HG10022397 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022397
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Two-pore potassium channel like
Location	Chr05: 23874461 .. 23875699 (-)
RNA-Seq Expression	HG10022397
Synteny	HG10022397

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCGTCTAATACTTCTGGAAAAAGGTTAATAGATATTCCTAGAAGGAAAAGACGATTGAGGCGCACTAAGAGTGCTCCTCATGCAGTTTCTCCTCACACAGAGATCACCTGCACCAGCAACGTTCCAGCCACTGGTCCAGTTCCACGTTCAGGATTAATTTTTGGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATAATATACCTGGGTATTGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAATGGAATAGTCGATGCCATTTATTTCACTATTGTGACAATGACAACTGTTGGATATGGAGATCTTGTCCCAAACAGTCCTTCCGCAAAACTACTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTGGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATGCACCAAAATGGCCATTGTGACATTACAAAAGAAATTGATACCAATAAAGCTAGAAACAAGTGTATTGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGTCTTTCTAGTTACCCTTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCAACCATCACAACGCTAGGCTATGGAGATAAAAGCTTCTCGACCAGGTGGGGGCGTCTGTTCGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAGAGAAGGCAAAAGTCTCTGGTAAAGTGGGTTCTATGTAAAAAGGTGACAGACTTAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTCGTCGGGTAAGTCATAGTCTATTCTTTTCATCACACTCCCCTATTATGTGGTCTCAAACTAACTCAGTCAGAACACATCAATTAATTTCTGATGAGACCCCGTTTGACGATCCCGAGTTGGAGTTTATTAAATTTCCATAGCTGTTCACTTATCCTATCCTTCTTAATCTTCCACAGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGAAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTATATCTGATATAACACTTGCTCAACTATCTTAA

mRNA sequence

ATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCGTCTAATACTTCTGGAAAAAGGTTAATAGATATTCCTAGAAGGAAAAGACGATTGAGGCGCACTAAGAGTGCTCCTCATGCAGTTTCTCCTCACACAGAGATCACCTGCACCAGCAACGTTCCAGCCACTGGTCCAGTTCCACGTTCAGGATTAATTTTTGGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATAATATACCTGGGTATTGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAATGGAATAGTCGATGCCATTTATTTCACTATTGTGACAATGACAACTGTTGGATATGGAGATCTTGTCCCAAACAGTCCTTCCGCAAAACTACTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTGGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATGCACCAAAATGGCCATTGTGACATTACAAAAGAAATTGATACCAATAAAGCTAGAAACAAGTGTATTGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGTCTTTCTAGTTACCCTTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCAACCATCACAACGCTAGGCTATGGAGATAAAAGCTTCTCGACCAGGTGGGGGCGTCTGTTCGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAGAGAAGGCAAAAGTCTCTGGTAAAGTGGGTTCTATGTAAAAAGGTGACAGACTTAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGAAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTATATCTGATATAACACTTGCTCAACTATCTTAA

Coding sequence (CDS)

ATGGGTAGCCAAGAAGCTAGACAGCCACTGTTACCAACATCGTCTAATACTTCTGGAAAAAGGTTAATAGATATTCCTAGAAGGAAAAGACGATTGAGGCGCACTAAGAGTGCTCCTCATGCAGTTTCTCCTCACACAGAGATCACCTGCACCAGCAACGTTCCAGCCACTGGTCCAGTTCCACGTTCAGGATTAATTTTTGGAAATCTACACCCAAGTTTCAGAAGAGTAGCCTTAGTTTTAATAATATACCTGGGTATTGGAACTTTATGTTTTTACCTTGTCAGGTACCAAATCAAAGGGGAAAAAACAAATGGAATAGTCGATGCCATTTATTTCACTATTGTGACAATGACAACTGTTGGATATGGAGATCTTGTCCCAAACAGTCCTTCCGCAAAACTACTAGCTTGTGCTTTCGTTTTCACAGGAATGGCCCTAATTGGCCTAATACTGAGCAATGCAGCCGATTATTTGGTGGAGAAGCAGGAAATCTTGCTATTTAAAGCCTTCCATATGCACCAAAATGGCCATTGTGACATTACAAAAGAAATTGATACCAATAAAGCTAGAAACAAGTGTATTGTGGTGTTTCTCATTCTTCTTTTGTTCATCATTTCTGGGACTGTCTTTCTAGTTACCCTTGAGAAGTTAGATTTTATTGATGCCTTTTATTGTGTATGTTCAACCATCACAACGCTAGGCTATGGAGATAAAAGCTTCTCGACCAGGTGGGGGCGTCTGTTCGCCATTTTCTGGATCTTGATAAGTACCATCACTCTAGCTCAGTTTTTCCTTTACATTGCTGAGCTAAACACTGAGAGAAGGCAAAAGTCTCTGGTAAAGTGGGTTCTATGTAAAAAGGTGACAGACTTAGACCTCGAGGTTGCAGATCTCGATGATGATGGGGTCGTCGGGGCTGCAGAATTTGTCATATACAAGCTCAAGGAGATGGGGAAGATTACTGAAGATGACATTTCACCTGTGTTGAAGGAATTTGAGAATCTTGATGTTGATCAATCAGGAACTCTGTCTATATCTGATATAACACTTGCTCAACTATCTTAA

Protein sequence

MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCDITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS

Homology

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BLAST of HG10022397 vs. NCBI nr
Match: XP_038896135.1 (two-pore potassium channel 1 [Benincasa hispida])

HSP 1 Score: 641.3 bits (1653), Expect = 4.8e-180
Identity = 330/355 (92.96%), Postives = 339/355 (95.49%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQEARQPLLPTSSNT GKRLIDIPR +RRLRRTKSAP+A SPHTEI CT NVPAT PV
Sbjct: 1   MGSQEARQPLLPTSSNTPGKRLIDIPRSRRRLRRTKSAPYADSPHTEIACTGNVPATCPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSG IF NLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGSIFRNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVFTGMALIGLILSNAADYLVEKQE LLFKAFHMHQN HCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALIGLILSNAADYLVEKQETLLFKAFHMHQNSHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I++EIDTNKARNKCIVVFLILLLFIISGT FLV+LEKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISREIDTNKARNKCIVVFLILLLFIISGTAFLVSLEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST WGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKW+L KKVTD+DLEVADLD
Sbjct: 241 FSTMWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWILSKKVTDIDLEVADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL+EFENLDVDQSGTLS SDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLEEFENLDVDQSGTLSTSDITLAQSS 355

[Close]

BLAST of HG10022397 vs. NCBI nr
Match: TYK07941.1 (two-pore potassium channel 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 638.3 bits (1645), Expect = 4.0e-179
Identity = 328/355 (92.39%), Postives = 343/355 (96.62%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGS++ARQPLLPTSSNT   R+IDIPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSEDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVF+GMAL+GLILSNAADYLVEKQEILLFKAFH+HQNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKC+VVFL+LLLFIISGT FLV +EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL KKVTD+DLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. NCBI nr
Match: XP_008462994.1 (PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo])

HSP 1 Score: 634.8 bits (1636), Expect = 4.5e-178
Identity = 326/355 (91.83%), Postives = 343/355 (96.62%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGS++ARQPLLPTSSNT   R+IDIPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEK+NGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVF+GMAL+GLILSNAADYLVEKQEILLFKAFH+HQNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKC+VVFL+LLLFIISGT FLV +EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL +KVTD+DLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. NCBI nr
Match: XP_004150789.1 (two-pore potassium channel 1 [Cucumis sativus] >KGN44008.1 hypothetical protein Csa_018211 [Cucumis sativus])

HSP 1 Score: 625.2 bits (1611), Expect = 3.5e-175
Identity = 324/355 (91.27%), Postives = 340/355 (95.77%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQ+ARQPLLPTSSNT   R+I+IPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSP-TEITHTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLI YLGIGTLCFYLVR+QI+GEKTN +VDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVFTGMAL+GLILSNAADYLVEKQEILLFKAFH+ QNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKCIVVFL+LLLFIISGT FLVT+EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL KKVTD+DLEVAD+D
Sbjct: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDIS VL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. NCBI nr
Match: KAG7024969.1 (Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 597.8 bits (1540), Expect = 6.0e-167
Identity = 312/355 (87.89%), Postives = 326/355 (91.83%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQE RQP+LPTSSNT   R IDIPR KRRLRRTKSAPH+ SPHTE T T     TG V
Sbjct: 1   MGSQEVRQPMLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSDSPHTETTGT----VTGLV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGL+FGNLHPSF RVALVLI+YLGIGTLCFYLVR+QIKG KTNG+VDAIYFTIVTMTT
Sbjct: 61  PRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYLVRHQIKGGKTNGVVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPN+PS KLLACAFVFTGMA++GLIL+NAADYLVEKQEI LFK FHMHQNG CD
Sbjct: 121 VGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKVFHMHQNGSCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           IT+EIDTNKARNKCIVVFL LLLFII GTVFLVTLE+LDFIDAFYCVCSTITTLGYGDKS
Sbjct: 181 ITEEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FSTRWGRLFAIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVL KKVT LDLE ADLD
Sbjct: 241 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

[Close]

BLAST of HG10022397 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-111
Identity = 215/359 (59.89%), Postives = 268/359 (74.65%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDI-----PRRKRRLRRTKSAPHAVSPHTEITCTSNVP 60
           M S  AR PLLPT    +  +  ++       RKRRLRR++SAP     + +       P
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPP 60

Query: 61  ATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTI 120
              P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+VDA+YF I
Sbjct: 61  ---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCI 120

Query: 121 VTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQ 180
           VTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL +AFH+ Q
Sbjct: 121 VTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQ 180

Query: 181 N-GHCDITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTL 240
           + G  DI KE+ TNK R KC    L+L++  I GT+FLV +EK+  I AFYCVCST+TTL
Sbjct: 181 SFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTL 240

Query: 241 GYGDKSFSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDL 300
           GYGDKSF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKWVL +++T+ DL
Sbjct: 241 GYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDL 300

Query: 301 EVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQ 354
           E ADLD+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD D+SGTL+ SDI LAQ
Sbjct: 301 EAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQ 356

[Close]

BLAST of HG10022397 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.9e-97
Identity = 191/354 (53.95%), Postives = 247/354 (69.77%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           M     +Q LL  + N   ++  +   R RR R T S      P          P  G  
Sbjct: 1   MDDNSIQQSLLADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGP----------PEKGSS 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
            ++  +F  + PSFR V L+L IYL +G L FY V  +I G++TN ++DA+YF +VTMTT
Sbjct: 61  VKAKELFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMH-QNGHC 180
           VGYGDLVPN+ + KLLACAFVF GMA++ L +S  ADYLVEKQE+L FKA H + + G  
Sbjct: 121 VGYGDLVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGET 180

Query: 181 DITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDK 240
            + + I+TN+ + K     L+L+L IISGTVFL  +EKL  +D+FYCVC+TITTLGYGDK
Sbjct: 181 KMLRAIETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDK 240

Query: 241 SFSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADL 300
           SFS++ GR+FA+FWI+ STI +AQFF+Y+AE+ TERRQK L  WVL +K+T +DLE ADL
Sbjct: 241 SFSSKLGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADL 300

Query: 301 DDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQ 354
           DDD  VGAAEFV+YKLKE+GKI +++IS  L+EFE LDVD SGTLS  D+TLAQ
Sbjct: 301 DDDRQVGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDLTLAQ 344

[Close]

BLAST of HG10022397 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.8e-88
Identity = 174/324 (53.70%), Postives = 226/324 (69.75%), Query Frame = 0


Query: 30  RRLRRTKSAPHAVSPHTEITCTSNVPATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGT 89
           RR RR ++AP +  P T+   +S   A    P   L  G   PSFR V L+L+ YL +GT
Sbjct: 25  RRFRRCRTAPSSEPPPTDKDNSSAADA----PPKTLFTGGGRPSFRLVGLLLVAYLLLGT 84

Query: 90  LCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIG 149
           + FYL    + G +T   +DA+YF +VTMTTVGYGDLVP S +AKLLACAFVF G+A++G
Sbjct: 85  IAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDAAKLLACAFVFAGVAVVG 144

Query: 150 LILSNAADYLVEKQEILLFKAFHMHQNGHCDITKEIDTNKARNKCIVVFLILLLFIISGT 209
             LS AADYLVEKQE LLF+A H H      + + ++ NK R K     L+L+  + SGT
Sbjct: 145 TFLSKAADYLVEKQEALLFRALHSH-----TMVRAMEMNKVRYKLYTAGLLLVAAVASGT 204

Query: 210 VFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILISTITLAQFFLYIA 269
           V L  +E +  +DAFYCVC+T+TTLGYGD+SFS+  GR FA+ WI +ST+ +A FFLY A
Sbjct: 205 VVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAVAWITVSTVVVALFFLYAA 264

Query: 270 ELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPV 329
           EL TERRQ+ L +WVL ++ T++DLE ADLD D  VGAA+FV+YKLKE+GKI+++DIS  
Sbjct: 265 ELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFVLYKLKELGKISQEDISEF 324

Query: 330 LKEFENLDVDQSGTLSISDITLAQ 354
           L EF+NLD D SGTLS +D+  AQ
Sbjct: 325 LDEFDNLDADHSGTLSPADLAAAQ 339

[Close]

BLAST of HG10022397 vs. ExPASy Swiss-Prot
Match: Q9S6Z8 (Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.0e-47
Identity = 117/331 (35.35%), Postives = 184/331 (55.59%), Query Frame = 0


Query: 30  RRLRRTKSAPHAVSPHTEITCTSNVPATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGT 89
           R L R+++AP  V      T         PV +S           R+   +LI+YL +G 
Sbjct: 82  RPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKS---------IIRQAIFLLIVYLTLGV 141

Query: 90  LCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIG 149
             +   R    G +T+ +VDA+YF IVTM T+GYGD+ P +P  K+ A  FV  G   + 
Sbjct: 142 SIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLD 201

Query: 150 LILSNAADYLVEKQEILLF-----KAFHMHQNGHCDITKE--IDTNKA----RNKCIVVF 209
           ++LS   +Y+++ QE ++      +  H H + H    K+  ID  K     R K  +  
Sbjct: 202 ILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLAL 261

Query: 210 LILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILIST 269
            +++L I  G + L  +E+L F+D+ Y    ++TT+GYGD++F T  GRLFA  W+L+ST
Sbjct: 262 CVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVST 321

Query: 270 ITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEFVIYKLKEM 329
           + +A+ FLY+AE   +RR +  VK  L +++T  DL  AD    G +  +E+++ KLKEM
Sbjct: 322 LAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFISKSEYIVLKLKEM 381

Query: 330 GKITEDDISPVLKEFENLDVDQSGTLSISDI 350
           GKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 382 GKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of HG10022397 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 103/279 (36.92%), Postives = 166/279 (59.50%), Query Frame = 0


Query: 75  RRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAK 134
           R+   +L++YL +G L ++L R      +T+ +VD +YF IVTM T+GYGD+ PNS   K
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 135 LLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCDITKEIDTNKARN-- 194
           L +  FV  G   I ++LS    Y+++ QE  +  +           +  ID  K R   
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRI 270

Query: 195 --KCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFA 254
             K  +   +++L I  G   +  +E++ ++D+FY    ++TT+GYGD++F T  GRLFA
Sbjct: 271 RLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFA 330

Query: 255 IFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEF 314
             W+L+ST+ +A+ FLY+AE   ++R +   K VLC+ ++      AD+D++G V  AE+
Sbjct: 331 AIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEY 390

Query: 315 VIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDI 350
           VIYKLKEM KIT+ DI P+ K+F+ LD   +G +++ D+
Sbjct: 391 VIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

[Close]

BLAST of HG10022397 vs. ExPASy TrEMBL
Match: A0A5D3C7V3 (Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00580 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.0e-179
Identity = 328/355 (92.39%), Postives = 343/355 (96.62%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGS++ARQPLLPTSSNT   R+IDIPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSEDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVF+GMAL+GLILSNAADYLVEKQEILLFKAFH+HQNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKC+VVFL+LLLFIISGT FLV +EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL KKVTD+DLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. ExPASy TrEMBL
Match: A0A1S3CI53 (two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.2e-178
Identity = 326/355 (91.83%), Postives = 343/355 (96.62%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGS++ARQPLLPTSSNT   R+IDIPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSKDARQPLLPTSSNTLETRVIDIPRSKRRLRRTKSAPHANSP-TEITRTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEK+NGIVDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKSNGIVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVF+GMAL+GLILSNAADYLVEKQEILLFKAFH+HQNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFSGMALVGLILSNAADYLVEKQEILLFKAFHIHQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKC+VVFL+LLLFIISGT FLV +EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCVVVFLLLLLFIISGTAFLVIIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL +KVTD+DLEVAD+D
Sbjct: 241 FSTKWGRIFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSEKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. ExPASy TrEMBL
Match: A0A0A0K3G0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 324/355 (91.27%), Postives = 340/355 (95.77%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQ+ARQPLLPTSSNT   R+I+IPR KRRLRRTKSAPHA SP TEIT TSNVPATGPV
Sbjct: 1   MGSQDARQPLLPTSSNTLETRVINIPRSKRRLRRTKSAPHANSP-TEITHTSNVPATGPV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGLIFGNLHPSFRRVALVLI YLGIGTLCFYLVR+QI+GEKTN +VDAIYFTIVTMTT
Sbjct: 61  PRSGLIFGNLHPSFRRVALVLITYLGIGTLCFYLVRHQIQGEKTNRLVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPNSPS KLLACAFVFTGMAL+GLILSNAADYLVEKQEILLFKAFH+ QNGHCD
Sbjct: 121 VGYGDLVPNSPSTKLLACAFVFTGMALVGLILSNAADYLVEKQEILLFKAFHIDQNGHCD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           I+KEIDTNKARNKCIVVFL+LLLFIISGT FLVT+EKLDFIDAFYCVCSTITTLGYGD+S
Sbjct: 181 ISKEIDTNKARNKCIVVFLLLLLFIISGTAFLVTIEKLDFIDAFYCVCSTITTLGYGDQS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FST+WGR+FAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVL KKVTD+DLEVAD+D
Sbjct: 241 FSTKWGRVFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLSKKVTDIDLEVADID 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDIS VL EFENLDVDQSGTLSISDITLAQLS
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISLVLNEFENLDVDQSGTLSISDITLAQLS 354

[Close]

BLAST of HG10022397 vs. ExPASy TrEMBL
Match: A0A6J1FCF3 (two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 313/355 (88.17%), Postives = 326/355 (91.83%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQE RQP+LPTSSNT   R IDIPR KRRLRRTKSAPH+ SPHTE T T    ATG V
Sbjct: 1   MGSQEVRQPMLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTST----ATGLV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGL+FGNLHPSF RVALVLI+YLGIGTLCFYLV +QIKG KTNG+VDAIYFTIVTMTT
Sbjct: 61  PRSGLVFGNLHPSFIRVALVLIMYLGIGTLCFYLVGHQIKGGKTNGVVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPN+PS KLLACAFVFTGMA++GLIL+NAADYLVEKQEI LFKAFHMHQNG  D
Sbjct: 121 VGYGDLVPNTPSTKLLACAFVFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGPFD 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
           ITKEIDTNKARNKCIVVFL LLLFII GTVFLVTLE+LDFIDAFYCVCSTITTLGYGDKS
Sbjct: 181 ITKEIDTNKARNKCIVVFLFLLLFIIFGTVFLVTLEELDFIDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FSTRWGRLFAIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVL KKVT LDLE ADLD
Sbjct: 241 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

[Close]

BLAST of HG10022397 vs. ExPASy TrEMBL
Match: A0A6J1IFC3 (two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476430 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.5e-166
Identity = 311/355 (87.61%), Postives = 326/355 (91.83%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDIPRRKRRLRRTKSAPHAVSPHTEITCTSNVPATGPV 60
           MGSQE RQP+LPTSSNT   R IDIPR KRRLRRTKSAPH+ SPHTE T T    ATG V
Sbjct: 1   MGSQEVRQPMLPTSSNTFVTRSIDIPRSKRRLRRTKSAPHSGSPHTETTST----ATGLV 60

Query: 61  PRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTT 120
           PRSGL+FGNLHPSF RVALVLI+YLGIGTLC YLVR+QIKG KTNG+VDAIYFTIVTMTT
Sbjct: 61  PRSGLVFGNLHPSFIRVALVLIMYLGIGTLCVYLVRHQIKGGKTNGVVDAIYFTIVTMTT 120

Query: 121 VGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCD 180
           VGYGDLVPN+PS KLLACAF+FTGMA++GLIL+NAADYLVEKQEI LFKAFHMHQNG C 
Sbjct: 121 VGYGDLVPNTPSTKLLACAFMFTGMAVVGLILTNAADYLVEKQEIFLFKAFHMHQNGSCG 180

Query: 181 ITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240
            T++IDTNKARNKCIVVFL LLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS
Sbjct: 181 RTEKIDTNKARNKCIVVFLFLLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKS 240

Query: 241 FSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLD 300
           FSTRWGRLFAIFWILIS+ITLAQFFLYIA LNTERR+KSLVKWVL KKVT LDLE ADLD
Sbjct: 241 FSTRWGRLFAIFWILISSITLAQFFLYIAVLNTERRKKSLVKWVLSKKVTVLDLEAADLD 300

Query: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQLS 356
           DDGVVGAAEFVIYKLKEMGKITEDDISPVL EFENLDVDQSGTLSISDITLAQ S
Sbjct: 301 DDGVVGAAEFVIYKLKEMGKITEDDISPVLDEFENLDVDQSGTLSISDITLAQSS 351

[Close]

BLAST of HG10022397 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 404.4 bits (1038), Expect = 9.1e-113
Identity = 215/359 (59.89%), Postives = 268/359 (74.65%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDI-----PRRKRRLRRTKSAPHAVSPHTEITCTSNVP 60
           M S  AR PLLPT    +  +  ++       RKRRLRR++SAP     + +       P
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPP 60

Query: 61  ATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTI 120
              P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+VDA+YF I
Sbjct: 61  ---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCI 120

Query: 121 VTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQ 180
           VTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL +AFH+ Q
Sbjct: 121 VTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQ 180

Query: 181 N-GHCDITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTL 240
           + G  DI KE+ TNK R KC    L+L++  I GT+FLV +EK+  I AFYCVCST+TTL
Sbjct: 181 SFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTL 240

Query: 241 GYGDKSFSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDL 300
           GYGDKSF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKWVL +++T+ DL
Sbjct: 241 GYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDL 300

Query: 301 EVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQ 354
           E ADLD+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD D+SGTL+ SDI LAQ
Sbjct: 301 EAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQ 356

[Close]

BLAST of HG10022397 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 404.4 bits (1038), Expect = 9.1e-113
Identity = 215/359 (59.89%), Postives = 268/359 (74.65%), Query Frame = 0


Query: 1   MGSQEARQPLLPTSSNTSGKRLIDI-----PRRKRRLRRTKSAPHAVSPHTEITCTSNVP 60
           M S  AR PLLPT    +  +  ++       RKRRLRR++SAP     + +       P
Sbjct: 1   MSSDAARTPLLPTEKIDTMAQDFNLNSRTSSSRKRRLRRSRSAPRGDCMYNDDVKIDEPP 60

Query: 61  ATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTI 120
              P P    +F +L+P+ RRV + L +YL IGTLCFYLVR QI G KT+G+VDA+YF I
Sbjct: 61  ---PHPSKIPMFSDLNPNLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCI 120

Query: 121 VTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQ 180
           VTMTTVGYGDLVPNS +++LLACAFVF+GM L+G +LS AADYLVEKQE LL +AFH+ Q
Sbjct: 121 VTMTTVGYGDLVPNSSASRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQ 180

Query: 181 N-GHCDITKEIDTNKARNKCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTL 240
           + G  DI KE+ TNK R KC    L+L++  I GT+FLV +EK+  I AFYCVCST+TTL
Sbjct: 181 SFGPTDILKELHTNKLRYKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTL 240

Query: 241 GYGDKSFSTRWGRLFAIFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDL 300
           GYGDKSF++  GRLFA+FWIL S+I LAQFFLY+AELNTE +Q++LVKWVL +++T+ DL
Sbjct: 241 GYGDKSFNSEAGRLFAVFWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDL 300

Query: 301 EVADLDDDGVVGAAEFVIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDITLAQ 354
           E ADLD+DGVVGAAEF++YKLKEMGKI E DIS ++ EFE LD D+SGTL+ SDI LAQ
Sbjct: 301 EAADLDEDGVVGAAEFIVYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIVLAQ 356

[Close]

BLAST of HG10022397 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 192.2 bits (487), Expect = 7.1e-49
Identity = 117/331 (35.35%), Postives = 184/331 (55.59%), Query Frame = 0


Query: 30  RRLRRTKSAPHAVSPHTEITCTSNVPATGPVPRSGLIFGNLHPSFRRVALVLIIYLGIGT 89
           R L R+++AP  V      T         PV +S           R+   +LI+YL +G 
Sbjct: 82  RPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSKS---------IIRQAIFLLIVYLTLGV 141

Query: 90  LCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAKLLACAFVFTGMALIG 149
             +   R    G +T+ +VDA+YF IVTM T+GYGD+ P +P  K+ A  FV  G   + 
Sbjct: 142 SIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPWTKIFAVVFVLFGFGFLD 201

Query: 150 LILSNAADYLVEKQEILLF-----KAFHMHQNGHCDITKE--IDTNKA----RNKCIVVF 209
           ++LS   +Y+++ QE ++      +  H H + H    K+  ID  K     R K  +  
Sbjct: 202 ILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYIIDFEKGRMRIRMKVCLAL 261

Query: 210 LILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAIFWILIST 269
            +++L I  G + L  +E+L F+D+ Y    ++TT+GYGD++F T  GRLFA  W+L+ST
Sbjct: 262 CVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRAFKTLQGRLFAAVWLLVST 321

Query: 270 ITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEFVIYKLKEM 329
           + +A+ FLY+AE   +RR +  VK  L +++T  DL  AD    G +  +E+++ KLKEM
Sbjct: 322 LAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTYQHGFISKSEYIVLKLKEM 381

Query: 330 GKITEDDISPVLKEFENLDVDQSGTLSISDI 350
           GKIT+ DI  V+ +FE LD +Q G +++ D+
Sbjct: 382 GKITQKDIDQVVIQFEKLDPNQIGKITLPDL 403

[Close]

BLAST of HG10022397 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 191.8 bits (486), Expect = 9.3e-49
Identity = 103/279 (36.92%), Postives = 166/279 (59.50%), Query Frame = 0


Query: 75  RRVALVLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAK 134
           R+   +L++YL +G L ++L R      +T+ +VD +YF IVTM T+GYGD+ PNS   K
Sbjct: 151 RQAFALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTK 210

Query: 135 LLACAFVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCDITKEIDTNKARN-- 194
           L +  FV  G   I ++LS    Y+++ QE  +  +           +  ID  K R   
Sbjct: 211 LFSIMFVLVGFGFIDILLSGMVSYVLDLQESYMLDSAKRRDEPEKRRSYIIDVKKGRMRI 270

Query: 195 --KCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFA 254
             K  +   +++L I  G   +  +E++ ++D+FY    ++TT+GYGD++F T  GRLFA
Sbjct: 271 RLKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFA 330

Query: 255 IFWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEF 314
             W+L+ST+ +A+ FLY+AE   ++R +   K VLC+ ++      AD+D++G V  AE+
Sbjct: 331 AIWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEY 390

Query: 315 VIYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDI 350
           VIYKLKEM KIT+ DI P+ K+F+ LD   +G +++ D+
Sbjct: 391 VIYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDL 429

[Close]

BLAST of HG10022397 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 174.5 bits (441), Expect = 1.5e-43
Identity = 100/278 (35.97%), Postives = 165/278 (59.35%), Query Frame = 0


Query: 80  VLIIYLGIGTLCFYLVRYQIKGEKTNGIVDAIYFTIVTMTTVGYGDLVPNSPSAKLLACA 139
           +L++YL +G L ++L R     ++T+ +VDA+YF IVTM T+GYGD+ P+S   KL +  
Sbjct: 152 LLVVYLSLGVLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIF 211

Query: 140 FVFTGMALIGLILSNAADYLVEKQEILLFKAFHMHQNGHCDITKE----IDTNKARN--- 199
           FV  G   + ++LS    Y+++ QE  + +          D  K     ID  K R    
Sbjct: 212 FVLVGFGFMDILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRMRIR 271

Query: 200 -KCIVVFLILLLFIISGTVFLVTLEKLDFIDAFYCVCSTITTLGYGDKSFSTRWGRLFAI 259
            K  +   +++L +  G + +  +EK+ ++D+FY    ++TT+GYGD++F+T  GRL A 
Sbjct: 272 LKVGLALGVVVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAA 331

Query: 260 FWILISTITLAQFFLYIAELNTERRQKSLVKWVLCKKVTDLDLEVADLDDDGVVGAAEFV 319
            W+L+ST+ +A+  L++AE   ++R +   K VL + ++      AD+D +G V  AEFV
Sbjct: 332 MWLLVSTLAVARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKAEFV 391

Query: 320 IYKLKEMGKITEDDISPVLKEFENLDVDQSGTLSISDI 350
           IYKLK+M KITE DI+P+  +F+ LD   SG +++ D+
Sbjct: 392 IYKLKKMDKITEKDINPIGFQFDKLDRTNSGRITLLDL 429

The following BLAST results are available for this feature:

BLAST of HG10022397 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896135.1	4.8e-180	92.96	two-pore potassium channel 1 [Benincasa hispida]	[more]
TYK07941.1	4.0e-179	92.39	two-pore potassium channel 1 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008462994.1	4.5e-178	91.83	PREDICTED: two-pore potassium channel 1 isoform X1 [Cucumis melo]	[more]
XP_004150789.1	3.5e-175	91.27	two-pore potassium channel 1 [Cucumis sativus] >KGN44008.1 hypothetical protein ...	[more]
KAG7024969.1	6.0e-167	87.89	Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]

BLAST of HG10022397 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBL1	1.3e-111	59.89	Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2	[more]
Q850M0	8.9e-97	53.95	Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE...	[more]
Q8LIN5	5.8e-88	53.70	Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE...	[more]
Q9S6Z8	1.0e-47	35.35	Two-pore potassium channel 5 OS=Arabidopsis thaliana OX=3702 GN=TPK5 PE=1 SV=1	[more]
Q9SVV6	1.3e-47	36.92	Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1	[more]

BLAST of HG10022397 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C7V3	2.0e-179	92.39	Two-pore potassium channel 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CI53	2.2e-178	91.83	two-pore potassium channel 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501240 ...	[more]
A0A0A0K3G0	1.7e-175	91.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G098760 PE=3 SV=1	[more]
A0A6J1FCF3	1.1e-166	88.17	two-pore potassium channel 1 OS=Cucurbita moschata OX=3662 GN=LOC111442740 PE=3 ...	[more]
A0A6J1IFC3	2.5e-166	87.61	two-pore potassium channel 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476430 PE...	[more]

BLAST of HG10022397 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55630.1	9.1e-113	59.89	Outward rectifying potassium channel protein	[more]
AT5G55630.2	9.1e-113	59.89	Outward rectifying potassium channel protein	[more]
AT4G01840.1	7.1e-49	35.35	Ca2+ activated outward rectifying K+ channel 5	[more]
AT4G18160.1	9.3e-49	36.92	Ca2+ activated outward rectifying K+ channel 6	[more]
AT5G46370.1	1.5e-43	35.97	Ca2+ activated outward rectifying K+ channel 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003280	Two pore domain potassium channel	PRINTS	PR01333	2POREKCHANEL	coord: 116..144 score: 29.2 coord: 232..241 score: 45.91
IPR003280	Two pore domain potassium channel	PANTHER	PTHR11003	POTASSIUM CHANNEL, SUBFAMILY K	coord: 20..349
None	No IPR available	GENE3D	1.10.287.70		coord: 71..165 e-value: 1.1E-22 score: 81.7
None	No IPR available	GENE3D	1.10.287.70		coord: 177..283 e-value: 2.0E-20 score: 74.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 24..38
None	No IPR available	PANTHER	PTHR11003:SF270	TWO PORE POTASSIUM CHANNEL A	coord: 20..349
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 71..174
None	No IPR available	SUPERFAMILY	81324	Voltage-gated potassium channels	coord: 187..293
IPR013099	Potassium channel domain	PFAM	PF07885	Ion_trans_2	coord: 80..160 e-value: 3.2E-17 score: 62.3 coord: 200..271 e-value: 2.3E-15 score: 56.3
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 298..310
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 293..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022397.1	HG10022397.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030007	cellular potassium ion homeostasis
biological_process	GO:0071257	cellular response to electrical stimulus
biological_process	GO:0097623	potassium ion export across plasma membrane
biological_process	GO:1990573	potassium ion import across plasma membrane
biological_process	GO:0010029	regulation of seed germination
biological_process	GO:0010119	regulation of stomatal movement
biological_process	GO:0030322	stabilization of membrane potential
biological_process	GO:0071805	potassium ion transmembrane transport
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0009705	plant-type vacuole membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022841	potassium ion leak channel activity
molecular_function	GO:0005267	potassium channel activity