HG10021778 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021778 vs. NCBI nr
Match: XP_023551916.1 (trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_023551917.1 trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_023551918.1 trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >XP_023551919.1 trehalose-phosphate phosphatase A-like [Cucurbita pepo subsp. pepo] >KAG6576800.1 Trehalose-phosphate phosphatase A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014827.1 Trehalose-phosphate phosphatase A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.0 bits (1730), Expect = 7.0e-189
Identity = 329/366 (89.89%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTG+LDDVRAS WLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRASCWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKDVVNELPS DPD LYR+WM+KYPSA+ SF+QI+T+AKGK I LFLDYDGTLSPIVDNP
Sbjct: 78  SKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRIVLFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           DCAFMSDAMRA VKEAAKYFPTAIISGRSRDK          VYEFIGLRELYYAGSHGM
Sbjct: 138 DCAFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VYEFIGLRELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS N+QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGL+NCD
Sbjct: 258 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIYVGDD TDEDAFR LKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. NCBI nr
Match: XP_022985485.1 (trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022985486.1 trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022985487.1 trehalose-phosphate phosphatase A-like [Cucurbita maxima] >XP_022985488.1 trehalose-phosphate phosphatase A-like [Cucurbita maxima])

HSP 1 Score: 671.0 bits (1730), Expect = 7.0e-189
Identity = 329/366 (89.89%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTG+LDDVRAS WLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRASCWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKDVVNELPS DPD LYR+WM+KYPSA+ SF+QI+T+AKGK I LFLDYDGTLSPIVDNP
Sbjct: 78  SKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRIVLFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           DCAFMSDAMRA VKEAAKYFPTAIISGRSRDK          VYEFIGLRELYYAGSHGM
Sbjct: 138 DCAFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VYEFIGLRELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS N+QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITRGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGL+NCD
Sbjct: 258 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIYVGDD TDEDAFR LKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. NCBI nr
Match: XP_022923092.1 (trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022923093.1 trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022923094.1 trehalose-phosphate phosphatase A-like [Cucurbita moschata] >XP_022923095.1 trehalose-phosphate phosphatase A-like [Cucurbita moschata])

HSP 1 Score: 667.9 bits (1722), Expect = 6.0e-188
Identity = 327/366 (89.34%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTG+LDDVRAS WLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRASCWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKDVVNELPS DPD LYR+WM+KYPSA+ SF+QI+T+AKGK I LFLDYDGTLSPIVDNP
Sbjct: 78  SKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRIVLFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           DCAFMSDAMRA VKEAAKYFPTAIISGRSRDK          V+EFIGLRELYYAGSHGM
Sbjct: 138 DCAFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VFEFIGLRELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS N+QGKEVMFQPASEFLPLIDEV+KSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVFKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGL+NCD
Sbjct: 258 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIYVGDD TDEDAFR LKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. NCBI nr
Match: XP_038877832.1 (trehalose-phosphate phosphatase A [Benincasa hispida] >XP_038877836.1 trehalose-phosphate phosphatase A [Benincasa hispida])

HSP 1 Score: 662.9 bits (1709), Expect = 1.9e-186
Identity = 329/364 (90.38%), Postives = 340/364 (93.41%), Query Frame = 0

Query: 82  KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISK 141
           KSRLGVHSNMLPYA APGVGFSPNLLLIPRKKTG+LDDVRASSWLDAMKSSSPPPNRISK
Sbjct: 20  KSRLGVHSNMLPYAPAPGVGFSPNLLLIPRKKTGVLDDVRASSWLDAMKSSSPPPNRISK 79

Query: 142 DVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDC 201
           DVVNELPS DP  LY +WMIKYPSA+ASFDQI+T AKGK IALFLDYDGTLSPIVDNPDC
Sbjct: 80  DVVNELPSIDPIFLYCNWMIKYPSALASFDQIVTQAKGKRIALFLDYDGTLSPIVDNPDC 139

Query: 202 AFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDI 261
           AFMSDAMRA VKEAAKYFPTAIISGRSRDK          VYEFIGLRELYYAGSHGMDI
Sbjct: 140 AFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VYEFIGLRELYYAGSHGMDI 199

Query: 262 MVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNV 321
           MVSDRHS N+QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAK+ENNKFCVSVHYRNV
Sbjct: 200 MVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKIENNKFCVSVHYRNV 259

Query: 322 DDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEV 381
           DDKNWNALAN VYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAV FLLESLGL NCDEV
Sbjct: 260 DDKNWNALANVVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLESLGLDNCDEV 319

Query: 382 LPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 441
           LPIYVGDD TDEDAFRVLKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW+K
Sbjct: 320 LPIYVGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTWRK 373

Query: 442 SNAL 446
           S+AL
Sbjct: 380 SSAL 373

BLAST of HG10021778 vs. NCBI nr
Match: XP_004148142.1 (trehalose-phosphate phosphatase A isoform X1 [Cucumis sativus] >XP_011651115.1 trehalose-phosphate phosphatase A isoform X1 [Cucumis sativus])

HSP 1 Score: 655.2 bits (1689), Expect = 4.0e-184
Identity = 322/366 (87.98%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVH NMLPY +APGVGFSPN+LLIPRKKTG+LDDVRASSWLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHLNMLPYVSAPGVGFSPNMLLIPRKKTGVLDDVRASSWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKD +NELPS DPD+LYR+WMIKYPSA+ASFDQI+ +AKGK IALFLDYDGTLSPIVDNP
Sbjct: 78  SKDDMNELPSLDPDILYRNWMIKYPSALASFDQIVPHAKGKRIALFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           D AFMSDAMRATVKEAAKYFPTAIISGRSRDK          VYEFIGL+ELYYAGSHGM
Sbjct: 138 DGAFMSDAMRATVKEAAKYFPTAIISGRSRDK----------VYEFIGLKELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHSA++QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSADNQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAV FLLE+LGL+NCD
Sbjct: 258 NVDDKNWNALANSVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLETLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIY+GDD TDEDAFRVLKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYIGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. ExPASy Swiss-Prot
Match: O64896 (Trehalose-phosphate phosphatase A OS=Arabidopsis thaliana OX=3702 GN=TPPA PE=1 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.6e-133
Identity = 242/379 (63.85%), Postives = 294/379 (77.57%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPN---LLLIPRKKTGLLDDVRASSWLDAMKSSSPPP 139
           +  SRL +  N LPY++A     S N   LL +PRKKTG+LDDV+++ WLDAMKSSSPPP
Sbjct: 18  ISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDDVKSNGWLDAMKSSSPPP 77

Query: 140 NRISKDVVNELPSPDPDLLYRSWM-IKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPI 199
             ++KD    L +   D+ YR WM +KYPSA+ SF++I+++AKGK IALFLDYDGTLSPI
Sbjct: 78  TILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPI 137

Query: 200 VDNPDCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAG 259
           V+ PDCA+MS AMR+ V+  AKYFPTAIISGRSRDK          VYEF+ L ELYYAG
Sbjct: 138 VEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDK----------VYEFVNLSELYYAG 197

Query: 260 SHGMDIMVSDRHSAN----------DQGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAG 319
           SHGMDIM     S N          +QGK+V +FQPASEFLP+ID+V  SLIE T+ I G
Sbjct: 198 SHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIESTKDIKG 257

Query: 320 AKVENNKFCVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGK 379
            KVE+NKFC+SVHYRNV++KNW  +A CV D++  YP+LR++HGRKVLE+RPVI WDKGK
Sbjct: 258 VKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPVIDWDKGK 317

Query: 380 AVTFLLESLGLSNCDEVLPIYVGDDCTDEDAFRVLKE-RNCGFGILVSSVTKDSSAAYSL 439
           AVTFLLESLGL+NC++VLPIYVGDD TDEDAF+VL++  N G+GILVS+V KDS+A YSL
Sbjct: 318 AVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKDSNAFYSL 377

Query: 440 RDPSEVMEFLKSLVTWKKS 443
           RDPSEVMEFLKSLVTWK+S
Sbjct: 378 RDPSEVMEFLKSLVTWKRS 383

BLAST of HG10021778 vs. ExPASy Swiss-Prot
Match: Q9SUW0 (Probable trehalose-phosphate phosphatase G OS=Arabidopsis thaliana OX=3702 GN=TPPG PE=2 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 6.1e-119
Identity = 224/351 (63.82%), Postives = 266/351 (75.78%), Query Frame = 0

Query: 103 SPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISKDVVNELPSPDPDLLYRSWMIK 162
           S   ++  RKK   LDDVR++ WLDAM SSSPP  R+ KD   E+ +P+ D   R+WM+K
Sbjct: 30  SGRFMMNSRKKIPKLDDVRSNGWLDAMISSSPPRKRLVKDFNIEI-APEDDFSQRAWMLK 89

Query: 163 YPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDCAFMSDAMRATVKEAAKYFPTA 222
           YPSA+ SF  I   AK K IA+FLDYDGTLSPIVD+PD A MSDAMRA VK+ AKYFPTA
Sbjct: 90  YPSAITSFAHIAAQAKNKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRAAVKDVAKYFPTA 149

Query: 223 IISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDIM--VSDRHSAND--------- 282
           IISGRSRDK          VY+ +GL ELYYAGSHGMDIM  V+   S  D         
Sbjct: 150 IISGRSRDK----------VYQLVGLTELYYAGSHGMDIMTPVNPNGSPEDPNCIKTTDQ 209

Query: 283 QGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNVDDKNWNALA 342
           QG+EV +FQPA EF+P+I+EVY +L+EIT+ I GAKVEN+KFC SVHYRNVD+K+W  +A
Sbjct: 210 QGEEVNLFQPAKEFIPVIEEVYNNLVEITKCIKGAKVENHKFCTSVHYRNVDEKDWPLVA 269

Query: 343 NCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEVLPIYVGDDC 402
             V+D L+ YPRLR++HGRKVLEVRPVI W+KGKAV FLLESLGLSN DE LPI++GDD 
Sbjct: 270 QRVHDHLKRYPRLRITHGRKVLEVRPVIEWNKGKAVEFLLESLGLSNNDEFLPIFIGDDK 329

Query: 403 TDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 442
           TDEDAF+VL+E N GFGILVSSV K+S+A YSLRDPSEV +FLK+LV W K
Sbjct: 330 TDEDAFKVLREGNRGFGILVSSVPKESNAFYSLRDPSEVKKFLKTLVKWGK 369

BLAST of HG10021778 vs. ExPASy Swiss-Prot
Match: Q9SU39 (Probable trehalose-phosphate phosphatase F OS=Arabidopsis thaliana OX=3702 GN=TPPF PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.4e-118
Identity = 216/348 (62.07%), Postives = 266/348 (76.44%), Query Frame = 0

Query: 100 VGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISKDVVNELPSPDPDLLYRSW 159
           +G S   ++   KK   LDDVR++ WLDAM SSSPP  ++ KD   E+ +P+ D   R+W
Sbjct: 24  LGVSSRFMMSQWKKPAKLDDVRSNGWLDAMISSSPPRKKLVKDFNVEV-APEDDFAQRAW 83

Query: 160 MIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDCAFMSDAMRATVKEAAKYF 219
           M+KYPSA++SF  I   AK K IA+FLDYDGTLSPIVD+PD A MSDAMR+ VK+ A YF
Sbjct: 84  MVKYPSAISSFAHIAAQAKKKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRSAVKDVASYF 143

Query: 220 PTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDIMVSDR-----HSANDQGK 279
           PTAIISGRSRDK          VY+ +GL ELYYAGSHGMDIM S        S + QGK
Sbjct: 144 PTAIISGRSRDK----------VYQLVGLTELYYAGSHGMDIMTSSDGPNCFKSTDQQGK 203

Query: 280 EV-MFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNVDDKNWNALANCV 339
           EV +FQPA EF+P+IDEV+++L+E  + I GAKVEN+KFC SVHYRNVD+K+W  +A  V
Sbjct: 204 EVNLFQPAREFIPVIDEVFRTLVEKMKDIKGAKVENHKFCASVHYRNVDEKDWPIIAQRV 263

Query: 340 YDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEVLPIYVGDDCTDE 399
           +D L+ YPRLR++HGRKVLEVRPVI W+KG+AV FLLESLGLSN D++LPIY+GDD TDE
Sbjct: 264 HDHLKQYPRLRLTHGRKVLEVRPVIDWNKGRAVEFLLESLGLSNKDDLLPIYIGDDTTDE 323

Query: 400 DAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 442
           DAF+VL++ N GFGILVSS+ K+S+A YSLRDPSEV +FLK+LV W K
Sbjct: 324 DAFKVLRDGNRGFGILVSSIPKESNAFYSLRDPSEVKKFLKTLVKWAK 360

BLAST of HG10021778 vs. ExPASy Swiss-Prot
Match: Q75WV3 (Probable trehalose-phosphate phosphatase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP1 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.8e-110
Identity = 205/359 (57.10%), Postives = 264/359 (73.54%), Query Frame = 0

Query: 86  GVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISKDVVN 145
           G+ SN++ ++  PG G+S + + +   +   +++V  +  LDAMKSSS P  R++     
Sbjct: 23  GLPSNLMQFSVMPG-GYSSSGMNVGVSRL-KIEEVLVNGLLDAMKSSS-PRRRLNVAFGE 82

Query: 146 ELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDCAFMS 205
           +  S + D  Y +WM K PSA+ASF QI+  A+GK IA+FLDYDGTLSPIVD+PD A MS
Sbjct: 83  DNSSEEEDPAYSAWMAKCPSALASFKQIVASAQGKKIAVFLDYDGTLSPIVDDPDKAVMS 142

Query: 206 DAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDIM--- 265
             MRA V+  AKYFPTAI+SGRSR+K          V+EF+ L+ELYYAGSHGMDIM   
Sbjct: 143 PVMRAAVRNVAKYFPTAIVSGRSRNK----------VFEFVKLKELYYAGSHGMDIMAPS 202

Query: 266 VSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNVD 325
            +  HSA    +  +FQPA +FLP+IDEV KSL+++  GI GA VENNKFCVSVHYRNV 
Sbjct: 203 ANHEHSAEKSKQANLFQPAHDFLPMIDEVTKSLLQVVSGIEGATVENNKFCVSVHYRNVA 262

Query: 326 DKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEVL 385
           +K+W  +A  V ++LE +PRL+V++GR VLEVRPVI WDKGKAV FLL+SLGL++ + V+
Sbjct: 263 EKDWKLVARLVNEVLEAFPRLKVTNGRMVLEVRPVIDWDKGKAVEFLLQSLGLNDSENVI 322

Query: 386 PIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 442
           PIY+GDD TDEDAF+VL++RNCG+GILVS V K++ A YSLRDPSEVMEFL  LV WKK
Sbjct: 323 PIYIGDDRTDEDAFKVLRQRNCGYGILVSQVPKETEAFYSLRDPSEVMEFLNFLVRWKK 368

BLAST of HG10021778 vs. ExPASy Swiss-Prot
Match: Q9FWQ2 (Probable trehalose-phosphate phosphatase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP2 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 4.0e-110
Identity = 209/375 (55.73%), Postives = 267/375 (71.20%), Query Frame = 0

Query: 84  RLGVHSNMLPYAAAPGVGFSPNLLLIPRKK--TGLLDDVRASSWLDAMKSSSPPPNRISK 143
           +L + S ++ Y        +   L  P+KK   G +++VRA+ WLD M +SSPP  R +K
Sbjct: 17  KLALPSAVMTYTTPTSFPSTGLYLNTPKKKPLPGKIEEVRAAGWLDLMLASSPPRKRQTK 76

Query: 144 DVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDC 203
           D  N++ + + DLLYR+W++ +PSA+ SF+ I+  A+GK +ALFLDYDGTLSPIVDNP+ 
Sbjct: 77  DFANDVQADELDLLYRNWVVNHPSALTSFEDIVNLARGKRLALFLDYDGTLSPIVDNPEN 136

Query: 204 AFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDI 263
           A MSD MR+ VK  A  FPTAIISGRSRDK          V++F+ L ELYYAGSHGMDI
Sbjct: 137 AVMSDEMRSAVKHVASLFPTAIISGRSRDK----------VFDFVKLTELYYAGSHGMDI 196

Query: 264 M-------VSDRH-----SANDQGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAGAKVE 323
           M        S +H     S + +GKEV +FQPASEFLP+I EVYK L E  + I GA++E
Sbjct: 197 MGPVRKSDSSGQHVECIRSTDSEGKEVNLFQPASEFLPMISEVYKKLSESIKDIDGARME 256

Query: 324 NNKFCVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTF 383
           +NKFCVSVHYRNV   ++  +   V  +L++YP LR++HGRKVLEVRPVI W+KGKAV F
Sbjct: 257 DNKFCVSVHYRNVAPHDYGEVHQRVTAVLKNYPCLRLTHGRKVLEVRPVIDWNKGKAVEF 316

Query: 384 LLESLGLSNCDEVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSE 443
           LLESLGL   ++VLPIYVGDD TDEDAF+VLK  + GFGILVSSV KD+ A YS+RDP+E
Sbjct: 317 LLESLGLCGKEDVLPIYVGDDKTDEDAFKVLKANSIGFGILVSSVPKDTDAFYSVRDPAE 376

BLAST of HG10021778 vs. ExPASy TrEMBL
Match: A0A6J1J8A8 (Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111483480 PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 3.4e-189
Identity = 329/366 (89.89%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTG+LDDVRAS WLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRASCWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKDVVNELPS DPD LYR+WM+KYPSA+ SF+QI+T+AKGK I LFLDYDGTLSPIVDNP
Sbjct: 78  SKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRIVLFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           DCAFMSDAMRA VKEAAKYFPTAIISGRSRDK          VYEFIGLRELYYAGSHGM
Sbjct: 138 DCAFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VYEFIGLRELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS N+QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVYKSLIEITRGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGL+NCD
Sbjct: 258 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIYVGDD TDEDAFR LKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. ExPASy TrEMBL
Match: A0A6J1E8M3 (Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430866 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.9e-188
Identity = 327/366 (89.34%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTG+LDDVRAS WLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGVLDDVRASCWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKDVVNELPS DPD LYR+WM+KYPSA+ SF+QI+T+AKGK I LFLDYDGTLSPIVDNP
Sbjct: 78  SKDVVNELPSFDPDFLYRNWMVKYPSALGSFEQIVTHAKGKRIVLFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           DCAFMSDAMRA VKEAAKYFPTAIISGRSRDK          V+EFIGLRELYYAGSHGM
Sbjct: 138 DCAFMSDAMRAAVKEAAKYFPTAIISGRSRDK----------VFEFIGLRELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS N+QGKEVMFQPASEFLPLIDEV+KSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTNNQGKEVMFQPASEFLPLIDEVFKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGL+NCD
Sbjct: 258 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIYVGDD TDEDAFR LKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYVGDDRTDEDAFRALKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. ExPASy TrEMBL
Match: A0A0A0L662 (Trehalose 6-phosphate phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G175690 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.9e-184
Identity = 322/366 (87.98%), Postives = 344/366 (93.99%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVH NMLPY +APGVGFSPN+LLIPRKKTG+LDDVRASSWLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHLNMLPYVSAPGVGFSPNMLLIPRKKTGVLDDVRASSWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKD +NELPS DPD+LYR+WMIKYPSA+ASFDQI+ +AKGK IALFLDYDGTLSPIVDNP
Sbjct: 78  SKDDMNELPSLDPDILYRNWMIKYPSALASFDQIVPHAKGKRIALFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           D AFMSDAMRATVKEAAKYFPTAIISGRSRDK          VYEFIGL+ELYYAGSHGM
Sbjct: 138 DGAFMSDAMRATVKEAAKYFPTAIISGRSRDK----------VYEFIGLKELYYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHSA++QGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSADNQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALAN VYDLLE+YPRLRVSHGRKVLEVRPVISWDKGKAV FLLE+LGL+NCD
Sbjct: 258 NVDDKNWNALANSVYDLLENYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLETLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIY+GDD TDEDAFRVLKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYIGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. ExPASy TrEMBL
Match: A0A1S3AYK7 (Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103483982 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.4e-184
Identity = 323/366 (88.25%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVH NMLPYA+APGVGFSPN+LLIPRKKTG+LDDVRASSWLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHLNMLPYASAPGVGFSPNMLLIPRKKTGVLDDVRASSWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKD VNEL S DPD LYR+WMIKYPSA+ASFDQI+ +AKGK IALFLDYDGTLSPIVDNP
Sbjct: 78  SKDDVNELSSLDPDFLYRNWMIKYPSALASFDQIVPHAKGKRIALFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           D AFMSDAMRATVKEAAKYFPTAIISGRSRDK          VYEFIGL EL+YAGSHGM
Sbjct: 138 DGAFMSDAMRATVKEAAKYFPTAIISGRSRDK----------VYEFIGLSELHYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS +DQGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTDDQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALAN VYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAV FLLE+LGL+NCD
Sbjct: 258 NVDDKNWNALANSVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLETLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIY+GDD TDEDAFRVLKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYIGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. ExPASy TrEMBL
Match: A0A5D3DJD4 (Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00380 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.4e-184
Identity = 323/366 (88.25%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRI 139
           + KSRLGVH NMLPYA+APGVGFSPN+LLIPRKKTG+LDDVRASSWLDAMKSSSPPPNRI
Sbjct: 18  ISKSRLGVHLNMLPYASAPGVGFSPNMLLIPRKKTGVLDDVRASSWLDAMKSSSPPPNRI 77

Query: 140 SKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNP 199
           SKD VNEL S DPD LYR+WMIKYPSA+ASFDQI+ +AKGK IALFLDYDGTLSPIVDNP
Sbjct: 78  SKDDVNELSSLDPDFLYRNWMIKYPSALASFDQIVPHAKGKRIALFLDYDGTLSPIVDNP 137

Query: 200 DCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGM 259
           D AFMSDAMRATVKEAAKYFPTAIISGRSRDK          VYEFIGL EL+YAGSHGM
Sbjct: 138 DGAFMSDAMRATVKEAAKYFPTAIISGRSRDK----------VYEFIGLSELHYAGSHGM 197

Query: 260 DIMVSDRHSANDQGKEVMFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYR 319
           DIMVSDRHS +DQGKEVMFQPASEFLPLIDEVYKSLIEIT+GIAGAKVENNKFCVSVHYR
Sbjct: 198 DIMVSDRHSTDDQGKEVMFQPASEFLPLIDEVYKSLIEITKGIAGAKVENNKFCVSVHYR 257

Query: 320 NVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCD 379
           NVDDKNWNALAN VYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAV FLLE+LGL+NCD
Sbjct: 258 NVDDKNWNALANSVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVAFLLETLGLNNCD 317

Query: 380 EVLPIYVGDDCTDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTW 439
           EVLPIY+GDD TDEDAFRVLKERNCG+GILVSSV K+SSAAYSLRDPSEVMEFLKSLVTW
Sbjct: 318 EVLPIYIGDDRTDEDAFRVLKERNCGYGILVSSVAKESSAAYSLRDPSEVMEFLKSLVTW 373

Query: 440 KKSNAL 446
           +KS+AL
Sbjct: 378 RKSSAL 373

BLAST of HG10021778 vs. TAIR 10
Match: AT5G51460.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 481.5 bits (1238), Expect = 7.4e-136
Identity = 242/378 (64.02%), Postives = 294/378 (77.78%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPN---LLLIPRKKTGLLDDVRASSWLDAMKSSSPPP 139
           +  SRL +  N LPY++A     S N   LL +PRKKTG+LDDV+++ WLDAMKSSSPPP
Sbjct: 18  ISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDDVKSNGWLDAMKSSSPPP 77

Query: 140 NRISKDVVNELPSPDPDLLYRSWMIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIV 199
             ++KD    L +   D+ YR WM+KYPSA+ SF++I+++AKGK IALFLDYDGTLSPIV
Sbjct: 78  TILNKD---NLSNDATDMTYREWMLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPIV 137

Query: 200 DNPDCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGS 259
           + PDCA+MS AMR+ V+  AKYFPTAIISGRSRDK          VYEF+ L ELYYAGS
Sbjct: 138 EEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDK----------VYEFVNLSELYYAGS 197

Query: 260 HGMDIMVSDRHSAN----------DQGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAGA 319
           HGMDIM     S N          +QGK+V +FQPASEFLP+ID+V  SLIE T+ I G 
Sbjct: 198 HGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIESTKDIKGV 257

Query: 320 KVENNKFCVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKA 379
           KVE+NKFC+SVHYRNV++KNW  +A CV D++  YP+LR++HGRKVLE+RPVI WDKGKA
Sbjct: 258 KVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPVIDWDKGKA 317

Query: 380 VTFLLESLGLSNCDEVLPIYVGDDCTDEDAFRVLKE-RNCGFGILVSSVTKDSSAAYSLR 439
           VTFLLESLGL+NC++VLPIYVGDD TDEDAF+VL++  N G+GILVS+V KDS+A YSLR
Sbjct: 318 VTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKDSNAFYSLR 377

Query: 440 DPSEVMEFLKSLVTWKKS 443
           DPSEVMEFLKSLVTWK+S
Sbjct: 378 DPSEVMEFLKSLVTWKRS 382

BLAST of HG10021778 vs. TAIR 10
Match: AT5G51460.3 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-134
Identity = 242/379 (63.85%), Postives = 294/379 (77.57%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPN---LLLIPRKKTGLLDDVRASSWLDAMKSSSPPP 139
           +  SRL +  N LPY++A     S N   LL +PRKKTG+LDDV+++ WLDAMKSSSPPP
Sbjct: 18  ISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDDVKSNGWLDAMKSSSPPP 77

Query: 140 NRISKDVVNELPSPDPDLLYRSWM-IKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPI 199
             ++KD    L +   D+ YR WM +KYPSA+ SF++I+++AKGK IALFLDYDGTLSPI
Sbjct: 78  TILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPI 137

Query: 200 VDNPDCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAG 259
           V+ PDCA+MS AMR+ V+  AKYFPTAIISGRSRDK          VYEF+ L ELYYAG
Sbjct: 138 VEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDK----------VYEFVNLSELYYAG 197

Query: 260 SHGMDIMVSDRHSAN----------DQGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAG 319
           SHGMDIM     S N          +QGK+V +FQPASEFLP+ID+V  SLIE T+ I G
Sbjct: 198 SHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIESTKDIKG 257

Query: 320 AKVENNKFCVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGK 379
            KVE+NKFC+SVHYRNV++KNW  +A CV D++  YP+LR++HGRKVLE+RPVI WDKGK
Sbjct: 258 VKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPVIDWDKGK 317

Query: 380 AVTFLLESLGLSNCDEVLPIYVGDDCTDEDAFRVLKE-RNCGFGILVSSVTKDSSAAYSL 439
           AVTFLLESLGL+NC++VLPIYVGDD TDEDAF+VL++  N G+GILVS+V KDS+A YSL
Sbjct: 318 AVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKDSNAFYSL 377

Query: 440 RDPSEVMEFLKSLVTWKKS 443
           RDPSEVMEFLKSLVTWK+S
Sbjct: 378 RDPSEVMEFLKSLVTWKRS 383

BLAST of HG10021778 vs. TAIR 10
Match: AT5G51460.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-134
Identity = 242/379 (63.85%), Postives = 294/379 (77.57%), Query Frame = 0

Query: 80  VIKSRLGVHSNMLPYAAAPGVGFSPN---LLLIPRKKTGLLDDVRASSWLDAMKSSSPPP 139
           +  SRL +  N LPY++A     S N   LL +PRKKTG+LDDV+++ WLDAMKSSSPPP
Sbjct: 18  ISNSRLTIRQNRLPYSSAAATAISQNNNLLLTVPRKKTGILDDVKSNGWLDAMKSSSPPP 77

Query: 140 NRISKDVVNELPSPDPDLLYRSWM-IKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPI 199
             ++KD    L +   D+ YR WM +KYPSA+ SF++I+++AKGK IALFLDYDGTLSPI
Sbjct: 78  TILNKD---NLSNDATDMTYREWMQLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPI 137

Query: 200 VDNPDCAFMSDAMRATVKEAAKYFPTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAG 259
           V+ PDCA+MS AMR+ V+  AKYFPTAIISGRSRDK          VYEF+ L ELYYAG
Sbjct: 138 VEEPDCAYMSSAMRSAVQNVAKYFPTAIISGRSRDK----------VYEFVNLSELYYAG 197

Query: 260 SHGMDIMVSDRHSAN----------DQGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAG 319
           SHGMDIM     S N          +QGK+V +FQPASEFLP+ID+V  SLIE T+ I G
Sbjct: 198 SHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQPASEFLPMIDKVLCSLIESTKDIKG 257

Query: 320 AKVENNKFCVSVHYRNVDDKNWNALANCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGK 379
            KVE+NKFC+SVHYRNV++KNW  +A CV D++  YP+LR++HGRKVLE+RPVI WDKGK
Sbjct: 258 VKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTYPKLRLTHGRKVLEIRPVIDWDKGK 317

Query: 380 AVTFLLESLGLSNCDEVLPIYVGDDCTDEDAFRVLKE-RNCGFGILVSSVTKDSSAAYSL 439
           AVTFLLESLGL+NC++VLPIYVGDD TDEDAF+VL++  N G+GILVS+V KDS+A YSL
Sbjct: 318 AVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLRDGPNHGYGILVSAVPKDSNAFYSL 377

Query: 440 RDPSEVMEFLKSLVTWKKS 443
           RDPSEVMEFLKSLVTWK+S
Sbjct: 378 RDPSEVMEFLKSLVTWKRS 383

BLAST of HG10021778 vs. TAIR 10
Match: AT4G22590.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 429.1 bits (1102), Expect = 4.3e-120
Identity = 224/351 (63.82%), Postives = 266/351 (75.78%), Query Frame = 0

Query: 103 SPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISKDVVNELPSPDPDLLYRSWMIK 162
           S   ++  RKK   LDDVR++ WLDAM SSSPP  R+ KD   E+ +P+ D   R+WM+K
Sbjct: 30  SGRFMMNSRKKIPKLDDVRSNGWLDAMISSSPPRKRLVKDFNIEI-APEDDFSQRAWMLK 89

Query: 163 YPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDCAFMSDAMRATVKEAAKYFPTA 222
           YPSA+ SF  I   AK K IA+FLDYDGTLSPIVD+PD A MSDAMRA VK+ AKYFPTA
Sbjct: 90  YPSAITSFAHIAAQAKNKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRAAVKDVAKYFPTA 149

Query: 223 IISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDIM--VSDRHSAND--------- 282
           IISGRSRDK          VY+ +GL ELYYAGSHGMDIM  V+   S  D         
Sbjct: 150 IISGRSRDK----------VYQLVGLTELYYAGSHGMDIMTPVNPNGSPEDPNCIKTTDQ 209

Query: 283 QGKEV-MFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNVDDKNWNALA 342
           QG+EV +FQPA EF+P+I+EVY +L+EIT+ I GAKVEN+KFC SVHYRNVD+K+W  +A
Sbjct: 210 QGEEVNLFQPAKEFIPVIEEVYNNLVEITKCIKGAKVENHKFCTSVHYRNVDEKDWPLVA 269

Query: 343 NCVYDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEVLPIYVGDDC 402
             V+D L+ YPRLR++HGRKVLEVRPVI W+KGKAV FLLESLGLSN DE LPI++GDD 
Sbjct: 270 QRVHDHLKRYPRLRITHGRKVLEVRPVIEWNKGKAVEFLLESLGLSNNDEFLPIFIGDDK 329

Query: 403 TDEDAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 442
           TDEDAF+VL+E N GFGILVSSV K+S+A YSLRDPSEV +FLK+LV W K
Sbjct: 330 TDEDAFKVLREGNRGFGILVSSVPKESNAFYSLRDPSEVKKFLKTLVKWGK 369

BLAST of HG10021778 vs. TAIR 10
Match: AT4G12430.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 427.9 bits (1099), Expect = 9.7e-120
Identity = 216/348 (62.07%), Postives = 266/348 (76.44%), Query Frame = 0

Query: 100 VGFSPNLLLIPRKKTGLLDDVRASSWLDAMKSSSPPPNRISKDVVNELPSPDPDLLYRSW 159
           +G S   ++   KK   LDDVR++ WLDAM SSSPP  ++ KD   E+ +P+ D   R+W
Sbjct: 24  LGVSSRFMMSQWKKPAKLDDVRSNGWLDAMISSSPPRKKLVKDFNVEV-APEDDFAQRAW 83

Query: 160 MIKYPSAVASFDQIITYAKGKSIALFLDYDGTLSPIVDNPDCAFMSDAMRATVKEAAKYF 219
           M+KYPSA++SF  I   AK K IA+FLDYDGTLSPIVD+PD A MSDAMR+ VK+ A YF
Sbjct: 84  MVKYPSAISSFAHIAAQAKKKKIAVFLDYDGTLSPIVDDPDRAIMSDAMRSAVKDVASYF 143

Query: 220 PTAIISGRSRDKFRAPLFLSVEVYEFIGLRELYYAGSHGMDIMVSDR-----HSANDQGK 279
           PTAIISGRSRDK          VY+ +GL ELYYAGSHGMDIM S        S + QGK
Sbjct: 144 PTAIISGRSRDK----------VYQLVGLTELYYAGSHGMDIMTSSDGPNCFKSTDQQGK 203

Query: 280 EV-MFQPASEFLPLIDEVYKSLIEITQGIAGAKVENNKFCVSVHYRNVDDKNWNALANCV 339
           EV +FQPA EF+P+IDEV+++L+E  + I GAKVEN+KFC SVHYRNVD+K+W  +A  V
Sbjct: 204 EVNLFQPAREFIPVIDEVFRTLVEKMKDIKGAKVENHKFCASVHYRNVDEKDWPIIAQRV 263

Query: 340 YDLLEDYPRLRVSHGRKVLEVRPVISWDKGKAVTFLLESLGLSNCDEVLPIYVGDDCTDE 399
           +D L+ YPRLR++HGRKVLEVRPVI W+KG+AV FLLESLGLSN D++LPIY+GDD TDE
Sbjct: 264 HDHLKQYPRLRLTHGRKVLEVRPVIDWNKGRAVEFLLESLGLSNKDDLLPIYIGDDTTDE 323

Query: 400 DAFRVLKERNCGFGILVSSVTKDSSAAYSLRDPSEVMEFLKSLVTWKK 442
           DAF+VL++ N GFGILVSS+ K+S+A YSLRDPSEV +FLK+LV W K
Sbjct: 324 DAFKVLRDGNRGFGILVSSIPKESNAFYSLRDPSEVKKFLKTLVKWAK 360