HG10022434 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022434
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationChr05: 24237588 .. 24238529 (+)
RNA-Seq ExpressionHG10022434
SyntenyHG10022434
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGCGAAATCATAAGATTCATCAAGGTGTTGCCCATATTCATTGTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTCTCCAAAGGCAAACTTAGGCTCCTCTCTCTCCTCCCCATCTTCTCTCTCTTCGTGGTCGTCCCTCTCTACTTCTCCTCCATCCTCTTCACGAGCAGCGTCGCCTTATTCGTCACCTGGCTTACGACCTTCAAGCTCCTTCTTTTCTCATTCGATTCAGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAGTCAAGCATAACAAAACAACCCCACATGACCAAGAACCACCTCCAAAGTTGCCTCTAAACTTGCCAACCAAAGTGGTGATATGTGCCATATTGGTCTTGGGAAACAACTACATTGACCTAGTTTGTCCCAATAATGCAAAGTTATGGATCAATTGTGCTACATTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGCTTTTGTTCGATTGACTTTCGGCATCGAGGTCAGACAACCATTCAACGAGCCGTATCTGGCAACGTCGCTTCAAAATTTGTGGGGTCGTAGGTGGAATCTATTAGTATCTGAAATATTACACAACACAATCTACAAACCAGTTCGTTACAAAGTAGGGGTCCCACGGTGGGCGGCGGTGATAATGGTATTTGTTGTGTCGGGACTTATGCACGAACTTTTGTATTACTATATTATCAGAGCGATTCCAACGTGGGAAGCCACGTGGTTCTTTGTTATTCATGGAGTGTGTTTGGCCATGGAGATTGAATTGAAGAGAGCATATGGAAAAAGGTGGCGGTTGCATTGGGCAGTTTCTGGACCATTAACGACGACGTTTATGGCAACCACCGCTTATTGGTTGCTTTTTCCATTAATGTTGAGGTCTTGA

mRNA sequence

ATGGGTAGCGAAATCATAAGATTCATCAAGGTGTTGCCCATATTCATTGTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTCTCCAAAGGCAAACTTAGGCTCCTCTCTCTCCTCCCCATCTTCTCTCTCTTCGTGGTCGTCCCTCTCTACTTCTCCTCCATCCTCTTCACGAGCAGCGTCGCCTTATTCGTCACCTGGCTTACGACCTTCAAGCTCCTTCTTTTCTCATTCGATTCAGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAGTCAAGCATAACAAAACAACCCCACATGACCAAGAACCACCTCCAAAGTTGCCTCTAAACTTGCCAACCAAAGTGGTGATATGTGCCATATTGGTCTTGGGAAACAACTACATTGACCTAGTTTGTCCCAATAATGCAAAGTTATGGATCAATTGTGCTACATTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGCTTTTGTTCGATTGACTTTCGGCATCGAGGTCAGACAACCATTCAACGAGCCGTATCTGGCAACGTCGCTTCAAAATTTGTGGGGTCGTAGGTGGAATCTATTAGTATCTGAAATATTACACAACACAATCTACAAACCAGTTCGTTACAAAGTAGGGGTCCCACGGTGGGCGGCGGTGATAATGGTATTTGTTGTGTCGGGACTTATGCACGAACTTTTGTATTACTATATTATCAGAGCGATTCCAACGTGGGAAGCCACGTGGTTCTTTGTTATTCATGGAGTGTGTTTGGCCATGGAGATTGAATTGAAGAGAGCATATGGAAAAAGGTGGCGGTTGCATTGGGCAGTTTCTGGACCATTAACGACGACGTTTATGGCAACCACCGCTTATTGGTTGCTTTTTCCATTAATGTTGAGGTCTTGA

Coding sequence (CDS)

ATGGGTAGCGAAATCATAAGATTCATCAAGGTGTTGCCCATATTCATTGTGTCCTCTTTGTATTGCTATTTCATAGCTTCAAAGTTCTCCAAAGGCAAACTTAGGCTCCTCTCTCTCCTCCCCATCTTCTCTCTCTTCGTGGTCGTCCCTCTCTACTTCTCCTCCATCCTCTTCACGAGCAGCGTCGCCTTATTCGTCACCTGGCTTACGACCTTCAAGCTCCTTCTTTTCTCATTCGATTCAGGTCCTCTAGCTTCCAACCCACCATTGTCATTCCCTCTTTTTGCTTCCATTGCTTTCTTTCCAACTAGAGTCAAGCATAACAAAACAACCCCACATGACCAAGAACCACCTCCAAAGTTGCCTCTAAACTTGCCAACCAAAGTGGTGATATGTGCCATATTGGTCTTGGGAAACAACTACATTGACCTAGTTTGTCCCAATAATGCAAAGTTATGGATCAATTGTGCTACATTGTATTTCTACTTAGACGTAGTTATGAGTTTGTCCAATGCTTTTGTTCGATTGACTTTCGGCATCGAGGTCAGACAACCATTCAACGAGCCGTATCTGGCAACGTCGCTTCAAAATTTGTGGGGTCGTAGGTGGAATCTATTAGTATCTGAAATATTACACAACACAATCTACAAACCAGTTCGTTACAAAGTAGGGGTCCCACGGTGGGCGGCGGTGATAATGGTATTTGTTGTGTCGGGACTTATGCACGAACTTTTGTATTACTATATTATCAGAGCGATTCCAACGTGGGAAGCCACGTGGTTCTTTGTTATTCATGGAGTGTGTTTGGCCATGGAGATTGAATTGAAGAGAGCATATGGAAAAAGGTGGCGGTTGCATTGGGCAGTTTCTGGACCATTAACGACGACGTTTATGGCAACCACCGCTTATTGGTTGCTTTTTCCATTAATGTTGAGGTCTTGA

Protein sequence

MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTSSVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPKLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMATTAYWLLFPLMLRS
Homology
BLAST of HG10022434 vs. NCBI nr
Match: XP_038897379.1 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 1 [Benincasa hispida])

HSP 1 Score: 540.0 bits (1390), Expect = 1.3e-149
Identity = 265/313 (84.66%), Postives = 285/313 (91.05%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           MG EIIRFIK+LPIFI+SSLYCYFIASKFSKGKLRLLSLLP+FSLF V+PLYFSSILFTS
Sbjct: 1   MGGEIIRFIKMLPIFIMSSLYCYFIASKFSKGKLRLLSLLPVFSLFTVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           ++ALFVTWLTTFKLLLFSFD GPLAS+PPLSFPLFASIAFFPTRVKHNKTTP DQEPPPK
Sbjct: 61  NIALFVTWLTTFKLLLFSFDLGPLASDPPLSFPLFASIAFFPTRVKHNKTTPPDQEPPPK 120

Query: 121 LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGI 180
           LPLNLP+KVVI AIL+LGN+YI LVCPNN K WINC T+YFYLDV+MSLSN FVR TFG+
Sbjct: 121 LPLNLPSKVVILAILILGNSYIVLVCPNNVKFWINCVTIYFYLDVIMSLSNVFVRSTFGV 180

Query: 181 EVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSGL 240
           EVRQ FNEPYL+TSLQNLWGRRWNLLVSE LHNTIYKPVRYKVG+PRW       V   L
Sbjct: 181 EVRQAFNEPYLSTSLQNLWGRRWNLLVSETLHNTIYKPVRYKVGMPRW-------VAXEL 240

Query: 241 MHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMAT 300
           ++ELLYYYI+RA PTWEATWFFV+HGVCLA+EIELKRAYGKRW LHWAVSGPLTT FM T
Sbjct: 241 LYELLYYYIVRASPTWEATWFFVMHGVCLAVEIELKRAYGKRWPLHWAVSGPLTTVFMVT 300

Query: 301 TAYWLLFPLMLRS 314
           T YWLLFPLMLRS
Sbjct: 301 TTYWLLFPLMLRS 306

BLAST of HG10022434 vs. NCBI nr
Match: XP_004140072.1 (probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativus] >KGN44002.1 hypothetical protein Csa_015964 [Cucumis sativus])

HSP 1 Score: 524.2 bits (1349), Expect = 7.5e-145
Identity = 261/314 (83.12%), Postives = 285/314 (90.76%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  EI+RFIKVLP+FI+SSLYCYFIASKFSK KLRLLSLLP+FS+FVV+PLYFSSILFTS
Sbjct: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLLF+FDSGPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQ+  PK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            LPLNLPTKVVI AIL+LGN+YIDLVCPNN K WIN AT+YFYLDVVMSLSN FV+ TFG
Sbjct: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNLLVSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYY++RA PTWE TWFFVIHGVCLA+EIELKRAY K+W+L  AVS  LTT F  
Sbjct: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  WLLFPLML++
Sbjct: 301 TTTLWLLFPLMLKT 314

BLAST of HG10022434 vs. NCBI nr
Match: KAA0048128.1 (putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa] >TYK23044.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 258/314 (82.17%), Postives = 283/314 (90.13%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M +EI+RFI VLPIFI+SSLYCYF+ASKFSK KLRLLSLLP+FS+F+V+PLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLLF+FD+GPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQE  PK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            L L+LPTKVVI AIL+LGN+YIDLVCPN  K WINCAT+YFYLDVVMSLSN FVR TFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNL+VSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYYI+RAIPTWE  WFFVIHGVCLA+EIELK+AY KRW+L  A S  LTTTF A
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  W LFP MLR+
Sbjct: 301 TTTLWFLFPPMLRT 314

BLAST of HG10022434 vs. NCBI nr
Match: XP_008447704.1 (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 516.5 bits (1329), Expect = 1.6e-142
Identity = 257/314 (81.85%), Postives = 282/314 (89.81%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M +EI+RFI VLPIFI+SSLYCYF+ASKFSK KLRLLSLLP+FS+F+V+PLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLL +FD+GPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQE  PK
Sbjct: 61  SVGLFVTWLTTFKLLLVTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            L L+LPTKVVI AIL+LGN+YIDLVCPN  K WINCAT+YFYLDVVMSLSN FVR TFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNL+VSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYYI+RAIPTWE  WFFVIHGVCLA+EIELK+AY KRW+L  A S  LTTTF A
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  W LFP MLR+
Sbjct: 301 TTTLWFLFPPMLRT 314

BLAST of HG10022434 vs. NCBI nr
Match: XP_038899290.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 439.5 bits (1129), Expect = 2.4e-119
Identity = 225/326 (69.02%), Postives = 253/326 (77.61%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  EIIRFIKVL I + S LYCY IASK  KGKLRLLSLLPIF LFVV+PL  S ++ TS
Sbjct: 1   MDGEIIRFIKVLLICMASLLYCYSIASKLPKGKLRLLSLLPIFPLFVVLPLDLSYVVLTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPH------- 120
            + +FVTWLTTFKLL FSFD GPLAS+PPLSFPLFASIAF PTR+K  KTTP        
Sbjct: 61  GITIFVTWLTTFKLLQFSFDLGPLASDPPLSFPLFASIAFLPTRIKDIKTTPSVQNHRKD 120

Query: 121 -------DQEPPPKLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVV 180
                  DQEPPPKLPLNLP KV + A+LV G +Y+DLV P   KL  NC  LYFYLD+V
Sbjct: 121 SNTPQELDQEPPPKLPLNLPAKVGLFALLVFGKSYVDLVSP-IVKLCFNCGILYFYLDMV 180

Query: 181 MSLSNAFVRLTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVP 240
           MSLSN FVR +FG+EVR+ F+EPYLATSLQN WGRRWN LVSE LHNTIYKPVRY VG P
Sbjct: 181 MSLSNIFVRSSFGVEVRRSFDEPYLATSLQNFWGRRWNRLVSETLHNTIYKPVRYDVGSP 240

Query: 241 RWAAVIMVFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLH 300
           RW AV +VFVVSGLMHELL+YY+IR +PTWE TWFF++HGVC+A EIELK A GK+W  H
Sbjct: 241 RWMAVFVVFVVSGLMHELLFYYVIRVVPTWEVTWFFIVHGVCVAAEIELKSALGKKWHFH 300

Query: 301 WAVSGPLTTTFMATTAYWLLFPLMLR 313
            AV+GPLT  FM  TA+WL FP +LR
Sbjct: 301 GAVTGPLTMAFMGVTAHWLFFPQLLR 325

BLAST of HG10022434 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.8e-53
Identity = 128/321 (39.88%), Postives = 184/321 (57.32%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+   IKV    I+S  YCY+I  +   G  R LS+ P+ +LF+V+PL+FSS+  + 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
             A F+TWL  FKL+LFSFD GPL    P +FP F     FP +V+ N   P  Q   PK
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIP-IPTNFPRFFCFTCFPIKVQQN---PKSQNHLPK 120

Query: 121 LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGI 180
           L   +  K+ I A+L+   +Y   + P    L +    LY  ++++++     V ++ G 
Sbjct: 121 LVFAI--KLAIFAVLLHLYSYRQNLSP-TILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 181 EVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR------YKVGVPRWAAVIMV 240
           ++    N+PYLATSLQ+ WGRRWNL+V  IL   +Y P+R         G   +  ++  
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 241 FVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKR--AYGKRWRLHWAVSGP 300
           F+VSGL+HELL++Y+IR +PT E T FFV+HGVC A+E+ +K+     +RWRL   VS  
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRV 300

Query: 301 LTTTFMATTAYWLLFPLMLRS 314
           LT  F+  T  WL  P + RS
Sbjct: 301 LTVGFVFVTGGWLFTPQLKRS 314

BLAST of HG10022434 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.1e-53
Identity = 135/324 (41.67%), Postives = 185/324 (57.10%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  +    I+V    ++S  YCY+I+SK SKG LRLLS+LP+  LF+V+PL+ S + F +
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
              LF++WL  FKLLLF+FD GPL   PP     F   A  P +++ +   P     P  
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQD---PSPNAIPNL 120

Query: 121 LPLNLPTKVVICAILVLG-----NNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVR 180
            P  +P  V+   ILVLG       Y D + P    L + C  +Y  +++V+    A V 
Sbjct: 121 HPKPMPKWVLAVKILVLGVLLHVYEYRDGL-PRFVVLALYCLHIYLEVELVLVFVGAVVS 180

Query: 181 LTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR---YKVGVP---RWA 240
              G  +   FNEPYLATSLQ+ W RRWNL+VS +L +T++ PV+    ++  P    +A
Sbjct: 181 TLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFA 240

Query: 241 AVIMVFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAV 300
            V+  F VSGLMHELLY+Y+IR  PTWE T FFV+HG   A EI +KR    R   H AV
Sbjct: 241 GVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR-PPHRAV 300

Query: 301 SGPLTTTFMATTAYWLLFPLMLRS 314
           SG +  TF++ T  WL    +LR+
Sbjct: 301 SGLVVLTFVSVTGVWLFLAQVLRN 318

BLAST of HG10022434 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 9.1e-53
Identity = 118/309 (38.19%), Postives = 174/309 (56.31%), Query Frame = 0

Query: 8   FIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTSSVALFVT 67
           FIK   + I+S  Y +FIA    KG  RL+   P+F +F +VP    S+      A F+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFSFDSGPLASN-PPLSFPLFASIAFFPTRVK--HNKTTPHDQEPPPKLPLN 127
           WL  FKLLLF+   GPL+SN  PLS P+F +++  P +++     T  H  E   + PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 LPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGIEVRQ 187
              K V   +++    Y     P    L +    +YF L+++++ + A VR    +E+  
Sbjct: 124 YTIKAVFVVLIIKAYEY-STKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 183

Query: 188 PFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKP--VRYKVGVPRWAAVIM---VFVVSG 247
            FN+PYLATSLQ+ WGRRWNL+V+ IL  T+Y+P    + V  P ++ ++     FVVSG
Sbjct: 184 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 307
           +MHEL+++Y+ R  P W+  WFF+I+G C  +EI +K+    RWR   A+S  LT TF+ 
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTAYWLLFP 309
            TA WL  P
Sbjct: 304 VTALWLFLP 311

BLAST of HG10022434 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.2e-52
Identity = 132/322 (40.99%), Postives = 183/322 (56.83%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+   IKV    ++S  YCY+I+SK SKG LRLLSLLPIF +F+++PL+FSS+ F  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPH-DQEPPP 120
               F TWL  FKL LF+FD  PL S  P +   F   A FP ++  N ++     +P  
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKPMS 120

Query: 121 KLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
           K  L     +    + V  NNY D      A L +    +Y   ++++    A + +  G
Sbjct: 121 KWVLAFKLLIFSFLLHVYRNNY-DSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLLG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRY----KVGVPR--WAAVIM 240
            E+   FN+PYLATSLQ  W RRWNL+V  +L   ++ PV+      +G+ R  +A ++ 
Sbjct: 181 CEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGMLA 240

Query: 241 VFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRW--RLHWAVSG 300
            F+VSGLMHEL+Y+Y+IR  PTWE T FF++HGV   +EI +KR    RW      AVSG
Sbjct: 241 TFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKR---MRWLPTPRRAVSG 300

Query: 301 PLTTTFMATTAYWLLFPLMLRS 314
              T F+  TA WL +P MLR+
Sbjct: 301 LAITVFLLVTAGWLFYPQMLRN 317

BLAST of HG10022434 vs. ExPASy Swiss-Prot
Match: Q9FJ77 (Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=AT6 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.5e-52
Identity = 123/318 (38.68%), Postives = 182/318 (57.23%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+  FI+V    I+S  YCY++  K     LRLLS+LP+  LF+++P++FS++  + 
Sbjct: 1   MEEELKLFIQVWVSAIISVTYCYYLTPKIKTSLLRLLSVLPVCVLFLIIPIFFSTVHSSF 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           ++A F++ L   KL+LF+ + GPL   PP + P F   A FP +++  K  P +    PK
Sbjct: 61  TIAFFLSGLAVPKLILFALEKGPLFPLPP-NLPHFVCFACFPIKLQ-KKPNPENTNHFPK 120

Query: 121 LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGI 180
               L  KV I   L+L   +       N  L +    +Y  L++ ++L    V +T G 
Sbjct: 121 WVFAL--KVFIFGALLLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLGC 180

Query: 181 EVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR-YKVGVPRW---AAVIMVFV 240
           ++   FNEPYLATSL + WG RWNL+VS+IL   +Y P+R ++     W    A+   F+
Sbjct: 181 DLEPQFNEPYLATSLHDFWGHRWNLMVSKILWLAVYNPIRQWRAKSSEWDRFFAIFATFL 240

Query: 241 VSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKR--AYGKRWRLHWAVSGPLT 300
           VSG+ HE+LY+Y+ R  PTWE TWFFV+HG C+A E+ LKR     +RW ++ AVS  LT
Sbjct: 241 VSGVAHEILYFYLTREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLLT 300

Query: 301 TTFMATTAYWLLFPLMLR 313
             F+  T  WL  P  +R
Sbjct: 301 VGFVFVTGVWLFSPQPIR 314

BLAST of HG10022434 vs. ExPASy TrEMBL
Match: A0A0A0K6W1 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091220 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.6e-145
Identity = 261/314 (83.12%), Postives = 285/314 (90.76%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  EI+RFIKVLP+FI+SSLYCYFIASKFSK KLRLLSLLP+FS+FVV+PLYFSSILFTS
Sbjct: 1   MVGEIVRFIKVLPVFILSSLYCYFIASKFSKAKLRLLSLLPVFSIFVVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLLF+FDSGPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQ+  PK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDSGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQKSLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            LPLNLPTKVVI AIL+LGN+YIDLVCPNN K WIN AT+YFYLDVVMSLSN FV+ TFG
Sbjct: 121 LLPLNLPTKVVILAILILGNSYIDLVCPNNVKSWINGATVYFYLDVVMSLSNVFVQSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNLLVSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLLVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYY++RA PTWE TWFFVIHGVCLA+EIELKRAY K+W+L  AVS  LTT F  
Sbjct: 241 LMHELLYYYMVRANPTWEFTWFFVIHGVCLALEIELKRAYEKKWQLQRAVSTLLTTMFTG 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  WLLFPLML++
Sbjct: 301 TTTLWLLFPLMLKT 314

BLAST of HG10022434 vs. ExPASy TrEMBL
Match: A0A5D3DHE7 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G00060 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.2e-143
Identity = 258/314 (82.17%), Postives = 283/314 (90.13%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M +EI+RFI VLPIFI+SSLYCYF+ASKFSK KLRLLSLLP+FS+F+V+PLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLLF+FD+GPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQE  PK
Sbjct: 61  SVGLFVTWLTTFKLLLFTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            L L+LPTKVVI AIL+LGN+YIDLVCPN  K WINCAT+YFYLDVVMSLSN FVR TFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNL+VSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYYI+RAIPTWE  WFFVIHGVCLA+EIELK+AY KRW+L  A S  LTTTF A
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  W LFP MLR+
Sbjct: 301 TTTLWFLFPPMLRT 314

BLAST of HG10022434 vs. ExPASy TrEMBL
Match: A0A1S3BIM7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490115 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.6e-143
Identity = 257/314 (81.85%), Postives = 282/314 (89.81%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M +EI+RFI VLPIFI+SSLYCYF+ASKFSK KLRLLSLLP+FS+F+V+PLYFSSILFTS
Sbjct: 1   MVAEIMRFIMVLPIFILSSLYCYFVASKFSKAKLRLLSLLPVFSIFLVLPLYFSSILFTS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           SV LFVTWLTTFKLLL +FD+GPLASNPPLSFPLFASIAFFPTR KHNKTTPHDQE  PK
Sbjct: 61  SVGLFVTWLTTFKLLLVTFDTGPLASNPPLSFPLFASIAFFPTRTKHNKTTPHDQESLPK 120

Query: 121 -LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
            L L+LPTKVVI AIL+LGN+YIDLVCPN  K WINCAT+YFYLDVVMSLSN FVR TFG
Sbjct: 121 LLHLDLPTKVVILAILILGNSYIDLVCPNYVKSWINCATMYFYLDVVMSLSNVFVRSTFG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIMVFVVSG 240
           +EVRQPFNEPYLATSLQNLWGRRWNL+VSE LH+TIYKP+RYKVG+PRW AV+ VFVVSG
Sbjct: 181 VEVRQPFNEPYLATSLQNLWGRRWNLIVSETLHSTIYKPIRYKVGMPRWVAVVTVFVVSG 240

Query: 241 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 300
           LMHELLYYYI+RAIPTWE  WFFVIHGVCLA+EIELK+AY KRW+L  A S  LTTTF A
Sbjct: 241 LMHELLYYYIVRAIPTWEFMWFFVIHGVCLALEIELKKAYEKRWQLQLAASTLLTTTFTA 300

Query: 301 TTAYWLLFPLMLRS 314
           TT  W LFP MLR+
Sbjct: 301 TTTLWFLFPPMLRT 314

BLAST of HG10022434 vs. ExPASy TrEMBL
Match: A0A6J1FEY7 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443337 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.2e-118
Identity = 217/319 (68.03%), Postives = 256/319 (80.25%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           MGSE+I F+KV+ I + S LYCYFIA K  KGK RL+S LP+FSLF+V+PLY SS   +S
Sbjct: 1   MGSELISFMKVILISMASLLYCYFIAPKLPKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQ----- 120
           S A  VTWLTTFKL+LFSF+ GPLA +  LSFPLFASIAF PTR+KH+KT  + Q     
Sbjct: 61  SFAFLVTWLTTFKLVLFSFNLGPLAFDSKLSFPLFASIAFLPTRIKHSKTAKYTQNQEEV 120

Query: 121 --EPPPKLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAF 180
             EP PK+PLNLP KVVI  +L++G  Y ++V P  AKL +NCATLYFYLD++M LSN F
Sbjct: 121 GKEPRPKIPLNLPAKVVIFVMLIMGKKYYEIVDP-IAKLCMNCATLYFYLDMIMCLSNVF 180

Query: 181 VRLTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIM 240
           VR  FG+EV +PF+EPYLATSLQN WGRRWN LVSE LHN +Y+PVRY+VG PRW AV++
Sbjct: 181 VRSRFGVEVSRPFDEPYLATSLQNFWGRRWNRLVSETLHNAVYQPVRYEVGGPRWMAVVV 240

Query: 241 VFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPL 300
           VFVVSGLMHELL YYIIRAIPTWE TWFFVIHG+C+A+EIELKRA GKRWR+HWAVSGPL
Sbjct: 241 VFVVSGLMHELLLYYIIRAIPTWEVTWFFVIHGLCVAVEIELKRALGKRWRIHWAVSGPL 300

Query: 301 TTTFMATTAYWLLFPLMLR 313
           T  F+  TA+WL FP +LR
Sbjct: 301 TMAFLVATAHWLFFPPLLR 318

BLAST of HG10022434 vs. ExPASy TrEMBL
Match: A0A6J1ILW9 (probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111476789 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.1e-117
Identity = 215/319 (67.40%), Postives = 258/319 (80.88%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           MGSE+I F+KVL I +VS LYCYFIA K SKGK RL+S LP+FSLF+V+PLY SS   +S
Sbjct: 1   MGSELISFMKVLLISMVSLLYCYFIAQKLSKGKFRLISFLPVFSLFLVLPLYVSSAFLSS 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQ----- 120
           +++ FVTWLTTFKL+LFSF+ GPLA +P LS  LF SIAF PTR+KHNKT  + Q     
Sbjct: 61  NISFFVTWLTTFKLILFSFNLGPLAFDPKLSLSLFVSIAFLPTRIKHNKTAKYVQNHDDL 120

Query: 121 --EPPPKLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAF 180
             EP PKLPL+LP KVVI A+LV+G  ++++  P  AKL++NCATLYFYLD++MSLSN F
Sbjct: 121 GNEPRPKLPLSLPAKVVIFAMLVMGGRHLEIGGP-IAKLYLNCATLYFYLDMIMSLSNVF 180

Query: 181 VRLTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRYKVGVPRWAAVIM 240
           VR  FG+EVR+PF+EPYLATSLQN WGRRWN  VSE LH  +Y+PVRY++GVPRW AV++
Sbjct: 181 VRSRFGVEVRRPFDEPYLATSLQNFWGRRWNQSVSETLHTAVYQPVRYEIGVPRWMAVVV 240

Query: 241 VFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPL 300
           VFVVSGLMHELL YYIIRAIPTWE TWFFV HG+C+A+EIELKRA GKRWR+HWAVS PL
Sbjct: 241 VFVVSGLMHELLLYYIIRAIPTWEITWFFVAHGLCVAVEIELKRALGKRWRIHWAVSRPL 300

Query: 301 TTTFMATTAYWLLFPLMLR 313
           T  F+  T +WL FP +LR
Sbjct: 301 TIAFLVATIHWLFFPPLLR 318

BLAST of HG10022434 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-54
Identity = 128/321 (39.88%), Postives = 184/321 (57.32%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+   IKV    I+S  YCY+I  +   G  R LS+ P+ +LF+V+PL+FSS+  + 
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
             A F+TWL  FKL+LFSFD GPL    P +FP F     FP +V+ N   P  Q   PK
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIP-IPTNFPRFFCFTCFPIKVQQN---PKSQNHLPK 120

Query: 121 LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGI 180
           L   +  K+ I A+L+   +Y   + P    L +    LY  ++++++     V ++ G 
Sbjct: 121 LVFAI--KLAIFAVLLHLYSYRQNLSP-TILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 181 EVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR------YKVGVPRWAAVIMV 240
           ++    N+PYLATSLQ+ WGRRWNL+V  IL   +Y P+R         G   +  ++  
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 241 FVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKR--AYGKRWRLHWAVSGP 300
           F+VSGL+HELL++Y+IR +PT E T FFV+HGVC A+E+ +K+     +RWRL   VS  
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRV 300

Query: 301 LTTTFMATTAYWLLFPLMLRS 314
           LT  F+  T  WL  P + RS
Sbjct: 301 LTVGFVFVTGGWLFTPQLKRS 314

BLAST of HG10022434 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.2e-54
Identity = 135/324 (41.67%), Postives = 185/324 (57.10%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  +    I+V    ++S  YCY+I+SK SKG LRLLS+LP+  LF+V+PL+ S + F +
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
              LF++WL  FKLLLF+FD GPL   PP     F   A  P +++ +   P     P  
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQD---PSPNAIPNL 120

Query: 121 LPLNLPTKVVICAILVLG-----NNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVR 180
            P  +P  V+   ILVLG       Y D + P    L + C  +Y  +++V+    A V 
Sbjct: 121 HPKPMPKWVLAVKILVLGVLLHVYEYRDGL-PRFVVLALYCLHIYLEVELVLVFVGAVVS 180

Query: 181 LTFGIEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR---YKVGVP---RWA 240
              G  +   FNEPYLATSLQ+ W RRWNL+VS +L +T++ PV+    ++  P    +A
Sbjct: 181 TLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFA 240

Query: 241 AVIMVFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAV 300
            V+  F VSGLMHELLY+Y+IR  PTWE T FFV+HG   A EI +KR    R   H AV
Sbjct: 241 GVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR-PPHRAV 300

Query: 301 SGPLTTTFMATTAYWLLFPLMLRS 314
           SG +  TF++ T  WL    +LR+
Sbjct: 301 SGLVVLTFVSVTGVWLFLAQVLRN 318

BLAST of HG10022434 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 208.8 bits (530), Expect = 6.5e-54
Identity = 118/309 (38.19%), Postives = 174/309 (56.31%), Query Frame = 0

Query: 8   FIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTSSVALFVT 67
           FIK   + I+S  Y +FIA    KG  RL+   P+F +F +VP    S+      A F+ 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 68  WLTTFKLLLFSFDSGPLASN-PPLSFPLFASIAFFPTRVK--HNKTTPHDQEPPPKLPLN 127
           WL  FKLLLF+   GPL+SN  PLS P+F +++  P +++     T  H  E   + PL 
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 128 LPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGIEVRQ 187
              K V   +++    Y     P    L +    +YF L+++++ + A VR    +E+  
Sbjct: 124 YTIKAVFVVLIIKAYEY-STKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 183

Query: 188 PFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKP--VRYKVGVPRWAAVIM---VFVVSG 247
            FN+PYLATSLQ+ WGRRWNL+V+ IL  T+Y+P    + V  P ++ ++     FVVSG
Sbjct: 184 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFSVLGPNYSQILAAFGTFVVSG 243

Query: 248 LMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRWRLHWAVSGPLTTTFMA 307
           +MHEL+++Y+ R  P W+  WFF+I+G C  +EI +K+    RWR   A+S  LT TF+ 
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 308 TTAYWLLFP 309
            TA WL  P
Sbjct: 304 VTALWLFLP 311

BLAST of HG10022434 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 208.4 bits (529), Expect = 8.5e-54
Identity = 132/322 (40.99%), Postives = 183/322 (56.83%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+   IKV    ++S  YCY+I+SK SKG LRLLSLLPIF +F+++PL+FSS+ F  
Sbjct: 1   MEEELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCV 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPH-DQEPPP 120
               F TWL  FKL LF+FD  PL S  P +   F   A FP ++  N ++     +P  
Sbjct: 61  ISGFFFTWLANFKLFLFAFDQEPL-SPLPSNLTRFFCFACFPIKINKNPSSNRIHNKPMS 120

Query: 121 KLPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFG 180
           K  L     +    + V  NNY D      A L +    +Y   ++++    A + +  G
Sbjct: 121 KWVLAFKLLIFSFLLHVYRNNY-DSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLLG 180

Query: 181 IEVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVRY----KVGVPR--WAAVIM 240
            E+   FN+PYLATSLQ  W RRWNL+V  +L   ++ PV+      +G+ R  +A ++ 
Sbjct: 181 CEMEPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGMLA 240

Query: 241 VFVVSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKRAYGKRW--RLHWAVSG 300
            F+VSGLMHEL+Y+Y+IR  PTWE T FF++HGV   +EI +KR    RW      AVSG
Sbjct: 241 TFIVSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMKR---MRWLPTPRRAVSG 300

Query: 301 PLTTTFMATTAYWLLFPLMLRS 314
              T F+  TA WL +P MLR+
Sbjct: 301 LAITVFLLVTAGWLFYPQMLRN 317

BLAST of HG10022434 vs. TAIR 10
Match: AT5G55330.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 206.5 bits (524), Expect = 3.2e-53
Identity = 123/318 (38.68%), Postives = 182/318 (57.23%), Query Frame = 0

Query: 1   MGSEIIRFIKVLPIFIVSSLYCYFIASKFSKGKLRLLSLLPIFSLFVVVPLYFSSILFTS 60
           M  E+  FI+V    I+S  YCY++  K     LRLLS+LP+  LF+++P++FS++  + 
Sbjct: 1   MEEELKLFIQVWVSAIISVTYCYYLTPKIKTSLLRLLSVLPVCVLFLIIPIFFSTVHSSF 60

Query: 61  SVALFVTWLTTFKLLLFSFDSGPLASNPPLSFPLFASIAFFPTRVKHNKTTPHDQEPPPK 120
           ++A F++ L   KL+LF+ + GPL   PP + P F   A FP +++  K  P +    PK
Sbjct: 61  TIAFFLSGLAVPKLILFALEKGPLFPLPP-NLPHFVCFACFPIKLQ-KKPNPENTNHFPK 120

Query: 121 LPLNLPTKVVICAILVLGNNYIDLVCPNNAKLWINCATLYFYLDVVMSLSNAFVRLTFGI 180
               L  KV I   L+L   +       N  L +    +Y  L++ ++L    V +T G 
Sbjct: 121 WVFAL--KVFIFGALLLQAYHYKQFLSTNFLLGLYALHIYLELEISLTLIKFLVSITLGC 180

Query: 181 EVRQPFNEPYLATSLQNLWGRRWNLLVSEILHNTIYKPVR-YKVGVPRW---AAVIMVFV 240
           ++   FNEPYLATSL + WG RWNL+VS+IL   +Y P+R ++     W    A+   F+
Sbjct: 181 DLEPQFNEPYLATSLHDFWGHRWNLMVSKILWLAVYNPIRQWRAKSSEWDRFFAIFATFL 240

Query: 241 VSGLMHELLYYYIIRAIPTWEATWFFVIHGVCLAMEIELKR--AYGKRWRLHWAVSGPLT 300
           VSG+ HE+LY+Y+ R  PTWE TWFFV+HG C+A E+ LKR     +RW ++ AVS  LT
Sbjct: 241 VSGVAHEILYFYLTREKPTWEVTWFFVLHGFCMAAEVALKRKTKLVQRWPVNPAVSRLLT 300

Query: 301 TTFMATTAYWLLFPLMLR 313
             F+  T  WL  P  +R
Sbjct: 301 VGFVFVTGVWLFSPQPIR 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897379.11.3e-14984.66LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 1 [Beni... [more]
XP_004140072.17.5e-14583.12probable long-chain-alcohol O-fatty-acyltransferase 6 [Cucumis sativus] >KGN4400... [more]
KAA0048128.12.4e-14382.17putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa]... [more]
XP_008447704.11.6e-14281.85PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
XP_038899290.12.4e-11969.02probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FJ761.8e-5339.88Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Q9FJ723.1e-5341.67Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9SV079.1e-5338.19Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Q9FJ731.2e-5240.99Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX... [more]
Q9FJ774.5e-5238.68Probable long-chain-alcohol O-fatty-acyltransferase 6 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A0A0K6W13.6e-14583.12MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G091220 PE=... [more]
A0A5D3DHE71.2e-14382.17Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw... [more]
A0A1S3BIM77.6e-14381.85probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1FEY72.2e-11868.03acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1ILW91.1e-11767.40probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT5G55340.11.3e-5439.88MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.12.2e-5441.67MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.16.5e-5438.19acyl-CoA sterol acyl transferase 1 [more]
AT5G55370.18.5e-5440.99MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55330.13.2e-5338.68MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 185..263
e-value: 2.3E-16
score: 59.8
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 1..312
NoneNo IPR availablePANTHERPTHR31595:SF8(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 1..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022434.1HG10022434.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity