HG10021481 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10021481
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase
LocationChr05: 9806682 .. 9807835 (+)
RNA-Seq ExpressionHG10021481
SyntenyHG10021481
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCAATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGTTCAACACTTCAGAACAGGTTTGGTGTGTTTAAACATTCAGATTGGATTGGAAAACCATTTGGGTCCAAGGTGTTAAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAATAGTTCCTGGCTGTTTAATTCTAGAATCAGGGACGGGCAGTGGATCGTTGACTACGTCGTTAGCTAGGGCTGTAGCGCCTACTGGACATGTCTATACGTTTGATTTTCACGAACAGAGAGCTTCCTCAGCGAGGTAGGAACAAGGATCTATTGTATGTTCTCAAGTTACCCTGCAGAGTAATTGATATGTGATGGTTTTATTGTTAGATCTAGAGGTAGTTTAATAAGTAGTACTTATTTTCTTGTCTCTCTCTTCTTTCAGAGAGGATTTTGAGAAGACGGGCTTGACTAGCCTTGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAACGTTCAGCAGAAACTCTTCAATCTAAGTTTACAGGTTGCTTTGAATGATCGTATATGCCATTTAACTTCATTCCTAGTGACAATGTTAATTTGTATTGCTCATCTTTTGTCATCTCTTTCGTGTAGATATAAGGACATTTGAGGTATTGCTTCGCACTTACGAAGTTAAGGAAGGTAAAACAGAGAGTAGTCAGGTTGATGGAGGTGGTTCTGTTGGGCTTCCTCCATGTAAGAGACGGCTGAGATCAAAGGAATCAGACGACATGAATGATGATTCTAGTACGACGGTCATGGTTCGGCCGTGTAGTGAGGCTAGAGGTCACACGGGTTATCTAACATTTGCAAGACTTGGGTGCCTCTCATAA

mRNA sequence

ATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCAATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGTTCAACACTTCAGAACAGGTTTGGTGTGTTTAAACATTCAGATTGGATTGGAAAACCATTTGGGTCCAAGGTGTTAAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAATAGTTCCTGGCTGTTTAATTCTAGAATCAGGGACGGGCAGTGGATCGTTGACTACGTCGTTAGCTAGGGCTGTAGCGCCTACTGGACATGTCTATACGTTTGATTTTCACGAACAGAGAGCTTCCTCAGCGAGAGAGGATTTTGAGAAGACGGGCTTGACTAGCCTTGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAACGTTCAGCAGAAACTCTTCAATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGCACTTACGAAGTTAAGGAAGGTAAAACAGAGAGTAGTCAGGTTGATGGAGGTGGTTCTGTTGGGCTTCCTCCATGTAAGAGACGGCTGAGATCAAAGGAATCAGACGACATGAATGATGATTCTAGTACGACGGTCATGGTTCGGCCGTGTAGTGAGGCTAGAGGTCACACGGGTTATCTAACATTTGCAAGACTTGGGTGCCTCTCATAA

Coding sequence (CDS)

ATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCAATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGTTCAACACTTCAGAACAGGTTTGGTGTGTTTAAACATTCAGATTGGATTGGAAAACCATTTGGGTCCAAGGTGTTAAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTACTAAGCCACAGGACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAATAGTTCCTGGCTGTTTAATTCTAGAATCAGGGACGGGCAGTGGATCGTTGACTACGTCGTTAGCTAGGGCTGTAGCGCCTACTGGACATGTCTATACGTTTGATTTTCACGAACAGAGAGCTTCCTCAGCGAGAGAGGATTTTGAGAAGACGGGCTTGACTAGCCTTGTCACTGTGGGAGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATTCCTTCAGCTGAGAGAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAACGTTCAGCAGAAACTCTTCAATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGCACTTACGAAGTTAAGGAAGGTAAAACAGAGAGTAGTCAGGTTGATGGAGGTGGTTCTGTTGGGCTTCCTCCATGTAAGAGACGGCTGAGATCAAAGGAATCAGACGACATGAATGATGATTCTAGTACGACGGTCATGGTTCGGCCGTGTAGTGAGGCTAGAGGTCACACGGGTTATCTAACATTTGCAAGACTTGGGTGCCTCTCATAA

Protein sequence

MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLLRTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGYLTFARLGCLS
Homology
BLAST of HG10021481 vs. NCBI nr
Match: XP_038894928.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894929.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894930.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894931.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894932.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894933.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894934.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894935.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894936.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida] >XP_038894937.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa hispida])

HSP 1 Score: 609.8 bits (1571), Expect = 1.3e-170
Identity = 300/310 (96.77%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKH+DWIGKPFG
Sbjct: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES+QVDGGGSV LPPCKRRLRSKESDDMNDDSST VMVRPCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300

Query: 301 LTFARLGCLS 311
           LTFARL CLS
Sbjct: 301 LTFARLRCLS 310

BLAST of HG10021481 vs. NCBI nr
Match: XP_008459104.1 (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucumis melo] >KAA0043330.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucumis melo var. makuwa] >TYK02660.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 605.5 bits (1560), Expect = 2.5e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 58  MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 117

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 118 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 177

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 178 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 237

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 238 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 297

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES++VDGGGSV LPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 298 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 357

Query: 301 LTFARLGCLS 311
           LTFARLGCLS
Sbjct: 358 LTFARLGCLS 367

BLAST of HG10021481 vs. NCBI nr
Match: XP_008459105.1 (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucumis melo] >XP_008459106.1 PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A isoform X2 [Cucumis melo])

HSP 1 Score: 605.5 bits (1560), Expect = 2.5e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 7   MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 66

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 67  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 126

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 127 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 186

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 187 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 246

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES++VDGGGSV LPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 247 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 306

Query: 301 LTFARLGCLS 311
           LTFARLGCLS
Sbjct: 307 LTFARLGCLS 316

BLAST of HG10021481 vs. NCBI nr
Match: KAE8653334.1 (hypothetical protein Csa_023207, partial [Cucumis sativus])

HSP 1 Score: 605.1 bits (1559), Expect = 3.3e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK ISFTRSIRDGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKHSDWIGKPFG
Sbjct: 66  MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 125

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 126 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 185

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 186 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 245

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETL+SKFTDIRTFEVLL
Sbjct: 246 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 305

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES+QVDGGGSVGLPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 306 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 365

Query: 301 LTFARLGCLS 311
           LTFARL CLS
Sbjct: 366 LTFARLRCLS 375

BLAST of HG10021481 vs. NCBI nr
Match: XP_004135565.1 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sativus] >XP_011659360.1 tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sativus])

HSP 1 Score: 605.1 bits (1559), Expect = 3.3e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK ISFTRSIRDGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKHSDWIGKPFG
Sbjct: 1   MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 61  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETL+SKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES+QVDGGGSVGLPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 241 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 300

Query: 301 LTFARLGCLS 311
           LTFARL CLS
Sbjct: 301 LTFARLRCLS 310

BLAST of HG10021481 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.0e-65
Identity = 138/298 (46.31%), Postives = 191/298 (64.09%), Query Frame = 0

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           +VAPTGH++T +FH+QRA  ARE+F++  L+  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLQSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        +V LP       + E+ +M  D+S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLET-NMGSDASPFRSGTPMKETVGHTGYLTFA 285

BLAST of HG10021481 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.9e-64
Identity = 137/298 (45.97%), Postives = 186/298 (62.42%), Query Frame = 0

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLQSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        +V LPP    L +        D+S      P  EA GHTGYLTFA
Sbjct: 244 -------------TVSLPP--PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

BLAST of HG10021481 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.3e-63
Identity = 136/302 (45.03%), Postives = 190/302 (62.91%), Query Frame = 0

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           ++APTGH++T +FH+QRA  ARE+F++  ++  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLQSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVDGGGSVGLPPCKRRLRSKESDDM----NDDSSTTVMVRPCSEARGHTGYLT 305
                        +V LP     L    +DD+      D++      P  E  GHTGYLT
Sbjct: 244 -------------TVSLP-----LPDLGADDLEGNVGSDATPFRSGTPMKETVGHTGYLT 285

BLAST of HG10021481 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-60
Identity = 136/298 (45.64%), Postives = 184/298 (61.74%), Query Frame = 0

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHS-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +HS D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG ++ ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAERMLK-QDGVLCSFSPCIEQVQRSAETLQS-KFTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNVR 243

Query: 250 EGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGYLTFA 305
                        +V LP     L ++   D     S T    P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLP--VPDLGARPGPDAAPFRSGT----PMKETVGHTGYLTFA 280

BLAST of HG10021481 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.4e-60
Identity = 130/300 (43.33%), Postives = 187/300 (62.33%), Query Frame = 0

Query: 16  IRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFGSKVLSNKG-GFVYLL 75
           I++GD V++Y   D M  + +   +   ++FG ++H + IGK +GSK+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 76  APTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSGSLTTSLARAVAPTG 135
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 136 HVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQG-QGFPEEFVGL-ADSVFLDLPQP 195
           H++TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 196 WLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL-QSKFTDIRTFEVLLRTY-----EVK 255
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT+     E +
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 256 EGKTESSQVDGGGSVGLPPCKRRLRSK-ESDDMNDDSSTTVMVRPCSEARGHTGYLTFAR 306
           E    +  +D   +          R K E   +       ++ +P +EARGHTGYLTFAR
Sbjct: 248 ELNLTNPYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEARGHTGYLTFAR 307

BLAST of HG10021481 vs. ExPASy TrEMBL
Match: A0A1S3C9I4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103498313 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 7   MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 66

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 67  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 126

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 127 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 186

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 187 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 246

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES++VDGGGSV LPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 247 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 306

Query: 301 LTFARLGCLS 311
           LTFARLGCLS
Sbjct: 307 LTFARLGCLS 316

BLAST of HG10021481 vs. ExPASy TrEMBL
Match: A0A5A7TJ63 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1734G00040 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 58  MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 117

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 118 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 177

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 178 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 237

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 238 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 297

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES++VDGGGSV LPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 298 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 357

Query: 301 LTFARLGCLS 311
           LTFARLGCLS
Sbjct: 358 LTFARLGCLS 367

BLAST of HG10021481 vs. ExPASy TrEMBL
Match: A0A1S3C8X5 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103498313 PE=4 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG
Sbjct: 58  MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 117

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 118 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 177

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 178 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 237

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 238 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 297

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES++VDGGGSV LPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 298 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 357

Query: 301 LTFARLGCLS 311
           LTFARLGCLS
Sbjct: 358 LTFARLGCLS 367

BLAST of HG10021481 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 297/310 (95.81%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTDELK ISFTRSIRDGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKHSDWIGKPFG
Sbjct: 396 MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 455

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCL+LESGTGSG
Sbjct: 456 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 515

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 516 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 575

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDG+LCSFSPCIEQVQRS ETL+SKFTDIRTFEVLL
Sbjct: 576 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 635

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEVKEG+TES+QVDGGGSVGLPPCKRRLRSKESDDM+++SSTTVMVRPCSEARGHTGY
Sbjct: 636 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 695

Query: 301 LTFARLGCLS 311
           LTFARL CLS
Sbjct: 696 LTFARLRCLS 705

BLAST of HG10021481 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.0e-165
Identity = 291/310 (93.87%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPTD+LKKISFTRSIRDGDLVIVYERHD+MKAVKVCE ST QNRFG+FKHSDWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETL+SKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVKEGKTESSQVDGGGSVGLPPCKRRLRSKESDDMNDDSSTTVMVRPCSEARGHTGY 300
           RTYEV+EGKTESSQV+GGGSV LPPCK+R+RSKE++D N DSS TVMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSS-TVMVRPCSEARGHTGY 300

Query: 301 LTFARLGCLS 311
           LTFARL CLS
Sbjct: 301 LTFARLRCLS 309

BLAST of HG10021481 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 483.4 bits (1243), Expect = 1.4e-136
Identity = 238/318 (74.84%), Postives = 271/318 (85.22%), Query Frame = 0

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 60
           MLPT+  K  SF R I DGDLVIVYERHD+MK VKV +   LQNRFGV+KHSDWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120
           +KV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LE+VPGC++LESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SL+TSLARAVAPTGHVY+FDFHEQRA SAREDFEKTG++SLVTV VRDIQG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLQSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLA+PSA +MLKQDGVLCSFSPCIEQVQR+ E L+S F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 241 RTYEVKEGKTESSQVDGGG----SVGLPPCKRRLRSKESDDMNDD----SSTTVMVRPCS 300
           RTYEVKE K ++S + G      + G+ PCKR+ RS E+D ++ D    +S+ VM RPCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 301 EARGHTGYLTFARLGCLS 311
           EARGHTGYLTFARL CL+
Sbjct: 301 EARGHTGYLTFARLQCLA 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894928.11.3e-17096.77tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Benincasa h... [more]
XP_008459104.12.5e-16995.81PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A i... [more]
XP_008459105.12.5e-16995.81PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A i... [more]
KAE8653334.13.3e-16995.81hypothetical protein Csa_023207, partial [Cucumis sativus][more]
XP_004135565.13.3e-16995.81tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A [Cucumis sat... [more]
Match NameE-valueIdentityDescription
Q80XC26.0e-6546.31tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
Q96FX73.9e-6445.97tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
Q6AY463.3e-6345.03tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
A6H7911.2e-6045.64tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
Q86JJ03.4e-6043.33tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
Match NameE-valueIdentityDescription
A0A1S3C9I41.2e-16995.81tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC10349831... [more]
A0A5A7TJ631.2e-16995.81tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C8X51.2e-16995.81tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC10349831... [more]
A0A0A0LYQ81.6e-16995.81(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
A0A6J1H9F47.0e-16593.87tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT5G14600.11.4e-13674.84S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 69..305
e-value: 8.8E-97
score: 323.6
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PIRSFPIRSF017269GCD14coord: 10..308
e-value: 2.8E-98
score: 326.2
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PANTHERPTHR12133TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASEcoord: 2..306
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROSITEPS51620SAM_TRM61coord: 19..307
score: 97.166626
NoneNo IPR availableGENE3D3.10.330.20coord: 10..77
e-value: 2.5E-26
score: 93.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..307
e-value: 1.7E-79
score: 268.4
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 47..251
e-value: 2.1E-41
score: 139.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..285
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 2..306
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 15..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10021481.1HG10021481.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity