HG10021971 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021971 vs. NCBI nr
Match: XP_038893506.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 706.8 bits (1823), Expect = 9.6e-200
Identity = 352/389 (90.49%), Postives = 359/389 (92.29%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK+EAIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDEAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASD TSYVWLDEEKNHS+VLQVIVDAEMRFRDILTGLPGKMSDWLV QSSNFHKLC
Sbjct: 181 MCLPASDSTSYVWLDEEKNHSMVLQVIVDAEMRFRDILTGLPGKMSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGK+ STSKAEFNKRHKETRL
Sbjct: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKEHSTSKAEFNKRHKETRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDET-EDGDVPLSIE 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGD+T EDG VPLSIE
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDDTEEDGGVPLSIE 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           HDVDYKQQVCDVFD KGAYLRD+LSLLFI
Sbjct: 361 HDVDYKQQVCDVFDPKGAYLRDRLSLLFI 389

BLAST of HG10021971 vs. NCBI nr
Match: XP_004149039.1 (protein ALP1-like [Cucumis sativus] >KGN65671.1 hypothetical protein Csa_019843 [Cucumis sativus])

HSP 1 Score: 705.7 bits (1820), Expect = 2.1e-199
Identity = 346/388 (89.18%), Postives = 359/388 (92.53%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLD NGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LN--------------------HYLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LN                    H+LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTSYVWLD++KNHS+VLQVIVDAEMRFRDILTGLPGK+SDWLV QSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKR EL DRSEIREYIIGDSGYPLLPYLVTPYDGK+LSTSK EFNKRHKETRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAY+RD+LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of HG10021971 vs. NCBI nr
Match: TYK01291.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 698.7 bits (1802), Expect = 2.6e-197
Identity = 345/388 (88.92%), Postives = 358/388 (92.27%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD+EKNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. NCBI nr
Match: KAA0033909.1 (putative nuclease HARBI1 [Cucumis melo var. makuwa])

HSP 1 Score: 698.7 bits (1802), Expect = 2.6e-197
Identity = 345/388 (88.92%), Postives = 358/388 (92.27%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD+EKNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. NCBI nr
Match: XP_008457540.1 (PREDICTED: LOW QUALITY PROTEIN: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 695.7 bits (1794), Expect = 2.2e-196
Identity = 344/388 (88.66%), Postives = 356/388 (91.75%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD  KNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.8e-115
Identity = 210/403 (52.11%), Postives = 277/403 (68.73%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN----GTAS---------------DSSEKEEAIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N     TA+               D     +++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSTFKVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S FK+SRKTFDYIC LVK D TAK  NF+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLN--------------------HYLHWPSTEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N                    H+L WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN S+ LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDG 300
           PG ++D +V ++S F+KL +KG+RLNG++L L++R+E+REYI+GDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH E     Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLS 365
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LRD+LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of HG10021971 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 5.1e-79
Identity = 167/396 (42.17%), Postives = 233/396 (58.84%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKE---------EAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ LD     + + EK+         EAI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSTFKVSRKTFDYICLLVKDDMTAK-SGNFTFLNGRPLSLCDQVAVALRRLGS 120
           S     FK  F+ S+ TF YIC LV++D+ ++       + GR LS+  QVA+ALRRL S
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLAS 120

Query: 121 GESLVTIGDSLGL--------------------NHYLHWPSTEVEMAQVKSKFEKIQGLP 180
           G+S V++G + G+                     H+L WP ++  + ++KSKFE++ GLP
Sbjct: 121 GDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGLP 180

Query: 181 NCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDW 240
           NCCG+IDTTHI M LPA    S  W D+EKN+S+ LQ + D EMRF +++TG PG M+  
Sbjct: 181 NCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVS 240

Query: 241 LVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSK 300
            + + S F KLC+  + L+G    L+  ++IREY++G   YPLLP+L+TP+D    S S 
Sbjct: 241 KLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSM 300

Query: 301 AEFNKRHKETRLVVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDE 360
             FN+RH++ R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD 
Sbjct: 301 VAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 360

Query: 361 TEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLS 365
            ++ DVPLS  HD  Y  + C   +  G+ LR  L+
Sbjct: 361 LQE-DVPLSGHHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of HG10021971 vs. ExPASy Swiss-Prot
Match: B0BN95 (Putative nuclease HARBI1 OS=Rattus norvegicus OX=10116 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.7e-19
Identity = 71/299 (23.75%), Postives = 132/299 (44.15%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGL--------------------NHYLHWPST 150
           R +S   Q+  AL    SG     +GD++G+                    + ++H+P+ 
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDA 210
           E  +  +K +F  + G+P   G++D  H+ +  P ++  SYV  + +  HS+   V+ D 
Sbjct: 125 EAAIQSLKDEFYGLAGMPGVIGAVDCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYP 270
                 + T  PG + D  V Q S+     + G   +              +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQQSSLSSQFETGMPKD-------------SWLLGDSSFF 244

Query: 271 LLPYLVTPYDGKKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQG----VMWRPDKH 330
           L  +L+TP    + + ++  +N+ H  T  V++  L  L  R+R + G    + + P+K 
Sbjct: 245 LHTWLLTPLHIPE-TPAEYRYNRAHSATHSVIEKTLRTLCCRFRCLDGSKGALQYSPEKS 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLSL 366
               IIL CC+LHNI ++ G +     V   IE   + + +  +  D +   +R +L L
Sbjct: 305 --SHIILACCVLHNISLEHGMDVWSSPVTGPIEQPPEGEDEQMESLDLEADRIRQELIL 345

BLAST of HG10021971 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.8e-19
Identity = 68/299 (22.74%), Postives = 129/299 (43.14%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGL--------------------NHYLHWPST 150
           R +S   Q+  AL    SG     +GD++G+                    + ++H+P+ 
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDA 210
           E  +  +K +F  + G+P   G +D  H+ +  P ++  SYV  + +  HS+   ++ D 
Sbjct: 125 EASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLSYV--NRKGLHSLNCLMVCDI 184

Query: 211 EMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYP 270
                 + T  PG + D +V Q S+     + G                  +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCVVLQQSSLSSQFEAG-------------MHKESWLLGDSSFF 244

Query: 271 LLPYLVTPYDGKKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQG----VMWRPDKH 330
           L  +L+TP    + + ++  +N  H  T  V++     L  R+R + G    + + P+K 
Sbjct: 245 LRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKS 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLSL 366
               IIL CC+LHNI ++ G +     V   +E   + + +  +  D +   +R +L L
Sbjct: 305 --SHIILACCVLHNISLEHGMDVWSSPVTGPVEQPPEEEYEHMESLDLEADRIRQELML 345

BLAST of HG10021971 vs. ExPASy Swiss-Prot
Match: Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.8e-19
Identity = 72/299 (24.08%), Postives = 130/299 (43.48%), Query Frame = 0

Query: 91  RPLSLCDQVAVALRRLGSGESLVTIGDSLGL--------------------NHYLHWPST 150
           R +S   Q+  AL    SG     +GD++G+                    + ++H+P  
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPVD 124

Query: 151 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDA 210
           E  +  +K +F  + G+P   G  D  H+ +  P ++  SYV  + +  HS+   V+ D 
Sbjct: 125 EAAVQSLKDEFYGLAGMPGVIGVADCIHVAIKAPNAEDLSYV--NRKGLHSLNCLVVCDI 184

Query: 211 EMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYP 270
                 + T  PG + D  V Q S+     + G   +              +++GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCAVLQRSSLTSQFETGMPKD-------------SWLLGDSSFF 244

Query: 271 LLPYLVTPYDGKKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQG----VMWRPDKH 330
           L  +L+TP    + + ++  +N+ H  T  V++  L  L  R+R + G    + + P+K 
Sbjct: 245 LRSWLLTPLPIPE-TAAEYRYNRAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEK- 304

Query: 331 RLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLSL 366
               IIL CC+LHNI +D G +     VP  I+   + + +  +  D +   +R +L L
Sbjct: 305 -CSHIILACCVLHNISLDHGMDVWSSPVPGPIDQPPEGEDEHMESLDLEADRIRQELIL 345

BLAST of HG10021971 vs. ExPASy TrEMBL
Match: A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 1.0e-199
Identity = 346/388 (89.18%), Postives = 359/388 (92.53%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLD NGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDCNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LN--------------------HYLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LN                    H+LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTSYVWLD++KNHS+VLQVIVDAEMRFRDILTGLPGK+SDWLV QSSNFHKLC
Sbjct: 181 MCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKR EL DRSEIREYIIGDSGYPLLPYLVTPYDGK+LSTSK EFNKRHKETRL
Sbjct: 241 DKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETE+G+VPLSIEH
Sbjct: 301 VVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAY+RD+LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of HG10021971 vs. ExPASy TrEMBL
Match: A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.3e-197
Identity = 345/388 (88.92%), Postives = 358/388 (92.27%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD+EKNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. ExPASy TrEMBL
Match: A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.3e-197
Identity = 345/388 (88.92%), Postives = 358/388 (92.27%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD+EKNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. ExPASy TrEMBL
Match: A0A1S3C6F2 (LOW QUALITY PROTEIN: putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103497206 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.1e-196
Identity = 344/388 (88.66%), Postives = 356/388 (91.75%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKSTF 60
           MGPIRGLRKKKKLERKLDSNGTASDSSEK++AIDWWDDFSKRTNGLHSASKGLDRFKS F
Sbjct: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSIF 60

Query: 61  KVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120
           KVSRKTFDYICLLVKDDMTAKSG+FTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG
Sbjct: 61  KVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSLG 120

Query: 121 LNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180
           LNH                    +LHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHIT
Sbjct: 121 LNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHIT 180

Query: 181 MCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLC 240
           MCLPASDPTS+VWLD  KNHS+VLQVIVDAEMRFRDILTGLPGK+SD LV QSSNFHKLC
Sbjct: 181 MCLPASDPTSFVWLDXRKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKLC 240

Query: 241 DKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRL 300
           DKGERLNGKRLEL DRSEI+EYI+GDSGYPLL YLVTPYDGK+LSTSKAEFNKRH  TRL
Sbjct: 241 DKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATRL 300

Query: 301 VVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360
           VVQ AL MLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH
Sbjct: 301 VVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIEH 360

Query: 361 DVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           DVDYKQQVCDVFDSKGAYLR++LSLLFI
Sbjct: 361 DVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of HG10021971 vs. ExPASy TrEMBL
Match: A0A6J1GBJ6 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 7.4e-182
Identity = 320/389 (82.26%), Postives = 340/389 (87.40%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN-GTASDSSEKEEAIDWWDDFSKRTNGLHSASKGLDRFKST 60
           MGPIRG RKKKKLERKLD+N  TASDSSEK++A+DWWDDFS+RT GLHS  +GLD FKS 
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120
           FKVSRKTFDYICLLVKDDMTA+S NFTFLNGRPLSL DQVAVALRRLGSG+SLVTIG S 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAESSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GLNH--------------------YLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           GLNH                    +LHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKL 240
           TMCLP  DPTS VWLD EKNHS+VLQVIVDAEMRFRDI+TGLPGKMSDWLV QSSNFHKL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CDKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETR 300
           C+KGERLNGKRLE  +RSEIREYIIGDSGYPLLPYLVTPYDGK+L  SKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 LVVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPLSIE 360
           LVVQ AL  LKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIID+GDE EDG+VP+S+E
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSME 360

Query: 361 HDVDYKQQVCDVFDSKGAYLRDKLSLLFI 369
           HD DYKQQ+CDV+DSKGAYLRDKLSLLFI
Sbjct: 361 HDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of HG10021971 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 415.6 bits (1067), Expect = 4.1e-116
Identity = 210/403 (52.11%), Postives = 277/403 (68.73%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSN----GTAS---------------DSSEKEEAIDWWDDFSK 60
           MGPI+ ++KKK+ E+K+D N     TA+               D     +++DWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RTNGLHSASKGLDRFKSTFKVSRKTFDYICLLVKDDMTAKSGNFTFLNGRPLSLCDQVAV 120
           R   ++  S     F+S FK+SRKTFDYIC LVK D TAK  NF+  NG PLSL D+VAV
Sbjct: 61  R---IYGGSTDPKTFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVTIGDSLGLN--------------------HYLHWPSTEVEMAQVKSKF 180
           ALRRLGSGESL  IG++ G+N                    H+L WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGL 240
           EKI GLPNCCG+ID THI M LPA +P++ VWLD EKN S+ LQ +VD +MRF D++ G 
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDG 300
           PG ++D +V ++S F+KL +KG+RLNG++L L++R+E+REYI+GDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNI 360
           K  S  + EFNKRH E     Q AL+ LK+RWRII GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 IIDIGDETEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLS 365
           IID+ D+T D D PLS +HD++Y+Q+ C + D   + LRD+LS
Sbjct: 361 IIDMEDQTLD-DQPLSQQHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of HG10021971 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 296.2 bits (757), Expect = 3.6e-80
Identity = 167/396 (42.17%), Postives = 233/396 (58.84%), Query Frame = 0

Query: 1   MGPIRGLRKKKKLERKLDSNGTASDSSEKE---------EAI--DWWDDFSKRTNGLHSA 60
           M P++  +KKK  ++ LD     + + EK+         EAI  DWWD F  R +     
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  SKGLDRFKSTFKVSRKTFDYICLLVKDDMTAK-SGNFTFLNGRPLSLCDQVAVALRRLGS 120
           S     FK  F+ S+ TF YIC LV++D+ ++       + GR LS+  QVA+ALRRL S
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLAS 120

Query: 121 GESLVTIGDSLGL--------------------NHYLHWPSTEVEMAQVKSKFEKIQGLP 180
           G+S V++G + G+                     H+L WP ++  + ++KSKFE++ GLP
Sbjct: 121 GDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGLP 180

Query: 181 NCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVVLQVIVDAEMRFRDILTGLPGKMSDW 240
           NCCG+IDTTHI M LPA    S  W D+EKN+S+ LQ + D EMRF +++TG PG M+  
Sbjct: 181 NCCGAIDTTHIIMTLPAVQ-ASDDWCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVS 240

Query: 241 LVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYIIGDSGYPLLPYLVTPYDGKKLSTSK 300
            + + S F KLC+  + L+G    L+  ++IREY++G   YPLLP+L+TP+D    S S 
Sbjct: 241 KLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSM 300

Query: 301 AEFNKRHKETRLVVQPALTMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDE 360
             FN+RH++ R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNIIID GD 
Sbjct: 301 VAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 360

Query: 361 TEDGDVPLSIEHDVDYKQQVCDVFDSKGAYLRDKLS 365
            ++ DVPLS  HD  Y  + C   +  G+ LR  L+
Sbjct: 361 LQE-DVPLSGHHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of HG10021971 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 125.9 bits (315), Expect = 6.5e-29
Identity = 83/319 (26.02%), Postives = 143/319 (44.83%), Query Frame = 0

Query: 29  KEEAIDWWDDFSKRTNGLHSASKGLDRFKSTFKVSRKTFDYICLLVKDDMTAKSGNFTFL 88
           K+ +  WW++ S+            + FK  F++S+ TF+ IC    D++ +        
Sbjct: 155 KDRSRAWWEECSR-------LDYPEEDFKKAFRMSKSTFELIC----DELNSAVAKEDTA 214

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLN---------------------HYLHW 148
               + +  +VAV + RL +GE L  +    GL                       YL W
Sbjct: 215 LRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW 274

Query: 149 PSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSY-----VWLDEEKNHSV 208
           P  E  +  ++ +FE + G+PN  GS+ TTHI +  P     SY        +++ ++S+
Sbjct: 275 PDDE-SLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 334

Query: 209 VLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREY 268
            +Q +V+ +  F D+  G PG M D  V + S  ++  + G  L G             +
Sbjct: 335 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM------------W 394

Query: 269 IIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQGVMWR 322
           + G  G+PLL +++ PY  + L+ ++  FN++  E + V + A   LK RW  +Q     
Sbjct: 395 VAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTE 448

BLAST of HG10021971 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 123.6 bits (309), Expect = 3.2e-28
Identity = 91/319 (28.53%), Postives = 145/319 (45.45%), Query Frame = 0

Query: 29  KEEAIDWWDDFSKRTNGLHSASKGLDRFKSTFKVSRKTFDYICLLVKDDMTAKSGNFTFL 88
           KE   DWWD  S+            D F+  F++S+ TF+ IC  +   +T K+   T L
Sbjct: 193 KERTTDWWDRVSR-------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKN---TML 252

Query: 89  NGRPLSLCDQVAVALRRLGSGESLVTIGDSLGLN---------------------HYLHW 148
               +    +V V + RL +G  L  + +  GL                       YL W
Sbjct: 253 RD-AIPAPKRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW 312

Query: 149 PSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSY-----VWLDEEKNHSV 208
           PS + E+   K+KFE +  +PN  GSI TTHI +  P     +Y        +++ ++S+
Sbjct: 313 PS-DSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 372

Query: 209 VLQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREY 268
            +Q +V+A+  F D+  G PG ++D  + + S+         R    R  L D      +
Sbjct: 373 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSL-------SRQRAARGMLRD-----SW 432

Query: 269 IIGDSGYPLLPYLVTPYDGKKLSTSKAEFNKRHKETRLVVQPALTMLKERWRIIQGVMWR 322
           I+G+SG+PL  YL+ PY  + L+ ++  FN+   E + +   A   LK RW  +Q     
Sbjct: 433 IVGNSGFPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTE 486

BLAST of HG10021971 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 95.5 bits (236), Expect = 9.4e-20
Identity = 72/259 (27.80%), Postives = 115/259 (44.40%), Query Frame = 0

Query: 87  FLNGRPLSLCDQVAVA--LRRLGSGESLVTIGDSLGLNHYL------------------- 146
           F+    LSL    AVA  L RL  G S  T+     L+ YL                   
Sbjct: 138 FITASNLSLPADYAVAMVLSRLAHGCSAKTLASRYSLDPYLISKITNMVTRLLATKLYPE 197

Query: 147 --HWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPTSYVWLDEEKNHSVV 206
               P  +  + +    FE++  LPN CG+ID+T + +          ++  +    +V+
Sbjct: 198 FIKIPVGKRRLIETTQGFEELTSLPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVL 257

Query: 207 LQVIVDAEMRFRDILTGLPGKMSDWLVSQSSNFHKLCDKGERLNGKRLELNDRSEIREYI 266
           LQV+ D +  F D+    PG   D    + S  +K    G+ +  K + +     +R YI
Sbjct: 258 LQVVADHKKIFWDVCVKAPGGEDDSSHFRDSLLYKRLTSGDIVWEKVINIRGH-HVRPYI 317

Query: 267 IGDSGYPLLPYLVTPYDGKKLSTSKAE-FNKRHKETRLVVQPALTMLKERWRIIQGVMWR 322
           +GD  YPLL +L+TP+      T     F+    + R VV  A+ +LK RW+I+Q +   
Sbjct: 318 VGDWCYPLLSFLMTPFSPNGSGTPPENLFDGMLMKGRSVVVEAIGLLKARWKILQSL--N 377