HG10022286 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022286 vs. NCBI nr
Match: XP_022137385.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia])

HSP 1 Score: 2229.5 bits (5776), Expect = 0.0e+00
Identity = 1113/1266 (87.91%), Postives = 1168/1266 (92.26%), Query Frame = 0

Query: 2    TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 61
            T  + +S +RRR+  D N+K KKQ I++S  AEE+IESKFGF LFSEGDKRLGWLLTF  
Sbjct: 17   TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76

Query: 62   SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 121
            SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77   SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136

Query: 122  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 181
            IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196

Query: 182  DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 241
            DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197  DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256

Query: 242  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 301
            DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316

Query: 302  YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 361
            YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317  YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376

Query: 362  KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 421
            KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN
Sbjct: 377  KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 436

Query: 422  PEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTL 481
            PEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+PDEVLRKGSGTL
Sbjct: 437  PEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTPDEVLRKGSGTL 496

Query: 482  CEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFR 541
            CEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESGVFRSD+PTSFR
Sbjct: 497  CEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESGVFRSDIPTSFR 556

Query: 542  LDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------- 601
            LDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK               
Sbjct: 557  LDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRLRDGPVREECPL 616

Query: 602  --------------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRS 661
                                PPS+VTDEVCTACDFN P KTCLR+LEWVW GEVF AK+S
Sbjct: 617  IYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS 676

Query: 662  DYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEV 721
            DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPVTEV
Sbjct: 677  DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEV 736

Query: 722  RKAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLY 781
            R+AGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAK SGNSIKIQEAQDM+VLY
Sbjct: 737  REAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDMLVLY 796

Query: 782  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 841
            DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT
Sbjct: 797  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 856

Query: 842  DGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEM 901
            DGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KIY+ 
Sbjct: 857  DGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKIYKT 916

Query: 902  RSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 961
             SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK
Sbjct: 917  HSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 976

Query: 962  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1021
            VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL
Sbjct: 977  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1036

Query: 1022 ADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDP 1081
            ADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD 
Sbjct: 1037 ADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDA 1096

Query: 1082 EIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPD 1141
            EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPD
Sbjct: 1097 EIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPD 1156

Query: 1142 WLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSS 1201
            WLHKKVREKDDKFRQQKIV+MFASS G     N G S SK+VMN +NS+D+EDFQKKTSS
Sbjct: 1157 WLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQKKTSS 1216

Query: 1202 SSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHG 1231
             +GPKPVVR YDVR MKHSV R+SQ+DS MQEK      G  PSSPL+F++IDRN DY G
Sbjct: 1217 INGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNVDYRG 1276

BLAST of HG10022286 vs. NCBI nr
Match: XP_022137384.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Momordica charantia])

HSP 1 Score: 2229.5 bits (5776), Expect = 0.0e+00
Identity = 1113/1266 (87.91%), Postives = 1168/1266 (92.26%), Query Frame = 0

Query: 2    TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 61
            T  + +S +RRR+  D N+K KKQ I++S  AEE+IESKFGF LFSEGDKRLGWLLTF  
Sbjct: 17   TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76

Query: 62   SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 121
            SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77   SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136

Query: 122  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 181
            IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196

Query: 182  DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 241
            DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197  DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256

Query: 242  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 301
            DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316

Query: 302  YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 361
            YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317  YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376

Query: 362  KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 421
            KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN
Sbjct: 377  KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 436

Query: 422  PEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTL 481
            PEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+PDEVLRKGSGTL
Sbjct: 437  PEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTPDEVLRKGSGTL 496

Query: 482  CEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFR 541
            CEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESGVFRSD+PTSFR
Sbjct: 497  CEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESGVFRSDIPTSFR 556

Query: 542  LDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------- 601
            LDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK               
Sbjct: 557  LDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRLRDGPVREECPL 616

Query: 602  --------------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRS 661
                                PPS+VTDEVCTACDFN P KTCLR+LEWVW GEVF AK+S
Sbjct: 617  IYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS 676

Query: 662  DYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEV 721
            DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPVTEV
Sbjct: 677  DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEV 736

Query: 722  RKAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLY 781
            R+AGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAK SGNSIKIQEAQDM+VLY
Sbjct: 737  REAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDMLVLY 796

Query: 782  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 841
            DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT
Sbjct: 797  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 856

Query: 842  DGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEM 901
            DGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KIY+ 
Sbjct: 857  DGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKIYKT 916

Query: 902  RSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 961
             SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK
Sbjct: 917  HSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 976

Query: 962  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1021
            VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL
Sbjct: 977  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1036

Query: 1022 ADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDP 1081
            ADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD 
Sbjct: 1037 ADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDA 1096

Query: 1082 EIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPD 1141
            EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPD
Sbjct: 1097 EIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPD 1156

Query: 1142 WLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSS 1201
            WLHKKVREKDDKFRQQKIV+MFASS G     N G S SK+VMN +NS+D+EDFQKKTSS
Sbjct: 1157 WLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQKKTSS 1216

Query: 1202 SSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHG 1231
             +GPKPVVR YDVR MKHSV R+SQ+DS MQEK      G  PSSPL+F++IDRN DY G
Sbjct: 1217 INGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNVDYRG 1276

BLAST of HG10022286 vs. NCBI nr
Match: XP_038901114.1 (DNA polymerase epsilon catalytic subunit A-like isoform X1 [Benincasa hispida])

HSP 1 Score: 2220.3 bits (5752), Expect = 0.0e+00
Identity = 1107/1260 (87.86%), Postives = 1159/1260 (91.98%), Query Frame = 0

Query: 7    SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 66
            S  +RRR   D + + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF  SS +D+D
Sbjct: 2    SGESRRRGRTDKSSRSKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSLEDQD 61

Query: 67   TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 126
            T KV SCIDLYFVTQDGSSFKSKYKFRPYFY ATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62   TGKVNSCIDLYFVTQDGSSFKSKYKFRPYFYIATKDKMEIDVEAYLRRRYEGRIADIEIL 121

Query: 127  EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 186
            EKEDLDLKNHLSGL KAYLKLSFDTVQELMN+KSELLHVVESN  KSDAAEAYDSILFGK
Sbjct: 122  EKEDLDLKNHLSGLCKAYLKLSFDTVQELMNLKSELLHVVESNWAKSDAAEAYDSILFGK 181

Query: 187  REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 246
            REQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182  REQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241

Query: 247  VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 306
            V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242  VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 301

Query: 307  QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 366
            +GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KM DEVG
Sbjct: 302  EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMIDEVG 361

Query: 367  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 426
            FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF
Sbjct: 362  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 421

Query: 427  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 486
            AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV
Sbjct: 422  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 481

Query: 487  QAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 546
            QAY ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYD
Sbjct: 482  QAYNANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYD 541

Query: 547  QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK---------------------- 606
            QLI NLDRDLQYAIQVEGKMDLELVSNYSDVK AI EK                      
Sbjct: 542  QLIYNLDRDLQYAIQVEGKMDLELVSNYSDVKNAILEKLIRLRDEPVREEGPLIYHLDVA 601

Query: 607  -------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKR 666
                         PPS+VTDE+CTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+
Sbjct: 602  AMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKK 661

Query: 667  QIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICM 726
            QIESEFVDSG VRLSK FLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVR+AGICM
Sbjct: 662  QIESEFVDSGNVRLSKSFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVREAGICM 721

Query: 727  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH 786
            RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH
Sbjct: 722  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH 781

Query: 787  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 846
            KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL
Sbjct: 782  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 841

Query: 847  PGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIE 906
            PGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+  SECSIE
Sbjct: 842  PGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSECSIE 901

Query: 907  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 966
            FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF
Sbjct: 902  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 961

Query: 967  DKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQK 1026
            DKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DYGEQK
Sbjct: 962  DKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDYGEQK 1021

Query: 1027 SCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYV 1086
            SCAVTTA+RLADFLGDTMV+DKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYV
Sbjct: 1022 SCAVTTARRLADFLGDTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYV 1081

Query: 1087 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVR 1146
            RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVR
Sbjct: 1082 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVR 1141

Query: 1147 EKDDKFRQQKIVNMFASS-RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKP 1206
            EKDDKFRQQK+ +MFASS R KY + N   S SK+ MN  NS+D+EDFQKKTS  +GPKP
Sbjct: 1142 EKDDKFRQQKVADMFASSNRVKYSKKNSDVSCSKHAMNEDNSEDLEDFQKKTSPRNGPKP 1201

Query: 1207 VVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            VVR YDVR MKHS+T +S+++S MQE F   + G +PSSPLH ++ID+N DY GWLEMKK
Sbjct: 1202 VVRCYDVRGMKHSLTSNSEINSEMQENFN--DGGCEPSSPLHVENIDKNVDYSGWLEMKK 1259

BLAST of HG10022286 vs. NCBI nr
Match: XP_038901115.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Benincasa hispida])

HSP 1 Score: 2220.3 bits (5752), Expect = 0.0e+00
Identity = 1107/1260 (87.86%), Postives = 1159/1260 (91.98%), Query Frame = 0

Query: 7    SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 66
            S  +RRR   D + + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF  SS +D+D
Sbjct: 2    SGESRRRGRTDKSSRSKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSLEDQD 61

Query: 67   TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 126
            T KV SCIDLYFVTQDGSSFKSKYKFRPYFY ATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62   TGKVNSCIDLYFVTQDGSSFKSKYKFRPYFYIATKDKMEIDVEAYLRRRYEGRIADIEIL 121

Query: 127  EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 186
            EKEDLDLKNHLSGL KAYLKLSFDTVQELMN+KSELLHVVESN  KSDAAEAYDSILFGK
Sbjct: 122  EKEDLDLKNHLSGLCKAYLKLSFDTVQELMNLKSELLHVVESNWAKSDAAEAYDSILFGK 181

Query: 187  REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 246
            REQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182  REQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241

Query: 247  VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 306
            V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242  VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 301

Query: 307  QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 366
            +GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KM DEVG
Sbjct: 302  EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMIDEVG 361

Query: 367  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 426
            FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF
Sbjct: 362  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 421

Query: 427  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 486
            AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV
Sbjct: 422  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 481

Query: 487  QAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 546
            QAY ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYD
Sbjct: 482  QAYNANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYD 541

Query: 547  QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK---------------------- 606
            QLI NLDRDLQYAIQVEGKMDLELVSNYSDVK AI EK                      
Sbjct: 542  QLIYNLDRDLQYAIQVEGKMDLELVSNYSDVKNAILEKLIRLRDEPVREEGPLIYHLDVA 601

Query: 607  -------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKR 666
                         PPS+VTDE+CTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+
Sbjct: 602  AMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKK 661

Query: 667  QIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICM 726
            QIESEFVDSG VRLSK FLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVR+AGICM
Sbjct: 662  QIESEFVDSGNVRLSKSFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVREAGICM 721

Query: 727  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH 786
            RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH
Sbjct: 722  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH 781

Query: 787  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 846
            KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL
Sbjct: 782  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 841

Query: 847  PGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIE 906
            PGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+  SECSIE
Sbjct: 842  PGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSECSIE 901

Query: 907  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 966
            FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF
Sbjct: 902  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 961

Query: 967  DKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQK 1026
            DKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DYGEQK
Sbjct: 962  DKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDYGEQK 1021

Query: 1027 SCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYV 1086
            SCAVTTA+RLADFLGDTMV+DKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYV
Sbjct: 1022 SCAVTTARRLADFLGDTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYV 1081

Query: 1087 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVR 1146
            RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVR
Sbjct: 1082 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVR 1141

Query: 1147 EKDDKFRQQKIVNMFASS-RGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKP 1206
            EKDDKFRQQK+ +MFASS R KY + N   S SK+ MN  NS+D+EDFQKKTS  +GPKP
Sbjct: 1142 EKDDKFRQQKVADMFASSNRVKYSKKNSDVSCSKHAMNEDNSEDLEDFQKKTSPRNGPKP 1201

Query: 1207 VVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            VVR YDVR MKHS+T +S+++S MQE F   + G +PSSPLH ++ID+N DY GWLEMKK
Sbjct: 1202 VVRCYDVRGMKHSLTSNSEINSEMQENFN--DGGCEPSSPLHVENIDKNVDYSGWLEMKK 1259

BLAST of HG10022286 vs. NCBI nr
Match: XP_022153062.1 (DNA polymerase epsilon catalytic subunit A-like isoform X2 [Momordica charantia])

HSP 1 Score: 2214.5 bits (5737), Expect = 0.0e+00
Identity = 1106/1255 (88.13%), Postives = 1155/1255 (92.03%), Query Frame = 0

Query: 12   RRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVY 71
            RR  R +N + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF  SS +D+DT KV+
Sbjct: 6    RRRGRGDNTRLKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSWEDQDTGKVF 65

Query: 72   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDL 131
            SCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+GQIADIEILEKEDL
Sbjct: 66   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGQIADIEILEKEDL 125

Query: 132  DLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRL 191
            DLKNHLSGL KAYLKLSFDTVQELM+VKSELLHVVESN  KSDAAEAYDSILFGKREQ+ 
Sbjct: 126  DLKNHLSGLCKAYLKLSFDTVQELMHVKSELLHVVESNWAKSDAAEAYDSILFGKREQKP 185

Query: 192  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICA 251
            QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+ LEKRKDLLQRAEV +CA
Sbjct: 186  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVMLEKRKDLLQRAEVHVCA 245

Query: 252  FDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFK 311
            FDIETTKLPLKFPDAEYDMIMMISYM+DGQGYLIINRECVGEDIENLEYTPKPEY+GFF+
Sbjct: 246  FDIETTKLPLKFPDAEYDMIMMISYMIDGQGYLIINRECVGEDIENLEYTPKPEYEGFFR 305

Query: 312  VNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDK 371
            V NVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHG KMSDEVGFQCDK
Sbjct: 306  VTNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSDEVGFQCDK 365

Query: 372  NQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKP 431
            NQGECRSKFACHLDCFAWVKRDSYLPQGS GLKAVTKAKLGYDPLEVNPEDMVR AKEKP
Sbjct: 366  NQGECRSKFACHLDCFAWVKRDSYLPQGSQGLKAVTKAKLGYDPLEVNPEDMVRSAKEKP 425

Query: 432  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEA 491
            QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAY+A
Sbjct: 426  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYKA 485

Query: 492  NVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINN 551
            NVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYDQLI N
Sbjct: 486  NVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYDQLIYN 545

Query: 552  LDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------------------- 611
            LDRDLQYAIQVEGKMDLELV NY+DVK AI EK                           
Sbjct: 546  LDRDLQYAIQVEGKMDLELVLNYNDVKNAIMEKLIRLRDEPVREEGPLIYHLDVAAMYPN 605

Query: 612  --------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESE 671
                    PPS+VTDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+QIESE
Sbjct: 606  IILTNRLQPPSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKKQIESE 665

Query: 672  FVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSF 731
            FVDSG VRLSK FLELPKLEQQ KLKERLKKYCQKAYKRVLDKPVTEVR+AGICMRENSF
Sbjct: 666  FVDSGNVRLSKSFLELPKLEQQTKLKERLKKYCQKAYKRVLDKPVTEVREAGICMRENSF 725

Query: 732  YIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 791
            YIDTVRSFRDRRYEYKGLNKVWKG+LSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN
Sbjct: 726  YIDTVRSFRDRRYEYKGLNKVWKGRLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 785

Query: 792  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFP 851
            SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNA LLIEKIGKPLELDTDGIWCALPGSFP
Sbjct: 786  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNACLLIEKIGKPLELDTDGIWCALPGSFP 845

Query: 852  ENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDG 911
            ENFTFKTK+ KKLTISYPCVMLNVDVARNNTNDQYQTL+DPVRKIY+  SECSIEFEVDG
Sbjct: 846  ENFTFKTKDLKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVRKIYKTHSECSIEFEVDG 905

Query: 912  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 971
            PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH
Sbjct: 906  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 965

Query: 972  GSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1031
            GSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT
Sbjct: 966  GSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1025

Query: 1032 TAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCK 1091
            TA+RLADFLGD MVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYVRKWCK
Sbjct: 1026 TARRLADFLGDAMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYVRKWCK 1085

Query: 1092 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDK 1151
            ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVREKDDK
Sbjct: 1086 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKDDK 1145

Query: 1152 FRQQKIVNMFAS-SRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKPVVRSY 1211
            FRQQK+ +MFAS +RGKY   N     SK+VMN +NS+D+EDFQKK SS++GPKPVVR Y
Sbjct: 1146 FRQQKVADMFASLNRGKYSGKNSDDGCSKHVMNEENSEDLEDFQKKASSTNGPKPVVRCY 1205

Query: 1212 DVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            DVR MKH   RDS+VDS+ Q  F G   G +PSSPLH +++D+N DYHGWLEMKK
Sbjct: 1206 DVRGMKH--PRDSEVDSVRQVNFNG--GGCEPSSPLHVENVDKNVDYHGWLEMKK 1256

BLAST of HG10022286 vs. ExPASy Swiss-Prot
Match: F4HW04 (DNA polymerase epsilon catalytic subunit A OS=Arabidopsis thaliana OX=3702 GN=POL2A PE=1 SV=1)

HSP 1 Score: 1968.7 bits (5099), Expect = 0.0e+00
Identity = 972/1261 (77.08%), Postives = 1094/1261 (86.76%), Query Frame = 0

Query: 7    SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 66
            S  NRRRD +D  +  KK  ++ +AE+++ESK GF LFSEG+ RLGWLLTF+SSS +D D
Sbjct: 2    SGDNRRRDRKDTRWS-KKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRD 61

Query: 67   TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 126
            T KVYSC+DLYFVTQDG SFK+KYKFRPYFYAATK+KME ++EAYLRRRY+ Q+ADIEI+
Sbjct: 62   TGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIV 121

Query: 127  EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 186
            EKEDLDLKNHLSGL K YLK+SFDTVQ+LM VK +LLH+VE N+ K DA EAY+SIL GK
Sbjct: 122  EKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGK 181

Query: 187  REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 246
            REQR QD L+ IVDLREYDVPYHVRFAID+D+R GQWY+VS+SST + LEKR DLLQRAE
Sbjct: 182  REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAE 241

Query: 247  VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 306
            VR+CAFDIETTKLPLKFPDAEYD IMMISYMVDGQG+LIINRECVGED+E+LEYTPKPE+
Sbjct: 242  VRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEF 301

Query: 307  QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 366
            +G+FKV NVKNE+ELL+ WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+G
Sbjct: 302  EGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELG 361

Query: 367  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 426
            F+CD+NQGECR+KFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF
Sbjct: 362  FRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 421

Query: 427  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 486
            A EKPQ MASYSVSDAVATYYLYMTYV+PFIFSLATIIPM PDEVLRKGSGTLCEMLLMV
Sbjct: 422  AMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKGSGTLCEMLLMV 481

Query: 487  QAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 546
            +AY+ANVVCPNK+Q+DPEKF+ N+LLESETYIGGHVECLESGVFRSD+PTSF+LDSSAY 
Sbjct: 482  EAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIPTSFKLDSSAYQ 541

Query: 547  QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK---------------------- 606
            QLI+NL RDL+YAI VEGKM ++ +SNY +VK  I EK                      
Sbjct: 542  QLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVA 601

Query: 607  -------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKR 666
                         PPS+VTDE+CTACDFNRPGKTCLR+LEWVW G  F  K+SDYYHLK+
Sbjct: 602  AMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKSDYYHLKK 661

Query: 667  QIESEFVDSG-TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGIC 726
            QIESEFVD+G  +  SK FL+LPK++QQ+KLKERLKKYCQKAYKRVLDKP+TEVR+AGIC
Sbjct: 662  QIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREAGIC 721

Query: 727  MRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLA 786
            MREN FY+DTVRSFRDRRYEYK LNKVWKGKLSEAKASGNSIKIQEAQDMVV+YDSLQLA
Sbjct: 722  MRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSLQLA 781

Query: 787  HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCA 846
            HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWC 
Sbjct: 782  HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCC 841

Query: 847  LPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSI 906
            LPGSFPENFTFKT + KKLTISYPCVMLNVDVA+NNTNDQYQTLVDPVRK Y+  SECSI
Sbjct: 842  LPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSECSI 901

Query: 907  EFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAEL 966
            EFEVDGPYKAMI+PASKEEGILIKKRYAVFN DGTLAELKGFEIKRRGELKLIKVFQAEL
Sbjct: 902  EFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQAEL 961

Query: 967  FDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQ 1026
            FDKFLHGSTLEECYS VA+VA+RWLDLLD+QGKDIADSELLDYISESSTMSKSLADYGEQ
Sbjct: 962  FDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQ 1021

Query: 1027 KSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFY 1086
            KSCAVTTAKRLA+FLG TMV+DKGLRCQYIVACEPKGTPVSERAVPVAIF T+PE+MKF+
Sbjct: 1022 KSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEVMKFH 1081

Query: 1087 VRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKV 1146
            +RKWCK SS+VGIR IIDWSYYKQRLSSAIQK+ITIPAAMQKV+NPVPRV+HPDWLHKKV
Sbjct: 1082 LRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWLHKKV 1141

Query: 1147 REKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPK 1206
            REKDDKFRQ+K+V+MF+S+      N D    +   +   N +DIEDF K+   S  GPK
Sbjct: 1142 REKDDKFRQRKLVDMFSSA------NKDVVLDTDLPVTKDNVEDIEDFCKENRPSVKGPK 1201

Query: 1207 PVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMK 1231
            P+ RSY+V              +  Q + +  ES       + F++ID++ +Y GWLE+K
Sbjct: 1202 PIARSYEV--------------NKKQSECEQQESWDTEFHDISFQNIDKSVNYQGWLELK 1241

BLAST of HG10022286 vs. ExPASy Swiss-Prot
Match: F4IFN6 (DNA polymerase epsilon catalytic subunit B OS=Arabidopsis thaliana OX=3702 GN=POL2B PE=2 SV=1)

HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 924/1238 (74.64%), Postives = 1049/1238 (84.73%), Query Frame = 0

Query: 30   SAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSK 89
            SA +++E+K GF LFS+G+ RLGWLLTF SSS +D DT K +SC+DL+FVTQDGSSFK+K
Sbjct: 16   SAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTK 75

Query: 90   YKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSF 149
            YKFRPY YAATK+ ME +VEAYLRRRY+ Q+ADI+I+ KEDL LKNHLSGL K YLK+SF
Sbjct: 76   YKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSF 135

Query: 150  DTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQDFLECIVDLREYDVPYH 209
            DTVQ+L+ VK +LLH+VE N  K +A EAY+SIL GKREQR QD L+ +VDLREYDVPYH
Sbjct: 136  DTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYH 195

Query: 210  VRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYD 269
            VRFAID+D+R GQWY+VS+SST + LEKR DLLQRAEVR+CAFDIET KLPLKFPDAEYD
Sbjct: 196  VRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYD 255

Query: 270  MIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHM 329
             IMMISYMVDGQG+LI NRECVG+DIE+LEYTPKPE++G+FKV NV NE+ELL+ WF+HM
Sbjct: 256  QIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHM 315

Query: 330  REVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRSKFACHLDCFAW 389
            +E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+GF+CD+NQGECR+KF CHLDCF+W
Sbjct: 316  QELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSW 375

Query: 390  VKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLY 449
            VKRDSYLPQGS GLKAVTK KLGYDPLEVNPEDMVRFA EKPQ MASYSVSDAVATYYLY
Sbjct: 376  VKRDSYLPQGSQGLKAVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLY 435

Query: 450  MTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNN 509
            MTYVHPF+FSLATIIPM PDEVLRKGSGTLCEMLLMV+AY+ANVVCPNK+Q+DPEKF+  
Sbjct: 436  MTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQG 495

Query: 510  RLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLE 569
            +LLESETYIGGHVECL+SGVFRSD+PTSF+LD+SAY QLI+NL RDL+YAI VEGKM ++
Sbjct: 496  KLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEYAITVEGKMRMD 555

Query: 570  LVSNYSDVKTAITEK-----------------------------------PPSVVTDEVC 629
             VSN+ +VK  I EK                                   PPS+VTDEVC
Sbjct: 556  SVSNFDEVKEVIREKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEVC 615

Query: 630  TACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESEFVDSG-TVRLSKQFLELP 689
            TACDFN P KTCLR+LEWVW G  F+  +S+YYHLK+QIESE VD+G  ++ SK FL+LP
Sbjct: 616  TACDFNGPEKTCLRKLEWVWRGVTFKGNKSEYYHLKKQIESESVDAGANMQSSKPFLDLP 675

Query: 690  KLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTVRSFRDRRYEYKG 749
            K+EQQ+KLKERLKKYCQKAY RVLDKP+TEVR+AGICMREN FY+DTVRSFRDRRYEYK 
Sbjct: 676  KVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKT 735

Query: 750  LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSME 809
            LNKVWKGKLSEAKASGN IKIQEA DMVV+YDSLQLAHKCILNSFYGYVMRKGARWYSME
Sbjct: 736  LNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSME 795

Query: 810  MAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTFKTKESKKLTISY 869
            MAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWCALPGSFPENFTFKT + KK TISY
Sbjct: 796  MAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFTISY 855

Query: 870  PCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYKAMILPASKEEGILI 929
            PCV+LNVDVA+NN+NDQYQTLVDPVRK Y  RSECSIEFEVDGPYKAMI+PASKEEGILI
Sbjct: 856  PCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEGILI 915

Query: 930  KKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEECYSVVASVANR 989
            KKRYAVFN DGT+AELKGFE+KRRGELKLIKVFQAELFDKFLHGSTLEECYS VA+VANR
Sbjct: 916  KKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVANR 975

Query: 990  WLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLADFLGDTMVRDK 1049
            WLDLL+ QGKDIADSELLDYISESSTMSKSLADYG+QKSCAVTTAKRLADFLGDTMV+DK
Sbjct: 976  WLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTMVKDK 1035

Query: 1050 GLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYK 1109
            GLRCQYIVA EP+GTPVSERAVPVAIF+TD    KFY++KWCKISS  GIRSIIDW YYK
Sbjct: 1036 GLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDWMYYK 1095

Query: 1110 QRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKFRQQKIVNMFASSRGKY 1169
            QRL SAIQK+ITIPAAMQKV+NPV RV HP WL KKV    DKFRQ KIV+MF+S+    
Sbjct: 1096 QRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKV---CDKFRQGKIVDMFSSA---- 1155

Query: 1170 LENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPKPVVRSYDVRAMKHSVTRDSQVDS 1229
              N D +++  NV+      DIE+F K+   S  GPKPV RS++V    HS         
Sbjct: 1156 --NKDHSTTQDNVV-----ADIEEFCKENRPSVKGPKPVARSFEV-DRNHS--------- 1215

Query: 1230 MMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
                + K  ES       +  +++D+N DY GWLE++K
Sbjct: 1216 ----EGKQQESWDPEFHDISLQNVDKNVDYQGWLELEK 1225

BLAST of HG10022286 vs. ExPASy Swiss-Prot
Match: Q07864 (DNA polymerase epsilon catalytic subunit A OS=Homo sapiens OX=9606 GN=POLE PE=1 SV=5)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 634/1181 (53.68%), Postives = 832/1181 (70.45%), Query Frame = 0

Query: 33   EDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKF 92
            + ++ +FGF    E  ++ GWL+    +   DED +++ S +D YF+  DGS FK    +
Sbjct: 42   DKMDLRFGFERLKEPGEKTGWLINMHPTEILDED-KRLGSAVDYYFIQDDGSRFKVALPY 101

Query: 93   RPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTV 152
            +PYFY AT+   E +V ++L ++++G+IA +E + KEDLDL NHL GL + Y++LSF TV
Sbjct: 102  KPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTV 161

Query: 153  QELMNVKSELLHVVESNREKSDAAEAYDSILFG------------KREQRLQDFLECIVD 212
            ++L+ V+ E+   V+ NRE+  A++AY ++L              +  +++ D L+ IVD
Sbjct: 162  EDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVD 221

Query: 213  LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLE--KRKDLLQRAEVRICAFDIETTK 272
            +REYDVPYH+R +ID  +    WY+V        +E  +R DL++R +  + AFDIETTK
Sbjct: 222  MREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTK 281

Query: 273  LPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNE 332
            LPLKFPDAE D IMMISYM+DGQGYLI NRE V EDIE+ E+TPKPEY+G F V N  +E
Sbjct: 282  LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDE 341

Query: 333  LELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRS 392
              L++ WF H++E KP I VTYNGD+FDWPF+E+RAA HGL M  E+GFQ D +QGE ++
Sbjct: 342  AHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKD-SQGEYKA 401

Query: 393  KFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYS 452
                H+DC  WVKRDSYLP GSH LKA  KAKLGYDP+E++PEDM R A E+PQ +A+YS
Sbjct: 402  PQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYS 461

Query: 453  VSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNK 512
            VSDAVATYYLYM YVHPFIF+L TIIPM PDEVLRKGSGTLCE LLMVQA+ AN++ PNK
Sbjct: 462  VSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNK 521

Query: 513  HQSDPEKF-HNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQ 572
             + +  K   +  +L+SETY+GGHVE LESGVFRSD+P  FR++ +A+D L+  +++ L+
Sbjct: 522  QEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLR 581

Query: 573  YAIQVEGKMDLELVSNYSDVKTAITEK--------------------------------- 632
            +A++ E K+ +E V+N+ +V   I  K                                 
Sbjct: 582  HALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNR 641

Query: 633  --PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESE-FVDSG 692
              P ++V +  C ACDFN+PG  C R++ W W GE   A RS+Y+ ++ Q+ESE F    
Sbjct: 642  LQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLF 701

Query: 693  TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTV 752
                ++ F EL + EQ    K RL  YC+KAYK++    V E R   IC RENSFY+DTV
Sbjct: 702  PEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEE-RLTTICQRENSFYVDTV 761

Query: 753  RSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGY 812
            R+FRDRRYE+KGL+KVWK KLS A   G++ +++  ++M VLYDSLQLAHKCILNSFYGY
Sbjct: 762  RAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGY 821

Query: 813  VMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTF 872
            VMRKGARWYSMEMAG+V +TGA II  AR LIE+IG+PLELDTDGIWC LP SFPENF F
Sbjct: 822  VMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVF 881

Query: 873  KTKESK--KLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYK 932
            KT   K  K+TISYP  MLN+ V    TNDQYQ L +P    Y  RSE SI FEVDGPY 
Sbjct: 882  KTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYL 941

Query: 933  AMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGST 992
            AMILPASKEEG  +KKRYAVFN+DG+LAELKGFE+KRRGEL+LIK+FQ+ +F+ FL GST
Sbjct: 942  AMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGST 1001

Query: 993  LEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAK 1052
            LEE Y  VA VA+ WLD+L S+  ++ DSEL + ISE+ +MS+ L DYGEQKS +++TAK
Sbjct: 1002 LEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAK 1061

Query: 1053 RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISS 1112
            RLA+FLGD MV+D GL C+YI++ +P+G+PV+ERA+P+AIF+ +P + K ++RKW K SS
Sbjct: 1062 RLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSS 1121

Query: 1113 --EVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKF 1159
              +  IR+I+DW YY +RL SAIQKIITIPAA+Q+V NPVPRV HPDWLHKK+ EK+D +
Sbjct: 1122 LQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVY 1181

BLAST of HG10022286 vs. ExPASy Swiss-Prot
Match: Q9WVF7 (DNA polymerase epsilon catalytic subunit A OS=Mus musculus OX=10090 GN=Pole PE=1 SV=3)

HSP 1 Score: 1248.4 bits (3229), Expect = 0.0e+00
Identity = 627/1149 (54.57%), Postives = 821/1149 (71.45%), Query Frame = 0

Query: 33   EDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKF 92
            + ++ +FGF    E  +R GWL+    +   DED +++ S +D YF+  DGS FK    +
Sbjct: 42   DKMDLRFGFERLKEPGERTGWLINMHPTEILDED-KRLVSAVDYYFIQDDGSRFKVALPY 101

Query: 93   RPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTV 152
             PYFY A +   + +V ++L ++++G+IA +E + KEDLDL NHL GL ++Y+KLSF TV
Sbjct: 102  MPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTV 161

Query: 153  QELMNVKSELLHVVESNREKSDAAEAY----DSILFG--------KREQRLQDFLECIVD 212
            ++L+ V+ E+   V+ NRE+  A++ Y     SIL G        +  +++ D L+ IVD
Sbjct: 162  EDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNIVD 221

Query: 213  LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLE--KRKDLLQRAEVRICAFDIETTK 272
            +REYDVPYH+R +ID  +    WY+V        +E  +R DL++R +  + AFDIETTK
Sbjct: 222  MREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTK 281

Query: 273  LPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNE 332
            LPLKFPDAE D IMMISYM+DGQGYLI NRE V EDIE+ E+TPKPEY+G F V N  +E
Sbjct: 282  LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDE 341

Query: 333  LELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRS 392
            + L++ WF H++E KP I VTYNGD+FDWPF+E+RAA HGL M  E+GFQ D +QGE ++
Sbjct: 342  VHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKD-SQGEYKA 401

Query: 393  KFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYS 452
                H+DC  WVKRDSYLP GSH LKA  KAKLGYDP+E++PEDM R A E+PQ +A+YS
Sbjct: 402  PQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYS 461

Query: 453  VSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNK 512
            VSDAVATYYLYM YVHPFIF+L TIIPM PDEVLRKGSGTLCE LLMVQA+ AN++ PNK
Sbjct: 462  VSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNK 521

Query: 513  HQSDPEKF-HNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQ 572
             + +  K   +  +L++ETY+GGHVE LESGVFRSD+P  FR++ +A+D L+  +++ ++
Sbjct: 522  QEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMR 581

Query: 573  YAIQVEGKMDLELVSNYSDV----KTAITE------------------------------ 632
            +AI+ E K+ +E  +N+ +V    KT +T                               
Sbjct: 582  HAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNR 641

Query: 633  -KPPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESE-FVDSG 692
             +P ++V +  C ACDFN+PG +C R++ W W GE   A RS+Y+ ++ Q+ESE F    
Sbjct: 642  LQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLF 701

Query: 693  TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTV 752
                ++ F EL + EQ    K RL  YC+KAYK++    V E R   IC RENSFY+DTV
Sbjct: 702  PEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEE-RLTTICQRENSFYVDTV 761

Query: 753  RSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGY 812
            R+FRDRRYE+KGL+KVWK KLS A   G++ +++  ++M +LYDSLQLAHKCILNSFYGY
Sbjct: 762  RAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGY 821

Query: 813  VMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTF 872
            VMRKGARWYSMEMAG+V +TGA II  AR LIE+IG+PLELDTDGIWC LP SFPENF  
Sbjct: 822  VMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVI 881

Query: 873  KTKESK--KLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYK 932
            KT  +K  KLTISYP  MLN+ V    TN QYQ L +P    Y   SE SI FEVDGPY 
Sbjct: 882  KTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTHSENSIFFEVDGPYL 941

Query: 933  AMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGST 992
            AMILPASKEEG  +KKRYAVFN+DG+LAELKGFE+KRRGEL+LIK+FQ+ +F+ FL GST
Sbjct: 942  AMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGST 1001

Query: 993  LEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAK 1052
            LEE Y  VA VA+ WLD+L S+  ++ DSEL + ISE+ +MS+ L DYGEQKS +++TAK
Sbjct: 1002 LEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAK 1061

Query: 1053 RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISS 1112
            RLA+FLGD MV+D GL C+YI++ +P+G+PV+ERA+P+AIF+ +P + K ++RKW K SS
Sbjct: 1062 RLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSS 1121

Query: 1113 --EVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKF 1127
              +  IR+I+DW YY +RL SAIQKIITIPAA+Q+V NPVPRV HPDWLHKK+ EK+D +
Sbjct: 1122 LQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIY 1181

BLAST of HG10022286 vs. ExPASy Swiss-Prot
Match: Q4WXH8 (DNA polymerase epsilon catalytic subunit A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=pol2 PE=3 SV=1)

HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 619/1155 (53.59%), Postives = 800/1155 (69.26%), Query Frame = 0

Query: 35   IESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRP 94
            I+   GF  F  G+KR+GWL+   S+S +D +     + +D YF+   G SFK+  ++ P
Sbjct: 54   IDEALGFPRFESGEKRVGWLINMHSTSIEDPNVPGGRAGVDYYFLEDGGGSFKATVEYDP 113

Query: 95   YFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQE 154
            YF  A K   E +VE + RR ++G I  I+ +EKEDL L NHL G  + +L+L+F  V  
Sbjct: 114  YFLLAVKKGHEAEVEEWCRRMFEGLIKTIKRVEKEDLQLPNHLLGHRRTFLQLNFANVSH 173

Query: 155  LMNVKSELLHVVESNREKSDAAEAY--------------DSILFGKREQRLQDFLECIVD 214
            L++V+  LL + E N++  +  + Y              D ++   R     +  + I+D
Sbjct: 174  LLDVRKTLLPLAEKNKKNVNVMDTYAEISSANAGFDLFDDELINESRPNSNMNASDYIID 233

Query: 215  LREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLP 274
            +REYDVPYHVR AID D+R G+WY V      I+L   ++ LQRA+  I AFDIETTKLP
Sbjct: 234  IREYDVPYHVRVAIDKDIRIGKWYTVEAKHGVISLTCLEERLQRADPVILAFDIETTKLP 293

Query: 275  LKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELE 334
            LKFPD+  D IMMISYM+DGQG+LI NRE V EDI + EYTPKPEY G F + N  NE  
Sbjct: 294  LKFPDSVIDQIMMISYMIDGQGFLITNREIVSEDIRDFEYTPKPEYSGPFMIFNEPNERS 353

Query: 335  LLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGEC-RSK 394
            +++ +F H++E KP +  TYNGD+FDWPF+E+RA+  G+ M  E+GF+  KN  +  +S 
Sbjct: 354  VIERFFEHIKEAKPTVIATYNGDFFDWPFVEARASVLGIDMYKEIGFR--KNSEDIYQSD 413

Query: 395  FACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSV 454
               H+DCFAWV RDSYLPQGS GLKAVT AKLGYDP E++PE M  +A E+PQ +A YSV
Sbjct: 414  HCVHMDCFAWVNRDSYLPQGSRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSV 473

Query: 455  SDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNKH 514
            SDAVATYYLYM YVHPFIFSL TIIP++PD+ LRKG+GTLCEMLLMVQAY+  +V PNKH
Sbjct: 474  SDAVATYYLYMKYVHPFIFSLCTIIPLNPDDTLRKGTGTLCEMLLMVQAYKGEIVLPNKH 533

Query: 515  QSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQYA 574
            +  PE F+   LLESETY+GGHVE +E+GVFRSD+P +F +D++A D+L+ +LD  L+++
Sbjct: 534  KDPPESFYEGHLLESETYVGGHVESIEAGVFRSDIPVTFNIDTTAIDELLRDLDAALKFS 593

Query: 575  IQVEGKMDLELVSNYSDVKTAITEK----------------------------------- 634
            I+VE K  ++ V NY DVK  I E+                                   
Sbjct: 594  IEVEEKKSMDDVVNYEDVKAQIAERLVNLREKPHRDEVPSIYHLDVASMYPNIMITNRLQ 653

Query: 635  PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESEFVDSGTVR 694
            P S++ +  C ACDFNRPGKTC R L W W GE   AKR +Y  +++ + +E     T  
Sbjct: 654  PDSMIQESDCAACDFNRPGKTCDRRLPWAWRGEFLPAKRDEYNMIRQAVANELFPGRTKN 713

Query: 695  L-SKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTVRS 754
               + F E+   EQ A +K+RL+ Y +K Y ++ D   T VR+A IC REN FY+DTVRS
Sbjct: 714  SPMRSFGEMSAEEQAAIIKKRLQDYSKKIYHKIHDSK-TIVREAIICQRENPFYVDTVRS 773

Query: 755  FRDRRYEYKGLNKVWKGKLSEAKASG-NSIKIQEAQDMVVLYDSLQLAHKCILNSFYGYV 814
            FRDRRY++KG  KVWKGK    KA+G ++ +I+EA+ M+VL+DSLQLAHK ILNSFYGYV
Sbjct: 774  FRDRRYDFKGKQKVWKGKTEALKAAGASAAEIEEAKKMIVLFDSLQLAHKVILNSFYGYV 833

Query: 815  MRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTFK 874
            MRKG+RWYSMEMAGV   TGA IIQ AR L+E+IG+PLELDTDGIWC LPGSFPE+F+F 
Sbjct: 834  MRKGSRWYSMEMAGVTCLTGAHIIQMARELVERIGRPLELDTDGIWCMLPGSFPEDFSFT 893

Query: 875  TKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYKAMI 934
             K  KKL ISYPCVMLN  V    TN QYQTLVDP    YE  S+ SI FEVDGPYKAMI
Sbjct: 894  LKNGKKLGISYPCVMLNHLVHGKYTNHQYQTLVDPKTFRYETHSDNSIFFEVDGPYKAMI 953

Query: 935  LPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEE 994
            LP SKEE   +KKRYAVFN DG+LAELKGFE+KRRGELKLIK+FQ ++F  FL GSTLEE
Sbjct: 954  LPTSKEEDKNLKKRYAVFNHDGSLAELKGFEVKRRGELKLIKIFQTQIFRFFLEGSTLEE 1013

Query: 995  CYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLA 1054
             Y+ VA VA+RWLD+L   G  +AD EL++ ISE+ +M+K+L +YG QKS ++TTA+RLA
Sbjct: 1014 TYAAVARVADRWLDVLYDHGATLADEELIELISENRSMTKTLEEYGNQKSTSITTARRLA 1073

Query: 1055 DFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKIS-SEV 1114
            +FLG+ MV+DKGL C+YI++ +PK TPV+ERA+PV IF  +  + +F++RKW K    ++
Sbjct: 1074 EFLGEQMVKDKGLNCKYIISAKPKNTPVTERAIPVTIFSAEEPVKRFFLRKWLKDDPGDM 1133

Query: 1115 GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKFRQQK 1137
              RS+IDW YY +RL S +QK+ITIPAA+QKV NPVPRV HPDWL +++  K+DKF+Q K
Sbjct: 1134 DPRSVIDWDYYLERLGSVVQKLITIPAALQKVRNPVPRVAHPDWLQRRINMKEDKFKQTK 1193

BLAST of HG10022286 vs. ExPASy TrEMBL
Match: A0A6J1C732 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111008850 PE=3 SV=1)

HSP 1 Score: 2229.5 bits (5776), Expect = 0.0e+00
Identity = 1113/1266 (87.91%), Postives = 1168/1266 (92.26%), Query Frame = 0

Query: 2    TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 61
            T  + +S +RRR+  D N+K KKQ I++S  AEE+IESKFGF LFSEGDKRLGWLLTF  
Sbjct: 17   TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76

Query: 62   SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 121
            SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77   SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136

Query: 122  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 181
            IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196

Query: 182  DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 241
            DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197  DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256

Query: 242  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 301
            DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316

Query: 302  YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 361
            YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317  YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376

Query: 362  KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 421
            KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN
Sbjct: 377  KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 436

Query: 422  PEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTL 481
            PEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+PDEVLRKGSGTL
Sbjct: 437  PEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTPDEVLRKGSGTL 496

Query: 482  CEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFR 541
            CEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESGVFRSD+PTSFR
Sbjct: 497  CEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESGVFRSDIPTSFR 556

Query: 542  LDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------- 601
            LDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK               
Sbjct: 557  LDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRLRDGPVREECPL 616

Query: 602  --------------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRS 661
                                PPS+VTDEVCTACDFN P KTCLR+LEWVW GEVF AK+S
Sbjct: 617  IYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS 676

Query: 662  DYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEV 721
            DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPVTEV
Sbjct: 677  DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEV 736

Query: 722  RKAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLY 781
            R+AGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAK SGNSIKIQEAQDM+VLY
Sbjct: 737  REAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDMLVLY 796

Query: 782  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 841
            DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT
Sbjct: 797  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 856

Query: 842  DGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEM 901
            DGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KIY+ 
Sbjct: 857  DGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKIYKT 916

Query: 902  RSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 961
             SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK
Sbjct: 917  HSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 976

Query: 962  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1021
            VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL
Sbjct: 977  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1036

Query: 1022 ADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDP 1081
            ADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD 
Sbjct: 1037 ADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDA 1096

Query: 1082 EIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPD 1141
            EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPD
Sbjct: 1097 EIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPD 1156

Query: 1142 WLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSS 1201
            WLHKKVREKDDKFRQQKIV+MFASS G     N G S SK+VMN +NS+D+EDFQKKTSS
Sbjct: 1157 WLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQKKTSS 1216

Query: 1202 SSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHG 1231
             +GPKPVVR YDVR MKHSV R+SQ+DS MQEK      G  PSSPL+F++IDRN DY G
Sbjct: 1217 INGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNVDYRG 1276

BLAST of HG10022286 vs. ExPASy TrEMBL
Match: A0A6J1C6H8 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111008850 PE=3 SV=1)

HSP 1 Score: 2229.5 bits (5776), Expect = 0.0e+00
Identity = 1113/1266 (87.91%), Postives = 1168/1266 (92.26%), Query Frame = 0

Query: 2    TNQLRSSVNRRRDFRDNNYKRKKQSIIQS--AEEDIESKFGFNLFSEGDKRLGWLLTFTS 61
            T  + +S +RRR+  D N+K KKQ I++S  AEE+IESKFGF LFSEGDKRLGWLLTF  
Sbjct: 17   TCNITNSESRRRNLTD-NHKSKKQRIVESATAEEEIESKFGFGLFSEGDKRLGWLLTFAP 76

Query: 62   SSCKDEDTEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQ 121
            SS KD+DT KVYSCIDLYFVTQDGSSFKSKY FRPYFYAATK+KME DVEAYL+RRY+G+
Sbjct: 77   SSRKDQDTGKVYSCIDLYFVTQDGSSFKSKYNFRPYFYAATKDKMETDVEAYLKRRYEGR 136

Query: 122  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAY 181
            IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVK+EL HVVESN EKSDAAEAY
Sbjct: 137  IADIEILEKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKNELFHVVESNWEKSDAAEAY 196

Query: 182  DSILFGKREQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRK 241
            DSILFGKREQ+ QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRK
Sbjct: 197  DSILFGKREQKPQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRK 256

Query: 242  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 301
            DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE
Sbjct: 257  DLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLE 316

Query: 302  YTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGL 361
            YTPKPEY+GFF+V NVKNELELLKLWF+HMREVKPGIYVTYNGDYFDWPFLESRAA+HGL
Sbjct: 317  YTPKPEYEGFFEVTNVKNELELLKLWFSHMREVKPGIYVTYNGDYFDWPFLESRAAFHGL 376

Query: 362  KMSDEVGFQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 421
            KMS+EVG QCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN
Sbjct: 377  KMSNEVGSQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVN 436

Query: 422  PEDMVRFAKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTL 481
            PEDMVRFAKEKPQMMASYSVSDAV+TYYLY+TYVHPFIFSLATIIPM+PDEVLRKGSGTL
Sbjct: 437  PEDMVRFAKEKPQMMASYSVSDAVSTYYLYITYVHPFIFSLATIIPMTPDEVLRKGSGTL 496

Query: 482  CEMLLMVQAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFR 541
            CEMLLMVQAY+A+VVCPNKHQSDPEKF+NNRLLESETYIGGHVECLESGVFRSD+PTSFR
Sbjct: 497  CEMLLMVQAYKADVVCPNKHQSDPEKFYNNRLLESETYIGGHVECLESGVFRSDIPTSFR 556

Query: 542  LDSSAYDQLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------- 601
            LDSSAYDQLI+NLDRDLQYAIQVEGKMD E+VSNYS+VK AI EK               
Sbjct: 557  LDSSAYDQLIDNLDRDLQYAIQVEGKMDXEMVSNYSNVKNAIMEKLIRLRDGPVREECPL 616

Query: 602  --------------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRS 661
                                PPS+VTDEVCTACDFN P KTCLR+LEWVW GEVF AK+S
Sbjct: 617  IYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNHPEKTCLRKLEWVWRGEVFMAKKS 676

Query: 662  DYYHLKRQIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEV 721
            DYYHLKRQIESEFVDSG +RLSK FLEL KLEQQAKLK+RLKKYCQKAYKRVLDKPVTEV
Sbjct: 677  DYYHLKRQIESEFVDSGDIRLSKSFLELQKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEV 736

Query: 722  RKAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLY 781
            R+AGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAK SGNSIKIQEAQDM+VLY
Sbjct: 737  REAGICMRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKESGNSIKIQEAQDMLVLY 796

Query: 782  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 841
            DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT
Sbjct: 797  DSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDT 856

Query: 842  DGIWCALPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEM 901
            DGIWCALPGSFPENFTFKTK+SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+KIY+ 
Sbjct: 857  DGIWCALPGSFPENFTFKTKDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKIYKT 916

Query: 902  RSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 961
             SECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK
Sbjct: 917  HSECSIEFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIK 976

Query: 962  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1021
            VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL
Sbjct: 977  VFQAELFDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSL 1036

Query: 1022 ADYGEQKSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDP 1081
            ADYGEQKSCAVTTA+RLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD 
Sbjct: 1037 ADYGEQKSCAVTTARRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDA 1096

Query: 1082 EIMKFYVRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPD 1141
            EIMKFYVRKWCKISSE GIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPD
Sbjct: 1097 EIMKFYVRKWCKISSEAGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPD 1156

Query: 1142 WLHKKVREKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSS 1201
            WLHKKVREKDDKFRQQKIV+MFASS G     N G S SK+VMN +NS+D+EDFQKKTSS
Sbjct: 1157 WLHKKVREKDDKFRQQKIVDMFASSNGAKFSKNSGFSCSKHVMNEENSEDLEDFQKKTSS 1216

Query: 1202 SSGPKPVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHG 1231
             +GPKPVVR YDVR MKHSV R+SQ+DS MQEK      G  PSSPL+F++IDRN DY G
Sbjct: 1217 INGPKPVVRYYDVRPMKHSVRRNSQIDSSMQEKINA--GGRGPSSPLYFENIDRNVDYRG 1276

BLAST of HG10022286 vs. ExPASy TrEMBL
Match: A0A6J1DHX7 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111020656 PE=3 SV=1)

HSP 1 Score: 2214.5 bits (5737), Expect = 0.0e+00
Identity = 1106/1255 (88.13%), Postives = 1155/1255 (92.03%), Query Frame = 0

Query: 12   RRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVY 71
            RR  R +N + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF  SS +D+DT KV+
Sbjct: 6    RRRGRGDNTRLKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSWEDQDTGKVF 65

Query: 72   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDL 131
            SCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+GQIADIEILEKEDL
Sbjct: 66   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGQIADIEILEKEDL 125

Query: 132  DLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRL 191
            DLKNHLSGL KAYLKLSFDTVQELM+VKSELLHVVESN  KSDAAEAYDSILFGKREQ+ 
Sbjct: 126  DLKNHLSGLCKAYLKLSFDTVQELMHVKSELLHVVESNWAKSDAAEAYDSILFGKREQKP 185

Query: 192  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICA 251
            QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+ LEKRKDLLQRAEV +CA
Sbjct: 186  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVMLEKRKDLLQRAEVHVCA 245

Query: 252  FDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFK 311
            FDIETTKLPLKFPDAEYDMIMMISYM+DGQGYLIINRECVGEDIENLEYTPKPEY+GFF+
Sbjct: 246  FDIETTKLPLKFPDAEYDMIMMISYMIDGQGYLIINRECVGEDIENLEYTPKPEYEGFFR 305

Query: 312  VNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDK 371
            V NVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHG KMSDEVGFQCDK
Sbjct: 306  VTNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSDEVGFQCDK 365

Query: 372  NQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKP 431
            NQGECRSKFACHLDCFAWVKRDSYLPQGS GLKAVTKAKLGYDPLEVNPEDMVR AKEKP
Sbjct: 366  NQGECRSKFACHLDCFAWVKRDSYLPQGSQGLKAVTKAKLGYDPLEVNPEDMVRSAKEKP 425

Query: 432  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEA 491
            QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAY+A
Sbjct: 426  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYKA 485

Query: 492  NVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINN 551
            NVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYDQLI N
Sbjct: 486  NVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYDQLIYN 545

Query: 552  LDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------------------- 611
            LDRDLQYAIQVEGKMDLELV NY+DVK AI EK                           
Sbjct: 546  LDRDLQYAIQVEGKMDLELVLNYNDVKNAIMEKLIRLRDEPVREEGPLIYHLDVAAMYPN 605

Query: 612  --------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESE 671
                    PPS+VTDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+QIESE
Sbjct: 606  IILTNRLQPPSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKKQIESE 665

Query: 672  FVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSF 731
            FVDSG VRLSK FLELPKLEQQ KLKERLKKYCQKAYKRVLDKPVTEVR+AGICMRENSF
Sbjct: 666  FVDSGNVRLSKSFLELPKLEQQTKLKERLKKYCQKAYKRVLDKPVTEVREAGICMRENSF 725

Query: 732  YIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 791
            YIDTVRSFRDRRYEYKGLNKVWKG+LSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN
Sbjct: 726  YIDTVRSFRDRRYEYKGLNKVWKGRLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 785

Query: 792  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFP 851
            SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNA LLIEKIGKPLELDTDGIWCALPGSFP
Sbjct: 786  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNACLLIEKIGKPLELDTDGIWCALPGSFP 845

Query: 852  ENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDG 911
            ENFTFKTK+ KKLTISYPCVMLNVDVARNNTNDQYQTL+DPVRKIY+  SECSIEFEVDG
Sbjct: 846  ENFTFKTKDLKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVRKIYKTHSECSIEFEVDG 905

Query: 912  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 971
            PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH
Sbjct: 906  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 965

Query: 972  GSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1031
            GSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT
Sbjct: 966  GSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1025

Query: 1032 TAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCK 1091
            TA+RLADFLGD MVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYVRKWCK
Sbjct: 1026 TARRLADFLGDAMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYVRKWCK 1085

Query: 1092 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDK 1151
            ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVREKDDK
Sbjct: 1086 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKDDK 1145

Query: 1152 FRQQKIVNMFAS-SRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKPVVRSY 1211
            FRQQK+ +MFAS +RGKY   N     SK+VMN +NS+D+EDFQKK SS++GPKPVVR Y
Sbjct: 1146 FRQQKVADMFASLNRGKYSGKNSDDGCSKHVMNEENSEDLEDFQKKASSTNGPKPVVRCY 1205

Query: 1212 DVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            DVR MKH   RDS+VDS+ Q  F G   G +PSSPLH +++D+N DYHGWLEMKK
Sbjct: 1206 DVRGMKH--PRDSEVDSVRQVNFNG--GGCEPSSPLHVENVDKNVDYHGWLEMKK 1256

BLAST of HG10022286 vs. ExPASy TrEMBL
Match: A0A6J1DJK0 (DNA polymerase epsilon catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111020656 PE=3 SV=1)

HSP 1 Score: 2214.5 bits (5737), Expect = 0.0e+00
Identity = 1106/1255 (88.13%), Postives = 1155/1255 (92.03%), Query Frame = 0

Query: 12   RRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVY 71
            RR  R +N + KKQ II+SAEE+IESK GF+LFSEGDKRLGWLLTF  SS +D+DT KV+
Sbjct: 6    RRRGRGDNTRLKKQRIIRSAEEEIESKLGFDLFSEGDKRLGWLLTFAPSSWEDQDTGKVF 65

Query: 72   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDL 131
            SCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+GQIADIEILEKEDL
Sbjct: 66   SCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGQIADIEILEKEDL 125

Query: 132  DLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRL 191
            DLKNHLSGL KAYLKLSFDTVQELM+VKSELLHVVESN  KSDAAEAYDSILFGKREQ+ 
Sbjct: 126  DLKNHLSGLCKAYLKLSFDTVQELMHVKSELLHVVESNWAKSDAAEAYDSILFGKREQKP 185

Query: 192  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICA 251
            QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+ LEKRKDLLQRAEV +CA
Sbjct: 186  QDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVMLEKRKDLLQRAEVHVCA 245

Query: 252  FDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFK 311
            FDIETTKLPLKFPDAEYDMIMMISYM+DGQGYLIINRECVGEDIENLEYTPKPEY+GFF+
Sbjct: 246  FDIETTKLPLKFPDAEYDMIMMISYMIDGQGYLIINRECVGEDIENLEYTPKPEYEGFFR 305

Query: 312  VNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDK 371
            V NVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHG KMSDEVGFQCDK
Sbjct: 306  VTNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGFKMSDEVGFQCDK 365

Query: 372  NQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKP 431
            NQGECRSKFACHLDCFAWVKRDSYLPQGS GLKAVTKAKLGYDPLEVNPEDMVR AKEKP
Sbjct: 366  NQGECRSKFACHLDCFAWVKRDSYLPQGSQGLKAVTKAKLGYDPLEVNPEDMVRSAKEKP 425

Query: 432  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEA 491
            QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAY+A
Sbjct: 426  QMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYKA 485

Query: 492  NVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINN 551
            NVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYDQLI N
Sbjct: 486  NVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYDQLIYN 545

Query: 552  LDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK--------------------------- 611
            LDRDLQYAIQVEGKMDLELV NY+DVK AI EK                           
Sbjct: 546  LDRDLQYAIQVEGKMDLELVLNYNDVKNAIMEKLIRLRDEPVREEGPLIYHLDVAAMYPN 605

Query: 612  --------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESE 671
                    PPS+VTDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+QIESE
Sbjct: 606  IILTNRLQPPSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKKQIESE 665

Query: 672  FVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSF 731
            FVDSG VRLSK FLELPKLEQQ KLKERLKKYCQKAYKRVLDKPVTEVR+AGICMRENSF
Sbjct: 666  FVDSGNVRLSKSFLELPKLEQQTKLKERLKKYCQKAYKRVLDKPVTEVREAGICMRENSF 725

Query: 732  YIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 791
            YIDTVRSFRDRRYEYKGLNKVWKG+LSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN
Sbjct: 726  YIDTVRSFRDRRYEYKGLNKVWKGRLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILN 785

Query: 792  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFP 851
            SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNA LLIEKIGKPLELDTDGIWCALPGSFP
Sbjct: 786  SFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNACLLIEKIGKPLELDTDGIWCALPGSFP 845

Query: 852  ENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDG 911
            ENFTFKTK+ KKLTISYPCVMLNVDVARNNTNDQYQTL+DPVRKIY+  SECSIEFEVDG
Sbjct: 846  ENFTFKTKDLKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVRKIYKTHSECSIEFEVDG 905

Query: 912  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 971
            PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH
Sbjct: 906  PYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLH 965

Query: 972  GSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1031
            GSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT
Sbjct: 966  GSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVT 1025

Query: 1032 TAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCK 1091
            TA+RLADFLGD MVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYVRKWCK
Sbjct: 1026 TARRLADFLGDAMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYVRKWCK 1085

Query: 1092 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDK 1151
            ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVREKDDK
Sbjct: 1086 ISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVREKDDK 1145

Query: 1152 FRQQKIVNMFAS-SRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKPVVRSY 1211
            FRQQK+ +MFAS +RGKY   N     SK+VMN +NS+D+EDFQKK SS++GPKPVVR Y
Sbjct: 1146 FRQQKVADMFASLNRGKYSGKNSDDGCSKHVMNEENSEDLEDFQKKASSTNGPKPVVRCY 1205

Query: 1212 DVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            DVR MKH   RDS+VDS+ Q  F G   G +PSSPLH +++D+N DYHGWLEMKK
Sbjct: 1206 DVRGMKH--PRDSEVDSVRQVNFNG--GGCEPSSPLHVENVDKNVDYHGWLEMKK 1256

BLAST of HG10022286 vs. ExPASy TrEMBL
Match: A0A5A7SVB8 (DNA polymerase epsilon catalytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00410 PE=3 SV=1)

HSP 1 Score: 2212.2 bits (5731), Expect = 0.0e+00
Identity = 1099/1260 (87.22%), Postives = 1160/1260 (92.06%), Query Frame = 0

Query: 7    SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 66
            S  +RRR   + + + KKQ +I+SAEE+IESK GF+LFSEGDKRLGWLLTF SSS +D+D
Sbjct: 2    SGESRRRGRAEKSSRSKKQRVIRSAEEEIESKLGFDLFSEGDKRLGWLLTFASSSLEDQD 61

Query: 67   TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 126
            T KVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATK+KME DVEAYLRRRY+G+IADIEIL
Sbjct: 62   TGKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKDKMETDVEAYLRRRYEGRIADIEIL 121

Query: 127  EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 186
            EKEDLDLKNHLSGL KAYLKLSFDTVQELMNVKSELLHVVE+N  KSDAAEAYDSILFGK
Sbjct: 122  EKEDLDLKNHLSGLRKAYLKLSFDTVQELMNVKSELLHVVENNWAKSDAAEAYDSILFGK 181

Query: 187  REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 246
            REQ+ QDFLECI+DLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTG+TLEKRKDLLQRAE
Sbjct: 182  REQKPQDFLECIIDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGVTLEKRKDLLQRAE 241

Query: 247  VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 306
            V ICAFDIETTKLPLKFPDAEYDMIMMISYMVDG GYLIINRECVGEDIENLEYTPKPEY
Sbjct: 242  VHICAFDIETTKLPLKFPDAEYDMIMMISYMVDGNGYLIINRECVGEDIENLEYTPKPEY 301

Query: 307  QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 366
            +GFFKV NVKNELELLKLWF+HMREVKPGIYVTYNGD+FDWPF+ESRAAYHG KMSDEVG
Sbjct: 302  EGFFKVTNVKNELELLKLWFSHMREVKPGIYVTYNGDFFDWPFIESRAAYHGFKMSDEVG 361

Query: 367  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 426
            FQCDKNQGECRSKFACHLDCF+WVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF
Sbjct: 362  FQCDKNQGECRSKFACHLDCFSWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 421

Query: 427  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 486
            AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV
Sbjct: 422  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 481

Query: 487  QAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 546
            QAY+ANVVCPNKHQSDPEKF++NRLLESETYIGGHVECLESGVFRSDLPTSFRLD SAYD
Sbjct: 482  QAYKANVVCPNKHQSDPEKFYSNRLLESETYIGGHVECLESGVFRSDLPTSFRLDPSAYD 541

Query: 547  QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK---------------------- 606
             LINNLDRDLQYAIQVEGKMDLE VSNYSDVK AI EK                      
Sbjct: 542  LLINNLDRDLQYAIQVEGKMDLESVSNYSDVKNAIMEKLIRLRDEPVREEGPLIYHLDVA 601

Query: 607  -------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKR 666
                         P S+VTDEVCTACDFNRPGKTCLR+LEWVW GEVF AK+SDYYHLK+
Sbjct: 602  AMYPNIILTNRLQPSSIVTDEVCTACDFNRPGKTCLRKLEWVWRGEVFMAKKSDYYHLKK 661

Query: 667  QIESEFVDSGTVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICM 726
            QIESEFVDSG VRLSK FLELPKLEQQAKLK+RLKKYCQKAYKRVLDKPVTEVR+AGICM
Sbjct: 662  QIESEFVDSGNVRLSKSFLELPKLEQQAKLKDRLKKYCQKAYKRVLDKPVTEVREAGICM 721

Query: 727  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAH 786
            RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAK+SGNSIKIQEAQDMVVLYDSLQLAH
Sbjct: 722  RENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKSSGNSIKIQEAQDMVVLYDSLQLAH 781

Query: 787  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 846
            KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL
Sbjct: 782  KCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCAL 841

Query: 847  PGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIE 906
            PGSFPENFTFKT++SKKLTISYPCVMLNVDVARNNTNDQYQTL+DPV+K Y+  SECSIE
Sbjct: 842  PGSFPENFTFKTRDSKKLTISYPCVMLNVDVARNNTNDQYQTLIDPVKKTYKTHSECSIE 901

Query: 907  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 966
            FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF
Sbjct: 902  FEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELF 961

Query: 967  DKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQK 1026
            DKFLHGSTLEECYSVVASVANRWLDLLD+QGKDIADSELLDYISESSTMSKSL DYGEQK
Sbjct: 962  DKFLHGSTLEECYSVVASVANRWLDLLDNQGKDIADSELLDYISESSTMSKSLVDYGEQK 1021

Query: 1027 SCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYV 1086
            SCAVTTA+RLADFLG+TMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETD EIMKFYV
Sbjct: 1022 SCAVTTARRLADFLGETMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDAEIMKFYV 1081

Query: 1087 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVR 1146
            RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKV+NPVPRVVHPDWLHKKVR
Sbjct: 1082 RKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVANPVPRVVHPDWLHKKVR 1141

Query: 1147 EKDDKFRQQKIVNMFA-SSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSSSGPKP 1206
            EKDDKFRQQK+ +MFA SSR KY + N   S SKN M+  NS+D+EDFQKKTSS++GPKP
Sbjct: 1142 EKDDKFRQQKVADMFASSSRVKYSKKNSDVSCSKNAMDEDNSEDLEDFQKKTSSTNGPKP 1201

Query: 1207 VVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
            VVR YDV+  KHS+T D +++S MQ+ F   + G +PSSPL  ++ID+N DY GWLE+KK
Sbjct: 1202 VVRCYDVKGKKHSLTSDVEINSEMQDNFN--DGGFEPSSPLDVENIDKNVDYSGWLEIKK 1259

BLAST of HG10022286 vs. TAIR 10
Match: AT1G08260.1 (DNA polymerase epsilon catalytic subunit )

HSP 1 Score: 1968.7 bits (5099), Expect = 0.0e+00
Identity = 972/1261 (77.08%), Postives = 1094/1261 (86.76%), Query Frame = 0

Query: 7    SSVNRRRDFRDNNYKRKKQSIIQSAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDED 66
            S  NRRRD +D  +  KK  ++ +AE+++ESK GF LFSEG+ RLGWLLTF+SSS +D D
Sbjct: 2    SGDNRRRDRKDTRWS-KKPKVVNTAEDELESKLGFGLFSEGETRLGWLLTFSSSSWEDRD 61

Query: 67   TEKVYSCIDLYFVTQDGSSFKSKYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEIL 126
            T KVYSC+DLYFVTQDG SFK+KYKFRPYFYAATK+KME ++EAYLRRRY+ Q+ADIEI+
Sbjct: 62   TGKVYSCVDLYFVTQDGFSFKTKYKFRPYFYAATKDKMELELEAYLRRRYERQVADIEIV 121

Query: 127  EKEDLDLKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGK 186
            EKEDLDLKNHLSGL K YLK+SFDTVQ+LM VK +LLH+VE N+ K DA EAY+SIL GK
Sbjct: 122  EKEDLDLKNHLSGLQKKYLKISFDTVQQLMEVKRDLLHIVERNQAKFDALEAYESILAGK 181

Query: 187  REQRLQDFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAE 246
            REQR QD L+ IVDLREYDVPYHVRFAID+D+R GQWY+VS+SST + LEKR DLLQRAE
Sbjct: 182  REQRPQDCLDSIVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAE 241

Query: 247  VRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEY 306
            VR+CAFDIETTKLPLKFPDAEYD IMMISYMVDGQG+LIINRECVGED+E+LEYTPKPE+
Sbjct: 242  VRVCAFDIETTKLPLKFPDAEYDQIMMISYMVDGQGFLIINRECVGEDVEDLEYTPKPEF 301

Query: 307  QGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVG 366
            +G+FKV NVKNE+ELL+ WF HM+E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+G
Sbjct: 302  EGYFKVTNVKNEVELLQRWFYHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELG 361

Query: 367  FQCDKNQGECRSKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 426
            F+CD+NQGECR+KFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF
Sbjct: 362  FRCDQNQGECRAKFACHLDCFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRF 421

Query: 427  AKEKPQMMASYSVSDAVATYYLYMTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMV 486
            A EKPQ MASYSVSDAVATYYLYMTYV+PFIFSLATIIPM PDEVLRKGSGTLCEMLLMV
Sbjct: 422  AMEKPQTMASYSVSDAVATYYLYMTYVNPFIFSLATIIPMVPDEVLRKGSGTLCEMLLMV 481

Query: 487  QAYEANVVCPNKHQSDPEKFHNNRLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYD 546
            +AY+ANVVCPNK+Q+DPEKF+ N+LLESETYIGGHVECLESGVFRSD+PTSF+LDSSAY 
Sbjct: 482  EAYKANVVCPNKNQADPEKFYQNQLLESETYIGGHVECLESGVFRSDIPTSFKLDSSAYQ 541

Query: 547  QLINNLDRDLQYAIQVEGKMDLELVSNYSDVKTAITEK---------------------- 606
            QLI+NL RDL+YAI VEGKM ++ +SNY +VK  I EK                      
Sbjct: 542  QLIDNLGRDLEYAITVEGKMRMDSISNYDEVKDEIKEKLEKLRDDPIREEGPLIYHLDVA 601

Query: 607  -------------PPSVVTDEVCTACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKR 666
                         PPS+VTDE+CTACDFNRPGKTCLR+LEWVW G  F  K+SDYYHLK+
Sbjct: 602  AMYPNIILTNRLQPPSIVTDEICTACDFNRPGKTCLRKLEWVWRGVTFMGKKSDYYHLKK 661

Query: 667  QIESEFVDSG-TVRLSKQFLELPKLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGIC 726
            QIESEFVD+G  +  SK FL+LPK++QQ+KLKERLKKYCQKAYKRVLDKP+TEVR+AGIC
Sbjct: 662  QIESEFVDAGANIMSSKSFLDLPKVDQQSKLKERLKKYCQKAYKRVLDKPITEVREAGIC 721

Query: 727  MRENSFYIDTVRSFRDRRYEYKGLNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLA 786
            MREN FY+DTVRSFRDRRYEYK LNKVWKGKLSEAKASGNSIKIQEAQDMVV+YDSLQLA
Sbjct: 722  MRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNSIKIQEAQDMVVVYDSLQLA 781

Query: 787  HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCA 846
            HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWC 
Sbjct: 782  HKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCC 841

Query: 847  LPGSFPENFTFKTKESKKLTISYPCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSI 906
            LPGSFPENFTFKT + KKLTISYPCVMLNVDVA+NNTNDQYQTLVDPVRK Y+  SECSI
Sbjct: 842  LPGSFPENFTFKTIDMKKLTISYPCVMLNVDVAKNNTNDQYQTLVDPVRKTYKSHSECSI 901

Query: 907  EFEVDGPYKAMILPASKEEGILIKKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAEL 966
            EFEVDGPYKAMI+PASKEEGILIKKRYAVFN DGTLAELKGFEIKRRGELKLIKVFQAEL
Sbjct: 902  EFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTLAELKGFEIKRRGELKLIKVFQAEL 961

Query: 967  FDKFLHGSTLEECYSVVASVANRWLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQ 1026
            FDKFLHGSTLEECYS VA+VA+RWLDLLD+QGKDIADSELLDYISESSTMSKSLADYGEQ
Sbjct: 962  FDKFLHGSTLEECYSAVAAVADRWLDLLDNQGKDIADSELLDYISESSTMSKSLADYGEQ 1021

Query: 1027 KSCAVTTAKRLADFLGDTMVRDKGLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFY 1086
            KSCAVTTAKRLA+FLG TMV+DKGLRCQYIVACEPKGTPVSERAVPVAIF T+PE+MKF+
Sbjct: 1022 KSCAVTTAKRLAEFLGVTMVKDKGLRCQYIVACEPKGTPVSERAVPVAIFTTNPEVMKFH 1081

Query: 1087 VRKWCKISSEVGIRSIIDWSYYKQRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKV 1146
            +RKWCK SS+VGIR IIDWSYYKQRLSSAIQK+ITIPAAMQKV+NPVPRV+HPDWLHKKV
Sbjct: 1082 LRKWCKTSSDVGIRLIIDWSYYKQRLSSAIQKVITIPAAMQKVANPVPRVLHPDWLHKKV 1141

Query: 1147 REKDDKFRQQKIVNMFASSRGKYLENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPK 1206
            REKDDKFRQ+K+V+MF+S+      N D    +   +   N +DIEDF K+   S  GPK
Sbjct: 1142 REKDDKFRQRKLVDMFSSA------NKDVVLDTDLPVTKDNVEDIEDFCKENRPSVKGPK 1201

Query: 1207 PVVRSYDVRAMKHSVTRDSQVDSMMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMK 1231
            P+ RSY+V              +  Q + +  ES       + F++ID++ +Y GWLE+K
Sbjct: 1202 PIARSYEV--------------NKKQSECEQQESWDTEFHDISFQNIDKSVNYQGWLELK 1241

BLAST of HG10022286 vs. TAIR 10
Match: AT2G27120.1 (DNA polymerase epsilon catalytic subunit )

HSP 1 Score: 1859.3 bits (4815), Expect = 0.0e+00
Identity = 924/1238 (74.64%), Postives = 1049/1238 (84.73%), Query Frame = 0

Query: 30   SAEEDIESKFGFNLFSEGDKRLGWLLTFTSSSCKDEDTEKVYSCIDLYFVTQDGSSFKSK 89
            SA +++E+K GF LFS+G+ RLGWLLTF SSS +D DT K +SC+DL+FVTQDGSSFK+K
Sbjct: 16   SAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTK 75

Query: 90   YKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLDLKNHLSGLHKAYLKLSF 149
            YKFRPY YAATK+ ME +VEAYLRRRY+ Q+ADI+I+ KEDL LKNHLSGL K YLK+SF
Sbjct: 76   YKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSF 135

Query: 150  DTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQDFLECIVDLREYDVPYH 209
            DTVQ+L+ VK +LLH+VE N  K +A EAY+SIL GKREQR QD L+ +VDLREYDVPYH
Sbjct: 136  DTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYH 195

Query: 210  VRFAIDHDLRCGQWYDVSVSSTGITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYD 269
            VRFAID+D+R GQWY+VS+SST + LEKR DLLQRAEVR+CAFDIET KLPLKFPDAEYD
Sbjct: 196  VRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYD 255

Query: 270  MIMMISYMVDGQGYLIINRECVGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHM 329
             IMMISYMVDGQG+LI NRECVG+DIE+LEYTPKPE++G+FKV NV NE+ELL+ WF+HM
Sbjct: 256  QIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHM 315

Query: 330  REVKPGIYVTYNGDYFDWPFLESRAAYHGLKMSDEVGFQCDKNQGECRSKFACHLDCFAW 389
            +E+KPGIYVTYNGD+FDWPF+E RA++HG+KM++E+GF+CD+NQGECR+KF CHLDCF+W
Sbjct: 316  QELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSW 375

Query: 390  VKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPEDMVRFAKEKPQMMASYSVSDAVATYYLY 449
            VKRDSYLPQGS GLKAVTK KLGYDPLEVNPEDMVRFA EKPQ MASYSVSDAVATYYLY
Sbjct: 376  VKRDSYLPQGSQGLKAVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLY 435

Query: 450  MTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQSDPEKFHNN 509
            MTYVHPF+FSLATIIPM PDEVLRKGSGTLCEMLLMV+AY+ANVVCPNK+Q+DPEKF+  
Sbjct: 436  MTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQG 495

Query: 510  RLLESETYIGGHVECLESGVFRSDLPTSFRLDSSAYDQLINNLDRDLQYAIQVEGKMDLE 569
            +LLESETYIGGHVECL+SGVFRSD+PTSF+LD+SAY QLI+NL RDL+YAI VEGKM ++
Sbjct: 496  KLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEYAITVEGKMRMD 555

Query: 570  LVSNYSDVKTAITEK-----------------------------------PPSVVTDEVC 629
             VSN+ +VK  I EK                                   PPS+VTDEVC
Sbjct: 556  SVSNFDEVKEVIREKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEVC 615

Query: 630  TACDFNRPGKTCLRELEWVWHGEVFEAKRSDYYHLKRQIESEFVDSG-TVRLSKQFLELP 689
            TACDFN P KTCLR+LEWVW G  F+  +S+YYHLK+QIESE VD+G  ++ SK FL+LP
Sbjct: 616  TACDFNGPEKTCLRKLEWVWRGVTFKGNKSEYYHLKKQIESESVDAGANMQSSKPFLDLP 675

Query: 690  KLEQQAKLKERLKKYCQKAYKRVLDKPVTEVRKAGICMRENSFYIDTVRSFRDRRYEYKG 749
            K+EQQ+KLKERLKKYCQKAY RVLDKP+TEVR+AGICMREN FY+DTVRSFRDRRYEYK 
Sbjct: 676  KVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKT 735

Query: 750  LNKVWKGKLSEAKASGNSIKIQEAQDMVVLYDSLQLAHKCILNSFYGYVMRKGARWYSME 809
            LNKVWKGKLSEAKASGN IKIQEA DMVV+YDSLQLAHKCILNSFYGYVMRKGARWYSME
Sbjct: 736  LNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSME 795

Query: 810  MAGVVTYTGAKIIQNARLLIEKIGKPLELDTDGIWCALPGSFPENFTFKTKESKKLTISY 869
            MAGVVTYTGAKIIQNARLLIE+IGKPLELDTDGIWCALPGSFPENFTFKT + KK TISY
Sbjct: 796  MAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFTISY 855

Query: 870  PCVMLNVDVARNNTNDQYQTLVDPVRKIYEMRSECSIEFEVDGPYKAMILPASKEEGILI 929
            PCV+LNVDVA+NN+NDQYQTLVDPVRK Y  RSECSIEFEVDGPYKAMI+PASKEEGILI
Sbjct: 856  PCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEGILI 915

Query: 930  KKRYAVFNDDGTLAELKGFEIKRRGELKLIKVFQAELFDKFLHGSTLEECYSVVASVANR 989
            KKRYAVFN DGT+AELKGFE+KRRGELKLIKVFQAELFDKFLHGSTLEECYS VA+VANR
Sbjct: 916  KKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVANR 975

Query: 990  WLDLLDSQGKDIADSELLDYISESSTMSKSLADYGEQKSCAVTTAKRLADFLGDTMVRDK 1049
            WLDLL+ QGKDIADSELLDYISESSTMSKSLADYG+QKSCAVTTAKRLADFLGDTMV+DK
Sbjct: 976  WLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTMVKDK 1035

Query: 1050 GLRCQYIVACEPKGTPVSERAVPVAIFETDPEIMKFYVRKWCKISSEVGIRSIIDWSYYK 1109
            GLRCQYIVA EP+GTPVSERAVPVAIF+TD    KFY++KWCKISS  GIRSIIDW YYK
Sbjct: 1036 GLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDWMYYK 1095

Query: 1110 QRLSSAIQKIITIPAAMQKVSNPVPRVVHPDWLHKKVREKDDKFRQQKIVNMFASSRGKY 1169
            QRL SAIQK+ITIPAAMQKV+NPV RV HP WL KKV    DKFRQ KIV+MF+S+    
Sbjct: 1096 QRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKV---CDKFRQGKIVDMFSSA---- 1155

Query: 1170 LENNDGASSSKNVMNGKNSDDIEDFQKKTSSS-SGPKPVVRSYDVRAMKHSVTRDSQVDS 1229
              N D +++  NV+      DIE+F K+   S  GPKPV RS++V    HS         
Sbjct: 1156 --NKDHSTTQDNVV-----ADIEEFCKENRPSVKGPKPVARSFEV-DRNHS--------- 1215

Query: 1230 MMQEKFKGAESGSDPSSPLHFKDIDRNEDYHGWLEMKK 1231
                + K  ES       +  +++D+N DY GWLE++K
Sbjct: 1216 ----EGKQQESWDPEFHDISLQNVDKNVDYQGWLELEK 1225

BLAST of HG10022286 vs. TAIR 10
Match: AT5G63960.1 (DNA binding;nucleotide binding;nucleic acid binding;DNA-directed DNA polymerases;DNA-directed DNA polymerases )

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-09
Identity = 107/498 (21.49%), Postives = 190/498 (38.15%), Query Frame = 0

Query: 74  IDLYFVTQDGSSFKS-KYKFRPYFYAATKNKMENDVEAYLRRRYKGQIADIEILEKEDLD 133
           I ++ VT++G+S     + F PYFY A    M  D  +   +  +G++ +     K    
Sbjct: 124 IRMFGVTREGNSVCCFVHGFEPYFYIACPPGMGPDDISNFHQSLEGRMRESNKNAKVPKF 183

Query: 134 LKNHLSGLHKAYLKLSFDTVQELMNVKSELLHVVESNREKSDAAEAYDSILFGKREQRLQ 193
           +K       ++ +       Q  + +   L  +V S R   D     D    G   +  Q
Sbjct: 184 VKRIEMVQKRSIMYYQQQKSQTFLKITVALPTMVASCRGILDRGLQID----GLGMKSFQ 243

Query: 194 DFLECIVDLREYDVPYHVRFAIDHDLRCGQWYDVSVS--------------------STG 253
            +        E ++ + +RF +D D+  G W +V                       S  
Sbjct: 244 TY--------ESNILFVLRFMVDCDIVGGNWIEVPTGKYKKNARTLSYCQLEFHCLYSDL 303

Query: 254 ITLEKRKDLLQRAEVRICAFDIETTKLPLKFPDAEYDMIMMISYMVDGQG-------YLI 313
           I+     +  + A  R+ +FDIE       FP+A++D ++ I+ +V  QG        ++
Sbjct: 304 ISHAAEGEYSKMAPFRVLSFDIECAGRKGHFPEAKHDPVIQIANLVTLQGEDHPFVRNVM 363

Query: 314 INREC---VGEDIENLEYTPKPEYQGFFKVNNVKNELELLKLWFAHMREVKPGIYVTYNG 373
             + C   VG D+ + E                  E E+L  W   +R+V P I + YN 
Sbjct: 364 TLKSCAPIVGVDVMSFE-----------------TEREVLLAWRDLIRDVDPDIIIGYNI 423

Query: 374 DYFDWPFLESRAAYHGLKMSDEVG------------FQCDKNQGECRSKFAC-----HLD 433
             FD P+L  RAA  G++    +G                + QG   SK          D
Sbjct: 424 CKFDLPYLIERAATLGIEEFPLLGRVKNSRVRVRDSTFSSRQQGIRESKETTIEGRFQFD 483

Query: 434 CFAWVKRDSYLPQGSHGLKAVTKAKLGYDPLEVNPE---DMVRFAKEKPQMMASYSVSDA 493
               + RD  L   S+ L +V+   L     +V+     D+     E  + +A Y + DA
Sbjct: 484 LIQAIHRDHKL--SSYSLNSVSAHFLSEQKEDVHHSIITDLQNGNAETRRRLAVYCLKDA 543

Query: 494 VATYYLY--MTYVHPFIFSLATIIPMSPDEVLRKGSGTLCEMLLMVQAYEANVVCPNKHQ 517
                L   + +++ ++  +A +  +    +L +G        L+ +  + N+V PN  Q
Sbjct: 544 YLPQRLLDKLMFIYNYV-EMARVTGVPISFLLARGQSIKVLSQLLRKGKQKNLVLPNAKQ 589