HG10022857 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022857
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAdenylate isopentenyltransferase 3
LocationChr05: 29024327 .. 29025319 (+)
RNA-Seq ExpressionHG10022857
SyntenyHG10022857
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAAATGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

mRNA sequence

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAAATGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

Coding sequence (CDS)

ATGAATGTGTCCACCATGTTCATGAGCAAACAGATCACTCAACAACCATTGATAAATCTTCCCCCAGGAAGACTAAACACCAACCCTTTCTTCAACTTTAGGAAGCTGCAAAAAGAGAAGGTGGTAGTCGTAATCGGCGCCACCGGAACTGGAAAATCTCGGCTGTCGATCGACATCGCCACCCAATTCCCATCGGAAGTCATAAACTCCGACAAAATGCAAGTCTACGACGGCCTCGACATAGTAACAAACAAAATCACAAAGCAAGAACAACGTGGCGTTTCTCATCACTTACTCGGAGTTATCAATCCCTTCTCCGATTTCTCTGCAACTAACTTCTCCGATATGGCTATGCTTTCCTTGGATGCCATTTCTAGCCATGATCGTCTCCCAATCATCGCCGGTGGCTCAAATTCTTATATTGAAGCCTTAATTGACTCGAAGTTTCGATCGAAATATGAATGTTGTTTCCTTTGGGTAGATGTGTCAATGCAAGTATTGCAATCCTTTGTATCCGAACGAGTAGACAAGATGGTAGAAAATGGAATGGTCGACGAGGCCAAAAAATTCTTCGATCCCGCCCGAGATTATTCTCGAGGGATCAAAAGGGCAATCGGAGTTCCGGAATTCGACTCGTATTTTCGATACAAATCATTGTTAGATGAGGAAGATCAAGAGATACTTTTGAAGGAATCCATATCAAAAGTGAAGGAGCATACATGTAAACTAGCATTGAAACAAATGGGAAAGATCCAACGGCTGAGAAATGTCAAAGGTTGGAACATACATAGATTGGATGCTACAGAGGCCTTTGCAAAGCAAGGGAAAGAAGCAGAGGAAGCATGGAACAAGCTCGTCGCCGTACCGAGCTCGACGATAATCGACCAGTTTCTTCACCCGAATGTCGTTGCCACAAAATTCCCGACCAATTTAGCATCCCTCATCAGCGTGCCGCGGAAGAGAAGTGCAGCCATGGCAGCTGCAACTCCTTGA

Protein sequence

MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHPNVVATKFPTNLASLISVPRKRSAAMAAATP
Homology
BLAST of HG10022857 vs. NCBI nr
Match: XP_038899604.1 (adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 605.1 bits (1559), Expect = 3.5e-169
Identity = 304/330 (92.12%), Postives = 317/330 (96.06%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M+VSTMFMSKQITQQPLINLPPGRLNTNPFFN RK QKEKVV+VIGATGTGKSRLS+DIA
Sbjct: 1   MDVSTMFMSKQITQQPLINLPPGRLNTNPFFNLRKPQKEKVVIVIGATGTGKSRLSVDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQRGV HHLLG+INPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYRGLDIVTNKITKQEQRGVPHHLLGIINPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAI+SHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAITSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDP RDYSRGIKRAIGVPEFDSYFRYK LLDEE Q+ LLKESISKVKEHT
Sbjct: 181 NGMVDEAEKFFDPTRDYSRGIKRAIGVPEFDSYFRYKPLLDEEAQDKLLKESISKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+Q+GKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV VPS+T+IDQFLHP
Sbjct: 241 CKLALRQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTVPSTTLIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NVVATKFPTNLASLI V RKRSAAMAAATP
Sbjct: 301 NVVATKFPTNLASLIGVSRKRSAAMAAATP 330

BLAST of HG10022857 vs. NCBI nr
Match: KAA0057613.1 (adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa] >TYK20984.1 adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 587.8 bits (1514), Expect = 5.8e-164
Identity = 298/330 (90.30%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN FFN RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQ+GKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of HG10022857 vs. NCBI nr
Match: XP_008451382.1 (PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo])

HSP 1 Score: 586.3 bits (1510), Expect = 1.7e-163
Identity = 298/330 (90.30%), Postives = 310/330 (93.94%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN F N RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQ+GKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of HG10022857 vs. NCBI nr
Match: XP_004136062.1 (adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus] >KGN44872.1 hypothetical protein Csa_015949 [Cucumis sativus])

HSP 1 Score: 579.3 bits (1492), Expect = 2.1e-161
Identity = 292/330 (88.48%), Postives = 309/330 (93.64%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMSK  TQQPLINLPPGRL TNPF   RKLQKEKVV+VIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR +YECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFRY+SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+Q+GKIQRLRNVKGWNIHRLDATEAF KQGKEA+EAWNK V +PS+TIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ATKFPTNLASLI VPRK+SAAMAAATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

BLAST of HG10022857 vs. NCBI nr
Match: XP_023004596.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 280/330 (84.85%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LD I   DRLPIIAGGSNSYIEALIDS F+SKYECCFLWVDVSM +L SFVS+RVDKMV 
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMV+EAK+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +QMGKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ T  PTNLASL+ +PRKRSAA+AAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of HG10022857 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.6e-86
Identity = 162/304 (53.29%), Postives = 220/304 (72.37%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVVV++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+F +E+INSDK+QV+ GLDIVTNKIT +E  GV HHLLGV+ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISSHDRLPIIAGGSNSYIEALID---SKFRSKYECCFLWVDVSMQVLQSFVSERVDK 180
           ++++ +  +LPII GGSNSY+EAL+D   +KFRS+Y+CCFLWVDV++ VL  FVSERVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVENGMVDEAKKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKV 240
           MVE+GMV+E ++FFD +  DYSRGIK+AIG PEFD +FR +  L+ ED+E LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGK--EAEEAWNKLVAVPSSTII 299
           K +T +LA +Q  KI+RLR VK W+I R+DAT  F K+    +A  AW +LVA PS+  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of HG10022857 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 5.8e-82
Identity = 160/289 (55.36%), Postives = 212/289 (73.36%), Query Frame = 0

Query: 15  QPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+AT+FP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPII 134
           VY GLDIVTNK+T +E  GV HHLLG + + + DF+A +F   A+ ++++I   DR+PII
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIEALIDS--KFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFF 194
           AGGSNSYIEAL++    FR +Y CCFLWVDVS  VL SFVSERVDKMV+ G+VDE ++ F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-ARDYSRGIKRAIGVPEFDSYFRYKSL-LDEEDQEILLKESISKVKEHTCKLALKQMG 254
           DP + DYS GI+RAIGVPE D + R +      E  E LL+ +I K+KE+TC LA +Q+ 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  VA PS+  +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of HG10022857 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 5.4e-72
Identity = 138/283 (48.76%), Postives = 204/283 (72.08%), Query Frame = 0

Query: 24  RLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+AT+F  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIE 143
           NK+T +E RGV HHLLGV  +   + +AT +S +A  ++  +S++++LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSK---FRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSR 203
           AL++       + Y+CCF+WVDVS+ VL SFVS+RVD+M+E G+++E ++ F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQMGKIQRLRNVKG 263
           GI+RAIGVPE   Y R +SL+D   +  +L  ++  +K++T  LA +Q+ KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFAKQG-KEAEEAWNKLVAVPSSTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LVA PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of HG10022857 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.5e-61
Identity = 134/317 (42.27%), Postives = 193/317 (60.88%), Query Frame = 0

Query: 21  PPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLD 80
           P     TN     ++ +KEK++V++GATGTGKSRLSID+A  FP EVINSDKMQVY GLD
Sbjct: 12  PTTTTTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLD 71

Query: 81  IVTNKITKQEQRGVSHHLLGVINPF-SDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNS 140
           I TNKI+  ++ GV HHLLG ++P   + +  +F  +A  ++  I+   +LP++ GGSNS
Sbjct: 72  ITTNKISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNS 131

Query: 141 YIEALIDSKFRS------------------KYECCFLWVDVSMQVLQSFVSERVDKMVEN 200
           +I AL+  +F S                  +Y+CCFLWVDVS++VL  ++++RVD M+E 
Sbjct: 132 FIHALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLEL 191

Query: 201 GMVDEAKKFFDPA-RDYSR------GIKRAIGVPEFDSYFRYKSLLDEEDQEI------- 260
           GM DE  +F+ P   D+        G+++AIGVPEFD YF      D E ++        
Sbjct: 192 GMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRR 251

Query: 261 -LLKESISKVKEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAF-----AKQGKEAEEA 299
              +E++  +KE+TC LA +Q+GKI RL+   GW++ RLDATE+F     +  G++  E 
Sbjct: 252 GAFEEAVRAIKENTCHLAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEI 311

BLAST of HG10022857 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-60
Identity = 134/336 (39.88%), Postives = 200/336 (59.52%), Query Frame = 0

Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQ-------KEKVVVVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVVV++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSD 123
           +D+AT+FPSE+INSDK+QVY+GL+I TN+IT Q++RGV HHLLGVINP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK----------------YECCFLW 183
            A   +  I+S  ++PIIAGGSNS++ AL+  +F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPAR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+++GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEEDQEILLKESISKVKEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAF 302
                K +  +  ++    +++  +K +T  LA +Q+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

BLAST of HG10022857 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 2.8e-164
Identity = 298/330 (90.30%), Postives = 311/330 (94.24%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN FFN RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQ+GKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of HG10022857 vs. ExPASy TrEMBL
Match: A0A1S3BRC2 (adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492689 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.2e-164
Identity = 298/330 (90.30%), Postives = 310/330 (93.94%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPPGRLNTN F N RKLQKEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR KYECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFR +SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLALKQ+GKIQRLRN+KGWNIHRLDATEAF KQGKEAEEAWNKLV +PS+TIIDQFLHP
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           N VATKFPTNLASLI VPRKRSAAMAAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

BLAST of HG10022857 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 292/330 (88.48%), Postives = 309/330 (93.64%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVS+MFMSK  TQQPLINLPPGRL TNPF   RKLQKEKVV+VIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDIVTNKITKQEQ GV HHLLG+INPF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LDAISSHDRLPIIAGGSNSYIEALIDS FR +YECCFLWVDVSMQVLQSFVSERVDKMV 
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA+KFFDPARDYSRGIKRAIGVPEFDSYFRY+SLLDEE QE LL+ESI+KVKEHT
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLAL+Q+GKIQRLRNVKGWNIHRLDATEAF KQGKEA+EAWNK V +PS+TIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ATKFPTNLASLI VPRK+SAAMAAATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

BLAST of HG10022857 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.7e-157
Identity = 280/330 (84.85%), Postives = 306/330 (92.73%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           TQFPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           LD I   DRLPIIAGGSNSYIEALIDS F+SKYECCFLWVDVSM +L SFVS+RVDKMV 
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMV+EAK+FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +QMGKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TII+QFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ T  PTNLASL+ +PRKRSAA+AAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

BLAST of HG10022857 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 277/330 (83.94%), Postives = 304/330 (92.12%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           MNVSTMFMS+QITQQPLINLPP R+NTNPFFN RKL+KEKVV+VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVYDGLDIVTNK+TKQEQRGV HHLLG+I+PFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDAISSHDRLPIIAGGSNSYIEALIDSKFRSKYECCFLWVDVSMQVLQSFVSERVDKMVE 180
           L  I   DRLPIIAGGSNSY+EALIDS F+SKYECCFLWVDVSM VL SFVS+RVDKMV 
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAKKFFDPARDYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHT 240
           NGMVDEA++FFDPA DYSRGIKRAIGVPEFDSYFRY+SLLD+E QE LL+E+ISKVK++T
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFLHP 300
           CKLA +QMGKIQRLRN+KGW IHRLDATEAF KQGKEAEE WNKLV VPS+TIIDQFLH 
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVVATKFPTNLASLISVPRKRSAAMAAATP 331
           NV+ TK PTNLA+L+ + R RSAA+AAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

BLAST of HG10022857 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 318.9 bits (816), Expect = 4.7e-87
Identity = 162/304 (53.29%), Postives = 220/304 (72.37%), Query Frame = 0

Query: 1   MNVSTMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIA 60
           M +S     + +   P ++ PP R   N         K+KVVV++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINPFSDFSATNFSDMAMLS 120
           T+F +E+INSDK+QV+ GLDIVTNKIT +E  GV HHLLGV+ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDAISSHDRLPIIAGGSNSYIEALID---SKFRSKYECCFLWVDVSMQVLQSFVSERVDK 180
           ++++ +  +LPII GGSNSY+EAL+D   +KFRS+Y+CCFLWVDV++ VL  FVSERVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVENGMVDEAKKFFDPAR-DYSRGIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKV 240
           MVE+GMV+E ++FFD +  DYSRGIK+AIG PEFD +FR +  L+ ED+E LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAFAKQGK--EAEEAWNKLVAVPSSTII 299
           K +T +LA +Q  KI+RLR VK W+I R+DAT  F K+    +A  AW +LVA PS+  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

BLAST of HG10022857 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 305.8 bits (782), Expect = 4.1e-83
Identity = 160/289 (55.36%), Postives = 212/289 (73.36%), Query Frame = 0

Query: 15  QPLINLPPGRLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQ 74
           QPL     G +   PFF     +K+KVV V+GATGTGKSRL+ID+AT+FP+E++NSDK+Q
Sbjct: 13  QPLSLNFQGNMVDVPFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQ 72

Query: 75  VYDGLDIVTNKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPII 134
           VY GLDIVTNK+T +E  GV HHLLG + + + DF+A +F   A+ ++++I   DR+PII
Sbjct: 73  VYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPII 132

Query: 135 AGGSNSYIEALIDS--KFRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFF 194
           AGGSNSYIEAL++    FR +Y CCFLWVDVS  VL SFVSERVDKMV+ G+VDE ++ F
Sbjct: 133 AGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIF 192

Query: 195 DP-ARDYSRGIKRAIGVPEFDSYFRYKSL-LDEEDQEILLKESISKVKEHTCKLALKQMG 254
           DP + DYS GI+RAIGVPE D + R +      E  E LL+ +I K+KE+TC LA +Q+ 
Sbjct: 193 DPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQ 252

Query: 255 KIQRLRNVKGWNIHRLDATEAFAKQGKEAEEAWNKLVAVPSSTIIDQFL 299
           KIQRL     WN+HR+DATE F ++G+EA+EAW+  VA PS+  +++FL
Sbjct: 253 KIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

BLAST of HG10022857 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 272.7 bits (696), Expect = 3.9e-73
Identity = 138/283 (48.76%), Postives = 204/283 (72.08%), Query Frame = 0

Query: 24  RLNTNPFFNFRKLQKEKVVVVIGATGTGKSRLSIDIATQFPSEVINSDKMQVYDGLDIVT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+AT+F  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKITKQEQRGVSHHLLGVI-NPFSDFSATNFSDMAMLSLDAISSHDRLPIIAGGSNSYIE 143
           NK+T +E RGV HHLLGV  +   + +AT +S +A  ++  +S++++LPI+AGGSNSYIE
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSK---FRSKYECCFLWVDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYSR 203
           AL++       + Y+CCF+WVDVS+ VL SFVS+RVD+M+E G+++E ++ F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYKSLLDEEDQEILLKESISKVKEHTCKLALKQMGKIQRLRNVKG 263
           GI+RAIGVPE   Y R +SL+D   +  +L  ++  +K++T  LA +Q+ KIQRL     
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWK 262

Query: 264 WNIHRLDATEAFAKQG-KEAEEAWNKLVAVPSSTIIDQFLHPN 302
            ++HR+DATE F K+  +E +EAW  LVA PS  I+D+F + N
Sbjct: 263 MSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

BLAST of HG10022857 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 233.4 bits (594), Expect = 2.6e-61
Identity = 134/336 (39.88%), Postives = 200/336 (59.52%), Query Frame = 0

Query: 4   STMFMSKQITQQPLINLPPGRLNTNPFFNFRKLQ-------KEKVVVVIGATGTGKSRLS 63
           S  F S   +   L++    R    P  +  +++       K+KVVV++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSD 123
           +D+AT+FPSE+INSDK+QVY+GL+I TN+IT Q++RGV HHLLGVINP   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK----------------YECCFLW 183
            A   +  I+S  ++PIIAGGSNS++ AL+  +F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMQVLQSFVSERVDKMVENGMVDEAKKFFDPAR---DYSRGIKRAIGVPEFDSYFR- 243
           VDVS  VL  ++  RVD+M+++GM +E  +F+DP +   +   GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YKSLLDEEDQEILLKESISKVKEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEAF 302
                K +  +  ++    +++  +K +T  LA +Q+ KI+ L++  GW I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDA-GWEIERVDATASF 322

BLAST of HG10022857 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 226.5 bits (576), Expect = 3.2e-59
Identity = 135/328 (41.16%), Postives = 200/328 (60.98%), Query Frame = 0

Query: 2   NVSTMFMSKQI--TQQPLINLPPGRLNTNPFFN--FRKLQKEKVVVVIGATGTGKSRLSI 61
           N+++ F+S  +     P + LPP R +  P       +  K+KVVV++GATG+GKS LSI
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPR-SVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSI 62

Query: 62  DIATQFPSEVINSDKMQVYDGLDIVTNKITKQEQRGVSHHLLGVINP-FSDFSATNFSDM 121
           D+AT+F  E++NSDK+Q YDGL + TN+++  E+ GV HHLLG + P  S+ + + F  +
Sbjct: 63  DLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSL 122

Query: 122 AMLSLDAISSHDRLPIIAGGSNSYIEALIDSKFRSK---------------YECCFLWVD 181
           A  S+  I++   LPIIAGGSNS+I AL+  +F  K               YECCFLWVD
Sbjct: 123 ASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVD 182

Query: 182 VSMQVLQSFVSERVDKMVENGMVDEAKKFFDPARDYS----RGIKRAIGVPEFDSYF--- 241
           VS+ VL  ++S+RVD+M+E+GM +E   F+DP    S     GI + IG+PEFD YF   
Sbjct: 183 VSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLY 242

Query: 242 --RYKSLLDEEDQ--EILLKESISKVKEHTCKLALKQMGKIQRLRNVKGWNIHRLDATEA 299
               K  + E DQ  +    E++ ++KE+T +LA KQ+ +I +L++  GW+I RLDAT +
Sbjct: 243 PPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKS-SGWDIQRLDATPS 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038899604.13.5e-16992.12adenylate isopentenyltransferase 3, chloroplastic [Benincasa hispida][more]
KAA0057613.15.8e-16490.30adenylate isopentenyltransferase 3 [Cucumis melo var. makuwa] >TYK20984.1 adenyl... [more]
XP_008451382.11.7e-16390.30PREDICTED: adenylate isopentenyltransferase 3, chloroplastic [Cucumis melo][more]
XP_004136062.12.1e-16188.48adenylate isopentenyltransferase 3, chloroplastic [Cucumis sativus] >KGN44872.1 ... [more]
XP_023004596.11.2e-15684.85adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q93WC96.6e-8653.29Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID25.8e-8255.36Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID15.4e-7248.76Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q5GHF72.5e-6142.27Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID33.7e-6039.88Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A5D3DBM72.8e-16490.30Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BRC28.2e-16490.30adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0K9191.0e-16188.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1[more]
A0A6J1KSJ25.7e-15784.85adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36... [more]
A0A6J1H8C91.2e-15483.94adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT3G63110.14.7e-8753.29isopentenyltransferase 3 [more]
AT5G19040.14.1e-8355.36isopentenyltransferase 5 [more]
AT3G23630.13.9e-7348.76isopentenyltransferase 7 [more]
AT1G68460.12.6e-6139.88isopentenyltransferase 1 [more]
AT3G19160.13.2e-5941.16ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01715IPPTcoord: 151..249
e-value: 8.6E-12
score: 45.3
coord: 74..147
e-value: 4.5E-20
score: 72.4
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 164..262
e-value: 1.0E-106
score: 358.0
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 33..301
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 38..298
e-value: 1.0E-106
score: 358.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 37..191
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 33..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022857.1HG10022857.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
cellular_component GO:0009536 plastid
molecular_function GO:0009824 AMP dimethylallyltransferase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity