HG10022188 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022188 vs. NCBI nr
Match: KAG6602402.1 (Cytochrome P450 724B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 647/876 (73.86%), Postives = 715/876 (81.62%), Query Frame = 0

Query: 6   ASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHS 65
           ASFSP + I L+L++A +I+C  L+KLF   + P+P+LPPGCMGFPFVGETLSFLKPHHS
Sbjct: 5   ASFSP-LTIALSLAMAAVILCQLLLKLFA-AATPSPSLPPGCMGFPFVGETLSFLKPHHS 64

Query: 66  NSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILG 125
           NSIGTFLQ HCSR+GKVFKSHLFG  AIVSCDRELN+FILQNDDKLFKVSYPKAMHGILG
Sbjct: 65  NSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 124

Query: 126 SNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELK 185
           SNSLIIAAG THRKLRNVVVSF SWCKS P+FLH +  LS+SLM SWRSQT++LFCKE+K
Sbjct: 125 SNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVK 184

Query: 186 MFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYI 245
           MFALSVMVKELLGI+ NESIGS+IL+EFETYMKGFVSLPLNL GTPY KAVKAR +LS I
Sbjct: 185 MFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLSGI 244

Query: 246 VKEMMREKRRRGLLEG------NGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 305
           VKEMMRE++++GL+ G      + E+FL VIMSNWKL +EEIVSVVLDILLGGYETTATL
Sbjct: 245 VKEMMRERKKKGLVVGVEKERDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATL 304

Query: 306 MGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNV 365
           MGLIVYFLAHSPP+VLAKLKEE + IRNGK  GE LNLEDYKQMEFTCNVI EAMRCGNV
Sbjct: 305 MGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCGNV 364

Query: 366 VKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRW-----TKKKS 425
           VKFLHREA+ DVKFKDI IPSGWKVHPVFSAIHLDPTLHP PQ F+P RW       KK 
Sbjct: 365 VKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDKEMSKKV 424

Query: 426 SPTPSRARQSFTVSARR----FF-----------------PSGGPSGENPWRRHLRRQQL 485
           +P     R    +   +    FF                 P   P  ++ +R  +R    
Sbjct: 425 TPFGGGPRLCPGIELAKLEIAFFLHHLVLNYRWKTSADECPLAYPYLQSLFRLFIRAAHC 484

Query: 486 QTHLDERQRLRTGGSTLECKMSK-ESFEVNLTTDCSPESSKKRAKIRKNRKKQLLMEGVD 545
           +        L    + +  +M K ESFEV LTTDCSPESSKK  K  K +KKQLL+EGVD
Sbjct: 485 RP-------LFLSLARVTARMPKEESFEVGLTTDCSPESSKK-LKGSKKKKKQLLVEGVD 544

Query: 546 VMEMKEVGSVQLGESNINSDSDKVKKKKKIKKSTQQCIEESSMKDEAGEYENRAETFVKE 605
           VME    GS +L ESNINS+   VK KKK KK T QC+ E+S++ EAGEYE +AE  VKE
Sbjct: 545 VMERNVEGSFELWESNINSE---VKNKKKRKKITIQCVVENSIQAEAGEYEIKAERSVKE 604

Query: 606 GSPSSNGGNEK--FFERRKIALEDAENERTGSIVRENSGEKIPESNNEKADQRNIKRKKR 665
           GSP S+GG+EK    E R+ ALE+ ENER  SIVRE SGEKI ESNN K DQRNIKRKKR
Sbjct: 605 GSPLSDGGSEKNEILEERQFALENDENERADSIVRETSGEKILESNNGKTDQRNIKRKKR 664

Query: 666 LLKEAGNSDMRGICYLSRVPPHMDPLRLRQILSQYGEIQRIYLAPEDAANQVQRKRAGGF 725
           LLKE  N+DMRGICYLSRVPPHMDPL+LRQILSQYGEIQRIYLAPEDAANQV+RKRAGGF
Sbjct: 665 LLKEVANADMRGICYLSRVPPHMDPLKLRQILSQYGEIQRIYLAPEDAANQVKRKRAGGF 724

Query: 726 RGQFFSEGWVEFTDKRVAKKVANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEET 785
           RGQFFSEGWVEFTDKRVAKKVANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEET
Sbjct: 725 RGQFFSEGWVEFTDKRVAKKVANMLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEET 784

Query: 786 AYKHAIREQKLALEISAAKRERDFYLAKVDKSRALNSIEERLKKKQKLQEDSEINSALAD 845
           AYKHAIREQK+ALEISAAKRERDFYL+KVDKSRAL SIEERLKKKQKL+EDSE+NS LAD
Sbjct: 785 AYKHAIREQKMALEISAAKRERDFYLSKVDKSRALKSIEERLKKKQKLREDSEMNSELAD 844

Query: 846 SQKLPKLIRSFPQTQPVAHSAVQNKQRLSTNVLAGV 847
           SQKLPKLIR+FPQTQPVA SAVQ+K +LSTN LAGV
Sbjct: 845 SQKLPKLIRNFPQTQPVADSAVQDKPKLSTNFLAGV 867

BLAST of HG10022188 vs. NCBI nr
Match: XP_038889747.1 (cytochrome P450 724B1 [Benincasa hispida])

HSP 1 Score: 757.7 bits (1955), Expect = 1.1e-214
Identity = 379/414 (91.55%), Postives = 393/414 (94.93%), Query Frame = 0

Query: 1   MEVIGASFSPMIIIVLALSLAILIICHSLIKLFVI-TSPPNPNLPPGCMGFPFVGETLSF 60
           MEVI  SFSPMIIIVLALS AILII HSL+KLFVI TS PNPNLPPGCMGFPFVGETLSF
Sbjct: 1   MEVISGSFSPMIIIVLALSFAILIISHSLLKLFVITTSTPNPNLPPGCMGFPFVGETLSF 60

Query: 61  LKPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKA 120
           LKPHHSNSIGTFLQHHCSRYGKVFKSHLFG PAIVSCDRELN+FILQNDDKLFKVSYPKA
Sbjct: 61  LKPHHSNSIGTFLQHHCSRYGKVFKSHLFGWPAIVSCDRELNIFILQNDDKLFKVSYPKA 120

Query: 121 MHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLL 180
           MHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRP+FLHSLH+LSISLM SWRSQTQLL
Sbjct: 121 MHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPTFLHSLHSLSISLMDSWRSQTQLL 180

Query: 181 FCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKAR 240
           FCK++K+FALSVMVKEL GI+  ESIGSKIL+EFETYMKGFVSLPL LPGTPYSKAVKAR
Sbjct: 181 FCKQVKLFALSVMVKELFGIEAKESIGSKILKEFETYMKGFVSLPLKLPGTPYSKAVKAR 240

Query: 241 GRLSYIVKEMMREKRRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 300
            RLSYIVKEMMRE+RRRGLLEGN EDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL
Sbjct: 241 ERLSYIVKEMMRERRRRGLLEGNDEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 300

Query: 301 MGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNV 360
           MGLI+YFL+HSPPNVLAKLKEE QAIR GKRNGECLNLEDYKQMEFTCNVI EAMRCGNV
Sbjct: 301 MGLIIYFLSHSPPNVLAKLKEEHQAIRFGKRNGECLNLEDYKQMEFTCNVIFEAMRCGNV 360

Query: 361 VKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           VKFLHREALTDVKFKDIVIP GWKVHPVFSAIHLDPT+HPNPQHF+P RWT  K
Sbjct: 361 VKFLHREALTDVKFKDIVIPCGWKVHPVFSAIHLDPTIHPNPQHFNPWRWTDDK 414

BLAST of HG10022188 vs. NCBI nr
Match: XP_011655382.1 (cytochrome P450 724B1 [Cucumis sativus] >KGN51340.1 hypothetical protein Csa_009440 [Cucumis sativus])

HSP 1 Score: 665.6 bits (1716), Expect = 5.7e-187
Identity = 334/416 (80.29%), Postives = 369/416 (88.70%), Query Frame = 0

Query: 1   MEVIGASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFL 60
           MEVIG + SPMIIIVLALS AI II H L+KLF++TS  NPNLPPG MG PFVGETLSFL
Sbjct: 1   MEVIGTNLSPMIIIVLALSFAISIIFHLLLKLFLVTSKKNPNLPPGSMGLPFVGETLSFL 60

Query: 61  KPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAM 120
            PHHSNSIGTFLQHH SRYGK+FKS LFG PAIVSCDRELN F+LQNDDKLFKVSYPKAM
Sbjct: 61  NPHHSNSIGTFLQHHFSRYGKIFKSRLFGRPAIVSCDRELNYFVLQNDDKLFKVSYPKAM 120

Query: 121 HGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLF 180
           H ILG+NSL+I+AG THRKLR+V+VSF + CK+RP+FLHSLHNLS+SL  SWRSQT + F
Sbjct: 121 HNILGTNSLLISAGDTHRKLRSVIVSFITRCKTRPNFLHSLHNLSVSLTDSWRSQTHVSF 180

Query: 181 CKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARG 240
            KE+KMFALS+MVKE+ GI+  E IG+KI EEFET+M GFVSLPLN PGTPY KAVKARG
Sbjct: 181 IKEMKMFALSLMVKEVFGIEAKELIGTKIFEEFETFMIGFVSLPLNFPGTPYFKAVKARG 240

Query: 241 RLSYIVKEMMREKRRRGLLEGNG--EDFLQVIMS-NWKLSDEEIVSVVLDILLGGYETTA 300
           RLS IVKEM+RE+R+RGL+ GN   +DFLQV+MS NWKLSDEEIVSVVLDI+LG YETTA
Sbjct: 241 RLSTIVKEMIRERRKRGLVGGNNNDDDFLQVLMSNNWKLSDEEIVSVVLDIMLGSYETTA 300

Query: 301 TLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCG 360
           TL+GLIVYFLAHSPPN+LAKLKEE QAIRNGKR GECLNLEDYK+MEFT NV  EAMRCG
Sbjct: 301 TLLGLIVYFLAHSPPNILAKLKEEHQAIRNGKRKGECLNLEDYKRMEFTFNVAYEAMRCG 360

Query: 361 NVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           NVVKFLHREA+TDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQ F+PSRW+  K
Sbjct: 361 NVVKFLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPSRWSDDK 416

BLAST of HG10022188 vs. NCBI nr
Match: XP_022133522.1 (cytochrome P450 724B1 [Momordica charantia])

HSP 1 Score: 659.8 bits (1701), Expect = 3.1e-185
Identity = 336/417 (80.58%), Postives = 368/417 (88.25%), Query Frame = 0

Query: 1   MEVI--GASFSPMIIIVLALSLAI-LIICHSLIKLFVITSPPN-PNLPPGCMGFPFVGET 60
           ME++   AS SP  I+V+ALSLA+  II HSL+KL VI +  N PNLPPG MGFPF+G+T
Sbjct: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60

Query: 61  LSFLKPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSY 120
           L  LKPH SNSIGTFL HHCSRYGKVFKSHLFG PAIVSCD+ELN+FILQNDDKLFKVSY
Sbjct: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120

Query: 121 PKAMHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQT 180
           PKAMHGILG+NSLIIAAG THRKLRNVVVSF  WCKSRPSFLH +HNL+ISLM SWRSQ 
Sbjct: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180

Query: 181 QLLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAV 240
           Q+ FCKE+KMFALS+MVKELLGI+  E+IGSKILEEFE YMKGFVSLP+NLPGT YS AV
Sbjct: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240

Query: 241 KARGRLSYIVKEMMREKRRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETT 300
           KAR RLS +VKEMMRE++RRG++ G  EDFLQVIMSNWKL+DEEIVSVVLDILLGGYETT
Sbjct: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300

Query: 301 ATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRC 360
           ATLMGLIVYFLAHSPPNVLAKLKEE +AIR  KRNGE LNLEDYKQMEFT NV+ EAMRC
Sbjct: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360

Query: 361 GNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           GNVVKFLHREA+ DVKFK+IVIPSGWKVHPVFSA+HLDP LHPNPQ F+P RWT  K
Sbjct: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDK 417

BLAST of HG10022188 vs. NCBI nr
Match: XP_022990088.1 (cytochrome P450 724B1 [Cucurbita maxima])

HSP 1 Score: 657.9 bits (1696), Expect = 1.2e-184
Identity = 330/414 (79.71%), Postives = 366/414 (88.41%), Query Frame = 0

Query: 6   ASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHS 65
           ASFSP + I L+L++A +I+C  L+KLF   + P+P+LPPGCMGFPFVGETLSFLKPHHS
Sbjct: 5   ASFSP-LSIALSLAMAAVILCQLLLKLFA-AATPSPSLPPGCMGFPFVGETLSFLKPHHS 64

Query: 66  NSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILG 125
           NSIGTFLQ+HCSR+G VFKSHLFG PAIVSCDRELN+FILQNDDKLFKVSYPKAMHGILG
Sbjct: 65  NSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 124

Query: 126 SNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELK 185
           SNSLIIAAG THRKLRNVVVSF SWCKS P+FLHS+  LS+SLM SWR QT++ FCKE+K
Sbjct: 125 SNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKEVK 184

Query: 186 MFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYI 245
           MFALSVMVKELLGI+ NES+G +IL+EFETYMKGFVSLPLNLPGTPY KAVKAR +LS I
Sbjct: 185 MFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGI 244

Query: 246 VKEMMREKRRRGLLEG------NGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 305
           VKEMM+E++++GL+ G      + E+FL VIMSNWKL +EEIVSVVLDILLGGYETTATL
Sbjct: 245 VKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATL 304

Query: 306 MGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNV 365
           MGLIVYFLAHSPPNVLAKLKEE +AIRNGK  GE LNLEDYKQMEFTCNVI EAMRCGNV
Sbjct: 305 MGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCGNV 364

Query: 366 VKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           VKFLHREA+ DVKFKDI IPSGWKVHPVFSAIHLDPTLHP PQ F+P RW   K
Sbjct: 365 VKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDK 416

BLAST of HG10022188 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 9.0e-111
Identity = 204/427 (47.78%), Postives = 293/427 (68.62%), Query Frame = 0

Query: 3   VIGASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKP 62
           ++G       +++L   L  L++ H L  L       NP  P G  G+P +GETL FL P
Sbjct: 1   MVGGELVLAALVILLALLLTLVLSHFLPLLL------NPKAPKGSFGWPLLGETLRFLSP 60

Query: 63  HHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHG 122
           H SN++G+FL+ HCSRYG+VFKSHLF  P IVSCD+ELN FILQN+++LF+ SYP+ +HG
Sbjct: 61  HASNTLGSFLEDHCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNEERLFQCSYPRPIHG 120

Query: 123 ILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQ----- 182
           ILG +S+++  G  H++LRN+ ++  +  K +PS+L  +  +++ ++ SW  +++     
Sbjct: 121 ILGKSSMLVVLGEDHKRLRNLALALVTSTKLKPSYLGDIEKIALHIVGSWHGKSKDKGMV 180

Query: 183 --LLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKA 242
             + FC+E + FA SV+VK++LG+   E + + ILE+F  +MKG +S PL +PGTPY+KA
Sbjct: 181 NVIAFCEEARKFAFSVIVKQVLGLSPEEPVTAMILEDFLAFMKGLISFPLYIPGTPYAKA 240

Query: 243 VKARGRLSYIVKEMMREKRRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYET 302
           V+AR R+S  VK ++ E+R  G    N  DFL V++S+ +LSDEE VS VLD LLGGYET
Sbjct: 241 VQARARISSTVKGIIEERRNAG--SSNKGDFLDVLLSSNELSDEEKVSFVLDSLLGGYET 300

Query: 303 TATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMR 362
           T+ L+ ++VYFL  S  + L  +K E + IR+ K   E L+ EDYK+ME+T +VI+EA+R
Sbjct: 301 TSLLISMVVYFLGQSAQD-LELVKREHEGIRSKKEKDEFLSSEDYKKMEYTQHVINEALR 360

Query: 363 CGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRW------ 417
           CGN+VKF+HR+AL DV++K+ +IPSGWKV PVFSA+HL+P LH N Q F P RW      
Sbjct: 361 CGNIVKFVHRKALKDVRYKEYLIPSGWKVLPVFSAVHLNPLLHGNAQQFQPCRWEGASQG 418

BLAST of HG10022188 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.5e-77
Identity = 155/401 (38.65%), Postives = 242/401 (60.35%), Query Frame = 0

Query: 42  NLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELN 101
           NLPPG  G+P VGET  +L+ H + S+G F++ H +RYGK+++S LFG   +VS D  LN
Sbjct: 53  NLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLN 112

Query: 102 VFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSL 161
            +ILQN+ +LF+ SYP+++ GILG  S+++  G  HR++R + ++F S  + R   L  +
Sbjct: 113 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEV 172

Query: 162 HNLSISLMASWRSQTQLLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFV 221
              ++ ++ +W   +      + K F  ++M K ++ +   E    ++  E+ T+MKG V
Sbjct: 173 ERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVV 232

Query: 222 SLPLNLPGTPYSKAVKARGRLSYIVKEMMREKRRRGLLEG---NGEDFLQVIMSNWKLSD 281
           S PLNLPGTPY KA+K+R  +  +++  M E+  +   E      +D L   +    LS 
Sbjct: 233 SAPLNLPGTPYWKALKSRAAILGVIERKMEERVEKLSKEDASVEQDDLLGWALKQSNLSK 292

Query: 282 EEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEPQAI-RNGKRNGEC-LN 341
           E+I+ ++L +L  G+ET++  + L ++FL    P  + +L+EE   I R  +  GEC L+
Sbjct: 293 EQILDLLLSLLFAGHETSSMALALAIFFL-EGCPKAVQELREEHLGIARRQRLRGECKLS 352

Query: 342 LEDYKQMEFTCNVISEAMRCGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPT 401
            EDYK+M FT  VI+E +R GNVV+FLHR+ + DV +K   IPSGWK+ PV +A+HLD +
Sbjct: 353 WEDYKEMVFTQCVINETLRLGNVVRFLHRKVIKDVHYKGYDIPSGWKILPVLAAVHLDSS 412

Query: 402 LHPNPQHFDPSRWTKKKSSPTPSRARQSFTVSARRFFPSGG 438
           L+ +PQ F+P RW   KSS +     QS +     F P GG
Sbjct: 413 LYEDPQRFNPWRW---KSSGSSGGLAQSSS-----FMPYGG 444

BLAST of HG10022188 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 1.9e-76
Identity = 166/455 (36.48%), Postives = 266/455 (58.46%), Query Frame = 0

Query: 10  PMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHSNSIG 69
           P++++   LSL +      LI L         NLPPG  G+PF+GET+ +LKP+ + ++G
Sbjct: 11  PLLLLPSLLSLLLF-----LILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLG 70

Query: 70  TFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILGSNSL 129
            F+Q H S+YGK+++S+LFG P IVS D  LN FILQN+ +LF+ SYP+++ GILG  S+
Sbjct: 71  DFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSM 130

Query: 130 IIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELKMFAL 189
           ++  G  HR +R++ ++F S  + R   L  +   ++ ++ SW+  +      E K F  
Sbjct: 131 LVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTF 190

Query: 190 SVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYIVKEM 249
           ++M K ++ +   E    ++ +E+ T+MKG VS PLNLPGT Y KA+++R  +   ++  
Sbjct: 191 NLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERK 250

Query: 250 MREKRRRGLLEGNGE-------------------------DFLQVIMSNWKLSDEEIVSV 309
           M E+R+  + E + E                         D L  ++ +  LS E+I+ +
Sbjct: 251 M-EERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDL 310

Query: 310 VLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRN-GEC-LNLEDYKQ 369
           +L +L  G+ET++  + L ++FL  + P  + +L+EE   I   K+  GE  LN +DYK+
Sbjct: 311 ILSLLFAGHETSSVAIALAIFFL-QACPKAVEELREEHLEIARAKKELGESELNWDDYKK 370

Query: 370 MEFTCNVISEAMRCGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQ 429
           M+FT  VI+E +R GNVV+FLHR+AL DV++K   IPSGWKV PV SA+HLD + +  P 
Sbjct: 371 MDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPN 430

Query: 430 HFDPSRWTKKKSSPTPSRARQSFTVSARRFFPSGG 438
            F+P RW +++++   S    SF+     + P GG
Sbjct: 431 LFNPWRW-QQQNNGASSSGSGSFSTWGNNYMPFGG 457

BLAST of HG10022188 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.6e-59
Identity = 142/412 (34.47%), Postives = 222/412 (53.88%), Query Frame = 0

Query: 21  AILIICHSLIKLFVI----TSPPNPNLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQHHC 80
           A L++  S+   F++    T      LPPG +G P +GET   +  + + +   F+    
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 81  SRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGHT 140
           +RYG VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 141 HRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELKMFALSVMVKEL 200
           H+++ ++ +SF +    +   +  +  L    + SW S  ++L  +E K     + VK+L
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQL 184

Query: 201 LGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLS---YIVKEMMREK 260
           +     E     + +E+   ++GF SLPL L  T Y KA++AR +++    +V    RE+
Sbjct: 185 MSFDPGE-WSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE 244

Query: 261 RRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPN 320
              G  E   +    ++ ++   SDEEIV  ++ +L+ GYETT+T+M L V FL  + P 
Sbjct: 245 EEEG-AERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET-PL 304

Query: 321 VLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNVVKFLHREALTDVKF 380
            LA+LKEE + IR  K +   L   DYK M FT  V++E +R  N++  + R A+TDV+ 
Sbjct: 305 ALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEI 364

Query: 381 KDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKKSSPTPSRARQSF 426
           K   IP GWKV   F A+HLDP    + + F+P RW     +  PS     F
Sbjct: 365 KGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPF 411

BLAST of HG10022188 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 4.1e-55
Identity = 123/369 (33.33%), Postives = 195/369 (52.85%), Query Frame = 0

Query: 42  NLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQHHCSRY-GKVFKSHLFGLPAIVSCDREL 101
           +LPPG  G+P +GET S+ +   SN    F+     RY   VF SHLFG  A++S D + 
Sbjct: 44  HLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQF 103

Query: 102 NVFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHS 161
           N ++LQN+ + F+  YPKA+  ++G   L+   G   RKL  + V+   + + +  F+  
Sbjct: 104 NKYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEE 163

Query: 162 LHNLSISLMASWRSQTQLLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGF 221
           + NL  S +  W  + ++    E     L++M K+LL +  ++   ++I E F  Y    
Sbjct: 164 IQNLVHSTLDRWVDKKEIALQNECHQMVLNLMAKQLLDLSPSKET-NEICELFVDYTNAV 223

Query: 222 VSLPLNLPGTPYSKAVKARGRLSYIVKEMMREKRRRGLLEGNGEDFLQVIMSNWKLSDEE 281
           +++P+ +PG+ Y+K +KAR  L   +  M++E+R    +    +D L  ++    +SDE 
Sbjct: 224 IAIPIKIPGSTYAKGLKARELLIRKISNMIKERRDHPHIV--HKDLLTKLLEEDSISDEI 283

Query: 282 IVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDY 341
           I   +L +L  G+ET++  M   + FL  + P  L ++KEE  AI   K   + L  +DY
Sbjct: 284 ICDFILFLLFAGHETSSRAMTFAIKFLT-TCPKALTQMKEEHDAILKAKGGHKKLEWDDY 343

Query: 342 KQMEFTCNVISEAMRCGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPN 401
           K M+FT  VI+E +R GN    + RE   D K KD +IP GW V    +A HLD   H  
Sbjct: 344 KSMKFTQCVINETLRLGNFGPGVFRETKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNE 403

Query: 402 PQHFDPSRW 410
              F+P RW
Sbjct: 404 ALTFNPWRW 408

BLAST of HG10022188 vs. ExPASy TrEMBL
Match: A0A0A0KNY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 2.8e-187
Identity = 334/416 (80.29%), Postives = 369/416 (88.70%), Query Frame = 0

Query: 1   MEVIGASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFL 60
           MEVIG + SPMIIIVLALS AI II H L+KLF++TS  NPNLPPG MG PFVGETLSFL
Sbjct: 1   MEVIGTNLSPMIIIVLALSFAISIIFHLLLKLFLVTSKKNPNLPPGSMGLPFVGETLSFL 60

Query: 61  KPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAM 120
            PHHSNSIGTFLQHH SRYGK+FKS LFG PAIVSCDRELN F+LQNDDKLFKVSYPKAM
Sbjct: 61  NPHHSNSIGTFLQHHFSRYGKIFKSRLFGRPAIVSCDRELNYFVLQNDDKLFKVSYPKAM 120

Query: 121 HGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLF 180
           H ILG+NSL+I+AG THRKLR+V+VSF + CK+RP+FLHSLHNLS+SL  SWRSQT + F
Sbjct: 121 HNILGTNSLLISAGDTHRKLRSVIVSFITRCKTRPNFLHSLHNLSVSLTDSWRSQTHVSF 180

Query: 181 CKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARG 240
            KE+KMFALS+MVKE+ GI+  E IG+KI EEFET+M GFVSLPLN PGTPY KAVKARG
Sbjct: 181 IKEMKMFALSLMVKEVFGIEAKELIGTKIFEEFETFMIGFVSLPLNFPGTPYFKAVKARG 240

Query: 241 RLSYIVKEMMREKRRRGLLEGNG--EDFLQVIMS-NWKLSDEEIVSVVLDILLGGYETTA 300
           RLS IVKEM+RE+R+RGL+ GN   +DFLQV+MS NWKLSDEEIVSVVLDI+LG YETTA
Sbjct: 241 RLSTIVKEMIRERRKRGLVGGNNNDDDFLQVLMSNNWKLSDEEIVSVVLDIMLGSYETTA 300

Query: 301 TLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCG 360
           TL+GLIVYFLAHSPPN+LAKLKEE QAIRNGKR GECLNLEDYK+MEFT NV  EAMRCG
Sbjct: 301 TLLGLIVYFLAHSPPNILAKLKEEHQAIRNGKRKGECLNLEDYKRMEFTFNVAYEAMRCG 360

Query: 361 NVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           NVVKFLHREA+TDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQ F+PSRW+  K
Sbjct: 361 NVVKFLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPSRWSDDK 416

BLAST of HG10022188 vs. ExPASy TrEMBL
Match: A0A6J1BWX9 (cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 1.5e-185
Identity = 336/417 (80.58%), Postives = 368/417 (88.25%), Query Frame = 0

Query: 1   MEVI--GASFSPMIIIVLALSLAI-LIICHSLIKLFVITSPPN-PNLPPGCMGFPFVGET 60
           ME++   AS SP  I+V+ALSLA+  II HSL+KL VI +  N PNLPPG MGFPF+G+T
Sbjct: 1   MELVAAAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQT 60

Query: 61  LSFLKPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSY 120
           L  LKPH SNSIGTFL HHCSRYGKVFKSHLFG PAIVSCD+ELN+FILQNDDKLFKVSY
Sbjct: 61  LGLLKPHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSY 120

Query: 121 PKAMHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQT 180
           PKAMHGILG+NSLIIAAG THRKLRNVVVSF  WCKSRPSFLH +HNL+ISLM SWRSQ 
Sbjct: 121 PKAMHGILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQN 180

Query: 181 QLLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAV 240
           Q+ FCKE+KMFALS+MVKELLGI+  E+IGSKILEEFE YMKGFVSLP+NLPGT YS AV
Sbjct: 181 QVFFCKEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAV 240

Query: 241 KARGRLSYIVKEMMREKRRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETT 300
           KAR RLS +VKEMMRE++RRG++ G  EDFLQVIMSNWKL+DEEIVSVVLDILLGGYETT
Sbjct: 241 KARERLSCMVKEMMRERKRRGMVVGADEDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 300

Query: 301 ATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRC 360
           ATLMGLIVYFLAHSPPNVLAKLKEE +AIR  KRNGE LNLEDYKQMEFT NV+ EAMRC
Sbjct: 301 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKRNGESLNLEDYKQMEFTNNVMFEAMRC 360

Query: 361 GNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           GNVVKFLHREA+ DVKFK+IVIPSGWKVHPVFSA+HLDP LHPNPQ F+P RWT  K
Sbjct: 361 GNVVKFLHREAVKDVKFKEIVIPSGWKVHPVFSAVHLDPALHPNPQQFNPWRWTDDK 417

BLAST of HG10022188 vs. ExPASy TrEMBL
Match: A0A6J1JS89 (cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 5.8e-185
Identity = 330/414 (79.71%), Postives = 366/414 (88.41%), Query Frame = 0

Query: 6   ASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHS 65
           ASFSP + I L+L++A +I+C  L+KLF   + P+P+LPPGCMGFPFVGETLSFLKPHHS
Sbjct: 5   ASFSP-LSIALSLAMAAVILCQLLLKLFA-AATPSPSLPPGCMGFPFVGETLSFLKPHHS 64

Query: 66  NSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILG 125
           NSIGTFLQ+HCSR+G VFKSHLFG PAIVSCDRELN+FILQNDDKLFKVSYPKAMHGILG
Sbjct: 65  NSIGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 124

Query: 126 SNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELK 185
           SNSLIIAAG THRKLRNVVVSF SWCKS P+FLHS+  LS+SLM SWR QT++ FCKE+K
Sbjct: 125 SNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKEVK 184

Query: 186 MFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYI 245
           MFALSVMVKELLGI+ NES+G +IL+EFETYMKGFVSLPLNLPGTPY KAVKAR +LS I
Sbjct: 185 MFALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGI 244

Query: 246 VKEMMREKRRRGLLEG------NGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 305
           VKEMM+E++++GL+ G      + E+FL VIMSNWKL +EEIVSVVLDILLGGYETTATL
Sbjct: 245 VKEMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATL 304

Query: 306 MGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNV 365
           MGLIVYFLAHSPPNVLAKLKEE +AIRNGK  GE LNLEDYKQMEFTCNVI EAMRCGNV
Sbjct: 305 MGLIVYFLAHSPPNVLAKLKEEHEAIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCGNV 364

Query: 366 VKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           VKFLHREA+ DVKFKDI IPSGWKVHPVFSAIHLDPTLHP PQ F+P RW   K
Sbjct: 365 VKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDK 416

BLAST of HG10022188 vs. ExPASy TrEMBL
Match: A0A1S3C8C0 (cytochrome P450 724B1 OS=Cucumis melo OX=3656 GN=LOC103498124 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 2.4e-183
Identity = 335/417 (80.34%), Postives = 360/417 (86.33%), Query Frame = 0

Query: 1   MEVIGASFSPMIII-VLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSF 60
           MEVIG + SPMIII VLALS AI II H L+K   + S  NPNLPPG MG PFVGETLSF
Sbjct: 1   MEVIGTNLSPMIIILVLALSFAISIIFHLLLKFLFVISKQNPNLPPGSMGLPFVGETLSF 60

Query: 61  LKPHHSNSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKA 120
           L PHHSNSIG+FLQHH SRYGK+FKSHLFG PAIVSCDRELN F+LQNDDK FKVSYPKA
Sbjct: 61  LHPHHSNSIGSFLQHHFSRYGKIFKSHLFGRPAIVSCDRELNNFVLQNDDKFFKVSYPKA 120

Query: 121 MHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLL 180
           MH ILG NSLIIAAG THRKLR+V+VSF S CK+RP FLHSLHNLS+SL+ SWRS+TQ+ 
Sbjct: 121 MHDILGKNSLIIAAGDTHRKLRSVIVSFISGCKTRPIFLHSLHNLSVSLIDSWRSRTQVS 180

Query: 181 FCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKAR 240
           F KE+KMFALS+M+KE+ GI   ESIGSKI EEFETYM GFVSLPLNLPGTPY KAVKAR
Sbjct: 181 FVKEMKMFALSLMLKEVFGIDAKESIGSKIFEEFETYMIGFVSLPLNLPGTPYFKAVKAR 240

Query: 241 GRLSYIVKEMMREKRRRGLLEG--NGEDFLQVIMS-NWKLSDEEIVSVVLDILLGGYETT 300
           GRLS IVKEMMRE+R+R LL G  N EDFLQV+MS NWKLSDEEIVSV LDILLG YETT
Sbjct: 241 GRLSTIVKEMMRERRKRELLGGNNNNEDFLQVLMSNNWKLSDEEIVSVALDILLGSYETT 300

Query: 301 ATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRC 360
           ATL+GLIVYFL HSPP +LAKLKEE QAIRNGK NGECL+LEDYKQMEFT NV  EAMRC
Sbjct: 301 ATLLGLIVYFLVHSPPKILAKLKEEHQAIRNGKGNGECLDLEDYKQMEFTYNVTYEAMRC 360

Query: 361 GNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           GNVVKFLHREA+TDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQ F+P RW   K
Sbjct: 361 GNVVKFLHREAITDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQQFNPWRWNDDK 417

BLAST of HG10022188 vs. ExPASy TrEMBL
Match: A0A6J1E2M5 (cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 5.4e-183
Identity = 330/414 (79.71%), Postives = 364/414 (87.92%), Query Frame = 0

Query: 6   ASFSPMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHS 65
           ASFSP + I L+L++A +I+C  L+KLF   + P+P+LPPGCMGFPFVGETLSFLKPHHS
Sbjct: 5   ASFSP-LTIALSLAMAAVILCQLLLKLFA-AATPSPSLPPGCMGFPFVGETLSFLKPHHS 64

Query: 66  NSIGTFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILG 125
           NSIGTFLQ HCSR+GKVFKSHLFG  AIVSCDRELN+FILQNDDKLFKVSYPKAMHGILG
Sbjct: 65  NSIGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 124

Query: 126 SNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELK 185
           SNSLIIAAG THRKLRNVVVSF SWCKS P+FLH +  LS+SLM SWRSQT++LFCKE+K
Sbjct: 125 SNSLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVK 184

Query: 186 MFALSVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYI 245
           MFALSVMVKELLGI+ NESIGS+IL+EFETYMKGFVSLPLNL GTPY KAVKAR +LS I
Sbjct: 185 MFALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLSGI 244

Query: 246 VKEMMREKRRRGLLEG------NGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATL 305
           VKEMMRE++++GL+ G      + E+FL VIMSNWKL +EE VSVVLDILLGGYETTATL
Sbjct: 245 VKEMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYETTATL 304

Query: 306 MGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNV 365
           MGLIVYFLAHSPP+VLAKLKEE + IRNGK  GE LNLEDYKQMEFTCNVI EAMRCGNV
Sbjct: 305 MGLIVYFLAHSPPHVLAKLKEEHEGIRNGKGKGEYLNLEDYKQMEFTCNVIYEAMRCGNV 364

Query: 366 VKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKK 414
           VKFLHREA+ DVKFKDI IPSGWKVHPVFSAIHLDPTLHP PQ F+P RW   K
Sbjct: 365 VKFLHREAIKDVKFKDIFIPSGWKVHPVFSAIHLDPTLHPYPQQFNPWRWRDDK 416

BLAST of HG10022188 vs. TAIR 10
Match: AT5G14400.1 (cytochrome P450, family 724, subfamily A, polypeptide 1 )

HSP 1 Score: 310.1 bits (793), Expect = 5.7e-84
Identity = 168/314 (53.50%), Postives = 216/314 (68.79%), Query Frame = 0

Query: 102 VFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSL 161
           +FILQN+ KLF   YPKAMH ILG  SL++A G  HRKL+NV++SF +  KS+P FLH  
Sbjct: 1   MFILQNEGKLFTSDYPKAMHDILGKYSLLLATGEIHRKLKNVIISFINLTKSKPDFLHCA 60

Query: 162 HNLSISLMASWRSQTQLLFCKELKMFALSVMVKELLGIKENESIGSKILEEFETYMKGFV 221
            NLSIS++ SW++  ++ F KE+K+F LSVMV +LL IK  +     +L++F +YMKGF+
Sbjct: 61  ENLSISILKSWKNCREVEFHKEVKIFTLSVMVNQLLSIKPEDPARLYVLQDFLSYMKGFI 120

Query: 222 SLPLNLPGTPYSKAVKARGRLSYIVKEMMREKRRRGLLEGNG---EDFLQVIMSNWKLSD 281
           SLP+ LPGT Y+ A+KAR RLS  V  M++E+ R      N    EDFL  I+SN  L+ 
Sbjct: 121 SLPIPLPGTGYTNAIKARKRLSARVMGMIKEREREEEDMNNAIREEDFLDSIISNEDLNY 180

Query: 282 EEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRNGECLNLE 341
           EE VS+VLDILLGG+ET+AT + L+VYFLA S PN+L KLKEE  AIR  K +GE LN E
Sbjct: 181 EEKVSIVLDILLGGFETSATTLSLVVYFLAKS-PNLLHKLKEEHAAIRAKKGDGELLNWE 240

Query: 342 DYKQMEFTCNVISEAMRCGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLH 401
           DY++MEFT  VISEA+RC                  + VIP GWKV P+F+A+HLDP+LH
Sbjct: 241 DYQKMEFTQCVISEALRC------------------EYVIPKGWKVFPIFTAVHLDPSLH 295

Query: 402 PNPQHFDPSRWTKK 413
            NP  F+P RWT K
Sbjct: 301 ENPFEFNPMRWTDK 295

BLAST of HG10022188 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 1.4e-77
Identity = 166/455 (36.48%), Postives = 266/455 (58.46%), Query Frame = 0

Query: 10  PMIIIVLALSLAILIICHSLIKLFVITSPPNPNLPPGCMGFPFVGETLSFLKPHHSNSIG 69
           P++++   LSL +      LI L         NLPPG  G+PF+GET+ +LKP+ + ++G
Sbjct: 11  PLLLLPSLLSLLLF-----LILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLG 70

Query: 70  TFLQHHCSRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILGSNSL 129
            F+Q H S+YGK+++S+LFG P IVS D  LN FILQN+ +LF+ SYP+++ GILG  S+
Sbjct: 71  DFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSM 130

Query: 130 IIAAGHTHRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELKMFAL 189
           ++  G  HR +R++ ++F S  + R   L  +   ++ ++ SW+  +      E K F  
Sbjct: 131 LVLVGDMHRDMRSISLNFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTF 190

Query: 190 SVMVKELLGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLSYIVKEM 249
           ++M K ++ +   E    ++ +E+ T+MKG VS PLNLPGT Y KA+++R  +   ++  
Sbjct: 191 NLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERK 250

Query: 250 MREKRRRGLLEGNGE-------------------------DFLQVIMSNWKLSDEEIVSV 309
           M E+R+  + E + E                         D L  ++ +  LS E+I+ +
Sbjct: 251 M-EERKLDIKEEDQEEEEVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDL 310

Query: 310 VLDILLGGYETTATLMGLIVYFLAHSPPNVLAKLKEEPQAIRNGKRN-GEC-LNLEDYKQ 369
           +L +L  G+ET++  + L ++FL  + P  + +L+EE   I   K+  GE  LN +DYK+
Sbjct: 311 ILSLLFAGHETSSVAIALAIFFL-QACPKAVEELREEHLEIARAKKELGESELNWDDYKK 370

Query: 370 MEFTCNVISEAMRCGNVVKFLHREALTDVKFKDIVIPSGWKVHPVFSAIHLDPTLHPNPQ 429
           M+FT  VI+E +R GNVV+FLHR+AL DV++K   IPSGWKV PV SA+HLD + +  P 
Sbjct: 371 MDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPN 430

Query: 430 HFDPSRWTKKKSSPTPSRARQSFTVSARRFFPSGG 438
            F+P RW +++++   S    SF+     + P GG
Sbjct: 431 LFNPWRW-QQQNNGASSSGSGSFSTWGNNYMPFGG 457

BLAST of HG10022188 vs. TAIR 10
Match: AT3G56510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 283.5 bits (724), Expect = 5.7e-76
Identity = 149/253 (58.89%), Postives = 192/253 (75.89%), Query Frame = 0

Query: 594 DAENERTGSIVRENSGEKIPESNNEKADQRNIKRKKRLLKEAGNSDMRGICYLSRVPPHM 653
           +  +E T  I  E   ++  +S  +KAD++  K K++LLKEA  +D RG+CYLSR+PPHM
Sbjct: 4   EESHELTDGISEEKESKETMKS--QKADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHM 63

Query: 654 DPLRLRQILSQYGEIQRIYLAPEDAANQVQRKRAGGFRGQFFSEGWVEFTDKRVAKKVAN 713
           D +RLR IL+QYGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA+
Sbjct: 64  DHVRLRHILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVAD 123

Query: 714 MLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERD 773
           MLNGEQIGG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+D
Sbjct: 124 MLNGEQIGGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKD 183

Query: 774 FYLAKVDKSRALNSIEERLKKKQKLQEDSEINSALADSQKLPKLIRSFPQTQPVAHSAVQ 833
           FYL+K++KSRA+  I+ R++KK+K+QE+S  N+  A     P+ I  F Q + + +   Q
Sbjct: 184 FYLSKIEKSRAMTEIDARMEKKRKIQEESGSNAEAAPVFP-PRAIFQFRQKKSIENETSQ 243

Query: 834 NKQRLSTNVLAGV 847
           +K  LST+ LA V
Sbjct: 244 SKPGLSTDFLASV 253

BLAST of HG10022188 vs. TAIR 10
Match: AT3G56510.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 283.5 bits (724), Expect = 5.7e-76
Identity = 149/253 (58.89%), Postives = 192/253 (75.89%), Query Frame = 0

Query: 594 DAENERTGSIVRENSGEKIPESNNEKADQRNIKRKKRLLKEAGNSDMRGICYLSRVPPHM 653
           +  +E T  I  E   ++  +S  +KAD++  K K++LLKEA  +D RG+CYLSR+PPHM
Sbjct: 4   EESHELTDGISEEKESKETMKS--QKADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHM 63

Query: 654 DPLRLRQILSQYGEIQRIYLAPEDAANQVQRKRAGGFRGQFFSEGWVEFTDKRVAKKVAN 713
           D +RLR IL+QYGE+ RIYLAPED+  QV RKRAGGFRGQ FSEGWVEF  K VAK+VA+
Sbjct: 64  DHVRLRHILAQYGELGRIYLAPEDSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVAD 123

Query: 714 MLNGEQIGGRKRSSFYYDLWNIKYLSKFKWDDLTEETAYKHAIREQKLALEISAAKRERD 773
           MLNGEQIGG+K+SS YYD+WNIKYL+KFKWDDLTEE AYK AIREQKL + +SAAKRE+D
Sbjct: 124 MLNGEQIGGKKKSSVYYDIWNIKYLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKD 183

Query: 774 FYLAKVDKSRALNSIEERLKKKQKLQEDSEINSALADSQKLPKLIRSFPQTQPVAHSAVQ 833
           FYL+K++KSRA+  I+ R++KK+K+QE+S  N+  A     P+ I  F Q + + +   Q
Sbjct: 184 FYLSKIEKSRAMTEIDARMEKKRKIQEESGSNAEAAPVFP-PRAIFQFRQKKSIENETSQ 243

Query: 834 NKQRLSTNVLAGV 847
           +K  LST+ LA V
Sbjct: 244 SKPGLSTDFLASV 253

BLAST of HG10022188 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-60
Identity = 142/412 (34.47%), Postives = 222/412 (53.88%), Query Frame = 0

Query: 21  AILIICHSLIKLFVI----TSPPNPNLPPGCMGFPFVGETLSFLKPHHSNSIGTFLQHHC 80
           A L++  S+   F++    T      LPPG +G P +GET   +  + + +   F+    
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 81  SRYGKVFKSHLFGLPAIVSCDRELNVFILQNDDKLFKVSYPKAMHGILGSNSLIIAAGHT 140
           +RYG VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 141 HRKLRNVVVSFTSWCKSRPSFLHSLHNLSISLMASWRSQTQLLFCKELKMFALSVMVKEL 200
           H+++ ++ +SF +    +   +  +  L    + SW S  ++L  +E K     + VK+L
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSS--RVLLMEEAKKITFELTVKQL 184

Query: 201 LGIKENESIGSKILEEFETYMKGFVSLPLNLPGTPYSKAVKARGRLS---YIVKEMMREK 260
           +     E     + +E+   ++GF SLPL L  T Y KA++AR +++    +V    RE+
Sbjct: 185 MSFDPGE-WSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE 244

Query: 261 RRRGLLEGNGEDFLQVIMSNWKLSDEEIVSVVLDILLGGYETTATLMGLIVYFLAHSPPN 320
              G  E   +    ++ ++   SDEEIV  ++ +L+ GYETT+T+M L V FL  + P 
Sbjct: 245 EEEG-AERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET-PL 304

Query: 321 VLAKLKEEPQAIRNGKRNGECLNLEDYKQMEFTCNVISEAMRCGNVVKFLHREALTDVKF 380
            LA+LKEE + IR  K +   L   DYK M FT  V++E +R  N++  + R A+TDV+ 
Sbjct: 305 ALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEI 364

Query: 381 KDIVIPSGWKVHPVFSAIHLDPTLHPNPQHFDPSRWTKKKSSPTPSRARQSF 426
           K   IP GWKV   F A+HLDP    + + F+P RW     +  PS     F
Sbjct: 365 KGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPF 411