HG10022581 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022581 vs. NCBI nr
Match: XP_038897996.1 (eukaryotic translation initiation factor 5B [Benincasa hispida])

HSP 1 Score: 1864.4 bits (4828), Expect = 0.0e+00
Identity = 1092/1342 (81.37%), Postives = 1120/1342 (83.46%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQV+EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQVQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDDDDDVDGVSEIVITGKKKGK+KKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDDDDVDGVSEIVITGKKKGKTKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVIE 180
            GAD+DD SVLTAEKDDDEE DSAIKFSGKKKSSKSSKKSGFSAVSAF+ALDDE DEDV +
Sbjct: 121  GADEDDGSVLTAEKDDDEEVDSAIKFSGKKKSSKSSKKSGFSAVSAFSALDDEKDEDVND 180

Query: 181  NEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTSI 240
            NEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFT FSGLDYEDEDRDDDKD DEDV SI
Sbjct: 181  NEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTAFSGLDYEDEDRDDDKDEDEDVASI 240

Query: 241  SFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGKK 300
            SFSGKKKKSAK SKKSGN F+AALADEEND  VSISEPNKLDNDG  EDDVNVIAFSGKK
Sbjct: 241  SFSGKKKKSAKASKKSGNSFSAALADEENDGGVSISEPNKLDNDG--EDDVNVIAFSGKK 300

Query: 301  KSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKSK 360
            KSSKKKS+STFTA+ DENAQGNEVKDVVVPEILNTVSSNLDSDLSNA+K EGV ETSK+K
Sbjct: 301  KSSKKKSSSTFTALSDENAQGNEVKDVVVPEILNTVSSNLDSDLSNASKMEGVVETSKNK 360

Query: 361  KKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAEE 420
            KKKKKSGRTAQEEDDLDKILAELGEGPT+SKPADPPLSSQEA+VENPPELVAPPEKEAEE
Sbjct: 361  KKKKKSGRTAQEEDDLDKILAELGEGPTISKPADPPLSSQEAKVENPPELVAPPEKEAEE 420

Query: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEKK 480
            ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEV TEIIEPKKGAAKSKVPEKK
Sbjct: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVNTEIIEPKKGAAKSKVPEKK 480

Query: 481  VPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREKE 540
            VPKHVREMQEAMARRKEEEERRKREEEERLRKEEEER RLEELERQAEEAKRRKKEREKE
Sbjct: 481  VPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERLRLEELERQAEEAKRRKKEREKE 540

Query: 541  KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTKP 600
            KLLRKK EGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKK KP
Sbjct: 541  KLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKAKP 600

Query: 601  AHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNEI 660
            AHHQ NGNAQTKA+ H+EEKIQEKDVAETEILESEK+EAVELMHVEEKSDVIEATEDNEI
Sbjct: 601  AHHQPNGNAQTKAVGHVEEKIQEKDVAETEILESEKVEAVELMHVEEKSDVIEATEDNEI 660

Query: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKGL 720
            QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNG GAKLAAP QKGL
Sbjct: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGTGAKLAAPVQKGL 720

Query: 721  PSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMGH 780
            PSQP+KSQDI+NKKKQHEVEVADKGK KDDAVKKKASIPDATPV QEENLRSPICCIMGH
Sbjct: 721  PSQPVKSQDIDNKKKQHEVEVADKGKGKDDAVKKKASIPDATPVQQEENLRSPICCIMGH 780

Query: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840
            VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII
Sbjct: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840

Query: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900
            DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL
Sbjct: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900

Query: 901  YGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK---------------------- 960
            YGWKTIRNAPILKTMKQQ+KDVQNEFNMRLIQ   +FK                      
Sbjct: 901  YGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIV 960

Query: 961  ----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNGV 1020
                       D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNGV
Sbjct: 961  PTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGV 1020

Query: 1021 LHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080
            LHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA
Sbjct: 1021 LHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080

Query: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT------------------------ 1140
            IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                        
Sbjct: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKSP 1140

Query: 1141 --------------------------------------------------DDTYKKVSID 1164
                                                              D+   K+ I 
Sbjct: 1141 AVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFIA 1200

BLAST of HG10022581 vs. NCBI nr
Match: KAA0050994.1 (eukaryotic translation initiation factor 5B-like [Cucumis melo var. makuwa])

HSP 1 Score: 1831.6 bits (4743), Expect = 0.0e+00
Identity = 1076/1342 (80.18%), Postives = 1117/1342 (83.23%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKK+ STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KKKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAPPEKEAE
Sbjct: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPPEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQEKDVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKGKRK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1163
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. NCBI nr
Match: TYK04381.1 (eukaryotic translation initiation factor 5B-like [Cucumis melo var. makuwa])

HSP 1 Score: 1831.6 bits (4743), Expect = 0.0e+00
Identity = 1076/1342 (80.18%), Postives = 1117/1342 (83.23%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKK+ STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KKKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAPPEKEAE
Sbjct: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPPEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQEKDVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKGKRK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1163
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. NCBI nr
Match: XP_008461514.2 (PREDICTED: LOW QUALITY PROTEIN: eukaryotic translation initiation factor 5B-like [Cucumis melo])

HSP 1 Score: 1827.0 bits (4731), Expect = 0.0e+00
Identity = 1074/1343 (79.97%), Postives = 1116/1343 (83.10%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKKS STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKSNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KK KKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAP EKEAE
Sbjct: 361  KKXKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPAEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQE+DVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQERDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKG+RK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGERKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1164
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. NCBI nr
Match: XP_011659144.1 (eukaryotic translation initiation factor 5B [Cucumis sativus] >KGN44524.1 hypothetical protein Csa_015960 [Cucumis sativus])

HSP 1 Score: 1823.5 bits (4722), Expect = 0.0e+00
Identity = 1068/1342 (79.58%), Postives = 1113/1342 (82.94%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+K+ DDD+DDVDGVSEIVITGKKKGKSKKGGSSSAF++SSFGLLEEEG+D
Sbjct: 61   GKKGNSKASQLKD-DDDEDDVDGVSEIVITGKKKGKSKKGGSSSAFSSSSFGLLEEEGID 120

Query: 121  GADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVIE 180
             ADDD++SVLT EKDDDEEE SAIKFSGKKKSSKSSKKSGFSAVSAFTALDD+NDED I+
Sbjct: 121  DADDDEESVLTTEKDDDEEEHSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDDNDEDAID 180

Query: 181  NEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTSI 240
            NEIR DEDID EPVI FTGKKKSSKGGKKAGSAF+GFSGLDYEDEDRDD KD +EDVTSI
Sbjct: 181  NEIRADEDIDGEPVIEFTGKKKSSKGGKKAGSAFSGFSGLDYEDEDRDDKKD-EEDVTSI 240

Query: 241  SFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGKK 300
            SFSGKKKKSAK SKKSGN F+AALADEEND D S+SE NKLD+DGVNEDD+NVIAFSGKK
Sbjct: 241  SFSGKKKKSAKASKKSGNSFSAALADEENDGDFSMSETNKLDHDGVNEDDLNVIAFSGKK 300

Query: 301  KSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKSK 360
            KSSKKKS ST TA+ DENAQ NE KDVVVPEI NTVSSNLDSDLSNANKTE VAETSK+K
Sbjct: 301  KSSKKKSNSTVTALSDENAQANEAKDVVVPEIHNTVSSNLDSDLSNANKTEAVAETSKNK 360

Query: 361  KKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAEE 420
            KKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL  QEA+VENPPELVAPPEKEAEE
Sbjct: 361  KKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLFFQEAKVENPPELVAPPEKEAEE 420

Query: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEKK 480
            ESTESAAARKKKKKKEKEKEKKAAAAAAAAEG+DEK+EEVK+EIIEPKKGAAKSKVPEKK
Sbjct: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGSDEKVEEVKSEIIEPKKGAAKSKVPEKK 480

Query: 481  VPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREKE 540
            VPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREKE
Sbjct: 481  VPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREKE 540

Query: 541  KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTKP 600
            KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILS+AGGLPLSTSDPSAPAKRPKYQTKKTKP
Sbjct: 541  KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKP 600

Query: 601  AHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNEI 660
            +HHQTNGNAQTK +EHI EKIQEKDVAETE+LESEKIEAVELMHVEEKS V+EATEDNEI
Sbjct: 601  SHHQTNGNAQTKVVEHIVEKIQEKDVAETEVLESEKIEAVELMHVEEKSGVLEATEDNEI 660

Query: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKGL 720
            QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAP+QKGL
Sbjct: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPSQKGL 720

Query: 721  PSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMGH 780
            PSQ IKSQDIENKKKQ  VEVADKGKRK+DAV+KKASI DATPV QEENLRSPICCIMGH
Sbjct: 721  PSQSIKSQDIENKKKQDGVEVADKGKRKEDAVRKKASISDATPVQQEENLRSPICCIMGH 780

Query: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840
            VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII
Sbjct: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840

Query: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900
            DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL
Sbjct: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900

Query: 901  YGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK---------------------- 960
            YGWK+IRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                      
Sbjct: 901  YGWKSIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIV 960

Query: 961  ----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNGV 1020
                       D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNGV
Sbjct: 961  PTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGV 1020

Query: 1021 LHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080
            LHEGDQIV     GPIVT+IRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA
Sbjct: 1021 LHEGDQIVVCGMQGPIVTSIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080

Query: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT------------------------ 1140
            IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                        
Sbjct: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKSP 1140

Query: 1141 --------------------------------------------------DDTYKKVSID 1164
                                                              D+   K+ I 
Sbjct: 1141 AVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFIA 1200

BLAST of HG10022581 vs. ExPASy Swiss-Prot
Match: G0S8G9 (Eukaryotic translation initiation factor 5B OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) OX=759272 GN=CTHT_0029840 PE=1 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 1.3e-115
Identity = 385/1059 (36.36%), Postives = 512/1059 (48.35%), Query Frame = 0

Query: 358  KSKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKE 417
            K+K+K+KK G +   ED LD    E  EG   +   +P LS+++A+  N  +  A P+K+
Sbjct: 58   KNKEKRKKKGLS---EDWLD---GETPEGQAPAAAPEPDLSAKQAEEANLEDEWALPDKK 117

Query: 418  AEE-------------------------------ESTESAAARKKKKKKEKEKEKKAAAA 477
             ++                               E       R+K++KKE+  +KKAA  
Sbjct: 118  GKKGGKQQQNKKQQQEEKKKDDEEVPEIRVLTKAEKERLKKEREKQRKKEQAAKKKAAGP 177

Query: 478  AAAAEGNDEKIEEVKTEIIEPKKGAAKSKVP-----EKKVPKHVR---EMQEAMARRKEE 537
            A   +    K  E + +  EP    A +  P     +KK+P H+R   + QE + RR+EE
Sbjct: 178  AQPQKAEPAKPAEEEKKPEEPAPAPAPAPEPAAGGSKKKIPAHLRLIQKQQEELRRRQEE 237

Query: 538  EERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREKEKLLRKKLEGKLLTGKQKE 597
            E+RR  EE  R+   EEE RR EE  ++ EE K RKK++EKEK+ + K EGK LT  Q+E
Sbjct: 238  EQRRLEEERRRI---EEEERRAEEERKRKEEEKARKKQKEKEKIEQLKREGKYLTKAQRE 297

Query: 598  E----QRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTKPAHHQTNGNAQTKAM 657
            E    QR LE MR   +  A     +  + +A  ++ K + KK +        + Q KA+
Sbjct: 298  EKARNQRMLEQMRAAGIKIAALEEKNKEEGAAEGEKEKKEKKKPEKKRRPNKVDEQ-KAL 357

Query: 658  EHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNEIQED-----EDEDEW 717
            E   E+ +++  AE    E+E+   +E    E +    +A  +  + ED     E + E 
Sbjct: 358  EEAAERARQQ--AEAAAREAEEKARLEREKAEAEEKAKQAAAEESVDEDWEAAAESDKED 417

Query: 718  DAKSWDDAVVDLSLKSSFADEELESE--PENGMKKDTKNGAGAKLAAPAQKGLPSQPIKS 777
               SWD A  D   +    +EE E +  P+   KK        K A P +     + +K 
Sbjct: 418  VPDSWDAAASDEEKEEEEEEEEEEEKPAPKKTEKKPEPKTEEKKKAEPKKPEAKKEEVKK 477

Query: 778  QDIENKKKQHEVEVADKGK----------------RKDDAVKKKASIPDATPVPQEENLR 837
             +    KK+   +    GK                +  +A KK+ +     PV  ++NLR
Sbjct: 478  PE---PKKEEPKKAEANGKPATTTLPTRSKEEEKPKAAEAEKKEVAARPKKPVVNKDNLR 537

Query: 838  SPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAK 897
            SPICCI+GHVDTGKTKLLD IR TNVQEGEAGGITQQIGATYFP E I+++T  +  D K
Sbjct: 538  SPICCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATYFPVEAIKQKTAVVNKDGK 597

Query: 898  --LKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTE 957
               KVPGLLIIDTPGHESF+NLRSRGS LC++AILVVDIMHGLEPQTIESL LLR R T 
Sbjct: 598  FEFKVPGLLIIDTPGHESFSNLRSRGSSLCNIAILVVDIMHGLEPQTIESLRLLRERKTP 657

Query: 958  FIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQKKFK------------- 1017
            F+VALNK+DRLYGWK I N    ++   Q K VQNEF  RL Q K +             
Sbjct: 658  FVVALNKIDRLYGWKKIENNGFRESFALQNKAVQNEFRNRLDQVKLQFAEQGFNSELFYE 717

Query: 1018 ---------------------GDHILIVHQQC----------------TVLEVKVVEGHG 1077
                                  D + ++ Q C                TVLEVK +EG G
Sbjct: 718  NKNFARYVSLVPTSAHTGEGIPDMLKLIVQLCQERMASSLMYLSELQATVLEVKAIEGFG 777

Query: 1078 TTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQG 1137
             TIDVIL NG+L EGD+IV     GPI T IRALLTP PM+ELR+KG Y+HHKE+KAAQG
Sbjct: 778  VTIDVILSNGILREGDRIVLCGLEGPIKTNIRALLTPAPMRELRIKGQYIHHKEVKAAQG 837

Query: 1138 IKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT------------- 1164
            +KI+  GLE AIAG+ L VVGP+DD E++++    D++S+ SR++KT             
Sbjct: 838  VKISAPGLEGAIAGSRLLVVGPDDDEEELEEEVESDLQSLFSRVEKTGKGVSVQASTLGS 897

BLAST of HG10022581 vs. ExPASy Swiss-Prot
Match: P39730 (Eukaryotic translation initiation factor 5B OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=FUN12 PE=1 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 3.7e-102
Identity = 356/1062 (33.52%), Postives = 493/1062 (46.42%), Query Frame = 0

Query: 300  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 359
            KKS K +         ++ AQ  E+     P   ++  ++ D+    +   EG       
Sbjct: 3    KKSKKNQQNYWDEEFEEDAAQNEEISATPTPNPESSAGAD-DTSREASASAEGAEAIEGD 62

Query: 360  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 419
                 K  +  QE+    K++ E  +G  + K      S +E + E         +KE E
Sbjct: 63   FMSTLKQSKKKQEK----KVIEEKKDGKPILK------SKKEKEKE---------KKEKE 122

Query: 420  EESTESAAARKK-----KKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKS 479
            ++  +  AARKK     +K+K KE  K+    AAA +   EK ++ K E  +P   +A +
Sbjct: 123  KQKKKEQAARKKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESDKP---SASA 182

Query: 480  KVPEKKVPKHVREMQEAMARRK--EEEERRKREEEERLRKEEEERRRLEELERQAEEAKR 539
            K P KKVP  +  ++  +  +K  EE+E+ +REEEERL KEEEE  RL   E+  EEAK 
Sbjct: 183  KKPAKKVPAGLAALRRQLELKKQLEEQEKLEREEEERLEKEEEE--RLANEEKMKEEAKA 242

Query: 540  RKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSS----AGGLPLSTSDPSAPA 599
             KKE+EK K  ++K EGKLLT KQKEE++ LE  R  +LSS      GL     + + P 
Sbjct: 243  AKKEKEKAKREKRKAEGKLLTRKQKEEKKLLERRRAALLSSGNVKVAGLAKKDGEENKP- 302

Query: 600  KRPKYQTKKTKPAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEK 659
            K+  Y  KK +               E+  E I+     ++E++                
Sbjct: 303  KKVVYSKKKKR------------TTQENASEAIKSDSKKDSEVV---------------- 362

Query: 660  SDVIEATEDNEIQEDEDE--DEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKN 719
                    D+E++E ED   D+W+  +  D                  + E G  ++T+ 
Sbjct: 363  -------PDDELKESEDVLIDDWENLALGD------------------DDEEGTNEETQE 422

Query: 720  GAGAKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPV-- 779
               +       +G   +  + ++ E +++ H  EVA             A+ P ATP   
Sbjct: 423  STASHENEDQNQGEEEEEGEEEEEEEEERAHVHEVAK---------STPAATPAATPTPS 482

Query: 780  ---PQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRE 839
               P +++LRSPICCI+GHVDTGKTKLLD IR TNVQ GEAGGITQQIGATYFP + I+ 
Sbjct: 483  SASPNKKDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIDAIKA 542

Query: 840  RTRELKADAK--LKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIES 899
            +T+ +    K    VPGLL+IDTPGHESF+NLRSRGS LC++AILV+DIMHGLE QTIES
Sbjct: 543  KTKVMAEYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIES 602

Query: 900  LNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMR---------- 959
            + LLR R   F+VALNK+DRLY WK I N     +  +Q++ VQ EF  R          
Sbjct: 603  IKLLRDRKAPFVVALNKIDRLYDWKAIPNNSFRDSFAKQSRAVQEEFQSRYSKIQLELAE 662

Query: 960  ----------------------------------------LIQKKFKGDHILIVHQQCTV 1019
                                                    L QK+     + + H + T+
Sbjct: 663  QGLNSELYFQNKNMSKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLMYLSHVEATI 722

Query: 1020 LEVKVVEGHGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYL 1079
            LEVKVVEG GTTIDVIL NG L EGD+IV     GPIVT IRALLTP P++ELR+K  Y+
Sbjct: 723  LEVKVVEGFGTTIDVILSNGYLREGDRIVLCGMNGPIVTNIRALLTPQPLRELRLKSEYV 782

Query: 1080 HHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT--- 1139
            HHKE+KAA G+KI    LE A++G+ L VVGPEDD +++ D  M+D+  +L  +D T   
Sbjct: 783  HHKEVKAALGVKIAANDLEKAVSGSRLLVVGPEDDEDELMDDVMDDLTGLLDSVDTTGKG 842

Query: 1140 ------------------------------DDTYKK----------------------VS 1159
                                             YK+                      V 
Sbjct: 843  VVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKASTMLEKAPEYAVMLCFDVK 902

BLAST of HG10022581 vs. ExPASy Swiss-Prot
Match: Q10251 (Eukaryotic translation initiation factor 5B OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC56F8.03 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.7e-99
Identity = 374/1129 (33.13%), Postives = 521/1129 (46.15%), Query Frame = 0

Query: 251  KTSKKSGNLFTAALADEENDDDVSISEPNKL---------DNDGVNEDDVNVIAFSGK-- 310
            K  KKSG       AD E+D    IS  N+           ND + E   N+   S K  
Sbjct: 3    KKGKKSG------YADWEDDLGEDISGQNEYLDNTSQDSPQNDELAEKSENLAVSSEKTT 62

Query: 311  -KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSK 370
             KK   KK+      + D+ +Q  E++    P+ L  V+   D +     K         
Sbjct: 63   SKKKKGKKNKGNKNQVSDDESQ--ELESPQGPKELTAVTELDDDEFDYKPK--------- 122

Query: 371  SKKKKKKSGRTAQEEDDLDKI-----------LAELGEGPTVSKPADPPLSSQEAQVENP 430
             K KK K  +  +E+D+  +I           + EL +     KP       ++AQ  N 
Sbjct: 123  -KGKKGKKSKKVEEDDEPQEIESPQGPKELTAVTELDDDEFDYKPKKGK-KGKKAQNNNE 182

Query: 431  PELVAPPE------KEAEEESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVK 490
             E  APPE      K  +E+  E     K +KK+++ K+K +      A        ++ 
Sbjct: 183  SEAAAPPEIPEVRVKTKKEKEREKKEREKLRKKQQQAKKKGSTGEDTLASSEVSSEVDIS 242

Query: 491  TEIIEPKKGAAKSKVPEKKVPKHVREMQEAM--ARRKEEEERRKREEEERLRKEEEERRR 550
            T          K     K+   +V  +Q+ +   R +EEEE+R REEE R+   EEE+R 
Sbjct: 243  TPAENDSSAKGKQAAGSKRKGPNVTALQKMLEEKRAREEEEQRIREEEARI--AEEEKRL 302

Query: 551  LEELERQAEEAKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPL 610
             E  E + EEA+ +KKE+E++K    K +GK L+ KQKE+Q   +    Q+L S  G+ +
Sbjct: 303  AEVEEARKEEARLKKKEKERKKKEEMKAQGKYLSKKQKEQQALAQRRLQQMLES--GVRV 362

Query: 611  STSDPSAPAKRPKYQTKK----TKPAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESE 670
            +        ++P Y  KK    +  +   ++G  ++     I     +KD  +    +SE
Sbjct: 363  AGLSNGEKKQKPVYTNKKKSNRSGTSSISSSGILESSPATSISVDEPQKDSKD----DSE 422

Query: 671  KIEAVELMHVEEKSDVIEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESE 730
            K+         EK   +E  E+NE + +   D+W+A   +  V +        + E+ +E
Sbjct: 423  KV---------EKETEVERKEENEAEAEAVFDDWEAALEEPEVAE--------NNEVVTE 482

Query: 731  PENGMKKDTKNGAGAKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKK 790
                 KK+T                    IKS  +E+  K  E          D    K 
Sbjct: 483  -----KKET-------------------DIKSDAVEHSIKDKE----------DSKTDKV 542

Query: 791  ASIPDATPVP---QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGAT 850
              IP A P      E +LRSPICCI+GHVDTGKTKLLD +R +NVQEGEAGGITQQIGAT
Sbjct: 543  DDIPQAAPAESNVSESDLRSPICCILGHVDTGKTKLLDNLRRSNVQEGEAGGITQQIGAT 602

Query: 851  YFPAENIRERTRELKADAKLK--VPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMH 910
            YFP E+I+++T+ +    KL+  +PGLLIIDTPGHESFTNLRSRG+ LC++AILV+DIMH
Sbjct: 603  YFPIESIKQKTKVVNKKGKLQYNIPGLLIIDTPGHESFTNLRSRGTSLCNIAILVIDIMH 662

Query: 911  GLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRL 970
            GLEPQTIES+ LLR + T F+VALNKVDRLYGW +I++  I  ++ +Q K +Q EF  R+
Sbjct: 663  GLEPQTIESIRLLRDQKTPFVVALNKVDRLYGWHSIKDNAIQDSLSKQKKAIQREFRDRV 722

Query: 971  -----------------IQKKFKGDHILIVHQ---------------------------- 1030
                              + K  G ++ +V                              
Sbjct: 723  ESIILQLNEQGLNAALYFENKNLGRYVSLVPTSAQSGEGVPDLVALLISLTQTRMSDRIK 782

Query: 1031 -----QCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMK 1090
                 +CTVLEVKV+EG G TIDVIL NGVLHEGD+IV     GPI+TT+RALLTP P+K
Sbjct: 783  YITTLECTVLEVKVIEGLGATIDVILSNGVLHEGDRIVLCGMGGPIITTVRALLTPQPLK 842

Query: 1091 ELRVKGTYLHHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVL 1150
            E+RVK  Y+HHKEIKAA G+KI    LE A+AG+ L VVGP+DD ED+ +  MED++++L
Sbjct: 843  EMRVKSAYVHHKEIKAAMGVKICANDLEKAVAGSRLLVVGPDDDEEDLAEEIMEDLENLL 902

Query: 1151 SRIDKT---------------------------------DDTYKK--------------- 1159
             RID +                                    YKK               
Sbjct: 903  GRIDTSGIGVSVQASTLGSLEALLEFLKQMKIPVASVNIGPVYKKDVMRCATMLEKAKEY 962

BLAST of HG10022581 vs. ExPASy Swiss-Prot
Match: O60841 (Eukaryotic translation initiation factor 5B OS=Homo sapiens OX=9606 GN=EIF5B PE=1 SV=4)

HSP 1 Score: 364.4 bits (934), Expect = 5.1e-99
Identity = 417/1326 (31.45%), Postives = 600/1326 (45.25%), Query Frame = 0

Query: 61   GKKGNSKASQIKENDDDDDDVDGV-SEIVITG--------KKKGKSKKGGSSSAFTASS- 120
            GKK  +K+   +++  DD D+D + +EI   G        K KGK KK      F     
Sbjct: 2    GKKQKNKS---EDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDI 61

Query: 121  FGLLEEEGMD--GADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFT 180
               LEE  ++  G   D ++V     +++EEE ++     KKK  +  KK  F       
Sbjct: 62   LKELEELSLEAQGIKADRETVAVKPTENNEEEFTS---KDKKKKGQKGKKQSF------- 121

Query: 181  ALDDENDEDVIENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRD 240
               D+ND + +E+                    K SK  K A      +SG         
Sbjct: 122  ---DDNDSEELED--------------------KDSKSKKTAKPKVEMYSG--------- 181

Query: 241  DDKDGDEDVTSISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNE 300
               D D+D   +    K K      K  G       ++E+ D+   I E +++++ G + 
Sbjct: 182  --SDDDDDFNKLPKKAKGKAQKSNKKWDG-------SEEDEDNSKKIKERSRINSSGESG 241

Query: 301  DDVNVIAFS--GKKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSD--- 360
            D+ +    S  G+KK+ K K      +  +++    ++K V   +           D   
Sbjct: 242  DESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEK 301

Query: 361  --LSNANKTEGVAETSKSKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQE 420
              L    + E +    K + K+K+S R  +EE    K+  + G  P   + A+ P     
Sbjct: 302  AKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETP----- 361

Query: 421  AQVENPPELVAPPEKEAEEESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVK 480
                            A E+  E    +K KKKK+ EKE+K            EK     
Sbjct: 362  ---------------TAAEDDNEGDKKKKDKKKKKGEKEEK------------EK----- 421

Query: 481  TEIIEPKKGAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLE 540
                E KKG +K+          V+ MQEA+A+ KEEEER+KREEEER+++ EE   + +
Sbjct: 422  ----EKKKGPSKAT---------VKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRK 481

Query: 541  ELERQAEEAKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLST 600
            E ER  +E + RKK++EKE+  R K EGKLLT  Q+E + R EA     L  A G+ + +
Sbjct: 482  EEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLK--LLQAQGVEVPS 541

Query: 601  SDPSAPAKRPKYQTKKTKPAHHQTNGNAQTKAM------EHIEEKIQEKDVA-------E 660
             D S P KRP Y+ KK K    Q      +++M      E +E+ + EK+         E
Sbjct: 542  KD-SLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEE 601

Query: 661  TEILESEKIEAVELMHVEEKSDVIEATE--------DNEIQEDEDEDEWDAKSWDDAVVD 720
             E  E   ++  E M  +E+++ +E  +          E +E+E+E+E D +S ++    
Sbjct: 602  EEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEE---- 661

Query: 721  LSLKSSFADEELESEPENGMKKDTK----NGAGAKLAAPAQKGLPSQPIKSQDIENKKKQ 780
                    +EE ESE   G ++D K      +G  L     K + S      D +  K  
Sbjct: 662  -------EEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTK-- 721

Query: 781  HEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMGHVDTGKTKLLDCIRGTN 840
             E    DK KR+ +  + + S    T     E LR+PI C++GHVDTGKTK+LD +R T+
Sbjct: 722  -EERAYDKAKRRIEKRRLEHSKNVNT-----EKLRAPIICVLGHVDTGKTKILDKLRHTH 781

Query: 841  VQEGEAGGITQQIGATYFPAENIRERTRELK--ADAKLKVPGLLIIDTPGHESFTNLRSR 900
            VQ+GEAGGITQQIGAT  P E I E+T+ +K      +++PG+LIIDTPGHESF+NLR+R
Sbjct: 782  VQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNR 841

Query: 901  GSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKT 960
            GS LCD+AILVVDIMHGLEPQTIES+NLL+ +   FIVALNK+DRLY WK   ++ +  T
Sbjct: 842  GSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAAT 901

Query: 961  MKQQTKDVQNEFNMR----LIQKKFKGDHILIVHQ------------------------- 1020
            +K+Q K+ ++EF  R    +++   +G +  + ++                         
Sbjct: 902  LKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLI 961

Query: 1021 ---------------------QCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIV-----G 1080
                                 +  V+EVK + G GTTIDVIL+NG L EGD I+     G
Sbjct: 962  YLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEG 1021

Query: 1081 PIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDD 1140
            PIVT IR LL P PMKELRVK  Y  HKE++AAQG+KI G+ LE  +AG  L V   ED+
Sbjct: 1022 PIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDE 1081

Query: 1141 LEDIKDSAMEDMKSVLSRID-----------------------KTDDT------------ 1164
            +  +KD  + ++K  L+ I                        KT +             
Sbjct: 1082 IPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHK 1141

BLAST of HG10022581 vs. ExPASy Swiss-Prot
Match: B2GUV7 (Eukaryotic translation initiation factor 5B OS=Rattus norvegicus OX=10116 GN=Eif5b PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 3.1e-96
Identity = 410/1316 (31.16%), Postives = 594/1316 (45.14%), Query Frame = 0

Query: 61   GKKGNSKASQIKENDDD------DDDVDGVSEIVITGKKKGKSKKGGSSSAFTASS-FGL 120
            GKK  +K+    ++D D      + +  G ++     K KGK KK      F  +     
Sbjct: 2    GKKQKNKSEDSTKDDTDLGALAAEIEGAGAAKEQEPQKGKGKKKKEKKKQDFDENDILRE 61

Query: 121  LEEEGMD----GADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTA 180
            LEE  ++    GAD D     T +  ++ EE+SA K   KKK  +  KK+ F        
Sbjct: 62   LEELSLEAQGIGADRD---AATVKPTENNEEESASK-QDKKKKGQKGKKTSF-------- 121

Query: 181  LDDENDEDVIENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDD 240
              DEND + +E     D+D             KS K  +         S      ED DD
Sbjct: 122  --DENDSEELE-----DKD------------SKSKKPARPNSEVLLSGS------EDADD 181

Query: 241  DKDGDEDVTSISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVN-- 300
                         S K KK+ K++KK         ++E+ D+     E +++++ G +  
Sbjct: 182  PN---------KLSKKGKKAQKSTKKRDG------SEEDEDNSKRSKERSRVNSSGESGG 241

Query: 301  EDDVNVIAFSGKKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNA 360
            E D  + +  G+KK+ K KS  T  +  +++    ++K V                    
Sbjct: 242  ESDEFLQSRKGQKKNQKNKSVPTIDSGNEDDDSSFKIKTVA------------------Q 301

Query: 361  NKTEGVAETSKSKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENP 420
             K E      K ++++K   R  +E+++L+K   E  +     K  D     +   +   
Sbjct: 302  KKAEKKERERKKREEEKAKLRKVKEKEELEKGRKEQSKQREPQKRPD----EEVLVLRGT 361

Query: 421  PELVAPPEKEAEEESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEP 480
            P+  A  E++ +  +T        KKKK+K+K+K             EK ++ K    E 
Sbjct: 362  PDAGAASEEKGDIAATLEDDNEGDKKKKDKKKKK------------TEKDDKEK----EK 421

Query: 481  KKGAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQA 540
            KKG +KS          V+ +QEA+A+ +EEEER+KREEEER+++ EE   + +E ER  
Sbjct: 422  KKGPSKST---------VKAIQEALAKLREEEERQKREEEERIKRLEELEAKRKEEERLE 481

Query: 541  EEAKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAP 600
            +E + RKK++EKE+  R K EGKLLT  Q+E + R E     + +    +P   S  S P
Sbjct: 482  QEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEVTLRHLQAQGVEVP---SKDSLP 541

Query: 601  AKRPKYQTKKTKPAHHQTNGNAQTKA------MEHIEEKIQEK-------DVAETEILES 660
             KRP Y+ KK K    Q       +       +E +++ + EK       D  E E  E 
Sbjct: 542  KKRPVYEDKKKKKTPQQLESKEALETVEVSAPVEVVDQGVPEKEETPPSVDAEEDEETED 601

Query: 661  EKIEAVELMHVEEKSD--------VIEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSS 720
              ++  E M  +E+ +         +E   + E +E+EDEDE D++  +D          
Sbjct: 602  AGLDDWEAMASDEEREKEGNMIHIEVEENPEEEEEEEEDEDEEDSEDEEDEGDSEGSDGD 661

Query: 721  FADEELESEPENGMKKDTKNGAGAKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGK 780
              D +L  E + G   DTK         P +         S D   K    E    DK K
Sbjct: 662  EEDYKLSDEKDLGKAGDTK---------PNKDASSDSEYDSDDDRTK----EERAYDKAK 721

Query: 781  RKDDAVKKKASIPDATPVPQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGIT 840
            R+ +    K  +     V   E LR+PI C++GHVDTGKTK+LD +R T+VQ+GEAGGIT
Sbjct: 722  RRIE----KRRLEHGKNV-NTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGIT 781

Query: 841  QQIGATYFPAENIRERTRELK--ADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAIL 900
            QQIGAT  P E I E+T+ +K      +++PG+LIIDTPGHESF+NLR+RGS LCD+AIL
Sbjct: 782  QQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAIL 841

Query: 901  VVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQN 960
            VVDIMHGLEPQTIES+N+L+ +   FIVALNK+DRLY WK   ++ +  T+K+Q K+ ++
Sbjct: 842  VVDIMHGLEPQTIESINILKSKKCPFIVALNKIDRLYDWKKSPDSDVAVTLKKQKKNTKD 901

Query: 961  EFNMR----LIQKKFKGDHILIVHQ----------------------------------- 1020
            EF  R    +++   +G +  + ++                                   
Sbjct: 902  EFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTM 961

Query: 1021 -----------QCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALL 1080
                       +  V+EVK + G GTTIDVIL+NG L EGD I+     GPIVT IR LL
Sbjct: 962  LSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLL 1021

Query: 1081 TPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAME 1140
             P PMKELRVK  Y  HKE++AAQG+KI G+ LE  +AG  L V   +D++  +KD  + 
Sbjct: 1022 LPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKDDEIPVLKDELIH 1081

Query: 1141 DMKSVLSRID-----------------------KTDDT---------------------- 1164
            ++K  L+ I                        KT +                       
Sbjct: 1082 ELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVML 1141

BLAST of HG10022581 vs. ExPASy TrEMBL
Match: A0A5D3BXH6 (Eukaryotic translation initiation factor 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold675G00600 PE=4 SV=1)

HSP 1 Score: 1831.6 bits (4743), Expect = 0.0e+00
Identity = 1076/1342 (80.18%), Postives = 1117/1342 (83.23%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKK+ STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KKKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAPPEKEAE
Sbjct: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPPEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQEKDVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKGKRK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1163
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. ExPASy TrEMBL
Match: A0A5A7U6Q9 (Eukaryotic translation initiation factor 5B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2606G00100 PE=4 SV=1)

HSP 1 Score: 1831.6 bits (4743), Expect = 0.0e+00
Identity = 1076/1342 (80.18%), Postives = 1117/1342 (83.23%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKK+ STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKNNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KKKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAPPEKEAE
Sbjct: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPPEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQEKDVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQEKDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKGKRK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGKRKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1163
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. ExPASy TrEMBL
Match: A0A1S3CFD4 (Eukaryotic translation initiation factor 5B OS=Cucumis melo OX=3656 GN=LOC103500089 PE=4 SV=1)

HSP 1 Score: 1827.0 bits (4731), Expect = 0.0e+00
Identity = 1074/1343 (79.97%), Postives = 1116/1343 (83.10%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDD+DD DGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD
Sbjct: 61   GKKGNSKASQLKE-DDDEDDGDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120

Query: 121  GADDDDDSVLTAEK-DDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVI 180
            GADDD++SVLTAEK DDDEEE  AIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED I
Sbjct: 121  GADDDEESVLTAEKDDDDEEEHPAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDAI 180

Query: 181  ENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTS 240
            +NEIRVDEDI DEPV+ FTGKKKSSKGGKKA SAF+GFSGLDYED+DRDD KD +EDV S
Sbjct: 181  DNEIRVDEDI-DEPVVEFTGKKKSSKGGKKALSAFSGFSGLDYEDDDRDDQKD-EEDVAS 240

Query: 241  ISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGK 300
            ISFSGKKKKSAK SKKSGNLF+AALADEEND DVS+SE NKLD+DGV+EDDVNVIAFSGK
Sbjct: 241  ISFSGKKKKSAKASKKSGNLFSAALADEENDGDVSMSETNKLDHDGVDEDDVNVIAFSGK 300

Query: 301  KKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKS 360
            KKSSKKKS STFTA+ DENAQGNE KDVVVPEI NTVSSNLDSDLSNANKTE +AETSK+
Sbjct: 301  KKSSKKKSNSTFTALSDENAQGNEAKDVVVPEIHNTVSSNLDSDLSNANKTEAMAETSKN 360

Query: 361  KKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAE 420
            KK KKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL SQEA+VENPPELVAP EKEAE
Sbjct: 361  KKXKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLLSQEAKVENPPELVAPAEKEAE 420

Query: 421  EESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480
            E+STESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK
Sbjct: 421  EDSTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEK 480

Query: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREK 540
            KVPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREK
Sbjct: 481  KVPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREK 540

Query: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTK 600
            EKLLRKKLEGKLLTGKQKEEQRRLEAMR QILS+ GGLPLSTSDPSAPAKRPKYQTKKTK
Sbjct: 541  EKLLRKKLEGKLLTGKQKEEQRRLEAMRKQILSNTGGLPLSTSDPSAPAKRPKYQTKKTK 600

Query: 601  PAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNE 660
            P+HHQTNGNAQTKA+EHIEEKIQE+DVAETE+LESEKIEAVELMHVEEKS ++EATEDNE
Sbjct: 601  PSHHQTNGNAQTKAVEHIEEKIQERDVAETEVLESEKIEAVELMHVEEKSGILEATEDNE 660

Query: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKG 720
            IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAPAQKG
Sbjct: 661  IQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPAQKG 720

Query: 721  LPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMG 780
            LPSQ IKSQDIENKKKQ EVEV DKG+RK+DAV+KKASIPDATPV QEENLRSPICCIMG
Sbjct: 721  LPSQSIKSQDIENKKKQDEVEVPDKGERKEDAVRKKASIPDATPVQQEENLRSPICCIMG 780

Query: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840
            HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI
Sbjct: 781  HVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLI 840

Query: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900
            IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR
Sbjct: 841  IDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDR 900

Query: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK--------------------- 960
            LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                     
Sbjct: 901  LYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSI 960

Query: 961  -----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNG 1020
                        D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNG
Sbjct: 961  VPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNG 1020

Query: 1021 VLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080
            VLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH
Sbjct: 1021 VLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEH 1080

Query: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT----------------------- 1140
            AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                       
Sbjct: 1081 AIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKS 1140

Query: 1141 ---------------------------------------------------DDTYKKVSI 1164
                                                               D+   K+ I
Sbjct: 1141 PAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFI 1200

BLAST of HG10022581 vs. ExPASy TrEMBL
Match: A0A0A0K6L4 (Eukaryotic translation initiation factor 5B OS=Cucumis sativus OX=3659 GN=Csa_7G325160 PE=4 SV=1)

HSP 1 Score: 1823.5 bits (4722), Expect = 0.0e+00
Identity = 1068/1342 (79.58%), Postives = 1113/1342 (82.94%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDDSAPAAAHGGGKSKKKTF+VDDDEYSIGTELSEEAQ++EEKVVITGKKK
Sbjct: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFAVDDDEYSIGTELSEEAQIQEEKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+K+ DDD+DDVDGVSEIVITGKKKGKSKKGGSSSAF++SSFGLLEEEG+D
Sbjct: 61   GKKGNSKASQLKD-DDDEDDVDGVSEIVITGKKKGKSKKGGSSSAFSSSSFGLLEEEGID 120

Query: 121  GADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDVIE 180
             ADDD++SVLT EKDDDEEE SAIKFSGKKKSSKSSKKSGFSAVSAFTALDD+NDED I+
Sbjct: 121  DADDDEESVLTTEKDDDEEEHSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDDNDEDAID 180

Query: 181  NEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVTSI 240
            NEIR DEDID EPVI FTGKKKSSKGGKKAGSAF+GFSGLDYEDEDRDD KD +EDVTSI
Sbjct: 181  NEIRADEDIDGEPVIEFTGKKKSSKGGKKAGSAFSGFSGLDYEDEDRDDKKD-EEDVTSI 240

Query: 241  SFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSGKK 300
            SFSGKKKKSAK SKKSGN F+AALADEEND D S+SE NKLD+DGVNEDD+NVIAFSGKK
Sbjct: 241  SFSGKKKKSAKASKKSGNSFSAALADEENDGDFSMSETNKLDHDGVNEDDLNVIAFSGKK 300

Query: 301  KSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSKSK 360
            KSSKKKS ST TA+ DENAQ NE KDVVVPEI NTVSSNLDSDLSNANKTE VAETSK+K
Sbjct: 301  KSSKKKSNSTVTALSDENAQANEAKDVVVPEIHNTVSSNLDSDLSNANKTEAVAETSKNK 360

Query: 361  KKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEAEE 420
            KKKKKSGRTAQEEDDLDKILAELGEGP +SKPADPPL  QEA+VENPPELVAPPEKEAEE
Sbjct: 361  KKKKKSGRTAQEEDDLDKILAELGEGPAISKPADPPLFFQEAKVENPPELVAPPEKEAEE 420

Query: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEKK 480
            ESTESAAARKKKKKKEKEKEKKAAAAAAAAEG+DEK+EEVK+EIIEPKKGAAKSKVPEKK
Sbjct: 421  ESTESAAARKKKKKKEKEKEKKAAAAAAAAEGSDEKVEEVKSEIIEPKKGAAKSKVPEKK 480

Query: 481  VPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKKEREKE 540
            VPKHVREMQEAMARRKEEEERRKREEEERL+KEEEER RLEELERQAEEAKRRKKEREKE
Sbjct: 481  VPKHVREMQEAMARRKEEEERRKREEEERLKKEEEERLRLEELERQAEEAKRRKKEREKE 540

Query: 541  KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQTKKTKP 600
            KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILS+AGGLPLSTSDPSAPAKRPKYQTKKTKP
Sbjct: 541  KLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQTKKTKP 600

Query: 601  AHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNEI 660
            +HHQTNGNAQTK +EHI EKIQEKDVAETE+LESEKIEAVELMHVEEKS V+EATEDNEI
Sbjct: 601  SHHQTNGNAQTKVVEHIVEKIQEKDVAETEVLESEKIEAVELMHVEEKSGVLEATEDNEI 660

Query: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGAKLAAPAQKGL 720
            QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPEN MKKD KNGAGAKLAAP+QKGL
Sbjct: 661  QEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENDMKKDRKNGAGAKLAAPSQKGL 720

Query: 721  PSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMGH 780
            PSQ IKSQDIENKKKQ  VEVADKGKRK+DAV+KKASI DATPV QEENLRSPICCIMGH
Sbjct: 721  PSQSIKSQDIENKKKQDGVEVADKGKRKEDAVRKKASISDATPVQQEENLRSPICCIMGH 780

Query: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840
            VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII
Sbjct: 781  VDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLII 840

Query: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900
            DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL
Sbjct: 841  DTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRL 900

Query: 901  YGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK---------------------- 960
            YGWK+IRNAPILKTMKQQTKDVQNEFNMRLIQ   +FK                      
Sbjct: 901  YGWKSIRNAPILKTMKQQTKDVQNEFNMRLIQIITQFKEQGLNTELYYKNKEMGETFSIV 960

Query: 961  ----------GDHILIVHQ----------------QCTVLEVKVVEGHGTTIDVILVNGV 1020
                       D +L++ Q                QCTVLEVKVVEGHGTTIDVILVNGV
Sbjct: 961  PTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHGTTIDVILVNGV 1020

Query: 1021 LHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080
            LHEGDQIV     GPIVT+IRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA
Sbjct: 1021 LHEGDQIVVCGMQGPIVTSIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHA 1080

Query: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT------------------------ 1140
            IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT                        
Sbjct: 1081 IAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTGEGVCVQASTLGSLEALLEFLKSP 1140

Query: 1141 --------------------------------------------------DDTYKKVSID 1164
                                                              D+   K+ I 
Sbjct: 1141 AVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEARELADELGVKIFIA 1200

BLAST of HG10022581 vs. ExPASy TrEMBL
Match: A0A6J1F778 (Eukaryotic translation initiation factor 5B OS=Cucurbita moschata OX=3662 GN=LOC111442774 PE=4 SV=1)

HSP 1 Score: 1731.5 bits (4483), Expect = 0.0e+00
Identity = 1041/1353 (76.94%), Postives = 1090/1353 (80.56%), Query Frame = 0

Query: 1    MGRKKPTARDDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGKKK 60
            MGRKKPTARDDD APAAA GGGKSKKKTF+VDDDEYSIGTEL+EEAQ +E KVVITGKKK
Sbjct: 1    MGRKKPTARDDDGAPAAAQGGGKSKKKTFAVDDDEYSIGTELTEEAQDQEVKVVITGKKK 60

Query: 61   GKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGMD 120
            GKKGNSKASQ+KE DDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEG D
Sbjct: 61   GKKGNSKASQLKE-DDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEGKD 120

Query: 121  GA---DDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDED 180
            GA   DDDD+SVLTAEKDDDEEEDS IKFSGKKKSSKSSKKSGFSAVSAF ALDDE DED
Sbjct: 121  GANDDDDDDESVLTAEKDDDEEEDSVIKFSGKKKSSKSSKKSGFSAVSAFGALDDEKDED 180

Query: 181  VIENEIRVDEDIDDEPVIAFTG-KKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDED 240
            VI++E   +E+IDDEPVI+FTG KKKSS+GGKKAG+AFTGFSGLDYEDEDR+DD D  ED
Sbjct: 181  VIDDE---NEEIDDEPVISFTGKKKKSSQGGKKAGNAFTGFSGLDYEDEDRNDDMDDGED 240

Query: 241  VTSISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAF 300
            VT+I+FSGKKKKS+K SKKSGNLF+AALADEEND D SIS+P+KLD DGV+EDDVNVI+F
Sbjct: 241  VTTITFSGKKKKSSKGSKKSGNLFSAALADEENDGDDSISKPDKLDIDGVDEDDVNVISF 300

Query: 301  SGKKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAET 360
            SGKKKSSKKKS+STFTA+ DENA G+        EILNT SSNLDSDLS A KTE VAET
Sbjct: 301  SGKKKSSKKKSSSTFTALSDENALGD--------EILNTASSNLDSDLSKA-KTEAVAET 360

Query: 361  SKSKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEK 420
            SK+KKKKKKSGRTAQEEDDLDKILAELGEGP  SKPADPPLSSQEA+VEN PE+VAPPEK
Sbjct: 361  SKNKKKKKKSGRTAQEEDDLDKILAELGEGPATSKPADPPLSSQEAKVENQPEVVAPPEK 420

Query: 421  EAEEESTESAAARKKKKKKEKEKEKK-AAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKSK 480
            E EEESTESAAARKKKKKKEKEKEKK AAAAAAAAEGNDEKIEEVKTEIIEPKKGAAK K
Sbjct: 421  EVEEESTESAAARKKKKKKEKEKEKKAAAAAAAAAEGNDEKIEEVKTEIIEPKKGAAKGK 480

Query: 481  VPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKRRKK 540
            V EKKVPKHVREMQEAMARRKEEEERRKREEEE+LRKEEEERRR EELERQAEEAKRRKK
Sbjct: 481  VVEKKVPKHVREMQEAMARRKEEEERRKREEEEKLRKEEEERRRQEELERQAEEAKRRKK 540

Query: 541  EREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSAPAKRPKYQT 600
            EREKEKLLRKK EGKLLTGKQKEEQRRLEAMRNQILS+AGGLPLSTSDPSAPAKRPKYQT
Sbjct: 541  EREKEKLLRKKQEGKLLTGKQKEEQRRLEAMRNQILSNAGGLPLSTSDPSAPAKRPKYQT 600

Query: 601  KKTKPAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEAT 660
            KKTKPAHHQTNG+AQTK +EH+EE+IQEKD+AETEILESEKIEAVE    EEKSD IEAT
Sbjct: 601  KKTKPAHHQTNGSAQTKVVEHVEERIQEKDLAETEILESEKIEAVESTLEEEKSDAIEAT 660

Query: 661  EDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKN------GAG 720
             DNEIQEDED+DEWDAKSWDDAVVDLSLKSSFADEELESE ENGMKKD KN      GA 
Sbjct: 661  VDNEIQEDEDDDEWDAKSWDDAVVDLSLKSSFADEELESESENGMKKDRKNSAPTSGGAV 720

Query: 721  AKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEEN 780
            AK AAPAQK LPSQPIKSQDIEN KKQHEVEV +KGK KDDAVKKK SIPDATP  QEEN
Sbjct: 721  AKPAAPAQKSLPSQPIKSQDIENTKKQHEVEVVEKGKGKDDAVKKKTSIPDATPKQQEEN 780

Query: 781  LRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKAD 840
            LRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKAD
Sbjct: 781  LRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKAD 840

Query: 841  AKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTE 900
            AKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTE
Sbjct: 841  AKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTE 900

Query: 901  FIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRLIQ--KKFK----------- 960
            FI+ALNKVDRLYGWKTIRNAPILKTMKQQ+KDVQNEFNMRLIQ   +FK           
Sbjct: 901  FIIALNKVDRLYGWKTIRNAPILKTMKQQSKDVQNEFNMRLIQIITQFKEQGLNTELYYK 960

Query: 961  ---------------------GDHILIVHQ----------------QCTVLEVKVVEGHG 1020
                                  D +L++ Q                QCTVLEVKVVEGHG
Sbjct: 961  NKEMGETFSIVPTSAVTGEGIPDMLLLLVQWAQKTMTKKLTYSDEVQCTVLEVKVVEGHG 1020

Query: 1021 TTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQG 1080
            TTIDVILVNGVLHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQG
Sbjct: 1021 TTIDVILVNGVLHEGDQIVVCGMQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQG 1080

Query: 1081 IKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKT------------- 1140
            IKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRID++             
Sbjct: 1081 IKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDRSGEGVCVQASTLGS 1140

Query: 1141 ------------------------------------------------------------ 1164
                                                                        
Sbjct: 1141 LEALLEFLKSPAVSIPVSGISIGPVHKKDVMKASVMLEKKKEYATILAFDVKVTPEAREL 1200

BLAST of HG10022581 vs. TAIR 10
Match: AT1G76810.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 981.5 bits (2536), Expect = 6.2e-286
Identity = 700/1376 (50.87%), Postives = 834/1376 (60.61%), Query Frame = 0

Query: 1    MGRKKPTAR--DDDSAPAAAH--GGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEK-VVI 60
            MGRKKP+AR  D +  P A+   G  KSKKK   +DDDEYSIGTELSEE++VEEEK VVI
Sbjct: 1    MGRKKPSARGGDAEQQPPASSLVGATKSKKKGAQIDDDEYSIGTELSEESKVEEEKVVVI 60

Query: 61   TGKKKGKKGNSKASQIKENDDDDDDV------DGVSEIVITGKKKGKSKKGGSSSAFTAS 120
            TGKKKGKKGN K +Q  ++DD  D V      D V EI   GKKK K KKGG S      
Sbjct: 61   TGKKKGKKGNKKGTQQDDDDDFSDKVSAAGVKDDVPEIAFVGKKKSKGKKGGGS-----V 120

Query: 121  SFGLLEEEGMDGADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTA 180
            SF LL         DD+D     EK+D+E                               
Sbjct: 121  SFALL---------DDED-----EKEDNES------------------------------ 180

Query: 181  LDDENDEDVIENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAF--TGFSGLDYEDEDR 240
                            D D DDEPVI+FTGKK +SK GKK G++F  + F  L  +D+D 
Sbjct: 181  ----------------DGDKDDEPVISFTGKKHASKKGKKGGNSFAASAFDALGSDDDDT 240

Query: 241  DDDKDGDEDVTSISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVN 300
            ++  + +E+ + I+FSGKKKKS+K+SKK+ N FTA L DEE   D S S     D + + 
Sbjct: 241  EEVHEDEEEESPITFSGKKKKSSKSSKKNTNSFTADLLDEEEGTDASNSRD---DENTIE 300

Query: 301  EDDVNVIAFSGKKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNA 360
            +++   + FSGKKKSSKKK  S   ++ D+                    S  D   ++ 
Sbjct: 301  DEESPEVTFSGKKKSSKKKGGSVLASVGDD--------------------SVADETKTSD 360

Query: 361  NKTEGVAETSKSKKKKK--KSGRTAQEEDDLDKILAELGEGPTVSKPA-DPPLSSQEAQV 420
             K   V ET KSKKKKK  KSGRT QEE+DLDK+LA LGE P   +PA   P+  + AQ 
Sbjct: 361  TKNVEVVETGKSKKKKKNNKSGRTVQEEEDLDKLLAALGETPAAERPASSTPVEEKAAQ- 420

Query: 421  ENPPELVAPPEKEAEEE-STESAAARKKKKKKEKEKEKKAAAAAAA---AEGNDEKIEEV 480
               PE VAP E   E+E   E+AAA+KKKKKKEKEKEKKAAAAAAA    E  +EK EE 
Sbjct: 421  ---PEPVAPVENAGEKEGEEETAAAKKKKKKKEKEKEKKAAAAAAATSSVEVKEEKQEES 480

Query: 481  KTEIIEPKKGAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRL 540
             TE ++PKK  AK K  EKK+PKHVREMQEA+ARR+E EER+K+EEEE+LRKEEEERRR 
Sbjct: 481  VTEPLQPKKKDAKGKAAEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQ 540

Query: 541  EELERQAEEAKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLS 600
            EELE QAEEAKR++KE+EKEKLLRKKLEGKLLT KQK E ++ EA +NQ+L++ GGLP++
Sbjct: 541  EELEAQAEEAKRKRKEKEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAGGGLPVA 600

Query: 601  TSDPSA-PAKRPKYQTKKTKPAHHQTNGNAQTK-AMEHIEEKIQEKD-VAETEILESEKI 660
             +D  A  +KRP Y  KK        + + Q +  +E  E +  E+D + E  + ++ K+
Sbjct: 601  DNDGDATSSKRPIYANKKKSSRQKGIDTSVQGEDEVEPKENQADEQDTLGEVGLTDTGKV 660

Query: 661  EAVELMHVEEKSDVIEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEP- 720
            + +EL++ +E S   +  ++N ++ED++EDEWDAKSW    VDL+LK  F DEE E++P 
Sbjct: 661  DLIELVNTDENSGPADVAQENGVEEDDEEDEWDAKSW--GTVDLNLKGDFDDEEEEAQPV 720

Query: 721  -----ENGMKK--DTKNGAGAKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGK--- 780
                 ++ + K  D++  A    A PA  G P        I   K   EVE A + K   
Sbjct: 721  VKKELKDAISKAHDSEPEAEKPTAKPAGTGKPL-------IAAVKATPEVEDATRTKRAT 780

Query: 781  RKDDAVKKKASIPDATPVPQEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGIT 840
            R  DA KK   +  +  +  EENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGIT
Sbjct: 781  RAKDASKKGKGLAPSESIEGEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGIT 840

Query: 841  QQIGATYFPAENIRERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVV 900
            QQIGATYFPAENIRERT+ELKADAKLKVPGLL+IDTPGHESFTNLRSRGS LCDLAILVV
Sbjct: 841  QQIGATYFPAENIRERTKELKADAKLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVV 900

Query: 901  DIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEF 960
            DIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKT +NAPI+K MKQQ KDV NEF
Sbjct: 901  DIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEF 960

Query: 961  NMRL-----------------IQKKFKGDHILIV-------------------------- 1020
            N+RL                  + K  GD   IV                          
Sbjct: 961  NLRLKNIINEFQEQGLNTELYYKNKDMGDTFSIVPTSAISGEGVPDLLLWLVQWAQKTMV 1020

Query: 1021 -------HQQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTP 1080
                     QCTVLEVKV+EGHGTTIDV+LVNG LHEGDQIV     GPIVTTIRALLTP
Sbjct: 1021 EKLTYVDEVQCTVLEVKVIEGHGTTIDVVLVNGELHEGDQIVVCGLQGPIVTTIRALLTP 1080

Query: 1081 HPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDM 1140
            HPMKELRVKGTYLH+KEIKAAQGIKIT QGLEHAIAGT+LHVVGP+DD+E IK+SAMEDM
Sbjct: 1081 HPMKELRVKGTYLHYKEIKAAQGIKITAQGLEHAIAGTALHVVGPDDDIEAIKESAMEDM 1140

Query: 1141 KSVLSRIDKT-------------------------------------------------- 1164
            +SVLSRIDK+                                                  
Sbjct: 1141 ESVLSRIDKSGEGVYVQASTLGSLEALLEYLKSPAVKIPVSGIGIGPVHKKDVMKAGVML 1200

BLAST of HG10022581 vs. TAIR 10
Match: AT1G76720.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 1.1e-234
Identity = 618/1344 (45.98%), Postives = 757/1344 (56.32%), Query Frame = 0

Query: 1    MGRKKPTAR---DDDSAPAAAH-GGGKSKKKTFSVD-DDEYSIGTELSEEAQVEEEKVVI 60
            MGRKKP++    DD+  PAA+   G +SKKK    D +D+YSI T        EEEKVVI
Sbjct: 1    MGRKKPSSSRGGDDEQPPAASSLVGAESKKKAVQSDEEDKYSINT--------EEEKVVI 60

Query: 61   TGKKKGKKGNSKASQIKENDDDDDDVDGVSEIVITG-KKKGKSKKGGSSSAFTASSFGLL 120
            TGK   KK N K +Q  ++DDDDD  + V E    G KKK K K  G S +F        
Sbjct: 61   TGK---KKSNKKVTQ--KHDDDDDFTEAVPENGFVGKKKKSKGKNRGGSVSF-------- 120

Query: 121  EEEGMDGADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDEN 180
                         ++L+ +++ D+ E +                                
Sbjct: 121  -------------ALLSGKEETDDNESNG------------------------------- 180

Query: 181  DEDVIENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGD 240
                         D DDEPVI+FTGKK +S  GKK G A + F  L  + +D + D D +
Sbjct: 181  -------------DKDDEPVISFTGKKNASNKGKK-GFAVSAFDALGGDKDDEEVDGD-E 240

Query: 241  EDVTSISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVI 300
            E V+ I+FS                    + DEE+ +                      I
Sbjct: 241  EQVSPITFS-----------------EGTMGDEESLE----------------------I 300

Query: 301  AFSGKKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVA 360
             FSGKKK S   ++    ++ DE +Q  +  D    E++ T                   
Sbjct: 301  TFSGKKKGSIVLASLGDDSVADETSQA-KTPDTKSVEVIET------------------G 360

Query: 361  ETSKSKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAP- 420
            +  K KK K K  RT +EEDDLDK+LAELGE P   +PA      ++ Q +  P  VAP 
Sbjct: 361  KIKKKKKNKNKVARTLEEEDDLDKLLAELGETPAAERPASSTPEVEKVQAQPGP--VAPV 420

Query: 421  ---PEKEAEEESTESAAARKKKKKKEKEKEKKAAAAA-AAAEGNDEKIEEVKTEIIEPKK 480
                EKE E+E+ E+AAA+KKKKKKEK+KEKKAAAAA ++ E  +EK EE  TE ++PKK
Sbjct: 421  ENAGEKEGEKETVETAAAKKKKKKKEKDKEKKAAAAATSSVEAKEEKQEESVTEPLQPKK 480

Query: 481  GAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEE 540
              AK K  EKK+PKHVREMQEA+ARR+E EER+K+EEEE+LRKEEEERRR EELE QAEE
Sbjct: 481  KDAKGKAAEKKIPKHVREMQEALARRQEAEERKKKEEEEKLRKEEEERRRQEELEAQAEE 540

Query: 541  AKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTS-DPSAPA 600
            AKR++KE+EKEKLLRKKLEGKLLT KQK E ++ EA +NQ+L++  GLP++     +  +
Sbjct: 541  AKRKRKEKEKEKLLRKKLEGKLLTAKQKTEAQKREAFKNQLLAAGRGLPVADDVGDATSS 600

Query: 601  KRPKYQTKKTKPAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEK 660
            KRP Y  KK KP+  + N  +     E   ++     + E    ++EK++ +E  +  EK
Sbjct: 601  KRPIYANKK-KPSRQKGNDTSVQVEDEVEPQENHAATLGEVGSEDTEKVDLLESANTGEK 660

Query: 661  SDVIEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTK-NG 720
            S   +  ++N ++ED   DEWDAKSWD+  VDL +     D+E E++P   +KK+ K + 
Sbjct: 661  SGPADVAQENGVEED---DEWDAKSWDN--VDLKID----DKEEEAQPV--VKKELKAHD 720

Query: 721  AGAKLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQE 780
            +  +   P  K     P     +     +   EV D   +   A K K   P        
Sbjct: 721  SDHETEKPTAK-----PAGMSKLTTGAVKAISEVEDAATQTKRAKKGKCLAPSEFIKEGG 780

Query: 781  ENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELK 840
            ENLRS ICCIMGHVD+GKTKLLDCIRGTNVQEGEAGGITQQIGATYFPA+NIRERTRELK
Sbjct: 781  ENLRSIICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAKNIRERTRELK 840

Query: 841  ADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRN 900
            ADAKLKVPGLL+IDTPGHESFTNLRSRGS LCDLAILVVDI HGLEPQTIESLNLLRMRN
Sbjct: 841  ADAKLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDIKHGLEPQTIESLNLLRMRN 900

Query: 901  TEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRL----------------- 960
            TEFI+ALNKVDRLYGWKT +NAPI+K MKQQ KDV NEFN+RL                 
Sbjct: 901  TEFIIALNKVDRLYGWKTCKNAPIVKAMKQQNKDVTNEFNIRLTDIITQFKEQGLNTEIY 960

Query: 961  IQKKFKGDHILIV---------------------------------HQQCTVLEVKVVEG 1020
             + K  G+   IV                                   QCTVLEVKV+EG
Sbjct: 961  YKNKEMGETFSIVPTSAKSGEGVPDLLLWLVQWAQKTMVEKLTYVDEVQCTVLEVKVIEG 1020

Query: 1021 HGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAA 1080
            HGTTIDV+LVNG LHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAA
Sbjct: 1021 HGTTIDVVLVNGELHEGDQIVVCGLQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAA 1080

Query: 1081 QGIKITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTDD--------- 1140
            QGIKIT QGLEHAIAGTSLHVVGP+DD+E +K+SAMEDM+SVLSRIDK+ +         
Sbjct: 1081 QGIKITAQGLEHAIAGTSLHVVGPDDDIEAMKESAMEDMESVLSRIDKSGEGVYVQTSTL 1140

Query: 1141 -----------------------------------------------TYKKVSIDE---- 1162
                                                           T  +   DE    
Sbjct: 1141 GSLEALLEFLKTPAVNIPVSEGYNEGWSDAREEKEYATILAFDVKVTTEARELADEMGVK 1186

BLAST of HG10022581 vs. TAIR 10
Match: AT1G76820.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 793.9 bits (2049), Expect = 1.8e-229
Identity = 602/1350 (44.59%), Postives = 737/1350 (54.59%), Query Frame = 0

Query: 1    MGRKKPTAR--DDDSAPAAAHGGGKSKKKTFSVDDDEYSIGTELSEEAQVEEEKVVITGK 60
            MGRK+P++R  DD+   A++  G KS KK+  +DDDEYSI     EE +VEE KVVITGK
Sbjct: 1    MGRKEPSSRGGDDEQPLASSLVGSKSDKKSVQIDDDEYSI---CLEEPKVEENKVVITGK 60

Query: 61   KKGKKGNSKASQIKENDDDDDDVDGVSEIVITGKKKGKSKKGGSSSAFTASSFGLLEEEG 120
            KKGKK N K +Q ++ DDDDD  D                                    
Sbjct: 61   KKGKKRNKKGTQ-QQQDDDDDFAD------------------------------------ 120

Query: 121  MDGADDDDDSVLTAEKDDDEEEDSAIKFSGKKKSSKSSKKSGFSAVSAFTALDDENDEDV 180
                                      KF+                               
Sbjct: 121  --------------------------KFA------------------------------- 180

Query: 181  IENEIRVDEDIDDEPVIAFTGKKKSSKGGKKAGSAFTGFSGLDYEDEDRDDDKDGDEDVT 240
            +E E+  D         AF G  K SKG K  G                           
Sbjct: 181  VEEEVVPDN--------AFVGNNKKSKGKKDGG--------------------------- 240

Query: 241  SISFSGKKKKSAKTSKKSGNLFTAALADEENDDDVSISEPNKLDNDGVNEDDVNVIAFSG 300
            S+SF                   A LADE+N ++         DND         I FSG
Sbjct: 241  SVSF-------------------ALLADEDNREEAE-------DND---------ITFSG 300

Query: 301  KKKSSKKKSTSTFTAIRDENAQGNEVKDVVVPEILNTVSSNLDSDLSNANKTEGVAETSK 360
            +KKSSKKKS+S   +I DE +  ++  D    E++ +                     SK
Sbjct: 301  RKKSSKKKSSSVLASIGDEESSQSKTSDSKSVELVESA-------------------RSK 360

Query: 361  SKKKKKKSGRTAQEEDDLDKILAELGEGPTVSKPADPPLSSQEAQVENPPELVAPPEKEA 420
              KKK KSGRT QE+DDLDK+LAELGE P   KPA     S+E + +  PE VAP E   
Sbjct: 361  KHKKKNKSGRTLQEDDDLDKLLAELGETPAAGKPA-----SEEEKDQAQPEPVAPVENTG 420

Query: 421  EEES---TESAAARKKKKKKEKEKEKKAAAAAAA---AEGNDEKIEEVKTEIIEPKKGAA 480
            E+E          RK+K+++ K+++KKAAAAAAA    E  +EK EE  TE ++P+K  A
Sbjct: 421  EKEKRRLLRLLQPRKRKRRRRKKRKKKAAAAAAATSSVEAKEEKQEESVTEPLQPRKKDA 480

Query: 481  KSKVPEKKVPKHVREMQEAMARRKEEEERRKREEEERLRKEEEERRRLEELERQAEEAKR 540
            K K  EKK+PKHVRE+QEA+ARR+E +ER+K+EEEE+LRKEEEERRR EEL+ QAEEAKR
Sbjct: 481  KGKAAEKKIPKHVREIQEALARRQEAKERKKKEEEEKLRKEEEERRRQEELDAQAEEAKR 540

Query: 541  RKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEAMRNQILSSAGGLPLSTSDPSA-PAKRP 600
            ++KE+EKEKLLRKKLEGKLLT KQK E ++ EA +NQ+L++ GGLP++  D  A  +KRP
Sbjct: 541  KRKEKEKEKLLRKKLEGKLLTAKQKSEAQKREAFKNQLLAAGGGLPVADDDDDATSSKRP 600

Query: 601  KYQTKKTKPAHHQTNGNAQTKAMEHIEEKIQEKDVAETEILESEKIEAVELMHVEEKS-D 660
             Y  KK      +  GN  +  +E   E  +   + E    ++EK++ +E  ++ EKS  
Sbjct: 601  IYANKKKS---SRQKGNDTSVQVEGEVEPKENHALGEVGSADTEKVDLLESANLGEKSGG 660

Query: 661  VIEATEDNEIQEDEDEDEWDAKSWDDAVVDLSLKSSFADEELESEPENGMKKDTKNGAGA 720
              +  ++N ++ED   DEWDAKSWD+  VDL ++  F D+  E E ++ +KK+ K    A
Sbjct: 661  PADVAQENGVEED---DEWDAKSWDN--VDLKIRGDFDDK--EEEAQHVVKKEFK----A 720

Query: 721  KLAAPAQKGLPSQPIKSQDIENKKKQHEVEVADKGKRKDDAVKKKASIPDATPVPQEENL 780
              +    +   ++P     +E    +   EV D   +   A K K   P+       E L
Sbjct: 721  HYSDHETEKPTAKPAGMSKLETAAVKAISEVEDAATQTKRAKKGKCLAPNEFIEEGGEKL 780

Query: 781  RSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRELKADA 840
            RS ICCIMGHVD+GKTKLLDCIRGTNVQEGEAGGITQQIGATYFPA+NIRERTRELKADA
Sbjct: 781  RSIICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAKNIRERTRELKADA 840

Query: 841  KLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRMRNTEF 900
            KLKVPGLL+IDTPGHESFTNLRSRGS LCDLAILVVDI HGL+PQTIESLNLLRMRNTEF
Sbjct: 841  KLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDITHGLQPQTIESLNLLRMRNTEF 900

Query: 901  IVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRL-----------------IQK 960
            I+ALNKVDRLYGWKT +NAPI+K MKQQ KDV NEFN+RL                  + 
Sbjct: 901  IIALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLRLKKIINEFQEQGLNTELYYKN 960

Query: 961  KFKGDHILIV---------------------------------HQQCTVLEVKVVEGHGT 1020
            K  G+   IV                                   QCTVLEVKV+EGHGT
Sbjct: 961  KDMGETFSIVPTSAISGEGVPDLLLWLVQWAQKTMVEKLTYVDEVQCTVLEVKVIEGHGT 1020

Query: 1021 TIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGI 1080
            TIDV+LVNG LHEGDQIV     GPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGI
Sbjct: 1021 TIDVVLVNGELHEGDQIVVCGLQGPIVTTIRALLTPHPMKELRVKGTYLHHKEIKAAQGI 1080

Query: 1081 KITGQGLEHAIAGTSLHVVGPEDDLEDIKDSAMEDMKSVLSRIDKTDD------------ 1140
            KIT QGLEHAIAGTSLHVVGP+DD+E +K+SAMEDM+SVLSRIDK+ +            
Sbjct: 1081 KITAQGLEHAIAGTSLHVVGPDDDIEAMKESAMEDMESVLSRIDKSGEGVYVQTSTLGSL 1140

Query: 1141 -----------------------TYKK--------------------------------- 1162
                                    +KK                                 
Sbjct: 1141 EALLEFLKTPAVNIPVSGIGIGPVHKKDIMKAGVMLEKKKEYATILAFDVKVTTEARELA 1144

BLAST of HG10022581 vs. TAIR 10
Match: AT1G21160.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 589.0 bits (1517), Expect = 9.0e-168
Identity = 461/1089 (42.33%), Postives = 602/1089 (55.28%), Query Frame = 0

Query: 270  DDDVSISEPNKLDNDGVNEDDVNVIAFSGKKKSSK-KKSTSTFTAIRDENAQGNEVKDVV 329
            DDD SI    KL      E+    I FS +KK  K KK  S   A+ D      E +D  
Sbjct: 34   DDDGSIG--TKLCEKPKAEE----IDFSARKKKLKSKKGGSVSFALLDVEDDKEEAEDG- 93

Query: 330  VPEILNTVSSNLDSDLSNANKTEGVAETSKSKKKKK-KSGR--TAQEEDDLDKILAELGE 389
                        D    +    E V ETS SKKKKK KSG+    +E+DD DKILA+ G 
Sbjct: 94   ------------DDKNPSIRSDEVVGETSMSKKKKKDKSGKHGVCEEDDDADKILAQHGI 153

Query: 390  GPTVSKPADPPLSSQEAQVENPPELVAPPEKEAEEESTESAAARKKKKKKEKEKEKKAAA 449
              TVS    P      AQ E         +K+ EE++ ESA A+KKKKKK+K+K+  A+ 
Sbjct: 154  TTTVS--TGPEAEIALAQPEQVASADGAVDKDGEEKTVESATAKKKKKKKDKDKKASASL 213

Query: 450  AAAAAEGNDEKIEEVKTEIIEPKKGAAKSKVPEKKVPKHVREMQEAMARRKEEEERRKRE 509
            A ++ E  +++            K   K KV EKKVPKHVRE QE +AR KE E+ +K+E
Sbjct: 214  AISSVEAKEDR----------QGKKDVKIKVAEKKVPKHVREKQETLARWKEAEDGKKKE 273

Query: 510  EEERLRKEEEERRRLEELERQAEEAKRRKKEREKEKLLRKKLEGKLLTGKQKEEQRRLEA 569
            EEERLRKEEEERR  EE ER+AEE ++++K R+ E    KK EG +LT KQK +  + EA
Sbjct: 274  EEERLRKEEEERRIEEEREREAEEIRQKRKIRKME----KKQEGLILTAKQKRDAAKNEA 333

Query: 570  MRNQILSSAGGLPLSTSDPSAPAKRPKYQTKK-------TKPAHHQTNGNAQTKAMEHIE 629
             R ++L+ AG L ++  +  + +KRP Y  K          PA  Q  G+ +TK     +
Sbjct: 334  FRKRVLTDAGSLLVADKNGDS-SKRPIYGNKNKLACKKANDPASVQAKGDGETKENHAAD 393

Query: 630  EKIQEKDVAETEILESEKIEAVELMHVEEKSDVIEATEDNEIQEDEDEDEWDAKSWDDAV 689
            E     D+     +E  ++  +E +  EE  + ++ +++N     ++ED WDAK+     
Sbjct: 394  EPCTLPDLVS---VEDRRVGIIESVDTEETHESVDVSQEN----GDEEDVWDAKT----- 453

Query: 690  VDLSLKSSFADEELESEPENGMKKDTKNGAG-AKLAAPAQKGLPSQPIKSQDIENKKKQH 749
             + ++K    DE  E +P+   KK  K+ A  A  + P       +P  S   +   K+ 
Sbjct: 454  -NFTIKGDSDDE--EEKPQPVFKKGLKDTASKAHDSVPGADRPTVKPGGSGKPKTAAKKA 513

Query: 750  EVEVADKGKRKDDAVKKKASIPDATPVPQEENLRSPICCIMGHVDTGKTKLLDCIRGTNV 809
              +V D  + KD + K +  + +      EENLRSPICCIMGHVD+GKTKLLDCIRGTNV
Sbjct: 514  MPKVDDTTRPKDTSKKDEGLVLNELAKEVEENLRSPICCIMGHVDSGKTKLLDCIRGTNV 573

Query: 810  QEGEAGGITQQIGATYFPAENIRERTRELKADAKLKVPGLLIIDTPGHESFTNLRSRGSG 869
            QEGEAGGITQQIGAT+FPAENIRERT+EL+A+AKLKVPG+L+IDTPGHESFTNLRSRGS 
Sbjct: 574  QEGEAGGITQQIGATFFPAENIRERTKELQANAKLKVPGILVIDTPGHESFTNLRSRGSN 633

Query: 870  LCDLAILVVDIMHGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTIRNAPILKTMKQ 929
            LCDLAILVVDIM GLEPQTIESLNLLR RN +FI+ALNKVDRLYGW+  +NAPI KTM Q
Sbjct: 634  LCDLAILVVDIMRGLEPQTIESLNLLRRRNVKFIIALNKVDRLYGWEKSKNAPIRKTMMQ 693

Query: 930  QTKDVQNEFNMRL--IQKKFK---------------GDHILIV----------------- 989
            QT DV  EF MRL  +Q +F+               G+ I I+                 
Sbjct: 694  QTGDVVKEFKMRLNRVQNQFQEQGLNSMLYYKNREMGETISILPASAISGEGIPDLLLFL 753

Query: 990  ----------------HQQCTVLEVKVVEGHGTTIDVILVNGVLHEGDQIV-----GPIV 1049
                              QCTVLEVKV+EGHG T+DV+LVNGVL EGDQIV     GPIV
Sbjct: 754  VQWAQKTMVEKLTYVDKVQCTVLEVKVIEGHGITVDVVLVNGVLREGDQIVVCGSQGPIV 813

Query: 1050 TTIRALLTPHPMKELRVKGTYLHHKEIKAAQGIKITGQGLEHAIAGTSLHVVGPEDDLED 1109
            TTIR+LLTP+PM E+RV GTY+ H+E+KAAQGIKI  QGLEHAIAGT+LHV+GP +D+E+
Sbjct: 814  TTIRSLLTPYPMNEMRVTGTYMPHREVKAAQGIKIAAQGLEHAIAGTALHVIGPNEDMEE 873

Query: 1110 IKDSAMEDMKSVLSRIDKT----------------------------------------- 1162
             K +AMED++SV++RIDK+                                         
Sbjct: 874  AKKNAMEDIESVMNRIDKSGEGVYVQASTLGSLEALLEFLKSSDVKIPVSGIGIGPVHKK 933

BLAST of HG10022581 vs. TAIR 10
Match: AT2G27700.2 (eukaryotic translation initiation factor 2 family protein / eIF-2 family protein )

HSP 1 Score: 398.3 bits (1022), Expect = 2.2e-110
Identity = 218/355 (61.41%), Postives = 251/355 (70.70%), Query Frame = 0

Query: 766  QEENLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAENIRERTRE 825
            +E+ +RSPICC+MGH+DTGKTKLLD I G NVQEGEAGG TQ++GATY  A NI E+T E
Sbjct: 30   EEDKVRSPICCMMGHIDTGKTKLLDYITG-NVQEGEAGGTTQKMGATYLSARNILEKTME 89

Query: 826  LKADAKLKVPGLLIIDTPGHESFTNLRSRGSGLCDLAILVVDIMHGLEPQTIESLNLLRM 885
            LK+D KLKVP  L IDTPG+E +TNLRSRG GLCD AILVVDIMHGLEPQTIE LNLLRM
Sbjct: 90   LKSDTKLKVPRPLFIDTPGYEFYTNLRSRGLGLCDFAILVVDIMHGLEPQTIECLNLLRM 149

Query: 886  RNTEFIVALNKVDRLYGWKTIRNAPILKTMKQQTKDVQNEFNMRL--IQKKFK------- 945
            +NTEFI+ALNKVDRLYGW+   NAPIL+ MKQQTKDV NEFNMRL  I  +FK       
Sbjct: 150  KNTEFIIALNKVDRLYGWRICENAPILEAMKQQTKDVINEFNMRLNEIINQFKEQGLNSE 209

Query: 946  --------GDHILIVH-----------------------------------QQCTVLEVK 1005
                    G+   IV                                    QQCTVLEVK
Sbjct: 210  LYYKNKEMGETFSIVPTCAISGEGIPDLLLLLVQLTQKTMVEKLITYVDKVQQCTVLEVK 269

Query: 1006 VVEGHGTTIDVILVNGVLHEGDQIV-----GPIVTTIRALLTPHPMKELRVKGTYLHHKE 1064
            V+EG+GTTIDV+LVNG LHEG QIV     GPIVTTIRALLTPHP+KEL V G ++HH+ 
Sbjct: 270  VMEGYGTTIDVVLVNGELHEGGQIVVCGLQGPIVTTIRALLTPHPIKELHVNGNHVHHEV 329