Homology
BLAST of HG10022309 vs. NCBI nr
Match:
XP_038889788.1 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Benincasa hispida])
HSP 1 Score: 2086.2 bits (5404), Expect = 0.0e+00
Identity = 1096/1179 (92.96%), Postives = 1116/1179 (94.66%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAINVYSI SPQ LWPPLS+P PSF CP YSSPPIQ SRF +P + Y TYP R
Sbjct: 1 MASPAINVYSI-SPQHLWPPLSHPLPSFICPHYSSPPIQVSRFCRHKP--VAFYSTYPLR 60
Query: 61 FRPSFQSPRSIFSQKSQLSDV----DEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDE 120
FRPSF SPRSI SQKSQLSDV DEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDE
Sbjct: 61 FRPSFPSPRSILSQKSQLSDVDEDEDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDE 120
Query: 121 VELSMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQA 180
VELSMEATEIS+APEEFKWQ+VEKLLGEV+EFG+GIIDVDELASVYNFRIDKFQRLAIQA
Sbjct: 121 VELSMEATEISSAPEEFKWQKVEKLLGEVREFGEGIIDVDELASVYNFRIDKFQRLAIQA 180
Query: 181 FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN 240
FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN
Sbjct: 181 FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN 240
Query: 241 VGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISR 300
VGLLTGDSAVNKDA VLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISR
Sbjct: 241 VGLLTGDSAVNKDAPVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISR 300
Query: 301 GTVWEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTK 360
GTVWEEI IHGKTELVTSSKRPVPLTWHFSTK
Sbjct: 301 GTVWEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSSKRPVPLTWHFSTK 360
Query: 361 TSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMS 420
TSLLPLLDEKGARMN RKLSLNYLQLHASGAKLYKDDGSRRR PKR GNEISYDN+SSMS
Sbjct: 361 TSLLPLLDEKGARMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRRGNEISYDNISSMS 420
Query: 421 RQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLD 480
RQATLSKNDINSIRRSNVPQVIDTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEGSNLLD
Sbjct: 421 RQATLSKNDINSIRRSNVPQVIDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGSNLLD 480
Query: 481 DCERSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV 540
DCERSEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV
Sbjct: 481 DCERSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV 540
Query: 541 VFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLL 600
VFATETLAAGINMPARTAVIASLSKRSNNGRTQLS NELLQMAGRAGRRGIDKKGHVVLL
Sbjct: 541 VFATETLAAGINMPARTAVIASLSKRSNNGRTQLSPNELLQMAGRAGRRGIDKKGHVVLL 600
Query: 601 QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLE 660
QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLE
Sbjct: 601 QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLE 660
Query: 661 EARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNE 720
EARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNE
Sbjct: 661 EARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNE 720
Query: 721 IAELQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIP 780
IA+LQEELRSEKRHRTELRKEME+QR+ ALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIP
Sbjct: 721 IAQLQEELRSEKRHRTELRKEMEAQRVFALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIP 780
Query: 781 AVLLGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIK 840
AVLLGNMDSSKL+NMFPADNSL+GTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIK
Sbjct: 781 AVLLGNMDSSKLVNMFPADNSLNGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIK 840
Query: 841 TVYKTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLN 900
TVYKTGFPNVALTKGDALPREIMR LLDKEGMKWEKLADSELGSLACMEGSLETWSWSLN
Sbjct: 841 TVYKTGFPNVALTKGDALPREIMRSLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLN 900
Query: 901 VPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLI 960
VPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKK+I RTEGFREYKKILDMA++I
Sbjct: 901 VPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKKIGRTEGFREYKKILDMASII 960
Query: 961 EDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIR 1020
EDKIRQLK RYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIR
Sbjct: 961 EDKIRQLKTRYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIR 1020
Query: 1021 GENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINF 1080
GENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFE SRTVINMINF
Sbjct: 1021 GENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEPSRTVINMINF 1080
Query: 1081 LEEQRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRR 1140
LEEQRNSL + QEKHGVNI CCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRR
Sbjct: 1081 LEEQRNSLEEFQEKHGVNISCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRR 1140
Query: 1141 TIDLLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
TIDLLAQIPKLPDIDPSLQSNAS+ASNVMNRPPISELAG
Sbjct: 1141 TIDLLAQIPKLPDIDPSLQSNASTASNVMNRPPISELAG 1175
BLAST of HG10022309 vs. NCBI nr
Match:
XP_008465717.2 (PREDICTED: LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Cucumis melo])
HSP 1 Score: 2047.3 bits (5303), Expect = 0.0e+00
Identity = 1081/1176 (91.92%), Postives = 1102/1176 (93.71%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLW-PPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPF 60
MA PAI+VYSI S Q L PPLSYPFPSF CP YSSP IQASRF +P L Y TYP
Sbjct: 1 MAIPAIDVYSIISLQHLCPPPLSYPFPSFVCPHYSSPSIQASRFCRHKP--LAFYSTYPV 60
Query: 61 RFRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVEL 120
RFRPSFQSPRSIFS+KSQLSDVDEDEDEDEDEDD+DDVAAEEYDSDALG FEQSYDEVEL
Sbjct: 61 RFRPSFQSPRSIFSEKSQLSDVDEDEDEDEDEDDEDDVAAEEYDSDALGEFEQSYDEVEL 120
Query: 121 SMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
SM+A+EIS APEEFKWQRVEKLLGEV+EFG+GIIDVDELASVYNFRIDKFQRLAIQAFLR
Sbjct: 121 SMDASEISNAPEEFKWQRVEKLLGEVREFGEGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
Query: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL
Sbjct: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
Query: 241 LTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTV 300
LTGDSAVNKDA VLIMTTEILRNMLYQSVGMA+SASGLFHVDVIVLDEVHYLSDISRGTV
Sbjct: 241 LTGDSAVNKDAPVLIMTTEILRNMLYQSVGMATSASGLFHVDVIVLDEVHYLSDISRGTV 300
Query: 301 WEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSL 360
WEEI IHGKTELVTSSKRPVPLTWHFSTKTSL
Sbjct: 301 WEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSSKRPVPLTWHFSTKTSL 360
Query: 361 LPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQA 420
LPLLDEKG RMN RKLSLNYLQLHASGAKLYKDDGS RR PKR GNEISYDN+ SMSRQA
Sbjct: 361 LPLLDEKGTRMN-RKLSLNYLQLHASGAKLYKDDGS-RRTPKRRGNEISYDNIGSMSRQA 420
Query: 421 TLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCE 480
TLSKNDINSIRRSNVPQVIDTLWQLK+KDMLPAVWFIFSRKGCDAAVQY++ SNLLDDCE
Sbjct: 421 TLSKNDINSIRRSNVPQVIDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYVDSSNLLDDCE 480
Query: 481 RSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
RSEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA
Sbjct: 481 RSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
Query: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTP 600
TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGID KGHVVLLQTP
Sbjct: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDXKGHVVLLQTP 600
Query: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEAR 660
YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETK FQA RTLEEAR
Sbjct: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKTFQAWRTLEEAR 660
Query: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE
Sbjct: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
Query: 721 LQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVL 780
LQEELRSEKRHRTELRKEMESQRI ALNSLLRNLGDG LPFLCLQYKDSEGVQHSIP VL
Sbjct: 721 LQEELRSEKRHRTELRKEMESQRIFALNSLLRNLGDGHLPFLCLQYKDSEGVQHSIPTVL 780
Query: 781 LGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
LGNMDSSKL NMFPADNSLSG ESNL I LEPG+ESSYYVALGSDNSWYLFTEKWIKTVY
Sbjct: 781 LGNMDSSKLGNMFPADNSLSGAESNLDITLEPGSESSYYVALGSDNSWYLFTEKWIKTVY 840
Query: 841 KTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
KTGFPNVALTKGDALPREIMR LLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV
Sbjct: 841 KTGFPNVALTKGDALPREIMRSLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
Query: 901 LNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDK 960
LNSLSENDELLQMSQSYMESLERYK QRNKVARLKKRI+RTEGFREYKKILDMANL+EDK
Sbjct: 901 LNSLSENDELLQMSQSYMESLERYKVQRNKVARLKKRISRTEGFREYKKILDMANLVEDK 960
Query: 961 IRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
IRQLK RYKRLSNRI+QIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN
Sbjct: 961 IRQLKTRYKRLSNRIEQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
Query: 1021 ELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEE 1080
ELWIAMVLRNKFLVQLKPTELA VCASLVSEGIKVRPGRNNSYIFE SRTVINMINFLEE
Sbjct: 1021 ELWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKVRPGRNNSYIFEPSRTVINMINFLEE 1080
Query: 1081 QRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
QRNSL DLQEKHGVNI CCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID
Sbjct: 1081 QRNSLEDLQEKHGVNISCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
Query: 1141 LLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LLAQIPKLPD+DPSLQSNAS+AS+VMNRPPISELAG
Sbjct: 1141 LLAQIPKLPDVDPSLQSNASTASDVMNRPPISELAG 1172
BLAST of HG10022309 vs. NCBI nr
Match:
XP_022150310.1 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Momordica charantia])
HSP 1 Score: 2021.9 bits (5237), Expect = 0.0e+00
Identity = 1051/1175 (89.45%), Postives = 1102/1175 (93.79%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAIN YSI +P+ LWPPLSYPFP+F CP S+PPIQ SRF L+P LTI YP R
Sbjct: 1 MASPAINAYSI-APKHLWPPLSYPFPTFICPHCSTPPIQTSRFCRLKP--LTICSPYPVR 60
Query: 61 FRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVELS 120
FRPSF SPRSIFS+KSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEV+LS
Sbjct: 61 FRPSFHSPRSIFSEKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVDLS 120
Query: 121 MEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLRG 180
+E TEIST PE+FKWQRVEKLL EV+EFG+ I+DVDELAS+YNFRIDKFQRLA+QAFLRG
Sbjct: 121 IETTEISTPPEDFKWQRVEKLLSEVREFGEEIVDVDELASIYNFRIDKFQRLAVQAFLRG 180
Query: 181 SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGLL 240
SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFG++NVGLL
Sbjct: 181 SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGENNVGLL 240
Query: 241 TGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW 300
TGDSAVNKDA +LIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW
Sbjct: 241 TGDSAVNKDAQILIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW 300
Query: 301 EEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSLL 360
EEI IHG+TELVTSSKRPVPLTWHFSTKTSLL
Sbjct: 301 EEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGQTELVTSSKRPVPLTWHFSTKTSLL 360
Query: 361 PLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQAT 420
PLLDEKG RMN RKLSLNYLQLHASGAKLYKDDGSRRR PKRHGNEISYD+ SSMSRQAT
Sbjct: 361 PLLDEKGTRMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRHGNEISYDSTSSMSRQAT 420
Query: 421 LSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCER 480
LSKNDIN+IRRSNVPQV+DTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEG NLLD+CER
Sbjct: 421 LSKNDINTIRRSNVPQVMDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGRNLLDECER 480
Query: 481 SEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT 540
SEVELALR+FRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT
Sbjct: 481 SEVELALRRFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT 540
Query: 541 ETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPY 600
ETLAAGINMPAR+AVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGID+KGHVVL+QTPY
Sbjct: 541 ETLAAGINMPARSAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDQKGHVVLIQTPY 600
Query: 601 EGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEARK 660
EGAEECCK+LFAGIEPLVSQFTASYGMVLNLLAGAKVTH+SE+DETKAF AGRTLEEARK
Sbjct: 601 EGAEECCKILFAGIEPLVSQFTASYGMVLNLLAGAKVTHSSEIDETKAFLAGRTLEEARK 660
Query: 661 LVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAEL 720
LVEQSFGNYVGSNVMLAAKEELVKIEKEI+MLNLEIT+EAIDRKSRKFLS+VAYNEIAEL
Sbjct: 661 LVEQSFGNYVGSNVMLAAKEELVKIEKEIKMLNLEITEEAIDRKSRKFLSEVAYNEIAEL 720
Query: 721 QEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVLL 780
QEELRSEKRHRTELRKEME QRI AL SLL+NLGDG LPFLCLQYKDSEGVQHS+PAVLL
Sbjct: 721 QEELRSEKRHRTELRKEMELQRIYALKSLLQNLGDGHLPFLCLQYKDSEGVQHSVPAVLL 780
Query: 781 GNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVYK 840
GNMDSSK NMFP DN+LS ESNLGINL+PGAESSYYVALGSDNSWYLFTEKWI+TVYK
Sbjct: 781 GNMDSSKFNNMFPVDNALSAAESNLGINLDPGAESSYYVALGSDNSWYLFTEKWIRTVYK 840
Query: 841 TGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPVL 900
TGFPNVALTKGDALPRE+MR LLDKEGM WEKLADSELGSL+CMEGSLETWSWSLNVPVL
Sbjct: 841 TGFPNVALTKGDALPRELMRTLLDKEGMXWEKLADSELGSLSCMEGSLETWSWSLNVPVL 900
Query: 901 NSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDKI 960
NSLSENDELLQMS SYMESLERYKEQRN+VARLKKRIARTEGFREYKK+LDMAN+IEDKI
Sbjct: 901 NSLSENDELLQMSSSYMESLERYKEQRNRVARLKKRIARTEGFREYKKVLDMANIIEDKI 960
Query: 961 RQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE 1020
RQLKAR KRLSNRI+QIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE
Sbjct: 961 RQLKARSKRLSNRIEQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE 1020
Query: 1021 LWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEEQ 1080
LWIAMVLRNKFLVQLKPTELA VCASLVSEGIK+RPGRNNSYIFE S TV+NMI+FLEEQ
Sbjct: 1021 LWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKIRPGRNNSYIFEPSTTVMNMIDFLEEQ 1080
Query: 1081 RNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL 1140
R+SLL LQEKHGV+IPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL
Sbjct: 1081 RSSLLHLQEKHGVDIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL 1140
Query: 1141 LAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LAQIPKLPDIDPSLQSNAS+ASNVMNRPPISELAG
Sbjct: 1141 LAQIPKLPDIDPSLQSNASTASNVMNRPPISELAG 1171
BLAST of HG10022309 vs. NCBI nr
Match:
XP_011655295.1 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Cucumis sativus] >KGN51183.1 hypothetical protein Csa_009242 [Cucumis sativus])
HSP 1 Score: 2016.1 bits (5222), Expect = 0.0e+00
Identity = 1067/1176 (90.73%), Postives = 1092/1176 (92.86%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLW-PPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPF 60
MA PAINVYSI S Q L PPLSYPFPSF P YS ASRF +P L Y T P
Sbjct: 1 MAIPAINVYSIISLQHLCPPPLSYPFPSFLTPHYS-----ASRFCPHKP--LAFYSTRPV 60
Query: 61 RFRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVEL 120
FRPSF SPRSIFS+KSQLSDVDEDEDEDEDEDD+DDVAAEEYDSDALGGFEQSYDEVEL
Sbjct: 61 PFRPSFHSPRSIFSEKSQLSDVDEDEDEDEDEDDEDDVAAEEYDSDALGGFEQSYDEVEL 120
Query: 121 SMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
SM+ +EIS A +EFKWQRVEKLLGEV+EFG+GIIDVDELASVYNFRIDKFQRLA+QAFLR
Sbjct: 121 SMDTSEISNASQEFKWQRVEKLLGEVREFGEGIIDVDELASVYNFRIDKFQRLAVQAFLR 180
Query: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL
Sbjct: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
Query: 241 LTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTV 300
LTGDSAVNKDA VLIMTTEILRNMLYQSVGMA+SASGLFHVDVIVLDEVHYLSDISRGTV
Sbjct: 241 LTGDSAVNKDAPVLIMTTEILRNMLYQSVGMATSASGLFHVDVIVLDEVHYLSDISRGTV 300
Query: 301 WEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSL 360
WEEI IHGKTELVTSSKRPVPLTWHFSTKTSL
Sbjct: 301 WEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSSKRPVPLTWHFSTKTSL 360
Query: 361 LPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQA 420
LPLLDEKGARMN RKLSLNYLQL+ASG K KDDGSRRR PKR GNEISYDN+ SMSRQA
Sbjct: 361 LPLLDEKGARMN-RKLSLNYLQLNASGDKFSKDDGSRRRTPKRRGNEISYDNIGSMSRQA 420
Query: 421 TLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCE 480
TLSKNDINSIRRSNVPQVIDTLWQLK+KDMLPAVWFIFSRKGCDAAVQYI+ SNLLDDCE
Sbjct: 421 TLSKNDINSIRRSNVPQVIDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIDSSNLLDDCE 480
Query: 481 RSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
RSEVELALRKFRIQFPDA+RESA+KGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA
Sbjct: 481 RSEVELALRKFRIQFPDAVRESAIKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
Query: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTP 600
TETLAAGINMPARTAVIASLSKRSNNGRT LS NELLQMAGRAGRRGIDKKGHVVLLQTP
Sbjct: 541 TETLAAGINMPARTAVIASLSKRSNNGRTHLSPNELLQMAGRAGRRGIDKKGHVVLLQTP 600
Query: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEAR 660
YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQA RTLEEAR
Sbjct: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAWRTLEEAR 660
Query: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSD+AYNEIAE
Sbjct: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDMAYNEIAE 720
Query: 721 LQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVL 780
LQEELR EKRHRTELRKEMESQRICALNSLLRNLGDG LPFLCLQYKDSEGVQHSIP VL
Sbjct: 721 LQEELRLEKRHRTELRKEMESQRICALNSLLRNLGDGHLPFLCLQYKDSEGVQHSIPTVL 780
Query: 781 LGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
LGNMDSSKL NMFPAD+SLSG ESNLGI LEPGAESSYYVALGSDNSWYLFTEKWIKTVY
Sbjct: 781 LGNMDSSKLGNMFPADSSLSGAESNLGITLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
Query: 841 KTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
KTGFPNVAL+KGDALPREIMR LLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV
Sbjct: 841 KTGFPNVALSKGDALPREIMRSLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
Query: 901 LNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDK 960
LNSLSENDELLQMSQSYMESL+RYK QRNKVARLKKRI++TEGFREYKKILDMANLIEDK
Sbjct: 901 LNSLSENDELLQMSQSYMESLDRYKVQRNKVARLKKRISKTEGFREYKKILDMANLIEDK 960
Query: 961 IRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
IRQLK RYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN
Sbjct: 961 IRQLKTRYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
Query: 1021 ELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEE 1080
ELWIAMVLRNKFLVQLKPTELA VCASLVSEGIKVRPGRNNSYIFE SRTVINMINFLEE
Sbjct: 1021 ELWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKVRPGRNNSYIFEPSRTVINMINFLEE 1080
Query: 1081 QRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
QRNSL DLQEKHGVNI CCLDSQFSGMVEAWASGLTWRE+MMDCAMDEGDLARLLRRTID
Sbjct: 1081 QRNSLEDLQEKHGVNISCCLDSQFSGMVEAWASGLTWREIMMDCAMDEGDLARLLRRTID 1140
Query: 1141 LLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LLAQIPKLPDIDPSLQ NAS+AS+VMNRPPISELAG
Sbjct: 1141 LLAQIPKLPDIDPSLQRNASTASDVMNRPPISELAG 1168
BLAST of HG10022309 vs. NCBI nr
Match:
KAG7029994.1 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1997.2 bits (5173), Expect = 0.0e+00
Identity = 1048/1183 (88.59%), Postives = 1091/1183 (92.22%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAINV+SI SPQ LWPPLSYPFPS C + SSPPIQA RF L+P L I+ +P
Sbjct: 1 MASPAINVFSIISPQHLWPPLSYPFPSSLCSRCSSPPIQAPRFCRLKP--LPIFSPFPVP 60
Query: 61 FRPSFQSPRSIFSQKSQLSDV--------DEDEDEDEDEDDDDDVAAEEYDSDALGGFEQ 120
FRPS +SPRSIFS+K QLSDV DEDEDEDEDEDDDDDVAAEEYD DA G EQ
Sbjct: 61 FRPSVRSPRSIFSEKPQLSDVDEDEDEDEDEDEDEDEDEDDDDDVAAEEYDIDAFGELEQ 120
Query: 121 SYDEVELSMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRL 180
YDEVELSMEATEIS APEEFK QRVEKLLGEV+EFG+GI+DVDELASVYNFRIDKFQR
Sbjct: 121 RYDEVELSMEATEISIAPEEFKSQRVEKLLGEVREFGEGIVDVDELASVYNFRIDKFQRQ 180
Query: 181 AIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF 240
AI+AFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF
Sbjct: 181 AIEAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF 240
Query: 241 GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLS 300
GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASS SGLFHVDVIVLDEVHYLS
Sbjct: 241 GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSESGLFHVDVIVLDEVHYLS 300
Query: 301 DISRGTVWEEI-----------------------------IHGKTELVTSSKRPVPLTWH 360
DISRGTVWEEI IHGKTELVTS+KRPVPLTWH
Sbjct: 301 DISRGTVWEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSTKRPVPLTWH 360
Query: 361 FSTKTSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNV 420
FSTKTSLLPLLDEKG MN RKLSLNYLQLHASGAKLYKDDGSRRR PKRHGNEIS D
Sbjct: 361 FSTKTSLLPLLDEKGTHMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRHGNEISNDRT 420
Query: 421 SSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGS 480
SS+SRQATLSKNDINSIRRSNVPQV+DTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEG
Sbjct: 421 SSLSRQATLSKNDINSIRRSNVPQVVDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGK 480
Query: 481 NLLDDCERSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG 540
NLLDDCERSEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG
Sbjct: 481 NLLDDCERSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG 540
Query: 541 LVKVVFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGH 600
LVKVVFATETLAAGINMPARTAVIASLSKRSN+GRTQL+SNELLQMAGRAGRRGIDKKGH
Sbjct: 541 LVKVVFATETLAAGINMPARTAVIASLSKRSNSGRTQLTSNELLQMAGRAGRRGIDKKGH 600
Query: 601 VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAG 660
VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMD TKAFQAG
Sbjct: 601 VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDGTKAFQAG 660
Query: 661 RTLEEARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDV 720
RTLEEARKLVEQSFGNYVGSNVM+AAKEELVKIEKEIE+LNLEITDEAID+K R+ +SDV
Sbjct: 661 RTLEEARKLVEQSFGNYVGSNVMIAAKEELVKIEKEIEVLNLEITDEAIDKKCRRLMSDV 720
Query: 721 AYNEIAELQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQ 780
AYNE+ ELQE LRS+KR RTELRKEME QRICAL SLL+NLGDGQLPFLCLQYKDSEGVQ
Sbjct: 721 AYNELVELQEGLRSDKRQRTELRKEMELQRICALKSLLQNLGDGQLPFLCLQYKDSEGVQ 780
Query: 781 HSIPAVLLGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTE 840
HSIPAVL GNMDSSKLINMFPADNSLSG E NLG NL+PGAESSYYVALGSDNSWYLFTE
Sbjct: 781 HSIPAVLFGNMDSSKLINMFPADNSLSGAEPNLGKNLDPGAESSYYVALGSDNSWYLFTE 840
Query: 841 KWIKTVYKTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWS 900
KWIKTVYKTGFPNVALTKGD LPREIMR LLDKEGMKWEKLADSELGSL+CMEGSLETWS
Sbjct: 841 KWIKTVYKTGFPNVALTKGDPLPREIMRTLLDKEGMKWEKLADSELGSLSCMEGSLETWS 900
Query: 901 WSLNVPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDM 960
WSLNVPVLNSLSENDELLQMS+SYMESL RYKEQRNKV+R KKRI+RT+GFREYKKILDM
Sbjct: 901 WSLNVPVLNSLSENDELLQMSESYMESLTRYKEQRNKVSRFKKRISRTKGFREYKKILDM 960
Query: 961 ANLIEDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETA 1020
AN+IEDKIRQLKARY+RLSNRIQQIEPSGWKEFLQISNVIHEIRALDIN+HVMFPLGETA
Sbjct: 961 ANIIEDKIRQLKARYERLSNRIQQIEPSGWKEFLQISNVIHEIRALDINSHVMFPLGETA 1020
Query: 1021 AAIRGENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVIN 1080
AAIRGENELWIA+VLRNKFLVQLKPTELAGVCASLVSEGIK+RPGR+N+YIFE SRTVIN
Sbjct: 1021 AAIRGENELWIAVVLRNKFLVQLKPTELAGVCASLVSEGIKIRPGRDNNYIFEPSRTVIN 1080
Query: 1081 MINFLEEQRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLAR 1140
M+NFLEEQRNSLLDLQEKHGVNIPC LDSQFSGMVEAWASGLTWRE+MMDCAMDEGDLAR
Sbjct: 1081 MVNFLEEQRNSLLDLQEKHGVNIPCSLDSQFSGMVEAWASGLTWREIMMDCAMDEGDLAR 1140
Query: 1141 LLRRTIDLLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LLRRTIDLLAQIPKLPDIDPSLQ NAS+AS+VMNR PISELAG
Sbjct: 1141 LLRRTIDLLAQIPKLPDIDPSLQRNASTASDVMNRSPISELAG 1180
BLAST of HG10022309 vs. ExPASy Swiss-Prot
Match:
B9DFG3 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISE2 PE=1 SV=2)
HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 776/1167 (66.50%), Postives = 939/1167 (80.46%), Query Frame = 0
Query: 23 YPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFRFRPSFQSPRSIFSQKSQLSDVD 82
+ FPS SP ++ L P L+ T S+ +SQL + +
Sbjct: 18 FHFPSLHRSLSHSPNFSFTKSLILNPNHLSFKSTL-----------NSLSPSQSQLYEEE 77
Query: 83 EDEDEDEDEDDDDDVAAEEYD--SDALGGFEQSYD--EVELSME-ATEISTAPEEFKWQR 142
+DE+E+E+++DDDD AA+EYD SD + + D E E S++ TE + EF+WQR
Sbjct: 78 DDEEEEEEDEDDDDEAADEYDNISDEIRNSDDDDDDEETEFSVDLPTESARERVEFRWQR 137
Query: 143 VEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIA 202
VEKL V++FG +ID+DEL S+Y+FRIDKFQRLAI+AFLRGSSVVVSAPTSSGKTLIA
Sbjct: 138 VEKLRSLVRDFGVEMIDIDELISIYDFRIDKFQRLAIEAFLRGSSVVVSAPTSSGKTLIA 197
Query: 203 EAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTT 262
EAAAV+TVA+ RRLFYTTPLKALSNQKFREFRETFGD NVGLLTGDSA+NKDA ++IMTT
Sbjct: 198 EAAAVSTVAKGRRLFYTTPLKALSNQKFREFRETFGDDNVGLLTGDSAINKDAQIVIMTT 257
Query: 263 EILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVWEEI-------------- 322
EILRNMLYQSVGMASS +GLFHVD IVLDEVHYLSDISRGTVWEEI
Sbjct: 258 EILRNMLYQSVGMASSGTGLFHVDAIVLDEVHYLSDISRGTVWEEIVIYCPKEVQLICLS 317
Query: 323 ---------------IHGKTELVTSSKRPVPLTWHFSTKTSLLPLLDEKGARMNGRKLSL 382
IHGKTELVTS++RPVPLTW+FSTK SLLPLLDEKG +N RKLSL
Sbjct: 318 ATVANPDELAGWIGEIHGKTELVTSTRRPVPLTWYFSTKHSLLPLLDEKGINVN-RKLSL 377
Query: 383 NYLQLHASGAKLY-KDDGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSIRRSNVPQ 442
NYLQL AS A+ DDG R+R+ K+ G + SY+N+ +++ LSKN+IN IRRS VPQ
Sbjct: 378 NYLQLSASEARFRDDDDGYRKRRSKKRGGDTSYNNLVNVT-DYPLSKNEINKIRRSQVPQ 437
Query: 443 VIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCERSEVELALRKFRIQFPD 502
+ DTLW L+ K+MLPA+WFIF+R+GCDAAVQY+E LLDDCE+SEVELAL+KFR+ +PD
Sbjct: 438 ISDTLWHLQGKNMLPAIWFIFNRRGCDAAVQYVENFQLLDDCEKSEVELALKKFRVLYPD 497
Query: 503 ALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI 562
A+RESA KGLL+G+AAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI
Sbjct: 498 AVRESAEKGLLRGIAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI 557
Query: 563 ASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPYEGAEECCKLLFAGIEP 622
+SLSK++ N R +L NEL QMAGRAGRRGID+KG+ VL+QT +EGAEECCKL+FAG++P
Sbjct: 558 SSLSKKAGNERIELGPNELYQMAGRAGRRGIDEKGYTVLVQTAFEGAEECCKLVFAGVKP 617
Query: 623 LVSQFTASYGMVLNLLAGAKVTHTSEMDET-KAFQAGRTLEEARKLVEQSFGNYVGSNVM 682
LVSQFTASYGMVLNL+AG+KVT S E K QAGR+LEEA+KLVE+SFGNYV SNV
Sbjct: 618 LVSQFTASYGMVLNLVAGSKVTRKSSGTEAGKVLQAGRSLEEAKKLVEKSFGNYVSSNVT 677
Query: 683 LAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAELQEELRSEKRHRTELR 742
+AAK+EL +I+ +IE+L+ EI+DEAID+KSRK LS Y EI L+EELR EKR R E R
Sbjct: 678 VAAKQELAEIDNKIEILSSEISDEAIDKKSRKLLSARDYKEITVLKEELREEKRKRAEQR 737
Query: 743 KEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVLLGNMDS---SKLINMF 802
+ ME +R AL LL+ + +G LPF+CL++KDSEG + S+PAV LG++DS SKL M
Sbjct: 738 RRMELERFLALKPLLKGMEEGNLPFICLEFKDSEGREQSVPAVYLGHIDSFQGSKLQKMM 797
Query: 803 PADNS--LSGTESNLGINL--EPGAESSYYVALGSDNSWYLFTEKWIKTVYKTGFPNVAL 862
D S L+ E L + +P + SYYVALGSDNSWYLFTEKW++TVY+TGFPN+AL
Sbjct: 798 SLDESFALNLIEDELAADEPGKPNVKPSYYVALGSDNSWYLFTEKWVRTVYRTGFPNIAL 857
Query: 863 TKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPVLNSLSENDE 922
GDALPREIM++LLDK M+W+KLA+SELGSL +EGSLETWSWSLNVPVL+SLS+ DE
Sbjct: 858 ALGDALPREIMKNLLDKADMQWDKLAESELGSLWRLEGSLETWSWSLNVPVLSSLSDEDE 917
Query: 923 LLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDKIRQLKARYK 982
+L MS+ Y + ++YKEQR+K++RLKK+++R+EGFREYKKIL+ ANL +K+++LKAR +
Sbjct: 918 VLHMSEEYDNAAQKYKEQRSKISRLKKKMSRSEGFREYKKILENANLTVEKMKRLKARSR 977
Query: 983 RLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENELWIAMVLR 1042
RL NR++QIEPSGWK+F++ISNVIHE RALDINTH++FPLGETAAAIRGENELW+AMVLR
Sbjct: 978 RLINRLEQIEPSGWKDFMRISNVIHESRALDINTHLIFPLGETAAAIRGENELWLAMVLR 1037
Query: 1043 NKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEEQRNSLLDLQ 1102
NK LV LKP +LAGVCASLVSEGIKVRP R+N+YI+E S TV++M+NFLE+QR+SL+ LQ
Sbjct: 1038 NKALVDLKPPQLAGVCASLVSEGIKVRPWRDNNYIYEPSDTVVDMVNFLEDQRSSLIKLQ 1097
Query: 1103 EKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDLLAQIPKLP 1147
EKH V IPCCLD QFSGMVEAWASGL+W+EMMM+CAMDEGDLARLLRRTIDLLAQIPKLP
Sbjct: 1098 EKHEVMIPCCLDVQFSGMVEAWASGLSWKEMMMECAMDEGDLARLLRRTIDLLAQIPKLP 1157
BLAST of HG10022309 vs. ExPASy Swiss-Prot
Match:
Q9ZBD8 (Probable helicase HelY OS=Mycobacterium leprae (strain TN) OX=272631 GN=helY PE=3 SV=1)
HSP 1 Score: 261.5 bits (667), Expect = 4.3e-68
Identity = 194/525 (36.95%), Postives = 260/525 (49.52%), Query Frame = 0
Query: 164 FRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQ 223
F +D FQ+ A A RG V+V APT +GKT++ E A +A + FYTTPLKALSNQ
Sbjct: 16 FSLDDFQQRACAALERGHGVLVCAPTGAGKTVVGEFAVHLALAAGGKCFYTTPLKALSNQ 75
Query: 224 KFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVI 283
K+ + +G + +GLLTGD +VN D+ V++MTTE+LRNMLY + + GL HV
Sbjct: 76 KYTDLTARYGRNRIGLLTGDQSVNGDSPVVVMTTEVLRNMLYAD---SFALQGLSHV--- 135
Query: 284 VLDEVHYLSDISRGTVWEEII-----------------------------HGKTELVTSS 343
V+DEVH+++D RG VWEE+I G T +V
Sbjct: 136 VMDEVHFIADRMRGPVWEEVILHLPDDVRMVSLSATVSNAEEFGGWVQTVRGDTTVVVDE 195
Query: 344 KRPVPLTWHFSTKTSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRH 403
RPVPL H L L D + ++ ++ N L+ H + + D S R P+R
Sbjct: 196 HRPVPLWQHVLVGKRLFDLFD-YDSNVDQSPVNPNLLR-HIAHCR-EADRMSDWRNPRRR 255
Query: 404 GNEISYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCD 463
R + + S+ R V ++D A+ +LPA+ F+FSR GCD
Sbjct: 256 A-----------GRGSGVRPRFYRSLARPEVIAILD------AEGLLPAITFVFSRFGCD 315
Query: 464 AAVQYIEGSNLLDDCERSEVELALRKFRIQFPDAL---------RESAV-------KGLL 523
AAVQ C RS + L + R Q + + + AV +GLL
Sbjct: 316 AAVQ---------QCLRSPLRLTSEEERAQIAEVIDHRCGDLADADLAVLGYYEWREGLL 375
Query: 524 QGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVIASLSKRSNNGR 583
+G+AAHHAG LP ++ +EELF GLVK VFATETLA GINMPART V+ L K +
Sbjct: 376 RGLAAHHAGMLPAFRHAVEELFTAGLVKAVFATETLALGINMPARTVVLERLVKFNGKQH 435
Query: 584 TQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPYE---GAEECCKLLFAGIEPLVSQFTAS 641
L+ E Q+ GRAGRRGID +GH V++ P E G L A PL S F S
Sbjct: 436 VPLTPGEYTQLTGRAGRRGIDVEGHAVVIWHPSEDTSGPSAVAGLASARTFPLRSSFVPS 487
BLAST of HG10022309 vs. ExPASy Swiss-Prot
Match:
P9WMR0 (Probable helicase HelY OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=helY PE=3 SV=1)
HSP 1 Score: 251.1 bits (640), Expect = 5.9e-65
Identity = 204/614 (33.22%), Postives = 286/614 (46.58%), Query Frame = 0
Query: 152 IIDVDELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRL 211
+ ++D + F +D FQ+ A A RG V+V APT +GKT++ E A +A +
Sbjct: 4 LAELDRFTAELPFSLDDFQQRACSALERGHGVLVCAPTGAGKTVVGEFAVHLALAAGSKC 63
Query: 212 FYTTPLKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMA 271
FYTTPLKALSNQK + +G +GLLTGD +VN +A V++MTTE+LRNMLY
Sbjct: 64 FYTTPLKALSNQKHTDLTARYGRDQIGLLTGDLSVNGNAPVVVMTTEVLRNMLY------ 123
Query: 272 SSASGLFHVDVIVLDEVHYLSDISRGTVWEEII--------------------------- 331
+ + L + +V+DEVH+L+D RG VWEE+I
Sbjct: 124 ADSPALQGLSYVVMDEVHFLADRMRGPVWEEVILQLPDDVRVVSLSATVSNAEEFGGWIQ 183
Query: 332 --HGKTELVTSSKRPVPLTWHFSTKTSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYK 391
G T +V RPVPL H + L D + G+ L H + + +
Sbjct: 184 TVRGDTTVVVDEHRPVPLWQHVLVGKRMFDLFDYRIGEAEGQPQVNRELLRHIAHRR--E 243
Query: 392 DDGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLP 451
D +P+R G+ R P+VI +L A+ +LP
Sbjct: 244 ADRMADWQPRRRGS------------------GRPGFYRPPGRPEVIA---KLDAEGLLP 303
Query: 452 AVWFIFSRKGCDAAVQYIEGSNLLDDCERSEVELALRKFRIQFPDAL---------RESA 511
A+ F+FSR GCDAAV C RS + L + R + + + + A
Sbjct: 304 AITFVFSRAGCDAAV---------TQCLRSPLRLTSEEERARIAEVIDHRCGDLADSDLA 363
Query: 512 V-------KGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAV 571
V +GLL+G+AAHHAG LP ++ +EELF GLVK VFATETLA GINMPART V
Sbjct: 364 VLGYYEWREGLLRGLAAHHAGMLPAFRHTVEELFTAGLVKAVFATETLALGINMPARTVV 423
Query: 572 IASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPYEGAEECCKLLFAGIE 631
+ L K + L+ E Q+ GRAGRRGID +GH V++ P E L
Sbjct: 424 LERLVKFNGEQHMPLTPGEYTQLTGRAGRRGIDVEGHAVVIWHPEIEPSEVAGLASTRTF 483
Query: 632 PLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEARKLVEQSFGNYVGSNVM 691
PL S F SY M +NL+ + G ++A +L+EQSF Y
Sbjct: 484 PLRSSFAPSYNMTINLV----------------HRMGP--QQAHRLLEQSFAQY------ 543
Query: 692 LAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAELQEELRSEKRHRTELR 721
A +V + + IE N + + A + SD E A L+ R + R + R
Sbjct: 544 -QADRSVVGLVRGIERGNRILGEIAAELGG----SDAPILEYARLR--ARVSELERAQAR 548
BLAST of HG10022309 vs. ExPASy Swiss-Prot
Match:
P9WMR1 (Probable helicase HelY OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=helY PE=1 SV=1)
HSP 1 Score: 251.1 bits (640), Expect = 5.9e-65
Identity = 204/614 (33.22%), Postives = 286/614 (46.58%), Query Frame = 0
Query: 152 IIDVDELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRL 211
+ ++D + F +D FQ+ A A RG V+V APT +GKT++ E A +A +
Sbjct: 4 LAELDRFTAELPFSLDDFQQRACSALERGHGVLVCAPTGAGKTVVGEFAVHLALAAGSKC 63
Query: 212 FYTTPLKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMA 271
FYTTPLKALSNQK + +G +GLLTGD +VN +A V++MTTE+LRNMLY
Sbjct: 64 FYTTPLKALSNQKHTDLTARYGRDQIGLLTGDLSVNGNAPVVVMTTEVLRNMLY------ 123
Query: 272 SSASGLFHVDVIVLDEVHYLSDISRGTVWEEII--------------------------- 331
+ + L + +V+DEVH+L+D RG VWEE+I
Sbjct: 124 ADSPALQGLSYVVMDEVHFLADRMRGPVWEEVILQLPDDVRVVSLSATVSNAEEFGGWIQ 183
Query: 332 --HGKTELVTSSKRPVPLTWHFSTKTSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYK 391
G T +V RPVPL H + L D + G+ L H + + +
Sbjct: 184 TVRGDTTVVVDEHRPVPLWQHVLVGKRMFDLFDYRIGEAEGQPQVNRELLRHIAHRR--E 243
Query: 392 DDGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLP 451
D +P+R G+ R P+VI +L A+ +LP
Sbjct: 244 ADRMADWQPRRRGS------------------GRPGFYRPPGRPEVIA---KLDAEGLLP 303
Query: 452 AVWFIFSRKGCDAAVQYIEGSNLLDDCERSEVELALRKFRIQFPDAL---------RESA 511
A+ F+FSR GCDAAV C RS + L + R + + + + A
Sbjct: 304 AITFVFSRAGCDAAV---------TQCLRSPLRLTSEEERARIAEVIDHRCGDLADSDLA 363
Query: 512 V-------KGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAV 571
V +GLL+G+AAHHAG LP ++ +EELF GLVK VFATETLA GINMPART V
Sbjct: 364 VLGYYEWREGLLRGLAAHHAGMLPAFRHTVEELFTAGLVKAVFATETLALGINMPARTVV 423
Query: 572 IASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPYEGAEECCKLLFAGIE 631
+ L K + L+ E Q+ GRAGRRGID +GH V++ P E L
Sbjct: 424 LERLVKFNGEQHMPLTPGEYTQLTGRAGRRGIDVEGHAVVIWHPEIEPSEVAGLASTRTF 483
Query: 632 PLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEARKLVEQSFGNYVGSNVM 691
PL S F SY M +NL+ + G ++A +L+EQSF Y
Sbjct: 484 PLRSSFAPSYNMTINLV----------------HRMGP--QQAHRLLEQSFAQY------ 543
Query: 692 LAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAELQEELRSEKRHRTELR 721
A +V + + IE N + + A + SD E A L+ R + R + R
Sbjct: 544 -QADRSVVGLVRGIERGNRILGEIAAELGG----SDAPILEYARLR--ARVSELERAQAR 548
BLAST of HG10022309 vs. ExPASy Swiss-Prot
Match:
O59801 (Putative ATP-dependent RNA helicase C550.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC550.03c PE=3 SV=1)
HSP 1 Score: 250.4 bits (638), Expect = 1.0e-64
Identity = 178/570 (31.23%), Postives = 287/570 (50.35%), Query Frame = 0
Query: 155 VDELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYT 214
V E+A + F +D FQ+ AI G SV V+A TS+GKT++AE A + YT
Sbjct: 270 VPEMALDFPFELDNFQKEAIYHLEMGDSVFVAAHTSAGKTVVAEYAIALAQKHMTKAIYT 329
Query: 215 TPLKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSA 274
+P+KALSNQKFR+F+ F D VG+LTGD VN + L+MTTEILR+MLY+ +
Sbjct: 330 SPIKALSNQKFRDFKHKFED--VGILTGDVQVNPEGSCLLMTTEILRSMLYRGADL---- 389
Query: 275 SGLFHVDVIVLDEVHYLSDISRGTVWEEII-------------------------HGKTE 334
+ V+ ++ DEVHY++D+ RG VWEE+I G+T+
Sbjct: 390 --IRDVEFVIFDEVHYVNDLERGVVWEEVIIMLPPHVTLILLSATVPNTKEFASWVGRTK 449
Query: 335 -----LVTSSKRPVPLTWHFSTKTSLLPLLDEKGA-RMNGRKLSLNYLQLHASGAKLYKD 394
++++ KRPVPL + K ++ ++D+ G M+G K + + L+ +
Sbjct: 450 KKNIYVISTLKRPVPLEHYLWVKQNMFKIVDQHGRFLMDGYKSANDALKKPDKPVIAKDN 509
Query: 395 DGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPA 454
S R + G + ++M R + S+ R + + + L +++LP
Sbjct: 510 KNSARGRGAARGRGVQ----TNMMR----GRGSAKSVERRDANTWVHLIGHLHKQNLLPV 569
Query: 455 VWFIFSRKGCDAAVQYIEGSNLLDDCERSEVELALRK--FRIQFPDALRESAVK---GLL 514
+ F+FS+K C+ V + +L + E+SEV + + K R++ D L + L
Sbjct: 570 IVFVFSKKRCEEYVDTLTNRDLNNHQEKSEVHVVIEKAVARLKKEDRLLPQIGRMREMLS 629
Query: 515 QGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVIASLSKRSNNGR 574
+G+A HH G LP+ K +E LFQRGLVKV+FATET A G+NMPA++ V + K
Sbjct: 630 RGLAVHHGGLLPIIKEIVEILFQRGLVKVLFATETFAMGVNMPAKSVVFSGTQKHDGRNF 689
Query: 575 TQLSSNELLQMAGRAGRRGIDKKGHVVLL-QTPYEGAEECCKLLFAGIEPLVSQFTASYG 634
L E Q +GRAGRRG+D G V++L ++ ++ L+SQF +Y
Sbjct: 690 RDLLPGEYTQCSGRAGRRGLDVTGTVIILSRSELPDTASLRHMIMGPSSKLISQFRLTYN 749
Query: 635 MVLNLLAGAKVTHTSEMDETKAFQAGRTLEEARKLVEQSFGNYVGSNVMLAAKEELVKIE 688
M+LNLL T +++ ++++SF V ++ +E++ E
Sbjct: 750 MILNLLR----VETLRIED---------------MIKRSFSENVNQTLVPQHEEKIKSFE 804
BLAST of HG10022309 vs. ExPASy TrEMBL
Match:
A0A1S3CQZ5 (LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503332 PE=4 SV=1)
HSP 1 Score: 2047.3 bits (5303), Expect = 0.0e+00
Identity = 1081/1176 (91.92%), Postives = 1102/1176 (93.71%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLW-PPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPF 60
MA PAI+VYSI S Q L PPLSYPFPSF CP YSSP IQASRF +P L Y TYP
Sbjct: 1 MAIPAIDVYSIISLQHLCPPPLSYPFPSFVCPHYSSPSIQASRFCRHKP--LAFYSTYPV 60
Query: 61 RFRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVEL 120
RFRPSFQSPRSIFS+KSQLSDVDEDEDEDEDEDD+DDVAAEEYDSDALG FEQSYDEVEL
Sbjct: 61 RFRPSFQSPRSIFSEKSQLSDVDEDEDEDEDEDDEDDVAAEEYDSDALGEFEQSYDEVEL 120
Query: 121 SMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
SM+A+EIS APEEFKWQRVEKLLGEV+EFG+GIIDVDELASVYNFRIDKFQRLAIQAFLR
Sbjct: 121 SMDASEISNAPEEFKWQRVEKLLGEVREFGEGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
Query: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL
Sbjct: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
Query: 241 LTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTV 300
LTGDSAVNKDA VLIMTTEILRNMLYQSVGMA+SASGLFHVDVIVLDEVHYLSDISRGTV
Sbjct: 241 LTGDSAVNKDAPVLIMTTEILRNMLYQSVGMATSASGLFHVDVIVLDEVHYLSDISRGTV 300
Query: 301 WEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSL 360
WEEI IHGKTELVTSSKRPVPLTWHFSTKTSL
Sbjct: 301 WEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSSKRPVPLTWHFSTKTSL 360
Query: 361 LPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQA 420
LPLLDEKG RMN RKLSLNYLQLHASGAKLYKDDGS RR PKR GNEISYDN+ SMSRQA
Sbjct: 361 LPLLDEKGTRMN-RKLSLNYLQLHASGAKLYKDDGS-RRTPKRRGNEISYDNIGSMSRQA 420
Query: 421 TLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCE 480
TLSKNDINSIRRSNVPQVIDTLWQLK+KDMLPAVWFIFSRKGCDAAVQY++ SNLLDDCE
Sbjct: 421 TLSKNDINSIRRSNVPQVIDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYVDSSNLLDDCE 480
Query: 481 RSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
RSEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA
Sbjct: 481 RSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
Query: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTP 600
TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGID KGHVVLLQTP
Sbjct: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDXKGHVVLLQTP 600
Query: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEAR 660
YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETK FQA RTLEEAR
Sbjct: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKTFQAWRTLEEAR 660
Query: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE
Sbjct: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
Query: 721 LQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVL 780
LQEELRSEKRHRTELRKEMESQRI ALNSLLRNLGDG LPFLCLQYKDSEGVQHSIP VL
Sbjct: 721 LQEELRSEKRHRTELRKEMESQRIFALNSLLRNLGDGHLPFLCLQYKDSEGVQHSIPTVL 780
Query: 781 LGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
LGNMDSSKL NMFPADNSLSG ESNL I LEPG+ESSYYVALGSDNSWYLFTEKWIKTVY
Sbjct: 781 LGNMDSSKLGNMFPADNSLSGAESNLDITLEPGSESSYYVALGSDNSWYLFTEKWIKTVY 840
Query: 841 KTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
KTGFPNVALTKGDALPREIMR LLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV
Sbjct: 841 KTGFPNVALTKGDALPREIMRSLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
Query: 901 LNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDK 960
LNSLSENDELLQMSQSYMESLERYK QRNKVARLKKRI+RTEGFREYKKILDMANL+EDK
Sbjct: 901 LNSLSENDELLQMSQSYMESLERYKVQRNKVARLKKRISRTEGFREYKKILDMANLVEDK 960
Query: 961 IRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
IRQLK RYKRLSNRI+QIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN
Sbjct: 961 IRQLKTRYKRLSNRIEQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
Query: 1021 ELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEE 1080
ELWIAMVLRNKFLVQLKPTELA VCASLVSEGIKVRPGRNNSYIFE SRTVINMINFLEE
Sbjct: 1021 ELWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKVRPGRNNSYIFEPSRTVINMINFLEE 1080
Query: 1081 QRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
QRNSL DLQEKHGVNI CCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID
Sbjct: 1081 QRNSLEDLQEKHGVNISCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
Query: 1141 LLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LLAQIPKLPD+DPSLQSNAS+AS+VMNRPPISELAG
Sbjct: 1141 LLAQIPKLPDVDPSLQSNASTASDVMNRPPISELAG 1172
BLAST of HG10022309 vs. ExPASy TrEMBL
Match:
A0A6J1D853 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018507 PE=4 SV=1)
HSP 1 Score: 2021.9 bits (5237), Expect = 0.0e+00
Identity = 1051/1175 (89.45%), Postives = 1102/1175 (93.79%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAIN YSI +P+ LWPPLSYPFP+F CP S+PPIQ SRF L+P LTI YP R
Sbjct: 1 MASPAINAYSI-APKHLWPPLSYPFPTFICPHCSTPPIQTSRFCRLKP--LTICSPYPVR 60
Query: 61 FRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVELS 120
FRPSF SPRSIFS+KSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEV+LS
Sbjct: 61 FRPSFHSPRSIFSEKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVDLS 120
Query: 121 MEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLRG 180
+E TEIST PE+FKWQRVEKLL EV+EFG+ I+DVDELAS+YNFRIDKFQRLA+QAFLRG
Sbjct: 121 IETTEISTPPEDFKWQRVEKLLSEVREFGEEIVDVDELASIYNFRIDKFQRLAVQAFLRG 180
Query: 181 SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGLL 240
SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFG++NVGLL
Sbjct: 181 SSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGENNVGLL 240
Query: 241 TGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW 300
TGDSAVNKDA +LIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW
Sbjct: 241 TGDSAVNKDAQILIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVW 300
Query: 301 EEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSLL 360
EEI IHG+TELVTSSKRPVPLTWHFSTKTSLL
Sbjct: 301 EEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGQTELVTSSKRPVPLTWHFSTKTSLL 360
Query: 361 PLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQAT 420
PLLDEKG RMN RKLSLNYLQLHASGAKLYKDDGSRRR PKRHGNEISYD+ SSMSRQAT
Sbjct: 361 PLLDEKGTRMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRHGNEISYDSTSSMSRQAT 420
Query: 421 LSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCER 480
LSKNDIN+IRRSNVPQV+DTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEG NLLD+CER
Sbjct: 421 LSKNDINTIRRSNVPQVMDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGRNLLDECER 480
Query: 481 SEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT 540
SEVELALR+FRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT
Sbjct: 481 SEVELALRRFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFAT 540
Query: 541 ETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPY 600
ETLAAGINMPAR+AVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGID+KGHVVL+QTPY
Sbjct: 541 ETLAAGINMPARSAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDQKGHVVLIQTPY 600
Query: 601 EGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEARK 660
EGAEECCK+LFAGIEPLVSQFTASYGMVLNLLAGAKVTH+SE+DETKAF AGRTLEEARK
Sbjct: 601 EGAEECCKILFAGIEPLVSQFTASYGMVLNLLAGAKVTHSSEIDETKAFLAGRTLEEARK 660
Query: 661 LVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAEL 720
LVEQSFGNYVGSNVMLAAKEELVKIEKEI+MLNLEIT+EAIDRKSRKFLS+VAYNEIAEL
Sbjct: 661 LVEQSFGNYVGSNVMLAAKEELVKIEKEIKMLNLEITEEAIDRKSRKFLSEVAYNEIAEL 720
Query: 721 QEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVLL 780
QEELRSEKRHRTELRKEME QRI AL SLL+NLGDG LPFLCLQYKDSEGVQHS+PAVLL
Sbjct: 721 QEELRSEKRHRTELRKEMELQRIYALKSLLQNLGDGHLPFLCLQYKDSEGVQHSVPAVLL 780
Query: 781 GNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVYK 840
GNMDSSK NMFP DN+LS ESNLGINL+PGAESSYYVALGSDNSWYLFTEKWI+TVYK
Sbjct: 781 GNMDSSKFNNMFPVDNALSAAESNLGINLDPGAESSYYVALGSDNSWYLFTEKWIRTVYK 840
Query: 841 TGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPVL 900
TGFPNVALTKGDALPRE+MR LLDKEGM WEKLADSELGSL+CMEGSLETWSWSLNVPVL
Sbjct: 841 TGFPNVALTKGDALPRELMRTLLDKEGMXWEKLADSELGSLSCMEGSLETWSWSLNVPVL 900
Query: 901 NSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDKI 960
NSLSENDELLQMS SYMESLERYKEQRN+VARLKKRIARTEGFREYKK+LDMAN+IEDKI
Sbjct: 901 NSLSENDELLQMSSSYMESLERYKEQRNRVARLKKRIARTEGFREYKKVLDMANIIEDKI 960
Query: 961 RQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE 1020
RQLKAR KRLSNRI+QIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE
Sbjct: 961 RQLKARSKRLSNRIEQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENE 1020
Query: 1021 LWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEEQ 1080
LWIAMVLRNKFLVQLKPTELA VCASLVSEGIK+RPGRNNSYIFE S TV+NMI+FLEEQ
Sbjct: 1021 LWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKIRPGRNNSYIFEPSTTVMNMIDFLEEQ 1080
Query: 1081 RNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL 1140
R+SLL LQEKHGV+IPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL
Sbjct: 1081 RSSLLHLQEKHGVDIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDL 1140
Query: 1141 LAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LAQIPKLPDIDPSLQSNAS+ASNVMNRPPISELAG
Sbjct: 1141 LAQIPKLPDIDPSLQSNASTASNVMNRPPISELAG 1171
BLAST of HG10022309 vs. ExPASy TrEMBL
Match:
A0A0A0KNK4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G484640 PE=4 SV=1)
HSP 1 Score: 2016.1 bits (5222), Expect = 0.0e+00
Identity = 1067/1176 (90.73%), Postives = 1092/1176 (92.86%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLW-PPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPF 60
MA PAINVYSI S Q L PPLSYPFPSF P YS ASRF +P L Y T P
Sbjct: 1 MAIPAINVYSIISLQHLCPPPLSYPFPSFLTPHYS-----ASRFCPHKP--LAFYSTRPV 60
Query: 61 RFRPSFQSPRSIFSQKSQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVEL 120
FRPSF SPRSIFS+KSQLSDVDEDEDEDEDEDD+DDVAAEEYDSDALGGFEQSYDEVEL
Sbjct: 61 PFRPSFHSPRSIFSEKSQLSDVDEDEDEDEDEDDEDDVAAEEYDSDALGGFEQSYDEVEL 120
Query: 121 SMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLR 180
SM+ +EIS A +EFKWQRVEKLLGEV+EFG+GIIDVDELASVYNFRIDKFQRLA+QAFLR
Sbjct: 121 SMDTSEISNASQEFKWQRVEKLLGEVREFGEGIIDVDELASVYNFRIDKFQRLAVQAFLR 180
Query: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL
Sbjct: 181 GSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGL 240
Query: 241 LTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTV 300
LTGDSAVNKDA VLIMTTEILRNMLYQSVGMA+SASGLFHVDVIVLDEVHYLSDISRGTV
Sbjct: 241 LTGDSAVNKDAPVLIMTTEILRNMLYQSVGMATSASGLFHVDVIVLDEVHYLSDISRGTV 300
Query: 301 WEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTKTSL 360
WEEI IHGKTELVTSSKRPVPLTWHFSTKTSL
Sbjct: 301 WEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSSKRPVPLTWHFSTKTSL 360
Query: 361 LPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQA 420
LPLLDEKGARMN RKLSLNYLQL+ASG K KDDGSRRR PKR GNEISYDN+ SMSRQA
Sbjct: 361 LPLLDEKGARMN-RKLSLNYLQLNASGDKFSKDDGSRRRTPKRRGNEISYDNIGSMSRQA 420
Query: 421 TLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCE 480
TLSKNDINSIRRSNVPQVIDTLWQLK+KDMLPAVWFIFSRKGCDAAVQYI+ SNLLDDCE
Sbjct: 421 TLSKNDINSIRRSNVPQVIDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIDSSNLLDDCE 480
Query: 481 RSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
RSEVELALRKFRIQFPDA+RESA+KGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA
Sbjct: 481 RSEVELALRKFRIQFPDAVRESAIKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFA 540
Query: 541 TETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTP 600
TETLAAGINMPARTAVIASLSKRSNNGRT LS NELLQMAGRAGRRGIDKKGHVVLLQTP
Sbjct: 541 TETLAAGINMPARTAVIASLSKRSNNGRTHLSPNELLQMAGRAGRRGIDKKGHVVLLQTP 600
Query: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLEEAR 660
YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQA RTLEEAR
Sbjct: 601 YEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAWRTLEEAR 660
Query: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAE 720
KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSD+AYNEIAE
Sbjct: 661 KLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDMAYNEIAE 720
Query: 721 LQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVL 780
LQEELR EKRHRTELRKEMESQRICALNSLLRNLGDG LPFLCLQYKDSEGVQHSIP VL
Sbjct: 721 LQEELRLEKRHRTELRKEMESQRICALNSLLRNLGDGHLPFLCLQYKDSEGVQHSIPTVL 780
Query: 781 LGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
LGNMDSSKL NMFPAD+SLSG ESNLGI LEPGAESSYYVALGSDNSWYLFTEKWIKTVY
Sbjct: 781 LGNMDSSKLGNMFPADSSLSGAESNLGITLEPGAESSYYVALGSDNSWYLFTEKWIKTVY 840
Query: 841 KTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
KTGFPNVAL+KGDALPREIMR LLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV
Sbjct: 841 KTGFPNVALSKGDALPREIMRSLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPV 900
Query: 901 LNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDK 960
LNSLSENDELLQMSQSYMESL+RYK QRNKVARLKKRI++TEGFREYKKILDMANLIEDK
Sbjct: 901 LNSLSENDELLQMSQSYMESLDRYKVQRNKVARLKKRISKTEGFREYKKILDMANLIEDK 960
Query: 961 IRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
IRQLK RYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN
Sbjct: 961 IRQLKTRYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGEN 1020
Query: 1021 ELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEE 1080
ELWIAMVLRNKFLVQLKPTELA VCASLVSEGIKVRPGRNNSYIFE SRTVINMINFLEE
Sbjct: 1021 ELWIAMVLRNKFLVQLKPTELAAVCASLVSEGIKVRPGRNNSYIFEPSRTVINMINFLEE 1080
Query: 1081 QRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTID 1140
QRNSL DLQEKHGVNI CCLDSQFSGMVEAWASGLTWRE+MMDCAMDEGDLARLLRRTID
Sbjct: 1081 QRNSLEDLQEKHGVNISCCLDSQFSGMVEAWASGLTWREIMMDCAMDEGDLARLLRRTID 1140
Query: 1141 LLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
LLAQIPKLPDIDPSLQ NAS+AS+VMNRPPISELAG
Sbjct: 1141 LLAQIPKLPDIDPSLQRNASTASDVMNRPPISELAG 1168
BLAST of HG10022309 vs. ExPASy TrEMBL
Match:
A0A6J1G401 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111450542 PE=4 SV=1)
HSP 1 Score: 1993.4 bits (5163), Expect = 0.0e+00
Identity = 1046/1183 (88.42%), Postives = 1088/1183 (91.97%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAINV+SI SPQ LWPPLSYPFPS C + SSPPIQA RF L+P L I+ +P
Sbjct: 1 MASPAINVFSIISPQHLWPPLSYPFPSSLCSRCSSPPIQAPRFCRLKP--LPIFYPFPVP 60
Query: 61 FRPSFQSPRSIFSQKSQLSDV--------DEDEDEDEDEDDDDDVAAEEYDSDALGGFEQ 120
FRPS +SPRSIFS+K QLSDV DEDEDEDEDEDDDDDVAAEEYD DA G EQ
Sbjct: 61 FRPSVRSPRSIFSEKPQLSDVDEDEDEDEDEDEDEDEDEDDDDDVAAEEYDIDAFGELEQ 120
Query: 121 SYDEVELSMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRL 180
YDEVELSMEATEIS APEEFK QRVEKLLGEV+EFG+GI+DVDELASVYNFRIDKFQR
Sbjct: 121 RYDEVELSMEATEISIAPEEFKSQRVEKLLGEVREFGEGIVDVDELASVYNFRIDKFQRQ 180
Query: 181 AIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF 240
AI+AFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF
Sbjct: 181 AIEAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETF 240
Query: 241 GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLS 300
GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASS SGLFHVDVIVLDEVHYLS
Sbjct: 241 GDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSESGLFHVDVIVLDEVHYLS 300
Query: 301 DISRGTVWEEI-----------------------------IHGKTELVTSSKRPVPLTWH 360
DISRGTVWEEI IHGKTELVTS+KRPVPLTWH
Sbjct: 301 DISRGTVWEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSTKRPVPLTWH 360
Query: 361 FSTKTSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNV 420
FSTKTSLLPLLDEKG MN RKLSLNYLQLHASGAKLYKDDGSRRR PKRHGNEIS D
Sbjct: 361 FSTKTSLLPLLDEKGKHMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRHGNEISNDRT 420
Query: 421 SSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGS 480
SS+SRQATLSKNDINSIRRSNVPQV+DTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEG
Sbjct: 421 SSLSRQATLSKNDINSIRRSNVPQVVDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGK 480
Query: 481 NLLDDCERSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG 540
NLLDDCERSEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG
Sbjct: 481 NLLDDCERSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRG 540
Query: 541 LVKVVFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGH 600
LVKVVFATETLAAGINMPARTAVIASLSKRSN+GRTQL+SNELLQMAGRAGRRGIDKKGH
Sbjct: 541 LVKVVFATETLAAGINMPARTAVIASLSKRSNSGRTQLTSNELLQMAGRAGRRGIDKKGH 600
Query: 601 VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAG 660
VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMD TKAFQAG
Sbjct: 601 VVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDGTKAFQAG 660
Query: 661 RTLEEARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDV 720
RTLEEARKLVEQSFGNYVGSNVM+AAKEELVKIEKEIE+LNLEITDEAID+K R+ +SDV
Sbjct: 661 RTLEEARKLVEQSFGNYVGSNVMIAAKEELVKIEKEIEVLNLEITDEAIDKKCRRLMSDV 720
Query: 721 AYNEIAELQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQ 780
AYNE+ ELQE LRS+KR RTELRKEME QRICA SLL+NLGDGQLPFLCLQYKDSEGVQ
Sbjct: 721 AYNELVELQEGLRSDKRQRTELRKEMELQRICAFKSLLQNLGDGQLPFLCLQYKDSEGVQ 780
Query: 781 HSIPAVLLGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTE 840
HSIPAVL GNMDSSKLINMFPADNSLSG E N G NL+PGAESSYYVALGSDNSWYLFTE
Sbjct: 781 HSIPAVLFGNMDSSKLINMFPADNSLSGAEPNFGKNLDPGAESSYYVALGSDNSWYLFTE 840
Query: 841 KWIKTVYKTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWS 900
KWIKTVYKTGFPNVALTKGD LPREIMR LLDKEGMKWEKLADSELGSL+CMEGSLETWS
Sbjct: 841 KWIKTVYKTGFPNVALTKGDPLPREIMRTLLDKEGMKWEKLADSELGSLSCMEGSLETWS 900
Query: 901 WSLNVPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDM 960
WSLNVPVLNSLSENDELLQMS+SYMESL RYKEQRNKV+R KKRI+RT+GFREYKKILDM
Sbjct: 901 WSLNVPVLNSLSENDELLQMSESYMESLTRYKEQRNKVSRFKKRISRTKGFREYKKILDM 960
Query: 961 ANLIEDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETA 1020
AN+IEDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDIN+HVMFPLGETA
Sbjct: 961 ANIIEDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINSHVMFPLGETA 1020
Query: 1021 AAIRGENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVIN 1080
AAIRGENELWIA+VLRNKFLVQLKPTELAGVCASLVSEGIK+RPGR+N+YIFE SRTVIN
Sbjct: 1021 AAIRGENELWIAVVLRNKFLVQLKPTELAGVCASLVSEGIKIRPGRDNNYIFEPSRTVIN 1080
Query: 1081 MINFLEEQRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLAR 1140
M+NFLEEQRNSLLDLQEKHGVNIPC LDSQFSGMVEAWASGLTWRE+MMDCAMDEGDLAR
Sbjct: 1081 MVNFLEEQRNSLLDLQEKHGVNIPCSLDSQFSGMVEAWASGLTWREIMMDCAMDEGDLAR 1140
Query: 1141 LLRRTIDLLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
L RRTIDLLAQIPKLPDIDPSLQ NAS+AS+VMNR PISELAG
Sbjct: 1141 LFRRTIDLLAQIPKLPDIDPSLQRNASTASDVMNRSPISELAG 1180
BLAST of HG10022309 vs. ExPASy TrEMBL
Match:
A0A6J1KF30 (DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111493731 PE=4 SV=1)
HSP 1 Score: 1992.2 bits (5160), Expect = 0.0e+00
Identity = 1046/1179 (88.72%), Postives = 1088/1179 (92.28%), Query Frame = 0
Query: 1 MASPAINVYSISSPQRLWPPLSYPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFR 60
MASPAINV+SI SPQ LWPPLSYPFPS CP+ SPPIQA RF L+P L I+ +P
Sbjct: 1 MASPAINVFSIISPQHLWPPLSYPFPSSLCPRCFSPPIQAPRFCRLKP--LPIFSPFPVP 60
Query: 61 FRPSFQSPRSIFSQKSQLSDV----DEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDE 120
FRPS +SPRSIFS+K QLSDV DEDEDEDEDEDDDDDVAAEEYD DA G EQ YDE
Sbjct: 61 FRPSVRSPRSIFSEKPQLSDVDEDEDEDEDEDEDEDDDDDVAAEEYDIDAFGELEQRYDE 120
Query: 121 VELSMEATEISTAPEEFKWQRVEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQA 180
VELSMEATEIS APEEFK RVEKLLGEV+EFG+GI+DVDELASVYNFRIDKFQR AI+A
Sbjct: 121 VELSMEATEISIAPEEFKSHRVEKLLGEVREFGEGIVDVDELASVYNFRIDKFQRQAIEA 180
Query: 181 FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN 240
FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN
Sbjct: 181 FLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSN 240
Query: 241 VGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISR 300
VGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASS SGLFHVDVIVLDEVHYLSDISR
Sbjct: 241 VGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSESGLFHVDVIVLDEVHYLSDISR 300
Query: 301 GTVWEEI-----------------------------IHGKTELVTSSKRPVPLTWHFSTK 360
GTVWEEI IHGKTELVTS+KRPVPLTWHFSTK
Sbjct: 301 GTVWEEIVIYCPKEVQLICLSATVANPDELAGWIGQIHGKTELVTSTKRPVPLTWHFSTK 360
Query: 361 TSLLPLLDEKGARMNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMS 420
SLLPLLDEKG MN RKLSLNYLQLHASGAKLYKDDGSRRR PKRHGNEIS D SS+S
Sbjct: 361 ASLLPLLDEKGTHMN-RKLSLNYLQLHASGAKLYKDDGSRRRNPKRHGNEISNDRTSSLS 420
Query: 421 RQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLD 480
RQATLSKNDINSIRRSNVPQV+DTLWQLK+KDMLPAVWFIFSRKGCDAAVQYIEG NLLD
Sbjct: 421 RQATLSKNDINSIRRSNVPQVVDTLWQLKSKDMLPAVWFIFSRKGCDAAVQYIEGKNLLD 480
Query: 481 DCERSEVELALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV 540
DCE SEVELALRKFRIQFPDA+RESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV
Sbjct: 481 DCESSEVELALRKFRIQFPDAVRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV 540
Query: 541 VFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLL 600
VFATETLAAGINMPARTAVIASLSKRSN+GRTQL+SNELLQMAGRAGRRGIDKKGHVVLL
Sbjct: 541 VFATETLAAGINMPARTAVIASLSKRSNSGRTQLTSNELLQMAGRAGRRGIDKKGHVVLL 600
Query: 601 QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDETKAFQAGRTLE 660
QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMD TKAFQAGRTLE
Sbjct: 601 QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSEMDGTKAFQAGRTLE 660
Query: 661 EARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNE 720
EARKLVEQSFGNYVGSNVM+AAKEELVKIEKEIE+LNLEITDEAI++K R+ LSDVAYNE
Sbjct: 661 EARKLVEQSFGNYVGSNVMIAAKEELVKIEKEIEVLNLEITDEAIEKKCRRLLSDVAYNE 720
Query: 721 IAELQEELRSEKRHRTELRKEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIP 780
+ ELQEELRS+KR RTELRKEME QRI AL SLL+NLGDGQLPFLCLQYKDSEGVQHSIP
Sbjct: 721 LVELQEELRSDKRQRTELRKEMELQRIFALKSLLQNLGDGQLPFLCLQYKDSEGVQHSIP 780
Query: 781 AVLLGNMDSSKLINMFPADNSLSGTESNLGINLEPGAESSYYVALGSDNSWYLFTEKWIK 840
AVL GNMDSSKLINMFPADNSLSG E NLG NL+PGAESSYYVALGSDNSWYLFTEKWIK
Sbjct: 781 AVLFGNMDSSKLINMFPADNSLSGAEPNLGKNLDPGAESSYYVALGSDNSWYLFTEKWIK 840
Query: 841 TVYKTGFPNVALTKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLN 900
TVYKTGFPNVALTKGD LPREIMR LLDKEGMKWEKLADSELGSL+CMEGSLETWSWSLN
Sbjct: 841 TVYKTGFPNVALTKGDPLPREIMRTLLDKEGMKWEKLADSELGSLSCMEGSLETWSWSLN 900
Query: 901 VPVLNSLSENDELLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLI 960
VPVLNSLSENDELLQMS+SYMESL RYKEQRNKV+R KKRI+RTEGFREYKKILDMAN+I
Sbjct: 901 VPVLNSLSENDELLQMSESYMESLTRYKEQRNKVSRFKKRISRTEGFREYKKILDMANII 960
Query: 961 EDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIR 1020
EDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDIN+HVMFPLGETAAAIR
Sbjct: 961 EDKIRQLKARYKRLSNRIQQIEPSGWKEFLQISNVIHEIRALDINSHVMFPLGETAAAIR 1020
Query: 1021 GENELWIAMVLRNKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINF 1080
GENELWIA+VLRNKFLVQLKPTELAGVCASLVSEGIK+RPGR+N+YIFE SRTVINM+NF
Sbjct: 1021 GENELWIAVVLRNKFLVQLKPTELAGVCASLVSEGIKIRPGRDNNYIFEPSRTVINMVNF 1080
Query: 1081 LEEQRNSLLDLQEKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRR 1140
LEEQRNSLLDLQEKHGV+IPC LDSQFSGMVEAWASGLTWRE+MMDCAMDEGDLARLLRR
Sbjct: 1081 LEEQRNSLLDLQEKHGVDIPCSLDSQFSGMVEAWASGLTWREIMMDCAMDEGDLARLLRR 1140
Query: 1141 TIDLLAQIPKLPDIDPSLQSNASSASNVMNRPPISELAG 1147
TIDLLAQIPKLPDIDPSLQ NAS+AS+VMNR PISELAG
Sbjct: 1141 TIDLLAQIPKLPDIDPSLQRNASTASDVMNRSPISELAG 1176
BLAST of HG10022309 vs. TAIR 10
Match:
AT1G70070.1 (DEAD/DEAH box helicase, putative )
HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 776/1167 (66.50%), Postives = 939/1167 (80.46%), Query Frame = 0
Query: 23 YPFPSFKCPQYSSPPIQASRFFSLQPLGLTIYCTYPFRFRPSFQSPRSIFSQKSQLSDVD 82
+ FPS SP ++ L P L+ T S+ +SQL + +
Sbjct: 18 FHFPSLHRSLSHSPNFSFTKSLILNPNHLSFKSTL-----------NSLSPSQSQLYEEE 77
Query: 83 EDEDEDEDEDDDDDVAAEEYD--SDALGGFEQSYD--EVELSME-ATEISTAPEEFKWQR 142
+DE+E+E+++DDDD AA+EYD SD + + D E E S++ TE + EF+WQR
Sbjct: 78 DDEEEEEEDEDDDDEAADEYDNISDEIRNSDDDDDDEETEFSVDLPTESARERVEFRWQR 137
Query: 143 VEKLLGEVKEFGDGIIDVDELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIA 202
VEKL V++FG +ID+DEL S+Y+FRIDKFQRLAI+AFLRGSSVVVSAPTSSGKTLIA
Sbjct: 138 VEKLRSLVRDFGVEMIDIDELISIYDFRIDKFQRLAIEAFLRGSSVVVSAPTSSGKTLIA 197
Query: 203 EAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTT 262
EAAAV+TVA+ RRLFYTTPLKALSNQKFREFRETFGD NVGLLTGDSA+NKDA ++IMTT
Sbjct: 198 EAAAVSTVAKGRRLFYTTPLKALSNQKFREFRETFGDDNVGLLTGDSAINKDAQIVIMTT 257
Query: 263 EILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVWEEI-------------- 322
EILRNMLYQSVGMASS +GLFHVD IVLDEVHYLSDISRGTVWEEI
Sbjct: 258 EILRNMLYQSVGMASSGTGLFHVDAIVLDEVHYLSDISRGTVWEEIVIYCPKEVQLICLS 317
Query: 323 ---------------IHGKTELVTSSKRPVPLTWHFSTKTSLLPLLDEKGARMNGRKLSL 382
IHGKTELVTS++RPVPLTW+FSTK SLLPLLDEKG +N RKLSL
Sbjct: 318 ATVANPDELAGWIGEIHGKTELVTSTRRPVPLTWYFSTKHSLLPLLDEKGINVN-RKLSL 377
Query: 383 NYLQLHASGAKLY-KDDGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSIRRSNVPQ 442
NYLQL AS A+ DDG R+R+ K+ G + SY+N+ +++ LSKN+IN IRRS VPQ
Sbjct: 378 NYLQLSASEARFRDDDDGYRKRRSKKRGGDTSYNNLVNVT-DYPLSKNEINKIRRSQVPQ 437
Query: 443 VIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCERSEVELALRKFRIQFPD 502
+ DTLW L+ K+MLPA+WFIF+R+GCDAAVQY+E LLDDCE+SEVELAL+KFR+ +PD
Sbjct: 438 ISDTLWHLQGKNMLPAIWFIFNRRGCDAAVQYVENFQLLDDCEKSEVELALKKFRVLYPD 497
Query: 503 ALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI 562
A+RESA KGLL+G+AAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI
Sbjct: 498 AVRESAEKGLLRGIAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAGINMPARTAVI 557
Query: 563 ASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTPYEGAEECCKLLFAGIEP 622
+SLSK++ N R +L NEL QMAGRAGRRGID+KG+ VL+QT +EGAEECCKL+FAG++P
Sbjct: 558 SSLSKKAGNERIELGPNELYQMAGRAGRRGIDEKGYTVLVQTAFEGAEECCKLVFAGVKP 617
Query: 623 LVSQFTASYGMVLNLLAGAKVTHTSEMDET-KAFQAGRTLEEARKLVEQSFGNYVGSNVM 682
LVSQFTASYGMVLNL+AG+KVT S E K QAGR+LEEA+KLVE+SFGNYV SNV
Sbjct: 618 LVSQFTASYGMVLNLVAGSKVTRKSSGTEAGKVLQAGRSLEEAKKLVEKSFGNYVSSNVT 677
Query: 683 LAAKEELVKIEKEIEMLNLEITDEAIDRKSRKFLSDVAYNEIAELQEELRSEKRHRTELR 742
+AAK+EL +I+ +IE+L+ EI+DEAID+KSRK LS Y EI L+EELR EKR R E R
Sbjct: 678 VAAKQELAEIDNKIEILSSEISDEAIDKKSRKLLSARDYKEITVLKEELREEKRKRAEQR 737
Query: 743 KEMESQRICALNSLLRNLGDGQLPFLCLQYKDSEGVQHSIPAVLLGNMDS---SKLINMF 802
+ ME +R AL LL+ + +G LPF+CL++KDSEG + S+PAV LG++DS SKL M
Sbjct: 738 RRMELERFLALKPLLKGMEEGNLPFICLEFKDSEGREQSVPAVYLGHIDSFQGSKLQKMM 797
Query: 803 PADNS--LSGTESNLGINL--EPGAESSYYVALGSDNSWYLFTEKWIKTVYKTGFPNVAL 862
D S L+ E L + +P + SYYVALGSDNSWYLFTEKW++TVY+TGFPN+AL
Sbjct: 798 SLDESFALNLIEDELAADEPGKPNVKPSYYVALGSDNSWYLFTEKWVRTVYRTGFPNIAL 857
Query: 863 TKGDALPREIMRDLLDKEGMKWEKLADSELGSLACMEGSLETWSWSLNVPVLNSLSENDE 922
GDALPREIM++LLDK M+W+KLA+SELGSL +EGSLETWSWSLNVPVL+SLS+ DE
Sbjct: 858 ALGDALPREIMKNLLDKADMQWDKLAESELGSLWRLEGSLETWSWSLNVPVLSSLSDEDE 917
Query: 923 LLQMSQSYMESLERYKEQRNKVARLKKRIARTEGFREYKKILDMANLIEDKIRQLKARYK 982
+L MS+ Y + ++YKEQR+K++RLKK+++R+EGFREYKKIL+ ANL +K+++LKAR +
Sbjct: 918 VLHMSEEYDNAAQKYKEQRSKISRLKKKMSRSEGFREYKKILENANLTVEKMKRLKARSR 977
Query: 983 RLSNRIQQIEPSGWKEFLQISNVIHEIRALDINTHVMFPLGETAAAIRGENELWIAMVLR 1042
RL NR++QIEPSGWK+F++ISNVIHE RALDINTH++FPLGETAAAIRGENELW+AMVLR
Sbjct: 978 RLINRLEQIEPSGWKDFMRISNVIHESRALDINTHLIFPLGETAAAIRGENELWLAMVLR 1037
Query: 1043 NKFLVQLKPTELAGVCASLVSEGIKVRPGRNNSYIFEASRTVINMINFLEEQRNSLLDLQ 1102
NK LV LKP +LAGVCASLVSEGIKVRP R+N+YI+E S TV++M+NFLE+QR+SL+ LQ
Sbjct: 1038 NKALVDLKPPQLAGVCASLVSEGIKVRPWRDNNYIYEPSDTVVDMVNFLEDQRSSLIKLQ 1097
Query: 1103 EKHGVNIPCCLDSQFSGMVEAWASGLTWREMMMDCAMDEGDLARLLRRTIDLLAQIPKLP 1147
EKH V IPCCLD QFSGMVEAWASGL+W+EMMM+CAMDEGDLARLLRRTIDLLAQIPKLP
Sbjct: 1098 EKHEVMIPCCLDVQFSGMVEAWASGLSWKEMMMECAMDEGDLARLLRRTIDLLAQIPKLP 1157
BLAST of HG10022309 vs. TAIR 10
Match:
AT3G46960.1 (RNA helicase, ATP-dependent, SK12/DOB1 protein )
HSP 1 Score: 218.4 bits (555), Expect = 3.0e-56
Identity = 189/626 (30.19%), Postives = 297/626 (47.44%), Query Frame = 0
Query: 76 SQLSDVDEDEDEDEDEDDDDDVAAEEYDSDALGGFEQSYDEVELSMEATEISTAPEEFKW 135
S+L D + + + + A ++ G E ++ + + + + EE
Sbjct: 262 SELEGDDHTAGSESPKAEAEPDAKASISNEVSKGLETDVTVLDEILSSAKTAIMSEEAVT 321
Query: 136 QRVEKLL--------GEVKEFGDGIID-VDELASVYNFRIDKFQRLAIQAFLRGSSVVVS 195
+K L G+ ++ D + V ++A + F +D FQ+ AI +G SV V+
Sbjct: 322 GSSDKQLRKEGWATKGDSQDIADRFYELVPDMAIEFPFELDNFQKEAICCLEKGESVFVA 381
Query: 196 APTSSGKTLIAEAAAVATVARKRRLFYTTPLKALSNQKFREFRETFGDSNVGLLTGDSAV 255
A TS+GKT++AE A R YT P+K +SNQK+R+F G +VGLLTGD ++
Sbjct: 382 AHTSAGKTVVAEYAFALATKHCTRAVYTAPIKTISNQKYRDF---CGKFDVGLLTGDVSI 441
Query: 256 NKDALVLIMTTEILRNMLYQSVGMASSASGLFHVDVIVLDEVHYLSDISRGTVWEEII-- 315
+A LIMTTEILR+MLY+ A + ++ ++ DEVHY++D+ RG VWEE+I
Sbjct: 442 RPEASCLIMTTEILRSMLYR------GADIIRDIEWVIFDEVHYVNDVERGVVWEEVIIM 501
Query: 316 -----------------------HGKTE-----LVTSSKRPVPLTWHFSTKTSLLPLLDE 375
G+T+ + ++KRPVPL L + +
Sbjct: 502 LPRHINFVLLSATVPNTFEFADWIGRTKQKEIRVTGTTKRPVPLEHCLFYSGELYKVCEN 561
Query: 376 --------KGARMNGRKLSLNYLQL----------HASGAKLYKDDGSRRRKPKRHGNEI 435
K A+ + +K + N + + H G+K K + R K +H
Sbjct: 562 EVFLSKGIKDAKDSQKKKNSNAVSVAPKQQMGSSAHQDGSKSQKHEAHSRGKQNKHS--- 621
Query: 436 SYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQ 495
S +V S N + RRS + + +L +LP V F FS+ CD
Sbjct: 622 SVKDVGKSSYSGNSQNN--GAFRRSAASNWLLLINKLSKMSLLPVVVFCFSKNYCDRCAD 681
Query: 496 YIEGSNLLDDCERSEVEL-------ALRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPL 555
+ G++L E+SE+ + L+ P LR ++ L +G+ HHAG LP+
Sbjct: 682 ALTGTDLTSSSEKSEIRVFCDKAFSRLKGSDRNLPQVLRLQSL--LHRGIGVHHAGLLPI 741
Query: 556 WKSFIEELFQRGLVKVVFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAG 615
K +E LF RG++KV+F+TET A G+N PART V +L K QL E QMAG
Sbjct: 742 VKEVVEMLFCRGVIKVLFSTETFAMGVNAPARTVVFDALRKFDGKEFRQLLPGEYTQMAG 801
Query: 616 RAGRRGIDKKGHVVLL---QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKV 634
RAGRRG+DK G VV++ + P E + +++ L SQF +Y M+L+LL ++
Sbjct: 802 RAGRRGLDKTGTVVVMCRDEVPDE--SDLRRVIVGSATRLESQFRLTYIMILHLLRVEEL 861
BLAST of HG10022309 vs. TAIR 10
Match:
AT1G59760.1 (RNA helicase, ATP-dependent, SK12/DOB1 protein )
HSP 1 Score: 213.8 bits (543), Expect = 7.4e-55
Identity = 153/456 (33.55%), Postives = 229/456 (50.22%), Query Frame = 0
Query: 159 ASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTPLK 218
A + F +D FQ AI+ G SV+VSA TS+GKT++A A ++ +R+ YT+P+K
Sbjct: 61 AKDFPFTLDSFQSEAIKCLDNGESVMVSAHTSAGKTVVASYAIAMSLKENQRVIYTSPIK 120
Query: 219 ALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASGLF 278
ALSNQK+R+F+E F D VGL+TGD ++ +A L+MTTEILR+M Y+ + +
Sbjct: 121 ALSNQKYRDFKEEFSD--VGLMTGDVTIDPNASCLVMTTEILRSMQYK------GSEIMR 180
Query: 279 HVDVIVLDEVHYLSDISRGTVWEE--IIHGKTELVTSSKRPVPLTWHFSTKTSLLPLLDE 338
V I+ DEVHY+ D RG VWEE ++ K VP F+ + +
Sbjct: 181 EVAWIIFDEVHYMRDSERGVVWEESIVMAPKNSRFVFLSATVPNAKEFADWVAKVHQQPC 240
Query: 339 KGARMNGRKLSLNYLQLHASGAKLY--KDDGSRRRKP--KRHGNEISYDNVSSMSRQATL 398
+ R L + A G LY D+ S+ + ++ N + N S R
Sbjct: 241 HIVYTDYRPTPLQHYVFPAGGNGLYLVVDEKSKFHEDSFQKSLNALVPTNESDKKRDNGK 300
Query: 399 SKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCERS 458
+ + + + + + + P + F FS+K C+A + L D E+
Sbjct: 301 FQKGLVIGKLGEESDIFKLVKMIIQRQYDPVILFSFSKKECEALAMQMSKMVLNSDDEKD 360
Query: 459 EVELALRKFRIQFPDALRE-----SAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKV 518
VE D ++ + + L +G+ HH+G LP+ K IE LFQ GL+K
Sbjct: 361 AVETIFASAIDMLSDDDKKLPQVSNILPILKRGIGVHHSGLLPILKEVIEILFQEGLIKC 420
Query: 519 VFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLL 578
+FATET + G+NMPA+T V ++ K + LSS E +QM+GRAGRRGIDK+G +L+
Sbjct: 421 LFATETFSIGLNMPAKTVVFTNVRKFDGDKFRWLSSGEYIQMSGRAGRRGIDKRGICILM 480
Query: 579 QTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLL 604
+L + L S F SY M+LN L
Sbjct: 481 VDEKMEPAVAKSMLKGSADSLNSAFHLSYNMLLNQL 508
BLAST of HG10022309 vs. TAIR 10
Match:
AT2G06990.1 (RNA helicase, ATP-dependent, SK12/DOB1 protein )
HSP 1 Score: 212.2 bits (539), Expect = 2.1e-54
Identity = 172/566 (30.39%), Postives = 272/566 (48.06%), Query Frame = 0
Query: 157 ELASVYNFRIDKFQRLAIQAFLRGSSVVVSAPTSSGKTLIAEAAAVATVARKRRLFYTTP 216
++A Y F++D FQ +++ R S++VSA TS+GKT +AE A K+R+ YT+P
Sbjct: 73 DMAKTYPFKLDPFQSVSVACLERKESILVSAHTSAGKTAVAEYAIAMAFRDKQRVIYTSP 132
Query: 217 LKALSNQKFREFRETFGDSNVGLLTGDSAVNKDALVLIMTTEILRNMLYQSVGMASSASG 276
LKALSNQK+RE + F D VGL+TGD ++ +A L+MTTEILR MLY+ +
Sbjct: 133 LKALSNQKYRELQHEFKD--VGLMTGDVTLSPNASCLVMTTEILRAMLYR------GSEV 192
Query: 277 LFHVDVIVLDEVHYLSDISRGTVWEEIIHGKTELVTSSKRPVPLTWHFSTKTSL---LPL 336
L V ++ DE+HY+ D RG VWEE I + + + V L+ S T +
Sbjct: 193 LKEVAWVIFDEIHYMKDRERGVVWEESI----IFLPPAIKMVFLSATMSNATEFAEWICY 252
Query: 337 LDEKGARM---NGRKLSLNYLQLHASGAKLY--KDDGSRRRK----------PKRHGNEI 396
L ++ + + R L + G LY DD + R+ PK N+
Sbjct: 253 LHKQPCHVVYTDFRPTPLQHYAFPMGGGGLYLVVDDNEQFREDSFVKMQDTFPKPKSND- 312
Query: 397 SYDNVSSMSRQATLSKNDINSIRRSNVPQVIDTLWQLKAKDMLPAVWFIFSRKGCDAAVQ 456
S+ + S+V +++ + + K + P + F FSR+ C+
Sbjct: 313 --GKKSANGKSGGRGAKGGGGPGDSDVYKIVKMIMERKFE---PVIIFSFSRRECEQHAL 372
Query: 457 YIEGSNLLDDCERSEVELALRKFRIQFPDALR-----ESAVKGLLQGVAAHHAGCLPLWK 516
+ + D E+ VE + R E + L +G+A HH+G LP+ K
Sbjct: 373 SMSKLDFNTDEEKEVVEQVFNNAMQCLNEEDRSLPAIELMLPLLQRGIAVHHSGLLPVIK 432
Query: 517 SFIEELFQRGLVKVVFATETLAAGINMPARTAVIASLSKRSNNGRTQLSSNELLQMAGRA 576
+E LFQ GLVK +FATET A G+NMPA+T V ++ K + + S E +QM+GRA
Sbjct: 433 ELVELLFQEGLVKALFATETFAMGLNMPAKTVVFTAVKKWDGDSHRYIGSGEYIQMSGRA 492
Query: 577 GRRGIDKKGHVVLLQTPYEGAEECCKLLFAGIEPLVSQFTASYGMVLNLLAGAKVTHTSE 636
GRRG D++G +++ ++ PL+S F SY +LNLL+ A+ T+E
Sbjct: 493 GRRGKDERGICIIMIDEQMEMNTLRDMMLGKPAPLLSTFRLSYYTILNLLSRAEGQFTAE 552
Query: 637 MDETKAFQAGRTLEEARKLVEQSFGNYVGSNVMLAAKEELVKIEKEIEMLNLEITDEAID 696
++ SF + + ++ K+E+E +LN E +
Sbjct: 553 -----------------HVIRHSFHQFQHEKALPDIGNKVSKLEEEAAILNASGEAEVAE 601
Query: 697 RKSRKFLSDVAYNEIAELQEELRSEK 700
+ +F D+A +E + E +R E+
Sbjct: 613 YHNLQF--DIAKHEKKLMSEIIRPER 601
BLAST of HG10022309 vs. TAIR 10
Match:
AT3G27730.1 (ATP binding;ATP-dependent helicases;DNA helicases )
HSP 1 Score: 90.1 bits (222), Expect = 1.2e-17
Identity = 114/445 (25.62%), Postives = 194/445 (43.60%), Query Frame = 0
Query: 182 SVVVSAPTSSGKTLIAEAAAVATVARK-------------RRLFYTTPLKALSNQKFREF 241
++++SAPT SGKT++ E + ++ + Y +P KAL +K R++
Sbjct: 42 NMIISAPTGSGKTVLFELCILRLFSKSISKEGSFLHAKGALKTVYISPSKALVQEKLRDW 101
Query: 242 RETFGDSNVGL--LTGDSAVN-----KDALVLIMTTEILRNMLYQSVGMASSASGLF-HV 301
+ F + LTGD+ +DA +++ T E + V S G F +
Sbjct: 102 NQKFNSWGISCLELTGDNETYSTKNIQDADIILTTPEKFDAVSRYRV--TSGGLGFFSDI 161
Query: 302 DVIVLDEVHYLSDISRGTVWEEIIHGKTELVTSSKRPVPLTWHFSTKTSLLPLLDEKGAR 361
++++DEVH L+D RG E I+ + ++++S+ S+ + + LL
Sbjct: 162 ALVLIDEVHLLND-PRGAALEAIV-SRLKILSSNHE------LRSSTLASVRLLAVSATI 221
Query: 362 MNGRKLSLNYLQLHASGAKLYKDDGSRRRKPKRHGNEISYDNVSSMSRQATLSKNDINSI 421
N L+ +L++ +G KR G E+ +++ +KND
Sbjct: 222 PNIEDLA-EWLKVPTAGI-------------KRFGEEMRPVKLTTKVFGYAAAKNDFLFE 281
Query: 422 RRSNVPQVIDTLWQL-KAKDMLPAVWFIFSRKGCDAAVQYIEGSNLLDDCERSEVEL--A 481
+R + D L Q K K A+ F +RKG A Q + + + ++
Sbjct: 282 KRLQ-NYIYDILMQYSKGKS---ALVFCSTRKGAQEAAQKLAQTAMTYGYSNPFIKSREQ 341
Query: 482 LRKFRIQFPDALRESAVKGLLQGVAAHHAGCLPLWKSFIEELFQRGLVKVVFATETLAAG 541
L + R P + +LQGV H+ G +S +E LF G ++V+ T TLA G
Sbjct: 342 LERLREASPMCSDKQMQSYILQGVGYHNGGLCQKDRSLVEGLFLNGDIQVICTTNTLAHG 401
Query: 542 INMPARTAVIASLS--KRSNNGRTQLSSNELLQMAGRAGRRGIDKKGHVVLLQTP----- 595
IN+PA T VI S + + + LLQM+GRAGR D G V+++
Sbjct: 402 INLPAHTVVIKSTQHFNKEKGHYMEYDRSTLLQMSGRAGRPPFDDTGMVIIMTRRETVHL 458
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038889788.1 | 0.0e+00 | 92.96 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Benincasa hispida] | [more] |
XP_008465717.2 | 0.0e+00 | 91.92 | PREDICTED: LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH15 chlor... | [more] |
XP_022150310.1 | 0.0e+00 | 89.45 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Momordica charantia] | [more] |
XP_011655295.1 | 0.0e+00 | 90.73 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic [Cucumis sativus] >KGN5... | [more] |
KAG7029994.1 | 0.0e+00 | 88.59 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic, partial [Cucurbita arg... | [more] |
Match Name | E-value | Identity | Description | |
B9DFG3 | 0.0e+00 | 66.50 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Arabidopsis thaliana... | [more] |
Q9ZBD8 | 4.3e-68 | 36.95 | Probable helicase HelY OS=Mycobacterium leprae (strain TN) OX=272631 GN=helY PE=... | [more] |
P9WMR0 | 5.9e-65 | 33.22 | Probable helicase HelY OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)... | [more] |
P9WMR1 | 5.9e-65 | 33.22 | Probable helicase HelY OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)... | [more] |
O59801 | 1.0e-64 | 31.23 | Putative ATP-dependent RNA helicase C550.03c OS=Schizosaccharomyces pombe (strai... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CQZ5 | 0.0e+00 | 91.92 | LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS... | [more] |
A0A6J1D853 | 0.0e+00 | 89.45 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Momordica charantia ... | [more] |
A0A0A0KNK4 | 0.0e+00 | 90.73 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G484640 PE=4 SV=1 | [more] |
A0A6J1G401 | 0.0e+00 | 88.42 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Cucurbita moschata O... | [more] |
A0A6J1KF30 | 0.0e+00 | 88.72 | DExH-box ATP-dependent RNA helicase DExH15 chloroplastic OS=Cucurbita maxima OX=... | [more] |