HG10022071 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10022071
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Defective in cullin neddylation protein
Location	Chr05: 20483816 .. 20486007 (+)
RNA-Seq Expression	HG10022071
Synteny	HG10022071

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGGTATCCTTTTCCCCCTTCTCTTTCTTTCCTTTTCTTTAGAACTTAAGGACACAGTGATACAACCTATAGAAAGATCTTGTCCAAATTAGGTATTTTCCTTTTTGTTAAGGTACCCTTTTTCCCCATTCTCTTTTGTGCTGCTACTTAAGTATAGTATTAATAATCAGGAAACCAGAATTTCTACCTGAAATCATTTTCATCAACTTGGGGTTATGGGGCCTAACAATTATGAAACATACGTACATGCTAAATACCCATGAATGAACATTTTAATGTAGTTGCTATCTGCAGACATTTGGATACACGTTCATATATATATTTTACAAAAATGCATGCATTTATGTTTGAATTAGATGAATTTGGAAAATTTTGGGTATTTTTGTACCTAAGCTAAATCTACCAGATTTCTTCCATCTGGGTTTGTGCATTCATGTTATTTTTTTGGTGTTTTATCCTTCAACTTGTTCCTTGAATTAGTAGTGCTATATAGTGCCACTTATCTCCAAGGATCTGATGTATTTAAGTTCCACAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTATGATCCCGAAGGTATTTGTTTCTTTTTTTCTTTTTTTTCTTTTTTGCAATGGAAGTAATTTATTAGATATTATTTGAATTAAGAATGTTAAGACATGGGAATTACGTCAAGTAATCCTTCTCCTTAACTGTTGAAGTTTTTTCGCTGATACAATTGAATTCTATCACAAGAAAAAGTATATTTTGTCAATTTATATAAGAAAAATATAATCCTACACTTTCATGTTTAACCTTCTGTTTTGGTTCTTCTATGGAGGCTTGAAATATGATGAGAATGAAAATATTTATTGATCTCATTATATTGTTATGGTGGCTTGGAATTGTTGTTTCTCAAGTGTATGCCTAATGCCTAAAAACATTTTTGAGGCTTGAGTTCCAAATAGTTATATGATGGGTGGAATAATACAATTAACAGCTGTCAGCATATTCTTCAGATAATGGAAGTCCATTTCTGAATGCATGGATATATAACACTGATTGTGTTAATATAGCCCTTAAGTACTTTTATCCTATGAAAAATACAGAAAACAAAAGTTAAAGAAGAGATTCATGGTCTTGCAGTGGATGGATACTTGATTTTTTTTTCGGTACTTTTATAATCATTGTGTTTAATACTGCATGGTTCTGTATTTTTCTCAAAATTTTCCAATTAACTAGAGGATTATCACTTCTCTTGAAGAAAGAGCCTCAAGACTAGCCCTTACTCTTTTGAAATGTTGTGTATGCTTTTAAATAAGCAGAATGCTTCTCTTTTCTTACTGTAATGTTTGGACTGACTCAGTTGCGTTCTTCTATCAGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGTTCGTTTGTGGAGGTTCTTACAGTTGCAACAAGTTTGAAGTTTAATGTAGTTAAATAGTTCTGGGTTATATATCATTTTAAGTCCTCGTTATTATCATGGTTTAGCCTTGTACTAACACTTGGTCTGCTTATAATTATTGTGCAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGTATAGAAAAAAAATGTACCATTCTACATATGGATTTCCATGGCATGCAGAATGTTATATAAGCCGTCTTTTTCTGGTTGGTATTGTGTCAGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGAAAAGCGTCATTCACGTGA

mRNA sequence

ATGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTATGATCCCGAAGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGAAAAGCGTCATTCACGTGA

Coding sequence (CDS)

ATGGCAGTTAGAGCATGGAGATTAGTTTTGGATGGGAGATTTCGGTTACTTAATCAATGGTGTGACTTTGTTGAGAACAATCAGCGACACAATATTTCTGAGGATACTTGGCAGCAAGTCTTGGCTTTCAGCCGCTGTGTACATGAAAATCTGGAAGGGTATGATCCCGAAGGTGCTTGGCCTGTTCTAATTGATGACTTCGTTGAGCACATGTACAGGCGGTCAGGGTCTAATAAAGTCTCAAAGTTATGTTGTAATTGTGGAGACTCAGAATCACAGTCAGGTGTATTTGAAGATTCTCTTTCTGGATTAAAGTTTTTCCCTGGTTTGAAGAGGAAATTACCTGAGGACATGCAAATGGACGGCATTGTGTCTCTGAGTGATCCTAGAACCGGCTCCATGGAGTTAAGTCCTGTTACGAGCATCAAGAAAAGTAGATTCATGGCTTGCAGGCCAGTGAACTTGGAGATAAACTTACCATCATATACTGTAGATGAGAACATAGAAATGGTTCGTGCGGTAGGTTCCAAATCTCCATGTGCTGTTGAAGGTTGTCTGTCAAAAGGTTTTGCTGGGCTTTTCTCGACTCGTTTGTTCTTAGGATTGGACCAAGAAAGAAAAGCGTCATTCACGTGA

Protein sequence

MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWPVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVRAVGSKSPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT

Homology

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BLAST of HG10022071 vs. NCBI nr
Match: XP_038890437.1 (uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida])

HSP 1 Score: 417.9 bits (1073), Expect = 5.1e-113
Identity = 201/214 (93.93%), Postives = 205/214 (95.79%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVL+GRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 113 MAVRAWRLVLNGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 172

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYR+SGSNKVSK CCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 173 PVLIDDFVEHMYRQSGSNKVSKFCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 232

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGI+S SDPRTGSMELSPV S+KKSRFMACRPVNLEINLPS T DEN EMVR   AVGSK
Sbjct: 233 DGIMSPSDPRTGSMELSPVLSVKKSRFMACRPVNLEINLPSCTADENTEMVRHNSAVGSK 292

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 293 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 326

[Close]

BLAST of HG10022071 vs. NCBI nr
Match: XP_038890438.1 (uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida])

HSP 1 Score: 417.9 bits (1073), Expect = 5.1e-113
Identity = 201/214 (93.93%), Postives = 205/214 (95.79%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVL+GRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 95  MAVRAWRLVLNGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 154

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYR+SGSNKVSK CCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 155 PVLIDDFVEHMYRQSGSNKVSKFCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 214

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGI+S SDPRTGSMELSPV S+KKSRFMACRPVNLEINLPS T DEN EMVR   AVGSK
Sbjct: 215 DGIMSPSDPRTGSMELSPVLSVKKSRFMACRPVNLEINLPSCTADENTEMVRHNSAVGSK 274

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 275 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 308

[Close]

BLAST of HG10022071 vs. NCBI nr
Match: KAA0039495.1 (defective in cullin neddylation protein 1 [Cucumis melo var. makuwa] >TYK15249.1 defective in cullin neddylation protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 414.8 bits (1065), Expect = 4.3e-112
Identity = 199/214 (92.99%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKLCC CGDSES+SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 61  PVLIDDFVEHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQM 120

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRTG+ME +PVTS+KKSRFMACRPVNLEINLPS T  ENIE+VR    VGSK
Sbjct: 121 DGIVSPSDPRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVGSK 180

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 214

[Close]

BLAST of HG10022071 vs. NCBI nr
Match: XP_008459316.1 (PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucumis melo])

HSP 1 Score: 414.8 bits (1065), Expect = 4.3e-112
Identity = 199/214 (92.99%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 112 MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 171

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKLCC CGDSES+SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 172 PVLIDDFVEHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQM 231

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRTG+ME +PVTS+KKSRFMACRPVNLEINLPS T  ENIE+VR    VGSK
Sbjct: 232 DGIVSPSDPRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVGSK 291

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 292 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 325

[Close]

BLAST of HG10022071 vs. NCBI nr
Match: XP_004148700.1 (uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical protein Csa_007882 [Cucumis sativus])

HSP 1 Score: 404.1 bits (1037), Expect = 7.6e-109
Identity = 197/214 (92.06%), Postives = 201/214 (93.93%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 112 MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 171

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKL CNCGDSE +SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 172 PVLIDDFVEHMYRRSGSNKVSKLSCNCGDSE-ESGVFEDSLSGLKFFPGLKRKLPEDMQM 231

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRT +ME +PVTS+KKSRFMACRPVNLEIN PS T  ENIEMVR    VGSK
Sbjct: 232 DGIVSPSDPRTSAMEFTPVTSVKKSRFMACRPVNLEINSPSCTAAENIEMVRHNSTVGSK 291

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 292 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 324

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BLAST of HG10022071 vs. ExPASy Swiss-Prot
Match: Q9MBG8 (Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=AAR3 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-47
Identity = 103/207 (49.76%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 2   AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWP 61
           A+ AW+LVL GRFRLLN+WCDF+E NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWP
Sbjct: 110 AITAWKLVLAGRFRLLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWP 169

Query: 62  VLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDS---------LSGLKFFPGLKR 121
           VLIDDFVEHMY   G NK + L C CGD+ES+S ++++           +GL+  PGLKR
Sbjct: 170 VLIDDFVEHMYSILGPNKDTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKR 229

Query: 122 KLP---EDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIE 181
           K     ++ + D    + + +  S  L+    IK S    C                   
Sbjct: 230 KTSKKNDEEEEDEDEEVLETQNSSSLLN-FKRIKTSNSPRC------------------- 289

Query: 182 MVRAVGSKSPCAVEGCLSKGFAGLFST 197
                 SKSPC++E  LS+GFA L ST
Sbjct: 290 -----SSKSPCSIERSLSQGFASLLST 291

[Close]

BLAST of HG10022071 vs. ExPASy Swiss-Prot
Match: Q9VUQ8 (DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.6e-16
Identity = 35/69 (50.72%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MA+  W +VL GRF+ L+ WC F+E   +  IS DTW  +L F+  + + +  YD EGAW
Sbjct: 173 MAIAYWCIVLSGRFKFLDIWCQFLEEKHKRAISRDTWNLLLDFATNIDDRMSNYDSEGAW 232

Query: 61  PVLIDDFVE 70
           PVLIDDFVE
Sbjct: 233 PVLIDDFVE 241

[Close]

BLAST of HG10022071 vs. ExPASy Swiss-Prot
Match: Q8BZJ7 (DCN1-like protein 2 OS=Mus musculus OX=10090 GN=Dcun1d2 PE=1 SV=3)

HSP 1 Score: 83.6 bits (205), Expect = 3.0e-15
Identity = 35/70 (50.00%), Postives = 47/70 (67.14%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAV  W+LVL GRF+ L+ W  F+  + + +I  DTW  +L F   + ++L  YD EGAW
Sbjct: 177 MAVAYWKLVLSGRFKFLDLWNTFLLEHHKRSIPRDTWNLLLDFGNMIADDLSNYDEEGAW 236

Query: 61  PVLIDDFVEH 71
           PVLIDDFVE+
Sbjct: 237 PVLIDDFVEY 246

[Close]

BLAST of HG10022071 vs. ExPASy Swiss-Prot
Match: Q6PH85 (DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.9e-15
Identity = 34/70 (48.57%), Postives = 47/70 (67.14%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAV  W+LVL GRF+ L+ W  F+  + + +I  DTW  +L F   + +++  YD EGAW
Sbjct: 177 MAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAW 236

Query: 61  PVLIDDFVEH 71
           PVLIDDFVE+
Sbjct: 237 PVLIDDFVEY 246

[Close]

BLAST of HG10022071 vs. ExPASy Swiss-Prot
Match: Q5ZKU1 (DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.1e-15
Identity = 34/69 (49.28%), Postives = 48/69 (69.57%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MA+  W LVL+GRF+ L+ W  F+  + + +I +DTW  +L FS  + +++  YD EGAW
Sbjct: 177 MAIAYWNLVLNGRFKFLDLWNKFLLEHHKRSIPKDTWNLLLDFSTMIADDMSNYDEEGAW 236

Query: 61  PVLIDDFVE 70
           PVLIDDFVE
Sbjct: 237 PVLIDDFVE 245

[Close]

BLAST of HG10022071 vs. ExPASy TrEMBL
Match: A0A5A7TDI6 (Defective in cullin neddylation protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00230 PE=4 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.1e-112
Identity = 199/214 (92.99%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKLCC CGDSES+SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 61  PVLIDDFVEHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQM 120

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRTG+ME +PVTS+KKSRFMACRPVNLEINLPS T  ENIE+VR    VGSK
Sbjct: 121 DGIVSPSDPRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVGSK 180

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 214

[Close]

BLAST of HG10022071 vs. ExPASy TrEMBL
Match: A0A1S3CB35 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 PE=4 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.1e-112
Identity = 199/214 (92.99%), Postives = 204/214 (95.33%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 112 MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 171

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKLCC CGDSES+SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 172 PVLIDDFVEHMYRRSGSNKVSKLCCTCGDSESESGVFEDSLSGLKFFPGLKRKLPEDMQM 231

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRTG+ME +PVTS+KKSRFMACRPVNLEINLPS T  ENIE+VR    VGSK
Sbjct: 232 DGIVSPSDPRTGAMEFTPVTSVKKSRFMACRPVNLEINLPSCTAAENIEVVRHSSTVGSK 291

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 292 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 325

[Close]

BLAST of HG10022071 vs. ExPASy TrEMBL
Match: A0A0A0KU82 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G636560 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.7e-109
Identity = 197/214 (92.06%), Postives = 201/214 (93.93%), Query Frame = 0


Query: 1   MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 60
           MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW
Sbjct: 112 MAVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 171

Query: 61  PVLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQM 120
           PVLIDDFVEHMYRRSGSNKVSKL CNCGDSE +SGVFEDSLSGLKFFPGLKRKLPEDMQM
Sbjct: 172 PVLIDDFVEHMYRRSGSNKVSKLSCNCGDSE-ESGVFEDSLSGLKFFPGLKRKLPEDMQM 231

Query: 121 DGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSK 180
           DGIVS SDPRT +ME +PVTS+KKSRFMACRPVNLEIN PS T  ENIEMVR    VGSK
Sbjct: 232 DGIVSPSDPRTSAMEFTPVTSVKKSRFMACRPVNLEINSPSCTAAENIEMVRHNSTVGSK 291

Query: 181 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT
Sbjct: 292 SPCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 324

[Close]

BLAST of HG10022071 vs. ExPASy TrEMBL
Match: A0A6J1FFS5 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111445042 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 185/212 (87.26%), Postives = 194/212 (91.51%), Query Frame = 0


Query: 2   AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWP 61
           AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWP
Sbjct: 114 AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWP 173

Query: 62  VLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQMD 121
           VLIDDFVEHMYRRSGSNK+SKL C CGDSESQSGVFEDSL GLK FPGLKRKLPEDMQ+D
Sbjct: 174 VLIDDFVEHMYRRSGSNKISKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKLPEDMQVD 233

Query: 122 GIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSKS 181
            I+SLSDPRTGSMEL PVTS+KK+RFM CRPVNLE+NLPS T DENI+MVR   AVG KS
Sbjct: 234 DIMSLSDPRTGSMELIPVTSLKKTRFMGCRPVNLEVNLPSCTADENIDMVRHNGAVGCKS 293

Query: 182 PCAVEGCLSKGFAGLFSTRLFLGLDQERKASF 211
           PCA+EG LSKGFAGLFSTR  LGLD+ERKASF
Sbjct: 294 PCAIEGFLSKGFAGLFSTRSILGLDRERKASF 325

[Close]

BLAST of HG10022071 vs. ExPASy TrEMBL
Match: A0A6J1JZZ8 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489407 PE=4 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 185/213 (86.85%), Postives = 192/213 (90.14%), Query Frame = 0


Query: 2   AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWP 61
           AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTW QVLAFS CVHENLEGYDPEGAWP
Sbjct: 165 AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWLQVLAFSCCVHENLEGYDPEGAWP 224

Query: 62  VLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDSLSGLKFFPGLKRKLPEDMQMD 121
           VLIDDFVEHMYRRSGSNK+ KL C CGDSESQSGVFEDSL GLK FPGLKRK PEDMQ+D
Sbjct: 225 VLIDDFVEHMYRRSGSNKIPKLYCTCGDSESQSGVFEDSLPGLKSFPGLKRKFPEDMQVD 284

Query: 122 GIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIEMVR---AVGSKS 181
            I+S SDPRTGSMEL PVTS+KKSRFM CRPVNLE+NLPS T DENI+MVR   AVG KS
Sbjct: 285 DIMSPSDPRTGSMELIPVTSVKKSRFMGCRPVNLEVNLPSCTADENIDMVRYNGAVGCKS 344

Query: 182 PCAVEGCLSKGFAGLFSTRLFLGLDQERKASFT 212
           PCA+EGCLSKGFAGLFST   LGLDQERKASFT
Sbjct: 345 PCAIEGCLSKGFAGLFSTCSILGLDQERKASFT 377

[Close]

BLAST of HG10022071 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 191.0 bits (484), Expect = 9.4e-49
Identity = 103/207 (49.76%), Postives = 126/207 (60.87%), Query Frame = 0


Query: 2   AVRAWRLVLDGRFRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAWP 61
           A+ AW+LVL GRFRLLN+WCDF+E NQRHNISEDTWQQVLAFSRCVHENLEGYD EGAWP
Sbjct: 110 AITAWKLVLAGRFRLLNRWCDFIEKNQRHNISEDTWQQVLAFSRCVHENLEGYDSEGAWP 169

Query: 62  VLIDDFVEHMYRRSGSNKVSKLCCNCGDSESQSGVFEDS---------LSGLKFFPGLKR 121
           VLIDDFVEHMY   G NK + L C CGD+ES+S ++++           +GL+  PGLKR
Sbjct: 170 VLIDDFVEHMYSILGPNKDTSLFCKCGDTESESCLYQEDEHHKDYRRPHTGLRNIPGLKR 229

Query: 122 KLP---EDMQMDGIVSLSDPRTGSMELSPVTSIKKSRFMACRPVNLEINLPSYTVDENIE 181
           K     ++ + D    + + +  S  L+    IK S    C                   
Sbjct: 230 KTSKKNDEEEEDEDEEVLETQNSSSLLN-FKRIKTSNSPRC------------------- 289

Query: 182 MVRAVGSKSPCAVEGCLSKGFAGLFST 197
                 SKSPC++E  LS+GFA L ST
Sbjct: 290 -----SSKSPCSIERSLSQGFASLLST 291

[Close]

BLAST of HG10022071 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 81.6 bits (200), Expect = 8.1e-16
Identity = 35/75 (46.67%), Postives = 50/75 (66.67%), Query Frame = 0


Query: 2   AVRAWRLVLDGR-FRLLNQWCDFVENNQRHNISEDTWQQVLAFSRCVHENLEGYDPEGAW 61
           A+  W+L+   R + L+  WCDF+++     IS+DTW Q+L FSR V   L  YD EGAW
Sbjct: 172 AIGMWQLLFAEREWPLVTHWCDFLQDRHNKAISKDTWAQLLEFSRMVDPVLSNYDAEGAW 231

Query: 62  PVLIDDFVEHMYRRS 76
           P LID+FVE++Y ++
Sbjct: 232 PYLIDEFVEYLYDKN 246

The following BLAST results are available for this feature:

BLAST of HG10022071 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890437.1	5.1e-113	93.93	uncharacterized protein LOC120080000 isoform X1 [Benincasa hispida]	[more]
XP_038890438.1	5.1e-113	93.93	uncharacterized protein LOC120080000 isoform X2 [Benincasa hispida]	[more]
KAA0039495.1	4.3e-112	92.99	defective in cullin neddylation protein 1 [Cucumis melo var. makuwa] >TYK15249.1...	[more]
XP_008459316.1	4.3e-112	92.99	PREDICTED: LOW QUALITY PROTEIN: defective in cullin neddylation protein 1 [Cucum...	[more]
XP_004148700.1	7.6e-109	92.06	uncharacterized protein LOC101214663 [Cucumis sativus] >KGN52464.1 hypothetical ...	[more]

BLAST of HG10022071 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG8	1.3e-47	49.76	Defective in cullin neddylation protein AAR3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9VUQ8	4.6e-16	50.72	DCN1-like protein OS=Drosophila melanogaster OX=7227 GN=CG7427 PE=2 SV=2	[more]
Q8BZJ7	3.0e-15	50.00	DCN1-like protein 2 OS=Mus musculus OX=10090 GN=Dcun1d2 PE=1 SV=3	[more]
Q6PH85	3.9e-15	48.57	DCN1-like protein 2 OS=Homo sapiens OX=9606 GN=DCUN1D2 PE=1 SV=1	[more]
Q5ZKU1	5.1e-15	49.28	DCN1-like protein 1 OS=Gallus gallus OX=9031 GN=DCUN1D1 PE=2 SV=1	[more]

BLAST of HG10022071 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TDI6	2.1e-112	92.99	Defective in cullin neddylation protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CB35	2.1e-112	92.99	Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103498486 ...	[more]
A0A0A0KU82	3.7e-109	92.06	Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_5G6365...	[more]
A0A6J1FFS5	3.0e-103	87.26	Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JZZ8	3.0e-103	86.85	Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]

BLAST of HG10022071 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G28970.1	9.4e-49	49.76	Domain of unknown function (DUF298)	[more]
AT3G12760.1	8.1e-16	46.67	CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042460	DCN1-like, PONY binding domain	GENE3D	1.10.238.200	Cullin, PONY binding domain	coord: 1..82 e-value: 9.0E-27 score: 95.3
IPR005176	Potentiating neddylation domain	PFAM	PF03556	Cullin_binding	coord: 1..70 e-value: 1.6E-20 score: 73.8
IPR005176	Potentiating neddylation domain	PROSITE	PS51229	DCUN1	coord: 1..72 score: 24.355858
IPR014764	Defective-in-cullin neddylation protein	PANTHER	PTHR12281	RP42 RELATED	coord: 1..103
None	No IPR available	PANTHER	PTHR12281:SF31	DEFECTIVE IN CULLIN NEDDYLATION PROTEIN	coord: 1..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10022071.1	HG10022071.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051443	positive regulation of ubiquitin-protein transferase activity
biological_process	GO:0045116	protein neddylation
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0097602	cullin family protein binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0032182	ubiquitin-like protein binding