HG10022770 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022770 vs. NCBI nr
Match: XP_038899504.1 (protein DOWNY MILDEW RESISTANCE 6-like [Benincasa hispida])

HSP 1 Score: 477.6 bits (1228), Expect = 8.7e-131
Identity = 220/333 (66.07%), Postives = 271/333 (81.38%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+QMK+ SNW  VE+VPE YVFP EKRP VAVK TI V+D  T +R  L Q ILD  ++ 
Sbjct: 5   MSQMKVVSNWMDVESVPENYVFPSEKRPTVAVKTTISVLDLATDDRTFLFQNILDITRDL 64

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVH 120
           GF QVINHG+SK+LI +TM +FKEF+AMS+E+KERECSKDPNKS KF+ S+ NY  E+ H
Sbjct: 65  GFFQVINHGVSKELINKTMMVFKEFHAMSSEEKERECSKDPNKSCKFYTSSVNYETEQAH 124

Query: 121 NWREVSDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYF 180
           +WR+VS F C+PL+KY+HFWP+KP  FREVVGEYC+EMRK A KILEVISEGLG+G+ YF
Sbjct: 125 SWRDVSQFDCHPLEKYIHFWPEKPLNFREVVGEYCIEMRKLACKILEVISEGLGLGRDYF 184

Query: 181 ENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDPL 240
           ++EMS +  L V+HYPPCPNPSLT GL PHCDP+ ITILFQD+NGLQ+FK+GQWIDVDP+
Sbjct: 185 KSEMSEHSSLLVNHYPPCPNPSLTLGLFPHCDPNFITILFQDINGLQVFKNGQWIDVDPI 244

Query: 241 DDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEA 300
           DDAL++NFGY LE+ISNG  KATEHRV+TN  T R +L Y +FP+ND  IEPAK+LINE 
Sbjct: 245 DDALIVNFGYFLEIISNGNFKATEHRVMTNTKTYRQTLVYLVFPKNDATIEPAKNLINEV 304

Query: 301 NPPHYRSIKFKDFIRSFFSTPNIDREIVMKYIS 334
           +PP YRSI  K+F+  +F  P  DRE+V+KY++
Sbjct: 305 DPPQYRSINCKEFMGEYFYMPR-DREVVIKYLT 336

BLAST of HG10022770 vs. NCBI nr
Match: XP_038899650.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 456.1 bits (1172), Expect = 2.7e-124
Identity = 217/333 (65.17%), Postives = 264/333 (79.28%), Query Frame = 0

Query: 4   MKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFL 63
           M   SNW+GV++VP+ YVFPPEKRP VAVK TIPVIDF T++RALL Q+ILD  QEFGF 
Sbjct: 1   MNFLSNWEGVKSVPKNYVFPPEKRPCVAVKKTIPVIDFATNDRALLFQRILDVTQEFGFF 60

Query: 64  QVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWR 123
           QVINHG+SKKLI+ET+K  KEFN+MS EDK+RECSKDP+   K +  +QN  NE+ H WR
Sbjct: 61  QVINHGVSKKLIEETVKTLKEFNSMSPEDKKRECSKDPDYC-KVYPGSQNSANEQTHFWR 120

Query: 124 EVSDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENE 183
           +   F C+PL+KY+HFWP KPP+FREVVGEYCV MRKF  ++L+VISEGL +G+GY  +E
Sbjct: 121 DSMSFNCHPLEKYIHFWPDKPPEFREVVGEYCVAMRKFVLELLDVISEGLKLGRGYLGDE 180

Query: 184 MSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDPLDDA 243
           MS NP + V +YPPCP+PSLT GL  HCD  +ITILFQDVNGLQ+ KDG+WI  DP+DDA
Sbjct: 181 MSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLITILFQDVNGLQILKDGEWIAADPIDDA 240

Query: 244 LLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEANPP 303
           LLINFGYVLE+ISNGKLKA EHR +TNM  SR SL Y + PEND+ IEPAK LINE NP 
Sbjct: 241 LLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQSLIYFVCPENDVTIEPAKCLINETNPL 300

Query: 304 HYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQ 337
            YR+IKF+DF   ++S    ++E +MKY+S N+
Sbjct: 301 RYRTIKFEDFRGKYYSV-LANKEEIMKYMSLNK 331

BLAST of HG10022770 vs. NCBI nr
Match: XP_008451257.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 455.7 bits (1171), Expect = 3.5e-124
Identity = 221/339 (65.19%), Postives = 264/339 (77.88%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+Q K  SNWKGVE+VPETYV+PPEKR GV+VK TIPVID+   +R LL +K+ D  QEF
Sbjct: 2   MSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQEF 61

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDP-NKSNKFFRSTQNYTNEEV 120
           GF+QVINHG+ K L++ETMKI KEF+AMS EDKERECSKDP +KS + + S+QNY  E++
Sbjct: 62  GFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQI 121

Query: 121 HNWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKG 180
           H W++     C+P LD+ V FWPQ PPKFREVV  Y +++ KFA +ILEV+SEGLG+ KG
Sbjct: 122 HLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKG 181

Query: 181 YFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVD 240
           Y   EMS NP L V HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDGQWIDVD
Sbjct: 182 YLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVD 241

Query: 241 PLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLIN 300
           PL +A L+N GYVLE+ISNGK+KA EHRVVTN  TSR SL Y ++P ND+ IEP+K  IN
Sbjct: 242 PLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN 301

Query: 301 EANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQP 338
           EANPPHY S++FKDFI+   S P  DRE  +K+ISFNQP
Sbjct: 302 EANPPHYLSLEFKDFIKKLMSKPP-DRETALKFISFNQP 339

BLAST of HG10022770 vs. NCBI nr
Match: KAA0059649.1 (hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK08181.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 454.9 bits (1169), Expect = 6.0e-124
Identity = 219/339 (64.60%), Postives = 265/339 (78.17%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+Q K  SNWKGVE+VPETYV+PPEKR G++VK TIPVID+   +RALL +K+ D  +EF
Sbjct: 2   MSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTREF 61

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDP-NKSNKFFRSTQNYTNEEV 120
           GF+QVINHG+ K L++ETMK+ KEF+AMS EDKERECSKDP +KS + + S+QNY  E++
Sbjct: 62  GFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQI 121

Query: 121 HNWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKG 180
           H W++     C+P LD+ V FWPQ PPKFREVV  Y +++ KFA +ILEV+SEGLG+ KG
Sbjct: 122 HLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKG 181

Query: 181 YFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVD 240
           Y   EMS NP L V HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDGQWIDVD
Sbjct: 182 YLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVD 241

Query: 241 PLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLIN 300
           PL +A L+N GYVLE+ISNGK+KA EHRVVTN  TSR SL Y ++P ND+ IEP+K  IN
Sbjct: 242 PLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN 301

Query: 301 EANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQP 338
           EANPPHY S++FKDFI+   S P  DRE  +K+ISFNQP
Sbjct: 302 EANPPHYLSLEFKDFIKKLMSKPP-DRETALKFISFNQP 339

BLAST of HG10022770 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 450.3 bits (1157), Expect = 1.5e-122
Identity = 208/335 (62.09%), Postives = 266/335 (79.40%), Query Frame = 0

Query: 5   KIESNWKGVETVPETYVFPPEKRPG---VAVKMTIPVIDFGTHNRALLCQKILDTAQEFG 64
           K  SNW+ VE+VPE+YV+PPEKRPG   V +   IPVID    +R LL +KILD ++EFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 65  FLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHN 124
           F Q+INHG+SKK+ +ETM+IFKEF+AMS  +K +ECSKDPN+S + + S++NYT E+VH 
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 125 WREVSDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFE 184
           WR+   F C+PL+KYVHFWPQKPPK+REVVG YC+ MRK   +ILE++SEGLG+GKGYF 
Sbjct: 127 WRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 186

Query: 185 NEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDPLD 244
            EMS NPLL V+HYPPCPNPSLT GL  HCDP +ITILFQDVNGLQ+ KDGQWI V P+D
Sbjct: 187 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 246

Query: 245 DALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEAN 304
           +A ++N G+VL++I+NGKL+A EHR VTN  TSR SLTY ++P+++ ++EPAK +INEAN
Sbjct: 247 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 306

Query: 305 PPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQ 337
           PP YRS+ FKDF R++     +D + VM+YI  NQ
Sbjct: 307 PPRYRSLNFKDFQRNYLPRA-VDTKAVMQYIETNQ 340

BLAST of HG10022770 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.0e-61
Identity = 130/321 (40.50%), Postives = 190/321 (59.19%), Query Frame = 0

Query: 15  TVPETYVFPPEKRP---GVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFLQVINHGIS 74
           T+PE YV P   RP    V+     P+ID  + +R+ L Q+I      FGF QVINHG++
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDFKCY 134
           K++I E + + +EF +MS E+K +  S DP K+ +   S  N   EEV+NWR+     CY
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 135 PLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEM-SGNPLL 194
           P+ KYV+ WP  PP F+E+V +Y  E+R+   KI E+ISE LG+ K Y +  +      +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 195 SVSHYPPCPNPSLTFGLIPHCDPDIITILFQD--VNGLQLFKDGQWIDVDPLDDALLINF 254
           +V++YPPCP P LT+GL  H DP+ +TIL QD  V GLQ+  DGQW  V+P  DA +IN 
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 255 GYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSL---INEANPPHY 314
           G  L+ +SNG  K+  HR VTN    R S+   + P +  ++ PAK L    ++   P Y
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVY 313

Query: 315 RSIKFKDFIRSFFSTPNIDRE 327
           +   + ++ + F+S  N+D+E
Sbjct: 314 KDFTYAEYYKKFWSR-NLDQE 332

BLAST of HG10022770 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-61
Identity = 130/325 (40.00%), Postives = 196/325 (60.31%), Query Frame = 0

Query: 8   SNWKGVETVPETYVFPPEKR--PGVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFLQV 67
           SNW   ++V E+++ P +KR    V V   +P+ID   H+  LL Q+I    Q+FG  QV
Sbjct: 6   SNW-STKSVSESFIAPLQKRAEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGLFQV 65

Query: 68  INHGISKKLIKETMKIFKEFNAMSTEDKER-----ECSK---DPNKSNKFFRSTQNYTNE 127
           INHG  ++L+ ETM++ KEF A+  E+KE+     E +K      +  K +   +  +NE
Sbjct: 66  INHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLSNE 125

Query: 128 EVHNWREVSDFKCYPLDK-YVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIG 187
           E   W++     C+PLD+  V+ WP+KP K+REVV +Y VE+RK   ++L+ I EGLG+ 
Sbjct: 126 EFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGLGLK 185

Query: 188 KGYFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQ--LFKDGQW 247
            GYF+NE+S   ++  ++YPPCP+PS T G   H D ++IT+L QD+ GLQ  + KD  W
Sbjct: 186 LGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKDATW 245

Query: 248 IDVDPLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAK 307
           I V P+  A ++N G  L++I+N K + + HRVVT+    R S+   I P+    IEPAK
Sbjct: 246 IAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIEPAK 305

Query: 308 SLINEANPPHYRSIKFKDFIRSFFS 320
            L+N+ NPP Y+   + +F   + S
Sbjct: 306 ELLNQDNPPLYKPYSYSEFADIYLS 328

BLAST of HG10022770 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 123/319 (38.56%), Postives = 185/319 (57.99%), Query Frame = 0

Query: 14  ETVPETYVFPPEKRPGVAVKMT---IPVIDFGTHNRALLCQKILDTAQEFGFLQVINHGI 73
           +T+P  YV P  +RP + + ++   IPV+D  + +RA +   + D  +  GF QV+NHGI
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDFKC 133
              LI   M++ +EF  +  E+K +  S DP K  +   S  N   E VHNWR+     C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHC 137

Query: 134 YPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEMSGNPL- 193
           YPL ++V  WP  PP F+E++G YC E+R+   ++ E ISE LG+  GY    +      
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 194 LSVSHYPPCPNPSLTFGLIPHCDPDIITILFQD--VNGLQLFKDGQWIDVDPLDDALLIN 253
           ++V++YP CP P LT+GL  H DP+ +TIL  D  V GLQ+  DG+WI V+P   AL+IN
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 254 FGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEANPPHYRS 313
            G  L+ +SNGK ++  HR V N    R S+   + P N + + PAK LI + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 314 IKFKDFIRSFFSTPNIDRE 327
             + ++ + F+S  N+D+E
Sbjct: 318 YTYDEYYKKFWSR-NLDQE 334

BLAST of HG10022770 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 222.2 bits (565), Expect = 8.6e-57
Identity = 112/307 (36.48%), Postives = 186/307 (60.59%), Query Frame = 0

Query: 15  TVPETYVFPPEK-RPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFLQVINHGISKK 74
           ++PE++VF  +K  P     +++P+ID     R  + + IL+  +E GF QV+NHG+SK+
Sbjct: 13  SLPESFVFASDKVPPATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHGVSKQ 72

Query: 75  LIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDFKC-YP 134
           ++++   + ++F  +   DK    S++ +K N+ F      T  E + WR+     C +P
Sbjct: 73  VMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLFSGATYDTGGEKY-WRDCLRLACPFP 132

Query: 135 LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEMS-GNPLLS 194
           +D  ++ WP  P   R+V+ ++  + R    ++L ++ EG+GI   YFE ++S GN +L+
Sbjct: 133 VDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGNVILN 192

Query: 195 VSHYPPCPNPSLTFGLIPHCDPDIITILFQD-VNGLQLFKDGQWIDVDPLDDALLINFGY 254
           ++HYP CPNP    G  PHCD ++IT+L    VNGL++   G WI VDP  +A ++NFG 
Sbjct: 193 INHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNFGQ 252

Query: 255 VLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEANPPHYRSIKF 314
            LE+++NG LK+ EHR +TN   +RTS+   I P  + +I PAK  +++ NPP YR+  F
Sbjct: 253 QLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTTMF 312

Query: 315 KDFIRSF 318
           +DF+R +
Sbjct: 313 RDFMRIY 317

BLAST of HG10022770 vs. ExPASy Swiss-Prot
Match: Q40061 (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.6e-55
Identity = 120/316 (37.97%), Postives = 186/316 (58.86%), Query Frame = 0

Query: 15  TVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFLQVINHGISKKL 74
           ++P++++ P ++       +++P+ID  +  R  + Q ILD+ +E+GF+QV+NHGIS+ +
Sbjct: 14  SIPDSFLLPADRLHPATTDVSLPIIDM-SRGRDEVRQAILDSGKEYGFIQVVNHGISEPM 73

Query: 75  IKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDFKCYPLD 134
           + E   +  EF  M  EDK    S+D ++ NK F  +   T  E + W +V +   YPL 
Sbjct: 74  LHEMYAVCHEFFDMPAEDKAEFFSEDRSERNKLFCGSAFETLGEKY-WIDVLEL-LYPLP 133

Query: 135 K-YVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEMS-GNPLLSV 194
                 WP KP   REVVG Y    R  A +IL ++ EGLG+   +F  ++S G  ++ +
Sbjct: 134 SGDTKDWPHKPQMLREVVGNYTSLARGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDI 193

Query: 195 SHYPPCPNPSLTFGLIPHCDPDIITILFQD-VNGLQLFKDGQWIDVDPLDDALLINFGYV 254
           ++YPP PNPS T GL PHCD D++T+L    V GL++   G WI V P+ ++L+INFG  
Sbjct: 194 NYYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWIKVQPVPNSLVINFGLQ 253

Query: 255 LEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEANPPHYRSIKFK 314
           LE+++NG LKA EHR  TN    R S+   I P +D ++ PA+  ++E NPP YR++   
Sbjct: 254 LEVVTNGYLKAVEHRAATNFAEPRLSVASFIVPADDCVVGPAEEFVSEDNPPRYRTLTVG 313

Query: 315 DFIRSFFSTPNIDREI 328
           +F R   +  N+D  I
Sbjct: 314 EFKRK-HNVVNLDSSI 325

BLAST of HG10022770 vs. ExPASy TrEMBL
Match: A0A1S3BRU2 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.7e-124
Identity = 221/339 (65.19%), Postives = 264/339 (77.88%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+Q K  SNWKGVE+VPETYV+PPEKR GV+VK TIPVID+   +R LL +K+ D  QEF
Sbjct: 2   MSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQEF 61

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDP-NKSNKFFRSTQNYTNEEV 120
           GF+QVINHG+ K L++ETMKI KEF+AMS EDKERECSKDP +KS + + S+QNY  E++
Sbjct: 62  GFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQI 121

Query: 121 HNWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKG 180
           H W++     C+P LD+ V FWPQ PPKFREVV  Y +++ KFA +ILEV+SEGLG+ KG
Sbjct: 122 HLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKG 181

Query: 181 YFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVD 240
           Y   EMS NP L V HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDGQWIDVD
Sbjct: 182 YLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVD 241

Query: 241 PLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLIN 300
           PL +A L+N GYVLE+ISNGK+KA EHRVVTN  TSR SL Y ++P ND+ IEP+K  IN
Sbjct: 242 PLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN 301

Query: 301 EANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQP 338
           EANPPHY S++FKDFI+   S P  DRE  +K+ISFNQP
Sbjct: 302 EANPPHYLSLEFKDFIKKLMSKPP-DRETALKFISFNQP 339

BLAST of HG10022770 vs. ExPASy TrEMBL
Match: A0A5A7V1J0 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00760 PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.9e-124
Identity = 219/339 (64.60%), Postives = 265/339 (78.17%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+Q K  SNWKGVE+VPETYV+PPEKR G++VK TIPVID+   +RALL +K+ D  +EF
Sbjct: 2   MSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTREF 61

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDP-NKSNKFFRSTQNYTNEEV 120
           GF+QVINHG+ K L++ETMK+ KEF+AMS EDKERECSKDP +KS + + S+QNY  E++
Sbjct: 62  GFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQI 121

Query: 121 HNWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKG 180
           H W++     C+P LD+ V FWPQ PPKFREVV  Y +++ KFA +ILEV+SEGLG+ KG
Sbjct: 122 HLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKG 181

Query: 181 YFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVD 240
           Y   EMS NP L V HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDGQWIDVD
Sbjct: 182 YLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVD 241

Query: 241 PLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLIN 300
           PL +A L+N GYVLE+ISNGK+KA EHRVVTN  TSR SL Y ++P ND+ IEP+K  IN
Sbjct: 242 PLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFIN 301

Query: 301 EANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQP 338
           EANPPHY S++FKDFI+   S P  DRE  +K+ISFNQP
Sbjct: 302 EANPPHYLSLEFKDFIKKLMSKPP-DRETALKFISFNQP 339

BLAST of HG10022770 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 7.2e-123
Identity = 208/335 (62.09%), Postives = 266/335 (79.40%), Query Frame = 0

Query: 5   KIESNWKGVETVPETYVFPPEKRPG---VAVKMTIPVIDFGTHNRALLCQKILDTAQEFG 64
           K  SNW+ VE+VPE+YV+PPEKRPG   V +   IPVID    +R LL +KILD ++EFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 65  FLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHN 124
           F Q+INHG+SKK+ +ETM+IFKEF+AMS  +K +ECSKDPN+S + + S++NYT E+VH 
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 125 WREVSDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFE 184
           WR+   F C+PL+KYVHFWPQKPPK+REVVG YC+ MRK   +ILE++SEGLG+GKGYF 
Sbjct: 127 WRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 186

Query: 185 NEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDPLD 244
            EMS NPLL V+HYPPCPNPSLT GL  HCDP +ITILFQDVNGLQ+ KDGQWI V P+D
Sbjct: 187 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 246

Query: 245 DALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEAN 304
           +A ++N G+VL++I+NGKL+A EHR VTN  TSR SLTY ++P+++ ++EPAK +INEAN
Sbjct: 247 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 306

Query: 305 PPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQ 337
           PP YRS+ FKDF R++     +D + VM+YI  NQ
Sbjct: 307 PPRYRSLNFKDFQRNYLPRA-VDTKAVMQYIETNQ 340

BLAST of HG10022770 vs. ExPASy TrEMBL
Match: A0A0A0K745 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.2e-122
Identity = 216/338 (63.91%), Postives = 260/338 (76.92%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M+Q K  SNWKG+ +VPETYV PPEKR GV+VK  IPVID  T +R LL +K+LD +QEF
Sbjct: 1   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQEF 60

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVH 120
           GF QVINHG+SK L++ETM++ +EF+AMS+EDKE ECSKDPNKS   + ST NY  EE+H
Sbjct: 61  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 120

Query: 121 NWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGY 180
            W++     CYP L+K V FWPQ PPKFREV+  Y V M+KF+ +ILEV+SEGLG+ K Y
Sbjct: 121 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 180

Query: 181 FENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDP 240
           F   MS NP   V HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDG+WIDVDP
Sbjct: 181 FSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 240

Query: 241 LDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINE 300
           L DA L+N GYV E+ISNGK+KA EHRVVTN  TSR SL Y ++PEN++ IEP+K  INE
Sbjct: 241 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 300

Query: 301 ANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQP 338
           ANPPHYR+++FKDF+    S  + DRE+ MK+ISFNQP
Sbjct: 301 ANPPHYRTLEFKDFVNKLRS-KHPDREMAMKFISFNQP 337

BLAST of HG10022770 vs. ExPASy TrEMBL
Match: A0A0A0K5H0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.8e-121
Identity = 214/337 (63.50%), Postives = 260/337 (77.15%), Query Frame = 0

Query: 1   MAQMKIESNWKGVETVPETYVFPPEKRPGVAVKMTIPVIDFGTHNRALLCQKILDTAQEF 60
           M++ K  SNW GV +VPETYV+PPEKR G++VK +IPVID+ + +RALL +++ D  QEF
Sbjct: 2   MSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDW-SDDRALLLKRVHDVTQEF 61

Query: 61  GFLQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVH 120
           GF+QVINHG+ K L++E MKI KEF+ MS EDKERECSKDP+KS K + S+QNY NE++H
Sbjct: 62  GFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQIH 121

Query: 121 NWREVSDFKCYP-LDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGY 180
            W++    +C+P LDK + FWPQ PPK REVV  Y V + KFAS+ILEV+SEGLG+ KGY
Sbjct: 122 LWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKGY 181

Query: 181 FENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFKDGQWIDVDP 240
           F  EMS NP     HYPPCPNPSLT GL  H DP +ITILFQDVNGLQ  KDGQWIDVDP
Sbjct: 182 FRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDP 241

Query: 241 LDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINE 300
           L DA L+N GYV E+ISNGKLKA EHRVVTN  TSR +L Y ++PEND+ IEP+K  INE
Sbjct: 242 LPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINE 301

Query: 301 ANPPHYRSIKFKDFIRSFFSTPNIDREIVMKYISFNQ 337
           ANPPHYRS++FKDF     S P  DR+  M++ISF+Q
Sbjct: 302 ANPPHYRSLEFKDFYNKLMSKPP-DRKTAMEFISFDQ 336

BLAST of HG10022770 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.4e-62
Identity = 130/321 (40.50%), Postives = 190/321 (59.19%), Query Frame = 0

Query: 15  TVPETYVFPPEKRP---GVAVKMTIPVIDFGTHNRALLCQKILDTAQEFGFLQVINHGIS 74
           T+PE YV P   RP    V+     P+ID  + +R+ L Q+I      FGF QVINHG++
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDFKCY 134
           K++I E + + +EF +MS E+K +  S DP K+ +   S  N   EEV+NWR+     CY
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 135 PLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEM-SGNPLL 194
           P+ KYV+ WP  PP F+E+V +Y  E+R+   KI E+ISE LG+ K Y +  +      +
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHM 193

Query: 195 SVSHYPPCPNPSLTFGLIPHCDPDIITILFQD--VNGLQLFKDGQWIDVDPLDDALLINF 254
           +V++YPPCP P LT+GL  H DP+ +TIL QD  V GLQ+  DGQW  V+P  DA +IN 
Sbjct: 194 AVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINI 253

Query: 255 GYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSL---INEANPPHY 314
           G  L+ +SNG  K+  HR VTN    R S+   + P +  ++ PAK L    ++   P Y
Sbjct: 254 GDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVY 313

Query: 315 RSIKFKDFIRSFFSTPNIDRE 327
           +   + ++ + F+S  N+D+E
Sbjct: 314 KDFTYAEYYKKFWSR-NLDQE 332

BLAST of HG10022770 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 208.8 bits (530), Expect = 7.0e-54
Identity = 115/319 (36.05%), Postives = 175/319 (54.86%), Query Frame = 0

Query: 13  VETVPETYVFPPEKRPGVAVKMT----IPVIDFGT---HNRALLCQKILDTAQEFGFLQV 72
           V+ VP  YV P   RP ++   T    IP+ID       NRA +  +        GF Q+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREV 132
            NHG+ ++ IK+ M   +EF   S  ++ +  S D  K+ +   S  N + E+V NWR+ 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 133 SDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEMS 192
               CYP++ +++ WP  P  FREV  EY   +R     +LE ISE LG+ K    N + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 GN-PLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQD-VNGLQLFKDGQWIDVDPLDDA 252
            +   +++++YP CP P LT+GL  H D ++IT+L QD V+GLQ+FKDG+WI V+P+ + 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 LLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEA--N 312
            ++N G  +++ISN K K+  HR V N    R S+     P  D +I PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPHYRSIKFKDFIRSFFST 321
           P  YR+  + ++   F+ T
Sbjct: 315 PAIYRNFTYAEYFEKFWDT 332

BLAST of HG10022770 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 106/314 (33.76%), Postives = 170/314 (54.14%), Query Frame = 0

Query: 16  VPETYVFPPEKRPGVAVKM----TIPVIDFGT---HNRALLCQKILDTAQEFGFLQVINH 75
           +P  YV P   RP ++       +IP+ID       NRA++ Q++      +GF Q+ NH
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNH 79

Query: 76  GISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWREVSDF 135
           G+    + +   + +EF      ++ +  S DP K+ +   S  N   ++V NWR+    
Sbjct: 80  GVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKVLNWRDFLRL 139

Query: 136 KCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFENEMSGNP 195
            C+P++ ++  WP  P  FREV  EY   +R    ++LE ISE LG+   +  N +  + 
Sbjct: 140 HCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHA 199

Query: 196 L-LSVSHYPPCPNPSLTFGLIPHCDPDIITILFQD-VNGLQLFKDGQWIDVDPLDDALLI 255
             ++ ++YPPCP P LT+GL  H DP +IT+L QD V+GLQ+FKD +W+ V P+ +  ++
Sbjct: 200 QHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIV 259

Query: 256 NFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIFPENDIIIEPAKSLINEANP-PHY 315
           N G  +++ISN K K+  HR V N    R S+    FP  D +I PA  L+NE +    Y
Sbjct: 260 NIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIY 319

Query: 316 RSIKFKDFIRSFFS 320
           R+  F ++   F++
Sbjct: 320 RTYPFVEYWDKFWN 332

BLAST of HG10022770 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 106/332 (31.93%), Postives = 169/332 (50.90%), Query Frame = 0

Query: 12  GVETVPETYVFPPEKRPGVAVKM-----TIPVIDFGTHN----RALLCQKILDTAQEFGF 71
           GV  VP+ YV PP +RP +   +     T+PVID    +    R+L   +I    +EFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 72  LQVINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNW 131
            QVINHGI   ++ + +    +F  +  E+K    S + ++  ++  S  N++ + VH W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGTSL-NHSTDRVHYW 142

Query: 132 REVSDFKCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVISEGLGIGKGYFEN 191
           R+      +PL K++  WP  PP +++ VG+Y         +++E ISE LG+ K Y + 
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EM-SGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQDVNGLQLFK-DGQWIDVDPL 251
           E+  G+ +++V+ YP CP P +  G+ PH D   +TIL Q   GLQ+   +  W+ V  +
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 252 DDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSL----TYPIFPENDIIIEPAKSL 311
           + AL++  G  +E++SNG  K+  HRV  N    R S     + P+  +    I PA  L
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKK----ISPAPKL 322

Query: 312 INEANPPHYRSIKFKDFIRSFFSTPNIDREIV 329
           +N  N P Y    F DF+    S   I    +
Sbjct: 323 VNPNNAPAYGEFSFNDFLNYISSNDFIQERFI 349

BLAST of HG10022770 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-45
Identity = 115/333 (34.53%), Postives = 173/333 (51.95%), Query Frame = 0

Query: 18  ETYVFPPEKRPGVAVKMT--------IPVIDFGT-----HNRALLCQKILDTAQEFGFLQ 77
           E ++  PE RP   +  +        IP ID  +     H++  + ++I +  + +GF Q
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 78  VINHGISKKLIKETMKIFKEFNAMSTEDKERECSKDPNKSNKFFRSTQNYTNEEVHNWRE 137
           VINHG+   L     K   EF  ++TE+K R+  +D  + N      + +T + V +W+E
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEK-RKVKRD--EVNPMGYHDEEHT-KNVRDWKE 125

Query: 138 VSDF--------------KCYPLDKYVHFWPQKPPKFREVVGEYCVEMRKFASKILEVIS 197
           + DF              +   L K  + WPQ P  FREV  EY  E+ K A ++LE++S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185

Query: 198 EGLGIG----KGYFENEMSGNPLLSVSHYPPCPNPSLTFGLIPHCDPDIITILFQD-VNG 257
             LG+      G+F  + S    L  +HYPPCPNP L  G+  H D   +T+L QD V G
Sbjct: 186 ISLGLPGDRLTGFFNEQTS---FLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGG 245

Query: 258 LQLFK--DGQWIDVDPLDDALLINFGYVLEMISNGKLKATEHRVVTNMMTSRTSLTYPIF 317
           LQ+ +  DGQWI V P+ DAL+IN G  +++ +N +  + EHRVV N    R S+ +  F
Sbjct: 246 LQVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFF 305