HG10022666 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022666
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	membrane protein of ER body-like protein
Location	Chr05: 26784823 .. 26788359 (+)
RNA-Seq Expression	HG10022666
Synteny	HG10022666

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTCACTAAAGTTTATTGTCAGTGTGACAATGGTAGTCGCCGACAATGGCTGATGGTTGAGCCATTGAAAACACTGGAAGAAGAGAGAGTTGAGGTGAGTTGGTAATTTATACTAACTCAACTCCACCAATATTTAAAATTGGTGAGCGAAACATTGAGTTGGTATATTTTAAACTCAACTCAATTCATGTTGGTGAGCTAAATACACCTCCTTAATCTTCCCCCATCTTCAATTTGAATATTTGCTCATTAATTCATGATGTTTTTTTTTTCTATGATTTCCATAACCTTTGATGATTTACATGTATTACTTTATTATAAGATGGTTGTTATTCATTTTTTTTAAGTCAACACAAATGCATCGATACTAATTCAAGTCAACACAAAGTCATAATTGATACAGTACTACCATATGAAATTGGCTCTTCACATGCTGTTCAACAAGCAGAAGCCATTGATACAGAAATCGAAGTATTATCGCACGAAATTGGTTAGTTGTTTTTCTTCTGTTGATTTTTTTCAATACAATAGAAGTTGGAGGATTCGAATCACATACTATTTTTTTAATTACTAATACATCTATATTTCAATTGAATTATAATCACTTTCACAAAATTGTTTTGTCACGGTAGTTTTTTTCTAATTTATATAGTGATAATGATCAACTCTTTTTTAAATTAAAAAAAAAAAAAAAAAACTTTTTGCATCTTCCTCTCTTTTCTATTCTCTCTATTTGTTTAAGTGTTATAAACACACCTATATCATAATTTGTTTTTTTTTTTTTCCTTATTTCGTTGATTTCCACGACTTTTCATGATTTATATGTATTTTTATTTATAAGAGGGTTGTTTTTTTAATTTTAAACTGAGATGTTTTCAAATATAGAAAAATGAGTCAAACAATTTATAAATATAGAAAAATTCTACTGTCATCTATTAGACCGTCATAGACTTCTATCATTTAAGCGATAGGCCGCAATGGACTTCTATATTTATAAAGAGTTTAATATTTTTTTTGTTTATAATAATGTTTCTTTTAAATTTTACACTTTTTTTTTTTTTTGAACTTAAACCTTTCATAAGAGACCAGGAAAGCATGTGGTAAAAGTGCAAATATGGACAAACGACAAAGAAATAGAGAAAGAAAGTGGGAAATTCAGAAATGTATAGTAGTTGGTGGTTTATTCGAATCAATAACAAGTTTATTCATCGTAACCTCTGCAGCAAGTGTCCAAAATTTGGATGGTAACCCTTTTCAATTTCTTTATTCCCTTCTCTACTAAAAAATTATGTGATTTCAATTTTCAATTCCTAAATCAATTTGATTTAGGGTTGTTTTCAATATAGAAAAATGTATCAAACTATTTACAAATATAGAATAATTTTACTGTTTATTGAATTCTATCGCTCAAACGATAGAAATCTATCACGGACTATTGTTGATAGACAGTAAAATTTTTCTATATTTATAAATAGTTTGATATTTTTTCTGTTTACAGTAATTTCCTTTTGATTTACTTCTTTTCATTCATACTTGTATTATATATATACAACTGCAGGGAATATTATAGGACTGGCATTGACAAACTTGTTTACTGGACTCTTTCTCATTAGACATGATGTAAGTAATAATTACTTGTGCTATCAAATACAACCTAAATAATATTTAGCACAAAATTTTAAGGCTATACTTAATTAGTTTACACATATGTTTAAACTTTTAATATTTATAAATACTGTTTTGGATTTTTGTATTTCTTTTAGTTTTATTTTTTATTTTGTCTTTAAAATTTAAGAATTATTGTCCATTTTTATCTCTAAACTTTCAGACTTTTATTTATTTTAGTGCATATACTTTCAAAATGTTCCCTTTTATTTCCGTGCATTAAAAATATAGCTCTTCTTCAATGGTTGCAAAATTAATATAACAAAAATTCTGTACATAATAAAACAAAAAACATATTTTAACTCAAAGTTATGGTTATATTTCAAGAAATCTATCGAGTGTTTGTTAGAAGAGAAAAATGCATGAATGACACAAATGTAGAGAAAGTGATAATTCCATTCAACTAAACATACCTTTATATAGGCTTACAAAAGATAATCATAGAATCTCAATGATTAAAAAACTATAAATATTTCTAAATGCTGGTAATTTCTAACACAAATAAAACTCCCAAATTAGAACTCAAAAGCCACGCTAAGATTACAAAACACCAAAAAATCACCAATTAAGTTTAATGCTAAAATCTTTTATTTTCTCATTATTAATGAAACCTTTCATATTCTCCAACAGTGTCTAGCTAGTTTTTCCTTGCCAAATTGTGTGACTGAAAAAATGAGATAAAAGAAAACAGATATTTATTGATTACAACTTATAATTCTTAGTCTAGATCTCAAATCTCGATGTAAAGTACATCTTAATCTAAAGAAATCAAAATAATATATGGTTTTATTTATGCTCTCTCATTCTTTATTTATAGAAAAGGAATTTTTAAAAATAAAAAAATAAGAAAAATTATTTACACAAAATAACAAAATTTTTAGATAATTGTAATAGATGCTGATAGAAGTCTGTGAAGGTCTATCAGTGATAGAAATTATAGAAGTCTGTCACTAATAGATGCTGATAGAAGTTTATCAATGTCTATCAGTGTTTTATTTTATTTTATTTTATTTGTTATTTTCTGTAAATAGTGTGACATTTTTTCAATTTGTGAAAATTTCCCTTTTGGTGGAAAACATTATTTGATATTGGGAGTATGCTAGGGAGTAGTTTTGAAAGGGATTTTTAAAAGAGCAAGGAAGGATACACCTTAACCACCCATGCTCCTCTCTTCTCTACCACACAAATAAAAGAATAATTTTAAAAAATATATACTTTTAAAAAAGAAAAGTATCACGTTGTGCGTTCCAATTGGATGGCAGCCTGCACAAAAGATGGGTGCAACTAAATTTTTCCACTTTTAAAATAAGAAATGGATACTAGAAATAGTTTGTTTAGCTAACATGTAAAATTGTACTAAACACTCATAATTATTTGATAAATACTTTGAATACAACCCATGCAAAACCTATACATGAACAAAAACATTAATTTCTTCAAATTGCTGAAATTTAATTTAAATACTTTACAGTTACCAAGACTCAAGAAGAATAATCCTAATAAGGGAACTGAAGTGGGAAGATGGAGTCACTACAAAGAAGAAGTGAAAGATAAAATGCATAGTCTTCTTCATTTTGCGATTGCTTTCTTATCTTTCATATTTTTTGGTTTAGTGCCTCCATTTGTTTATGCATTATTCTCATTCCACAACACTGGTAACAAGGAGCTCAAGCTTGTAGCTACTGCAGGAGCTTCCCTTTTATGCACAGCATTTCTTGCTATTCAGAAAGCTCACATTGGAAGACCAGAAAATAGGGTTATCTATATCAAAACTGCAGCCATTTATGTTTCAATTGGAATTGGGGCTTTTGTGTTATCATATTTGGCAGGTTACATGTTTGGTCAACTCACAGAGAAGCTTCCTTGA

mRNA sequence

ATGATTTTCACTAAAGTTTATTGTCAGTGTGACAATGGTAGTCGCCGACAATGGCTGATGGTTGAGCCATTGAAAACACTGGAAGAAGAGAGAGTTGAGTTACCAAGACTCAAGAAGAATAATCCTAATAAGGGAACTGAAGTGGGAAGATGGAGTCACTACAAAGAAGAAGTGAAAGATAAAATGCATAGTCTTCTTCATTTTGCGATTGCTTTCTTATCTTTCATATTTTTTGGTTTAGTGCCTCCATTTGTTTATGCATTATTCTCATTCCACAACACTGGTAACAAGGAGCTCAAGCTTGTAGCTACTGCAGGAGCTTCCCTTTTATGCACAGCATTTCTTGCTATTCAGAAAGCTCACATTGGAAGACCAGAAAATAGGGTTATCTATATCAAAACTGCAGCCATTTATGTTTCAATTGGAATTGGGGCTTTTGTGTTATCATATTTGGCAGGTTACATGTTTGGTCAACTCACAGAGAAGCTTCCTTGA

Coding sequence (CDS)

ATGATTTTCACTAAAGTTTATTGTCAGTGTGACAATGGTAGTCGCCGACAATGGCTGATGGTTGAGCCATTGAAAACACTGGAAGAAGAGAGAGTTGAGTTACCAAGACTCAAGAAGAATAATCCTAATAAGGGAACTGAAGTGGGAAGATGGAGTCACTACAAAGAAGAAGTGAAAGATAAAATGCATAGTCTTCTTCATTTTGCGATTGCTTTCTTATCTTTCATATTTTTTGGTTTAGTGCCTCCATTTGTTTATGCATTATTCTCATTCCACAACACTGGTAACAAGGAGCTCAAGCTTGTAGCTACTGCAGGAGCTTCCCTTTTATGCACAGCATTTCTTGCTATTCAGAAAGCTCACATTGGAAGACCAGAAAATAGGGTTATCTATATCAAAACTGCAGCCATTTATGTTTCAATTGGAATTGGGGCTTTTGTGTTATCATATTTGGCAGGTTACATGTTTGGTCAACTCACAGAGAAGCTTCCTTGA

Protein sequence

MIFTKVYCQCDNGSRRQWLMVEPLKTLEEERVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAIYVSIGIGAFVLSYLAGYMFGQLTEKLP

Homology

[Close]

BLAST of HG10022666 vs. NCBI nr
Match: XP_023548918.1 (uncharacterized protein LOC111807425 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K KM+SLL F IA+LSF+FFGLVPP VYAL S
Sbjct: 806 RHDVSRLQKKRKIPRIEVD-GNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSS 865

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P NR++Y+KTA +YVS+G+GAF +S
Sbjct: 866 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGIS 925

Query: 151 YLAGYMFGQLTEKL 164
           YLAGY+FG L E+L
Sbjct: 926 YLAGYIFGHLAEEL 937

[Close]

BLAST of HG10022666 vs. NCBI nr
Match: XP_023548919.1 (uncharacterized protein LOC111807425 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K KM+SLL F IA+LSF+FFGLVPP VYAL S
Sbjct: 805 RHDVSRLQKKRKIPRIEVD-GNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSS 864

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P NR++Y+KTA +YVS+G+GAF +S
Sbjct: 865 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGIS 924

Query: 151 YLAGYMFGQLTEKL 164
           YLAGY+FG L E+L
Sbjct: 925 YLAGYIFGHLAEEL 936

[Close]

BLAST of HG10022666 vs. NCBI nr
Match: XP_023548916.1 (uncharacterized protein LOC111807425 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023548917.1 uncharacterized protein LOC111807425 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K KM+SLL F IA+LSF+FFGLVPP VYAL S
Sbjct: 811 RHDVSRLQKKRKIPRIEVD-GNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSS 870

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P NR++Y+KTA +YVS+G+GAF +S
Sbjct: 871 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGIS 930

Query: 151 YLAGYMFGQLTEKL 164
           YLAGY+FG L E+L
Sbjct: 931 YLAGYIFGHLAEEL 942

[Close]

BLAST of HG10022666 vs. NCBI nr
Match: XP_023548920.1 (uncharacterized protein LOC111807425 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K KM+SLL F IA+LSF+FFGLVPP VYAL S
Sbjct: 773 RHDVSRLQKKRKIPRIEVD-GNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSS 832

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P NR++Y+KTA +YVS+G+GAF +S
Sbjct: 833 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGIS 892

Query: 151 YLAGYMFGQLTEKL 164
           YLAGY+FG L E+L
Sbjct: 893 YLAGYIFGHLAEEL 904

[Close]

BLAST of HG10022666 vs. NCBI nr
Match: XP_023548921.1 (uncharacterized protein LOC111807425 isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-30
Identity = 78/134 (58.21%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K KM+SLL F IA+LSF+FFGLVPP VYAL S
Sbjct: 767 RHDVSRLQKKRKIPRIEVD-GNYYKEILKGKMYSLLCFIIAYLSFLFFGLVPPLVYALSS 826

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P NR++Y+KTA +YVS+G+GAF +S
Sbjct: 827 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAGVYVSVGVGAFGIS 886

Query: 151 YLAGYMFGQLTEKL 164
           YLAGY+FG L E+L
Sbjct: 887 YLAGYIFGHLAEEL 898

[Close]

BLAST of HG10022666 vs. ExPASy Swiss-Prot
Match: F4KFS7 (Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-14
Identity = 45/114 (39.47%), Postives = 68/114 (59.65%), Query Frame = 0


Query: 54  YKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTA 113
           Y+E +  +  S +H  +A +S+IFFGL+PP VYA FSF+ TG K  KL++    SL+C  
Sbjct: 411 YEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYA-FSFYETGIKNYKLISVFLGSLVCVI 470

Query: 114 FLAIQKAHIGRPENRV----IYIKTAAIYVSIGIGAFVLSYLAGYMFGQLTEKL 164
            L   K ++ +P N       Y+K+AA Y SI + +  +SY+ G + G+  EKL
Sbjct: 471 LLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKL 523

[Close]

BLAST of HG10022666 vs. ExPASy Swiss-Prot
Match: Q8LPT3 (Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.8e-13
Identity = 55/145 (37.93%), Postives = 75/145 (51.72%), Query Frame = 0


Query: 19  LMVEPLKTLEEERVELPRLKKNNPN-KGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIF 78
           L++  L+ L EE       + N  N +  E GR   YK  +  + +  LH  +A LSFI 
Sbjct: 591 LIIHNLQELREEEPIRTTTEDNQTNGREEEEGR---YKRLLGRRENFTLHATVAILSFII 650

Query: 79  FGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAI 138
            G++PP VY  FSF    NK+ K+ +  GASL C   LAI KAH+  P  R  Y+K+   
Sbjct: 651 TGILPPVVY-YFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYP--RGSYLKSILY 710

Query: 139 YVSIGIGAFVLSYLAGYMFGQLTEK 163
           Y SI +    +SY+ G    QL EK
Sbjct: 711 YGSIAVSVSGISYVVGNFLEQLLEK 729

[Close]

BLAST of HG10022666 vs. ExPASy Swiss-Prot
Match: Q8W4P8 (Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.7e-11
Identity = 49/140 (35.00%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 26  TLEEERVELPRLKKNNPNKGTE-VGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPF 85
           +L+E   E PR + N  +   E  G    Y+E +  + +S +H  IA  SF+ FGL+PP 
Sbjct: 463 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 522

Query: 86  VYALFSFHNTGNK--ELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAIYVSIG 145
           VY  FSF     K  E K++A    SLLC   L+I KA++ +  +   Y+KT   Y +  
Sbjct: 523 VYG-FSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTA 582

Query: 146 IGAFVLSYLAGYMFGQLTEK 163
             A   S   GY+  Q  EK
Sbjct: 583 TTASGFSQFVGYLVSQWLEK 598

[Close]

BLAST of HG10022666 vs. ExPASy TrEMBL
Match: A0A6J1GQF8 (uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.9e-29
Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K+KM+SLL F IA+LSF+ FGLVPP VYAL S
Sbjct: 775 RHDVSRLQKKRKIPRIEVD-GNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSS 834

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P +R++Y+KTAA+ VS+G+GAF +S
Sbjct: 835 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGIS 894

Query: 151 YLAGYMFGQLTEKL 164
           YLAGYMFG L E+L
Sbjct: 895 YLAGYMFGHLAEEL 906

[Close]

BLAST of HG10022666 vs. ExPASy TrEMBL
Match: A0A6J1GQA4 (uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.9e-29
Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K+KM+SLL F IA+LSF+ FGLVPP VYAL S
Sbjct: 776 RHDVSRLQKKRKIPRIEVD-GNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSS 835

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P +R++Y+KTAA+ VS+G+GAF +S
Sbjct: 836 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGIS 895

Query: 151 YLAGYMFGQLTEKL 164
           YLAGYMFG L E+L
Sbjct: 896 YLAGYMFGHLAEEL 907

[Close]

BLAST of HG10022666 vs. ExPASy TrEMBL
Match: A0A6J1GQF7 (uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111456533 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.9e-29
Identity = 77/134 (57.46%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K+KM+SLL F IA+LSF+ FGLVPP VYAL S
Sbjct: 588 RHDVSRLQKKRKIPRIEVD-GNNYKEVLKEKMYSLLCFIIAYLSFLCFGLVPPLVYALSS 647

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++A AGASL CTA LA+QKAHI  +P +R++Y+KTAA+ VS+G+GAF +S
Sbjct: 648 L-KIRNKNLKIIAAAGASLSCTALLAVQKAHINQKPRHRLVYVKTAAVDVSVGVGAFGIS 707

Query: 151 YLAGYMFGQLTEKL 164
           YLAGYMFG L E+L
Sbjct: 708 YLAGYMFGHLAEEL 719

[Close]

BLAST of HG10022666 vs. ExPASy TrEMBL
Match: A0A6J1JQG4 (membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 8.0e-27
Identity = 73/130 (56.15%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 31  RVELPRLKKNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFS 90
           R ++ RL+K       EV   ++YKE +K+KM+ LL F IA+LSF+FFGLVPP VYAL S
Sbjct: 74  RHDVSRLQKKRKIPRIEVD-GNNYKEVLKEKMYRLLCFIIAYLSFLFFGLVPPLVYALSS 133

Query: 91  FHNTGNKELKLVATAGASLLCTAFLAIQKAHIG-RPENRVIYIKTAAIYVSIGIGAFVLS 150
                NK LK++  AGASL CTA LA+QKAHI  +P NR++Y+KTAA+ VS+ +GAF +S
Sbjct: 134 L-KIRNKNLKIIVAAGASLSCTALLAVQKAHINQKPRNRLVYVKTAAVDVSVRVGAFGIS 193

Query: 151 YLAGYMFGQL 160
           YL GYMFG L
Sbjct: 194 YLVGYMFGTL 201

[Close]

BLAST of HG10022666 vs. ExPASy TrEMBL
Match: A0A6J1JXX6 (membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111488504 PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.9e-20
Identity = 62/124 (50.00%), Postives = 78/124 (62.90%), Query Frame = 0


Query: 39  KNNPNKGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFSFHNTGNKE 98
           K  PN+  E  +   Y+E + D+ H LLHF IA LSF+ FGL+PP VY  FSF NT + +
Sbjct: 335 KKEPNERGE-AKVDSYEEALGDRQHYLLHFTIAMLSFLIFGLLPPLVYG-FSFRNTDDGD 394

Query: 99  LKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAIYVSIGIGAFVLSYLAGYMFGQ 158
           LKL A A +SLLC A LAI KA+  R  N   Y KT   Y+++G GA  LSYLAG  F +
Sbjct: 395 LKLAAVAASSLLCIALLAIAKAYAQRARNWQAYAKTLVYYITLGFGASGLSYLAGKEFDK 454

Query: 159 LTEK 163
           L E+
Sbjct: 455 LLEQ 456

[Close]

BLAST of HG10022666 vs. TAIR 10
Match: AT5G24290.2 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 81.3 bits (199), Expect = 8.2e-16
Identity = 45/114 (39.47%), Postives = 68/114 (59.65%), Query Frame = 0


Query: 54  YKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTA 113
           Y+E +  +  S +H  +A +S+IFFGL+PP VYA FSF+ TG K  KL++    SL+C  
Sbjct: 395 YEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYA-FSFYETGIKNYKLISVFLGSLVCVI 454

Query: 114 FLAIQKAHIGRPENRV----IYIKTAAIYVSIGIGAFVLSYLAGYMFGQLTEKL 164
            L   K ++ +P N       Y+K+AA Y SI + +  +SY+ G + G+  EKL
Sbjct: 455 LLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKL 507

[Close]

BLAST of HG10022666 vs. TAIR 10
Match: AT5G24290.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 81.3 bits (199), Expect = 8.2e-16
Identity = 45/114 (39.47%), Postives = 68/114 (59.65%), Query Frame = 0


Query: 54  YKEEVKDKMHSLLHFAIAFLSFIFFGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTA 113
           Y+E +  +  S +H  +A +S+IFFGL+PP VYA FSF+ TG K  KL++    SL+C  
Sbjct: 411 YEELLGRRTKSRIHILVAVMSYIFFGLIPPLVYA-FSFYETGIKNYKLISVFLGSLVCVI 470

Query: 114 FLAIQKAHIGRPENRV----IYIKTAAIYVSIGIGAFVLSYLAGYMFGQLTEKL 164
            L   K ++ +P N       Y+K+AA Y SI + +  +SY+ G + G+  EKL
Sbjct: 471 LLGSIKVYVRKPTNSCGSTKAYLKSAAYYTSIVVASCGISYVVGDIMGEYIEKL 523

[Close]

BLAST of HG10022666 vs. TAIR 10
Match: AT4G27870.1 (Vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.0e-14
Identity = 55/145 (37.93%), Postives = 75/145 (51.72%), Query Frame = 0


Query: 19  LMVEPLKTLEEERVELPRLKKNNPN-KGTEVGRWSHYKEEVKDKMHSLLHFAIAFLSFIF 78
           L++  L+ L EE       + N  N +  E GR   YK  +  + +  LH  +A LSFI 
Sbjct: 591 LIIHNLQELREEEPIRTTTEDNQTNGREEEEGR---YKRLLGRRENFTLHATVAILSFII 650

Query: 79  FGLVPPFVYALFSFHNTGNKELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAI 138
            G++PP VY  FSF    NK+ K+ +  GASL C   LAI KAH+  P  R  Y+K+   
Sbjct: 651 TGILPPVVY-YFSFSEKHNKDYKVASVFGASLFCIVLLAIAKAHVRYP--RGSYLKSILY 710

Query: 139 YVSIGIGAFVLSYLAGYMFGQLTEK 163
           Y SI +    +SY+ G    QL EK
Sbjct: 711 YGSIAVSVSGISYVVGNFLEQLLEK 729

[Close]

BLAST of HG10022666 vs. TAIR 10
Match: AT4G27860.1 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 68.6 bits (166), Expect = 5.5e-12
Identity = 49/140 (35.00%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 26  TLEEERVELPRLKKNNPNKGTE-VGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPF 85
           +L+E   E PR + N  +   E  G    Y+E +  + +S +H  IA  SF+ FGL+PP 
Sbjct: 463 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 522

Query: 86  VYALFSFHNTGNK--ELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAIYVSIG 145
           VY  FSF     K  E K++A    SLLC   L+I KA++ +  +   Y+KT   Y +  
Sbjct: 523 VYG-FSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTA 582

Query: 146 IGAFVLSYLAGYMFGQLTEK 163
             A   S   GY+  Q  EK
Sbjct: 583 TTASGFSQFVGYLVSQWLEK 598

[Close]

BLAST of HG10022666 vs. TAIR 10
Match: AT4G27860.2 (vacuolar iron transporter (VIT) family protein )

HSP 1 Score: 68.6 bits (166), Expect = 5.5e-12
Identity = 49/140 (35.00%), Postives = 70/140 (50.00%), Query Frame = 0


Query: 26  TLEEERVELPRLKKNNPNKGTE-VGRWSHYKEEVKDKMHSLLHFAIAFLSFIFFGLVPPF 85
           +L+E   E PR + N  +   E  G    Y+E +  + +S +H  IA  SF+ FGL+PP 
Sbjct: 448 SLQELINEKPRKQTNTDDSPEEGEGEEDRYEEVLGRREYSRIHRVIAISSFVIFGLIPPL 507

Query: 86  VYALFSFHNTGNK--ELKLVATAGASLLCTAFLAIQKAHIGRPENRVIYIKTAAIYVSIG 145
           VY  FSF     K  E K++A    SLLC   L+I KA++ +  +   Y+KT   Y +  
Sbjct: 508 VYG-FSFRKKMEKRQEYKVLAVYAVSLLCIVLLSIAKAYVSKKRD---YVKTLFRYTTTA 567

Query: 146 IGAFVLSYLAGYMFGQLTEK 163
             A   S   GY+  Q  EK
Sbjct: 568 TTASGFSQFVGYLVSQWLEK 583

The following BLAST results are available for this feature:

BLAST of HG10022666 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023548918.1	2.5e-30	58.21	uncharacterized protein LOC111807425 isoform X3 [Cucurbita pepo subsp. pepo]	[more]
XP_023548919.1	2.5e-30	58.21	uncharacterized protein LOC111807425 isoform X4 [Cucurbita pepo subsp. pepo]	[more]
XP_023548916.1	2.5e-30	58.21	uncharacterized protein LOC111807425 isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_023548920.1	2.5e-30	58.21	uncharacterized protein LOC111807425 isoform X5 [Cucurbita pepo subsp. pepo]	[more]
XP_023548921.1	2.5e-30	58.21	uncharacterized protein LOC111807425 isoform X6 [Cucurbita pepo subsp. pepo]	[more]

BLAST of HG10022666 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KFS7	1.2e-14	39.47	Membrane protein of ER body 2 OS=Arabidopsis thaliana OX=3702 GN=MEB2 PE=1 SV=1	[more]
Q8LPT3	2.8e-13	37.93	Membrane protein of ER body-like protein OS=Arabidopsis thaliana OX=3702 GN=MEBL...	[more]
Q8W4P8	7.7e-11	35.00	Membrane protein of ER body 1 OS=Arabidopsis thaliana OX=3702 GN=MEB1 PE=1 SV=1	[more]

BLAST of HG10022666 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GQF8	2.9e-29	57.46	uncharacterized protein LOC111456533 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GQA4	2.9e-29	57.46	uncharacterized protein LOC111456533 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GQF7	2.9e-29	57.46	uncharacterized protein LOC111456533 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1JQG4	8.0e-27	56.15	membrane protein of ER body 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488012 P...	[more]
A0A6J1JXX6	1.9e-20	50.00	membrane protein of ER body-like protein isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]

BLAST of HG10022666 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24290.2	8.2e-16	39.47	Vacuolar iron transporter (VIT) family protein	[more]
AT5G24290.1	8.2e-16	39.47	Vacuolar iron transporter (VIT) family protein	[more]
AT4G27870.1	2.0e-14	37.93	Vacuolar iron transporter (VIT) family protein	[more]
AT4G27860.1	5.5e-12	35.00	vacuolar iron transporter (VIT) family protein	[more]
AT4G27860.2	5.5e-12	35.00	vacuolar iron transporter (VIT) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31851:SF64	GLYCINE-RICH PROTEIN FAMILY	coord: 47..163
IPR008217	Ccc1 family	PANTHER	PTHR31851	FE(2+)/MN(2+) TRANSPORTER PCL1	coord: 47..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022666.1	HG10022666.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030026	cellular manganese ion homeostasis
cellular_component	GO:0016020	membrane
molecular_function	GO:0005384	manganese ion transmembrane transporter activity