HG10022827 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022827
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit
Location	Chr05: 28718270 .. 28721511 (+)
RNA-Seq Expression	HG10022827
Synteny	HG10022827

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGATGCCGGTAGAAGAAGGCGATACCAACAAAGATGTTGAGGTTGCTCCGGCCTTAATTGCAGTTCACCCGACCGAGAAATCTGTTGCCGTCGCCGTTGGCTCCGACCTGCGTGTATTCGATCTCGTGTGAGTTTTTTTTCGACCATTAATCTTATCTTCTATAAACTTGGATTGGTGGTACAATACGATTATGTTCACACTCTTTGTACTAATTTGATTTAAAATTAAATCGAACTGTACTTGCCTTTGTAGCCCTGTTGAATTGCTTTCTCGTGTGTTGATGGGTTGTTTTGAGTTAACTGCAGAGGTAATTGTCCGATTTCTTTGGTTGATGAATCTGGGGGACCGTATCATAAAGACTCCATTAGAACGATTTGTTATGGTTCGAAAGGCAAGCTTTTTGTATCTGCCGGTGATGATAAGACAGTTAAGATTTGGTCTACTGAGTCTTGGCGCTGTATTTATAATGTGTAAGTTTTTTCCCCCTTCTCTGCATTTTTTTTTCCTGGGTGATTTATTGCGGGGACGTTCATTGGCTGTGGATATGCGAGCTGATTGATGTGTTCATTGGCTGTGGCTTATCTATTGCGCAAATTTCTTTATTTTTTTTGTTATTACAATGCATATAGTTAGTAGCGTTTGGATTATCTGGAAAAATTGTAACACCAGCTGCATGCTTCCTCGATTGGTCTTCCTTGTGTAATACTGAAAATTTCATAATTCTGCTGACAGGGTTTCTGAAAAACGAGTGAGTGCAGCTGCCATTAGCCCTAATGGTCTGCACGTTTGTTTTGCTGACAAGTTTGGGGTTATCTGGGTTGTGGATCTTGATGGAGGGGTTGATGAAAATCAGCCTCTGGCCAACAAGAAGGCAGTTTCCTTGCTTTCACATTATTGCAGTATAATCACGAGCTTGGTAAGCAGTCCTCTCTTAACCATCTTTTTTTTTCTTCTTTTTTGCATCTGTGGCTTCTTTTTTTTCTTCTTTTTTGTAATGGGTGTAGGGGATCCGGAGGTTTGCATATTTATTTAGTTTGTTTGTTGTGATTGCCTGTGTATTTTGTTTTGGAATATATATTTTGTATTTACATTCCTCTTCATGAAATTTTCTTTTTCTAATCAAAAGCTAAAAAATCAAAATTTGGAGGAGGTCGATTCATATGATGCTATCATATATTTATAATGGTGGGAAATGTTTCTGCTGTTTGTATGGTTTTCATGCTTCCACATAATTAATATCTTTACTGTTGCAGGAATTCTCACTGGATGGGCGGTTCTTAGTTAGTGCGGACCGTGATTTTAAAATTCGGGTAATCATACTCTCTCCTCATTAATTATGAGAGTAGCTCATTGTAATTCACGTTTGTGAAAATGGGGTTGCTTTTGTTGAAGGTTACAACATTTCCCACAAAACCTGTGAATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACGGAGTGAGTTTCCATGATCTAAATCTAATATATTACAAGATAAAGGTGGATGGTTAGTAGTGGTGACATATTTTTAAGCGACATTTCCTTTGATTTTGGTTCATGTGTTTATTCCATGTGCTTTTTCTAGTTTCGTATCTTGCATAGCCTTCATTTCCAACCTGGAGTGCTCACATGGGTTTCTTAGCTCTGGAAGCGGTGATTCAACTGTAAGATTGTCTCATATGAACTTCTAATTTGCTTTTGGTGCAATATGTGAAATACAATAATATTTTGTGGCCTTTTCAGGTACGCTTATGGGATATTCTCTCTGGATCACTCCTTGACACTTGCGAGATTGGAGATAAGGTGTTTCTGTTGTCCTGAGCTTATTTTGAACCTCTAAACTCTCACTTCAATAGTTTGTTAAAAATTGAATTGATGAATTCTACATCGGATAGTACTAGGTTATCTCTCAGAATTATTACCTTGATTAATGAACTCTAAAGCCAGGACAAGATACTTGTTAACTTAGACTAGAGTTTTATTGTATCCCATATTAAGATTGGTGTACAATATTTTTGGATTGTTCCAGTTTATCTCTTCTGTTACTAGAAGAGGTAACTAAATTGTGCATCCTTCTCTTTTGTGGTATGGTTTTTGACCTCCTTGTTCACAACTTGATTTCTCACACTAACTTACTGAAGGTAGAACTCCTGGAGTCTAAAGAAGGAGAGGGCTGCCATTCAGCTGTCACAAGTCTCTGCACGATTGCTGATGGTGCCCTTGTTGCAGTGGCGGTTCAGAGGCAATTTCCCCCCTCTATGCTCTTTTCTCTTCTTTGGTGGAGTCTACCATTGAATTTATGTCCTAATTCATCCTATCATTTTTCTTTGTAGCTTGCATGGAATAGTTTTGCTGAACTGCGACCTGTCAAATCATACTCTATCTGTAGTCAAGGTAAATGACTCATGGCCAGTGGAGCAATCTCAATGGTTACAGTGACTGGGGGTTCTCCAGATTAATATTTTACAAACTTCTGGATGGCATATACCGCCAAGATATTTTGCCCTTTCCCCTCCCTAGAGATTTTTAGGATGGAAGTAATATTTGTATTGGGGGTGTTTTGGCCTGAGGAGTTGGAGTTGGGGGAAGTGGAGTTGTGAACTTTTGGTCCAAGAGTTTGTGGGCCCCACGACTAAAAAAGTATTAATTTATGCTTTATTAACTTTTTACATTGTAGGTCACACAACTTCACAAATCTCCAAACTTTATGAACTCCACACCTTCTCCTAAATACCCTCTTGATGTTTGTTAGCCTTTGAGTCTATTTACGGGCACATCGTCTAATTTATTTAATCTCGTTTCCAGATGAAATTAATTTGTTATAATTTTGATATTTCAGGTGGTTTCTTTTAGTGAGGAGACATTCATCCCAACCAGCCTATCGACCAGTTGCTCGACTGGGTATCTGTGGATGGTAACCGGTGTCTCCACTTTGCACAATTTGGGGCACTCCGCTCTGGCCCGTGTCAAGGTCATCAGTGGTTTCAACAAGGACAATCCCGATTCCGCCGTGAACGAGGTATCCATACTGGAAGATGATGCAATACCCGGGGGAATGAAGCTACTTGAAAAATTGCAGGGAGGTGTAACGTTCGACGAAAAGGTGTTCTTGGCAGCTGCTGAAGCTGTTAAAACTGCAATGAGCAATCTGTTGACTAAGAAACAGTACTCCACAGAGAAGCGGGACTTCAGAAAGAGAACAAGAAATGATAGGAAGCTTAAACAATGA

mRNA sequence

ATGGAAGAGATGCCGGTAGAAGAAGGCGATACCAACAAAGATGTTGAGGTTGCTCCGGCCTTAATTGCAGTTCACCCGACCGAGAAATCTGTTGCCGTCGCCGTTGGCTCCGACCTGCGTGTATTCGATCTCGTAGGTAATTGTCCGATTTCTTTGGTTGATGAATCTGGGGGACCGTATCATAAAGACTCCATTAGAACGATTTGTTATGGTTCGAAAGGCAAGCTTTTTGTATCTGCCGGTGATGATAAGACAGTTAAGATTTGGTCTACTGAGTCTTGGCGCTGTATTTATAATGTGGTTTCTGAAAAACGAGTGAGTGCAGCTGCCATTAGCCCTAATGGTCTGCACGTTTGTTTTGCTGACAAGTTTGGGGTTATCTGGGTTGTGGATCTTGATGGAGGGGTTGATGAAAATCAGCCTCTGGCCAACAAGAAGGCAGTTTCCTTGCTTTCACATTATTGCAGTATAATCACGAGCTTGGAATTCTCACTGGATGGGCGGTTCTTAGTTAGTGCGGACCGTGATTTTAAAATTCGGGTTACAACATTTCCCACAAAACCTGTGAATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACGGATTTCGTATCTTGCATAGCCTTCATTTCCAACCTGGAGTGCTCACATGGGTTTCTTAGCTCTGGAAGCGGTGATTCAACTGTACGCTTATGGGATATTCTCTCTGGATCACTCCTTGACACTTGCGAGATTGGAGATAAGGTGGTTTCTTTTAGTGAGGAGACATTCATCCCAACCAGCCTATCGACCAGTTGCTCGACTGGGTATCTGTGGATGGTAACCGGTGTCTCCACTTTGCACAATTTGGGGCACTCCGCTCTGGCCCGTGTCAAGGTCATCAGTGGTTTCAACAAGGACAATCCCGATTCCGCCGTGAACGAGGTATCCATACTGGAAGATGATGCAATACCCGGGGGAATGAAGCTACTTGAAAAATTGCAGGGAGGTGTAACGTTCGACGAAAAGGTGTTCTTGGCAGCTGCTGAAGCTGTTAAAACTGCAATGAGCAATCTGTTGACTAAGAAACAGTACTCCACAGAGAAGCGGGACTTCAGAAAGAGAACAAGAAATGATAGGAAGCTTAAACAATGA

Coding sequence (CDS)

ATGGAAGAGATGCCGGTAGAAGAAGGCGATACCAACAAAGATGTTGAGGTTGCTCCGGCCTTAATTGCAGTTCACCCGACCGAGAAATCTGTTGCCGTCGCCGTTGGCTCCGACCTGCGTGTATTCGATCTCGTAGGTAATTGTCCGATTTCTTTGGTTGATGAATCTGGGGGACCGTATCATAAAGACTCCATTAGAACGATTTGTTATGGTTCGAAAGGCAAGCTTTTTGTATCTGCCGGTGATGATAAGACAGTTAAGATTTGGTCTACTGAGTCTTGGCGCTGTATTTATAATGTGGTTTCTGAAAAACGAGTGAGTGCAGCTGCCATTAGCCCTAATGGTCTGCACGTTTGTTTTGCTGACAAGTTTGGGGTTATCTGGGTTGTGGATCTTGATGGAGGGGTTGATGAAAATCAGCCTCTGGCCAACAAGAAGGCAGTTTCCTTGCTTTCACATTATTGCAGTATAATCACGAGCTTGGAATTCTCACTGGATGGGCGGTTCTTAGTTAGTGCGGACCGTGATTTTAAAATTCGGGTTACAACATTTCCCACAAAACCTGTGAATGAAGCTCATGAGATTCAAAGTTTTTGTCTAGGCCATACGGATTTCGTATCTTGCATAGCCTTCATTTCCAACCTGGAGTGCTCACATGGGTTTCTTAGCTCTGGAAGCGGTGATTCAACTGTACGCTTATGGGATATTCTCTCTGGATCACTCCTTGACACTTGCGAGATTGGAGATAAGGTGGTTTCTTTTAGTGAGGAGACATTCATCCCAACCAGCCTATCGACCAGTTGCTCGACTGGGTATCTGTGGATGGTAACCGGTGTCTCCACTTTGCACAATTTGGGGCACTCCGCTCTGGCCCGTGTCAAGGTCATCAGTGGTTTCAACAAGGACAATCCCGATTCCGCCGTGAACGAGGTATCCATACTGGAAGATGATGCAATACCCGGGGGAATGAAGCTACTTGAAAAATTGCAGGGAGGTGTAACGTTCGACGAAAAGGTGTTCTTGGCAGCTGCTGAAGCTGTTAAAACTGCAATGAGCAATCTGTTGACTAAGAAACAGTACTCCACAGAGAAGCGGGACTTCAGAAAGAGAACAAGAAATGATAGGAAGCTTAAACAATGA

Protein sequence

MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPYHKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCFADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGSLLDTCEIGDKVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDSAVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRDFRKRTRNDRKLKQ

Homology

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BLAST of HG10022827 vs. NCBI nr
Match: XP_023549061.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pepo subsp. pepo] >XP_023549063.1 tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679.1 bits (1751), Expect = 2.2e-191
Identity = 352/433 (81.29%), Postives = 362/433 (83.60%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVE+APALIAVHP EKSVAVAVGSDLRVFDLVGNCP+SLVDESGG Y
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGGSY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGS G+LFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISPNG+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS LEC HGF+ SGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFIISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCEIGD                                                   
Sbjct: 241 LLATCEIGDKVELLDSKEREGCHSAVTSICTIADGGLVAVAIQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLS+SCST YLWMVTGVS LHNLGHS+LARVKVISGFNKDNP+S
Sbjct: 301 SVVKVVSISEETFIPTSLSSSCSTRYLWMVTGVSGLHNLGHSSLARVKVISGFNKDNPNS 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             NE+SILEDDAIPGG KLLEKLQGGVTFDEKVFLAA+EAVKTAMSNLLTKKQYSTEKRD
Sbjct: 361 TENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAASEAVKTAMSNLLTKKQYSTEKRD 420

[Close]

BLAST of HG10022827 vs. NCBI nr
Match: XP_038899582.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Benincasa hispida])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 354/433 (81.76%), Postives = 361/433 (83.37%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE  VEEGDTNKDVEVAPALIAVHPTEK VAVAVGSDLRV+DLVGN  +SLVDESG PY
Sbjct: 1   MEETTVEEGDTNKDVEVAPALIAVHPTEKFVAVAVGSDLRVYDLVGNSAVSLVDESGEPY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGSKG LFVSAGDDKTVKIW+TESWRCIYNVVSEKRVSAAAISPNGLHVCF
Sbjct: 61  HKDSIRTICYGSKGNLFVSAGDDKTVKIWATESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDG+FLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGKFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS+LECSHGFL SGSGD TVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISSLECSHGFLISGSGDLTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCEIGD                                                   
Sbjct: 241 LLYTCEIGDKVELLESKEREGCHSAVTSLCTIANGALVAVAVQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLST  STGYLWMVTG S+LHNLGHS+LARVKVISGFNKDNPDS
Sbjct: 301 SVVKVVSISEETFIPTSLSTCWSTGYLWMVTGASSLHNLGHSSLARVKVISGFNKDNPDS 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             NEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD
Sbjct: 361 TTNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 420

[Close]

BLAST of HG10022827 vs. NCBI nr
Match: XP_004146976.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucumis sativus] >KGN44796.1 hypothetical protein Csa_016847 [Cucumis sativus])

HSP 1 Score: 672.9 bits (1735), Expect = 1.6e-189
Identity = 347/433 (80.14%), Postives = 363/433 (83.83%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PV+EGDTNKDVEVAPALIA+HP+E SVAVAVGSDLRVFDLVGNC +SLVDESG PY
Sbjct: 1   MEETPVDEGDTNKDVEVAPALIALHPSENSVAVAVGSDLRVFDLVGNCAVSLVDESGPPY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGSKG+LF+SAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFISAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VT FPTKPVNEAHEIQSFCLGHTDFVSCIAF+SNL+CSHGFL SGSGDSTVRLWDILSGS
Sbjct: 181 VTMFPTKPVNEAHEIQSFCLGHTDFVSCIAFVSNLQCSHGFLISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LLDTCEIGD                                                   
Sbjct: 241 LLDTCEIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLSTSCSTGYLWMVTGVS+LHNLGHS+LA VKV+SGFNKDN  S
Sbjct: 301 SVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLAHVKVMSGFNKDN--S 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             N+VS++EDDAIPGG KLLEKLQGG+TFDEKVFLAAAEAVKTAMSNLLTKKQYS EKRD
Sbjct: 361 TANDVSVVEDDAIPGGTKLLEKLQGGITFDEKVFLAAAEAVKTAMSNLLTKKQYSIEKRD 420

[Close]

BLAST of HG10022827 vs. NCBI nr
Match: XP_022954128.1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita moschata] >XP_022954129.1 tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita moschata])

HSP 1 Score: 671.8 bits (1732), Expect = 3.5e-189
Identity = 349/433 (80.60%), Postives = 360/433 (83.14%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVE+APALIAVHP EKSVAVAVGSDLRVFDLVGNCP+SL+DESGG Y
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLIDESGGSY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGS G+LFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISPNG+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS LEC HGFL SGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCE+GD                                                   
Sbjct: 241 LLATCEVGDKVELLESKEREGCHSAVTSICTITDGALVAVAIQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLS+S ST YLWMVTGVS LHNLGH++LARVKVISGFNKDNP+ 
Sbjct: 301 SVVKVVSTSEETFIPTSLSSSFSTRYLWMVTGVSGLHNLGHASLARVKVISGFNKDNPNF 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             NE+SILEDDAIPGG KLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD
Sbjct: 361 TENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 420

[Close]

BLAST of HG10022827 vs. NCBI nr
Match: KAG7014455.1 (F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 354/462 (76.62%), Postives = 362/462 (78.35%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVE+APALIAVHP EKSVAVAVGSDLRVFDLVGNCP+SLVDESGG Y
Sbjct: 408 MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLVDESGGSY 467

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGS G+LFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISPNG+HVCF
Sbjct: 468 HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 527

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR
Sbjct: 528 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 587

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS LEC HGFL SGSGDSTVRLWDILSGS
Sbjct: 588 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 647

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCEIGD                                                   
Sbjct: 648 LLATCEIGDKVELLDSKEREGCHSAVTSICTIADGGLVAVAIQRQFSHLCSSSLVESYHR 707

Query: 301 --------------------------------KVVSFSEETFIPTSLSTSCSTGYLWMVT 360
                                           KVVS SEETFIPTSLS+SCST YLWMVT
Sbjct: 708 IFYLIYFIIFLCSLHGIILLNCDLSNHTLSVVKVVSISEETFIPTSLSSSCSTRYLWMVT 767

Query: 361 GVSTLHNLGHSALARVKVISGFNKDNPDSAVNEVSILEDDAIPGGMKLLEKLQGGVTFDE 380
           GVS LHNLGHS+LARVKVISGFNKDNP+S  NE+SILEDDAIPGG KLLEKLQGGVTFDE
Sbjct: 768 GVSGLHNLGHSSLARVKVISGFNKDNPNSTENELSILEDDAIPGGEKLLEKLQGGVTFDE 827

[Close]

BLAST of HG10022827 vs. ExPASy Swiss-Prot
Match: Q54UI3 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Dictyostelium discoideum OX=44689 GN=wdr4 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.9e-21
Identity = 71/249 (28.51%), Postives = 126/249 (50.60%), Query Frame = 0


Query: 14  DVEVAPALIAVHPTEKS-VAVAVGSDLRVFDLVGNCPISLVDESGGPYHKDSIRTICYGS 73
           +VE  P  I  H ++ + +A  + + L +FD   N    L +   G  H   IR+I +  
Sbjct: 17  NVEKVPISIITHSSDSNFIAYRLNNKLNIFD---NKLNKLGELESGSQHTGIIRSIEFTK 76

Query: 74  KGKLFVSAGDDKTVKIW-STESWRCIYNVVSEKRVSAAAISPNGLHVCFADKFGVIWVVD 133
             +  +++  DK +KIW +T +++ I ++ + K++  + ++ +   +  +DK G ++   
Sbjct: 77  NNQSLITSSSDKFLKIWDTTNNFKNIKSINTNKKIICSILNKDDSEILVSDKCGDVFKYS 136

Query: 134 L-DGGVDENQPLANKKAVS---------LLSHYCSIITSLEFSLDGRFLVSADRDFKIRV 193
           L D   ++ +   +K A           +  HY SI+  ++FS    +L+SADRD KIRV
Sbjct: 137 LIDDSKNKIEVSGDKSAKHDEKESDKNLVFGHYSSIV-DIKFSPCFNYLLSADRDEKIRV 196

Query: 194 TTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGSL 251
           + +P        +I+SFCLGHT +V+ I  +   +     L SGSGD T++LW+   G  
Sbjct: 197 SHYP-----NCFDIESFCLGHTKYVTEILLVPGRD---DLLISGSGDGTIKLWNWKQGKC 253

[Close]

BLAST of HG10022827 vs. ExPASy Swiss-Prot
Match: Q7ZY78 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus laevis OX=8355 GN=wdr4 PE=2 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 3.0e-18
Identity = 69/234 (29.49%), Postives = 112/234 (47.86%), Query Frame = 0


Query: 15  VEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGP--------YHKDSIR 74
           + V P  +A+    + +   VGS+   F L  +C + L  +S  P        +  D I 
Sbjct: 2   LRVGPGSLAITGGSRLLGHRVGSECCPFHL--DCSL-LEKQSAAPGQEGSADSHGSDKIL 61

Query: 75  TICYGSKGKLFVSAGDDKTVKIWSTE-SWRCIYNVVSEKRVSAAAISPNGLHVCFADKFG 134
              +   G+ F    D+K + ++ T+ +W  I      +R +A   SP G H+  ADK G
Sbjct: 62  AAAFSPSGEYFALTDDNKRLVLFRTKPAWEKISVRWVSRRCTALTFSPCGNHILVADKSG 121

Query: 135 VIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIRVTTFP 194
            ++   +   +++ +         L   + S++  +  SLDG+ +++ DRD KIRV+ + 
Sbjct: 122 DVFSFSVPRALEQGR---------LELGHLSMLLDVTVSLDGKHIITCDRDEKIRVSCW- 181

Query: 195 TKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSG 240
                  H I SFCLGHT+FVS +  +   E     L SGSGD T+RLW+  SG
Sbjct: 182 ----GAPHVIMSFCLGHTEFVSQLLPLPGQE---KLLLSGSGDGTLRLWEYESG 215

[Close]

BLAST of HG10022827 vs. ExPASy Swiss-Prot
Match: Q5FVB6 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus tropicalis OX=8364 GN=wdr4 PE=2 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 2.6e-17
Identity = 59/182 (32.42%), Postives = 90/182 (49.45%), Query Frame = 0


Query: 59  PYHKDSIRTICYGSKGKLFVSAGDDKTVKIWSTE-SWRCIYNVVSEKRVSAAAISPNGLH 118
           P   D I    +   G+ F    D+K + ++ T+ +W  I      +R +A   SP G H
Sbjct: 51  PQGSDKILAAAFSPSGEYFALTDDNKRLILFQTKPAWETISVRWVSRRCTALTFSPCGAH 110

Query: 119 VCFADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDF 178
           +  ADK G ++   +           +++A  L   + S++  +  S DG  +++ DRD 
Sbjct: 111 ILVADKSGDVFSFSV---------RRSREAGRLELGHLSMLLDVAVSPDGNHIITCDRDE 170

Query: 179 KIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDIL 238
           KIRV+ +     +  H I SFCLGHT+FVS +     L  +   L SGSGD T+RLWD  
Sbjct: 171 KIRVSRW-----DSPHVIMSFCLGHTEFVSQLL---PLPGADKLLLSGSGDGTLRLWDYE 215

Query: 239 SG 240
           SG
Sbjct: 231 SG 215

[Close]

BLAST of HG10022827 vs. ExPASy Swiss-Prot
Match: Q9EP82 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Mus musculus OX=10090 GN=Wdr4 PE=1 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 7.5e-17
Identity = 62/189 (32.80%), Postives = 96/189 (50.79%), Query Frame = 0


Query: 63  DSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCFAD 122
           DSI    +   G+ F    D K + ++ T+ W+C+   +  +R +A   + +   V  AD
Sbjct: 64  DSILASTFSKSGRYFALTDDSKRLILFRTKPWQCLSVRMVVRRCTALTFTASEDRVLVAD 123

Query: 123 KFGVIW---VVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKI 182
           K G ++   V++ DG               L   + S++  +  S D +F+++ADRD KI
Sbjct: 124 KSGDVYSFSVLEPDG------------CGRLELGHLSMLLDVAVSPDDQFVLTADRDEKI 183

Query: 183 RVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSH-GFLSSGSGDSTVRLWDILS 242
           RV ++   P    H I+SFCLGHT+FVS I  +     SH   L S SGD T+RLW+  S
Sbjct: 184 RV-SWAAAP----HSIESFCLGHTEFVSRILVVP----SHPELLLSSSGDGTLRLWEYRS 231

Query: 243 GSLLDTCEI 248
           G  L  C++
Sbjct: 244 GRQLQCCDL 231

[Close]

BLAST of HG10022827 vs. ExPASy Swiss-Prot
Match: A7E3S5 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Bos taurus OX=9913 GN=WDR4 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 9.8e-17
Identity = 63/198 (31.82%), Postives = 96/198 (48.48%), Query Frame = 0


Query: 55  ESGGPYHK--DSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAIS 114
           E G P  +  D++    +   G  FV   D K + ++ T  W+C+      +R +A   +
Sbjct: 53  EDGQPVDQGSDTVLASTFSKSGSYFVLTDDSKRLILFRTNPWQCLSVRTVVRRCTALTFT 112

Query: 115 PNGLHVCFADKFGVIW---VVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRF 174
            +   +  ADK G ++   V++  GG              L   + S++  +  S D RF
Sbjct: 113 ASEEKILVADKSGDVYSFSVLEPHGG------------GRLELGHLSMLLDVAVSPDDRF 172

Query: 175 LVSADRDFKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDS 234
           +++ADRD KIRV ++   P    H I+SFCLGHT+FVS I  + N       L S SGD 
Sbjct: 173 VLTADRDEKIRV-SWAAAP----HSIESFCLGHTEFVSRIFVVPN---HPELLLSSSGDC 230

Query: 235 TVRLWDILSGSLLDTCEI 248
           T+RLW+  SG  L  C +
Sbjct: 233 TLRLWEYRSGRELHCCPL 230

[Close]

BLAST of HG10022827 vs. ExPASy TrEMBL
Match: A0A0A0KAG0 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_7G387800 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 7.6e-190
Identity = 347/433 (80.14%), Postives = 363/433 (83.83%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PV+EGDTNKDVEVAPALIA+HP+E SVAVAVGSDLRVFDLVGNC +SLVDESG PY
Sbjct: 1   MEETPVDEGDTNKDVEVAPALIALHPSENSVAVAVGSDLRVFDLVGNCAVSLVDESGPPY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGSKG+LF+SAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFISAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VT FPTKPVNEAHEIQSFCLGHTDFVSCIAF+SNL+CSHGFL SGSGDSTVRLWDILSGS
Sbjct: 181 VTMFPTKPVNEAHEIQSFCLGHTDFVSCIAFVSNLQCSHGFLISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LLDTCEIGD                                                   
Sbjct: 241 LLDTCEIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLSTSCSTGYLWMVTGVS+LHNLGHS+LA VKV+SGFNKDN  S
Sbjct: 301 SVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLAHVKVMSGFNKDN--S 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             N+VS++EDDAIPGG KLLEKLQGG+TFDEKVFLAAAEAVKTAMSNLLTKKQYS EKRD
Sbjct: 361 TANDVSVVEDDAIPGGTKLLEKLQGGITFDEKVFLAAAEAVKTAMSNLLTKKQYSIEKRD 420

[Close]

BLAST of HG10022827 vs. ExPASy TrEMBL
Match: A0A6J1GQ89 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111456486 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.7e-189
Identity = 349/433 (80.60%), Postives = 360/433 (83.14%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVE+APALIAVHP EKSVAVAVGSDLRVFDLVGNCP+SL+DESGG Y
Sbjct: 1   MEETPVEEGDTNKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVGNCPVSLIDESGGSY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGS G+LFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISPNG+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS LEC HGFL SGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCE+GD                                                   
Sbjct: 241 LLATCEVGDKVELLESKEREGCHSAVTSICTITDGALVAVAIQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLS+S ST YLWMVTGVS LHNLGH++LARVKVISGFNKDNP+ 
Sbjct: 301 SVVKVVSTSEETFIPTSLSSSFSTRYLWMVTGVSGLHNLGHASLARVKVISGFNKDNPNF 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             NE+SILEDDAIPGG KLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD
Sbjct: 361 TENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 420

[Close]

BLAST of HG10022827 vs. ExPASy TrEMBL
Match: A0A6J1JND1 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111488451 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.5e-188
Identity = 349/433 (80.60%), Postives = 358/433 (82.68%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PV EGDT KDVE+APALIAVHP EKSVAVAVGSDLRVFDLV NCP+SL+DESGG Y
Sbjct: 1   MEETPV-EGDTKKDVEIAPALIAVHPAEKSVAVAVGSDLRVFDLVDNCPVSLIDESGGSY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGS G+LFVSAGDDKTVKIWSTESWRCIYNV SEKRVSAAAISPNG+HVCF
Sbjct: 61  HKDSIRTICYGSNGQLFVSAGDDKTVKIWSTESWRCIYNVGSEKRVSAAAISPNGMHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL NKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVNKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFIS LEC HGFL SGSGDSTVRLWDILSGS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISKLECPHGFLISGSGDSTVRLWDILSGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LL TCEIGD                                                   
Sbjct: 241 LLATCEIGDKVELLESKEREGCHSAVTSICTIADGTLVAVAIQSLHGIILLNCDLSNHSL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLS+SCST YLWMVTGVS LHNLGHS+L RVKVISGFNKDNP+S
Sbjct: 301 SVVKVVSISEETFIPTSLSSSCSTRYLWMVTGVSGLHNLGHSSLVRVKVISGFNKDNPNS 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             NE+SILEDDAIPGG KLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD
Sbjct: 361 TENELSILEDDAIPGGEKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 420

[Close]

BLAST of HG10022827 vs. ExPASy TrEMBL
Match: A0A5D3DRZ8 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001450 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.6e-187
Identity = 346/433 (79.91%), Postives = 360/433 (83.14%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVEVAPALIA+HPTE SVAVAVGSDLRVFDLVGNC + LVDESG PY
Sbjct: 1   MEETPVEEGDTNKDVEVAPALIALHPTENSVAVAVGSDLRVFDLVGNCAVFLVDESGVPY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGSKG+LFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL +KKA+SLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVSKKAMSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEA EIQSFCLGHTDFVSCIAFISNLECSHGFL SGSGDSTVRLWDIL GS
Sbjct: 181 VTTFPTKPVNEAREIQSFCLGHTDFVSCIAFISNLECSHGFLISGSGDSTVRLWDILCGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LLDTC+IGD                                                   
Sbjct: 241 LLDTCDIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLSTSCSTGYLWMVTGVS+LHNLGHS+LARVKV+SGFNKDN   
Sbjct: 301 SVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLARVKVMSGFNKDN--Y 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             N+VS++EDDAIPG  KLLEKLQGGVTFD+KVFLAAAEAVKTAMSNLLTKKQYS EKRD
Sbjct: 361 TANDVSVVEDDAIPGETKLLEKLQGGVTFDDKVFLAAAEAVKTAMSNLLTKKQYSMEKRD 420

[Close]

BLAST of HG10022827 vs. ExPASy TrEMBL
Match: A0A1S3BRA3 (tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo OX=3656 GN=LOC103492652 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.6e-187
Identity = 346/433 (79.91%), Postives = 360/433 (83.14%), Query Frame = 0


Query: 1   MEEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPY 60
           MEE PVEEGDTNKDVEVAPALIA+HPTE SVAVAVGSDLRVFDLVGNC + LVDESG PY
Sbjct: 1   MEETPVEEGDTNKDVEVAPALIALHPTENSVAVAVGSDLRVFDLVGNCAVFLVDESGVPY 60

Query: 61  HKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCF 120
           HKDSIRTICYGSKG+LFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAIS NGLHVCF
Sbjct: 61  HKDSIRTICYGSKGQLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISSNGLHVCF 120

Query: 121 ADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIR 180
           ADKFGVIWVVDLDGGVD NQPL +KKA+SLLSHYCSIITSL+FSLDGRFLVSADRDFKIR
Sbjct: 121 ADKFGVIWVVDLDGGVDGNQPLVSKKAMSLLSHYCSIITSLKFSLDGRFLVSADRDFKIR 180

Query: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGS 240
           VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFL SGSGDSTVRLWDIL GS
Sbjct: 181 VTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLISGSGDSTVRLWDILCGS 240

Query: 241 LLDTCEIGD--------------------------------------------------- 300
           LLDTC+IGD                                                   
Sbjct: 241 LLDTCDIGDKVELLDSKEREGCHSAVTSLCTIADGALVAVAVQSLHGIILLNCDLSNHTL 300

Query: 301 ---KVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 360
              KVVS SEETFIPTSLSTSCSTGYLWMVTGVS+LHNLGHS+LARVKV+SGFNKDN   
Sbjct: 301 SVVKVVSISEETFIPTSLSTSCSTGYLWMVTGVSSLHNLGHSSLARVKVMSGFNKDN--Y 360

Query: 361 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 380
             N+VS++EDDAIPG  KLLEKLQGGVTFD+KVFLAAAEAVKTAMSNLLTKKQYS EKRD
Sbjct: 361 TANDVSVVEDDAIPGETKLLEKLQGGVTFDDKVFLAAAEAVKTAMSNLLTKKQYSMEKRD 420

[Close]

BLAST of HG10022827 vs. TAIR 10
Match: AT1G03110.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 451.1 bits (1159), Expect = 9.1e-127
Identity = 239/430 (55.58%), Postives = 291/430 (67.67%), Query Frame = 0


Query: 2   EEMPVEEGDTNKDVEVAPALIAVHPTEKSVAVAVGSDLRVFDLVGNCPISLVDESGGPYH 61
           EE  +EE +    +EVAPALI+VHP++KSVAVAVGSDLR+FDL+ NCP+SLVDES GP  
Sbjct: 3   EESHIEEVEIQNKLEVAPALISVHPSQKSVAVAVGSDLRIFDLIENCPVSLVDESDGPIR 62

Query: 62  KDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNVVSEKRVSAAAISPNGLHVCFA 121
           K+SIR I Y + GKLFVSAGDDK VKIWS +SWRC+  V SEKRVSA AIS +  HVC+A
Sbjct: 63  KESIRAIRYSTSGKLFVSAGDDKLVKIWSADSWRCLNTVCSEKRVSAVAISSDDSHVCYA 122

Query: 122 DKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRDFKIRV 181
           DKFGV+WV++LD G+++ + L +KK   LLSHYCSIITSLEFS DGR+++SADRDFKIRV
Sbjct: 123 DKFGVVWVIELD-GINDGKTLPSKKGALLLSHYCSIITSLEFSPDGRYILSADRDFKIRV 182

Query: 182 TTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLWDILSGSL 241
           T FP KP+  AHEIQSFCLGH++F++C AF+S  E + G+L SGSGDSTVRLWDI SGSL
Sbjct: 183 TVFPKKPLEGAHEIQSFCLGHSEFITCTAFVSTPELTQGYLMSGSGDSTVRLWDITSGSL 242

Query: 242 LDTCEIG----------------------------------------------------- 301
           LDTCE+                                                      
Sbjct: 243 LDTCEVSTVAGHAESNENESPTQVTVTDICAIPNSSLAAVAIQSFQGIFLLSCDLTAHTL 302

Query: 302 --DKVVSFSEETFIPTSLSTSCSTGYLWMVTGVSTLHNLGHSALARVKVISGFNKDNPDS 361
              KV+    E+FIPTS++ S S+  LWMV+G S L    H   +RV+VIS    ++   
Sbjct: 303 SITKVIKIPGESFIPTSIAVSASSRLLWMVSGASNLPGSNHPGFSRVRVISCLETESS-- 362

Query: 362 AVNEVSILEDDAIPGGMKLLEKLQGGVTFDEKVFLAAAEAVKTAMSNLLTKKQYSTEKRD 377
                SILED+ IPGG KLLE+LQG VT +E V  AAAEAV+ AMS+LL KKQYS EKR+
Sbjct: 363 -----SILEDEQIPGGTKLLEQLQGKVTIEESVMSAAAEAVRAAMSSLLMKKQYSEEKRE 422

[Close]

BLAST of HG10022827 vs. TAIR 10
Match: AT5G50120.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.6e-12
Identity = 52/180 (28.89%), Postives = 88/180 (48.89%), Query Frame = 0


Query: 60  YHKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCI--YNVVSEKRVSAAAISPNG-L 119
           +H D++  +     G L  S   D+T+KIW T  ++C+  +    +  ++A A+S NG +
Sbjct: 163 HHVDAVSGLALSRDGTLLYSVSWDRTLKIWRTTDFKCLESFTNAHDDAINAVALSENGDI 222

Query: 120 HVCFADKFGVIWVVDLDGGVDENQPLANKKA-VSLLSHYCSIITSLEFS-LDGRFLVSAD 179
           +   +D+   +W  +++   +EN     K + V++LS + S I +L  S  +G  L S  
Sbjct: 223 YTGSSDQRIKVWRKNIN---EENVKKKRKHSLVAILSEHNSGINALALSGTNGSLLHSGG 282

Query: 180 RDFKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLW 235
            D  I V     +       +     GHT+ V C+A +S++ C      SGS D TVRLW
Sbjct: 283 SDGSILVW---ERDDGGDIVVVGMLRGHTESVLCLAVVSDILC------SGSADKTVRLW 330

[Close]

BLAST of HG10022827 vs. TAIR 10
Match: AT4G29830.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.2e-11
Identity = 66/251 (26.29%), Postives = 106/251 (42.23%), Query Frame = 0


Query: 11  TNKDVEVAPALIAVHPTE-KSVAVAVGSDLRVFDLVGNCPISLVDESGGPYHKDSIRTIC 70
           TN    +  A +A HP+   + + ++ S +RVFD+  N  I++++          +  + 
Sbjct: 55  TNTGHSLGVAALAAHPSGIIAASSSIDSFVRVFDVDTNATIAVLEAP-----PSEVWGMQ 114

Query: 71  YGSKGK-LFVSAGDDKTVKIWSTESWRCIYNV--------------VSEKRVSAAAISPN 130
           +  KG  L V+ G   +VK+W T SWR I  +               S+K V + A SPN
Sbjct: 115 FEPKGTILAVAGGSSASVKLWDTASWRLISTLSIPRPDAPKPSDKTSSKKFVLSVAWSPN 174

Query: 131 GLHVCFADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFS-LDGRFLVSA 190
           G  +      G I V D+D            K +  L  +   + SL FS +D R L S 
Sbjct: 175 GKRLACGSMDGTICVFDVD----------RSKLLHQLEGHNMPVRSLVFSPVDPRVLFSG 234

Query: 191 DRDFKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRL 245
             D  + +         E   +     GHT +V  +    +     G +++GS D TVRL
Sbjct: 235 SDDGHVNMHD------AEGKTLLGSMSGHTSWVLSV----DASPDGGAIATGSSDRTVRL 280

[Close]

BLAST of HG10022827 vs. TAIR 10
Match: AT1G24130.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-10
Identity = 46/180 (25.56%), Postives = 81/180 (45.00%), Query Frame = 0


Query: 60  YHKDSIRTICYGSKGKLFVSAGDDKTVKIWSTESWRCIYNV--VSEKRVSAAAISPNG-L 119
           +H D++ ++     G L  SA  D++ KIW T  ++C+ ++    +  ++A  +S +G +
Sbjct: 190 HHVDAVSSLALSQDGSLLYSASWDRSFKIWRTSDFKCLDSIEKAHDDAINAIVVSKDGFV 249

Query: 120 HVCFADKFGVIWVVDLDGGVDENQPLANKKAVSLLSHYCSIITSLEFSLDGRFLVSADRD 179
           +   ADK   +W          N+       V+ L+ + S + +L  S DG+ L S   D
Sbjct: 250 YTGSADKKIKVW----------NKKDKKHSLVATLTKHLSAVNALAISEDGKVLYSGACD 309

Query: 180 FKIRV-TTFPTKPVNEAH-EIQSFCLGHTDFVSCIAFISNLECSHGFLSSGSGDSTVRLW 235
             I V          E H  +     GH   + C+A  S+L      + SGS D ++R+W
Sbjct: 310 RSILVWERLINGDDEELHMSVVGALRGHRKAIMCLAVASDL------VLSGSADKSLRVW 353

[Close]

BLAST of HG10022827 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.9e-09
Identity = 36/94 (38.30%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 151 LSHYCSIITSLEFSLDGRFLVSADRDFKIRVTTFPTKPVNEAHEIQSFCLGHTDFVSCIA 210
           L+ +   ++S++FS DGR L SA  D  IR  T  T     A  +Q F  GH + +S +A
Sbjct: 20  LTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPIAEPVQEF-TGHENGISDVA 79

Query: 211 FISNLECSHGFLSSGSGDSTVRLWDILSGSLLDT 245
           F S+      F+ S S D T++LWD+ +GSL+ T
Sbjct: 80  FSSDAR----FIVSASDDKTLKLWDVETGSLIKT 108

The following BLAST results are available for this feature:

BLAST of HG10022827 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023549061.1	2.2e-191	81.29	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita pep...	[more]
XP_038899582.1	1.1e-190	81.76	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Benincasa his...	[more]
XP_004146976.1	1.6e-189	80.14	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucumis sativ...	[more]
XP_022954128.1	3.5e-189	80.60	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 [Cucurbita mos...	[more]
KAG7014455.1	1.3e-188	76.62	F-box/kelch-repeat protein SKIP30 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of HG10022827 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54UI3	2.9e-21	28.51	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Dictyosteli...	[more]
Q7ZY78	3.0e-18	29.49	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus lae...	[more]
Q5FVB6	2.6e-17	32.42	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit wdr4 OS=Xenopus tro...	[more]
Q9EP82	7.5e-17	32.80	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Mus musculu...	[more]
A7E3S5	9.8e-17	31.82	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 OS=Bos taurus ...	[more]

BLAST of HG10022827 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAG0	7.6e-190	80.14	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis sativus ...	[more]
A0A6J1GQ89	1.7e-189	80.60	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita moscha...	[more]
A0A6J1JND1	2.5e-188	80.60	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucurbita maxima...	[more]
A0A5D3DRZ8	4.6e-187	79.91	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo var...	[more]
A0A1S3BRA3	4.6e-187	79.91	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit OS=Cucumis melo OX=...	[more]

BLAST of HG10022827 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03110.1	9.1e-127	55.58	Transducin/WD40 repeat-like superfamily protein	[more]
AT5G50120.1	2.6e-12	28.89	Transducin/WD40 repeat-like superfamily protein	[more]
AT4G29830.1	2.2e-11	26.29	Transducin/WD40 repeat-like superfamily protein	[more]
AT1G24130.1	3.1e-10	25.56	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G49660.1	5.9e-09	38.30	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 45..90 e-value: 7.8E-4 score: 28.7 coord: 93..131 e-value: 120.0 score: 3.9 coord: 191..235 e-value: 6.2E-4 score: 29.1 coord: 144..183 e-value: 0.36 score: 19.8
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 61..90 e-value: 8.5E-4 score: 20.1 coord: 152..181 e-value: 0.022 score: 15.6 coord: 199..235 e-value: 8.6E-4 score: 20.1
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 61..99 score: 12.045688
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 199..244 score: 12.045688
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 19..102 e-value: 7.1E-12 score: 47.2
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 103..303 e-value: 2.0E-21 score: 78.8
IPR028884	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit	PANTHER	PTHR16288	WD40 REPEAT PROTEIN 4	coord: 250..376 coord: 20..247
IPR028884	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit	HAMAP	MF_03056	TRM82	coord: 28..371 score: 20.78516
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 222..236
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 199..236 score: 9.151057
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 58..93 score: 9.625601
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 22..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022827.1	HG10022827.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0036265	RNA (guanine-N7)-methylation
biological_process	GO:0106004	tRNA (guanine-N7)-methylation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
cellular_component	GO:0043527	tRNA methyltransferase complex
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008176	tRNA (guanine-N7-)-methyltransferase activity