HG10022769 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022769 vs. NCBI nr
Match: XP_038899650.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.3e-163
Identity = 279/333 (83.78%), Postives = 306/333 (91.89%), Query Frame = 0

Query: 4   MNFLSNWENVESVPKSYVFPPEKRPCIAVKKTIPVVDFATNDRALLFQKILDVTQEFGIF 63
           MNFLSNWE V+SVPK+YVFPPEKRPC+AVKKTIPV+DFATNDRALLFQ+ILDVTQEFG F
Sbjct: 1   MNFLSNWEGVKSVPKNYVFPPEKRPCVAVKKTIPVIDFATNDRALLFQRILDVTQEFGFF 60

Query: 64  QVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWR 123
           QVINHG+SK+LIEET+KTLKEF+SMSP+DK RECSKDP + CKV+ GS+ SA EQT FWR
Sbjct: 61  QVINHGVSKKLIEETVKTLKEFNSMSPEDKKRECSKDP-DYCKVYPGSQNSANEQTHFWR 120

Query: 124 DSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGE 183
           DS++FNCHPL KYIHFWP+KPP+FREVVG YCVAMRKFVLELLD+ISEG KLG+GYLG E
Sbjct: 121 DSMSFNCHPLEKYIHFWPDKPPEFREVVGEYCVAMRKFVLELLDVISEGLKLGRGYLGDE 180

Query: 184 MSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADPIDDA 243
           MSSNPTM V+YYPPCP P+LTLGLRQHCDGSLITILFQDVNGLQILK+GEWIAADPIDDA
Sbjct: 181 MSSNPTMLVHYYPPCPDPSLTLGLRQHCDGSLITILFQDVNGLQILKDGEWIAADPIDDA 240

Query: 244 LLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNPP 303
           LLINFGYV EVISNGKLKAAEHR +TN  VSR SLIY  CPENDV IEP KCLINETNP 
Sbjct: 241 LLINFGYVLEVISNGKLKAAEHRAMTNMNVSRQSLIYFVCPENDVTIEPAKCLINETNPL 300

Query: 304 RYRSIKFEDFKRKYVSVVANKEEVMKYMSLNKT 337
           RYR+IKFEDF+ KY SV+ANKEE+MKYMSLNKT
Sbjct: 301 RYRTIKFEDFRGKYYSVLANKEEIMKYMSLNKT 332

BLAST of HG10022769 vs. NCBI nr
Match: XP_038899399.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 473.4 bits (1217), Expect = 1.6e-129
Identity = 231/331 (69.79%), Postives = 258/331 (77.95%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRPCIAVKKTIPVVDFATNDRALLFQKILDVTQEF 60
           MAEMNF+SN E VE VP+SYV PPEKRP +AVKKTIPV+DFAT+DRALLFQKILD T EF
Sbjct: 1   MAEMNFVSNLEGVEVVPESYVCPPEKRPDVAVKKTIPVLDFATHDRALLFQKILDSTHEF 60

Query: 61  GIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQ 120
           G FQVINHG+ K+L+EETM    EFHSMSP DK RECSKD N SC+V T S     EQT 
Sbjct: 61  GFFQVINHGVPKKLVEETMSIFMEFHSMSPYDKRRECSKD-NKSCRVFTSSNNYENEQTH 120

Query: 121 FWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYL 180
           FWRD L   CHPL K IHFWP+ PPKFREVV  YCVAM KF  E++D+ISEG  L KGYL
Sbjct: 121 FWRDCLALTCHPLDKNIHFWPQNPPKFREVVRTYCVAMEKFSWEIIDMISEGLGLEKGYL 180

Query: 181 GGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADPI 240
            GEMSSN  +T N+YP CP P+LTLGL QHCD +LITILFQD NGLQ+ K+G WI+ D +
Sbjct: 181 EGEMSSNRRITANHYPICPNPSLTLGLNQHCDRNLITILFQDANGLQVFKDGHWISFDSV 240

Query: 241 DDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINET 300
           DDA LINFGY+FEVISNGKLKA EHRVVTN K SR SL Y   PENDVIIEP KCLINE 
Sbjct: 241 DDAFLINFGYLFEVISNGKLKATEHRVVTNAKASRQSLGYFIFPENDVIIEPAKCLINEA 300

Query: 301 NPPRYRSIKFEDFKRKYVSVVANKEEVMKYM 332
           NP RYRS++ EDF R + +V  N EE MKY+
Sbjct: 301 NPARYRSLELEDFYRHFFAVSTNTEETMKYI 330

BLAST of HG10022769 vs. NCBI nr
Match: XP_004146985.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44748.1 hypothetical protein Csa_016058 [Cucumis sativus])

HSP 1 Score: 464.9 bits (1195), Expect = 5.8e-127
Identity = 209/334 (62.57%), Postives = 268/334 (80.24%), Query Frame = 0

Query: 6   FLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVTQEFGI 65
           F+SNWE VESVP+SYV+PPEKRP    + + K IPV+D +  DR LL +KILD ++EFG 
Sbjct: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67

Query: 66  FQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFW 125
           FQ+INHG+SK++ EETM+  KEFH+MS  +K +ECSKDPN SC+V+T SE   KEQ   W
Sbjct: 68  FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127

Query: 126 RDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGG 185
           RD+L FNCHPL KY+HFWP+KPPK+REVVGAYC+AMRK VLE+L+L+SEG  LGKGY GG
Sbjct: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGG 187

Query: 186 EMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADPIDD 245
           EMS NP + VN+YPPCP P+LTLGL QHCD SLITILFQDVNGLQ+LK+G+WI   PID+
Sbjct: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247

Query: 246 ALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNP 305
           A ++N G+V ++I+NGKL+AAEHR VTN+K SR SL YL  P+++ ++EP KC+INE NP
Sbjct: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307

Query: 306 PRYRSIKFEDFKRKYVSVVANKEEVMKYMSLNKT 337
           PRYRS+ F+DF+R Y+    + + VM+Y+  N++
Sbjct: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of HG10022769 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 463.4 bits (1191), Expect = 1.7e-126
Identity = 211/339 (62.24%), Postives = 270/339 (79.65%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVT 60
           M E+ F+SNWE V+SVP  YV+PPEKRP    + +  TIPV+D +T DR LL +KILD +
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKE 120
           QEFG FQVINHG+SK+++EET +  K+FH+MS  +K +ECS+DPN SC+V+T SE   KE
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTQFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGK 180
           QT  WRD+L FNCHPL KY+HFWP+ PPK+REVVGAYCV MR  VLE+L+L+SEG  LG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAA 240
           GYLGGEMS NP M VN+YPPCP P+LTLGL QHCD SLITILFQ+VNGLQ+LK+G+WI  
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 DPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLI 300
            PID+A ++N G+V +VISNGKLKAAEHR VTN K+SR SL YL  P+++V +EP KCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSIKFEDFKRKYVSVVANKEEVMKYMSLNKT 337
           NE NPPRYR++KF+DF+R Y+   ++ + VM+ +  +++
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339

BLAST of HG10022769 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 463.0 bits (1190), Expect = 2.2e-126
Identity = 210/332 (63.25%), Postives = 267/332 (80.42%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVT 60
           M E+ F+SNWE V+SVP  YV+PPEKRP    + +   IPV+D +T DR LL +KILD +
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKE 120
           QEFG FQVINHG+S++++EET +  K+FH+MS  +K +ECS+DPN SC+V+T SE   KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTQFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGK 180
           QT  WRD+L FNCHPL KY+HFWP+ PPK+REVVGAYC+AMR  VLE+L+L+SEG  LG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAA 240
           GYLGGEMS NP M VN+YPPCP P+LTLGL QHCD SLITILFQ+VNGLQ+LK+G+WI  
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 DPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLI 300
            PID+A ++N G+V +VISNGKLKAAEHRVVTN K+SR SL YL  P+++V +EP KCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSIKFEDFKRKYVSVVANKEEVMK 330
           NE NPPRYR++KF+DF+R Y+   ++ + VM+
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVME 332

BLAST of HG10022769 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.4e-62
Identity = 133/342 (38.89%), Postives = 200/342 (58.48%), Query Frame = 0

Query: 6   FLSNWENVESVPKSYVFPPEKR--PCIAVKKTIPVVDFATNDRALLFQKILDVTQEFGIF 65
           F+SNW + +SV +S++ P +KR    + V   +P++D   +   LL Q+I    Q+FG+F
Sbjct: 4   FVSNW-STKSVSESFIAPLQKRAEKDVPVGNDVPIIDLQQHHH-LLVQQITKACQDFGLF 63

Query: 66  QVINHGLSKQLIEETMKTLKEFHSMSPQDKNR-----ECSK---DPNNSCKVHTGSETSA 125
           QVINHG  ++L+ ETM+  KEF ++  ++K +     E +K         K++   E  +
Sbjct: 64  QVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLS 123

Query: 126 KEQTQFWRDSLTFNCHPLHK-YIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPK 185
            E+  +W+D+L   CHPL +  ++ WPEKP K+REVV  Y V +RK  + +LD I EG  
Sbjct: 124 NEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGLG 183

Query: 186 LGKGYLGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQ--ILKNG 245
           L  GY   E+S    M  NYYPPCP P+ TLG   H DG+LIT+L QD+ GLQ  I+K+ 
Sbjct: 184 LKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKDA 243

Query: 246 EWIAADPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEP 305
            WIA  PI  A ++N G   +VI+N K + + HRVVT+    R+S+  L  P+    IEP
Sbjct: 244 TWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIEP 303

Query: 306 TKCLINETNPPRYRSIKFEDFKRKYVSVVANKEEVMKYMSLN 335
            K L+N+ NPP Y+   + +F   Y+S  ++ +  +K   +N
Sbjct: 304 AKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDSGVKPYKIN 343

BLAST of HG10022769 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-59
Identity = 125/326 (38.34%), Postives = 191/326 (58.59%), Query Frame = 0

Query: 15  SVPKSYVFPPEKRPC---IAVKKTIPVVDFATNDRALLFQKILDVTQEFGIFQVINHGLS 74
           ++P++YV P   RP    ++  +  P++D ++ DR+ L Q+I      FG FQVINHG++
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDSLTFNCH 134
           KQ+I+E +   +EF SMS ++K +  S DP  + ++ T S    KE+   WRD L  +C+
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLST-SFNVKKEEVNNWRDYLRLHCY 133

Query: 135 PLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLG---GEMSSNP 194
           P+HKY++ WP  PP F+E+V  Y   +R+   ++ +LISE   L K Y+    GE   + 
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQH- 193

Query: 195 TMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD--VNGLQILKNGEWIAADPIDDALLI 254
            M VNYYPPCP P LT GL  H D + +TIL QD  V GLQIL +G+W A +P  DA +I
Sbjct: 194 -MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVI 253

Query: 255 NFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCL---INETNPP 314
           N G   + +SNG  K+  HR VTNT+  RLS+    CP +  ++ P K L    ++   P
Sbjct: 254 NIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKP 313

Query: 315 RYRSIKFEDFKRKYVSVVANKEEVMK 330
            Y+   + ++ +K+ S   ++E  ++
Sbjct: 314 VYKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of HG10022769 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.0e-57
Identity = 119/322 (36.96%), Postives = 179/322 (55.59%), Query Frame = 0

Query: 14  ESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVTQEFGIFQVINHGL 73
           +++P  YV P  +RP    +     IPVVD A+ DRA +   + D  +  G FQV+NHG+
Sbjct: 18  DTMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGI 77

Query: 74  SKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDSLTFNC 133
              LI   M+  +EF  +  ++K +  S DP    ++ T S    KE    WRD L  +C
Sbjct: 78  DAALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLST-SFNVRKETVHNWRDYLRLHC 137

Query: 134 HPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGEMSSNPT- 193
           +PLH+++  WP  PP F+E++G YC  +R+    L + ISE   L  GY+   +      
Sbjct: 138 YPLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 197

Query: 194 MTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD--VNGLQILKNGEWIAADPIDDALLIN 253
           M VNYYP CP P LT GL  H D + +TIL  D  V GLQ+L +G+WIA +P   AL+IN
Sbjct: 198 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 257

Query: 254 FGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNPPRYRS 313
            G   + +SNGK ++  HR V N+   R+S+    CP N V + P K LI + +P  YR+
Sbjct: 258 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 317

Query: 314 IKFEDFKRKYVSVVANKEEVMK 330
             ++++ +K+ S   ++E  ++
Sbjct: 318 YTYDEYYKKFWSRNLDQEHCLE 338

BLAST of HG10022769 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 113/316 (35.76%), Postives = 174/316 (55.06%), Query Frame = 0

Query: 13  VESVPKSYVFPPEKRPCIAVKKT----IPVVDFAT---NDRALLFQKILDVTQEFGIFQV 72
           V+ VP +YV P   RP ++  +T    IP++D       +RA +  +        G FQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDS 132
            NHG+ ++ I++ M   +EF   S  ++ +  S D   + ++ T    S KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVS-KEKVSNWRDF 134

Query: 133 LTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGEMS 192
           L  +C+P+  +I+ WP  P  FREV   Y  ++R  VL LL+ ISE   L K  +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 SN-PTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD-VNGLQILKNGEWIAADPIDDA 252
            +   M +NYYP CP P LT GL  H D +LIT+L QD V+GLQ+ K+G+WIA +P+ + 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 LLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLIN--ETN 312
            ++N G   +VISN K K+  HR V N+ + R+S+    CP  D +I P + LIN  E +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPRYRSIKFEDFKRKY 318
           P  YR+  + ++  K+
Sbjct: 315 PAIYRNFTYAEYFEKF 329

BLAST of HG10022769 vs. ExPASy Swiss-Prot
Match: Q40061 (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.7e-50
Identity = 116/326 (35.58%), Postives = 178/326 (54.60%), Query Frame = 0

Query: 12  NVESVPKSYVFPPEKRPCIAVKKTIPVVDFATNDRALLFQKILDVTQEFGIFQVINHGLS 71
           +  S+P S++ P ++        ++P++D  +  R  + Q ILD  +E+G  QV+NHG+S
Sbjct: 11  HASSIPDSFLLPADRLHPATTDVSLPIIDM-SRGRDEVRQAILDSGKEYGFIQVVNHGIS 70

Query: 72  KQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDSLTFNCH 131
           + ++ E      EF  M  +DK    S+D +   K+  GS      + ++W D L    +
Sbjct: 71  EPMLHEMYAVCHEFFDMPAEDKAEFFSEDRSERNKLFCGSAFETLGE-KYWIDVLEL-LY 130

Query: 132 PLHK-YIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGEMSSNPTM 191
           PL       WP KP   REVVG Y    R   +E+L L+ EG  L   +  G++S    +
Sbjct: 131 PLPSGDTKDWPHKPQMLREVVGNYTSLARGVAMEILRLLCEGLGLRPDFFVGDISGGRVV 190

Query: 192 T-VNYYPPCPYPTLTLGLRQHCDGSLITILFQD-VNGLQILKNGEWIAADPIDDALLINF 251
             +NYYPP P P+ TLGL  HCD  L+T+L    V GL+I   G WI   P+ ++L+INF
Sbjct: 191 VDINYYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWIKVQPVPNSLVINF 250

Query: 252 GYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNPPRYRSI 311
           G   EV++NG LKA EHR  TN    RLS+     P +D ++ P +  ++E NPPRYR++
Sbjct: 251 GLQLEVVTNGYLKAVEHRAATNFAEPRLSVASFIVPADDCVVGPAEEFVSEDNPPRYRTL 310

Query: 312 KFEDFKRKYVSVVANKEEVMKYMSLN 335
              +FKRK+ +VV     + + +++N
Sbjct: 311 TVGEFKRKH-NVVNLDSSINQIININ 332

BLAST of HG10022769 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.8e-127
Identity = 209/334 (62.57%), Postives = 268/334 (80.24%), Query Frame = 0

Query: 6   FLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVTQEFGI 65
           F+SNWE VESVP+SYV+PPEKRP    + + K IPV+D +  DR LL +KILD ++EFG 
Sbjct: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67

Query: 66  FQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFW 125
           FQ+INHG+SK++ EETM+  KEFH+MS  +K +ECSKDPN SC+V+T SE   KEQ   W
Sbjct: 68  FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHCW 127

Query: 126 RDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGG 185
           RD+L FNCHPL KY+HFWP+KPPK+REVVGAYC+AMRK VLE+L+L+SEG  LGKGY GG
Sbjct: 128 RDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGG 187

Query: 186 EMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADPIDD 245
           EMS NP + VN+YPPCP P+LTLGL QHCD SLITILFQDVNGLQ+LK+G+WI   PID+
Sbjct: 188 EMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDN 247

Query: 246 ALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNP 305
           A ++N G+V ++I+NGKL+AAEHR VTN+K SR SL YL  P+++ ++EP KC+INE NP
Sbjct: 248 AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANP 307

Query: 306 PRYRSIKFEDFKRKYVSVVANKEEVMKYMSLNKT 337
           PRYRS+ F+DF+R Y+    + + VM+Y+  N++
Sbjct: 308 PRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of HG10022769 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 6.9e-126
Identity = 209/332 (62.95%), Postives = 267/332 (80.42%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVT 60
           M E+ F+SNWE V+SVP  YV+PPEKRP    + +   IPV+D +T+DR LL +KILD +
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKE 120
           QEFG FQVINHG+S++++EET +  K+FH+MS  +K +ECS+DPN SC+V+T SE   KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTQFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGK 180
           QT  WRD+L FNCHPL KY+HFWP+ PPK+REVVGAYC+AMR  VLE+L+L+SEG  LG+
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAA 240
           GYLGGEMS NP M VN+YP CP P+LTLGL QHCD SLITILFQ+VNGLQ+LK+G+WI  
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 DPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLI 300
            PID+A ++N G+V +VISNGKLKAAEHRVVTN K+SR SL YL  P+++V +EP KCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSIKFEDFKRKYVSVVANKEEVMK 330
           NE NPPRYR++KF+DF+R Y+   ++ + VM+
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVME 332

BLAST of HG10022769 vs. ExPASy TrEMBL
Match: A0A0A0K4U9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 5.8e-125
Identity = 220/332 (66.27%), Postives = 258/332 (77.71%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRPCIAVKKTIPVVDFATNDRALLFQKILDVTQEF 60
           MA+MNF+SNWE VESVP+SYV+P EKRP IA+KKTIPV+DFAT DRALLFQKILD T EF
Sbjct: 1   MAQMNFVSNWEGVESVPESYVWPQEKRPGIALKKTIPVLDFATQDRALLFQKILDSTHEF 60

Query: 61  GIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQ 120
           G+FQVINHG+SK+L+EETM   KEFHSM PQ K RE SKD N SCKV T S     EQT 
Sbjct: 61  GVFQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTH 120

Query: 121 FWRDSLTFNCHP-LHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGY 180
            WRD L+  C+P LH+ IH WP+ PPK REVVGAYC+AM KF  E++DLISEG  LG+GY
Sbjct: 121 LWRDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGY 180

Query: 181 LGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADP 240
             GEMSS   +T N+YP CP P+LT+GL QHCD  L+TILFQDV+GLQ+ K+G WI+ DP
Sbjct: 181 FEGEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDP 240

Query: 241 IDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINE 300
           IDDA ++NFGY+ EVISNGKLKA EHRVVTN + SR S  Y+  PE ++IIEP KCLINE
Sbjct: 241 IDDAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINE 300

Query: 301 TNPPRYRSIKFEDFKRKYVSVVANKEEVMKYM 332
            NPP YRS K EDF R + +V  N EE MK++
Sbjct: 301 ANPPHYRSFKVEDFNRHFFAVSNNNEETMKFI 331

BLAST of HG10022769 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 1.0e-124
Identity = 208/337 (61.72%), Postives = 265/337 (78.64%), Query Frame = 0

Query: 1   MAEMNFLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVT 60
           M E+ F+SNWE V+SVP  Y++PPEKRP    + +   IPVVD +T DR LL +KILD +
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKE 120
           QEFG FQVINHG+S++++EET +  K+FH+MS  +K +ECS+DP  SC+V+T SE   KE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTQFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGK 180
           QT  WRD+L FNCHPL KY+HFWP+ PPK+REVVGAYCVAMR  VLE+L+L+SEG  LG+
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAA 240
           GYL  EMS NP M VN+YPPCP P+LTLGL QHCD SLITILFQ+VNGLQ+LK+G+WI  
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 DPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLI 300
            PID+A ++N G+V +VISNGKLKAAEHR VTN K+SR SL YL  P+++V +EP KCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NETNPPRYRSIKFEDFKRKYVSVVANKEEVMKYMSLN 335
           NE NPPRYR++KF+DF+R Y+   ++ + VM+ +  N
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSN 337

BLAST of HG10022769 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.8e-124
Identity = 207/337 (61.42%), Postives = 264/337 (78.34%), Query Frame = 0

Query: 3   EMNFLSNWENVESVPKSYVFPPEKRP---CIAVKKTIPVVDFATNDRALLFQKILDVTQE 62
           +  F+SNWE  ESVP+SYV+PPEKRP    + + K IPV+D +  DRALL +KIL  ++E
Sbjct: 5   DAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASKE 64

Query: 63  FGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQT 122
            G FQ+INHG+SK++ E TM+  KEFH+MS  +K +ECSKDPN SC+V+T SE   KEQ 
Sbjct: 65  SGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQI 124

Query: 123 QFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGY 182
             WRD+L FNCHPL KY+HFWP+ PPK+REVVGAYCVAMRK VLE+L+L+SEG  LGKGY
Sbjct: 125 HCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKGY 184

Query: 183 LGGEMSSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILKNGEWIAADP 242
           LGGEMS NP + VN+YPPCP P+LTLGL QHCD SLITILFQDVNGLQ+LK+G+WI   P
Sbjct: 185 LGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQP 244

Query: 243 IDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINE 302
           ID A ++N G+V ++I+NGKL+AAEHR VTN+K SR SL YL  P+++  +EP KC+INE
Sbjct: 245 IDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMINE 304

Query: 303 TNPPRYRSIKFEDFKRKYVSVVANKEEVMKYMSLNKT 337
            NPPRYRS+ F+DF+R Y+    + + VM+Y+  N++
Sbjct: 305 ANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of HG10022769 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.7e-60
Identity = 125/326 (38.34%), Postives = 191/326 (58.59%), Query Frame = 0

Query: 15  SVPKSYVFPPEKRPC---IAVKKTIPVVDFATNDRALLFQKILDVTQEFGIFQVINHGLS 74
           ++P++YV P   RP    ++  +  P++D ++ DR+ L Q+I      FG FQVINHG++
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 75  KQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDSLTFNCH 134
           KQ+I+E +   +EF SMS ++K +  S DP  + ++ T S    KE+   WRD L  +C+
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLST-SFNVKKEEVNNWRDYLRLHCY 133

Query: 135 PLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLG---GEMSSNP 194
           P+HKY++ WP  PP F+E+V  Y   +R+   ++ +LISE   L K Y+    GE   + 
Sbjct: 134 PIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQH- 193

Query: 195 TMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD--VNGLQILKNGEWIAADPIDDALLI 254
            M VNYYPPCP P LT GL  H D + +TIL QD  V GLQIL +G+W A +P  DA +I
Sbjct: 194 -MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVI 253

Query: 255 NFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCL---INETNPP 314
           N G   + +SNG  K+  HR VTNT+  RLS+    CP +  ++ P K L    ++   P
Sbjct: 254 NIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKP 313

Query: 315 RYRSIKFEDFKRKYVSVVANKEEVMK 330
            Y+   + ++ +K+ S   ++E  ++
Sbjct: 314 VYKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of HG10022769 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 113/316 (35.76%), Postives = 174/316 (55.06%), Query Frame = 0

Query: 13  VESVPKSYVFPPEKRPCIAVKKT----IPVVDFAT---NDRALLFQKILDVTQEFGIFQV 72
           V+ VP +YV P   RP ++  +T    IP++D       +RA +  +        G FQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDS 132
            NHG+ ++ I++ M   +EF   S  ++ +  S D   + ++ T    S KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVS-KEKVSNWRDF 134

Query: 133 LTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGEMS 192
           L  +C+P+  +I+ WP  P  FREV   Y  ++R  VL LL+ ISE   L K  +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 SN-PTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD-VNGLQILKNGEWIAADPIDDA 252
            +   M +NYYP CP P LT GL  H D +LIT+L QD V+GLQ+ K+G+WIA +P+ + 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 LLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLIN--ETN 312
            ++N G   +VISN K K+  HR V N+ + R+S+    CP  D +I P + LIN  E +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPRYRSIKFEDFKRKY 318
           P  YR+  + ++  K+
Sbjct: 315 PAIYRNFTYAEYFEKF 329

BLAST of HG10022769 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 106/321 (33.02%), Postives = 169/321 (52.65%), Query Frame = 0

Query: 7   LSNWENVESVPKSYVFPPEKRPCIAVKK----TIPVVDFAT---NDRALLFQKILDVTQE 66
           +S++ +   +P +YV P   RP ++  +    +IP++D       +RA++ Q++      
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGIFQVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQT 126
           +G FQ+ NHG+    + +     +EF      ++ +  S DP  + ++ T     A ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGA-DKV 130

Query: 127 QFWRDSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGY 186
             WRD L  +C P+  +I  WP  P  FREV   Y  ++R  VL LL+ ISE   L   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 LGGEMSSNPT-MTVNYYPPCPYPTLTLGLRQHCDGSLITILFQD-VNGLQILKNGEWIAA 246
           +   +  +   M  NYYPPCP P LT GL  H D ++IT+L QD V+GLQ+ K+ +W+A 
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 DPIDDALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLI 306
            PI +  ++N G   +VISN K K+  HR V NT+  RLS+     P  D +I P   L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NETNP-PRYRSIKFEDFKRKY 318
           NE +    YR+  F ++  K+
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKF 330

BLAST of HG10022769 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 184.5 bits (467), Expect = 1.4e-46
Identity = 102/306 (33.33%), Postives = 157/306 (51.31%), Query Frame = 0

Query: 16  VPKSYVFPPEKRPCIAVKKTIPVVDFATNDRALLFQKILDVTQEFGIFQVINHGLSKQLI 75
           VP  YV PP  +PC +    +P +D +         ++     E     ++NHG+++ ++
Sbjct: 24  VPDCYVVPPSSKPCDSNSGIVPTIDVS---------RLKGGDDERR--GIVNHGINQNIL 83

Query: 76  EETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWRDSLTFNCHPLHK 135
           ++ ++    F  +  ++K +  S D     +  T S     +  QFWR  L    HPLH+
Sbjct: 84  DDALEVANSFFELPAKEKKQFMSNDVYAPVRYST-SLKDGLDTIQFWRIFLKHYAHPLHR 143

Query: 136 YIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGEMSSN--PTMTVN 195
           +IH WPE PP +RE +G +C  +RK  +EL+  I+E   LG+ YL   M  N    MTVN
Sbjct: 144 WIHLWPENPPGYREKMGKFCEEVRKLSIELMGAITESLGLGRDYLSSRMDENGMQVMTVN 203

Query: 196 YYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILK------NGEWIAADPIDDALLIN 255
            YPPCP P   LGL  H D S IT+L Q+++GL+I        +G W+    +   L ++
Sbjct: 204 CYPPCPDPETALGLPPHSDYSCITLLLQNLDGLKIFDPMAHGGSGRWVGVPQVTGVLKVH 263

Query: 256 FGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETNPPRYRS 314
            G   EV+SNG  K+  H+V  N + +R+SL  L     D  +   + L+N+ NP RY+ 
Sbjct: 264 IGDHVEVLSNGLYKSIVHKVTLNEEKTRISLASLHSLGMDDKMSVPRELVNDENPVRYKE 317

BLAST of HG10022769 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-46
Identity = 108/312 (34.62%), Postives = 155/312 (49.68%), Query Frame = 0

Query: 13  VESVPKSYVFPPEKRPCI-----AVKKTIPVVDFATND----RALLFQKILDVTQEFGIF 72
           V  VP  YV PP +RP +       + T+PV+D +       R+L   +I    +EFG F
Sbjct: 24  VPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFF 83

Query: 73  QVINHGLSKQLIEETMKTLKEFHSMSPQDKNRECSKDPNNSCKVHTGSETSAKEQTQFWR 132
           QVINHG+   ++ + +    +F  +  ++K    S + +   +  T S   + ++  +WR
Sbjct: 84  QVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT-SLNHSTDRVHYWR 143

Query: 133 DSLTFNCHPLHKYIHFWPEKPPKFREVVGAYCVAMRKFVLELLDLISEGPKLGKGYLGGE 192
           D +    HPL K+I  WP  PP +++ VG Y  A      +L++ ISE   L K YL  E
Sbjct: 144 DFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQEE 203

Query: 193 M-SSNPTMTVNYYPPCPYPTLTLGLRQHCDGSLITILFQDVNGLQILK-NGEWIAADPID 252
           +   +  M VN YP CP P + LG+  H D S +TIL Q   GLQI+  N  W+    I+
Sbjct: 204 IEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYIE 263

Query: 253 DALLINFGYVFEVISNGKLKAAEHRVVTNTKVSRLSLIYLACPENDVIIEPTKCLINETN 312
            AL++  G   EV+SNG  K+  HRV  N +V RLS   L        I P   L+N  N
Sbjct: 264 GALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPNN 323

Query: 313 PPRYRSIKFEDF 314
            P Y    F DF
Sbjct: 324 APAYGEFSFNDF 334