HG10022449 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022449
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGDP-L-galactose phosphorylase 1-like
LocationChr05: 24403405 .. 24403827 (-)
RNA-Seq ExpressionHG10022449
SyntenyHG10022449
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAACATTCACTCTTAATTTCCTGTGTTCTTGTTATAGTTCTGACCTTGCTAATCATAATGACCAGTGTTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGCGGAGCTCCTCGACACCCAAGTGAACCCAGCCGTTTGGGAGATAAGTGGTCACATGGTGTTGAAGAGGAAAAAGGATTACGAAGAGGCATCGGAGGAAAATGCGTGGAGGCTCCTCGCCGAGGTGTCCCTATCGGAAGAGAGATTTCAAGAAGTGTGTGCTCTCATCTTCGAAGTTATCGATATGGTTGATGCTGAAGATGCTGAAGGTAGCCCGAGCATGCCTGAGGAGCATGGTGATGCTTGTCTCAAGGATGTGGAAGCCAGAAACGCTTCCCGCCCACTCTCGGTGACTGGGACGAAGGAATGCTTAGTTTAG

mRNA sequence

ATGATAACATTCACTCTTAATTTCCTGTGTTCTTGTTATAGTTCTGACCTTGCTAATCATAATGACCAGTGTTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGCGGAGCTCCTCGACACCCAAGTGAACCCAGCCGTTTGGGAGATAAGTGGTCACATGGTGTTGAAGAGGAAAAAGGATTACGAAGAGGCATCGGAGGAAAATGCGTGGAGGCTCCTCGCCGAGGTGTCCCTATCGGAAGAGAGATTTCAAGAAGTGTGTGCTCTCATCTTCGAAGTTATCGATATGGTTGATGCTGAAGATGCTGAAGGTAGCCCGAGCATGCCTGAGGAGCATGGTGATGCTTGTCTCAAGGATGTGGAAGCCAGAAACGCTTCCCGCCCACTCTCGGTGACTGGGACGAAGGAATGCTTAGTTTAG

Coding sequence (CDS)

ATGATAACATTCACTCTTAATTTCCTGTGTTCTTGTTATAGTTCTGACCTTGCTAATCATAATGACCAGTGTTATGCTGAGAAACAAGCACTTGGGGAAGTGAGCGCGGAGCTCCTCGACACCCAAGTGAACCCAGCCGTTTGGGAGATAAGTGGTCACATGGTGTTGAAGAGGAAAAAGGATTACGAAGAGGCATCGGAGGAAAATGCGTGGAGGCTCCTCGCCGAGGTGTCCCTATCGGAAGAGAGATTTCAAGAAGTGTGTGCTCTCATCTTCGAAGTTATCGATATGGTTGATGCTGAAGATGCTGAAGGTAGCCCGAGCATGCCTGAGGAGCATGGTGATGCTTGTCTCAAGGATGTGGAAGCCAGAAACGCTTCCCGCCCACTCTCGGTGACTGGGACGAAGGAATGCTTAGTTTAG

Protein sequence

MITFTLNFLCSCYSSDLANHNDQCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEERFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARNASRPLSVTGTKECLV
Homology
BLAST of HG10022449 vs. NCBI nr
Match: XP_004139797.1 (GDP-L-galactose phosphorylase 1 [Cucumis sativus] >KGN44184.1 hypothetical protein Csa_015961 [Cucumis sativus])

HSP 1 Score: 208.4 bits (529), Expect = 4.1e-50
Identity = 106/119 (89.08%), Postives = 110/119 (92.44%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLA VSLSEE
Sbjct: 327 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAVVSLSEE 386

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEVID VDAE+ EGS SMPEEH D CLK+VEAR N +RPL +TGTKECLV
Sbjct: 387 RFQEVCALIFEVIDTVDAENTEGSSSMPEEHDDGCLKEVEARNNTTRPLPMTGTKECLV 445

BLAST of HG10022449 vs. NCBI nr
Match: XP_038897875.1 (GDP-L-galactose phosphorylase 2 [Benincasa hispida])

HSP 1 Score: 208.4 bits (529), Expect = 4.1e-50
Identity = 107/113 (94.69%), Postives = 109/113 (96.46%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE
Sbjct: 327 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 386

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARN-ASRPLSVTG 135
           RFQEVCALIFEVID VDAE+AEGSP MPEEH DACLK+VEARN ASRPLSVTG
Sbjct: 387 RFQEVCALIFEVIDTVDAENAEGSPRMPEEHDDACLKEVEARNKASRPLSVTG 439

BLAST of HG10022449 vs. NCBI nr
Match: XP_023534897.1 (GDP-L-galactose phosphorylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.0 bits (515), Expect = 1.7e-48
Identity = 104/119 (87.39%), Postives = 111/119 (93.28%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDY+EASEENAWRLLAEVSLSEE
Sbjct: 325 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEE 384

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEV+D V   D EGSPS+ +EHGDACL++VEAR NASRPL VTGT+ECLV
Sbjct: 385 RFQEVCALIFEVLDTV---DVEGSPSVAKEHGDACLEEVEARNNASRPLQVTGTRECLV 440

BLAST of HG10022449 vs. NCBI nr
Match: XP_022936265.1 (GDP-L-galactose phosphorylase 1-like [Cucurbita moschata])

HSP 1 Score: 202.2 bits (513), Expect = 2.9e-48
Identity = 105/119 (88.24%), Postives = 110/119 (92.44%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDY+EASEENAWRLLAEVSLSEE
Sbjct: 324 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEE 383

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEV+D V   D EGSPS+ EEHGDACL++VEAR NASRPL VTGT ECLV
Sbjct: 384 RFQEVCALIFEVLDTV---DVEGSPSVAEEHGDACLEEVEARNNASRPLRVTGTWECLV 439

BLAST of HG10022449 vs. NCBI nr
Match: KAG6592057.1 (GDP-L-galactose phosphorylase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 202.2 bits (513), Expect = 2.9e-48
Identity = 105/119 (88.24%), Postives = 110/119 (92.44%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDY+EASEENAWRLLAEVSLSEE
Sbjct: 325 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEE 384

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEV+D V   D EGSPS+ EEHGDACL++VEAR NASRPL VTGT ECLV
Sbjct: 385 RFQEVCALIFEVLDTV---DVEGSPSVAEEHGDACLEEVEARNNASRPLRVTGTWECLV 440

BLAST of HG10022449 vs. ExPASy Swiss-Prot
Match: Q8RWE8 (GDP-L-galactose phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=VTC2 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 8.3e-30
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS E+L+TQVNPAVWEISGHMVLKRK+DYE ASE+NAWRLLAE SLSEE
Sbjct: 323 QCYAEKQALGEVSPEVLETQVNPAVWEISGHMVLKRKEDYEGASEDNAWRLLAEASLSEE 382

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARNASRPLSVTGTKECLV 141
           RF+EV AL FE I   + E+      + +++    +     R    P++     ECLV
Sbjct: 383 RFKEVTALAFEAIGCSNQEEDLEGTIVHQQNSSGNVNQKSNRTHGGPITNGTAAECLV 440

BLAST of HG10022449 vs. ExPASy Swiss-Prot
Match: Q9FLP9 (GDP-L-galactose phosphorylase 2 OS=Arabidopsis thaliana OX=3702 GN=VTC5 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 5.9e-28
Identity = 63/82 (76.83%), Postives = 70/82 (85.37%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS+ LLDTQVNPAVWE+SGHMVLKRK+DYE ASEE AWRLLAEVSLSEE
Sbjct: 320 QCYAEKQALGEVSSTLLDTQVNPAVWEMSGHMVLKRKEDYEGASEEKAWRLLAEVSLSEE 379

Query: 83  RFQEVCALIFEVIDMVDAEDAE 105
           RF+EV  +IF+ I     E+ E
Sbjct: 380 RFREVNTMIFDAIGFSSHEEEE 401

BLAST of HG10022449 vs. ExPASy TrEMBL
Match: A0A0A0K5F4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219200 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.0e-50
Identity = 106/119 (89.08%), Postives = 110/119 (92.44%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLA VSLSEE
Sbjct: 327 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAVVSLSEE 386

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEVID VDAE+ EGS SMPEEH D CLK+VEAR N +RPL +TGTKECLV
Sbjct: 387 RFQEVCALIFEVIDTVDAENTEGSSSMPEEHDDGCLKEVEARNNTTRPLPMTGTKECLV 445

BLAST of HG10022449 vs. ExPASy TrEMBL
Match: A0A6J1FD57 (GDP-L-galactose phosphorylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442936 PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.4e-48
Identity = 105/119 (88.24%), Postives = 110/119 (92.44%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDY+EASEENAWRLLAEVSLSEE
Sbjct: 324 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYDEASEENAWRLLAEVSLSEE 383

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEAR-NASRPLSVTGTKECLV 141
           RFQEVCALIFEV+D V   D EGSPS+ EEHGDACL++VEAR NASRPL VTGT ECLV
Sbjct: 384 RFQEVCALIFEVLDTV---DVEGSPSVAEEHGDACLEEVEARNNASRPLRVTGTWECLV 439

BLAST of HG10022449 vs. ExPASy TrEMBL
Match: A0A6J1J1Y3 (GDP-L-galactose phosphorylase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480581 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-47
Identity = 100/118 (84.75%), Postives = 109/118 (92.37%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS+ELL+TQVNPAVWEISGHMVLKRK+DYEEASEENAWRLLAEVSLSEE
Sbjct: 328 QCYAEKQALGEVSSELLETQVNPAVWEISGHMVLKRKEDYEEASEENAWRLLAEVSLSEE 387

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARNASRPLSVTGTKECLV 141
           RFQEVCALIFEVI+  DAE+AEGSPS P+E  DAC+K VEA+NAS  L VTGT+ECLV
Sbjct: 388 RFQEVCALIFEVINTGDAENAEGSPSCPKEESDACVKKVEAKNASPRLPVTGTQECLV 445

BLAST of HG10022449 vs. ExPASy TrEMBL
Match: A0A6J1FJ15 (GDP-L-galactose phosphorylase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111446172 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.2e-47
Identity = 100/118 (84.75%), Postives = 109/118 (92.37%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS+ELL+TQVNPAVWEISGHMVLKRK+DYEEASEENAWRLLAEVSLSEE
Sbjct: 328 QCYAEKQALGEVSSELLETQVNPAVWEISGHMVLKRKEDYEEASEENAWRLLAEVSLSEE 387

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARNASRPLSVTGTKECLV 141
           RFQEVCALIFEVI+  DAE+AEGSPS P+E  DAC+K VEA+NAS  L VTGT+ECLV
Sbjct: 388 RFQEVCALIFEVINTGDAENAEGSPSCPKEQSDACVKKVEAKNASPRLPVTGTQECLV 445

BLAST of HG10022449 vs. ExPASy TrEMBL
Match: A0A1S3BIN8 (GDP-L-galactose phosphorylase 1 OS=Cucumis melo OX=3656 GN=LOC103490128 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.0e-47
Identity = 106/126 (84.13%), Postives = 108/126 (85.71%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE
Sbjct: 327 QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 386

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMP-------EEHGDACLKDVEAR-NASRPLSVTG 141
           RFQEVCALIFEVID VDAE+ E S SMP       EEHGD CLK VEAR N +  L VTG
Sbjct: 387 RFQEVCALIFEVIDTVDAENTEDSSSMPEEHGDGCEEHGDGCLKVVEARNNTTHSLPVTG 446

BLAST of HG10022449 vs. TAIR 10
Match: AT4G26850.1 (mannose-1-phosphate guanylyltransferase (GDP)s;GDP-galactose:mannose-1-phosphate guanylyltransferases;GDP-galactose:glucose-1-phosphate guanylyltransferases;GDP-galactose:myoinositol-1-phosphate guanylyltransferases;glucose-1-phosphate guanylyltransferase )

HSP 1 Score: 131.3 bits (329), Expect = 5.9e-31
Identity = 69/118 (58.47%), Postives = 83/118 (70.34%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS E+L+TQVNPAVWEISGHMVLKRK+DYE ASE+NAWRLLAE SLSEE
Sbjct: 323 QCYAEKQALGEVSPEVLETQVNPAVWEISGHMVLKRKEDYEGASEDNAWRLLAEASLSEE 382

Query: 83  RFQEVCALIFEVIDMVDAEDAEGSPSMPEEHGDACLKDVEARNASRPLSVTGTKECLV 141
           RF+EV AL FE I   + E+      + +++    +     R    P++     ECLV
Sbjct: 383 RFKEVTALAFEAIGCSNQEEDLEGTIVHQQNSSGNVNQKSNRTHGGPITNGTAAECLV 440

BLAST of HG10022449 vs. TAIR 10
Match: AT5G55120.1 (galactose-1-phosphate guanylyltransferase (GDP)s;GDP-D-glucose phosphorylases;quercetin 4'-O-glucosyltransferases )

HSP 1 Score: 125.2 bits (313), Expect = 4.2e-29
Identity = 63/82 (76.83%), Postives = 70/82 (85.37%), Query Frame = 0

Query: 23  QCYAEKQALGEVSAELLDTQVNPAVWEISGHMVLKRKKDYEEASEENAWRLLAEVSLSEE 82
           QCYAEKQALGEVS+ LLDTQVNPAVWE+SGHMVLKRK+DYE ASEE AWRLLAEVSLSEE
Sbjct: 320 QCYAEKQALGEVSSTLLDTQVNPAVWEMSGHMVLKRKEDYEGASEEKAWRLLAEVSLSEE 379

Query: 83  RFQEVCALIFEVIDMVDAEDAE 105
           RF+EV  +IF+ I     E+ E
Sbjct: 380 RFREVNTMIFDAIGFSSHEEEE 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004139797.14.1e-5089.08GDP-L-galactose phosphorylase 1 [Cucumis sativus] >KGN44184.1 hypothetical prote... [more]
XP_038897875.14.1e-5094.69GDP-L-galactose phosphorylase 2 [Benincasa hispida][more]
XP_023534897.11.7e-4887.39GDP-L-galactose phosphorylase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022936265.12.9e-4888.24GDP-L-galactose phosphorylase 1-like [Cucurbita moschata][more]
KAG6592057.12.9e-4888.24GDP-L-galactose phosphorylase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q8RWE88.3e-3058.47GDP-L-galactose phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=VTC2 PE=1 SV=... [more]
Q9FLP95.9e-2876.83GDP-L-galactose phosphorylase 2 OS=Arabidopsis thaliana OX=3702 GN=VTC5 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0K5F42.0e-5089.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G219200 PE=3 SV=1[more]
A0A6J1FD571.4e-4888.24GDP-L-galactose phosphorylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A6J1J1Y31.2e-4784.75GDP-L-galactose phosphorylase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111480581... [more]
A0A6J1FJ151.2e-4784.75GDP-L-galactose phosphorylase 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114461... [more]
A0A1S3BIN82.0e-4784.13GDP-L-galactose phosphorylase 1 OS=Cucumis melo OX=3656 GN=LOC103490128 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT4G26850.15.9e-3158.47mannose-1-phosphate guanylyltransferase (GDP)s;GDP-galactose:mannose-1-phosphate... [more]
AT5G55120.14.2e-2976.83galactose-1-phosphate guanylyltransferase (GDP)s;GDP-D-glucose phosphorylases;qu... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR20884:SF17GDP-L-GALACTOSE PHOSPHORYLASE 2coord: 23..105
IPR026506GDP-L-galactose/GDP-D-glucose phosphorylasePANTHERPTHR20884GDP-D-GLUCOSE PHOSPHORYLASE 1coord: 23..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022449.1HG10022449.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0080048 GDP-D-glucose phosphorylase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0016779 nucleotidyltransferase activity