HG10022149 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10022149 vs. NCBI nr
Match: XP_004142359.1 (GDSL esterase/lipase At3g27950 [Cucumis sativus] >KGN52374.1 hypothetical protein Csa_009178 [Cucumis sativus])

HSP 1 Score: 720.3 bits (1858), Expect = 8.7e-204
Identity = 347/381 (91.08%), Postives = 363/381 (95.28%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LR L ALLILGLVGRTV LF  AA +CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRHLWALLILGLVGRTVTLFGDAARTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDLY+RLRS  RTSIPI+ IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLYNRLRSRIRTSIPIEHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+ F EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDAFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFV 300
           NRDSNGCVKSQN VS+EFNRQLKS+LLKL KKLP ARIIHVD+YS KYLLITKA+TQGFV
Sbjct: 241 NRDSNGCVKSQNTVSREFNRQLKSQLLKLGKKLPFARIIHVDVYSVKYLLITKAKTQGFV 300

Query: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHIL 360
           KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSRHISWDGIHYS+AAN+WIANHIL
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRHISWDGIHYSEAANLWIANHIL 360

Query: 361 NGSFSDSPLPIDKACQAPSNA 382
           NGSFSD PLP+DKACQAP NA
Sbjct: 361 NGSFSDPPLPVDKACQAPRNA 381

BLAST of HG10022149 vs. NCBI nr
Match: XP_008458756.1 (PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo] >XP_008458757.1 PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo] >XP_016902334.1 PREDICTED: GDSL esterase/lipase At3g27950-like isoform X1 [Cucumis melo])

HSP 1 Score: 715.7 bits (1846), Expect = 2.1e-202
Identity = 343/381 (90.03%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LRRL ALLILGLVGRTV LF AAAG+CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDS+GTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDL++RLRS+NRT+IPIK IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+TF EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFV 300
           NRDS+GCVKSQN VS+EFNRQLKS+LLKL KKLP ARI+HVD+YS KYLLITKA++QGFV
Sbjct: 241 NRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGFV 300

Query: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHIL 360
           KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSR ISWDGIHYS+AAN WIANHIL
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHIL 360

Query: 361 NGSFSDSPLPIDKACQAPSNA 382
           +GSFSD PLP+DKACQA  +A
Sbjct: 361 DGSFSDPPLPVDKACQALRDA 381

BLAST of HG10022149 vs. NCBI nr
Match: TYK01995.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 711.1 bits (1834), Expect = 5.3e-201
Identity = 343/382 (89.79%), Postives = 366/382 (95.81%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LRRL ALLILGLVGRTV LF AAAG+CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDS+GTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDL++RLRS+NRT+IPIK IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+TF EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQG-F 300
           NRDS+GCVKSQN VS+EFNRQLKS+LLKL KKLP ARI+HVD+YS KYLLITKA++QG F
Sbjct: 241 NRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGCF 300

Query: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHI 360
           VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSR ISWDGIHYS+AAN WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHI 360

Query: 361 LNGSFSDSPLPIDKACQAPSNA 382
           L+GSFSD PLP+DKACQA  +A
Sbjct: 361 LDGSFSDPPLPVDKACQALRDA 382

BLAST of HG10022149 vs. NCBI nr
Match: XP_023551263.1 (GDSL esterase/lipase At3g27950-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 342/382 (89.53%), Postives = 362/382 (94.76%), Query Frame = 0

Query: 1   MNPLELRRLLALLILG-LVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQP 60
           M  L+LRRLL +LILG L+G T A     +G CGFPAVYNFGDSNSDTGGISAA NAIQP
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTA--GGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQP 60

Query: 61  PNGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPG 120
           PNGETFF  PSGRACDGRLI+DFIAEKLKLPYLSAYLDSLGT+FRHGANFATGGSSI PG
Sbjct: 61  PNGETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPG 120

Query: 121 GYSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQND 180
           GYSPFHLGIQVSQFIQFKSRTTDL++RL SNN+TSIPIKRIARPQEFSKALYTFDIGQND
Sbjct: 121 GYSPFHLGIQVSQFIQFKSRTTDLFNRLHSNNKTSIPIKRIARPQEFSKALYTFDIGQND 180

Query: 181 LAYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240
           LAYGYQ+SSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 DNRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGF 300
           DNRDSNGCV+SQNEVS+EFNRQLKSRLLKL KKLP+ARIIH+D+YSAKYLLIT+A+TQGF
Sbjct: 241 DNRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPVARIIHIDMYSAKYLLITEAKTQGF 300

Query: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHI 360
           VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYS+AAN+WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHI 360

Query: 361 LNGSFSDSPLPIDKACQAPSNA 382
           LNGSFSD PLPIDKAC+APS A
Sbjct: 361 LNGSFSDPPLPIDKACRAPSYA 380

BLAST of HG10022149 vs. NCBI nr
Match: XP_022938610.1 (GDSL esterase/lipase At3g27950-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 702.2 bits (1811), Expect = 2.4e-198
Identity = 342/382 (89.53%), Postives = 361/382 (94.50%), Query Frame = 0

Query: 1   MNPLELRRLLALLILG-LVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQP 60
           M  L+LRRLL +LILG L+G T A     +G CGFPAVYNFGDSNSDTGGISAA NAIQP
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTA--GGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQP 60

Query: 61  PNGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPG 120
           PNGETFF  PSGRACDGRLI+DFIAEKLKLPYLSAYLDSLGT+FRHGANFATGGSSI PG
Sbjct: 61  PNGETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPG 120

Query: 121 GYSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQND 180
           GYSPFHLGIQVSQFIQFKSR TDL++RL SNN+TSIPIKRIARPQEFSKALYTFDIGQND
Sbjct: 121 GYSPFHLGIQVSQFIQFKSRITDLFNRLHSNNKTSIPIKRIARPQEFSKALYTFDIGQND 180

Query: 181 LAYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240
           LAYGYQ+SSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 DNRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGF 300
           DNRDSNGCV+SQNEVS+EFNRQLKSRLLKL KKLPLARIIH+D+YSAKYLLIT+A+TQGF
Sbjct: 241 DNRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPLARIIHIDMYSAKYLLITEAKTQGF 300

Query: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHI 360
           VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYS+AAN+WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHI 360

Query: 361 LNGSFSDSPLPIDKACQAPSNA 382
           LNGSFSD PLPIDKAC+APS A
Sbjct: 361 LNGSFSDPPLPIDKACRAPSYA 380

BLAST of HG10022149 vs. ExPASy Swiss-Prot
Match: Q9LII9 (GDSL esterase/lipase At3g27950 OS=Arabidopsis thaliana OX=3702 GN=At3g27950 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 4.2e-108
Identity = 205/370 (55.41%), Postives = 264/370 (71.35%), Query Frame = 0

Query: 10  LALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHP 69
           LA+++L L+G T  L SA + SC FPAV+NFGDSNSDTG ISAA+  + PPNG  FFG  
Sbjct: 8   LAIIVL-LLGFTEKL-SALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRS 67

Query: 70  SGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRP--GGYSPFHLG 129
           +GR  DGRLIIDFI E L LPYL+ YLDS+G ++RHGANFATGGS IRP    +SPFHLG
Sbjct: 68  AGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLACFSPFHLG 127

Query: 130 IQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYS 189
            QVSQFI FK+RT  LY      N+T+    R++    FSKALYT DIGQNDLA G+Q  
Sbjct: 128 TQVSQFIHFKTRTLSLY------NQTNGKFNRLSHTNYFSKALYTLDIGQNDLAIGFQNM 187

Query: 190 SEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPDNRDSNGC 249
           +EEQ++A+IP I+  F+ A++ LYKEGAR+F +HNTGP GCLPY +L       RD  GC
Sbjct: 188 TEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPY-LLKAFPAIPRDPYGC 247

Query: 250 VKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFC 309
           +K  N V+ EFN+QLK+++ +L+K+LP +   +VD+YSAKY LITKA+  GF+ +P  +C
Sbjct: 248 LKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFI-DPFDYC 307

Query: 310 CGSYYGYHIDCGKREVVNGT-VYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSD 369
           C    G  + CGK   +NGT +Y + C++    ISWDGIHY++ AN+ +AN IL+GS SD
Sbjct: 308 CVGAIGRGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISD 367

Query: 370 SPLPIDKACQ 377
            PLP  KAC+
Sbjct: 368 PPLPTQKACK 367

BLAST of HG10022149 vs. ExPASy Swiss-Prot
Match: Q9LY84 (GDSL esterase/lipase At5g14450 OS=Arabidopsis thaliana OX=3702 GN=At5g14450 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.1e-103
Identity = 188/357 (52.66%), Postives = 256/357 (71.71%), Query Frame = 0

Query: 31  SCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEKLKLP 90
           +C FPA+YNFGDSNSDTGGISAA   I+ P G+ FF  P+GR  DGRL IDFIAE+L LP
Sbjct: 36  TCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGLP 95

Query: 91  YLSAYLDSLGTSFRHGANFATGGSSIR-------PGGYSPFHLGIQVSQFIQFKSRTTDL 150
           YLSAYL+SLG++FRHGANFATGGS+IR         G SPF L +Q++QF QFK+R+  L
Sbjct: 96  YLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSALL 155

Query: 151 YHRLRSN-NRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYSSEEQVRASIPDILNT 210
           + +++S  +R  +P     R +EF+KALYTFDIGQNDL+ G++  S +Q++A+IPDI+N 
Sbjct: 156 FTQIKSRYDREKLP-----RQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNH 215

Query: 211 FSEAVQQLYKEGARYFWVHNTGPIGCLPYSILY--NKSPDNRDSNGCVKSQNEVSQEFNR 270
            + AV+ +Y++G R FWVHNTGP GCLP ++ Y    +P   D +GCVK+QNE++ EFNR
Sbjct: 216 LASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNR 275

Query: 271 QLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCCGSYYGY-HIDCG 330
           +LK  ++ L K+L  A I +VD+Y+AKY +++  +  GF  NP+K CCG +  Y HI CG
Sbjct: 276 KLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFA-NPLKVCCGYHEKYDHIWCG 335

Query: 331 KREVVNGT-VYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSPLPIDKAC 376
            +  VN T +YG  C +P   +SWDG+HY++AAN  +A+  LNG  +D P+PI +AC
Sbjct: 336 NKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRAC 386

BLAST of HG10022149 vs. ExPASy Swiss-Prot
Match: B4FZ87 (GDSL esterase/lipase ACHE OS=Zea mays OX=4577 GN=ACHE PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.0e-90
Identity = 180/387 (46.51%), Postives = 250/387 (64.60%), Query Frame = 0

Query: 9   LLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGH 68
           +L LL L L        + A G C FPAV+NFGDSNSDTGG+S+   A  PPNG TFFG 
Sbjct: 15  VLLLLSLALALALRPSDAGAGGDCHFPAVFNFGDSNSDTGGLSSLFGAAPPPNGRTFFGM 74

Query: 69  PSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRP-------GGY 128
           P+GR CDGRL+IDFIAE L L +LSAYL+S+G++F  GANFAT GSSIR         G+
Sbjct: 75  PAGRYCDGRLVIDFIAESLGLTHLSAYLNSIGSNFTQGANFATAGSSIRRQNTSLFLSGF 134

Query: 129 SPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLA 188
           SP  L +Q  +F QF +R+  +Y     NN+  I  + + R + FS+ALYTFDIGQND+ 
Sbjct: 135 SPISLDVQFWEFEQFINRSQLVY-----NNKGGIYREILPRAEYFSQALYTFDIGQNDIT 194

Query: 189 YGY-QYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 248
             Y   ++ E+V A IPD++   +  +Q +Y  G RYFW+HNTGP+GCLPY++L+   PD
Sbjct: 195 SSYFVNNTTEEVEAIIPDLMERLTSIIQSVYSRGGRYFWIHNTGPLGCLPYALLHR--PD 254

Query: 249 ---NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQ 308
                D  GC  + N+V+Q FN +LK  +  L K  P A   +VD+Y+AKY LI++A   
Sbjct: 255 LAIPADGTGCSVTYNKVAQLFNLRLKETVASLRKTHPDAAFTYVDVYTAKYKLISQANKL 314

Query: 309 GFVKNPVKFCCGSYYG-YHID----CGKREVVNGT--VYGNPCKDPSRHISWDGIHYSQA 368
           GF  +P+  CCG   G Y++D    CG ++ VNGT  V G  C++PS+ +SWDG+H+++A
Sbjct: 315 GF-DDPLLTCCGYGGGRYNLDLSVGCGGKKQVNGTSVVVGKSCENPSKRVSWDGVHFTEA 374

Query: 369 ANVWIANHILNGSFSDSPLPIDKACQA 378
           AN ++ + I+ G+ SD P+ + +AC +
Sbjct: 375 ANKFVFDQIVAGALSDPPVALRQACHS 393

BLAST of HG10022149 vs. ExPASy Swiss-Prot
Match: Q7Y1X1 (Esterase OS=Hevea brasiliensis OX=3981 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.8e-88
Identity = 172/362 (47.51%), Postives = 232/362 (64.09%), Query Frame = 0

Query: 28  AAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEKL 87
           A+ +C FPA++NFGDSNSDTGG +AA   + PP GETFF   +GR  DGRLIIDFIAE  
Sbjct: 26  ASETCDFPAIFNFGDSNSDTGGKAAAFYPLNPPYGETFFHRSTGRYSDGRLIIDFIAESF 85

Query: 88  KLPYLSAYLDSLGTSFRHGANFATGGSSIR--------PGGYSPFHLGIQVSQFIQFKSR 147
            LPYLS YL SLG++F+HGA+FAT GS+I+         GG+SPF+L +Q SQF QF  R
Sbjct: 86  NLPYLSPYLSSLGSNFKHGADFATAGSTIKLPTTIIPAHGGFSPFYLDVQYSQFRQFIPR 145

Query: 148 TTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYSSEEQVRASIPDI 207
           +  +           I  + +     F KALYTFDIGQNDL  G+   + E+V A++PD+
Sbjct: 146 SQFI------RETGGIFAELVPEEYYFEKALYTFDIGQNDLTEGFLNLTVEEVNATVPDL 205

Query: 208 LNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPDNRDSNGCVKSQNEVSQEFN 267
           +N+FS  V+++Y  GAR FW+HNTGPIGCL + + Y    + +DS GC K+ NEV+Q FN
Sbjct: 206 VNSFSANVKKIYDLGARTFWIHNTGPIGCLSFILTYFPWAE-KDSAGCAKAYNEVAQHFN 265

Query: 268 RQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCC--GSYYGYHI- 327
            +LK  + +L K LPLA  +HVDIYS KY L ++ E  GF + P+  CC  G  Y + + 
Sbjct: 266 HKLKEIVAQLRKDLPLATFVHVDIYSVKYSLFSEPEKHGF-EFPLITCCGYGGKYNFSVT 325

Query: 328 -DCGKREVV-NGT-VYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSPLPIDK 376
             CG      +GT +    C  PS  ++WDG HY++AAN +  + I  G+FSD P+P++ 
Sbjct: 326 APCGDTVTADDGTKIVVGSCACPSVRVNWDGAHYTEAANEYFFDQISTGAFSDPPVPLNM 379

BLAST of HG10022149 vs. ExPASy Swiss-Prot
Match: Q9LIN2 (GDSL esterase/lipase At3g26430 OS=Arabidopsis thaliana OX=3702 GN=At3g26430 PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 8.1e-88
Identity = 167/364 (45.88%), Postives = 237/364 (65.11%), Query Frame = 0

Query: 27  AAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEK 86
           A + SC FPA++NFGDSNSDTGG+SA+      PNG+TFF  PSGR  DGRLIIDFIAE+
Sbjct: 22  ACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSDGRLIIDFIAEE 81

Query: 87  LKLPYLSAYLDSLGTSFRHGANFATGGSSIRP-------GGYSPFHLGIQVSQFIQFKSR 146
           L LPYL+A+LDS+G++F HGANFAT GS++RP        G SP  L +Q+ QF  F +R
Sbjct: 82  LGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDVQLVQFSDFITR 141

Query: 147 TTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYS-SEEQVRASIPD 206
           +  +       NR  +  K + + + FS+ALYTFDIGQNDL  G + + + +Q++A IPD
Sbjct: 142 SQLI------RNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQIKAYIPD 201

Query: 207 ILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPDNR-DSNGCVKSQNEVSQE 266
           + +  S  ++++Y +G R FW+HNT P+GCLPY +     P ++ D++GC   +NE+++ 
Sbjct: 202 VHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNHGCAIPRNEIARY 261

Query: 267 FNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCCGSYYGYH-- 326
           +N +LK R+++L K+L  A   +VDIYS K  LIT+A+  GF + P+  CCG    Y+  
Sbjct: 262 YNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF-RYPLVACCGHGGKYNFN 321

Query: 327 --IDCGKREVVNG--TVYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSPLPI 376
             I CG + ++ G   V    C D S  +SWDGIH+++  N WI   I +G+FSD PLP+
Sbjct: 322 KLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQINDGAFSDPPLPV 378

BLAST of HG10022149 vs. ExPASy TrEMBL
Match: A0A0A0KTW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G630770 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 4.2e-204
Identity = 347/381 (91.08%), Postives = 363/381 (95.28%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LR L ALLILGLVGRTV LF  AA +CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRHLWALLILGLVGRTVTLFGDAARTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDLY+RLRS  RTSIPI+ IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLYNRLRSRIRTSIPIEHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+ F EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDAFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFV 300
           NRDSNGCVKSQN VS+EFNRQLKS+LLKL KKLP ARIIHVD+YS KYLLITKA+TQGFV
Sbjct: 241 NRDSNGCVKSQNTVSREFNRQLKSQLLKLGKKLPFARIIHVDVYSVKYLLITKAKTQGFV 300

Query: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHIL 360
           KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSRHISWDGIHYS+AAN+WIANHIL
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRHISWDGIHYSEAANLWIANHIL 360

Query: 361 NGSFSDSPLPIDKACQAPSNA 382
           NGSFSD PLP+DKACQAP NA
Sbjct: 361 NGSFSDPPLPVDKACQAPRNA 381

BLAST of HG10022149 vs. ExPASy TrEMBL
Match: A0A1S4E275 (GDSL esterase/lipase At3g27950-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498068 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.0e-202
Identity = 343/381 (90.03%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LRRL ALLILGLVGRTV LF AAAG+CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDS+GTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDL++RLRS+NRT+IPIK IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+TF EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFV 300
           NRDS+GCVKSQN VS+EFNRQLKS+LLKL KKLP ARI+HVD+YS KYLLITKA++QGFV
Sbjct: 241 NRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGFV 300

Query: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHIL 360
           KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSR ISWDGIHYS+AAN WIANHIL
Sbjct: 301 KNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHIL 360

Query: 361 NGSFSDSPLPIDKACQAPSNA 382
           +GSFSD PLP+DKACQA  +A
Sbjct: 361 DGSFSDPPLPVDKACQALRDA 381

BLAST of HG10022149 vs. ExPASy TrEMBL
Match: A0A5D3BS38 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G001090 PE=3 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 2.5e-201
Identity = 343/382 (89.79%), Postives = 366/382 (95.81%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LRRL ALLILGLVGRTV LF AAAG+CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDS+GTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDL 180
           YSPFHLGIQVSQFIQFKSRTTDL++RLRS+NRT+IPIK IARPQEFSKALYTFDIGQNDL
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHNRTAIPIKHIARPQEFSKALYTFDIGQNDL 180

Query: 181 AYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPD 240
           AYGYQ+SSEEQVRASIPDIL+TF EAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP+
Sbjct: 181 AYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPE 240

Query: 241 NRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQG-F 300
           NRDS+GCVKSQN VS+EFNRQLKS+LLKL KKLP ARI+HVD+YS KYLLITKA++QG F
Sbjct: 241 NRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARIVHVDMYSVKYLLITKAKSQGCF 300

Query: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHI 360
           VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPSR ISWDGIHYS+AAN WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPSRQISWDGIHYSEAANSWIANHI 360

Query: 361 LNGSFSDSPLPIDKACQAPSNA 382
           L+GSFSD PLP+DKACQA  +A
Sbjct: 361 LDGSFSDPPLPVDKACQALRDA 382

BLAST of HG10022149 vs. ExPASy TrEMBL
Match: A0A6J1FK92 (GDSL esterase/lipase At3g27950-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444793 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.2e-198
Identity = 342/382 (89.53%), Postives = 361/382 (94.50%), Query Frame = 0

Query: 1   MNPLELRRLLALLILG-LVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQP 60
           M  L+LRRLL +LILG L+G T A     +G CGFPAVYNFGDSNSDTGGISAA NAIQP
Sbjct: 1   MGLLKLRRLLVVLILGLLIGVTTA--GGGSGKCGFPAVYNFGDSNSDTGGISAAFNAIQP 60

Query: 61  PNGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPG 120
           PNGETFF  PSGRACDGRLI+DFIAEKLKLPYLSAYLDSLGT+FRHGANFATGGSSI PG
Sbjct: 61  PNGETFFRRPSGRACDGRLIVDFIAEKLKLPYLSAYLDSLGTNFRHGANFATGGSSICPG 120

Query: 121 GYSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQND 180
           GYSPFHLGIQVSQFIQFKSR TDL++RL SNN+TSIPIKRIARPQEFSKALYTFDIGQND
Sbjct: 121 GYSPFHLGIQVSQFIQFKSRITDLFNRLHSNNKTSIPIKRIARPQEFSKALYTFDIGQND 180

Query: 181 LAYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240
           LAYGYQ+SSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP
Sbjct: 181 LAYGYQHSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSP 240

Query: 241 DNRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGF 300
           DNRDSNGCV+SQNEVS+EFNRQLKSRLLKL KKLPLARIIH+D+YSAKYLLIT+A+TQGF
Sbjct: 241 DNRDSNGCVESQNEVSREFNRQLKSRLLKLGKKLPLARIIHIDMYSAKYLLITEAKTQGF 300

Query: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSQAANVWIANHI 360
           VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYS+AAN+WIANHI
Sbjct: 301 VKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPSRHISWDGIHYSEAANLWIANHI 360

Query: 361 LNGSFSDSPLPIDKACQAPSNA 382
           LNGSFSD PLPIDKAC+APS A
Sbjct: 361 LNGSFSDPPLPIDKACRAPSYA 380

BLAST of HG10022149 vs. ExPASy TrEMBL
Match: A0A5A7T2C7 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G001330 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.5e-197
Identity = 342/404 (84.65%), Postives = 366/404 (90.59%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MN L+LRRL ALLILGLVGRTV LF AAAG+CGFPA+YNFGDSNSDTGGISAALNAIQPP
Sbjct: 1   MNSLKLRRLWALLILGLVGRTVTLFGAAAGTCGFPAIYNFGDSNSDTGGISAALNAIQPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRPGG 120
           NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDS+GTSFRHGANFATGGSSIRPGG
Sbjct: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSIGTSFRHGANFATGGSSIRPGG 120

Query: 121 YSPFHLGIQVSQFIQFKSRTTDLYHRLRSNN----------------------RTSIPIK 180
           YSPFHLGIQVSQFIQFKSRTTDL++RLRS++                      RT+IPIK
Sbjct: 121 YSPFHLGIQVSQFIQFKSRTTDLFNRLRSHSQFLILYSLHYTSQDRTELYLLYRTAIPIK 180

Query: 181 RIARPQEFSKALYTFDIGQNDLAYGYQYSSEEQVRASIPDILNTFSEAVQQLYKEGARYF 240
            IARPQEFSKALYTFDIGQNDLAYGYQ+SSEEQVRASIPDIL+TF EAVQQLYKEGARYF
Sbjct: 181 HIARPQEFSKALYTFDIGQNDLAYGYQHSSEEQVRASIPDILDTFCEAVQQLYKEGARYF 240

Query: 241 WVHNTGPIGCLPYSILYNKSPDNRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARI 300
           WVHNTGPIGCLPYSILYNKSP+NRDS+GCVKSQN VS+EFNRQLKS+LLKL KKLP ARI
Sbjct: 241 WVHNTGPIGCLPYSILYNKSPENRDSSGCVKSQNTVSREFNRQLKSQLLKLRKKLPFARI 300

Query: 301 IHVDIYSAKYLLITKAETQG-FVKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCKDPS 360
           +HVD+YS KYLLITKA++QG FVKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPC+DPS
Sbjct: 301 VHVDMYSVKYLLITKAKSQGCFVKNPVKFCCGSYYGYHIDCGKREVVNGTVYGNPCEDPS 360

Query: 361 RHISWDGIHYSQAANVWIANHILNGSFSDSPLPIDKACQAPSNA 382
           R ISWDGIHYS+AAN WIANHIL+GSFSD PLP+DKACQA  +A
Sbjct: 361 RQISWDGIHYSEAANSWIANHILDGSFSDPPLPVDKACQALRDA 404

BLAST of HG10022149 vs. TAIR 10
Match: AT3G27950.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.7e-107
Identity = 204/370 (55.14%), Postives = 262/370 (70.81%), Query Frame = 0

Query: 10  LALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHP 69
           LA+++L L+G T  L SA + SC FPAV+NFGDSNSDTG ISAA+  + PPNG  FFG  
Sbjct: 8   LAIIVL-LLGFTEKL-SALSSSCNFPAVFNFGDSNSDTGAISAAIGEVPPPNGVAFFGRS 67

Query: 70  SGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGGSSIRP--GGYSPFHLG 129
           +GR  DGRLIIDFI E L LPYL+ YLDS+G ++RHGANFATGGS IRP    +SPFHLG
Sbjct: 68  AGRHSDGRLIIDFITENLTLPYLTPYLDSVGANYRHGANFATGGSCIRPTLACFSPFHLG 127

Query: 130 IQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYS 189
            QVSQFI FK+RT  LY      N+T+          +FSKALYT DIGQNDLA G+Q  
Sbjct: 128 TQVSQFIHFKTRTLSLY------NQTN----------DFSKALYTLDIGQNDLAIGFQNM 187

Query: 190 SEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPDNRDSNGC 249
           +EEQ++A+IP I+  F+ A++ LYKEGAR+F +HNTGP GCLPY +L       RD  GC
Sbjct: 188 TEEQLKATIPLIIENFTIALKLLYKEGARFFSIHNTGPTGCLPY-LLKAFPAIPRDPYGC 247

Query: 250 VKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFC 309
           +K  N V+ EFN+QLK+++ +L+K+LP +   +VD+YSAKY LITKA+  GF+ +P  +C
Sbjct: 248 LKPLNNVAIEFNKQLKNKITQLKKELPSSFFTYVDVYSAKYNLITKAKALGFI-DPFDYC 307

Query: 310 CGSYYGYHIDCGKREVVNGT-VYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSD 369
           C    G  + CGK   +NGT +Y + C++    ISWDGIHY++ AN+ +AN IL+GS SD
Sbjct: 308 CVGAIGRGMGCGKTIFLNGTELYSSSCQNRKNFISWDGIHYTETANMLVANRILDGSISD 357

Query: 370 SPLPIDKACQ 377
            PLP  KAC+
Sbjct: 368 PPLPTQKACK 357

BLAST of HG10022149 vs. TAIR 10
Match: AT5G14450.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 7.5e-105
Identity = 188/357 (52.66%), Postives = 256/357 (71.71%), Query Frame = 0

Query: 31  SCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEKLKLP 90
           +C FPA+YNFGDSNSDTGGISAA   I+ P G+ FF  P+GR  DGRL IDFIAE+L LP
Sbjct: 36  TCTFPAIYNFGDSNSDTGGISAAFEPIRDPYGQGFFHRPTGRDSDGRLTIDFIAERLGLP 95

Query: 91  YLSAYLDSLGTSFRHGANFATGGSSIR-------PGGYSPFHLGIQVSQFIQFKSRTTDL 150
           YLSAYL+SLG++FRHGANFATGGS+IR         G SPF L +Q++QF QFK+R+  L
Sbjct: 96  YLSAYLNSLGSNFRHGANFATGGSTIRRQNETIFQYGISPFSLDMQIAQFDQFKARSALL 155

Query: 151 YHRLRSN-NRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYSSEEQVRASIPDILNT 210
           + +++S  +R  +P     R +EF+KALYTFDIGQNDL+ G++  S +Q++A+IPDI+N 
Sbjct: 156 FTQIKSRYDREKLP-----RQEEFAKALYTFDIGQNDLSVGFRTMSVDQLKATIPDIVNH 215

Query: 211 FSEAVQQLYKEGARYFWVHNTGPIGCLPYSILY--NKSPDNRDSNGCVKSQNEVSQEFNR 270
            + AV+ +Y++G R FWVHNTGP GCLP ++ Y    +P   D +GCVK+QNE++ EFNR
Sbjct: 216 LASAVRNIYQQGGRTFWVHNTGPFGCLPVNMFYMGTPAPGYLDKSGCVKAQNEMAMEFNR 275

Query: 271 QLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCCGSYYGY-HIDCG 330
           +LK  ++ L K+L  A I +VD+Y+AKY +++  +  GF  NP+K CCG +  Y HI CG
Sbjct: 276 KLKETVINLRKELTQAAITYVDVYTAKYEMMSNPKKLGFA-NPLKVCCGYHEKYDHIWCG 335

Query: 331 KREVVNGT-VYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSPLPIDKAC 376
            +  VN T +YG  C +P   +SWDG+HY++AAN  +A+  LNG  +D P+PI +AC
Sbjct: 336 NKGKVNNTEIYGGSCPNPVMAVSWDGVHYTEAANKHVADRTLNGLLTDPPVPITRAC 386

BLAST of HG10022149 vs. TAIR 10
Match: AT3G26430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 5.8e-89
Identity = 167/364 (45.88%), Postives = 237/364 (65.11%), Query Frame = 0

Query: 27  AAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEK 86
           A + SC FPA++NFGDSNSDTGG+SA+      PNG+TFF  PSGR  DGRLIIDFIAE+
Sbjct: 22  ACSPSCNFPAIFNFGDSNSDTGGLSASFGQAPYPNGQTFFHSPSGRFSDGRLIIDFIAEE 81

Query: 87  LKLPYLSAYLDSLGTSFRHGANFATGGSSIRP-------GGYSPFHLGIQVSQFIQFKSR 146
           L LPYL+A+LDS+G++F HGANFAT GS++RP        G SP  L +Q+ QF  F +R
Sbjct: 82  LGLPYLNAFLDSIGSNFSHGANFATAGSTVRPPNATIAQSGVSPISLDVQLVQFSDFITR 141

Query: 147 TTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTFDIGQNDLAYGYQYS-SEEQVRASIPD 206
           +  +       NR  +  K + + + FS+ALYTFDIGQNDL  G + + + +Q++A IPD
Sbjct: 142 SQLI------RNRGGVFKKLLPKKEYFSQALYTFDIGQNDLTAGLKLNMTSDQIKAYIPD 201

Query: 207 ILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYSILYNKSPDNR-DSNGCVKSQNEVSQE 266
           + +  S  ++++Y +G R FW+HNT P+GCLPY +     P ++ D++GC   +NE+++ 
Sbjct: 202 VHDQLSNVIRKVYSKGGRRFWIHNTAPLGCLPYVLDRFPVPASQIDNHGCAIPRNEIARY 261

Query: 267 FNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCCGSYYGYH-- 326
           +N +LK R+++L K+L  A   +VDIYS K  LIT+A+  GF + P+  CCG    Y+  
Sbjct: 262 YNSELKRRVIELRKELSEAAFTYVDIYSIKLTLITQAKKLGF-RYPLVACCGHGGKYNFN 321

Query: 327 --IDCGKREVVNG--TVYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSPLPI 376
             I CG + ++ G   V    C D S  +SWDGIH+++  N WI   I +G+FSD PLP+
Sbjct: 322 KLIKCGAKVMIKGKEIVLAKSCNDVSFRVSWDGIHFTETTNSWIFQQINDGAFSDPPLPV 378

BLAST of HG10022149 vs. TAIR 10
Match: AT1G67830.1 (alpha-fucosidase 1 )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 180/392 (45.92%), Postives = 241/392 (61.48%), Query Frame = 0

Query: 1   MNPLELRRLLALLILGLVGRTVALFSAAAGSCGFPAVYNFGDSNSDTGGISAALNAIQPP 60
           MNP+ L  L AL +L  +       S  A  C FPA++NFGDSNSDTGG+SAA     PP
Sbjct: 1   MNPI-LSSLFALSLLSSLSP-----STHAHQCHFPAIFNFGDSNSDTGGLSAAFGQAGPP 60

Query: 61  NGETFFGHPSGRACDGRLIIDFIAEKLKLPYLSAYLDSLGTSFRHGANFATGG------- 120
           +G +FFG P+GR CDGRL+IDFIAE L LPYLSA+LDS+G++F HGANFAT G       
Sbjct: 61  HGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLSAFLDSVGSNFSHGANFATAGSPIRALN 120

Query: 121 SSIRPGGYSPFHLGIQVSQFIQFKSRTTDLYHRLRSNNRTSIPIKRIARPQEFSKALYTF 180
           S++R  G+SPF L +Q  QF  F +R+  +       +R  +    +     FSKALYTF
Sbjct: 121 STLRQSGFSPFSLDVQFVQFYNFHNRSQTV------RSRGGVYKTMLPESDSFSKALYTF 180

Query: 181 DIGQNDLAYGY-QYSSEEQVRASIPDILNTFSEAVQQLYKEGARYFWVHNTGPIGCLPYS 240
           DIGQNDL  GY    + EQV   +P+I++ F  A++ +Y +G RYFW+HNTGPIGCL Y 
Sbjct: 181 DIGQNDLTAGYFANKTVEQVETEVPEIISQFMNAIKNIYGQGGRYFWIHNTGPIGCLAYV 240

Query: 241 I--LYNKSPDNRDSNGCVKSQNEVSQEFNRQLKSRLLKLEKKLPLARIIHVDIYSAKYLL 300
           I    NK+ D  DS+GCV   N ++Q+FN  LK  +++L   L  A I +VD+YS K+ L
Sbjct: 241 IERFPNKASD-FDSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVYSLKHEL 300

Query: 301 ITKAETQGFVKNPVKFCC--GSYYGYH--IDCGKREVVNG-TVY-GNPCKDPSRHISWDG 360
              A+  GF K  +  CC  G  Y Y+  I CG +++V G  VY G PC +P + + WDG
Sbjct: 301 FVHAQGHGF-KGSLVSCCGHGGKYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDKAVVWDG 360

Query: 361 IHYSQAANVWIANHILNGSFSDSPLPIDKACQ 377
           +H++QAAN +I + I  G        + KAC+
Sbjct: 361 VHFTQAANKFIFDKIAPG--------LSKACK 370

BLAST of HG10022149 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 154/348 (44.25%), Postives = 216/348 (62.07%), Query Frame = 0

Query: 34  FPAVYNFGDSNSDTGG-ISAALNAIQPPNGETFFGHPSGRACDGRLIIDFIAEKLKLPYL 93
           +PA+ NFGDSNSDTG  ISA +  + PP G+T+F  PSGR CDGRLI+DF+ +++ LP+L
Sbjct: 30  YPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRYCDGRLIVDFLLDEMDLPFL 89

Query: 94  SAYLDSLG-TSFRHGANFATGGSSI---RPGGYSPFHLGIQVSQFIQFKSRTTDLYHRLR 153
           + YLDSLG  +F+ G NFA  GS+I    P   SPF   +Q+SQFI+FKSR  +L     
Sbjct: 90  NPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQISQFIRFKSRAIELL---- 149

Query: 154 SNNRTSIPIKRIARPQE-FSKALYTFDIGQNDLAYGYQYSSEEQVRASIPDILNTFSEAV 213
             ++T    ++   P + +SK LY  DIGQND+A  +   + +QV ASIP IL TF   +
Sbjct: 150 --SKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSKTLDQVLASIPSILETFEAGL 209

Query: 214 QQLYKEGARYFWVHNTGPIGCLPYSIL-YNKSPDNRDSNGCVKSQNEVSQEFNRQLKSRL 273
           ++LY+EG R  W+HNTGP+GCL  +I  +       D  GCV S N+ ++ FN QL +  
Sbjct: 210 KRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMS 269

Query: 274 LKLEKKLPLARIIHVDIYSAKYLLITKAETQGFVKNPVKFCCG-----SYYGYHIDCGKR 333
            K + + P A + +VDI+S K  LI      GF K P+  CCG       Y   I CG+ 
Sbjct: 270 NKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEK-PLMACCGVGGAPLNYDSRITCGQT 329

Query: 334 EVVNG-TVYGNPCKDPSRHISWDGIHYSQAANVWIANHILNGSFSDSP 369
           +V++G +V    C D S +I+WDGIHY++AAN ++++ IL G +SD P
Sbjct: 330 KVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPP 370