HG10022431 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022431
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationChr05: 24184665 .. 24185717 (+)
RNA-Seq ExpressionHG10022431
SyntenyHG10022431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGCCGATCTCTCCGCCTTACTCAAAATCTCTCTCTCAGTCCTCTCCTCTTTACTCTACTCCTATTTCATATCCTCAAAGCTCCCAAAAGGCCTCCCAAGGCTCCTCTCTCTCCTTCCAATCCTCTCCCTCTTCACTCTCCTCCCTCTCCGCGTCTCCTCCATTTTCCTCGCCGGCATCGTCGCCTCCTTCATCACTTGGCTCGCTTCCTTCAAGCTCCTCCTCTTCTCCTTCGATTTAGGCCCTTTAGTTTCCGACCCACCATTTCCGTTTACTCTGTTCGCCTCACTCGCTTGTTTCCCAATCAGAATCAAGAAAAATCAGCTCAAACCAGATCCTCAAGATCCCCAAAAACCCATTGGTTCTCCTAAATTGCCTCTCAATCTTCCCGCCAAAGTTTTTTTGTTCGCCGTATTGGTCGCCGCCGAAGAATCTCTGCCTCCTTATTTGAAGCTCTGTTACCATTGTGTGTGTCTGTATTTTTTCATCGACGTTGTTGTGGGTTTTTTCAATACGATTACGAGATGGGGTTTTGGAATTGAACTTGAACCACCGTCGAATGAGCCGTATTTATCTACCTCGCTTCAGGATTTTTGGGGGAGGAGGTGGAATTTATTGGTTTCAAATATTTTGAGATTGACTGTTTTTTTGCCAGTTAGGACTGCCGTGATCGGCGTGGCGGGGCGGCAGCGGTCGGCGGCTGTGGCAGTGTTTGCGGTGTTTTTGGTGTCGGGATTGATGCACGAGCTGCTGTTTTATTATGTTAACCGAGCGGTTCCGAGTTGGGAGGTTACTGGGTTTTTTGTACTTCAAGGGGTTTGTGCGTTGGTGGAATTTCAGTTGAAGACGGCGGTGGGGGAAAAGTTCCGGCTGCATTGGGCGGTTTCTTGGCCGTTAACGATGGGGTTTATCGTTGTCACCGCCTCGTGGCTGTTCTTTCCGCCGGTATTGAGGAATGGGACGGTGGCCGGAATTATTGGGGAGTGTAGGGCGGCGTTGGAGTTGGTGAATGATCTGGTCATGTCCGTCTTTAAAAGACCGACTTGA

mRNA sequence

ATGGCGGCCGCCGATCTCTCCGCCTTACTCAAAATCTCTCTCTCAGTCCTCTCCTCTTTACTCTACTCCTATTTCATATCCTCAAAGCTCCCAAAAGGCCTCCCAAGGCTCCTCTCTCTCCTTCCAATCCTCTCCCTCTTCACTCTCCTCCCTCTCCGCGTCTCCTCCATTTTCCTCGCCGGCATCGTCGCCTCCTTCATCACTTGGCTCGCTTCCTTCAAGCTCCTCCTCTTCTCCTTCGATTTAGGCCCTTTAGTTTCCGACCCACCATTTCCGTTTACTCTGTTCGCCTCACTCGCTTGTTTCCCAATCAGAATCAAGAAAAATCAGCTCAAACCAGATCCTCAAGATCCCCAAAAACCCATTGGTTCTCCTAAATTGCCTCTCAATCTTCCCGCCAAAGTTTTTTTGTTCGCCGTATTGGTCGCCGCCGAAGAATCTCTGCCTCCTTATTTGAAGCTCTGTTACCATTGTGTGTGTCTGTATTTTTTCATCGACGTTGTTGTGGGTTTTTTCAATACGATTACGAGATGGGGTTTTGGAATTGAACTTGAACCACCGTCGAATGAGCCGTATTTATCTACCTCGCTTCAGGATTTTTGGGGGAGGAGGTGGAATTTATTGGTTTCAAATATTTTGAGATTGACTGTTTTTTTGCCAGTTAGGACTGCCGTGATCGGCGTGGCGGGGCGGCAGCGGTCGGCGGCTGTGGCAGTGTTTGCGGTGTTTTTGGTGTCGGGATTGATGCACGAGCTGCTGTTTTATTATGTTAACCGAGCGGTTCCGAGTTGGGAGGTTACTGGGTTTTTTGTACTTCAAGGGGTTTGTGCGTTGGTGGAATTTCAGTTGAAGACGGCGGTGGGGGAAAAGTTCCGGCTGCATTGGGCGGTTTCTTGGCCGTTAACGATGGGGTTTATCGTTGTCACCGCCTCGTGGCTGTTCTTTCCGCCGGTATTGAGGAATGGGACGGTGGCCGGAATTATTGGGGAGTGTAGGGCGGCGTTGGAGTTGGTGAATGATCTGGTCATGTCCGTCTTTAAAAGACCGACTTGA

Coding sequence (CDS)

ATGGCGGCCGCCGATCTCTCCGCCTTACTCAAAATCTCTCTCTCAGTCCTCTCCTCTTTACTCTACTCCTATTTCATATCCTCAAAGCTCCCAAAAGGCCTCCCAAGGCTCCTCTCTCTCCTTCCAATCCTCTCCCTCTTCACTCTCCTCCCTCTCCGCGTCTCCTCCATTTTCCTCGCCGGCATCGTCGCCTCCTTCATCACTTGGCTCGCTTCCTTCAAGCTCCTCCTCTTCTCCTTCGATTTAGGCCCTTTAGTTTCCGACCCACCATTTCCGTTTACTCTGTTCGCCTCACTCGCTTGTTTCCCAATCAGAATCAAGAAAAATCAGCTCAAACCAGATCCTCAAGATCCCCAAAAACCCATTGGTTCTCCTAAATTGCCTCTCAATCTTCCCGCCAAAGTTTTTTTGTTCGCCGTATTGGTCGCCGCCGAAGAATCTCTGCCTCCTTATTTGAAGCTCTGTTACCATTGTGTGTGTCTGTATTTTTTCATCGACGTTGTTGTGGGTTTTTTCAATACGATTACGAGATGGGGTTTTGGAATTGAACTTGAACCACCGTCGAATGAGCCGTATTTATCTACCTCGCTTCAGGATTTTTGGGGGAGGAGGTGGAATTTATTGGTTTCAAATATTTTGAGATTGACTGTTTTTTTGCCAGTTAGGACTGCCGTGATCGGCGTGGCGGGGCGGCAGCGGTCGGCGGCTGTGGCAGTGTTTGCGGTGTTTTTGGTGTCGGGATTGATGCACGAGCTGCTGTTTTATTATGTTAACCGAGCGGTTCCGAGTTGGGAGGTTACTGGGTTTTTTGTACTTCAAGGGGTTTGTGCGTTGGTGGAATTTCAGTTGAAGACGGCGGTGGGGGAAAAGTTCCGGCTGCATTGGGCGGTTTCTTGGCCGTTAACGATGGGGTTTATCGTTGTCACCGCCTCGTGGCTGTTCTTTCCGCCGGTATTGAGGAATGGGACGGTGGCCGGAATTATTGGGGAGTGTAGGGCGGCGTTGGAGTTGGTGAATGATCTGGTCATGTCCGTCTTTAAAAGACCGACTTGA

Protein sequence

MAAADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGSPKLPLNLPAKVFLFAVLVAAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELVNDLVMSVFKRPT
Homology
BLAST of HG10022431 vs. NCBI nr
Match: XP_038897084.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 558.5 bits (1438), Expect = 4.0e-155
Identity = 284/348 (81.61%), Postives = 307/348 (88.22%), Query Frame = 0

Query: 3   AADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGI 62
           A DLSALLKISLSVL+SLLYSYFISSKLPKGLPRLLSLLPIL LFTLLPL VSSI  AGI
Sbjct: 4   AGDLSALLKISLSVLASLLYSYFISSKLPKGLPRLLSLLPILYLFTLLPLLVSSILFAGI 63

Query: 63  VASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPI 122
           VASFITWLASFKLLLFSFDLGPLVS+PPF F LFASL CFP+R+K+NQ  P P++P KPI
Sbjct: 64  VASFITWLASFKLLLFSFDLGPLVSEPPFSFPLFASLVCFPVRMKQNQPNPVPENPPKPI 123

Query: 123 GSPKLPLNLPAKVFLFAVLVAAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGI 182
            SPKLPLNLP KV LFA+L  A+E+LP YLKLCYHC  LYFFIDVVVGFFNTI RW FGI
Sbjct: 124 DSPKLPLNLPTKVLLFAILTVADEALPRYLKLCYHCAALYFFIDVVVGFFNTIVRWSFGI 183

Query: 183 ELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAV 242
           ELE PSNEPYL+TSLQDFWGRRWN+LVSNIL+LTVFLPVR+A+I   GR+RSAAVAVFAV
Sbjct: 184 ELEEPSNEPYLATSLQDFWGRRWNILVSNILKLTVFLPVRSAMISRTGRRRSAAVAVFAV 243

Query: 243 FLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLT 302
           F+VSGLMHELLFYYVNR  PSWEVT FFVLQG+C LVEFQLKTAVG+KFRLHWAVS P T
Sbjct: 244 FVVSGLMHELLFYYVNRVAPSWEVTAFFVLQGICVLVEFQLKTAVGDKFRLHWAVSLPFT 303

Query: 303 MGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELVNDLVMSVFKRPT 351
           +GF V TASWLFFPPVLRNGTV G+I ECRA LEL    VMSVF+RP+
Sbjct: 304 LGFTVTTASWLFFPPVLRNGTVVGLIRECRAGLELAIVPVMSVFRRPS 351

BLAST of HG10022431 vs. NCBI nr
Match: XP_004151329.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN44056.1 hypothetical protein Csa_015438 [Cucumis sativus])

HSP 1 Score: 530.8 bits (1366), Expect = 8.9e-147
Identity = 274/344 (79.65%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 4   ADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 63
           ADL ALLKI LSVL+SLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPL VSSI  A IV
Sbjct: 6   ADLFALLKIFLSVLASLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLLVSSILFAAIV 65

Query: 64  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIG 123
           +S +TWL SFKLLLFSF  GPLVSDPP PF LF SLA FPIRIKK       ++ QKPI 
Sbjct: 66  SSIVTWLTSFKLLLFSFHSGPLVSDPPLPFLLFVSLAFFPIRIKK-------KESQKPID 125

Query: 124 SPKLPLNLPAKVFLFAVLVAAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGIE 183
           SPKLP+N PAKVFLF VL+A E+SLPPY+KLC++CV LYFFID+VVGFFNTI RW FGIE
Sbjct: 126 SPKLPINFPAKVFLFTVLMAVEDSLPPYMKLCFNCVALYFFIDIVVGFFNTIARWVFGIE 185

Query: 184 LEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAVF 243
            E PSNEPYLSTSLQDFWGRRWN+LVSN+LRLTVF+PVR+A+I VAGR+RSAAV VFAVF
Sbjct: 186 FEEPSNEPYLSTSLQDFWGRRWNILVSNLLRLTVFIPVRSAMIDVAGRRRSAAVGVFAVF 245

Query: 244 LVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLTM 303
           +VSGLMHELLFYYVNR  PSWEVTGFFVLQGVC L+EFQLK AVGEKFR+H+AV W LTM
Sbjct: 246 VVSGLMHELLFYYVNRESPSWEVTGFFVLQGVCVLLEFQLKMAVGEKFRMHYAVCWVLTM 305

Query: 304 GFIVVTASWLFFPPVLRNGTVAGIIGECRAALELVNDLVMSVFK 348
           GF+VVTASWLFFPPVLRNGTVA + G+CRA LELVNDLV  V K
Sbjct: 306 GFVVVTASWLFFPPVLRNGTVARLTGDCRAVLELVNDLVKFVLK 342

BLAST of HG10022431 vs. NCBI nr
Match: XP_022981530.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 484.2 bits (1245), Expect = 9.6e-133
Identity = 248/342 (72.51%), Postives = 285/342 (83.33%), Query Frame = 0

Query: 3   AADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGI 62
           AADL  LLKIS +VL+SLLYSYFISSKLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 63  VASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP- 122
           VA FITWLASFKLLLF FD GPL SDPP  F LF S+AC+PIRIK+NQ  PDPQ+PQKP 
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKPT 121

Query: 123 ------IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFF 182
                 I SPKLPLNLPAK FLFAVLVA +   ++L P   LC++CV LY FIDVV+G  
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGIC 181

Query: 183 NTITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQ 242
           NT  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGRR 241

Query: 243 RSAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFR 302
           R+A VAVFA F+VSGLMHEL+FYYV  A+P+WEVTGFFVL GVC +VEF +K A+GE +R
Sbjct: 242 RAAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENWR 301

Query: 303 LHWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAA 335
           LHWAV+ PLT+ F+V+TA+WLFFPP+L+ G VA +IGEC+AA
Sbjct: 302 LHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAA 343

BLAST of HG10022431 vs. NCBI nr
Match: XP_023525214.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.6e-132
Identity = 248/342 (72.51%), Postives = 285/342 (83.33%), Query Frame = 0

Query: 3   AADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGI 62
           AADL  LLKIS +VL+SLLYSYFIS+KLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISTKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 63  VASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP- 122
           VA FITWLASFKLLLF FD GPL +DPP  F LF S+ACFPIRIK+NQ  PDPQ+PQKP 
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFTDPPLSFPLFVSIACFPIRIKQNQPDPDPQNPQKPT 121

Query: 123 ------IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFF 182
                 I SPKLPLNLPAK FLFAVLVA +   ++L P   LC++CV LY FIDVV+G  
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYLFIDVVLGLS 181

Query: 183 NTITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQ 242
           NT  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRR 241

Query: 243 RSAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFR 302
           R+A VAVFA F+VSGLMHELLFYYV  AVP+WEVTGFFVL GVC ++EF +K A+GE +R
Sbjct: 242 RAAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVLEFWVKKALGENWR 301

Query: 303 LHWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAA 335
           LHWAV+ PLT+ F+V+TA+WLFFPP+L+ G VA +IGEC+AA
Sbjct: 302 LHWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAVIGECKAA 343

BLAST of HG10022431 vs. NCBI nr
Match: XP_022940347.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 480.7 bits (1236), Expect = 1.1e-131
Identity = 248/341 (72.73%), Postives = 283/341 (82.99%), Query Frame = 0

Query: 4   ADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 63
           ADL  LLKIS +VL+SLLYSYFISSKLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 64  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP-- 123
           A FITWLASFKLLLF FD GPL SDPP  F LF S++CFPIRIK+NQ  PDPQ+PQKP  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 124 -----IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFFN 183
                I SPKL LNLPAK FLFAVLVA +   ++L P   LC++CV LYFFIDV++G  N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 184 TITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQR 243
           T  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+R
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 244 SAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRL 303
           +A VAVFA F+VSGLMHELLFYYV  AVP+WEVTGFFVL GVC +VEF +K  +GE +RL
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWRL 301

Query: 304 HWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAA 335
           HWAV+ PLT+ F+V+TA+WLFFPP+L+ G VA IIGEC+AA
Sbjct: 302 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAA 342

BLAST of HG10022431 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.4e-62
Identity = 147/343 (42.86%), Postives = 212/343 (61.81%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVA 64
           +L  L+K+ +S + S+ Y Y+I  ++  G PR LS+ P+L+LF +LPL  SS+ L+ I A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 65  SFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGS 124
            F+TWLA+FKL+LFSFD GPL+  P   F  F    CFPI++++N        P+     
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIPIPT-NFPRFFCFTCFPIKVQQN--------PKSQNHL 123

Query: 125 PKLPLNLPAKVFLFAVLV---AAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFG 184
           PKL   +  K+ +FAVL+   +  ++L P + L  + V LY  I++++ F   +     G
Sbjct: 124 PKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLG 183

Query: 185 IELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVF- 244
            +LEP SN+PYL+TSLQDFWGRRWNL+V  ILR  V+ P+R     V+ R+ S+  A+F 
Sbjct: 184 CDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRR----VSERKMSSGWALFP 243

Query: 245 ---AVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQL--KTAVGEKFRLHW 304
              A F+VSGL+HELLF+Y+ R +P+ EVT FFVL GVC  VE  +  KT V +++RL  
Sbjct: 244 GILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSP 303

Query: 305 AVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELV 339
            VS  LT+GF+ VT  WLF P + R+G +     E    +E +
Sbjct: 304 GVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFI 331

BLAST of HG10022431 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 8.1e-58
Identity = 139/332 (41.87%), Postives = 190/332 (57.23%), Query Frame = 0

Query: 9   LLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVASFIT 68
           L+++ +S L SL Y Y+ISSKL KG+ RLLS+LP+  LF +LPL +S +    I   F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 69  WLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGSPKLP 128
           WLA+FKLLLF+FD GPL   PP   + F   AC PI+I+++   P P         P   
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQD---PSPNAIPNLHPKPMPK 127

Query: 129 LNLPAKVFLFAVLVAAEE---SLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGIELE 188
             L  K+ +  VL+   E    LP ++ L  +C+ +Y  +++V+ F   +     G  +E
Sbjct: 128 WVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIE 187

Query: 189 PPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAVFLV 248
           P  NEPYL+TSLQDFW RRWNL+VS +LR TV +PV+     +     +    V A F V
Sbjct: 188 PVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFV 247

Query: 249 SGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLTMGF 308
           SGLMHELL++Y+ R  P+WEVT FFVL G     E  +K     +   H AVS  + + F
Sbjct: 248 SGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR-PPHRAVSGLVVLTF 307

Query: 309 IVVTASWLFFPPVLRNGTVAGIIGECRAALEL 338
           + VT  WLF   VLRN      IGEC   L+L
Sbjct: 308 VSVTGVWLFLAQVLRNNVHEKAIGECLLVLDL 334

BLAST of HG10022431 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.8e-57
Identity = 138/341 (40.47%), Postives = 207/341 (60.70%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVA 64
           +L   +K+ +S + S+ Y Y++S+ +  G+ RLLS+LP+ +LF + PL  S +  +G +A
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 65  SFITWLASFKLLLFSFDLGPLVSDPPFPFTL--FASLACFPIRIKKNQLKPDPQDPQKPI 124
            F++WLA+FKL+LFSFD GPL    P P TL  F  + CFPI+ ++N   P+ Q+ + PI
Sbjct: 64  FFLSWLANFKLILFSFDQGPL---SPLPRTLSRFICITCFPIKPQQN---PNIQNYKIPI 123

Query: 125 GSPKLPLNLPAKVFLFAVLVAA---EESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWG 184
                      KV +F VL+     ++ L P L L ++ + ++  +++V      +    
Sbjct: 124 WL------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFIT 183

Query: 185 FGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAV 244
            G +LEP SNEPYL+TSLQDFWGRRWNL+V  ILR  V+LP R          ++  + V
Sbjct: 184 LGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGV 243

Query: 245 FAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLK--TAVGEKFRLHWAV 304
           FA FLVSG +HE+LF+Y+ R VP+ EVT FF+L GVC + E  +K  T V   +R+   V
Sbjct: 244 FAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTV 303

Query: 305 SWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELV 339
           S  LT+GF+VVT+ W FF P++R+G +  +  E    ++ V
Sbjct: 304 SRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFV 331

BLAST of HG10022431 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.9e-57
Identity = 136/330 (41.21%), Postives = 189/330 (57.27%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPK-GLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 64
           +L    ++ +S +++  Y  F+ +  P  G  RLL LLP++ LF  LPLR+SS  L G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 65  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIG 124
           A ++ WLA+FKLLLF+F LGPL S+P      F S    PI+ + +      ++ +    
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLSF 125

Query: 125 SPKLPLNLPAKVFLFAVLVAAEE---SLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGF 184
             +  +   AK+  FA ++   E    LP ++    +C   Y   ++ +     I R   
Sbjct: 126 EWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARATL 185

Query: 185 GIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVF 244
           G++L P  NEPYL+TSLQDFWGRRWNL+VS+IL LT + PVR  +      +   A A+ 
Sbjct: 186 GLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAML 245

Query: 245 AVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWP 304
             F VSGLMHE+ F+Y+ RA PSWEVTGFFVL GVC  VE  +K AV  K RL   VS  
Sbjct: 246 VAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSGA 305

Query: 305 LTMGFIVVTASWLFFPPVLRNGTVAGIIGE 331
           LT+GF++VT  WLF P ++R+G     I E
Sbjct: 306 LTVGFVMVTGGWLFLPQLVRHGVDLKTIDE 334

BLAST of HG10022431 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.4e-56
Identity = 128/332 (38.55%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 6   LSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVAS 65
           +++ +K    V+ SL Y++FI+  +PKG+ RL+   P+  +F ++P  + S+ L GI A 
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 66  FITWLASFKLLLFSFDLGPLVSD-PPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGS 125
           FI WLA+FKLLLF+   GPL S+  P    +F +++C PI+I   QL P P       GS
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI---QLSPKPTKTHSHEGS 120

Query: 126 PKLPLNLPAKVFLFAVLVAAEE---SLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFG 185
            + PL    K     +++ A E    LP  + L  + + +YF +++++       R    
Sbjct: 121 TEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 180

Query: 186 IELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFA 245
           +ELEP  N+PYL+TSLQDFWGRRWNL+V+ ILR TV+ P    +  V G   S  +A F 
Sbjct: 181 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEP-SLQLFSVLGPNYSQILAAFG 240

Query: 246 VFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPL 305
            F+VSG+MHEL+F+Y+ R  P W++  FF++ G C  VE  +K  +  ++R   A+S  L
Sbjct: 241 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 300

Query: 306 TMGFIVVTASWLFFPPVLRNGTVAGIIGECRA 334
           T+ F++VTA WLF P   R   V   + E  A
Sbjct: 301 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAA 328

BLAST of HG10022431 vs. ExPASy TrEMBL
Match: A0A0A0K8E9 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=3 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 4.3e-147
Identity = 274/344 (79.65%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 4   ADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 63
           ADL ALLKI LSVL+SLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPL VSSI  A IV
Sbjct: 6   ADLFALLKIFLSVLASLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLLVSSILFAAIV 65

Query: 64  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIG 123
           +S +TWL SFKLLLFSF  GPLVSDPP PF LF SLA FPIRIKK       ++ QKPI 
Sbjct: 66  SSIVTWLTSFKLLLFSFHSGPLVSDPPLPFLLFVSLAFFPIRIKK-------KESQKPID 125

Query: 124 SPKLPLNLPAKVFLFAVLVAAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGIE 183
           SPKLP+N PAKVFLF VL+A E+SLPPY+KLC++CV LYFFID+VVGFFNTI RW FGIE
Sbjct: 126 SPKLPINFPAKVFLFTVLMAVEDSLPPYMKLCFNCVALYFFIDIVVGFFNTIARWVFGIE 185

Query: 184 LEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAVF 243
            E PSNEPYLSTSLQDFWGRRWN+LVSN+LRLTVF+PVR+A+I VAGR+RSAAV VFAVF
Sbjct: 186 FEEPSNEPYLSTSLQDFWGRRWNILVSNLLRLTVFIPVRSAMIDVAGRRRSAAVGVFAVF 245

Query: 244 LVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLTM 303
           +VSGLMHELLFYYVNR  PSWEVTGFFVLQGVC L+EFQLK AVGEKFR+H+AV W LTM
Sbjct: 246 VVSGLMHELLFYYVNRESPSWEVTGFFVLQGVCVLLEFQLKMAVGEKFRMHYAVCWVLTM 305

Query: 304 GFIVVTASWLFFPPVLRNGTVAGIIGECRAALELVNDLVMSVFK 348
           GF+VVTASWLFFPPVLRNGTVA + G+CRA LELVNDLV  V K
Sbjct: 306 GFVVVTASWLFFPPVLRNGTVARLTGDCRAVLELVNDLVKFVLK 342

BLAST of HG10022431 vs. ExPASy TrEMBL
Match: A0A6J1IZR7 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480619 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.7e-133
Identity = 248/342 (72.51%), Postives = 285/342 (83.33%), Query Frame = 0

Query: 3   AADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGI 62
           AADL  LLKIS +VL+SLLYSYFISSKLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGI
Sbjct: 2   AADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGI 61

Query: 63  VASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP- 122
           VA FITWLASFKLLLF FD GPL SDPP  F LF S+AC+PIRIK+NQ  PDPQ+PQKP 
Sbjct: 62  VAFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSIACYPIRIKQNQYDPDPQNPQKPT 121

Query: 123 ------IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFF 182
                 I SPKLPLNLPAK FLFAVLVA +   ++L P   LC++CV LY FIDVV+G  
Sbjct: 122 DPPLKTIDSPKLPLNLPAKGFLFAVLVAGDDFAQNLSPNTMLCFYCVSLYLFIDVVLGIC 181

Query: 183 NTITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQ 242
           NT  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+
Sbjct: 182 NTFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVVGRR 241

Query: 243 RSAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFR 302
           R+A VAVFA F+VSGLMHEL+FYYV  A+P+WEVTGFFVL GVC +VEF +K A+GE +R
Sbjct: 242 RAAVVAVFATFVVSGLMHELVFYYVTGAIPTWEVTGFFVLHGVCVVVEFWVKMALGENWR 301

Query: 303 LHWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAA 335
           LHWAV+ PLT+ F+V+TA+WLFFPP+L+ G VA +IGEC+AA
Sbjct: 302 LHWAVAGPLTLAFVVITAAWLFFPPLLKTGVVAAVIGECKAA 343

BLAST of HG10022431 vs. ExPASy TrEMBL
Match: A0A6J1FJC3 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 5.1e-132
Identity = 248/341 (72.73%), Postives = 283/341 (82.99%), Query Frame = 0

Query: 4   ADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 63
           ADL  LLKIS +VL+SLLYSYFISSKLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 64  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP-- 123
           A FITWLASFKLLLF FD GPL SDPP  F LF S++CFPIRIK+NQ  PDPQ+PQKP  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 124 -----IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFFN 183
                I SPKL LNLPAK FLFAVLVA +   ++L P   LC++CV LYFFIDV++G  N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 184 TITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQR 243
           T  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+R
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 244 SAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRL 303
           +A VAVFA F+VSGLMHELLFYYV  AVP+WEVTGFFVL GVC +VEF +K  +GE +RL
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVKMVLGENWRL 301

Query: 304 HWAVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAA 335
           HWAV+ PLT+ F+V+TA+WLFFPP+L+ G VA IIGEC+AA
Sbjct: 302 HWAVAGPLTLAFVVITAAWLFFPPLLKTGAVAAIIGECKAA 342

BLAST of HG10022431 vs. ExPASy TrEMBL
Match: A0A6J1CCA4 (LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Momordica charantia OX=3673 GN=LOC111010365 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.8e-116
Identity = 234/348 (67.24%), Postives = 266/348 (76.44%), Query Frame = 0

Query: 3   AADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGI 62
           AA+L AL+K+SL V +SLLYSYF++SKLPKG  RLLSLLPI  LF LLP       L+GI
Sbjct: 2   AAELHALIKVSLCVFASLLYSYFVASKLPKGKFRLLSLLPIFPLFALLP-----XLLSGI 61

Query: 63  VASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPI 122
           VA FITWLASFKLL FSFD GPL SDPP PF LFAS+ACFPIRIK+N  K     P  P 
Sbjct: 62  VAFFITWLASFKLLFFSFDSGPLASDPPLPFPLFASIACFPIRIKQNPPKTLHPPPHLP- 121

Query: 123 GSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWG 182
            SPKLPLNLPAK  LFA LVAA+   +SLP   KLC++C  LY FIDVV+G  N I R  
Sbjct: 122 DSPKLPLNLPAKALLFAALVAADDFAQSLPQNAKLCFYCAILYPFIDVVLGLSNAIVRSA 181

Query: 183 FGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAA-VA 242
           FGIELE PSNEPYL+TSLQDFWGRRWNLLVSN LR  V+ PVR A+    GR+R AA  A
Sbjct: 182 FGIELEQPSNEPYLATSLQDFWGRRWNLLVSNTLRYXVYRPVRAAL----GRRRGAAPAA 241

Query: 243 VFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVS 302
           VFA F+VSGLMHELLFYYV RAVP+WEVT FFVL GVC +VEF++K AVGEK+RLHWAVS
Sbjct: 242 VFATFVVSGLMHELLFYYVTRAVPAWEVTAFFVLHGVCVVVEFRVKRAVGEKWRLHWAVS 301

Query: 303 WPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELVNDLVMSVF 347
            PLT+ F+VVT +WLFFPP+LR G V G++ EC++A E V   V SVF
Sbjct: 302 GPLTVAFVVVTGAWLFFPPLLRTGAVVGVLRECKSAAEFVKAQVSSVF 339

BLAST of HG10022431 vs. ExPASy TrEMBL
Match: A0A6J1FP14 (probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445989 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 216/291 (74.23%), Postives = 241/291 (82.82%), Query Frame = 0

Query: 4   ADLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIV 63
           ADL  LLKIS +VL+SLLYSYFISSKLPKGLPRLLSLLPILSL+TLLPL +SS+ LAGIV
Sbjct: 2   ADLYTLLKISAAVLASLLYSYFISSKLPKGLPRLLSLLPILSLYTLLPLPLSSLLLAGIV 61

Query: 64  ASFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKP-- 123
           A FITWLASFKLLLF FD GPL SDPP  F LF S++CFPIRIK+NQ  PDPQ+PQKP  
Sbjct: 62  AFFITWLASFKLLLFCFDSGPLFSDPPLSFPLFVSISCFPIRIKQNQPDPDPQNPQKPTD 121

Query: 124 -----IGSPKLPLNLPAKVFLFAVLVAAE---ESLPPYLKLCYHCVCLYFFIDVVVGFFN 183
                I SPKL LNLPAK FLFAVLVA +   ++L P   LC++CV LYFFIDV++G  N
Sbjct: 122 PLLKTIDSPKLRLNLPAKGFLFAVLVAGDDFAQNLSPNAMLCFYCVSLYFFIDVILGLCN 181

Query: 184 TITRWGFGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQR 243
           T  RWGFGIELEPPSNEPYL+TSL+DFWGRRWNLLVSN LR +VFLPVR AV GV GR+R
Sbjct: 182 TFVRWGFGIELEPPSNEPYLATSLRDFWGRRWNLLVSNTLRWSVFLPVRAAVSGVLGRRR 241

Query: 244 SAAVAVFAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLK 285
           +A VAVFA F+VSGLMHELLFYYV  AVP+WEVTGFFVL GVC +VEF +K
Sbjct: 242 AAVVAVFATFVVSGLMHELLFYYVTGAVPTWEVTGFFVLHGVCVVVEFWVK 292

BLAST of HG10022431 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.8e-63
Identity = 147/343 (42.86%), Postives = 212/343 (61.81%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVA 64
           +L  L+K+ +S + S+ Y Y+I  ++  G PR LS+ P+L+LF +LPL  SS+ L+ I A
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 65  SFITWLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGS 124
            F+TWLA+FKL+LFSFD GPL+  P   F  F    CFPI++++N        P+     
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIPIPT-NFPRFFCFTCFPIKVQQN--------PKSQNHL 123

Query: 125 PKLPLNLPAKVFLFAVLV---AAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFG 184
           PKL   +  K+ +FAVL+   +  ++L P + L  + V LY  I++++ F   +     G
Sbjct: 124 PKLVFAI--KLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLG 183

Query: 185 IELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVF- 244
            +LEP SN+PYL+TSLQDFWGRRWNL+V  ILR  V+ P+R     V+ R+ S+  A+F 
Sbjct: 184 CDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRR----VSERKMSSGWALFP 243

Query: 245 ---AVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQL--KTAVGEKFRLHW 304
              A F+VSGL+HELLF+Y+ R +P+ EVT FFVL GVC  VE  +  KT V +++RL  
Sbjct: 244 GILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSP 303

Query: 305 AVSWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELV 339
            VS  LT+GF+ VT  WLF P + R+G +     E    +E +
Sbjct: 304 GVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFI 331

BLAST of HG10022431 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 225.7 bits (574), Expect = 5.7e-59
Identity = 139/332 (41.87%), Postives = 190/332 (57.23%), Query Frame = 0

Query: 9   LLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVASFIT 68
           L+++ +S L SL Y Y+ISSKL KG+ RLLS+LP+  LF +LPL +S +    I   F++
Sbjct: 8   LIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISVLFLS 67

Query: 69  WLASFKLLLFSFDLGPLVSDPPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGSPKLP 128
           WLA+FKLLLF+FD GPL   PP   + F   AC PI+I+++   P P         P   
Sbjct: 68  WLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQD---PSPNAIPNLHPKPMPK 127

Query: 129 LNLPAKVFLFAVLVAAEE---SLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFGIELE 188
             L  K+ +  VL+   E    LP ++ L  +C+ +Y  +++V+ F   +     G  +E
Sbjct: 128 WVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNIE 187

Query: 189 PPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFAVFLV 248
           P  NEPYL+TSLQDFW RRWNL+VS +LR TV +PV+     +     +    V A F V
Sbjct: 188 PVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFFV 247

Query: 249 SGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPLTMGF 308
           SGLMHELL++Y+ R  P+WEVT FFVL G     E  +K     +   H AVS  + + F
Sbjct: 248 SGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLR-PPHRAVSGLVVLTF 307

Query: 309 IVVTASWLFFPPVLRNGTVAGIIGECRAALEL 338
           + VT  WLF   VLRN      IGEC   L+L
Sbjct: 308 VSVTGVWLFLAQVLRNNVHEKAIGECLLVLDL 334

BLAST of HG10022431 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-58
Identity = 138/341 (40.47%), Postives = 207/341 (60.70%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVA 64
           +L   +K+ +S + S+ Y Y++S+ +  G+ RLLS+LP+ +LF + PL  S +  +G +A
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 65  SFITWLASFKLLLFSFDLGPLVSDPPFPFTL--FASLACFPIRIKKNQLKPDPQDPQKPI 124
            F++WLA+FKL+LFSFD GPL    P P TL  F  + CFPI+ ++N   P+ Q+ + PI
Sbjct: 64  FFLSWLANFKLILFSFDQGPL---SPLPRTLSRFICITCFPIKPQQN---PNIQNYKIPI 123

Query: 125 GSPKLPLNLPAKVFLFAVLVAA---EESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWG 184
                      KV +F VL+     ++ L P L L ++ + ++  +++V      +    
Sbjct: 124 WL------FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFIT 183

Query: 185 FGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAV 244
            G +LEP SNEPYL+TSLQDFWGRRWNL+V  ILR  V+LP R          ++  + V
Sbjct: 184 LGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGV 243

Query: 245 FAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLK--TAVGEKFRLHWAV 304
           FA FLVSG +HE+LF+Y+ R VP+ EVT FF+L GVC + E  +K  T V   +R+   V
Sbjct: 244 FAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTV 303

Query: 305 SWPLTMGFIVVTASWLFFPPVLRNGTVAGIIGECRAALELV 339
           S  LT+GF+VVT+ W FF P++R+G +  +  E    ++ V
Sbjct: 304 SRLLTVGFVVVTSGWFFF-PLIRSGIIERLASEALMCIDFV 331

BLAST of HG10022431 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 219.9 bits (559), Expect = 3.1e-57
Identity = 128/332 (38.55%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 6   LSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVAS 65
           +++ +K    V+ SL Y++FI+  +PKG+ RL+   P+  +F ++P  + S+ L GI A 
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 66  FITWLASFKLLLFSFDLGPLVSD-PPFPFTLFASLACFPIRIKKNQLKPDPQDPQKPIGS 125
           FI WLA+FKLLLF+   GPL S+  P    +F +++C PI+I   QL P P       GS
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI---QLSPKPTKTHSHEGS 120

Query: 126 PKLPLNLPAKVFLFAVLVAAEE---SLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWGFG 185
            + PL    K     +++ A E    LP  + L  + + +YF +++++       R    
Sbjct: 121 TEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSD 180

Query: 186 IELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAVFA 245
           +ELEP  N+PYL+TSLQDFWGRRWNL+V+ ILR TV+ P    +  V G   S  +A F 
Sbjct: 181 LELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEP-SLQLFSVLGPNYSQILAAFG 240

Query: 246 VFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLKTAVGEKFRLHWAVSWPL 305
            F+VSG+MHEL+F+Y+ R  P W++  FF++ G C  VE  +K  +  ++R   A+S  L
Sbjct: 241 TFVVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVL 300

Query: 306 TMGFIVVTASWLFFPPVLRNGTVAGIIGECRA 334
           T+ F++VTA WLF P   R   V   + E  A
Sbjct: 301 TLTFVMVTALWLFLPEFNRCNIVEKALDEYAA 328

BLAST of HG10022431 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-56
Identity = 134/332 (40.36%), Postives = 197/332 (59.34%), Query Frame = 0

Query: 5   DLSALLKISLSVLSSLLYSYFISSKLPKGLPRLLSLLPILSLFTLLPLRVSSIFLAGIVA 64
           +L +L+K+ +  + S+ Y Y+ S+++  G+ RLLS+LP+  LF +LPL VSS+  +G  A
Sbjct: 4   ELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTA 63

Query: 65  SFITWLASFKLLLFSFDLGPLVSDPPFPFTL--FASLACFPIRIKKNQLKPDPQDPQKPI 124
            F++WLA+FKL+LFSFD GPL    P P  L  F    CFPI++++N   P PQ+     
Sbjct: 64  FFLSWLANFKLILFSFDQGPLF---PVPSNLSRFVCFTCFPIKLQQN---PKPQNQMPKW 123

Query: 125 GSPKLPLNLPAKVFLFAVLV---AAEESLPPYLKLCYHCVCLYFFIDVVVGFFNTITRWG 184
           G          K+  F VL+     ++ + P + L  + + +Y   ++++     +    
Sbjct: 124 G-------FAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSIS 183

Query: 185 FGIELEPPSNEPYLSTSLQDFWGRRWNLLVSNILRLTVFLPVRTAVIGVAGRQRSAAVAV 244
              +LEP  NEPYLSTSLQDFWGRRWNL+V  ILR  V+LPVR          ++  + V
Sbjct: 184 LWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGV 243

Query: 245 FAVFLVSGLMHELLFYYVNRAVPSWEVTGFFVLQGVCALVEFQLK-TAVGEKFRLHWAVS 304
           FA FLVSG++HEL+F+Y  R  P+ EVT FFVL GVC   E   K T +  ++++   VS
Sbjct: 244 FASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVS 303

Query: 305 WPLTMGFIVVTASWLFFPPVLRNGTVAGIIGE 331
             LT+GF+V+T  WLFFP + R+G +  +  E
Sbjct: 304 RLLTVGFVVMTGGWLFFPHLARSGMIERLADE 322

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897084.14.0e-15581.61probable long-chain-alcohol O-fatty-acyltransferase 5 [Benincasa hispida][more]
XP_004151329.18.9e-14779.65probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis sativus] >KGN4405... [more]
XP_022981530.19.6e-13372.51probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima][more]
XP_023525214.13.6e-13272.51probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep... [more]
XP_022940347.11.1e-13172.73probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
Q9FJ765.4e-6242.86Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Q9FJ728.1e-5841.87Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9FJ741.8e-5740.47Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX... [more]
Q9XGY68.9e-5741.21Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ... [more]
Q9SV074.4e-5638.55Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Match NameE-valueIdentityDescription
A0A0A0K8E94.3e-14779.65MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G145990 PE=... [more]
A0A6J1IZR74.7e-13372.51probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366... [more]
A0A6J1FJC35.1e-13272.73probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X1 OS=Cucurbita mo... [more]
A0A6J1CCA41.8e-11667.24LOW QUALITY PROTEIN: probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Mo... [more]
A0A6J1FP141.2e-10974.23probable long-chain-alcohol O-fatty-acyltransferase 5 isoform X2 OS=Cucurbita mo... [more]
Match NameE-valueIdentityDescription
AT5G55340.13.8e-6342.86MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.15.7e-5941.87MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55360.11.3e-5840.47MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.13.1e-5738.55acyl-CoA sterol acyl transferase 1 [more]
AT5G55350.11.2e-5640.36MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017088Wax synthase, MagnoliopsidaPIRSFPIRSF037006Wax_synthasecoord: 2..349
e-value: 2.3E-113
score: 376.9
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 188..272
e-value: 9.1E-15
score: 54.7
NoneNo IPR availablePANTHERPTHR31595:SF8(MEMBRANE BOUND O-ACYL TRANSFERASE) FAMILY PROTEIN, PUTATIVE-RELATEDcoord: 4..345
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 4..345

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022431.1HG10022431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity