HG10021486 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021486 vs. NCBI nr
Match: XP_038894574.1 (alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1718.4 bits (4449), Expect = 0.0e+00
Identity = 843/914 (92.23%), Postives = 871/914 (95.30%), Query Frame = 0

Query: 1   MASGSKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVDP 60
           M SGSK++RTPFSQSLQP LLF ALL FL  FPSFFP   A SL AVGYGYRIRSSHVDP
Sbjct: 1   MGSGSKAMRTPFSQSLQPPLLFPALLFFL--FPSFFPNLVAGSLSAVGYGYRIRSSHVDP 60

Query: 61  AGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRS 120
           AGKSLTA LDLIRTSPV GPD+ RLTLQATFET+DRLRIRITDSTRERWEIPDQIIPRRS
Sbjct: 61  AGKSLTADLDLIRTSPVNGPDVPRLTLQATFETKDRLRIRITDSTRERWEIPDQIIPRRS 120

Query: 121 NSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDP 180
           +SRIRSLPENHVGSP+ SFISDPASDLIFTLH TAPFGFSVLRRSSGDVLFDTSPD SD 
Sbjct: 121 SSRIRSLPENHVGSPRTSFISDPASDLIFTLHYTAPFGFSVLRRSSGDVLFDTSPDLSDS 180

Query: 181 ETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDVN 240
           ETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKL+PDKNKTLTLWNADIGS+NLDVN
Sbjct: 181 ETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLMPDKNKTLTLWNADIGSVNLDVN 240

Query: 241 LYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFA 300
           LYGAHPFYIDVRSPS DGKVAAGTTHGVLLLNSNGMDIIYSG R+ YKVIGGIIDLYFFA
Sbjct: 241 LYGAHPFYIDVRSPSGDGKVAAGTTHGVLLLNSNGMDIIYSGHRIMYKVIGGIIDLYFFA 300

Query: 301 GPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWT 360
           GPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSD+ESVVARYAKA IPLEV+WT
Sbjct: 301 GPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDLESVVARYAKAGIPLEVMWT 360

Query: 361 DIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKA 420
           DIDYMD YKDFTFDP+NFP EKMKKFVDNLHKNGQKYVLILDPGISTNNTYGT+IRGK+A
Sbjct: 361 DIDYMDGYKDFTFDPVNFPAEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTFIRGKRA 420

Query: 421 DIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNF 480
           DIFI+Y+GVPYLGEVWPG VYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNF
Sbjct: 421 DIFIQYDGVPYLGEVWPGPVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNF 480

Query: 481 ITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATH 540
           ITSSTSPFSNLDNPPYMIN+A VRRPLNNKTVPAS+LHFGNLTEYNTHNLYGFLESKATH
Sbjct: 481 ITSSTSPFSNLDNPPYMINNAGVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESKATH 540

Query: 541 ASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGA 600
           ASL+KVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDL  +IPSILNFGLFGIPMVGA
Sbjct: 541 ASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLRNTIPSILNFGLFGIPMVGA 600

Query: 601 DICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRL 660
           DICGFSGDTTEELC RWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRL
Sbjct: 601 DICGFSGDTTEELCGRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRL 660

Query: 661 LPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVDA 720
           LPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTY INSQFLLG GVLVSPVLKEGAVSVDA
Sbjct: 661 LPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYGINSQFLLGEGVLVSPVLKEGAVSVDA 720

Query: 721 YFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARETA 780
           YFP GNWFSLFNYSELVAVKSGQ+ITLDAPADHINVHVREGNILALHG+AMTT+AARETA
Sbjct: 721 YFPTGNWFSLFNYSELVAVKSGQRITLDAPADHINVHVREGNILALHGKAMTTRAARETA 780

Query: 781 YKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVINGGFA 840
           Y+LLV VSN QSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSE VGSKL+VKSQVINGGFA
Sbjct: 781 YELLVVVSNDQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEAVGSKLVVKSQVINGGFA 840

Query: 841 LSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISGLSI 900
           LSQ LIIDKVTFVGFERPKKM  L LNISKGV L GNSTIRKTY+YF K MN+EISGLSI
Sbjct: 841 LSQNLIIDKVTFVGFERPKKMVDLGLNISKGVKLNGNSTIRKTYKYFAKVMNVEISGLSI 900

Query: 901 PIWEEFILEMTPIS 915
           PIWEEF+LEMTPIS
Sbjct: 901 PIWEEFMLEMTPIS 912

BLAST of HG10021486 vs. NCBI nr
Match: XP_011660330.1 (alpha-glucosidase [Cucumis sativus] >KGN66921.1 hypothetical protein Csa_007658 [Cucumis sativus])

HSP 1 Score: 1661.4 bits (4301), Expect = 0.0e+00
Identity = 813/915 (88.85%), Postives = 857/915 (93.66%), Query Frame = 0

Query: 1   MASG-SKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVD 60
           MASG SK IRTPF  SL         +L L  F SF PLPAA SLPAVG GYRIRSSHVD
Sbjct: 15  MASGSSKVIRTPFPHSLP--------ILILFLFTSFLPLPAA-SLPAVGLGYRIRSSHVD 74

Query: 61  PAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRR 120
           PAGK+LTA LDLI TS VYGPDL  LTLQATFE++DRLR+RITDSTRERWE+P  I+PR 
Sbjct: 75  PAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRP 134

Query: 121 SNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSD 180
           S+S IRSLPENHV SPKASFIS PASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSP FSD
Sbjct: 135 SSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSD 194

Query: 181 PETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDV 240
            ETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS+NLDV
Sbjct: 195 SETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDV 254

Query: 241 NLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFF 300
           NLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDI+YSGDR+TYKVIGGIIDLYFF
Sbjct: 255 NLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFF 314

Query: 301 AGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLW 360
           AGPSPISV+DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLE +W
Sbjct: 315 AGPSPISVVDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMW 374

Query: 361 TDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKK 420
           TDIDYMD YKDFTFDPINFP +KMK FVDNLHKNGQKYVLILDPGISTNNTYG YIRG K
Sbjct: 375 TDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTK 434

Query: 421 ADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISN 480
           ADIF+KYNGVPYLG+VWPG VYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEISN
Sbjct: 435 ADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISN 494

Query: 481 FITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKAT 540
           FITSSTSP SNLDNPPYMIN+ARV+RPLNNKTVPAS+LHFGNLTEYNTHNLYGFLES+AT
Sbjct: 495 FITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRAT 554

Query: 541 HASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVG 600
           HASL+KVTG+RPFVLSRSTFVGSGKYTAHWTGDNGATWNDL Y+IPSILNFGLFGIPMVG
Sbjct: 555 HASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVG 614

Query: 601 ADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYR 660
           +DICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY+
Sbjct: 615 SDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQ 674

Query: 661 LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVD 720
           LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT+EI+SQFLLGGGVLVSPVLKEGA SVD
Sbjct: 675 LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVD 734

Query: 721 AYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARET 780
           AYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGEAMTT+AA+ET
Sbjct: 735 AYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQET 794

Query: 781 AYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVINGGF 840
            YKLLV +SNGQSSFGEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKL+VKSQVINGGF
Sbjct: 795 PYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGF 854

Query: 841 ALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISGLS 900
           ALSQKLIIDKVTFVGF+RPKKM  L LNISKG+NL GNS+IRKTYQYF KFMN+EISGLS
Sbjct: 855 ALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLS 914

Query: 901 IPIWEEFILEMTPIS 915
           IPIWEEFILEMTPI+
Sbjct: 915 IPIWEEFILEMTPIN 920

BLAST of HG10021486 vs. NCBI nr
Match: XP_008459069.1 (PREDICTED: alpha-glucosidase [Cucumis melo] >KAA0043261.1 alpha-glucosidase [Cucumis melo var. makuwa] >TYK29325.1 alpha-glucosidase [Cucumis melo var. makuwa])

HSP 1 Score: 1658.7 bits (4294), Expect = 0.0e+00
Identity = 813/917 (88.66%), Postives = 854/917 (93.13%), Query Frame = 0

Query: 1   MASGSK---SIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSH 60
           MASGS    +IRTPF+ SL        L+L L  F SF   P A SLPAVG+GYRIRSSH
Sbjct: 1   MASGSSKAITIRTPFAHSL--------LILILFLFTSFLIPPPAASLPAVGFGYRIRSSH 60

Query: 61  VDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIP 120
           +DPAGKSLTA L LIRTS VYGPDL  LTLQATFE++DRLR+RITDSTRERWEIPD IIP
Sbjct: 61  IDPAGKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIP 120

Query: 121 RRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDF 180
           R SNS IRSLPENHV SPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDF
Sbjct: 121 RPSNSLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDF 180

Query: 181 SDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNL 240
           S+ ETF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS+NL
Sbjct: 181 SNSETFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNL 240

Query: 241 DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLY 300
           DVNLYGAHPFYIDVRS SRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLY
Sbjct: 241 DVNLYGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLY 300

Query: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEV 360
           FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLEV
Sbjct: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEV 360

Query: 361 LWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRG 420
           +WTDIDYMD YKDFTFDPINFP EKMK FVDNLHKNGQKYV+ILDPGISTNNTYG YIRG
Sbjct: 361 MWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRG 420

Query: 421 KKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEI 480
            KADIF+K++GVPYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNEI
Sbjct: 421 TKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEI 480

Query: 481 SNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESK 540
           SNFITSSTSP SNLDNPPYMIN+ARVRRPLNNKTVPAS+LHFGNLTEYNTHNLYGFLES+
Sbjct: 481 SNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESR 540

Query: 541 ATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPM 600
           ATHASL+KVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+ Y+IPSILNFGLFGIPM
Sbjct: 541 ATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPM 600

Query: 601 VGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALR 660
           VG+DICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALR
Sbjct: 601 VGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALR 660

Query: 661 YRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVS 720
           Y+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPVLKEGA S
Sbjct: 661 YQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFS 720

Query: 721 VDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAAR 780
           VDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGEAMTTQAAR
Sbjct: 721 VDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAAR 780

Query: 781 ETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVING 840
           ETAYKLLV +SNGQ SFGEVFLDDGEV EMG EGGNWS+VRF SE VGSKL+VKSQVING
Sbjct: 781 ETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVING 840

Query: 841 GFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISG 900
           GFALSQKLIIDKVTFVGFERPKKM GL LNISKGV+L GNS+IRKTYQYF KFMN+EISG
Sbjct: 841 GFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISG 900

Query: 901 LSIPIWEEFILEMTPIS 915
           LSIPIWEEFILEMTPIS
Sbjct: 901 LSIPIWEEFILEMTPIS 909

BLAST of HG10021486 vs. NCBI nr
Match: XP_023001112.1 (alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1613.6 bits (4177), Expect = 0.0e+00
Identity = 794/924 (85.93%), Postives = 851/924 (92.10%), Query Frame = 0

Query: 1   MASGSKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLP----------AVGYG 60
           MA+GSKS++      L+   L SA++LFL  F  FF L A  SLP          AVGYG
Sbjct: 22  MATGSKSLQA----FLRRRRLLSAVVLFL--FSCFFRLIA--SLPFAAGEQRENSAVGYG 81

Query: 61  YRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWE 120
           YR+RS  VDP GKSLTA LDLI  S VYGPD+ RL+LQA+FET+DRLR+RITDS RERWE
Sbjct: 82  YRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWE 141

Query: 121 IPDQIIPRRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVL 180
           IPD+IIPRRSNSRIRSLPE  VGSP+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVL
Sbjct: 142 IPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDVL 201

Query: 181 FDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNA 240
           FDTSP+FSD ETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFK+VPDK+KTLTLW+A
Sbjct: 202 FDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSA 261

Query: 241 DIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVI 300
           DIGS+N DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK I
Sbjct: 262 DIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAI 321

Query: 301 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAK 360
           GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYAK
Sbjct: 322 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAK 381

Query: 361 ASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNT 420
           A IPLEV+WTDIDYMD YKDFTFDPINFPVEKMK FVD+LH+NGQKYVLI+DPGISTN T
Sbjct: 382 AGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNKT 441

Query: 421 YGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGL 480
           YGTYIRG +ADIFIKY+GVPYLGEVWPG VYFPDFLHPNSE FWGGEI+LFRDIVPFDGL
Sbjct: 442 YGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDGL 501

Query: 481 WIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNL 540
           WIDMNE+SNFITSSTSPFSNLDNPPY I++A V+RP+NN+TVPAS LHFGNLTEYNTHNL
Sbjct: 502 WIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHNL 561

Query: 541 YGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNF 600
           YGFLESKATHASL+KVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDL Y+IPSILNF
Sbjct: 562 YGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNF 621

Query: 601 GLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 660
           GLFGIPMVGADICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA
Sbjct: 622 GLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 681

Query: 661 RKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPV 720
           RKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVLVSPV
Sbjct: 682 RKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSPV 741

Query: 721 LKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEA 780
           LKEGA+SVDAYFPAGNWFSLFNYSE VAVKSGQQITLDAPADHINVHVREGNILALHGEA
Sbjct: 742 LKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGEA 801

Query: 781 MTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLV 840
            TTQAAR+TA+KLLV VSNGQSS GEVFLDDGEVVEMG EGGNWSLVRFYSE VGSKLL+
Sbjct: 802 TTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLI 861

Query: 841 KSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKF 900
           KSQVINGGFALSQK+IIDKVTFVGFERPKKMGGL L+ISKG NL GNS IRKTY+Y  KF
Sbjct: 862 KSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAKF 921

Query: 901 MNIEISGLSIPIWEEFILEMTPIS 915
           +N+EISGLSIPI EEF++E++P++
Sbjct: 922 VNVEISGLSIPICEEFVMELSPLN 937

BLAST of HG10021486 vs. NCBI nr
Match: XP_023520110.1 (alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1609.0 bits (4165), Expect = 0.0e+00
Identity = 791/924 (85.61%), Postives = 845/924 (91.45%), Query Frame = 0

Query: 1   MASGSKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLP----------AVGYG 60
           MA+GSKS++      L+   L SA++LFL  F  FF L A  SLP          AVGYG
Sbjct: 22  MATGSKSLQA----FLRRRRLLSAVVLFL--FTCFFRLTA--SLPFAAGEQRENSAVGYG 81

Query: 61  YRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWE 120
           YR+RS  VDP GKSLTA LDLI  S VYGPD+ RL++QA+FET+DRLR+RITDS RERWE
Sbjct: 82  YRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSIQASFETKDRLRVRITDSNRERWE 141

Query: 121 IPDQIIPRRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVL 180
           +PD+IIPRRSNSRIRSLPE  VGSP+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVL
Sbjct: 142 VPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVL 201

Query: 181 FDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNA 240
           FDTSP+FSD ETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFKLVPDK+KTLTLWNA
Sbjct: 202 FDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNA 261

Query: 241 DIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVI 300
           DIGS+N DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK I
Sbjct: 262 DIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAI 321

Query: 301 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAK 360
           GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYAK
Sbjct: 322 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAK 381

Query: 361 ASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNT 420
           A IPLEV+WTDIDYMD YKDFTFDPINFPVEKMK FVDNLH+NGQKYVLILDPGISTN T
Sbjct: 382 AGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKT 441

Query: 421 YGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGL 480
           YGTYIRG +ADIFIKY+GVPYLGEVWPG VYFPDFLHPNSE FWG EI+LFRDIVPFDGL
Sbjct: 442 YGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLFRDIVPFDGL 501

Query: 481 WIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNL 540
           WIDMNEISNFITSSTS FSNLDNPPY IN+A V+RP+NN+TVPAS LHFGNLTEYNTHNL
Sbjct: 502 WIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNL 561

Query: 541 YGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNF 600
           YGFLESKATHASL+KVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDL Y+IPSILNF
Sbjct: 562 YGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNF 621

Query: 601 GLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 660
           GLFGIPMVGADICGFS DTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA
Sbjct: 622 GLFGIPMVGADICGFSRDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 681

Query: 661 RKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPV 720
           RKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVL+SPV
Sbjct: 682 RKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPV 741

Query: 721 LKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEA 780
           LKEGA+SVDAYFPAGNWFSLFNYSE VAV SGQQITLDAPADHINVHVREGNILALHGEA
Sbjct: 742 LKEGAISVDAYFPAGNWFSLFNYSESVAVNSGQQITLDAPADHINVHVREGNILALHGEA 801

Query: 781 MTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLV 840
            TTQAAR+TA+KLLV VSNGQSS GEVFLDDGEVVEMG EGGNWSLVRFYSE VGSKLL+
Sbjct: 802 TTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLI 861

Query: 841 KSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKF 900
           KSQVINGGFALSQK+IIDKVT VGFERPK MGGL L+ISKG NL GNS IRKTY+Y  KF
Sbjct: 862 KSQVINGGFALSQKMIIDKVTIVGFERPKNMGGLGLDISKGANLDGNSGIRKTYEYSAKF 921

Query: 901 MNIEISGLSIPIWEEFILEMTPIS 915
           +N+EISGLSI IWEEF++E++P++
Sbjct: 922 VNVEISGLSISIWEEFVMELSPLN 937

BLAST of HG10021486 vs. ExPASy Swiss-Prot
Match: O04893 (Alpha-glucosidase OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 1098.6 bits (2840), Expect = 0.0e+00
Identity = 537/902 (59.53%), Postives = 675/902 (74.83%), Query Frame = 0

Query: 18  PSLLFSALLLFLL-YFPSFFPLPAANSLPAVGYGYRIRSSHVDP-AGKSLTAHLDLIRTS 77
           PSL    LL+FLL Y  +       +    +GYGY+++S  VD    +SLTA   L++ S
Sbjct: 6   PSLALGILLVFLLQYLVAGISTSENDPEGVIGYGYKVKSVKVDSGTRRSLTALPQLVKNS 65

Query: 78  PVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQII---------PRRSNSRIRS 137
            VYGPD+  L++ A+ E+ DRLR+RITD+   RWEIPD I+         P   +S  R+
Sbjct: 66  SVYGPDIQLLSITASLESNDRLRVRITDAKHRRWEIPDNILHRHQPPPPPPHSLSSLYRT 125

Query: 138 LPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVF 197
           L  +   + +   +S P SDL F+L +T PFGF++ R+S+ DVLFD +PD ++P TFL+F
Sbjct: 126 LLSSPTTNRRKILLSHPNSDLTFSLINTTPFGFTISRKSTHDVLFDATPDPTNPNTFLIF 185

Query: 198 KDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHP 257
            DQY+ L+SSLP  R+ I+G+GE ++ +F+L    N+TLT+  ADI S N DVNLYG+HP
Sbjct: 186 IDQYLHLTSSLPGTRAHIYGLGEHSKPTFQLA--HNQTLTMRAADIPSSNPDVNLYGSHP 245

Query: 258 FYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPIS 317
           FY+DVRS        AG+THGVLLLNSNGMD+ Y+G+R+TYKVIGGIIDLYFFAGPSP  
Sbjct: 246 FYMDVRS-----SPVAGSTHGVLLLNSNGMDVEYTGNRITYKVIGGIIDLYFFAGPSPGQ 305

Query: 318 VIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMD 377
           V++Q+T +IGRPAP+PYW+FGF QCRYGY +V +++SVVA YAKA IPLEV+WTDIDYMD
Sbjct: 306 VVEQFTRVIGRPAPMPYWAFGFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMD 365

Query: 378 EYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFIKY 437
            YKDFT DP+NFP++KMKKFV+NLHKNGQKYV+ILDPGISTN TY TYIRG K D+F+K 
Sbjct: 366 AYKDFTLDPVNFPLDKMKKFVNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKR 425

Query: 438 NGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFITSSTS 497
           NG PYLG VWPG VYFPDFL P++  FW  EI+ F +++P DGLWIDMNEISNFI+S   
Sbjct: 426 NGKPYLGSVWPGPVYFPDFLKPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPI 485

Query: 498 PFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATHASLIKV 557
           P S LDNPPY IN++ V  P+ NKT+P + +H+G++ EYN HNL+G+LE++ T A+LIK+
Sbjct: 486 PGSTLDNPPYKINNSGVMLPIINKTIPPTAMHYGDIPEYNVHNLFGYLEARVTRAALIKL 545

Query: 558 TGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGADICGFS 617
           T KRPFVLSRSTF GSGKYTAHWTGDN ATWNDL YSIPS+L+FGLFGIPMVGADICGF 
Sbjct: 546 TEKRPFVLSRSTFSGSGKYTAHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFL 605

Query: 618 GDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLLPYFYT 677
           G+TTEELC+RWIQLGAFYPF+RDHS  G+  QELY W++VAASARKVL LRY LLPYFYT
Sbjct: 606 GNTTEELCRRWIQLGAFYPFSRDHSSLGTTYQELYRWESVAASARKVLGLRYTLLPYFYT 665

Query: 678 LMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVDAYFPAGN 737
           LMYEA   G PIARPLFFSFP DIKTY I+SQFLLG GV+VSPVLK G VSV AYFP GN
Sbjct: 666 LMYEAQLNGIPIARPLFFSFPDDIKTYGISSQFLLGKGVMVSPVLKPGVVSVTAYFPRGN 725

Query: 738 WFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVA 797
           WF LF+Y+  V   +G+ +TL AP DHINVH++EGNILA+ G+AMTTQAAR+T + LLV 
Sbjct: 726 WFDLFDYTRSVTASTGRYVTLSAPPDHINVHIQEGNILAMQGKAMTTQAARKTPFHLLVV 785

Query: 798 VSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVINGGFALSQKLI 857
           +S+  +SFGE+FLDDG  V MG   G W+ V+F +       ++ S V++G FA+SQK +
Sbjct: 786 MSDCGASFGELFLDDGVEVTMGVNRGKWTFVKFIAASAKQTCIITSDVVSGEFAVSQKWV 845

Query: 858 IDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISGLSIPIWEEF 909
           IDKVT +G  +  K+ G ++           S ++ T     +F+  EISGL++ +  EF
Sbjct: 846 IDKVTILGLRKGTKINGYTVRTGAVTRKGDKSKLKSTPDRKGEFIVAEISGLNLLLGREF 900

BLAST of HG10021486 vs. ExPASy Swiss-Prot
Match: O04931 (Alpha-glucosidase OS=Beta vulgaris OX=161934 PE=1 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 1.4e-311
Identity = 506/879 (57.57%), Postives = 675/879 (76.79%), Query Frame = 0

Query: 42  NSLPAVGYGYRIRSSHVD-PAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIR 101
           N   A+GYGY+++++ VD   GKSLTA L LIR SPVYGPD+  L+  A+FE +D LRIR
Sbjct: 36  NQGEAIGYGYQVKNAKVDNSTGKSLTALLQLIRNSPVYGPDIHFLSFTASFEEDDTLRIR 95

Query: 102 ITDSTRERWEIPDQIIPRRSN-------SRIRSLPEN-HVGSPKASFISDPASDLIFTLH 161
            TD+   RWEIP++++PR          S ++ LP+      P  + +S P SDL FTL 
Sbjct: 96  FTDANNRRWEIPNEVLPRPPPPPSPPPLSSLQHLPKPIPQNQPTTTVLSHPHSDLAFTLF 155

Query: 162 DTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR 221
            T PFGF++ R+S+ DVLFD +P  S+P TFL++KDQY+QLSSSLP  ++ ++G+GE T+
Sbjct: 156 HTTPFGFTIYRKSTHDVLFDATPIPSNPTTFLIYKDQYLQLSSSLPAQQAHLYGLGEHTK 215

Query: 222 ESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLN 281
            +F+L    N+ LTLWNADI S N D+NLYG+HPFY+DVRS         G+THGV LLN
Sbjct: 216 PTFQLA--HNQILTLWNADIASFNRDLNLYGSHPFYMDVRS-----SPMVGSTHGVFLLN 275

Query: 282 SNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCR 341
           SNGMD+ Y+GDR+TYKVIGGIIDLY FAG +P  V+DQYT+LIGRPAP+PYW+FGFHQCR
Sbjct: 276 SNGMDVEYTGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCR 335

Query: 342 YGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHK 401
           +GY++V++IE+VV +YA+A IPLEV+WTDIDYMD +KDFT DP++FP++KM++FV  LH+
Sbjct: 336 WGYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHR 395

Query: 402 NGQKYVLILDPGISTNNTYGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSEN 461
           NGQ+YV ILDPGI+TN +YGT+IRG ++++FIK NG PYLG VWPG VY+PDFL P + +
Sbjct: 396 NGQRYVPILDPGINTNKSYGTFIRGMQSNVFIKRNGNPYLGSVWPGPVYYPDFLDPAARS 455

Query: 462 FWGGEIQLFRDIVPFDGLWIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTV 521
           FW  EI+ FRDI+P DG+WIDMNE SNFITS+ +P S LDNPPY IN++  R P+N+KT+
Sbjct: 456 FWVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTI 515

Query: 522 PASVLHFGNLTEYNTHNLYGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGD 581
           PA+ +H+GN+TEYN HNLYGFLES+AT  +L++   + PF+LSRSTF GSGKYTAHWTGD
Sbjct: 516 PATAMHYGNVTEYNAHNLYGFLESQATREALVRPATRGPFLLSRSTFAGSGKYTAHWTGD 575

Query: 582 NGATWNDLEYSIPSILNFGLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSD 641
           N A W+DL+YSIP++LNFGLFG+PM+GADICGF+  TTEELC RWIQLGAFYPF+RDHS 
Sbjct: 576 NAARWDDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSA 635

Query: 642 KGSIRQELYLWDTVAASARKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT 701
           + +  QELYLW++VAASAR VL LRY LLPY+YTLMY+A+ +G+PIARPL F+FP D+ T
Sbjct: 636 RDTTHQELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVAT 695

Query: 702 YEINSQFLLGGGVLVSPVLKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPAD 761
           Y I+SQFL+G G++VSPVL+ G+  V+AY P GNW SL NY+  V+V +G  ++L AP D
Sbjct: 696 YGISSQFLIGRGIMVSPVLQPGSSIVNAYSPRGNWVSLSNYTSSVSVSAGTYVSLSAPPD 755

Query: 762 HINVHVREGNILALHGEAMTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGG 821
           HINVH+ EGNI+A+ GEAMTTQAAR T + LLV +S+  +S GE+FLD+G  +++G  GG
Sbjct: 756 HINVHIHEGNIVAMQGEAMTTQAARSTPFHLLVVMSDHVASTGELFLDNGIEMDIGGPGG 815

Query: 822 NWSLVRFYSEVVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKG- 881
            W+LVRF++E   + L + S+V+N G+A+SQ+ ++DK+T +G +R  K+   ++    G 
Sbjct: 816 KWTLVRFFAESGINNLTISSEVVNRGYAMSQRWVMDKITILGLKRRVKIKEYTVQKDAGA 875

Query: 882 VNLKGNSTIRKTYQYFTKFMNIEISGLSIPIWEEFILEM 911
           + +KG      ++     F+++ IS L   + + F LE+
Sbjct: 876 IKVKGLGRRTSSHNQGGFFVSV-ISDLRQLVGQAFKLEL 906

BLAST of HG10021486 vs. ExPASy Swiss-Prot
Match: Q653V7 (Probable alpha-glucosidase Os06g0675700 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0675700 PE=1 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 6.5e-293
Identity = 501/905 (55.36%), Postives = 653/905 (72.15%), Query Frame = 0

Query: 18  PSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVDPAGKSLTAHLDLI----R 77
           P+    AL +FLL    F   P       V  GY + S  V  +   L A L+L      
Sbjct: 9   PARRLGALAVFLLAL--FLAAPW-----GVDCGYNVAS--VAGSKNRLRARLELAGGGGG 68

Query: 78  TSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRSNSRIRSLPENHVG 137
            +P  GPD+ RL+L A+ ET+ RL +RITD+   RWE+P  +IPR S       P++ + 
Sbjct: 69  AAPELGPDVRRLSLTASLETDSRLHVRITDADHPRWEVPQDVIPRPS-------PDSFLA 128

Query: 138 SPK---ASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVFKDQY 197
           + +      +S   SDL F +H T+PF F+V RRS+GDVLFDT+P+       LVFKD+Y
Sbjct: 129 ATRPGGGRVLSTATSDLTFAIH-TSPFRFTVTRRSTGDVLFDTTPN-------LVFKDRY 188

Query: 198 IQLSSSL-PKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYI 257
           ++L+SSL P  R+S++G+GEQT+ +F+L   +N T TLWN+DI + N+D+NLYG+HPFY+
Sbjct: 189 LELTSSLPPPGRASLYGLGEQTKRTFRL--QRNDTFTLWNSDIAAGNVDLNLYGSHPFYM 248

Query: 258 DVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVID 317
           DVRS    G    G  HGVLLLNSNGMD+IY G  VTYKVIGG++D YFFAGPSP++V+D
Sbjct: 249 DVRSGGGGG---GGAAHGVLLLNSNGMDVIYGGSYVTYKVIGGVLDFYFFAGPSPLAVVD 308

Query: 318 QYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYK 377
           QYT+LIGRPAP+PYWSFGFHQCRYGYKNV+D+E VVA YAKA IPLEV+WTDIDYMD YK
Sbjct: 309 QYTQLIGRPAPMPYWSFGFHQCRYGYKNVADLEGVVAGYAKARIPLEVMWTDIDYMDAYK 368

Query: 378 DFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFIKYNGV 437
           DFT DP+NFP ++M+ FVD LH+NGQK+V+I+DPGI+ N TYGT++RG K DIF+K+NG 
Sbjct: 369 DFTLDPVNFPADRMRPFVDRLHRNGQKFVVIIDPGINVNTTYGTFVRGMKQDIFLKWNGS 428

Query: 438 PYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFITSSTSPFS 497
            YLG VWPG VYFPDFL+P +  FW  EI  FR  +P DGLW+DMNEISNF+     P +
Sbjct: 429 NYLGVVWPGNVYFPDFLNPRAAEFWAREIAAFRRTLPVDGLWVDMNEISNFV--DPPPLN 488

Query: 498 NLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATHASLIKVTGK 557
            +D+PPY IN++ VRRP+NNKTVPAS +H+G + EY+ HNL+GFLE++ATH +L++ TG+
Sbjct: 489 AIDDPPYRINNSGVRRPINNKTVPASAVHYGGVAEYDAHNLFGFLEARATHDALLRDTGR 548

Query: 558 RPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGADICGFSGDT 617
           RPFVLSRSTFVGSG+YTAHWTGDN ATW DL YSI ++L+FGLFGIPM+GADICGF G+T
Sbjct: 549 RPFVLSRSTFVGSGRYTAHWTGDNAATWEDLHYSINTMLSFGLFGIPMIGADICGFGGNT 608

Query: 618 TEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLLPYFYTLMY 677
           TEELC RWIQLGAFYPF+RDHS  G++R+ELYLW++VA SARK L LRYRLLPY YTLMY
Sbjct: 609 TEELCSRWIQLGAFYPFSRDHSAIGTVRRELYLWESVARSARKALGLRYRLLPYLYTLMY 668

Query: 678 EAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVDAYFPAGNWFS 737
           EAH  G PIARPLFFS+P D++TY I+ QFLLG GVLVSPVL+ GA +V AYFPAG WFS
Sbjct: 669 EAHTTGAPIARPLFFSYPGDVETYGIDRQFLLGRGVLVSPVLEPGATTVTAYFPAGRWFS 728

Query: 738 LFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVAVSN 797
           L+++S  VA K+G+++TL APAD +NVHV  GNIL L   A+T+   R++   LLVA+++
Sbjct: 729 LYDFSLAVATKTGKRVTLPAPADTVNVHVAGGNILTLQQPALTSSRVRQSVVHLLVALAD 788

Query: 798 GQSSFGEVFLDDGEVVEMGAEGGNWSLVRF--YSEVVGSKLLVKSQVINGGFALSQKLII 857
             ++ G++FLDDGE  EM      WS ++F   +E  G  + V+S V++  +A S+ + I
Sbjct: 789 DGTATGDLFLDDGESPEMAGPRSRWSQIKFSGATESGGGVVRVRSHVVHDSYAPSRTMAI 848

Query: 858 DKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTK--FMNIEISGLSIPIWEE 911
            KV  +G        G ++  + GV +  ++ +        K       +SGL++ + +E
Sbjct: 849 GKVVLMGLRSAAPPKGFAV-YANGVQVNASTAVGGAAGSPEKGALGVAHVSGLTLVVGQE 881

BLAST of HG10021486 vs. ExPASy Swiss-Prot
Match: Q43763 (Alpha-glucosidase OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 1.3e-277
Identity = 464/837 (55.44%), Postives = 609/837 (72.76%), Query Frame = 0

Query: 81  DLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRS-NSRIRSLPENHVGSPKASF 140
           D+ RL + A+ ET+ RLR+RITD+   RWE+P  IIPR +    +   P       +   
Sbjct: 59  DVQRLAVYASLETDSRLRVRITDADHPRWEVPQDIIPRPAPGDVLHDAPPASSAPLQGRV 118

Query: 141 ISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVFKDQYIQLSSSLPK 200
           +S   SDL+ T+H  +PF F+V RRS+GD LFDT+P        LVF+D+Y++++S+LP 
Sbjct: 119 LSPAGSDLVLTVH-ASPFRFTVSRRSTGDTLFDTAPG-------LVFRDKYLEVTSALPA 178

Query: 201 DRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYIDVRSPSRDGK 260
            R+S++G+GE T+ SF+L    N + TLWNADIG+  +DVNLYG+HPFY+DVR+P     
Sbjct: 179 GRASLYGLGEHTKSSFRL--RHNDSFTLWNADIGASYVDVNLYGSHPFYMDVRAP----- 238

Query: 261 VAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 320
              GT HGVLLL+SNGMD++Y G  VTYKVIGG++D YFFAGP+P++V+DQYT+LI RPA
Sbjct: 239 ---GTAHGVLLLSSNGMDVLYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPA 298

Query: 321 PVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKDFTFDPINFP 380
           P+PYWSFGFHQCRYGY NVSD+E VVARYAKA IPLEV+WTDIDYMD +KDFT D +NF 
Sbjct: 299 PMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFT 358

Query: 381 VEKMKKFVDNLHKNGQKYVLILDPGIST---NNTYGTYIRGKKADIFIKYNGVPYLGEVW 440
             +++ FVD LH+N QKYVLILDPGI     + TYGT++RG + DIF+K NG  ++G VW
Sbjct: 359 AAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKRNGTNFVGNVW 418

Query: 441 PGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFITSSTSPFSNLDNPPY 500
           PG VYFPDF+HP +  FW  EI LFR  +P DGLWIDMNEISNF   +  P + LD+PPY
Sbjct: 419 PGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFY--NPEPMNALDDPPY 478

Query: 501 MINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATHASLIKVTGKRPFVLSR 560
            IN+    RP+NNKTV    +H+G +TEY  HNL+G LE++AT   +++ TG+RPFVLSR
Sbjct: 479 RINNDGTGRPINNKTVRPLAVHYGGVTEYEEHNLFGLLEARATGRGVLRDTGRRPFVLSR 538

Query: 561 STFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGADICGFSGDTTEELCQR 620
           STFVGSG+YTA+WTGDN ATW DL YSI ++L+FGLFG+PM+GADICGF+G+TTEELC R
Sbjct: 539 STFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGR 598

Query: 621 WIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLLPYFYTLMYEAHKKGT 680
           WIQLGAFYPF+RDHS   ++R+ELYLW +VAAS RK L LRY+LLPYFYTLMYEAH  G 
Sbjct: 599 WIQLGAFYPFSRDHSAIFTVRRELYLWPSVAASGRKALGLRYQLLPYFYTLMYEAHMTGA 658

Query: 681 PIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVDAYFPAGNWFSLFNYSEL 740
           PIARPLFFS+P D+ TY ++ QFLLG GVLVSPVL+ G  +VDAYFPAG W+ L++YS  
Sbjct: 659 PIARPLFFSYPHDVATYGVDRQFLLGRGVLVSPVLEPGPTTVDAYFPAGRWYRLYDYSLA 718

Query: 741 VAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVAVSNGQSSFGE 800
           VA ++G+ + L APAD +NVH+  G IL L   A+TT  AR TA+ LLVA++   ++ G 
Sbjct: 719 VATRTGKHVRLPAPADTVNVHLTGGTILPLQQSALTTSRARRTAFHLLVALAEDGTASGY 778

Query: 801 VFLDDGEVVEMGAEGGNWSLVRFYSEVVGSK--LLVKSQVINGGFALSQKLIIDKVTFVG 860
           +FLDDG+  E G    +WS+VRF  ++  +K  + VKS+V++  +A S+ L+I KV  +G
Sbjct: 779 LFLDDGDSPEYGRR-SDWSMVRFNYKIPNNKGAIKVKSEVVHNSYAQSRTLVISKVVLMG 838

Query: 861 FERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNI-EISGLSIPIWEEFILEM 911
              P     L+++++    ++ +S+    YQ       +  I GLS+ + EEF L++
Sbjct: 839 HRSPAAPKKLTVHVN-SAEVEASSSAGTRYQNAGGLGGVAHIGGLSLVVGEEFELKV 873

BLAST of HG10021486 vs. ExPASy Swiss-Prot
Match: Q9S7Y7 (Alpha-xylosidase 1 OS=Arabidopsis thaliana OX=3702 GN=XYL1 PE=1 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 8.7e-237
Identity = 422/929 (45.43%), Postives = 596/929 (64.16%), Query Frame = 0

Query: 15  SLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVDPAGKSLTAHLDLIRT 74
           S   SL FS  LL  L    F P     S   +G GYR+ S    P G     +L + + 
Sbjct: 3   SSSSSLAFSLSLLLALIL-CFSP---TQSYKTIGKGYRLVSIEESPDG-GFIGYLQVKQK 62

Query: 75  SPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRSNSRIRSLPENHVGS 134
           + +YG D+  L L    ET+ RLR+ ITD+ ++RWE+P  ++PR    ++  +    +G 
Sbjct: 63  NKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKV----IGK 122

Query: 135 PKASFIS---DPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVFKDQYI 194
            + S I+      S+LIF+ + T PF F+V RRS+ + LF+T+       + LVFKDQY+
Sbjct: 123 SRKSPITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYL 182

Query: 195 QLSSSLPKDRSSIFGIGEQTR-ESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYID 254
           ++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP Y+D
Sbjct: 183 EISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPMYMD 242

Query: 255 VRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQ 314
           +R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V+DQ
Sbjct: 243 LRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQ 302

Query: 315 YTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKD 374
           YT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+V+W D D+MD +KD
Sbjct: 303 YTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKD 362

Query: 375 FTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFIKYNGVP 434
           FT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YGT+ R   AD+FIKY G P
Sbjct: 363 FTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKP 422

Query: 435 YLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFIT-------- 494
           +L +VWPG VYFPDFL+P + ++WG EI+ F D+VP DGLWIDMNE+SNF +        
Sbjct: 423 FLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEG 482

Query: 495 ----SSTSP------------FSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYN 554
               S   P             +  D+PPY IN+  V  P+  KT+  S  H+  + EY+
Sbjct: 483 KQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYD 542

Query: 555 THNLYGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPS 614
            H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTGDN  TW  L+ SI +
Sbjct: 543 AHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSIST 602

Query: 615 ILNFGLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTV 674
           +LNFG+FG+PMVG+DICGF    TEELC RWI++GAFYPF+RDH++  S RQELY WDTV
Sbjct: 603 MLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTV 662

Query: 675 AASARKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVL 734
           A SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y  + QFLLG   +
Sbjct: 663 ADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFM 722

Query: 735 VSPVLKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILAL 794
           +SPVL++G   V+A FP G+W+ +F+ ++ V  K+G+++TL AP + +NVH+ +  IL  
Sbjct: 723 ISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPT 782

Query: 795 HGEAMTTQAARETAYKLLVAVSNGQS---SFGEVFLDDGEVVEMGAEGGNWSLVRFYSEV 854
               + ++ AR T + L++A   G S   + G+++LD+ E+ EM    G  + V FY+ V
Sbjct: 783 QQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASV 842

Query: 855 VGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKT 907
               + + SQV  G FALS+  +I+KV+ +G     ++  + +N S        S+   T
Sbjct: 843 GNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHT 902

BLAST of HG10021486 vs. ExPASy TrEMBL
Match: A0A0A0M3Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G716250 PE=3 SV=1)

HSP 1 Score: 1661.4 bits (4301), Expect = 0.0e+00
Identity = 813/915 (88.85%), Postives = 857/915 (93.66%), Query Frame = 0

Query: 1   MASG-SKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVD 60
           MASG SK IRTPF  SL         +L L  F SF PLPAA SLPAVG GYRIRSSHVD
Sbjct: 15  MASGSSKVIRTPFPHSLP--------ILILFLFTSFLPLPAA-SLPAVGLGYRIRSSHVD 74

Query: 61  PAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRR 120
           PAGK+LTA LDLI TS VYGPDL  LTLQATFE++DRLR+RITDSTRERWE+P  I+PR 
Sbjct: 75  PAGKTLTADLDLIGTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEVPHHILPRP 134

Query: 121 SNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSD 180
           S+S IRSLPENHV SPKASFIS PASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSP FSD
Sbjct: 135 SSSLIRSLPENHVASPKASFISHPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPAFSD 194

Query: 181 PETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNLDV 240
            ETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS+NLDV
Sbjct: 195 SETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNLDV 254

Query: 241 NLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFF 300
           NLYGAHPFYID+RSPS+DGKVAAGTTHGVLLLNSNGMDI+YSGDR+TYKVIGGIIDLYFF
Sbjct: 255 NLYGAHPFYIDLRSPSQDGKVAAGTTHGVLLLNSNGMDIMYSGDRITYKVIGGIIDLYFF 314

Query: 301 AGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLW 360
           AGPSPISV+DQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLE +W
Sbjct: 315 AGPSPISVVDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEAMW 374

Query: 361 TDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKK 420
           TDIDYMD YKDFTFDPINFP +KMK FVDNLHKNGQKYVLILDPGISTNNTYG YIRG K
Sbjct: 375 TDIDYMDGYKDFTFDPINFPSQKMKIFVDNLHKNGQKYVLILDPGISTNNTYGPYIRGTK 434

Query: 421 ADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISN 480
           ADIF+KYNGVPYLG+VWPG VYFPDF HP+SE FWG EIQ+FRDIVPFDGLWIDMNEISN
Sbjct: 435 ADIFMKYNGVPYLGDVWPGPVYFPDFFHPDSETFWGREIQIFRDIVPFDGLWIDMNEISN 494

Query: 481 FITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKAT 540
           FITSSTSP SNLDNPPYMIN+ARV+RPLNNKTVPAS+LHFGNLTEYNTHNLYGFLES+AT
Sbjct: 495 FITSSTSPLSNLDNPPYMINNARVQRPLNNKTVPASILHFGNLTEYNTHNLYGFLESRAT 554

Query: 541 HASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVG 600
           HASL+KVTG+RPFVLSRSTFVGSGKYTAHWTGDNGATWNDL Y+IPSILNFGLFGIPMVG
Sbjct: 555 HASLVKVTGQRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLGYTIPSILNFGLFGIPMVG 614

Query: 601 ADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYR 660
           +DICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALRY+
Sbjct: 615 SDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALRYQ 674

Query: 661 LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVD 720
           LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKT+EI+SQFLLGGGVLVSPVLKEGA SVD
Sbjct: 675 LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTHEIDSQFLLGGGVLVSPVLKEGAFSVD 734

Query: 721 AYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARET 780
           AYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGEAMTT+AA+ET
Sbjct: 735 AYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTRAAQET 794

Query: 781 AYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVINGGF 840
            YKLLV +SNGQSSFGEVFLDDGEVVEMG EGGNWS+VRFYSE VGSKL+VKSQVINGGF
Sbjct: 795 PYKLLVVISNGQSSFGEVFLDDGEVVEMGREGGNWSMVRFYSETVGSKLVVKSQVINGGF 854

Query: 841 ALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISGLS 900
           ALSQKLIIDKVTFVGF+RPKKM  L LNISKG+NL GNS+IRKTYQYF KFMN+EISGLS
Sbjct: 855 ALSQKLIIDKVTFVGFKRPKKMVDLGLNISKGLNLNGNSSIRKTYQYFAKFMNVEISGLS 914

Query: 901 IPIWEEFILEMTPIS 915
           IPIWEEFILEMTPI+
Sbjct: 915 IPIWEEFILEMTPIN 920

BLAST of HG10021486 vs. ExPASy TrEMBL
Match: A0A5D3E1Q3 (Alpha-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00330 PE=3 SV=1)

HSP 1 Score: 1658.7 bits (4294), Expect = 0.0e+00
Identity = 813/917 (88.66%), Postives = 854/917 (93.13%), Query Frame = 0

Query: 1   MASGSK---SIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSH 60
           MASGS    +IRTPF+ SL        L+L L  F SF   P A SLPAVG+GYRIRSSH
Sbjct: 1   MASGSSKAITIRTPFAHSL--------LILILFLFTSFLIPPPAASLPAVGFGYRIRSSH 60

Query: 61  VDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIP 120
           +DPAGKSLTA L LIRTS VYGPDL  LTLQATFE++DRLR+RITDSTRERWEIPD IIP
Sbjct: 61  IDPAGKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIP 120

Query: 121 RRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDF 180
           R SNS IRSLPENHV SPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDF
Sbjct: 121 RPSNSLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDF 180

Query: 181 SDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNL 240
           S+ ETF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS+NL
Sbjct: 181 SNSETFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNL 240

Query: 241 DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLY 300
           DVNLYGAHPFYIDVRS SRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLY
Sbjct: 241 DVNLYGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLY 300

Query: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEV 360
           FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLEV
Sbjct: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEV 360

Query: 361 LWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRG 420
           +WTDIDYMD YKDFTFDPINFP EKMK FVDNLHKNGQKYV+ILDPGISTNNTYG YIRG
Sbjct: 361 MWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRG 420

Query: 421 KKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEI 480
            KADIF+K++GVPYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNEI
Sbjct: 421 TKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEI 480

Query: 481 SNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESK 540
           SNFITSSTSP SNLDNPPYMIN+ARVRRPLNNKTVPAS+LHFGNLTEYNTHNLYGFLES+
Sbjct: 481 SNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESR 540

Query: 541 ATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPM 600
           ATHASL+KVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+ Y+IPSILNFGLFGIPM
Sbjct: 541 ATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPM 600

Query: 601 VGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALR 660
           VG+DICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALR
Sbjct: 601 VGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALR 660

Query: 661 YRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVS 720
           Y+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPVLKEGA S
Sbjct: 661 YQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFS 720

Query: 721 VDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAAR 780
           VDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGEAMTTQAAR
Sbjct: 721 VDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAAR 780

Query: 781 ETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVING 840
           ETAYKLLV +SNGQ SFGEVFLDDGEV EMG EGGNWS+VRF SE VGSKL+VKSQVING
Sbjct: 781 ETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVING 840

Query: 841 GFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISG 900
           GFALSQKLIIDKVTFVGFERPKKM GL LNISKGV+L GNS+IRKTYQYF KFMN+EISG
Sbjct: 841 GFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISG 900

Query: 901 LSIPIWEEFILEMTPIS 915
           LSIPIWEEFILEMTPIS
Sbjct: 901 LSIPIWEEFILEMTPIS 909

BLAST of HG10021486 vs. ExPASy TrEMBL
Match: A0A1S3C8V0 (alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103498289 PE=3 SV=1)

HSP 1 Score: 1658.7 bits (4294), Expect = 0.0e+00
Identity = 813/917 (88.66%), Postives = 854/917 (93.13%), Query Frame = 0

Query: 1   MASGSK---SIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSH 60
           MASGS    +IRTPF+ SL        L+L L  F SF   P A SLPAVG+GYRIRSSH
Sbjct: 1   MASGSSKAITIRTPFAHSL--------LILILFLFTSFLIPPPAASLPAVGFGYRIRSSH 60

Query: 61  VDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIP 120
           +DPAGKSLTA L LIRTS VYGPDL  LTLQATFE++DRLR+RITDSTRERWEIPD IIP
Sbjct: 61  IDPAGKSLTADLQLIRTSQVYGPDLPTLTLQATFESKDRLRVRITDSTRERWEIPDHIIP 120

Query: 121 RRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDF 180
           R SNS IRSLPENHV SPK SFISDPASDLIFTL+DTAPFGFSVLRRSSGDVLFDTSPDF
Sbjct: 121 RPSNSLIRSLPENHVASPKPSFISDPASDLIFTLYDTAPFGFSVLRRSSGDVLFDTSPDF 180

Query: 181 SDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNADIGSLNL 240
           S+ ETF+VFKDQYIQLSS LPKDRSSIFGIGEQTR+SFKLVPDKNKTLTLWNADIGS+NL
Sbjct: 181 SNSETFIVFKDQYIQLSSLLPKDRSSIFGIGEQTRKSFKLVPDKNKTLTLWNADIGSVNL 240

Query: 241 DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLY 300
           DVNLYGAHPFYIDVRS SRDGKV AGTTHGVLLLNSNGMDIIYSGDR+TYKVIGGIIDLY
Sbjct: 241 DVNLYGAHPFYIDVRSRSRDGKVVAGTTHGVLLLNSNGMDIIYSGDRITYKVIGGIIDLY 300

Query: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEV 360
           FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVS++ESVVARYAKASIPLEV
Sbjct: 301 FFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSEVESVVARYAKASIPLEV 360

Query: 361 LWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRG 420
           +WTDIDYMD YKDFTFDPINFP EKMK FVDNLHKNGQKYV+ILDPGISTNNTYG YIRG
Sbjct: 361 MWTDIDYMDGYKDFTFDPINFPSEKMKIFVDNLHKNGQKYVIILDPGISTNNTYGPYIRG 420

Query: 421 KKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEI 480
            KADIF+K++GVPYLG+VWPG VYFPDFLHP+SE FWG EIQ+FRDIVPFDGLWIDMNEI
Sbjct: 421 TKADIFMKHDGVPYLGDVWPGPVYFPDFLHPDSETFWGREIQIFRDIVPFDGLWIDMNEI 480

Query: 481 SNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESK 540
           SNFITSSTSP SNLDNPPYMIN+ARVRRPLNNKTVPAS+LHFGNLTEYNTHNLYGFLES+
Sbjct: 481 SNFITSSTSPLSNLDNPPYMINNARVRRPLNNKTVPASILHFGNLTEYNTHNLYGFLESR 540

Query: 541 ATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPM 600
           ATHASL+KVTGKRPFVLSRSTF GSGKYTAHWTGD GATWND+ Y+IPSILNFGLFGIPM
Sbjct: 541 ATHASLVKVTGKRPFVLSRSTFAGSGKYTAHWTGDIGATWNDMGYTIPSILNFGLFGIPM 600

Query: 601 VGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALR 660
           VG+DICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWD+VAASARKVLALR
Sbjct: 601 VGSDICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDSVAASARKVLALR 660

Query: 661 YRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVS 720
           Y+LLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEI+SQFLLG GVLVSPVLKEGA S
Sbjct: 661 YQLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEIDSQFLLGEGVLVSPVLKEGAFS 720

Query: 721 VDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAAR 780
           VDAYFPAGNWFSLFNYSE VAV SGQQI LDAPADHINVHVREGNILALHGEAMTTQAAR
Sbjct: 721 VDAYFPAGNWFSLFNYSEFVAVNSGQQINLDAPADHINVHVREGNILALHGEAMTTQAAR 780

Query: 781 ETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVING 840
           ETAYKLLV +SNGQ SFGEVFLDDGEV EMG EGGNWS+VRF SE VGSKL+VKSQVING
Sbjct: 781 ETAYKLLVVISNGQRSFGEVFLDDGEVAEMGGEGGNWSMVRFCSETVGSKLVVKSQVING 840

Query: 841 GFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKFMNIEISG 900
           GFALSQKLIIDKVTFVGFERPKKM GL LNISKGV+L GNS+IRKTYQYF KFMN+EISG
Sbjct: 841 GFALSQKLIIDKVTFVGFERPKKMVGLGLNISKGVSLNGNSSIRKTYQYFAKFMNVEISG 900

Query: 901 LSIPIWEEFILEMTPIS 915
           LSIPIWEEFILEMTPIS
Sbjct: 901 LSIPIWEEFILEMTPIS 909

BLAST of HG10021486 vs. ExPASy TrEMBL
Match: A0A6J1KFK1 (alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111495351 PE=3 SV=1)

HSP 1 Score: 1613.6 bits (4177), Expect = 0.0e+00
Identity = 794/924 (85.93%), Postives = 851/924 (92.10%), Query Frame = 0

Query: 1   MASGSKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLP----------AVGYG 60
           MA+GSKS++      L+   L SA++LFL  F  FF L A  SLP          AVGYG
Sbjct: 22  MATGSKSLQA----FLRRRRLLSAVVLFL--FSCFFRLIA--SLPFAAGEQRENSAVGYG 81

Query: 61  YRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWE 120
           YR+RS  VDP GKSLTA LDLI  S VYGPD+ RL+LQA+FET+DRLR+RITDS RERWE
Sbjct: 82  YRVRSGRVDPTGKSLTADLDLIEKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWE 141

Query: 121 IPDQIIPRRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVL 180
           IPD+IIPRRSNSRIRSLPE  VGSP+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVL
Sbjct: 142 IPDEIIPRRSNSRIRSLPERDVGSPETSIISDPASDLIFSLHDTAPFGFSVHRRSSGDVL 201

Query: 181 FDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNA 240
           FDTSP+FSD ETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFK+VPDK+KTLTLW+A
Sbjct: 202 FDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKIVPDKSKTLTLWSA 261

Query: 241 DIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVI 300
           DIGS+N DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDR++YK I
Sbjct: 262 DIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRISYKAI 321

Query: 301 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAK 360
           GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYAK
Sbjct: 322 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAK 381

Query: 361 ASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNT 420
           A IPLEV+WTDIDYMD YKDFTFDPINFPVEKMK FVD+LH+NGQKYVLI+DPGISTN T
Sbjct: 382 AGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDSLHQNGQKYVLIVDPGISTNKT 441

Query: 421 YGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGL 480
           YGTYIRG +ADIFIKY+GVPYLGEVWPG VYFPDFLHPNSE FWGGEI+LFRDIVPFDGL
Sbjct: 442 YGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGGEIKLFRDIVPFDGL 501

Query: 481 WIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNL 540
           WIDMNE+SNFITSSTSPFSNLDNPPY I++A V+RP+NN+TVPAS LHFGNLTEYNTHNL
Sbjct: 502 WIDMNELSNFITSSTSPFSNLDNPPYKIDNAGVQRPINNRTVPASSLHFGNLTEYNTHNL 561

Query: 541 YGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNF 600
           YGFLESKATHASL+KVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDL Y+IPSILNF
Sbjct: 562 YGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNF 621

Query: 601 GLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 660
           GLFGIPMVGADICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA
Sbjct: 622 GLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 681

Query: 661 RKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPV 720
           RKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVLVSPV
Sbjct: 682 RKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGEGVLVSPV 741

Query: 721 LKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEA 780
           LKEGA+SVDAYFPAGNWFSLFNYSE VAVKSGQQITLDAPADHINVHVREGNILALHGEA
Sbjct: 742 LKEGALSVDAYFPAGNWFSLFNYSESVAVKSGQQITLDAPADHINVHVREGNILALHGEA 801

Query: 781 MTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLV 840
            TTQAAR+TA+KLLV VSNGQSS GEVFLDDGEVVEMG EGGNWSLVRFYSE VGSKLL+
Sbjct: 802 TTTQAARKTAFKLLVVVSNGQSSSGEVFLDDGEVVEMGEEGGNWSLVRFYSEAVGSKLLI 861

Query: 841 KSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKF 900
           KSQVINGGFALSQK+IIDKVTFVGFERPKKMGGL L+ISKG NL GNS IRKTY+Y  KF
Sbjct: 862 KSQVINGGFALSQKMIIDKVTFVGFERPKKMGGLGLDISKGANLNGNSGIRKTYEYSAKF 921

Query: 901 MNIEISGLSIPIWEEFILEMTPIS 915
           +N+EISGLSIPI EEF++E++P++
Sbjct: 922 VNVEISGLSIPICEEFVMELSPLN 937

BLAST of HG10021486 vs. ExPASy TrEMBL
Match: A0A6J1ENT6 (alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111434234 PE=3 SV=1)

HSP 1 Score: 1590.5 bits (4117), Expect = 0.0e+00
Identity = 783/924 (84.74%), Postives = 840/924 (90.91%), Query Frame = 0

Query: 1   MASGSKSIRTPFSQSLQPSLLFSALLLFLLYFPSFFPLPAANSLP----------AVGYG 60
           MA+GSKS++      L+   L SA++LFL  F  FF L A  SLP          AVGYG
Sbjct: 1   MATGSKSLQA----FLRRRRLLSAVVLFL--FTCFFRLTA--SLPFAAGEQRENSAVGYG 60

Query: 61  YRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWE 120
           YR+RS  VDP GKSLTA LDLI  S VYGPD+ RL+LQA+FET+DRLR+RITDS RERWE
Sbjct: 61  YRVRSGRVDPTGKSLTADLDLIDKSSVYGPDVERLSLQASFETKDRLRVRITDSNRERWE 120

Query: 121 IPDQIIPRRSNSRIRSLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRRSSGDVL 180
           +PD+IIPRRSNSRIRSLPE  VGSP+ S ISDPASDLIF+LHDTAPFGFSV RRSSGDVL
Sbjct: 121 VPDEIIPRRSNSRIRSLPERDVGSPETSVISDPASDLIFSLHDTAPFGFSVHRRSSGDVL 180

Query: 181 FDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKTLTLWNA 240
           FDTSP+FSD ETFLVFKDQYIQLSSSLPKDRSS+FGIGEQTR+SFKLVPDK+KTLTLWNA
Sbjct: 181 FDTSPNFSDSETFLVFKDQYIQLSSSLPKDRSSLFGIGEQTRKSFKLVPDKSKTLTLWNA 240

Query: 241 DIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVI 300
           DIGS+N DVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIY+GDR++YK I
Sbjct: 241 DIGSVNTDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYTGDRISYKAI 300

Query: 301 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAK 360
           GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIE+VVARYAK
Sbjct: 301 GGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIETVVARYAK 360

Query: 361 ASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNT 420
           A IPLEV+WTDIDYMD YKDFTFDPINFPVEKMK FVDNLH+NGQKYVLILDPGISTN T
Sbjct: 361 AGIPLEVMWTDIDYMDGYKDFTFDPINFPVEKMKNFVDNLHQNGQKYVLILDPGISTNKT 420

Query: 421 YGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGL 480
           YGTYIRG +ADIFIKY+GVPYLGEVWPG VYFPDFLHPNSE FWG EI+L RDIVPFDGL
Sbjct: 421 YGTYIRGIEADIFIKYDGVPYLGEVWPGPVYFPDFLHPNSEIFWGDEIKLLRDIVPFDGL 480

Query: 481 WIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNL 540
           WIDMNEISNFITSSTS FSNLDNPPY IN+A V+RP+NN+TVPAS LHFGNLTEYNTHNL
Sbjct: 481 WIDMNEISNFITSSTSSFSNLDNPPYKINNAGVQRPINNRTVPASSLHFGNLTEYNTHNL 540

Query: 541 YGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNF 600
           YGFLESKATHASL+KVTGKRPFVLSRSTFVGSGKYTAHWTGDN ATWNDL Y+IPSILNF
Sbjct: 541 YGFLESKATHASLVKVTGKRPFVLSRSTFVGSGKYTAHWTGDNAATWNDLGYTIPSILNF 600

Query: 601 GLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 660
           GLFGIPMVGADICGFSGDTTEELC+RWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA
Sbjct: 601 GLFGIPMVGADICGFSGDTTEELCRRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASA 660

Query: 661 RKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPV 720
           RKVLALRY LLPY YTLMYEAHKKGTPIARPLFFSFPQDI+TYEINSQFLLG GVL+SPV
Sbjct: 661 RKVLALRYSLLPYIYTLMYEAHKKGTPIARPLFFSFPQDIRTYEINSQFLLGAGVLISPV 720

Query: 721 LKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALHGEA 780
           LKEGA+SVDAYFPAGNWFSLFNYSE VA+KSGQQITLDAPADHINVHVREGNILALHGEA
Sbjct: 721 LKEGAISVDAYFPAGNWFSLFNYSESVAIKSGQQITLDAPADHINVHVREGNILALHGEA 780

Query: 781 MTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLV 840
            TTQAAR+T +KLLV VSNGQSS GEVFLDDGE +EMG EGGNWSLVRFYSE VGSKLL+
Sbjct: 781 TTTQAARKTEFKLLVVVSNGQSSSGEVFLDDGEEMEMGEEGGNWSLVRFYSEAVGSKLLI 840

Query: 841 KSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKTYQYFTKF 900
           KSQVINGGFALSQK+IIDKVT VGFERPK MG L L+ISKG NL GNS IR TY+Y  KF
Sbjct: 841 KSQVINGGFALSQKMIIDKVTIVGFERPKNMGDLGLDISKGANLNGNSGIRITYEYSAKF 900

Query: 901 MNIEISGLSIPIWEEFILEMTPIS 915
           +N++ISGLSIPI E F++E++ ++
Sbjct: 901 VNVQISGLSIPICEGFVMELSSLN 916

BLAST of HG10021486 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 560/869 (64.44%), Postives = 689/869 (79.29%), Query Frame = 0

Query: 47  VGYGYRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDLLRLTLQATFETEDRLRIRITDSTR 106
           VGYGY +RS  VD   + LTA LDLI+ S VY PD+  L L  + ET +RLRIRITDS++
Sbjct: 35  VGYGYVVRSVGVDSNRQVLTAKLDLIKPSSVYAPDIKSLNLHVSLETSERLRIRITDSSQ 94

Query: 107 ERWEIPDQIIPRRSNSRIR--SLPENHVGSPKASFISDPASDLIFTLHDTAPFGFSVLRR 166
           +RWEIP+ +IPR  N   R  S  E+   SP+ +F++DP+SDL+FTLH+T PFGFSV RR
Sbjct: 95  QRWEIPETVIPRAGNHSPRRFSTEEDGGNSPENNFLADPSSDLVFTLHNTTPFGFSVSRR 154

Query: 167 SSGDVLFDTSPDFSDPETFLVFKDQYIQLSSSLPKDRSSIFGIGEQTRESFKLVPDKNKT 226
           SSGD+LFDTSPD SD  T+ +FKDQ++QLSS+LP++RS+++GIGE T+ SF+L+P   +T
Sbjct: 155 SSGDILFDTSPDSSDSNTYFIFKDQFLQLSSALPENRSNLYGIGEHTKRSFRLIP--GET 214

Query: 227 LTLWNADIGSLNLDVNLYGAHPFYIDVRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDR 286
           +TLWNADIGS N DVNLYG+HPFY+DVR     G   AGTTHGVLLLNSNGMD+ Y G R
Sbjct: 215 MTLWNADIGSENPDVNLYGSHPFYMDVRGSK--GNEEAGTTHGVLLLNSNGMDVKYEGHR 274

Query: 287 VTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESV 346
           +TY VIGG+IDLY FAGPSP  V++QYTELIGRPAP+PYWSFGFHQCRYGYKNVSD+E V
Sbjct: 275 ITYNVIGGVIDLYVFAGPSPEMVMNQYTELIGRPAPMPYWSFGFHQCRYGYKNVSDLEYV 334

Query: 347 VARYAKASIPLEVLWTDIDYMDEYKDFTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPG 406
           V  YAKA IPLEV+WTDIDYMD YKDFT DP+NFP +KM+ FVD LHKNGQKYVLILDPG
Sbjct: 335 VDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPEDKMQSFVDTLHKNGQKYVLILDPG 394

Query: 407 ISTNNTYGTYIRGKKADIFIKYNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDI 466
           I  +++YGTY RG +AD+FIK NG PYLGEVWPG VYFPDFL+P +  FW  EI++F++I
Sbjct: 395 IGVDSSYGTYNRGMEADVFIKRNGEPYLGEVWPGKVYFPDFLNPAAATFWSNEIKMFQEI 454

Query: 467 VPFDGLWIDMNEISNFITSSTSPFSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTE 526
           +P DGLWIDMNE+SNFITS  S  S+LD+PPY IN++  +RP+NNKTVPA+ +HFGN++E
Sbjct: 455 LPLDGLWIDMNELSNFITSPLSSGSSLDDPPYKINNSGDKRPINNKTVPATSIHFGNISE 514

Query: 527 YNTHNLYGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSI 586
           Y+ HNLYG LE+KATH +++ +TGKRPF+LSRSTFV SGKYTAHWTGDN A W DL YSI
Sbjct: 515 YDAHNLYGLLEAKATHQAVVDITGKRPFILSRSTFVSSGKYTAHWTGDNAAKWEDLAYSI 574

Query: 587 PSILNFGLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWD 646
           P ILNFGLFGIPMVGADICGFS DTTEELC+RWIQLGAFYPFARDHS  G+ RQELYLWD
Sbjct: 575 PGILNFGLFGIPMVGADICGFSHDTTEELCRRWIQLGAFYPFARDHSSLGTARQELYLWD 634

Query: 647 TVAASARKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGG 706
           +VA+SARKVL LR RLLP+ YTLMYEAH  G PIARPLFFSFPQD KTYEI+SQFL+G  
Sbjct: 635 SVASSARKVLGLRMRLLPHLYTLMYEAHVSGNPIARPLFFSFPQDTKTYEIDSQFLIGKS 694

Query: 707 VLVSPVLKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNIL 766
           ++VSP LK+GAV+VDAYFPAGNWF LFNYS  V   SG+ + LD PADH+NVHVREG+I+
Sbjct: 695 IMVSPALKQGAVAVDAYFPAGNWFDLFNYSFAVGGDSGKHVRLDTPADHVNVHVREGSIV 754

Query: 767 ALHGEAMTTQAARETAYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGN--WSLVRFYSE 826
           A+ GEA+TT+ AR+T Y+LLV  S  ++  GE+FLDDGE + MGA GGN  W+LV+F   
Sbjct: 755 AMQGEALTTRDARKTPYQLLVVASRLENISGELFLDDGENLRMGAGGGNRDWTLVKFRCY 814

Query: 827 VVGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRK 886
           V G  ++++S+V+N  +A   K  I KVTFVGFE  + +    +  S+ +     S I+ 
Sbjct: 815 VTGKSVVLRSEVVNPEYASKMKWSIGKVTFVGFENVENVKTYEVRTSERLRSPRISLIKT 874

Query: 887 TYQY-FTKFMNIEISGLSIPIWEEFILEM 911
                  +F+++E+S LS+ + ++F + +
Sbjct: 875 VSDNDDPRFLSVEVSKLSLLVGKKFEMRL 899

BLAST of HG10021486 vs. TAIR 10
Match: AT1G68560.1 (alpha-xylosidase 1 )

HSP 1 Score: 821.6 bits (2121), Expect = 6.2e-238
Identity = 422/929 (45.43%), Postives = 596/929 (64.16%), Query Frame = 0

Query: 15  SLQPSLLFSALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVDPAGKSLTAHLDLIRT 74
           S   SL FS  LL  L    F P     S   +G GYR+ S    P G     +L + + 
Sbjct: 3   SSSSSLAFSLSLLLALIL-CFSP---TQSYKTIGKGYRLVSIEESPDG-GFIGYLQVKQK 62

Query: 75  SPVYGPDLLRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRSNSRIRSLPENHVGS 134
           + +YG D+  L L    ET+ RLR+ ITD+ ++RWE+P  ++PR    ++  +    +G 
Sbjct: 63  NKIYGSDITTLRLFVKHETDSRLRVHITDAKQQRWEVPYNLLPREQPPQVGKV----IGK 122

Query: 135 PKASFIS---DPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETFLVFKDQYI 194
            + S I+      S+LIF+ + T PF F+V RRS+ + LF+T+       + LVFKDQY+
Sbjct: 123 SRKSPITVQEISGSELIFS-YTTDPFTFAVKRRSNHETLFNTT-------SSLVFKDQYL 182

Query: 195 QLSSSLPKDRSSIFGIGEQTR-ESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYID 254
           ++S+SLPK+ +S++G+GE ++    KLVP  N+  TL+  D+ ++NL+ +LYG+HP Y+D
Sbjct: 183 EISTSLPKE-ASLYGLGENSQANGIKLVP--NEPYTLYTEDVSAINLNTDLYGSHPMYMD 242

Query: 255 VRSPSRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQ 314
           +R+    GK  A   H VLLLNSNGMD+ Y GD +TYKVIGG+ D YF AGPSP++V+DQ
Sbjct: 243 LRNVG--GKAYA---HAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFYFIAGPSPLNVVDQ 302

Query: 315 YTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKD 374
           YT+LIGRPAP+PYWS GFHQCR+GY N+S +E VV  Y KA IPL+V+W D D+MD +KD
Sbjct: 303 YTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDVIWNDDDHMDGHKD 362

Query: 375 FTFDPINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFIKYNGVP 434
           FT +P+ +P  K+  F+D +HK G KY++I DPGI  N +YGT+ R   AD+FIKY G P
Sbjct: 363 FTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRAMAADVFIKYEGKP 422

Query: 435 YLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFIT-------- 494
           +L +VWPG VYFPDFL+P + ++WG EI+ F D+VP DGLWIDMNE+SNF +        
Sbjct: 423 FLAQVWPGPVYFPDFLNPKTVSWWGDEIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPEG 482

Query: 495 ----SSTSP------------FSNLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYN 554
               S   P             +  D+PPY IN+  V  P+  KT+  S  H+  + EY+
Sbjct: 483 KQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFKTIATSATHYNGVREYD 542

Query: 555 THNLYGFLESKATHASLIKVTGKRPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPS 614
            H++YGF E+ ATH  L+ V GKRPF+LSRSTFVGSG+Y AHWTGDN  TW  L+ SI +
Sbjct: 543 AHSIYGFSETIATHKGLLNVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSIST 602

Query: 615 ILNFGLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLWDTV 674
           +LNFG+FG+PMVG+DICGF    TEELC RWI++GAFYPF+RDH++  S RQELY WDTV
Sbjct: 603 MLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSPRQELYQWDTV 662

Query: 675 AASARKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVL 734
           A SAR  L +RY++LP+ YTL YEAH  G PIARPLFFSFP+  + Y  + QFLLG   +
Sbjct: 663 ADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFPEYTECYGNSRQFLLGSSFM 722

Query: 735 VSPVLKEGAVSVDAYFPAGNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILAL 794
           +SPVL++G   V+A FP G+W+ +F+ ++ V  K+G+++TL AP + +NVH+ +  IL  
Sbjct: 723 ISPVLEQGKTEVEALFPPGSWYHMFDMTQAVVSKNGKRVTLPAPLNFVNVHLYQNTILPT 782

Query: 795 HGEAMTTQAARETAYKLLVAVSNGQS---SFGEVFLDDGEVVEMGAEGGNWSLVRFYSEV 854
               + ++ AR T + L++A   G S   + G+++LD+ E+ EM    G  + V FY+ V
Sbjct: 783 QQGGLISKDARTTPFSLVIAFPAGASEGYATGKLYLDEDELPEMKLGNGQSTYVDFYASV 842

Query: 855 VGSKLLVKSQVINGGFALSQKLIIDKVTFVGFERPKKMGGLSLNISKGVNLKGNSTIRKT 907
               + + SQV  G FALS+  +I+KV+ +G     ++  + +N S        S+   T
Sbjct: 843 GNGTMKMWSQVKEGKFALSKGWVIEKVSVLGLRGAGQVSEIQINGSPMTKKIEVSSKEHT 902

BLAST of HG10021486 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 815.1 bits (2104), Expect = 5.8e-236
Identity = 418/901 (46.39%), Postives = 575/901 (63.82%), Query Frame = 0

Query: 23  SALLLFLLYFPSFFPLPAANSLPAVGYGYRIRSSHVDPAGKSLTAHLDLIRTSPVYGPDL 82
           S L L +     F  L  +N   A+G GYR+ S    P   S   +L + +++ +YG D+
Sbjct: 3   SCLSLLVAIILCFSSLQCSN---AIGKGYRLISMEKSPDDGSFIGYLQVKQSNKIYGSDI 62

Query: 83  LRLTLQATFETEDRLRIRITDSTRERWEIPDQIIPRRSNSRIRSLPENHVGSPKAS---- 142
             L L   + T+ RLR+ ITD+ ++RWE+P  ++ R         P N +G  + S    
Sbjct: 63  TILRLFINYRTDHRLRVHITDAKKQRWEVPYNLLRREQ-------PPNVIGKSRKSPVTV 122

Query: 143 -FISDPASDLIFTLHDTAPFGFSVLRRSSGDVLFDTSPDFSDPETF--LVFKDQYIQLSS 202
             IS P   LIFT+    PF F+V RRS+G+ +F+TS   S  E+F  +VFKDQY+++S+
Sbjct: 123 QEISGPELILIFTVD---PFSFAVRRRSNGETIFNTS---SSDESFGEMVFKDQYLEIST 182

Query: 203 SLPKDRSSIFGIGEQTR-ESFKLVPDKNKTLTLWNADIGSLNLDVNLYGAHPFYIDVRSP 262
           SLPKD +S++G GE ++    KLVP  N+  TL+  D+ + NL+ +LYG+HP Y+D+R+ 
Sbjct: 183 SLPKD-ASLYGFGENSQANGIKLVP--NEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNV 242

Query: 263 SRDGKVAAGTTHGVLLLNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTEL 322
           S  GK  A   H VLLLNS+GMD+ Y GD +TYKVIGG+ D YFFAGPSP++V+DQYT L
Sbjct: 243 S--GKAYA---HSVLLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPLNVVDQYTSL 302

Query: 323 IGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKDFTFD 382
           IGRPAP+PYWS GFHQCR+GY+NVS ++ VV  Y KA IPL+V+W D DYMD YKDFT D
Sbjct: 303 IGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYMDGYKDFTLD 362

Query: 383 PINFPVEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFIKYNGVPYLGE 442
            +NFP  K+  F+D +HK G KYV+I DPGI  N +YG Y RG  +D+FIKY G P+L +
Sbjct: 363 LVNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIKYEGKPFLAQ 422

Query: 443 VWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNFITSSTSPFSNLDNP 502
           VWPG VYFPDFL+P + ++WG EI+ F ++VP DGLWIDMNE                  
Sbjct: 423 VWPGPVYFPDFLNPKTVSWWGDEIRRFHELVPIDGLWIDMNE------------------ 482

Query: 503 PYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATHASLIKVTGKRPFVL 562
              IN+   +  L  KT+P S  H+  + EY+ H++YGF E+ ATH +L+ V GKRPF+L
Sbjct: 483 ---INATGHKASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALLAVQGKRPFIL 542

Query: 563 SRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGADICGFSGDTTEELC 622
           SRSTFVGSG+Y AHWTGDN  TW  L+ SI ++LNFG+FG+PMVG+DICGF   T EELC
Sbjct: 543 SRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELC 602

Query: 623 QRWIQLGAFYPFARDHSDKGSIRQELYLWDTVAASARKVLALRYRLLPYFYTLMYEAHKK 682
            RWI++GAFYPF+RDH+D  + R+ELY W TVA SAR  L +RY+LLP+ YTL YEAH  
Sbjct: 603 NRWIEVGAFYPFSRDHADYYAPRKELYQWGTVAESARNALGMRYKLLPFLYTLNYEAHMS 662

Query: 683 GTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVLKEGAVSVDAYFPAGNWFSLFNYS 742
           G PIARPLFFSFP+  + Y ++ QFLLG  +++SPVL++G   V+A FP G+W+ +F+ +
Sbjct: 663 GAPIARPLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFPPGSWYHMFDMT 722

Query: 743 ELVAVKSGQQITLDAPADHINVHVREGNILALHGEAMTTQAARETAYKLLVAVSNGQSSF 802
           ++V  K+G+  TL AP + +NVH+ +  IL +           +         S G +S 
Sbjct: 723 QVVVSKNGRLFTLPAPFNVVNVHLYQNAILPM-----------QQVVAFPAGASEGYAS- 782

Query: 803 GEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVINGGFALSQKLIIDKVTFVG 862
           G++FLDD E+ EM    G  + + FY+ V    + + SQV  G FALSQ L+I+KV  +G
Sbjct: 783 GKLFLDDDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLG 842

Query: 863 FERPKKMGGLSLNISKGVNLKGNSTIRKTYQYF---------TKFMNIEISGLSIPIWEE 907
            +   K+  + LN S   N      +    Q +         +K   +E+ GL + + ++
Sbjct: 843 LKGTWKVSEILLNGSSISNETKTIEVSSKEQMYVVGSEDEGESKSFMVELKGLEMLVGKD 846

BLAST of HG10021486 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 334.3 bits (856), Expect = 3.0e-91
Identity = 223/688 (32.41%), Postives = 337/688 (48.98%), Query Frame = 0

Query: 200 DRSSIFGIGEQTRESFKLVPDK------NKTLTLWNADIGSLNLD--VNLYGAHPFYIDV 259
           D S ++GI E    SF L P K      ++   L+N D+   + +    LYG+ PF +  
Sbjct: 222 DSSFVYGIPEHA-TSFALKPTKGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVS- 281

Query: 260 RSPSRDGKVAAGTTHGVLLLNS---------------NGMDIIYSGDRVT--YKVIGGII 319
                 GK  +G T G   LN+               +G+ +  S  R+   +    GI+
Sbjct: 282 -----HGK--SGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIV 341

Query: 320 DLYFFAGPSPISVIDQYTELIGRPAPVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIP 379
           D +FF GP P  V+ QY  + G  A    ++ G+HQCR+ YK+  D+  V +++ +  IP
Sbjct: 342 DTFFFVGPEPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIP 401

Query: 380 LEVLWTDIDYMDEYKDFTFDPINFP-VEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGT 439
            +VLW DI++ D  + FT+D + FP  E+M+K    L   G+K V I+DP I  +++Y  
Sbjct: 402 YDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQK---KLAAKGRKMVTIVDPHIKRDDSYFL 461

Query: 440 YIRGKKADIFIK-YNGVPYLGEVWPGLVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWI 499
           +    +   ++K  +G  + G  WPG   + D L P    +WGG                
Sbjct: 462 HKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRF-------------- 521

Query: 500 DMNEISNFITSSTSPFS-NLDNPPYMINSARVRRPLNNKTVPASVLHFGNLTEYNTHNLY 559
                 N++ S+ S ++ N  N P + N   V       T+P   LH G +     HN Y
Sbjct: 522 ---SYKNYVGSTPSLYTWNDMNEPSVFNGPEV-------TMPRDALHVGGVEHREVHNAY 581

Query: 560 GFLESKATHASLI-KVTGK-RPFVLSRSTFVGSGKYTAHWTGDNGATWNDLEYSIPSILN 619
           G+    AT   L+ +  GK RPFVLSR+ F G+ +Y A WTGDN A W  L  SIP IL 
Sbjct: 582 GYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMILT 641

Query: 620 FGLFGIPMVGADICGFSGDTTEELCQRWIQLGAFYPFARDHSDKGSIRQELYLW-DTVAA 679
            GL GI   GADI GF G+   EL  RW Q+GA+YPF R H+   + R+E +L+ +    
Sbjct: 642 LGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNTE 701

Query: 680 SARKVLALRYRLLPYFYTLMYEAHKKGTPIARPLFFSFPQDIKTYEINSQFLLGGGVLVS 739
             R  +  RY LLPYFYTL  EA+  G P+ RPL+  FPQD  T+  +  F++G G+LV 
Sbjct: 702 LMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLLVQ 761

Query: 740 PVLKEGAVSVDAYFPA-GNWFSLFNYSELVAVKSGQQITLDAPADHINVHVREGNILALH 799
            V  +G      Y P   +W+ L N    V    G+   +DAP + I    + G I+   
Sbjct: 762 GVYTKGTTQASVYLPGKESWYDLRNGKTYV---GGKTHKMDAPEESIPAFQKAGTIIPRK 821

Query: 800 GEAMTTQAARET-AYKLLVAVSNGQSSFGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGS 855
                + +  +   Y L+VA+++ Q + GE+++DDG+  E     G++   RF   V   
Sbjct: 822 DRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEF--RRGSYIHRRF---VFSK 865

BLAST of HG10021486 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 303.9 bits (777), Expect = 4.3e-82
Identity = 199/644 (30.90%), Postives = 316/644 (49.07%), Query Frame = 0

Query: 202 SSIFGIGEQTRESFKLVPDKNKTLTLWNADI-GSLNLDVNLYGAHPFYIDVRSPSRDGKV 261
           +S +G GE + +    +    K +  WN D  G  +   +LY +HP+ + V        V
Sbjct: 86  TSFYGTGEVSGQ----LERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGV 145

Query: 262 AAGTTHGVLL-LNSNGMDIIYSGDRVTYKVIGGIIDLYFFAGPSPISVIDQYTELIGRPA 321
            A TT    + L   G+  I S    +Y +I       F    SP +V++  +  IG   
Sbjct: 146 LADTTRKCEIDLRKEGIIRIIS--PASYPII------TFGPFSSPTAVLESLSHAIGTVF 205

Query: 322 PVPYWSFGFHQCRYGYKNVSDIESVVARYAKASIPLEVLWTDIDYMDEYKDFTFDPINFP 381
             P W+ G+HQCR+ Y +   +  +   +    IP +V+W DIDYMD ++ FTFD   FP
Sbjct: 206 MPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFP 265

Query: 382 VEKMKKFVDNLHKNGQKYVLILDPGISTNNTYGTYIRGKKADIFI-KYNGVPYLGEVWPG 441
                    +LH NG K + +LDPGI     Y  Y  G K D++I + +G P+ GEVWPG
Sbjct: 266 --DPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFTGEVWPG 325

Query: 442 LVYFPDFLHPNSENFWGGEIQLFRDIVPFDGLWIDMNEISNF-ITSSTSPFSNLDNPPYM 501
              FPD+ +  + ++W   ++ F      DG+W DMNE + F + + T P +N+ +    
Sbjct: 326 PCVFPDYTNSKARSWWANLVKEFVS-NGVDGIWNDMNEPAVFKVVTKTMPENNIHHGDDE 385

Query: 502 INSARVRRPLNNKTVPASVLHFGNLTEYNTHNLYGFLESKATHASL-IKVTGKRPFVLSR 561
           +   +                  N + Y  HN+YG L +++T+  + +    KRPFVL+R
Sbjct: 386 LGGVQ------------------NHSHY--HNVYGMLMARSTYEGMELADKNKRPFVLTR 445

Query: 562 STFVGSGKYTAHWTGDNGATWNDLEYSIPSILNFGLFGIPMVGADICGFSGDTTEELCQR 621
           + F+GS +Y A WTGDN + W  L  SI  +L  GL G P+ G DI GF+G+ T  L  R
Sbjct: 446 AGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGR 505

Query: 622 WIQLGAFYPFARDHSDKGSIRQELYLW-DTVAASARKVLALRYRLLPYFYTLMYEAHKKG 681
           W+ +GA +PF R HS+ G+   E + + +      R  L  RY+LLP+FYTL Y AH  G
Sbjct: 506 WMGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTG 565

Query: 682 TPIARPLFFSFPQDIKTYEINSQFLLGGGVLVSPVL-KEGAVSVDAYFPAGNWFSLFNYS 741
            P+A P+FF+ P D +   + + FLLG  ++ +  L  +G+  +    P G W   F+++
Sbjct: 566 APVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILPRGIWHR-FDFA 625

Query: 742 ELVAVKSGQQITLDAPADHINVHVREGNILALHGEAM-TTQAARETAYKLLVAVSNGQSS 801
                        D+  D   ++++ G+I++L    +   + +      LLV++     +
Sbjct: 626 -------------DSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKA 678

Query: 802 FGEVFLDDGEVVEMGAEGGNWSLVRFYSEVVGSKLLVKSQVING 838
            G +F DDG+    G   G + +  + +E   S + VK     G
Sbjct: 686 KGLLFEDDGD--GYGYTKGRFLVTHYIAERDSSTVTVKVSKTEG 678