HG10022430 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10022430
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationChr05: 24179537 .. 24180598 (+)
RNA-Seq ExpressionHG10022430
SyntenyHG10022430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTTACCATTCAACATTAGCACCAAACTCCGCCGCCGGAAACGAAATGGACGGCGGCGAACTCCACAGACTGGTCAAGGTATGGACTATCGCCATTGCTTTGATCTCTTACTCATACTATATCGCTTCCAAAATCCCTAAGGGACTCCCAAGGCTCATTGCTCTCTTCCCCGTTATCTTCATCTTCCTCCTCCTTCCTCTCAATCTCCACTCCTTCCATCTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGCAACTTCAAACTCCTCCTCTTCGCTTTCGATCTCGGTCCTCTGTTCTCCTCGCCGCTCCCGAGCCTTCTCCACTTCGCCGCCATGCTCTGCCTTCCGATCAAGATCAACAACCACAAATCCGAGATCCGGAAAATCGTTCTTTCTTTGAAGATCTTGCTTCTAGCGATTGTCATTCGGCTCTACGATTACCGAAATCGGATGGATCCGGCCATCGTTTCCGTTCTGTATTGCCTCCATTTGTACTTCGGCATTGAAATCATCCTCGCTCTGTGTTTTCTTCCGGCAAAAGCCATCTTCGGAGCTACAATCGAACCGCAGTTCGACGAACCCTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCAAGTATCATGCGCCCGACCGTATACGATCCCACCCGTTTCATAACTTCCCGCGTTTTTGGGCCGAGGTGGGCCCAATTCCTCGCCATGGTAGCTACGTTCGCTGTCTCCGGCTTTATGCATGAACTCATCTTTTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCTTACACGGTTTCTGCACGGCTGCGGAGGTGGCCGTTAAGGCCGCCGTGGCTGGAAGGAATGTACGGTGGAAGGTCCACCGGATGGCGTCTGGGTCAATGACTTTGGTGTTTTTGATGATGACAGCACGGTGGCTGATGTTCCCTCCGTTGATTCGAAATGGTGTTGTTGAGAGGACAATTGGCGAGTATTATGCTATGGCCCATTTCGTGAAGGAAAATTTTATTTAG

mRNA sequence

ATGGCGGTTTACCATTCAACATTAGCACCAAACTCCGCCGCCGGAAACGAAATGGACGGCGGCGAACTCCACAGACTGGTCAAGGTATGGACTATCGCCATTGCTTTGATCTCTTACTCATACTATATCGCTTCCAAAATCCCTAAGGGACTCCCAAGGCTCATTGCTCTCTTCCCCGTTATCTTCATCTTCCTCCTCCTTCCTCTCAATCTCCACTCCTTCCATCTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGCAACTTCAAACTCCTCCTCTTCGCTTTCGATCTCGGTCCTCTGTTCTCCTCGCCGCTCCCGAGCCTTCTCCACTTCGCCGCCATGCTCTGCCTTCCGATCAAGATCAACAACCACAAATCCGAGATCCGGAAAATCGTTCTTTCTTTGAAGATCTTGCTTCTAGCGATTGTCATTCGGCTCTACGATTACCGAAATCGGATGGATCCGGCCATCGTTTCCGTTCTGTATTGCCTCCATTTGTACTTCGGCATTGAAATCATCCTCGCTCTGTGTTTTCTTCCGGCAAAAGCCATCTTCGGAGCTACAATCGAACCGCAGTTCGACGAACCCTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCAAGTATCATGCGCCCGACCGTATACGATCCCACCCGTTTCATAACTTCCCGCGTTTTTGGGCCGAGGTGGGCCCAATTCCTCGCCATGGTAGCTACGTTCGCTGTCTCCGGCTTTATGCATGAACTCATCTTTTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCTTACACGGTTTCTGCACGGCTGCGGAGGTGGCCGTTAAGGCCGCCGTGGCTGGAAGGAATGTACGGTGGAAGGTCCACCGGATGGCGTCTGGGTCAATGACTTTGGTGTTTTTGATGATGACAGCACGGTGGCTGATGTTCCCTCCGTTGATTCGAAATGGTGTTGTTGAGAGGACAATTGGCGAGTATTATGCTATGGCCCATTTCGTGAAGGAAAATTTTATTTAG

Coding sequence (CDS)

ATGGCGGTTTACCATTCAACATTAGCACCAAACTCCGCCGCCGGAAACGAAATGGACGGCGGCGAACTCCACAGACTGGTCAAGGTATGGACTATCGCCATTGCTTTGATCTCTTACTCATACTATATCGCTTCCAAAATCCCTAAGGGACTCCCAAGGCTCATTGCTCTCTTCCCCGTTATCTTCATCTTCCTCCTCCTTCCTCTCAATCTCCACTCCTTCCATCTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGCAACTTCAAACTCCTCCTCTTCGCTTTCGATCTCGGTCCTCTGTTCTCCTCGCCGCTCCCGAGCCTTCTCCACTTCGCCGCCATGCTCTGCCTTCCGATCAAGATCAACAACCACAAATCCGAGATCCGGAAAATCGTTCTTTCTTTGAAGATCTTGCTTCTAGCGATTGTCATTCGGCTCTACGATTACCGAAATCGGATGGATCCGGCCATCGTTTCCGTTCTGTATTGCCTCCATTTGTACTTCGGCATTGAAATCATCCTCGCTCTGTGTTTTCTTCCGGCAAAAGCCATCTTCGGAGCTACAATCGAACCGCAGTTCGACGAACCCTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCAAGTATCATGCGCCCGACCGTATACGATCCCACCCGTTTCATAACTTCCCGCGTTTTTGGGCCGAGGTGGGCCCAATTCCTCGCCATGGTAGCTACGTTCGCTGTCTCCGGCTTTATGCATGAACTCATCTTTTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCTTACACGGTTTCTGCACGGCTGCGGAGGTGGCCGTTAAGGCCGCCGTGGCTGGAAGGAATGTACGGTGGAAGGTCCACCGGATGGCGTCTGGGTCAATGACTTTGGTGTTTTTGATGATGACAGCACGGTGGCTGATGTTCCCTCCGTTGATTCGAAATGGTGTTGTTGAGAGGACAATTGGCGAGTATTATGCTATGGCCCATTTCGTGAAGGAAAATTTTATTTAG

Protein sequence

MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKINNHKSEIRKIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI
Homology
BLAST of HG10022430 vs. NCBI nr
Match: XP_038898664.1 (probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 319/361 (88.37%), Postives = 327/361 (90.58%), Query Frame = 0

Query: 1   MAVYHSTLAPNS----AAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIA 60
           MAV+HSTLAPNS    AAG +MDGGE HRL KVWTIAIALISYSYYIASKI KGLPRLIA
Sbjct: 1   MAVHHSTLAPNSAAPTAAGIQMDGGEFHRLAKVWTIAIALISYSYYIASKIHKGLPRLIA 60

Query: 61  LFPVIFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAM 120
           LFPVIFIFLLLPLNLHSFHLCGPTAF LAWLGNFKLLLFAFDLGPLFSSPLPSLLHFA M
Sbjct: 61  LFPVIFIFLLLPLNLHSFHLCGPTAFLLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFATM 120

Query: 121 LCLPIKINNHKSEIRK----IVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGI 180
           LCLPIKINN KSEIR+    IVLS K+LLLAIVIRLYDYRNRMDPAI SVLYCLHLYFGI
Sbjct: 121 LCLPIKINNQKSEIRRTGNQIVLSFKVLLLAIVIRLYDYRNRMDPAIASVLYCLHLYFGI 180

Query: 181 EIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFIT 240
           EI+LALC LPAKAIFGA IEPQFDEPYLSTSLQDFW  RWNLMVPSI+RP VY+PTRFIT
Sbjct: 181 EIVLALCSLPAKAIFGAPIEPQFDEPYLSTSLQDFWGHRWNLMVPSILRPAVYNPTRFIT 240

Query: 241 SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKA 300
           SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAP WEVTWFFVLHGFCTAAEVA KA
Sbjct: 241 SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPMWEVTWFFVLHGFCTAAEVAAKA 300

Query: 301 AVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 354
           AVA R VRWKVHRM SG MTLVFLM TARWLMFP LIRNGVV+RTIGEYY MAHFVK   
Sbjct: 301 AVASRKVRWKVHRMVSGPMTLVFLMATARWLMFPQLIRNGVVDRTIGEYYTMAHFVKGKL 360

BLAST of HG10022430 vs. NCBI nr
Match: XP_008448089.1 (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 3.0e-158
Identity = 288/357 (80.67%), Postives = 311/357 (87.11%), Query Frame = 0

Query: 1   MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPV 60
           M VYHSTL PNS AGN+M+ GE +RL +VWTIAI LI+YSYY ASKIPKG+PRL+ALFPV
Sbjct: 1   MTVYHSTLPPNS-AGNQMNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLALFPV 60

Query: 61  IFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLP 120
           +FIFLLLPLNL SFHLCGPTAFFL+WLGNFKLLLFAFDLGPLFSSPLPS LHFAA LCLP
Sbjct: 61  LFIFLLLPLNLRSFHLCGPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAATLCLP 120

Query: 121 IKINNHKSEIRK----IVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIIL 180
           IKI++HKSEIR+    IVL LK+LLLAIV+RLYDYRNRMDPAIVSVLY LHLYFGIEI+L
Sbjct: 121 IKISSHKSEIRRNGNPIVLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVL 180

Query: 181 ALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVF 240
           A+C LP KAIFGA  EPQFDEPYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR ITSR F
Sbjct: 181 AVCSLPGKAIFGAPFEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAF 240

Query: 241 GPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAG 300
           G RWAQFL MVATF VSG MHELIFYYFTRA PTWEVT FFVLHGFC AAEVA KAAVAG
Sbjct: 241 GRRWAQFLGMVATFVVSGLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEVAAKAAVAG 300

Query: 301 RNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
               W +H M SG ++LVFLM TARWLM P LIRNGVV++ IGEYYAMAHF+K  FI
Sbjct: 301 ---LWNIHPMVSGPISLVFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFLKGKFI 353

BLAST of HG10022430 vs. NCBI nr
Match: KAA0048143.1 (putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa] >TYK08112.1 putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 545.4 bits (1404), Expect = 3.5e-151
Identity = 275/340 (80.88%), Postives = 297/340 (87.35%), Query Frame = 0

Query: 18  MDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLC 77
           M+ GE +RL +VWTIAI LI+YSYY ASKIPKG+PRL+ALFPV+FIFLLLPLNL SFHLC
Sbjct: 1   MNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLALFPVLFIFLLLPLNLRSFHLC 60

Query: 78  GPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKINNHKSEIRK----I 137
           GPTAFFL+WLGNFKLLLFAFDLGPLFSSPLPS LHFAA LCLPIKI++HKSEIR+    I
Sbjct: 61  GPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAATLCLPIKISSHKSEIRRNGNPI 120

Query: 138 VLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEP 197
           VL LK+LLLAIV+RLYDYRNRMDPAIVSVLY LHLYFGIEI+LA+C LP KAIFGA  EP
Sbjct: 121 VLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPFEP 180

Query: 198 QFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVS 257
           QFDEPYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR ITSR FG RWAQFL MVATF VS
Sbjct: 181 QFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFVVS 240

Query: 258 GFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTL 317
           G MHELIFYYFTRA PTWEVT FFVLHGFC AAEVA KAAVAG    W +H M SG ++L
Sbjct: 241 GLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEVAAKAAVAG---LWNIHPMVSGPISL 300

Query: 318 VFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
           VFLM TARWLM P LIRNGVV++ IGEYYAMAHF+K  FI
Sbjct: 301 VFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFLKGKFI 337

BLAST of HG10022430 vs. NCBI nr
Match: KAG7037354.1 (putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 539.7 bits (1389), Expect = 1.9e-149
Identity = 267/361 (73.96%), Postives = 308/361 (85.32%), Query Frame = 0

Query: 1   MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPV 60
           MA  HSTL P SAAGN MD GEL  L++VW+IAIAL+ YSYY+ASKIPKGLPRL++LFPV
Sbjct: 1   MADGHSTLPPTSAAGNLMD-GELSSLLQVWSIAIALMCYSYYVASKIPKGLPRLLSLFPV 60

Query: 61  IFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLP 120
           +F+FLLLPLNLHSFH  GPTAFFLAWLGNFKLLLFAFDLGPL S PLPSLLHFAA+LCLP
Sbjct: 61  LFVFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTSLPLPSLLHFAAVLCLP 120

Query: 121 IKINNHKSE--------IRKIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGI 180
           IKI   K          +  IVLS+K+LLLAI+IRLY YRNR++PA++ VLYCLH+Y GI
Sbjct: 121 IKIKKQKHPEESKNRRIVEPIVLSVKVLLLAIIIRLYSYRNRLNPAMIFVLYCLHMYIGI 180

Query: 181 EIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFIT 240
           EI+LALC LPA+A+ GA IEPQF++PYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR +T
Sbjct: 181 EIVLALCSLPARAMIGAPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSVT 240

Query: 241 SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKA 300
           SR+F P+WAQFLAMV TF VSGFMHEL++YYFTR APTWEVTWFFVLHG CTAAE+A KA
Sbjct: 241 SRIFRPKWAQFLAMVVTFTVSGFMHELMYYYFTRVAPTWEVTWFFVLHGVCTAAELAAKA 300

Query: 301 AVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 354
           AVAGR +RW+V R+ SG MT+ FL+ TARWLMFP LIRNGVVE TIGEYY + +F+K+ F
Sbjct: 301 AVAGRKMRWRVPRVVSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMKDKF 360

BLAST of HG10022430 vs. NCBI nr
Match: XP_023525242.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.3 bits (1383), Expect = 9.6e-149
Identity = 270/361 (74.79%), Postives = 307/361 (85.04%), Query Frame = 0

Query: 1   MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPV 60
           MA  HSTL P SAAGN MD GEL  L++VW+IAIALI YSYY+ASKIPKGLPRL++LFPV
Sbjct: 1   MADGHSTLPPTSAAGNLMD-GELSSLLQVWSIAIALIFYSYYVASKIPKGLPRLLSLFPV 60

Query: 61  IFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLP 120
           +FIFLLLPLNLHSFH  GPTAFFLAWLGNFKLLLFAFDLGPL S PLPSLLHFAA+LCLP
Sbjct: 61  LFIFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTSLPLPSLLHFAAVLCLP 120

Query: 121 IKINNHKSE--------IRKIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGI 180
           IKI   K          +  IVLS+K+LLLAI+IRLY YRNRM+PA++ VLYCLH+Y GI
Sbjct: 121 IKIKKQKHPEESKNRRIVEPIVLSVKVLLLAIIIRLYSYRNRMNPAMIFVLYCLHMYIGI 180

Query: 181 EIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFIT 240
           EI+LALC LPA+A+ GA IEPQF++PYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR IT
Sbjct: 181 EIVLALCSLPARAMIGAPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSIT 240

Query: 241 SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKA 300
           SR+F P+WAQFLAMV TFAVSGFMHEL++YYFTR APTWEVTWFFVLHG  TAAE+A KA
Sbjct: 241 SRIFRPKWAQFLAMVVTFAVSGFMHELMYYYFTRVAPTWEVTWFFVLHGVFTAAELAAKA 300

Query: 301 AVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 354
           AVA R +RW+V R+ SG MT+ FL+ TARWLMFP LIRNGVVE TIGEYY + +F+K+ F
Sbjct: 301 AVAARKMRWRVPRVVSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMKDKF 360

BLAST of HG10022430 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.9e-80
Identity = 162/335 (48.36%), Postives = 216/335 (64.48%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           EL  L+KVW  AI  ISY YY +++I  G+ RL+++ PV  +FL+LPL + S H  G TA
Sbjct: 4   ELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTA 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPS-LLHFAAMLCLPIKINNH---KSEIRKIVLSL 141
           FFL+WL NFKL+LF+FD GPLF  P+PS L  F    C PIK+  +   ++++ K   ++
Sbjct: 64  FFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQNPKPQNQMPKWGFAV 123

Query: 142 KILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDE 201
           K+    +++ +Y+Y+  M P ++ VLY LH+Y   EI+LA   +         +EP F+E
Sbjct: 124 KLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNE 183

Query: 202 PYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMH 261
           PYLSTSLQDFW RRWNLMVP+I+RP VY P R +  R      A FL + A+F VSG +H
Sbjct: 184 PYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVH 243

Query: 262 ELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLM 321
           ELIF+YFTR +PT EVT FFVLHG CTAAE A K     R  RWKV +M S  +T+ F++
Sbjct: 244 ELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVR--RWKVSQMVSRLLTVGFVV 303

Query: 322 MTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 353
           MT  WL FP L R+G++ER   E +    FVK  F
Sbjct: 304 MTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKF 334

BLAST of HG10022430 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.6e-77
Identity = 164/346 (47.40%), Postives = 219/346 (63.29%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPK-GLPRLIALFPVIFIFLLLPLNLHSFHLCGPT 81
           EL    +VW  AIA   Y  ++ +  P  G  RL+ L PV+ +F+ LPL L SFHL GPT
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 82  AFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI-----NNHKS------- 141
           A +L WL NFKLLLFAF LGPL S+P  SLLHF +   LPIK      N+H+        
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLSF 125

Query: 142 EIRKIVLSL-KILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIF 201
           E RK+VL + K++  A ++++Y++R  +   ++SVLYC H Y G EI LA   + A+A  
Sbjct: 126 EWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARATL 185

Query: 202 GATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMV 261
           G  + PQF+EPYL+TSLQDFW RRWNLMV  I+  T Y P R + SR    RW    AM+
Sbjct: 186 GLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAML 245

Query: 262 ATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMA 321
             F VSG MHE+ F+Y TRA P+WEVT FFVLHG CTA E+ VK AV+G   + ++ R  
Sbjct: 246 VAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSG---KVRLRREV 305

Query: 322 SGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
           SG++T+ F+M+T  WL  P L+R+GV  +TI EY  M ++ ++  +
Sbjct: 306 SGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLM 347

BLAST of HG10022430 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.5e-77
Identity = 150/335 (44.78%), Postives = 214/335 (63.88%), Query Frame = 0

Query: 27  VKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTAFFLAW 86
           +K W + I  + Y+++IA  +PKG+ RLI  FPV  IF ++P  ++S HL G TAFF+AW
Sbjct: 5   IKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAW 64

Query: 87  LGNFKLLLFAFDLGPLFSSPLP-SLLHFAAMLCLPIKI---------NNHKSEIR-KIVL 146
           L NFKLLLFA   GPL S+  P SL  F A+ CLPIKI         ++H+      ++ 
Sbjct: 65  LANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLIY 124

Query: 147 SLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQF 206
           ++K + + ++I+ Y+Y  ++   +V  LY +H+YF +EIILA      +A+    +EPQF
Sbjct: 125 TIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQF 184

Query: 207 DEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGF 266
           ++PYL+TSLQDFW RRWNLMV  I+RPTVY+P+  + S V GP ++Q LA   TF VSG 
Sbjct: 185 NKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGI 244

Query: 267 MHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVF 326
           MHELIF+Y  R  P W++ WFF+++GFCT  E+A+K  + G   RW+  +  S  +TL F
Sbjct: 245 MHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTING---RWRFPKAISQVLTLTF 304

Query: 327 LMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKE 351
           +M+TA WL  P   R  +VE+ + EY A+  F  E
Sbjct: 305 VMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVE 335

BLAST of HG10022430 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.3e-76
Identity = 152/331 (45.92%), Postives = 206/331 (62.24%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           +   L++VW  A+  +SY YYI+SK+ KG+ RL+++ PV  +FL+LPL L   H C  + 
Sbjct: 4   KFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISV 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI----------NNHKSEIR 141
            FL+WL NFKLLLFAFD GPLF  P P L  F    CLPIKI          N H   + 
Sbjct: 64  LFLSWLANFKLLLFAFDEGPLFPLP-PKLSRFICFACLPIKIRQDPSPNAIPNLHPKPMP 123

Query: 142 KIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATI 201
           K VL++KIL+L +++ +Y+YR+ +   +V  LYCLH+Y  +E++L         + G  I
Sbjct: 124 KWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNI 183

Query: 202 EPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFA 261
           EP F+EPYL+TSLQDFWSRRWNLMV +++R TV+ P +    R+  P  A F  ++A+F 
Sbjct: 184 EPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFF 243

Query: 262 VSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRW--KVHRMASG 321
           VSG MHEL+++Y  R  PTWEVT FFVLHG  TA E+AVK        +W    HR  SG
Sbjct: 244 VSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVK------RTQWLRPPHRAVSG 303

Query: 322 SMTLVFLMMTARWLMFPPLIRNGVVERTIGE 341
            + L F+ +T  WL    ++RN V E+ IGE
Sbjct: 304 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGE 327

BLAST of HG10022430 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.3e-75
Identity = 155/331 (46.83%), Postives = 205/331 (61.93%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           EL  L+KVW  AI  ISY YYI  +I  G PR +++ PV+ +FL+LPL   S HL   TA
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI-NNHKSE--IRKIVLSLK 141
           FFL WL NFKL+LF+FD GPL   P  +   F    C PIK+  N KS+  + K+V ++K
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIPIP-TNFPRFFCFTCFPIKVQQNPKSQNHLPKLVFAIK 123

Query: 142 ILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDEP 201
           + + A+++ LY YR  + P I+  LY +HLY  IEIIL    +      G  +EPQ ++P
Sbjct: 124 LAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKP 183

Query: 202 YLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMHE 261
           YL+TSLQDFW RRWNLMVP+I+RP VY P R ++ R     WA F  ++A F VSG +HE
Sbjct: 184 YLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHE 243

Query: 262 LIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLMM 321
           L+F+Y  R  PT EVT FFVLHG CTA E+AVK        RW++    S  +T+ F+ +
Sbjct: 244 LLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ-RWRLSPGVSRVLTVGFVFV 303

Query: 322 TARWLMFPPLIRNGVVERTIGEYYAMAHFVK 350
           T  WL  P L R+GV+ER   E   +  F+K
Sbjct: 304 TGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of HG10022430 vs. ExPASy TrEMBL
Match: A0A1S3BIX3 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490376 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 288/357 (80.67%), Postives = 311/357 (87.11%), Query Frame = 0

Query: 1   MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPV 60
           M VYHSTL PNS AGN+M+ GE +RL +VWTIAI LI+YSYY ASKIPKG+PRL+ALFPV
Sbjct: 1   MTVYHSTLPPNS-AGNQMNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLALFPV 60

Query: 61  IFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLP 120
           +FIFLLLPLNL SFHLCGPTAFFL+WLGNFKLLLFAFDLGPLFSSPLPS LHFAA LCLP
Sbjct: 61  LFIFLLLPLNLRSFHLCGPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAATLCLP 120

Query: 121 IKINNHKSEIRK----IVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIIL 180
           IKI++HKSEIR+    IVL LK+LLLAIV+RLYDYRNRMDPAIVSVLY LHLYFGIEI+L
Sbjct: 121 IKISSHKSEIRRNGNPIVLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVL 180

Query: 181 ALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVF 240
           A+C LP KAIFGA  EPQFDEPYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR ITSR F
Sbjct: 181 AVCSLPGKAIFGAPFEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAF 240

Query: 241 GPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAG 300
           G RWAQFL MVATF VSG MHELIFYYFTRA PTWEVT FFVLHGFC AAEVA KAAVAG
Sbjct: 241 GRRWAQFLGMVATFVVSGLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEVAAKAAVAG 300

Query: 301 RNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
               W +H M SG ++LVFLM TARWLM P LIRNGVV++ IGEYYAMAHF+K  FI
Sbjct: 301 ---LWNIHPMVSGPISLVFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFLKGKFI 353

BLAST of HG10022430 vs. ExPASy TrEMBL
Match: A0A5A7TX12 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002780 PE=3 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.7e-151
Identity = 275/340 (80.88%), Postives = 297/340 (87.35%), Query Frame = 0

Query: 18  MDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLC 77
           M+ GE +RL +VWTIAI LI+YSYY ASKIPKG+PRL+ALFPV+FIFLLLPLNL SFHLC
Sbjct: 1   MNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLALFPVLFIFLLLPLNLRSFHLC 60

Query: 78  GPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKINNHKSEIRK----I 137
           GPTAFFL+WLGNFKLLLFAFDLGPLFSSPLPS LHFAA LCLPIKI++HKSEIR+    I
Sbjct: 61  GPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAATLCLPIKISSHKSEIRRNGNPI 120

Query: 138 VLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEP 197
           VL LK+LLLAIV+RLYDYRNRMDPAIVSVLY LHLYFGIEI+LA+C LP KAIFGA  EP
Sbjct: 121 VLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPFEP 180

Query: 198 QFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVS 257
           QFDEPYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR ITSR FG RWAQFL MVATF VS
Sbjct: 181 QFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFVVS 240

Query: 258 GFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTL 317
           G MHELIFYYFTRA PTWEVT FFVLHGFC AAEVA KAAVAG    W +H M SG ++L
Sbjct: 241 GLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEVAAKAAVAG---LWNIHPMVSGPISL 300

Query: 318 VFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
           VFLM TARWLM P LIRNGVV++ IGEYYAMAHF+K  FI
Sbjct: 301 VFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFLKGKFI 337

BLAST of HG10022430 vs. ExPASy TrEMBL
Match: A0A6J1IU70 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480612 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.8e-148
Identity = 267/361 (73.96%), Postives = 304/361 (84.21%), Query Frame = 0

Query: 1   MAVYHSTLAPNSAAGNEMDGGELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPV 60
           MA  HSTL P SAAGN MD GEL  L++VW+IAIALI YSYY+ASKI KGLPRL++LFPV
Sbjct: 1   MADGHSTLPPTSAAGNLMD-GELSSLLQVWSIAIALICYSYYVASKIAKGLPRLLSLFPV 60

Query: 61  IFIFLLLPLNLHSFHLCGPTAFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLP 120
           +FIFLLLPLNLHSFH  GPTAFFLAWLGNFKLLLFAFDLGPL   PLPSLLHFAA+LCLP
Sbjct: 61  LFIFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTYLPLPSLLHFAAVLCLP 120

Query: 121 IKINNHKSE--------IRKIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGI 180
           IKI   K          +  IVLS+KILLLAI+IRLY YRNRM+PA++ VLYCLH+Y GI
Sbjct: 121 IKIKKQKHPEESKNRRIVEPIVLSVKILLLAIIIRLYSYRNRMNPAMIFVLYCLHMYIGI 180

Query: 181 EIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFIT 240
           EI+LALC LP++A+ G  IEPQF++PYLSTSLQDFWSRRWNLMVPSI+RP VYDPTR +T
Sbjct: 181 EIVLALCSLPSRAMIGEPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSLT 240

Query: 241 SRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKA 300
           SR+F P+WAQFLAMV TFAVSG MHEL++YYFTR APTWEVTWFFVLHG CTAAEVA KA
Sbjct: 241 SRIFRPKWAQFLAMVVTFAVSGLMHELMYYYFTRVAPTWEVTWFFVLHGVCTAAEVAAKA 300

Query: 301 AVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 354
           AV GR +RW+V R+ SG MT+ FL+ TARWLMFP LIRNGVVE TIGEYY + +F+K+ F
Sbjct: 301 AVTGRKIRWRVPRVLSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMKDKF 360

BLAST of HG10022430 vs. ExPASy TrEMBL
Match: A0A6J5X6S6 (MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS28007 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 5.1e-103
Identity = 196/371 (52.83%), Postives = 250/371 (67.39%), Query Frame = 0

Query: 21  GELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPT 80
           GE++  +KVW +    + Y YYIA+KIPKG+ RLI+L P+ +IF++LPLNLHSFHLCGPT
Sbjct: 3   GEINNFIKVWILTNTSLCYCYYIAAKIPKGIIRLISLLPIFYIFIILPLNLHSFHLCGPT 62

Query: 81  AFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI----------------- 140
            FFL WLG FKLLLF+F+LGPL  +P P+L+ F ++ CLPIKI                 
Sbjct: 63  TFFLVWLGIFKLLLFSFNLGPLSPTP-PTLVQFISIACLPIKIKQNPPQKSPQNTDQNPL 122

Query: 141 --NNHKSEI-----------------------RKIVLSLKILLLAIVIRLYDYRNRMDPA 200
              N+K+                         + I+L++K LLLA++IR Y+YR  + P 
Sbjct: 123 TPQNNKNPSHQTTPKPKKSVNPIDQTLTNVPNKSILLAIKALLLALIIRTYEYRPHLHPY 182

Query: 201 IVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPS 260
           I+  LYC H+Y GIE++LAL  +PA+AI G  +EPQF EPYLSTSLQDFW RRWNLMV +
Sbjct: 183 IILALYCCHMYLGIELVLALSAVPARAILGFELEPQFTEPYLSTSLQDFWGRRWNLMVTN 242

Query: 261 IMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFV 320
           I+RP  YDP R I+ R+ GPRWA+FLA+++TFAVSG MHE I+YY TR +PTWEVTWFFV
Sbjct: 243 ILRPVAYDPVRRISMRILGPRWARFLAVMSTFAVSGLMHEAIYYYLTRVSPTWEVTWFFV 302

Query: 321 LHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTI 350
           LHG C A EV VK A A    RW++H + SG +T+VFL +T  WL FP L+RNGV  + I
Sbjct: 303 LHGVCVAVEVEVKKAAAD---RWRLHPVVSGPLTIVFLAVTGNWLFFPQLLRNGVDLKAI 362

BLAST of HG10022430 vs. ExPASy TrEMBL
Match: A0A6J5UNH8 (MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28346 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 5.1e-103
Identity = 196/371 (52.83%), Postives = 250/371 (67.39%), Query Frame = 0

Query: 21  GELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPT 80
           GE++  +KVW +    + Y YYIA+KIPKG+ RLI+L P+ +IF++LPLNLHSFHLCGPT
Sbjct: 3   GEINNFIKVWILTNTSLCYCYYIAAKIPKGIIRLISLLPIFYIFIILPLNLHSFHLCGPT 62

Query: 81  AFFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI----------------- 140
            FFL WLG FKLLLF+F+LGPL  +P P+L+ F ++ CLPIKI                 
Sbjct: 63  TFFLVWLGIFKLLLFSFNLGPLSPTP-PTLVQFISIACLPIKIKQNPPQKSPQNTDQNPL 122

Query: 141 --NNHKSEI-----------------------RKIVLSLKILLLAIVIRLYDYRNRMDPA 200
              N+K+                         + I+L++K LLLA++IR Y+YR  + P 
Sbjct: 123 TPQNNKNPSHQTTPKPKKSVNPIDQTLTNVPNKSILLAIKALLLALIIRTYEYRPHLHPY 182

Query: 201 IVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDEPYLSTSLQDFWSRRWNLMVPS 260
           I+  LYC H+Y GIE++LAL  +PA+AI G  +EPQF EPYLSTSLQDFW RRWNLMV +
Sbjct: 183 IILALYCCHMYLGIELVLALSAVPARAILGFELEPQFTEPYLSTSLQDFWGRRWNLMVTN 242

Query: 261 IMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPTWEVTWFFV 320
           I+RP  YDP R I+ R+ GPRWA+FLA+++TFAVSG MHE I+YY TR +PTWEVTWFFV
Sbjct: 243 ILRPVAYDPVRRISMRILGPRWARFLAVMSTFAVSGLMHEAIYYYLTRVSPTWEVTWFFV 302

Query: 321 LHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLMMTARWLMFPPLIRNGVVERTI 350
           LHG C A EV VK A A    RW++H + SG +T+VFL +T  WL FP L+RNGV  + I
Sbjct: 303 LHGVCVAVEVEVKKAAAD---RWRLHPVVSGPLTIVFLAVTGNWLFFPQLLRNGVDLKAI 362

BLAST of HG10022430 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 298.5 bits (763), Expect = 7.0e-81
Identity = 162/335 (48.36%), Postives = 216/335 (64.48%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           EL  L+KVW  AI  ISY YY +++I  G+ RL+++ PV  +FL+LPL + S H  G TA
Sbjct: 4   ELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTA 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPS-LLHFAAMLCLPIKINNH---KSEIRKIVLSL 141
           FFL+WL NFKL+LF+FD GPLF  P+PS L  F    C PIK+  +   ++++ K   ++
Sbjct: 64  FFLSWLANFKLILFSFDQGPLF--PVPSNLSRFVCFTCFPIKLQQNPKPQNQMPKWGFAV 123

Query: 142 KILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDE 201
           K+    +++ +Y+Y+  M P ++ VLY LH+Y   EI+LA   +         +EP F+E
Sbjct: 124 KLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNE 183

Query: 202 PYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMH 261
           PYLSTSLQDFW RRWNLMVP+I+RP VY P R +  R      A FL + A+F VSG +H
Sbjct: 184 PYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVH 243

Query: 262 ELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLM 321
           ELIF+YFTR +PT EVT FFVLHG CTAAE A K     R  RWKV +M S  +T+ F++
Sbjct: 244 ELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVR--RWKVSQMVSRLLTVGFVV 303

Query: 322 MTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENF 353
           MT  WL FP L R+G++ER   E +    FVK  F
Sbjct: 304 MTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKF 334

BLAST of HG10022430 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 290.0 bits (741), Expect = 2.5e-78
Identity = 150/335 (44.78%), Postives = 214/335 (63.88%), Query Frame = 0

Query: 27  VKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTAFFLAW 86
           +K W + I  + Y+++IA  +PKG+ RLI  FPV  IF ++P  ++S HL G TAFF+AW
Sbjct: 5   IKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAW 64

Query: 87  LGNFKLLLFAFDLGPLFSSPLP-SLLHFAAMLCLPIKI---------NNHKSEIR-KIVL 146
           L NFKLLLFA   GPL S+  P SL  F A+ CLPIKI         ++H+      ++ 
Sbjct: 65  LANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLIY 124

Query: 147 SLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQF 206
           ++K + + ++I+ Y+Y  ++   +V  LY +H+YF +EIILA      +A+    +EPQF
Sbjct: 125 TIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQF 184

Query: 207 DEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGF 266
           ++PYL+TSLQDFW RRWNLMV  I+RPTVY+P+  + S V GP ++Q LA   TF VSG 
Sbjct: 185 NKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVSGI 244

Query: 267 MHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVF 326
           MHELIF+Y  R  P W++ WFF+++GFCT  E+A+K  + G   RW+  +  S  +TL F
Sbjct: 245 MHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTING---RWRFPKAISQVLTLTF 304

Query: 327 LMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKE 351
           +M+TA WL  P   R  +VE+ + EY A+  F  E
Sbjct: 305 VMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVE 335

BLAST of HG10022430 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-77
Identity = 152/331 (45.92%), Postives = 206/331 (62.24%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           +   L++VW  A+  +SY YYI+SK+ KG+ RL+++ PV  +FL+LPL L   H C  + 
Sbjct: 4   KFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCAISV 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI----------NNHKSEIR 141
            FL+WL NFKLLLFAFD GPLF  P P L  F    CLPIKI          N H   + 
Sbjct: 64  LFLSWLANFKLLLFAFDEGPLFPLP-PKLSRFICFACLPIKIRQDPSPNAIPNLHPKPMP 123

Query: 142 KIVLSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATI 201
           K VL++KIL+L +++ +Y+YR+ +   +V  LYCLH+Y  +E++L         + G  I
Sbjct: 124 KWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLLGCNI 183

Query: 202 EPQFDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFA 261
           EP F+EPYL+TSLQDFWSRRWNLMV +++R TV+ P +    R+  P  A F  ++A+F 
Sbjct: 184 EPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVMASFF 243

Query: 262 VSGFMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRW--KVHRMASG 321
           VSG MHEL+++Y  R  PTWEVT FFVLHG  TA E+AVK        +W    HR  SG
Sbjct: 244 VSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVK------RTQWLRPPHRAVSG 303

Query: 322 SMTLVFLMMTARWLMFPPLIRNGVVERTIGE 341
            + L F+ +T  WL    ++RN V E+ IGE
Sbjct: 304 LVVLTFVSVTGVWLFLAQVLRNNVHEKAIGE 327

BLAST of HG10022430 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.1e-76
Identity = 155/331 (46.83%), Postives = 205/331 (61.93%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           EL  L+KVW  AI  ISY YYI  +I  G PR +++ PV+ +FL+LPL   S HL   TA
Sbjct: 4   ELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITA 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFAAMLCLPIKI-NNHKSE--IRKIVLSLK 141
           FFL WL NFKL+LF+FD GPL   P  +   F    C PIK+  N KS+  + K+V ++K
Sbjct: 64  FFLTWLANFKLILFSFDKGPLIPIP-TNFPRFFCFTCFPIKVQQNPKSQNHLPKLVFAIK 123

Query: 142 ILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQFDEP 201
           + + A+++ LY YR  + P I+  LY +HLY  IEIIL    +      G  +EPQ ++P
Sbjct: 124 LAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKP 183

Query: 202 YLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSGFMHE 261
           YL+TSLQDFW RRWNLMVP+I+RP VY P R ++ R     WA F  ++A F VSG +HE
Sbjct: 184 YLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHE 243

Query: 262 LIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLVFLMM 321
           L+F+Y  R  PT EVT FFVLHG CTA E+AVK        RW++    S  +T+ F+ +
Sbjct: 244 LLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ-RWRLSPGVSRVLTVGFVFV 303

Query: 322 TARWLMFPPLIRNGVVERTIGEYYAMAHFVK 350
           T  WL  P L R+GV+ER   E   +  F+K
Sbjct: 304 TGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of HG10022430 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 150/339 (44.25%), Postives = 211/339 (62.24%), Query Frame = 0

Query: 22  ELHRLVKVWTIAIALISYSYYIASKIPKGLPRLIALFPVIFIFLLLPLNLHSFHLCGPTA 81
           EL   +K+W  AI  ISY YY+++ I  G+ RL+++ PV  +FL+ PL     H  G  A
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 82  FFLAWLGNFKLLLFAFDLGPLFSSPLP-SLLHFAAMLCLPIK------INNHKSEIRKIV 141
           FFL+WL NFKL+LF+FD GPL  SPLP +L  F  + C PIK      I N+K  I   +
Sbjct: 64  FFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYKIPI--WL 123

Query: 142 LSLKILLLAIVIRLYDYRNRMDPAIVSVLYCLHLYFGIEIILALCFLPAKAIFGATIEPQ 201
            ++K+++  +++++Y+Y+  + PA++ V   LH++  +EI+  L         G  +EPQ
Sbjct: 124 FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQ 183

Query: 202 FDEPYLSTSLQDFWSRRWNLMVPSIMRPTVYDPTRFITSRVFGPRWAQFLAMVATFAVSG 261
            +EPYL+TSLQDFW RRWNLMVP+I+RP VY P R +  R      A FL + A F VSG
Sbjct: 184 SNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSG 243

Query: 262 FMHELIFYYFTRAAPTWEVTWFFVLHGFCTAAEVAVKAAVAGRNVRWKVHRMASGSMTLV 321
            +HE++F+Y TR  PT EVTWFF+LHG CT AEVAVK +   R   W+V    S  +T+ 
Sbjct: 244 AVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRR-WWRVSPTVSRLLTVG 303

Query: 322 FLMMTARWLMFPPLIRNGVVERTIGEYYAMAHFVKENFI 354
           F+++T+ W  F PLIR+G++ER   E      FVK  F+
Sbjct: 304 FVVVTSGWFFF-PLIRSGIIERLASEALMCIDFVKHKFL 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898664.11.6e-17588.37probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida][more]
XP_008448089.13.0e-15880.67PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
KAA0048143.13.5e-15180.88putative long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo var. makuwa]... [more]
KAG7037354.11.9e-14973.96putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyro... [more]
XP_023525242.19.6e-14974.79probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
Q9FJ759.9e-8048.36Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX... [more]
Q9XGY61.6e-7747.40Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ... [more]
Q9SV073.5e-7744.78Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Q9FJ722.3e-7645.92Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9FJ764.3e-7546.83Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A1S3BIX31.4e-15880.67probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7TX121.7e-15180.88Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw... [more]
A0A6J1IU708.8e-14873.96probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366... [more]
A0A6J5X6S65.1e-10352.83MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCU... [more]
A0A6J5UNH85.1e-10352.83MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28... [more]
Match NameE-valueIdentityDescription
AT5G55350.17.0e-8148.36MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.12.5e-7844.78acyl-CoA sterol acyl transferase 1 [more]
AT5G55380.11.6e-7745.92MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55340.13.1e-7646.83MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55360.11.2e-7244.25MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017088Wax synthase, MagnoliopsidaPIRSFPIRSF037006Wax_synthasecoord: 19..353
e-value: 1.3E-125
score: 417.1
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 192..278
e-value: 1.8E-16
score: 60.1
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 21..350
NoneNo IPR availablePANTHERPTHR31595:SF46ACYL-COA--STEROL O-ACYLTRANSFERASE 1coord: 21..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10022430.1HG10022430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity