HG10021762 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021762 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 885.6 bits (2287), Expect = 2.2e-253
Identity = 470/527 (89.18%), Postives = 493/527 (93.55%), Query Frame = 0

Query: 3   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62
           SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61

Query: 63  FSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVL IG+SLGL+GYG QYLF+TNQ +SPSY VIF LTVLAGNSICWIN
Sbjct: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121

Query: 123 TVCYMVAITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLP 182
           TVCYMVAITNF TNRQIAVGISTSYQGLSAK+YTNIVSSIFS  SSKT ETFLLLNSVLP
Sbjct: 122 TVCYMVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLP 181

Query: 183 LGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSGLV 242
           +GVCVLA+PLAR++KVVEEQGKLEVGFFVIFVITIATGIFA VTSVG ISRMLSPLSGLV
Sbjct: 182 MGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLV 241

Query: 243 GILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC-E 302
           GI+VFLVLPL+VAVVEKVKES+KEG E RVYHFTVEENKDVER RGEN+ KLER+GD  E
Sbjct: 242 GIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEE 301

Query: 303 AMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 362
           AMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG
Sbjct: 302 AMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFG 361

Query: 363 FFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAVCT 422
           FFGRLMPSILD+FL R++CMKSKPGWMVGLMG LCGGFFLL+ P DVSLCVSTAIIAVCT
Sbjct: 362 FFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCT 421

Query: 423 GAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAANDAT 482
           GAITSISVSTTSELFGANNFSINHN+LVANIPFGSFLFGYMAAFLYHKQ     A  D T
Sbjct: 422 GAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQ-----AGGDGT 481

Query: 483 GKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQTSR 529
           GKCIGVECYRTTF IWGCF  FGTLLALLLFART+TFYSTT S TS+
Sbjct: 482 GKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTRSHTSK 523

BLAST of HG10021762 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 853.2 bits (2203), Expect = 1.2e-243
Identity = 457/527 (86.72%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFA-TNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLN 180
           INTVCYMVAITNFA +NRQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPL 240
           SVLPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATG FAT+TSVG +SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVER-NRGENERKLERE 300
           SGLVGI+VFLVLPL+V V EKVKE V+EG EGRVYHFTVEEN DVER  RGENERK+ER 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GDCEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
            + EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAI 420
           SSFGFFGRL+PSILD+FL RR + MKSKPGWMVGLMGALCGGFFLL+ P+D SLC+STAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAA 480
           IA+CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 ANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTN 524
           A    GKCIGVECYRTTF+IWGCF  FGT LALLLFARTR+FYSTT+
Sbjct: 481 A----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTD 523

BLAST of HG10021762 vs. NCBI nr
Match: XP_011658866.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN65862.1 hypothetical protein Csa_023247 [Cucumis sativus])

HSP 1 Score: 830.5 bits (2144), Expect = 8.4e-237
Identity = 446/527 (84.63%), Postives = 480/527 (91.08%), Query Frame = 0

Query: 3   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63

Query: 63  FSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICWIN
Sbjct: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123

Query: 123 TVCYMVAITNF-ATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLNSV 182
           TVCYMVAITNF +++RQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLNSV
Sbjct: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183

Query: 183 LPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSG 242
           LPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATGIFAT+TSVG +SRMLS L G
Sbjct: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243

Query: 243 LVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC 302
           LVGI+VFLVLPL+V VVEKVKE V+EG EG+VYHFTVEE  D ER RGENERK+ER  D 
Sbjct: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAV 422
           GFFGRL+PSILD+FL RR + MKSKPGWMVGLMG LCGGFFLL+ P+D SLC+STAIIA+
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIAI 423

Query: 423 CTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAAND 482
           CTGAITSISVSTT++LFGA NFSINHNI+VANIPFGSF+FGYMAAFLY KQA        
Sbjct: 424 CTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV---- 483

Query: 483 ATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQT 527
             GKCIGVECYRTTF+IWG F  FGT LALLLFART++FYSTT   +
Sbjct: 484 DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSS 526

BLAST of HG10021762 vs. NCBI nr
Match: KAA0059993.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ97252.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-228
Identity = 439/527 (83.30%), Postives = 467/527 (88.61%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFA-TNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLN 180
           INTVCYMVAITNFA +NRQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPL 240
           SVLPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATG FAT+TSVG +SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVER-NRGENERKLERE 300
           SGLVGI+VFLVLPL+V V EKVKE V+EG EGRVYHFTVEEN DVER  RGENERK+ER 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GDCEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
            + EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAI 420
           SSFGFFGRL+PSILD+FL RR + MKSKPGWMVGLMGALCG                   
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG------------------- 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAA 480
                GAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 421 -----GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 ANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTN 524
           A    GKCIGVECYRTTF+IWGCF  FGT LALLLFARTR+FYSTT+
Sbjct: 481 A----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTD 499

BLAST of HG10021762 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 794.3 bits (2050), Expect = 6.6e-226
Identity = 426/530 (80.38%), Postives = 465/530 (87.74%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL+IGSSLGL+GYG QYLFITNQF SPSY +IFFLTVLAGNSICW
Sbjct: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSV 180
           INTVCY+VAI NF +NRQ+AVGISTSYQGLSAK+YT+IV  IFS +S KTAETFLL NSV
Sbjct: 121 INTVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 180

Query: 181 LPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSG 240
            PLGVCVL +P+AR+V++VEEQG LEVGFFVIFVITIATG+FA VTSVG ISRMLSPLSG
Sbjct: 181 FPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 240

Query: 241 LVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC 300
           LVGILVFL+LP++V VVE VK+SV+EG E RVYH T+EE+    R R ENE KL R+G  
Sbjct: 241 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 300

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSF
Sbjct: 301 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSF 360

Query: 361 GFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAVC 420
           GFFGRLMPS+LDHFL RR+ MKSKPGW+V LMGA+CGGFFLL  P D+ LCVSTA+IAVC
Sbjct: 361 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVC 420

Query: 421 TGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAANDA 480
           TGAITSISVSTTSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA   A     
Sbjct: 421 TGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA----- 480

Query: 481 TGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQTSRDS 531
            GKCIGVECYRTTF+IWGC C FGTLLALLLFARTR+FYSTT S  ++ S
Sbjct: 481 -GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGS 519

BLAST of HG10021762 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.2e-72
Identity = 190/533 (35.65%), Postives = 292/533 (54.78%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VL   +++G +GYG Q+L ITN    P Y ++F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVC-- 188
            I +F  NR +A+ ++ S+ G+SA +Y    S  F+  +  ++  +LLLNS++PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALY----SLAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 189 ----VLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSGLV 248
               VL  P        + +      F ++ V+ + T     ++S    S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 249 GILVFLVLPLMVAVVEKVKESVKEGTEGRVYH-------FTVEENKD----VERNRGENE 308
           G +V LV PL   ++   ++        R+ H         ++E K+    V    G   
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 342

Query: 309 RKLEREGDCEAM-EEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSV 368
               +EG+   + +E   R ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S 
Sbjct: 343 MGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS- 402

Query: 369 SSLVSLSSSFGFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPN--DV 428
           ++LV++ SSF FFGRL+ +  D     +R   ++ GW    +      FFLL   +    
Sbjct: 403 TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 429 SLCVSTAIIAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYH 488
           +L  +TA+I + +G I + +VS TS+LFG N+  +NHNIL+ NIP GS L+GY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 489 KQAA--VAAAANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFY 520
             A+  +    +D+   CIG +CY  TF+ WGC    G + +L L+ RT+  Y
Sbjct: 523 ANASPDITPIVSDSI-VCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of HG10021762 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.8e-244
Identity = 457/527 (86.72%), Postives = 489/527 (92.79%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFA-TNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLN 180
           INTVCYMVAITNFA +NRQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPL 240
           SVLPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATG FAT+TSVG +SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVER-NRGENERKLERE 300
           SGLVGI+VFLVLPL+V V EKVKE V+EG EGRVYHFTVEEN DVER  RGENERK+ER 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GDCEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
            + EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAI 420
           SSFGFFGRL+PSILD+FL RR + MKSKPGWMVGLMGALCGGFFLL+ P+D SLC+STAI
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAI 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAA 480
           IA+CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 421 IAICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 ANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTN 524
           A    GKCIGVECYRTTF+IWGCF  FGT LALLLFARTR+FYSTT+
Sbjct: 481 A----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTD 523

BLAST of HG10021762 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 4.0e-237
Identity = 446/527 (84.63%), Postives = 480/527 (91.08%), Query Frame = 0

Query: 3   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63

Query: 63  FSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWIN 122
           FSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICWIN
Sbjct: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123

Query: 123 TVCYMVAITNF-ATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLNSV 182
           TVCYMVAITNF +++RQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLNSV
Sbjct: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183

Query: 183 LPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSG 242
           LPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATGIFAT+TSVG +SRMLS L G
Sbjct: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243

Query: 243 LVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC 302
           LVGI+VFLVLPL+V VVEKVKE V+EG EG+VYHFTVEE  D ER RGENERK+ER  D 
Sbjct: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDG 303

Query: 303 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 362
           EAMEEIGA+EM+KRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF
Sbjct: 304 EAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 363

Query: 363 GFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAV 422
           GFFGRL+PSILD+FL RR + MKSKPGWMVGLMG LCGGFFLL+ P+D SLC+STAIIA+
Sbjct: 364 GFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIAI 423

Query: 423 CTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAAND 482
           CTGAITSISVSTT++LFGA NFSINHNI+VANIPFGSF+FGYMAAFLY KQA        
Sbjct: 424 CTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGV---- 483

Query: 483 ATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQT 527
             GKCIGVECYRTTF+IWG F  FGT LALLLFART++FYSTT   +
Sbjct: 484 DPGKCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTKKSS 526

BLAST of HG10021762 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 5.3e-229
Identity = 439/527 (83.30%), Postives = 467/527 (88.61%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF
Sbjct: 1   MPSSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           ACFSGLAANYLPLWLVL IGSSLGLIGYG QYLFITNQF SPSYW+IFFLTVLAGNSICW
Sbjct: 61  ACFSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFA-TNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQ-SSKTAETFLLLN 180
           INTVCYMVAITNFA +NRQ+AVGISTSYQGLSAKVYT+IV SIFSN+ SSKTAETFLLLN
Sbjct: 121 INTVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLN 180

Query: 181 SVLPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPL 240
           SVLPLGVCVL +PLAR VK+VEEQGKLEVGFFVIFVITIATG FAT+TSVG +SRMLS L
Sbjct: 181 SVLPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSAL 240

Query: 241 SGLVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVER-NRGENERKLERE 300
           SGLVGI+VFLVLPL+V V EKVKE V+EG EGRVYHFTVEEN DVER  RGENERK+ER 
Sbjct: 241 SGLVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT 300

Query: 301 GDCEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360
            + EAMEEIGA+EM+KRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS
Sbjct: 301 DEGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLS 360

Query: 361 SSFGFFGRLMPSILDHFLPRR-RCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAI 420
           SSFGFFGRL+PSILD+FL RR + MKSKPGWMVGLMGALCG                   
Sbjct: 361 SSFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG------------------- 420

Query: 421 IAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAA 480
                GAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQA    A
Sbjct: 421 -----GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVA 480

Query: 481 ANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTN 524
           A    GKCIGVECYRTTF+IWGCF  FGT LALLLFARTR+FYSTT+
Sbjct: 481 A----GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTD 499

BLAST of HG10021762 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 3.2e-226
Identity = 426/530 (80.38%), Postives = 465/530 (87.74%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 1   MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLF 60

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           AC SGLAANYLPLW VL+IGSSLGL+GYG QYLFITNQF SPSY +IFFLTVLAGNSICW
Sbjct: 61  ACVSGLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICW 120

Query: 121 INTVCYMVAITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSV 180
           INTVCY+VAI NF +NRQ+AVGISTSYQGLSAK+YT+IV  IFS +S KTAETFLL NSV
Sbjct: 121 INTVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 180

Query: 181 LPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSG 240
            PLGVCVL +P+AR+V++VEEQG LEVGFFVIFVITIATG+FA VTSVG ISRMLSPLSG
Sbjct: 181 FPLGVCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 240

Query: 241 LVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC 300
           LVGILVFL+LP++V VVE VK+SV+EG E RVYH T+EE+    R R ENE KL R+G  
Sbjct: 241 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 300

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSF
Sbjct: 301 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSF 360

Query: 361 GFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAVC 420
           GFFGRLMPS+LDHFL RR+ MKSKPGW+V LMGA+CGGFFLL  P D+ LCVSTA+IAVC
Sbjct: 361 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVC 420

Query: 421 TGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAANDA 480
           TGAITSISVSTTSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA   A     
Sbjct: 421 TGAITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGA----- 480

Query: 481 TGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQTSRDS 531
            GKCIGVECYRTTF+IWGC C FGTLLALLLFARTR+FYSTT S  ++ S
Sbjct: 481 -GKCIGVECYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKSPRAQGS 519

BLAST of HG10021762 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 6.3e-222
Identity = 419/531 (78.91%), Postives = 461/531 (86.82%), Query Frame = 0

Query: 1   MPSSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLF 60
           MPSS  VFQWLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKLF
Sbjct: 55  MPSS--VFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLF 114

Query: 61  ACFSGLAANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICW 120
           AC SGLAANYLPLW +L+IGS LGL+GYG QYLFITNQF SPSY +IFFL+VLAGNSICW
Sbjct: 115 ACVSGLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICW 174

Query: 121 INTVCYMVAITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSV 180
           INTVCY+VAI NF +NRQ+AVGISTSYQGLSAK+YT+IV  IFS +S KTAETFLL NSV
Sbjct: 175 INTVCYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSV 234

Query: 181 LPLGVCVLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSG 240
            PLGVCVL  P+AR+V++VEE+G LEVGFFVIFVITIATG+FA VTSVG ISRMLSPLSG
Sbjct: 235 FPLGVCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSG 294

Query: 241 LVGILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREGDC 300
           LVGILVFL+LP++V VVE VK+SV+EG E RVYH T+EE+    R R ENE KL R+G  
Sbjct: 295 LVGILVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDGGD 354

Query: 301 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 360
           EAMEEIGA EMLKR+NFWLY GVY FGATLGLAFLNNLGQIAES GS SVSSLVSLSSSF
Sbjct: 355 EAMEEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSF 414

Query: 361 GFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIY-PNDVSLCVSTAIIAV 420
           GFFGRLMPS+LDHFL RR+ MKSKPGW+V LMGA+CGGFF L+  P D+ LCVSTA+IAV
Sbjct: 415 GFFGRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAV 474

Query: 421 CTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAAND 480
           CTGAITSISVSTTSELFGANNFSINHN++VANIPFGSFLFGYMAA+LY KQ      A  
Sbjct: 475 CTGAITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQ------ATG 534

Query: 481 ATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYSTTNSQTSRDS 531
            TGKCIGVECYRTTF+IWGC C  GTLLALLLFARTR+FYSTT S  ++ S
Sbjct: 535 DTGKCIGVECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKSPMAQGS 574

BLAST of HG10021762 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.1e-116
Identity = 244/520 (46.92%), Postives = 335/520 (64.42%), Query Frame = 0

Query: 7   VFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 66
           +  WLSLV I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+    SG+
Sbjct: 5   ILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGI 64

Query: 67  AANYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCY 126
           AA YLPL LVL+ G SLG  GYG QYL I  +                   ICWINT CY
Sbjct: 65  AAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINTACY 124

Query: 127 MVAITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVC 186
           +VAI +F  NRQ+AVGI+ SYQGLS K+YT++V S F     + A  +LLLNS++PL  C
Sbjct: 125 IVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVAC 184

Query: 187 VLAAPLAR---VVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSGLVG 246
           ++ AP+       K +     ++VGF V+FV+TIATGI+A  TS+  +  +L     LVG
Sbjct: 185 LVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV----LVG 244

Query: 247 ILVFLVLPLMVAVVEKVKESV-KEGTEGRVYHFTVEENK--DVERNRGENERKLEREGDC 306
           I +FL+ PL + +    KE +    T+ +V+      +K   VE +  + E + E+    
Sbjct: 245 IALFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKEEEEFEK-AII 304

Query: 307 EAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSF 366
              EE+   ++ K+++FW+YFG+YLFG T+GL F NNLGQIAESRGS++ SSLV+LSSSF
Sbjct: 305 GVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLVALSSSF 364

Query: 367 GFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPNDVSLCVSTAIIAVC 426
           GFFGRL+PS+LD+F  R + M S P  M G + A+   F LL+  +D++L + TA+I + 
Sbjct: 365 GFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMIGIF 424

Query: 427 TGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAANDA 486
           +GA+TS+SV+ T+ELFG  +F +NHNI+V +IP GSF FG +AA +Y   AA+       
Sbjct: 425 SGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDGAALYG----D 484

Query: 487 TGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFYS 521
            GKC G+ C++TT + WG  C    LLA +L+ R R FYS
Sbjct: 485 DGKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of HG10021762 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 3.7e-73
Identity = 190/533 (35.65%), Postives = 292/533 (54.78%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 69  NYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCYMV 128
            Y PL +VL   +++G +GYG Q+L ITN    P Y ++F   +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 129 AITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVC-- 188
            I +F  NR +A+ ++ S+ G+SA +Y    S  F+  +  ++  +LLLNS++PL V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALY----SLAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 189 ----VLAAPLARVVKVVEEQGKLEVGFFVIFVITIATGIFATVTSVGWISRMLSPLSGLV 248
               VL  P        + +      F ++ V+ + T     ++S    S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 249 GILVFLVLPLMVAVVEKVKESVKEGTEGRVYH-------FTVEENKD----VERNRGENE 308
           G +V LV PL   ++   ++        R+ H         ++E K+    V    G   
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 342

Query: 309 RKLEREGDCEAM-EEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSV 368
               +EG+   + +E   R ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S 
Sbjct: 343 MGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS- 402

Query: 369 SSLVSLSSSFGFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIYPN--DV 428
           ++LV++ SSF FFGRL+ +  D     +R   ++ GW    +      FFLL   +    
Sbjct: 403 TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 429 SLCVSTAIIAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFLYH 488
           +L  +TA+I + +G I + +VS TS+LFG N+  +NHNIL+ NIP GS L+GY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 489 KQAA--VAAAANDATGKCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFY 520
             A+  +    +D+   CIG +CY  TF+ WGC    G + +L L+ RT+  Y
Sbjct: 523 ANASPDITPIVSDSI-VCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of HG10021762 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 1.0e-70
Identity = 202/575 (35.13%), Postives = 306/575 (53.22%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VL   + +G +GYG Q+L ITN    P Y ++F   +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVCVL 188
            I NF  NR +A+ ++ S+ G+SA +YT   ++I    +  + E +LLLN+++PL V   
Sbjct: 127 CIRNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPVSTELYLLLNALVPLFVSFA 186

Query: 189 A-APLAR--VVKVVEEQG--KLEVGFFVIFVITIATGI----FATVTSVGWISRMLSPLS 248
           A  P+ R   ++ +   G  +  + F ++ ++ +  G+    F + TS    +R+L    
Sbjct: 187 ALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLL---- 246

Query: 249 GLVGILVFLVLPLMVA--------VVEKVKESVK-EGT-------------EGRVYHFTV 308
              G ++ L+LPL +          +  +  S + EG+             +G V     
Sbjct: 247 -FGGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREAS 306

Query: 309 EE-----NKDVER--NRGENERKLEREGDCEAMEEIGARE------------MLKRINFW 368
            E     N DV R  N  + +  +E +  C   + I   +            +L R +FW
Sbjct: 307 LEGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFW 366

Query: 369 LYFGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDHFLPR 428
           LY+  Y  G T+GL + NNLGQIA+S G SS  ++LV+L SSF FFGRL+ +  D+   R
Sbjct: 367 LYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI--R 426

Query: 429 RRCMKSKPGWM-VGLMGALCGGFFLLIYPNDVSLCVSTAIIAVCTGAITSISVSTTSELF 488
            +   ++ GW+ V L+      F L    +  +L   TA+I + +G I + +VS TSELF
Sbjct: 427 AKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELF 486

Query: 489 GANNFSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAAAANDATGKCIGVECYRTTFII 532
           G N+  +NHNIL+ NIP GS ++G++AA +Y   +   +        C+G +CY  TF+ 
Sbjct: 487 GPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVI--CMGRDCYLQTFMW 546

BLAST of HG10021762 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 1.1e-69
Identity = 194/560 (34.64%), Postives = 302/560 (53.93%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 69  NYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCYMV 128
            Y PLW VL   + +G +GYG Q+L IT+ F S  Y ++F   +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITH-FISLPYIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 129 AITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVCVL 188
            I+NF  NR +A+ ++ S+ G+SA +YT   ++I    +  + E +LLLN+++PL V   
Sbjct: 127 CISNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPTSPELYLLLNALIPLIVSFT 186

Query: 189 A-APLAR--VVKVVEEQG--KLEVGFFVIFVITIATGI----FATVTSVGWISRMLSPLS 248
           A  P+ R    + +   G  +  + F ++ ++    G+    F + +S    +R+L    
Sbjct: 187 AIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLL--FG 246

Query: 249 GLVGILVF-LVLPLMVAVVEKVKESVKEGTEGRVYHFTVEENKDVERNRGENERKLEREG 308
           G + +LVF L +P +V        ++          F + +  ++E ++G    +  REG
Sbjct: 247 GAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREG 306

Query: 309 -------------------------DC-------EAMEEIGARE----MLKRINFWLYFG 368
                                     C       + +E +G       +L R +FWLY+ 
Sbjct: 307 YQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYI 366

Query: 369 VYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDHFLPRRRCM 428
            Y  G T+GL + NNLGQIA+S G SS+ ++LV+L S+F FFGRL+ +  D+   R +  
Sbjct: 367 TYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI--RAKVY 426

Query: 429 KSKPGWM-VGLMGALCGGFFLLIYPNDVSLCVSTAIIAVCTGAITSISVSTTSELFGANN 488
            ++ GW+ + L+      F L       +L   TA++ + +G I + +VS TSELFG N+
Sbjct: 427 FARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNS 486

Query: 489 FSINHNILVANIPFGSFLFGYMAAFLYHKQAAVAA-AANDATGKCIGVECYRTTFIIWGC 520
             +NHNIL+ NIP GS ++G++AA +Y         +    +  C+G +CY  TF+ WGC
Sbjct: 487 VGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFVWWGC 546

BLAST of HG10021762 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 9.3e-69
Identity = 192/534 (35.96%), Postives = 290/534 (54.31%), Query Frame = 0

Query: 9   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68
           +W  LV  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 69  NYLPLWLVLMIGSSLGLIGYGFQYLFITNQFKSPSYWVIFFLTVLAGNSICWINTVCYMV 128
            Y P+  VL   +++GL+GYG Q+L I +    P Y ++     LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 129 AITNFATNRQIAVGISTSYQGLSAKVYTNIVSSIFSNQSSKTAETFLLLNSVLPLGVCVL 188
            I +F  N  +A+ +  S+ G+SA +YT +     S +SS +++ +LLLNS++PL V VL
Sbjct: 143 CIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 189 AA-PLARVVKVVEEQGKL---EVGFFVIF-VITIATGIFATVTSVGWISRMLSPLSGLVG 248
           A  P+       E        E   FV+F V+ + T  +  + S G      SP    +G
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHFLG 262

Query: 249 ILVFLVLPLMVAVVEKVKESVKEGTEGRVYHFT-------VEENKDV----ERNRGENER 308
            +  L+ PL V  ++ +  ++    E   +H +       +EE K +    +++  E E 
Sbjct: 263 AIFLLLFPLCVPFLDYIHRAL----ESCFHHHSSGYAAVNIEEPKILKIKSQKSNAEEES 322

Query: 309 KLEREGDCEAMEEIGAREMLKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRG--SSSV 368
              R GD     E     +++++ FWLY+  Y  G T+GL + NNLGQIA+S G  SS+ 
Sbjct: 323 DQVRLGD-----EHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNA 382

Query: 369 SSLVSLSSSFGFFGRLMPSILDHFLPRRRCMKSKPGWMVGLMGALCGGFFLLIY---PND 428
            SLV+L S+F F GRL+ S  D F  ++    ++ GW    +      FF+L Y    N 
Sbjct: 383 KSLVTLFSAFSFLGRLLSSAPD-FTRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQ 442

Query: 429 VSLC-VSTAIIAVCTGAITSISVSTTSELFGANNFSINHNILVANIPFGSFLFGYMAAFL 488
            +L  V+TA+I + +G + + +VS TSELFG N+  +N NIL+ NIP GS  +GYMA  +
Sbjct: 443 TALLEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSV 502

Query: 489 YHKQAAVAAAANDATG-KCIGVECYRTTFIIWGCFCCFGTLLALLLFARTRTFY 520
           Y   A++   +  A    C+G +CY  TF+ WGC    G + +L LF RTR  Y
Sbjct: 503 YDTNASLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVY 543