HG10022488 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022488
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	subtilisin-like protease SBT3.4
Location	Chr05: 24794711 .. 24795573 (+)
RNA-Seq Expression	HG10022488
Synteny	HG10022488

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAATACAAAGATGAGTTTTTCTTCATTCACCTTGTATCTTGTTTTATTGTTCACAATTTCTCTGAATTTGCTTCTTACTTCGGCGAGAATATCCGACAAATCCACCTCTAACATTGATGTACCATCATCATTGCATCTCGTTTACACCATCGAGAGGCCTAGCAATGAGAAACCTAAGGCATTTTACATCCAAATCCTAGCTTCTGTTCTTGGCAGGTATGTTTTTCATGAAAAATTATTCAAAGAAATTGCCACAATGTGTTGGACAATAATTATTCAATCAGATCACCACAATAATGTTCACAGTTTTGCACGGTTGGATGTAGATTCTAAAATTTGCAAGCTCCTAATAACCACGAGATACAAATTTGATAAGCTTTTAATGTTTGTCTCAAATGTCAAAATAGTTTAAGGCGCTCTCTTCAAATTAGTTCTCATCGCTCATCGTGGTTAATTGTTAGGAGCATCCACATCGAGACGATCTGTCATCGAATAATTTTTTGTTTTCTAAATATTCCTTTGCGTGAAATGATCCTGGGATAATTTAGGGTGAATTTTTGTGTCATTTTGTTGCTTTGTTGCAGTGAAGAAGCTGCTAAGAAAGCTCTTGCGTATAGTTTCAAGAGTTCCATGAGTGGCTTTGCAGCTAACCTGACTCCCGATCAAGTCAAGAAAATTTTGGGTAACTATCACTCTATGATTTTATTATCATTATTATTATTAACAACTTTAGAATATGATTAGATATTCCCTCACATTTTATATTTTGGGACTTTGGCAGCCCAACCAGGAGTTCTACATGTTGCACAAAGTGTTAACTATGATTTAACAGGAGGAAAAAGGAATAATGATGTATAG

mRNA sequence

ATGGCAAATACAAAGATGAGTTTTTCTTCATTCACCTTGTATCTTGTTTTATTGTTCACAATTTCTCTGAATTTGCTTCTTACTTCGGCGAGAATATCCGACAAATCCACCTCTAACATTGATGTACCATCATCATTGCATCTCGTTTACACCATCGAGAGGCCTAGCAATGAGAAACCTAAGGCATTTTACATCCAAATCCTAGCTTCTGTTCTTGGCAGTGAAGAAGCTGCTAAGAAAGCTCTTGCGTATAGTTTCAAGAGTTCCATGAGTGGCTTTGCAGCTAACCTGACTCCCGATCAAGTCAAGAAAATTTTGGCCCAACCAGGAGTTCTACATGTTGCACAAAGTGTTAACTATGATTTAACAGGAGGAAAAAGGAATAATGATGTATAG

Coding sequence (CDS)

ATGGCAAATACAAAGATGAGTTTTTCTTCATTCACCTTGTATCTTGTTTTATTGTTCACAATTTCTCTGAATTTGCTTCTTACTTCGGCGAGAATATCCGACAAATCCACCTCTAACATTGATGTACCATCATCATTGCATCTCGTTTACACCATCGAGAGGCCTAGCAATGAGAAACCTAAGGCATTTTACATCCAAATCCTAGCTTCTGTTCTTGGCAGTGAAGAAGCTGCTAAGAAAGCTCTTGCGTATAGTTTCAAGAGTTCCATGAGTGGCTTTGCAGCTAACCTGACTCCCGATCAAGTCAAGAAAATTTTGGCCCAACCAGGAGTTCTACATGTTGCACAAAGTGTTAACTATGATTTAACAGGAGGAAAAAGGAATAATGATGTATAG

Protein sequence

MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPKAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDLTGGKRNNDV

Homology

[Close]

BLAST of HG10022488 vs. NCBI nr
Match: XP_038896488.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 196.4 bits (498), Expect = 1.5e-46
Identity = 109/132 (82.58%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 1   MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKP 60
           MANTKM+FSSFTLYLVLLFTISLN LL SA ISDKS S IDV SSL++VYT ERPSNEKP
Sbjct: 1   MANTKMNFSSFTLYLVLLFTISLNSLLISAIISDKSASEIDVLSSLYIVYTTERPSNEKP 60

Query: 61  KAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNY 120
           +AFYIQILASVLGSEEAAKKAL YSFKSSM+GFAANLTPDQVKKI AQPGVL+  Q VNY
Sbjct: 61  EAFYIQILASVLGSEEAAKKALVYSFKSSMNGFAANLTPDQVKKISAQPGVLNFVQGVNY 120

Query: 121 DLTG-GKRNNDV 132
           +L   GK NNDV
Sbjct: 121 ELKATGKENNDV 132

[Close]

BLAST of HG10022488 vs. NCBI nr
Match: XP_038896487.1 (subtilisin-like protease SBT3.13 [Benincasa hispida])

HSP 1 Score: 192.2 bits (487), Expect = 2.8e-45
Identity = 105/127 (82.68%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 6   MSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPKAFYI 65
           M+FSSFTLYLVLLFTISLN LL SA ISDKS S IDVPSSLH+VYT ERPSNE+P+AFYI
Sbjct: 1   MNFSSFTLYLVLLFTISLNSLLISAIISDKSASKIDVPSSLHIVYTTERPSNEEPEAFYI 60

Query: 66  QILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDLTG- 125
           QILASVLGSEEAAKKAL YSFKSSM+GFAA+LTPDQVKKILAQPGVL+V Q VNY+L   
Sbjct: 61  QILASVLGSEEAAKKALVYSFKSSMNGFAADLTPDQVKKILAQPGVLNVLQGVNYELQAT 120

Query: 126 GKRNNDV 132
           G++NNDV
Sbjct: 121 GEKNNDV 127

[Close]

BLAST of HG10022488 vs. NCBI nr
Match: XP_008461963.1 (PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa] >TYK08050.1 subtilisin-like protease SBT3.4 [Cucumis melo var. makuwa])

HSP 1 Score: 184.1 bits (466), Expect = 7.7e-43
Identity = 100/132 (75.76%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKP 60
           M NTKM+FSS  LYLVLLFT+SLN LL    IS+KS S   VPSSLH+VYT +RPSNEKP
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61  KAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNY 120
           +AFYIQILASVLGS EAA+KAL YSFK+SM+GFAANLTP+QVKKI AQPGVLHVA+SV Y
Sbjct: 61  EAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTY 120

Query: 121 DL-TGGKRNNDV 132
           +L TGGK NN+V
Sbjct: 121 NLQTGGKENNNV 132

[Close]

BLAST of HG10022488 vs. NCBI nr
Match: ADN34190.1 (peptidase [Cucumis melo subsp. melo])

HSP 1 Score: 158.3 bits (399), Expect = 4.5e-35
Identity = 84/113 (74.34%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 20  TISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPKAFYIQILASVLGSEEAAK 79
           ++SLN LL    IS+KS S   VPSSLH+VYT +RPSNEKP+AFYIQILASVLGS EAA+
Sbjct: 20  SVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEAAR 79

Query: 80  KALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL-TGGKRNNDV 132
           KAL YSFK+SM+GFAANLTP+QVKKI AQPGVLHVA+SV Y+L TGGK NN+V
Sbjct: 80  KALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENNNV 132

[Close]

BLAST of HG10022488 vs. NCBI nr
Match: XP_038896852.1 (subtilisin-like protease SBT3.6 isoform X2 [Benincasa hispida])

HSP 1 Score: 131.0 bits (328), Expect = 7.7e-27
Identity = 68/89 (76.40%), Postives = 80/89 (89.89%), Query Frame = 0


Query: 44  SSLHLVYTIERPSNEKPKAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVK 103
           +++H+VYT ERP NE+P+A++I+ LASVLGSEEAAKKAL YSF SS +GFAANLTPDQV+
Sbjct: 6   TAVHIVYT-ERPHNEEPEAYHIRTLASVLGSEEAAKKALVYSFTSSTTGFAANLTPDQVE 65

Query: 104 KILAQPGVLHVAQSVNYDL-TGGKRNNDV 132
           KILAQPGVLHV QSVNYDL T GK+NNDV
Sbjct: 66  KILAQPGVLHVVQSVNYDLQTRGKKNNDV 93

[Close]

BLAST of HG10022488 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.7e-08
Identity = 41/109 (37.61%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 17  LLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPK---AFYIQILASVLG 76
           +L  +SL ++L  AR S K        S +H+VY  E+  ++ PK     + Q+L+S+LG
Sbjct: 8   VLVVLSLIIVLNVARASAK--------SKVHIVYLGEK-QHDDPKFVTESHHQMLSSLLG 67

Query: 77  SEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
           S++ A +++ YS++   SGFAA LT  Q KKI   P V+HV     Y+L
Sbjct: 68  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of HG10022488 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-07
Identity = 38/110 (34.55%), Postives = 62/110 (56.36%), Query Frame = 0


Query: 15  LVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVL 74
           ++L+  +SL ++L   R SD+        S +H+VY  E+  +  E     + Q+L+S+L
Sbjct: 6   VLLVLVLSLVIVLNVVRASDE--------SKVHIVYLGEKQHDDPEFVSESHHQMLSSLL 65

Query: 75  GSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
           GS+  A +++ YS++   SGFAA LT  Q KK+   P V+HV     Y+L
Sbjct: 66  GSKVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYEL 107

[Close]

BLAST of HG10022488 vs. ExPASy Swiss-Prot
Match: Q8GUK4 (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 2.4e-07
Identity = 41/102 (40.20%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 15  LVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVL 74
           LVLL  I+L L L +      +   +D  S +++VY  ER  +  E   A + Q+L S+L
Sbjct: 10  LVLLLAIALVLFLNTELDFLTAAGALDSDSKVYIVYLGEREHDDPELVTASHHQMLESLL 69

Query: 75  GSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHV 115
            S+E A+ +L YS++   SGFAA LT  Q KKI   P V+HV
Sbjct: 70  QSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHV 111

[Close]

BLAST of HG10022488 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.6e-06
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 16  VLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVLG 75
           +LL  +SL  +L + R   ++ S +      H+VY  E+  +  E     + Q+LAS+LG
Sbjct: 8   ILLVLLSLITVLNATRARSETESKV------HIVYLGEKKHHDPEFVTESHHQMLASLLG 67

Query: 76  SEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHV 115
           S++ A  ++ YS++   SGFAA LT  Q KKI   P V+HV
Sbjct: 68  SKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHV 102

[Close]

BLAST of HG10022488 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.7e-06
Identity = 27/81 (33.33%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 44  SSLHLVYTIERPSN--EKPKAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQ 103
           + +H+VY  E+  N  E   + ++++L S+LGS++ A +++ +S+++  SGFAA+LT  Q
Sbjct: 35  TKVHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQ 94

Query: 104 VKKILAQPGVLHVAQSVNYDL 123
            ++I   P V+ V  +  Y+L
Sbjct: 95  AEQISEHPDVVQVTPNTFYEL 115

[Close]

BLAST of HG10022488 vs. ExPASy TrEMBL
Match: A0A5D3C9P1 (Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001980 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.7e-43
Identity = 100/132 (75.76%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKP 60
           M NTKM+FSS  LYLVLLFT+SLN LL    IS+KS S   VPSSLH+VYT +RPSNEKP
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61  KAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNY 120
           +AFYIQILASVLGS EAA+KAL YSFK+SM+GFAANLTP+QVKKI AQPGVLHVA+SV Y
Sbjct: 61  EAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTY 120

Query: 121 DL-TGGKRNNDV 132
           +L TGGK NN+V
Sbjct: 121 NLQTGGKENNNV 132

[Close]

BLAST of HG10022488 vs. ExPASy TrEMBL
Match: A0A1S3CFT4 (subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.7e-43
Identity = 100/132 (75.76%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKP 60
           M NTKM+FSS  LYLVLLFT+SLN LL    IS+KS S   VPSSLH+VYT +RPSNEKP
Sbjct: 1   MTNTKMNFSSSPLYLVLLFTVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKP 60

Query: 61  KAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNY 120
           +AFYIQILASVLGS EAA+KAL YSFK+SM+GFAANLTP+QVKKI AQPGVLHVA+SV Y
Sbjct: 61  EAFYIQILASVLGSNEAARKALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTY 120

Query: 121 DL-TGGKRNNDV 132
           +L TGGK NN+V
Sbjct: 121 NLQTGGKENNNV 132

[Close]

BLAST of HG10022488 vs. ExPASy TrEMBL
Match: A0A0A0K630 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G274680 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.1e-42
Identity = 99/132 (75.00%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MANTKMSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKP 60
           M NTKM+FSSFTL LVLLFTISLN  L S  ISDK  S   VPSS+++VYT +RPSNEKP
Sbjct: 1   MTNTKMNFSSFTLSLVLLFTISLNSHLISGMISDKPVSKFVVPSSIYMVYTAKRPSNEKP 60

Query: 61  KAFYIQILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNY 120
           + FYIQILASV+ S EAAKKAL YSFKSS++GFAANLTP+QVKKILAQPGVLHVA+SVNY
Sbjct: 61  ETFYIQILASVIDSNEAAKKALVYSFKSSINGFAANLTPNQVKKILAQPGVLHVARSVNY 120

Query: 121 DL-TGGKRNNDV 132
           +L T GK+NN+V
Sbjct: 121 NLQTEGKKNNNV 132

[Close]

BLAST of HG10022488 vs. ExPASy TrEMBL
Match: E5GCJ1 (Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.2e-35
Identity = 84/113 (74.34%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 20  TISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPKAFYIQILASVLGSEEAAK 79
           ++SLN LL    IS+KS S   VPSSLH+VYT +RPSNEKP+AFYIQILASVLGS EAA+
Sbjct: 20  SVSLNSLLAWGMISEKSMSKFAVPSSLHIVYTAKRPSNEKPEAFYIQILASVLGSNEAAR 79

Query: 80  KALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL-TGGKRNNDV 132
           KAL YSFK+SM+GFAANLTP+QVKKI AQPGVLHVA+SV Y+L TGGK NN+V
Sbjct: 80  KALVYSFKNSMNGFAANLTPNQVKKISAQPGVLHVARSVTYNLQTGGKENNNV 132

[Close]

BLAST of HG10022488 vs. ExPASy TrEMBL
Match: A0A6J1IUK8 (subtilisin-like protease SBT3.6 OS=Cucurbita maxima OX=3661 GN=LOC111480709 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.1e-23
Identity = 67/117 (57.26%), Postives = 86/117 (73.50%), Query Frame = 0


Query: 6   MSFSSFTLYLVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPKAFYI 65
           ++FSSF  YLV  FTISL  LLTSA   DKS      PS +H++YT ERP NE P+A++I
Sbjct: 3   INFSSFPFYLVFFFTISLTFLLTSATQFDKSAPE-SAPSRVHIIYT-ERPLNENPEAYHI 62

Query: 66  QILASVLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
           +IL SVLGSE+AA++AL Y +K S+ GFAA LTPDQV+KI+ Q GVL+  +S +Y L
Sbjct: 63  RILTSVLGSEKAAREALVYCYKDSVYGFAAKLTPDQVEKIMKQAGVLNAVESHSYHL 117

[Close]

BLAST of HG10022488 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 85.9 bits (211), Expect = 2.7e-17
Identity = 48/112 (42.86%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 13  LYLVLLFTISLNLLLTSARISDKSTSNIDVPS--SLHLVYTIERPSNEKPKAFYIQILAS 72
           + +V  F   L+    S  ++D+ T      S   +H++YT E+P++E+PK ++++ L+S
Sbjct: 14  IIIVFFFFFCLSSFFASIVMADEYTGEATGSSEAKVHIIYT-EKPTDEEPKTYHLRTLSS 73

Query: 73  VLGSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
            LGSEEAAK AL YS+K + SGF+A LTP+QV +I  QPGV+ V  S  Y L
Sbjct: 74  ALGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQL 124

[Close]

BLAST of HG10022488 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.2e-09
Identity = 41/109 (37.61%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 17  LLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSNEKPK---AFYIQILASVLG 76
           +L  +SL ++L  AR S K        S +H+VY  E+  ++ PK     + Q+L+S+LG
Sbjct: 8   VLVVLSLIIVLNVARASAK--------SKVHIVYLGEK-QHDDPKFVTESHHQMLSSLLG 67

Query: 77  SEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
           S++ A +++ YS++   SGFAA LT  Q KKI   P V+HV     Y+L
Sbjct: 68  SKDDAHESMVYSYRHGFSGFAAKLTKSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of HG10022488 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.0e-08
Identity = 38/110 (34.55%), Postives = 62/110 (56.36%), Query Frame = 0


Query: 15  LVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVL 74
           ++L+  +SL ++L   R SD+        S +H+VY  E+  +  E     + Q+L+S+L
Sbjct: 6   VLLVLVLSLVIVLNVVRASDE--------SKVHIVYLGEKQHDDPEFVSESHHQMLSSLL 65

Query: 75  GSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHVAQSVNYDL 123
           GS+  A +++ YS++   SGFAA LT  Q KK+   P V+HV     Y+L
Sbjct: 66  GSKVDAHESMVYSYRHGFSGFAAKLTESQAKKLADSPEVVHVMADSFYEL 107

[Close]

BLAST of HG10022488 vs. TAIR 10
Match: AT4G21650.1 (Subtilase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.7e-08
Identity = 41/102 (40.20%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 15  LVLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVL 74
           LVLL  I+L L L +      +   +D  S +++VY  ER  +  E   A + Q+L S+L
Sbjct: 10  LVLLLAIALVLFLNTELDFLTAAGALDSDSKVYIVYLGEREHDDPELVTASHHQMLESLL 69

Query: 75  GSEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHV 115
            S+E A+ +L YS++   SGFAA LT  Q KKI   P V+HV
Sbjct: 70  QSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHV 111

[Close]

BLAST of HG10022488 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.1e-07
Identity = 37/101 (36.63%), Postives = 57/101 (56.44%), Query Frame = 0


Query: 16  VLLFTISLNLLLTSARISDKSTSNIDVPSSLHLVYTIERPSN--EKPKAFYIQILASVLG 75
           +LL  +SL  +L + R   ++ S +      H+VY  E+  +  E     + Q+LAS+LG
Sbjct: 8   ILLVLLSLITVLNATRARSETESKV------HIVYLGEKKHHDPEFVTESHHQMLASLLG 67

Query: 76  SEEAAKKALAYSFKSSMSGFAANLTPDQVKKILAQPGVLHV 115
           S++ A  ++ YS++   SGFAA LT  Q KKI   P V+HV
Sbjct: 68  SKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHV 102

The following BLAST results are available for this feature:

BLAST of HG10022488 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896488.1	1.5e-46	82.58	subtilisin-like protease SBT3.13 [Benincasa hispida]	[more]
XP_038896487.1	2.8e-45	82.68	subtilisin-like protease SBT3.13 [Benincasa hispida]	[more]
XP_008461963.1	7.7e-43	75.76	PREDICTED: subtilisin-like protease SBT3.4 [Cucumis melo] >KAA0048207.1 subtilis...	[more]
ADN34190.1	4.5e-35	74.34	peptidase [Cucumis melo subsp. melo]	[more]
XP_038896852.1	7.7e-27	76.40	subtilisin-like protease SBT3.6 isoform X2 [Benincasa hispida]	[more]

BLAST of HG10022488 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4HPF1	1.7e-08	37.61	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q9MAP7	1.4e-07	34.55	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]
Q8GUK4	2.4e-07	40.20	Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2...	[more]
Q9MAP5	1.6e-06	36.63	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q1PDX5	2.7e-06	33.33	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]

BLAST of HG10022488 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C9P1	3.7e-43	75.76	Subtilisin-like protease SBT3.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3CFT4	3.7e-43	75.76	subtilisin-like protease SBT3.4 OS=Cucumis melo OX=3656 GN=LOC103500439 PE=4 SV=...	[more]
A0A0A0K630	4.1e-42	75.00	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G27468...	[more]
E5GCJ1	2.2e-35	74.34	Peptidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A6J1IUK8	1.1e-23	57.26	subtilisin-like protease SBT3.6 OS=Cucurbita maxima OX=3661 GN=LOC111480709 PE=4...	[more]

BLAST of HG10022488 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	2.7e-17	42.86	Proteinase inhibitor, propeptide	[more]
AT1G32950.1	1.2e-09	37.61	Subtilase family protein	[more]
AT1G32940.1	1.0e-08	34.55	Subtilase family protein	[more]
AT4G21650.1	1.7e-08	40.20	Subtilase family protein	[more]
AT1G32960.1	1.1e-07	36.63	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 29..122 e-value: 4.3E-16 score: 61.1
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 65..121 e-value: 8.8E-10 score: 39.1
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 37..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022488.1	HG10022488.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity