN+  GLQI ++DG WV V PD S+F++ 
Sbjct: 202 EEAEKMVK-------VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFIN 261

Query: 244 IGDAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSLYK 303
           +GDA + MTNGRF SV+HR +A +++  R+S++YFG PPL+  IAPL  ++   +  LYK
Sbjct: 262 VGDALQVMTNGRFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYK 321

Query: 304 PFTWAQYKKAAYSLRLADSRLDLFK 328
            FTW+QYK +AY  +L D RL LF+
Sbjct: 322 EFTWSQYKSSAYKSKLGDYRLGLFE 330

BLAST of HG10023042 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 252.3 bits (643), Expect = 5.4e-67
Identity = 133/323 (41.18%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 6   PMRTKKTKAVGIPTIDLSQSRATTSKSIIDASQDFGFFKLINHGVSLDAIASLESHAFDF 65
           P+   K+    IP ID+S     +  +++ A +DFGFFK+INHGVS + ++ LE    DF
Sbjct: 7   PVAIPKSGFSLIPVIDMSDPE--SKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDF 66

Query: 66  FQKTAPEKHRAGPPNPFGYGCKSIGPNGDLGDLEYLLLRSDPTS-VVESSTSISNDPPTF 125
           F     EK +     PFGYG   IG NGD+G +EYLL+ ++  S       S+   P TF
Sbjct: 67  FSLPKSEKTQVA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTF 126

Query: 126 SYAVNEYVKNVRELGCEILDLVGEGLWLPNKSVFSNLIRDVHNDSVLRINYYPGVKHTEN 185
             A+ EY  +VR++  ++L+ + +GL +  ++  S L+ D + DS+LR+N+YP    +  
Sbjct: 127 RNALEEYTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLS-- 186

Query: 186 WEPSPKSHPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGIWVPVCPDPSAFYVLIG 245
              + K++   N IGFGEH+DPQI+++LRSN+  GLQI+L DG W+ V PD ++F+  +G
Sbjct: 187 ---NKKTNGGKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVG 246

Query: 246 DAFKAMTNGRFLSVRHRAMANNSKNPRMSIMYFGAPPLNAWIAPLSEMMLPNEQSLYKPF 305
           D+ + MTNGRF SVRHR +A N K  R+S++YF  P L   IAPL+ ++   ++ LY+ F
Sbjct: 247 DSLQVMTNGRFKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEF 306

Query: 306 TWAQYKKAAYSLRLADSRLDLFK 328
           TW++YK + Y+ RL+D+RL  F+
Sbjct: 307 TWSEYKNSTYNSRLSDNRLQQFE 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896324.11.6e-16688.99gibberellin 2-beta-dioxygenase 2 [Benincasa hispida][more]
NP_001292648.18.4e-16386.63gibberellin 2-beta-dioxygenase 2 [Cucumis sativus] >CCD28486.1 GA 2-oxidase [Cuc... [more]
KGN45038.11.9e-16286.63hypothetical protein Csa_016303 [Cucumis sativus][more]
XP_022954040.13.2e-16286.54gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata][more]
XP_023547502.19.3e-16286.24gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1... [more]
Match NameE-valueIdentityDescription
Q9XHM54.1e-11259.04Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1[more]
Q9FZ211.9e-9352.10Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ... [more]
Q9XG833.2e-8048.41Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Q5ZA216.6e-7845.18Gibberellin 2-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Q9C7Z12.8e-7650.31Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ... [more]
Match NameE-valueIdentityDescription
S0BDY84.1e-16386.63GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox2 PE=2 SV=1[more]
A0A0A0K6E59.1e-16386.63Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A6J1GRC31.5e-16286.54gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JXB59.4e-16085.32gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148834... [more]
A0A1S3CFZ05.1e-15884.40gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103500006 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G02400.11.4e-9452.10gibberellin 2-oxidase 6 [more]
AT1G47990.12.0e-7750.31gibberellin 2-oxidase 4 [more]
AT2G34555.11.7e-7646.54gibberellin 2-oxidase 3 [more]
AT1G30040.11.3e-7344.00gibberellin 2-oxidase [more]
AT1G78440.15.4e-6741.18Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 239..265
score: 25.59
coord: 203..224
score: 32.23
coord: 33..50
score: 51.52
NoneNo IPR availablePANTHERPTHR47990:SF45GIBBERELLIN 2-BETA-DIOXYGENASE 6coord: 2..328
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 2..328
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 6..319
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 168..278
e-value: 9.2E-22
score: 77.4
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 17..83
e-value: 6.2E-11
score: 43.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 3..318
e-value: 5.9E-70
score: 238.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 166..280
score: 11.570894

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10023042.1HG10023042.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding