HG10021460 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021460 vs. NCBI nr
Match: XP_038895846.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.4e-292
Identity = 486/550 (88.36%), Postives = 518/550 (94.18%), Query Frame = 0

Query: 1   MECFPLTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPT 60
           MECFPLTAP G IAF II IVL+TTTTSFCTQES+SFVQCLF YGS NT+PIS V+YTP 
Sbjct: 1   MECFPLTAPLGRIAF-IIFIVLTTTTTSFCTQESDSFVQCLFDYGSLNTHPISDVIYTPI 60

Query: 61  NSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSS+LNFS+QNLRFLTK+TPKPLAIITPSHVSHIQAAVICSKSHG QIRTRSGGHDF
Sbjct: 61  NSSYSSVLNFSVQNLRFLTKKTPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDF 120

Query: 121 EGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSC 180
           EGLSYISD PF+VVDLINLRSI IDVENENAWVQSGATLGELYYRIA+KSQTLAFPAGSC
Sbjct: 121 EGLSYISDAPFIVVDLINLRSIAIDVENENAWVQSGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGG 240
            TVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGK+HDRESMGEDLFWAIRGGGGG
Sbjct: 181 STVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKLHDRESMGEDLFWAIRGGGGG 240

Query: 241 SFGIIVDWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGK 300
           SFGI+V WK+KLVRVPATVTVCEANRTLEED MKLI+EWQYVAPKLDANLLLGISLTGGK
Sbjct: 241 SFGIVVSWKVKLVRVPATVTVCEANRTLEEDVMKLIHEWQYVAPKLDANLLLGISLTGGK 300

Query: 301 VTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQ 360
           VTED+EGKINPIASF A FLGKAEELM ILKPTFSQLGLK+EDCLE SWIESVL+MGWFQ
Sbjct: 301 VTEDEEGKINPIASFVAQFLGKAEELMTILKPTFSQLGLKREDCLEISWIESVLLMGWFQ 360

Query: 361 KEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGK 420
           KEDP EVLLNRTRLYSEISKIKSDYVKERIP +S+KGMWERLKSQDVEL+QIMFVPYGG+
Sbjct: 361 KEDPIEVLLNRTRLYSEISKIKSDYVKERIPKASIKGMWERLKSQDVELAQIMFVPYGGR 420

Query: 421 MSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAA 480
           MSEVY+WETPYSHR DYMYLIGYI KWK G VEAEKRHLNWIREIYDYMTPFVS SPR A
Sbjct: 421 MSEVYEWETPYSHRGDYMYLIGYIAKWKNGTVEAEKRHLNWIREIYDYMTPFVSNSPRVA 480

Query: 481 YINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 540
           Y+NYRDLD+GTNNKY KTSY+RAR WG+KYFGNNFDRLVY+KTKVDPS+FF+HEQSIP L
Sbjct: 481 YVNYRDLDIGTNNKYDKTSYKRARNWGMKYFGNNFDRLVYIKTKVDPSNFFKHEQSIPIL 540

Query: 541 ASVSEELFNA 551
             ++EE F+A
Sbjct: 541 TGITEEFFSA 549

BLAST of HG10021460 vs. NCBI nr
Match: XP_008445757.1 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo] >KAA0047085.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa] >TYK05070.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa])

HSP 1 Score: 974.5 bits (2518), Expect = 3.7e-280
Identity = 473/554 (85.38%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MECFP-LTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTP 60
           M+CFP L APFG IAF II++VLST TTSFCTQE +SFVQCLF YGSPN+NPI++V+YTP
Sbjct: 1   MKCFPILAAPFGLIAF-IIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTP 60

Query: 61  TNSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120
           +NSSYSSILNFSIQNLRFL K   KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD
Sbjct: 61  SNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 FEGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGS 180
           FEGLSYIS VPFV+VDLINL SI+IDVEN+NAWV+SGATLGELYYRIA+KSQTLAFPAGS
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGS 180

Query: 181 CPTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVH++ESMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGG 240

Query: 241 GSFGIIVDWKLKLVRVPATVTVCEANRTLEED-TMKLINEWQYVAPKLDANLLLGISLTG 300
           G FGI+V WK+KLV VPA VT+CEANRTLEED  +KLI+EWQYVAPKLDANLLLGISLTG
Sbjct: 241 GCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTG 300

Query: 301 GKVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGW 360
           GKVTED+EGKINPIASFS MFLGKAEE++ ILKPTF QL L KEDCLE SWI+SVL+MGW
Sbjct: 301 GKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGW 360

Query: 361 FQKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYG 420
           FQK DP EVLLNRTRLYSEISKIKSDYVKE IP  +LKGMWERLKS+DVELSQIMFVPYG
Sbjct: 361 FQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYG 420

Query: 421 GKMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPR 480
           GKMSEV D ETP+SHR+DY YL+GYI KWK G VEAE+RHLNWIREIYDYMTPFVSKSPR
Sbjct: 421 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPR 480

Query: 481 AAYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 540
           AAY+NYRDLD+GTNNKYG+TSY+RARVWGLKYFG NFD+LVYVKTKVDPSDFFRH+QSIP
Sbjct: 481 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIP 540

Query: 541 TLASVSEELFNASL 553
           TL+ +SEE F+ASL
Sbjct: 541 TLSGISEESFSASL 553

BLAST of HG10021460 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 474/556 (85.25%), Postives = 517/556 (92.99%), Query Frame = 0

Query: 1   MECFP-LTAPFGSIAFTIII-IVLSTTTTSFC-TQESNSFVQCLFHYGSPNTNPISSVVY 60
           M+CFP L APFG IAF III +VLST T SFC TQES SFVQCLF YGS +TNPI++++Y
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPTNSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120
           TPTNSSYSSILNFSIQNLRFL K+ PKP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPA 180
           HDFEGLSYISD+PFV+VDLINL+SI+IDVEN+NAWV SGATLGELYYRI++KSQTLAFPA
Sbjct: 121 HDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVH++ESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGSFGIIVDWKLKLVRVPATVTVCEANRTLEED-TMKLINEWQYVAPKLDANLLLGISL 300
           GGGSFGI+V WK+KLV VPA VT+CEANRTLEED  +KLI+EWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMM 360
           TGGKVTED+EGKINPIASFS MFLGKAEE++ ILKPTF QLGL KEDCLE SWI+SVL+M
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVP 420
           GWFQKEDP EVLLNR+RLYSEISKIKSDYVKE IP  ++KGMWERLKSQDVELSQIMFVP
Sbjct: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420

Query: 421 YGGKMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKS 480
           YGGKMSEV D ETP+SHR+DY YL+GYI KWK G VEAEK HLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQS 540
           PRAAY+NYRDLD+GTNNKYG+TSY+RARVWGLKYFG NFDRLV+VKTKVDPSDFFRHEQS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540

Query: 541 IPTLASVSEELFNASL 553
           IPTL+ +S+ELF+ASL
Sbjct: 541 IPTLSGISKELFSASL 556

BLAST of HG10021460 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 784.6 bits (2025), Expect = 5.4e-223
Identity = 390/544 (71.69%), Postives = 455/544 (83.64%), Query Frame = 0

Query: 1   MECFPLTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPT 60
           M+ F LT PF   A T I++ +S +   F T++  +FVQCL +  S +T PIS+++YTP 
Sbjct: 1   MKSFLLT-PF---ALTFIVLSISPSWVVFSTKKHENFVQCLLN-NSLSTYPISNLIYTPI 60

Query: 61  NSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSSILNFSIQNLRF +KETPKPLAIITPSHVSHIQAA+ICSKSH  QIRTRSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRSGGHDF 120

Query: 121 EGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSC 180
           EGLSYISDVPFVVVDLINL+SI IDVENE AWVQSGATLGELYYRIA+KS+TLAFPAGSC
Sbjct: 121 EGLSYISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGG+GWL+RKYG+AAD+VIDA  +DANGKV+DRESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGG 240

Query: 241 SFGIIVDWKLKLVRVPATVTVCEANRTL-EEDTMKLINEWQYVAPKLDANLLLGISLTGG 300
           SFGI+V WK+KLVRVPATVT+C  NR L EEDT+KLI+ WQYV  KLD NLLLGISL GG
Sbjct: 241 SFGIVVAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGISLIGG 300

Query: 301 KVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWF 360
             T+ + GK NP+A FS+ FLGK  EL+ IL   FS+LGL KEDC E SWI+SVL+M  F
Sbjct: 301 NSTQ-EGGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVLIMAGF 360

Query: 361 QKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGG 420
            K++  EVLLNR       +KIKSDYVKE I  +S K M++RLK++D+E++QIMF+PYGG
Sbjct: 361 PKQEALEVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMFIPYGG 420

Query: 421 KMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRA 480
           +MSE+ +  TP+ HRA  +Y +GY VKWKE  ++AEKRHLNW+R IYDYMTPFVSKSPRA
Sbjct: 421 RMSEISESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVSKSPRA 480

Query: 481 AYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPT 540
           AY NYRDLD+GTNNK GKTSY RARVWGLKYFG NFDRLV+VKTK+DP++FFR+EQSIPT
Sbjct: 481 AYNNYRDLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNEQSIPT 538

Query: 541 LASV 544
           L  +
Sbjct: 541 LTDI 538

BLAST of HG10021460 vs. NCBI nr
Match: XP_022152433.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 761.5 bits (1965), Expect = 4.9e-216
Identity = 369/542 (68.08%), Postives = 438/542 (80.81%), Query Frame = 0

Query: 1   MECFPLTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPT 60
           M+ FPL  P  + AF   I++ S+   +    +   F+QCL  + SP+ N IS  +YTP 
Sbjct: 1   MKSFPLN-PL-AFAFACSIVLSSSLFWAASAHKHEGFLQCLTRH-SPSNNSISKFIYTPA 60

Query: 61  NSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           N SYSSILNFSIQNLRF T ETPKPL +I PSH+SHIQAAVICSK+HG QIRTRSGGHDF
Sbjct: 61  NFSYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDF 120

Query: 121 EGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSC 180
           EGLSY+S +PF+V+DLI+LRSITID +N  AWVQSGATLGELYYRIA+KSQTLAFPAGSC
Sbjct: 121 EGLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGGYG+LIRKYG+AADHVIDA+LVDANG++ +RESM +DLFW IRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGG 240

Query: 241 SFGIIVDWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGK 300
           SFGI+V WKL+LV VP T+T C  NRTL+ D +KLI+ WQYVA KL+ NLLLGISLTGG 
Sbjct: 241 SFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGH 300

Query: 301 VTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQ 360
           +   Q GK NP+ASF   FLGK +EL+AI+  TF +LGL KEDC+ETSWIES L+   F 
Sbjct: 301 ILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFS 360

Query: 361 KEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGK 420
             +  E LL R  L +  +KIKSDYVKE I  +  KG+WERL +QD+EL+Q+MFVPYGG+
Sbjct: 361 AGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGR 420

Query: 421 MSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAA 480
           MSE  + ETPY HRA+ MY +GYIVKW+E   +AEKRHLNW+REIYDYM P+V+KSPRAA
Sbjct: 421 MSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAA 480

Query: 481 YINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 540
           Y+NYRDLD+G NN+Y KT+Y RA+VWGLKYFG NF+RLV VKT+VDPSDFFRHEQSIPT 
Sbjct: 481 YVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTF 539

Query: 541 AS 543
           +S
Sbjct: 541 SS 539

BLAST of HG10021460 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 3.0e-147
Identity = 260/539 (48.24%), Postives = 366/539 (67.90%), Query Frame = 0

Query: 15  FTIIIIVLSTTT--------TSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSS 74
           F+ ++I  +T+T         S      +SF+QCL      ++N +S V++TP ++S+SS
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSS 66

Query: 75  ILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYI 134
           +L  SIQN RF   + PKP+ I+TP   S +Q+AV C++  G  IRTRSGGHD+EGLSY+
Sbjct: 67  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 126

Query: 135 SDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVG 194
           +  PFV++DL NLRSIT+DV+N + WVQ+GAT+GELYY I KK++TLAFPAG CPTVGVG
Sbjct: 127 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 186

Query: 195 GHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIV 254
           GH SGGGYG L+RK+G+AADHVIDA +VDA G++ +R  MGED FWAIRGGGG SF +++
Sbjct: 187 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 246

Query: 255 DWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQE 314
            WK+ L+ VP+TVTV    +  E+  +K+I+ WQ+VA K+  +L + + L        Q 
Sbjct: 247 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--------QR 306

Query: 315 GKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQK--EDP 374
            K    ASF  ++LG  + L+ ++   F +LGL+++DC E SWIESV+   WF +  E+P
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEP 366

Query: 375 FEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEV 434
             VL  RTR  S   K KSD+V+E +P +++  +W RL+  + E +Q++F P+GGKMSE+
Sbjct: 367 INVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 426

Query: 435 YDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINY 494
            D+ETP+ HR   +Y I Y+  W+    + +++++ W+  +YD M+ FV+KSPR AYIN 
Sbjct: 427 ADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 486

Query: 495 RDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTLASV 544
           RDLD+G      ++ Y   + WG+KYF NNF+RLV VKT VDPSDFF  EQSIP    V
Sbjct: 487 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFTFV 529

BLAST of HG10021460 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 3.1e-144
Identity = 260/530 (49.06%), Postives = 354/530 (66.79%), Query Frame = 0

Query: 17  IIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSSILNFSIQNLR 76
           II    S    +       +F++C   Y  PN      +VYT  N  Y S+LN +I NLR
Sbjct: 14  IIFFFFSFNIQTSIANPRENFLKCFSQY-IPNNATNLKLVYTQNNPLYMSVLNSTIHNLR 73

Query: 77  FLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDVPFVVVDL 136
           F +  TPKPL I+TPSHVSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS VPFV+VDL
Sbjct: 74  FTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDL 133

Query: 137 INLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSGGGYGW 196
            N+RSI IDV ++ AWV++GATLGE+YY + +K++ L+  AG CPTV  GGH  GGGYG 
Sbjct: 134 RNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGP 193

Query: 197 LIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLKLVRVP 256
           L+R YG+AAD++IDA+LV+ +GKV DR+SMGEDLFWA+RGGG  SFGIIV WK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVP 253

Query: 257 ATVTVCEANRTLE-EDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQ-EGKINPIAS 316
            + T+    + +E  + +KL+N+WQ +A K D +LLL        +T++Q + K      
Sbjct: 254 KS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTY 313

Query: 317 FSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQKEDP----FEVLLN 376
           FS++FLG  + L+ ++  +F +LG+KK DC + SWI++++        D      E+LL+
Sbjct: 314 FSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLD 373

Query: 377 RTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDWETP 436
           R+   +   KIK DYVK+ IP S    + E+L  +D+        PYGG M E+ +   P
Sbjct: 374 RSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 433

Query: 437 YSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINYRDLDVG 496
           + HRA  +Y + YI  W++   E  ++HLNWIR IY++MTP+VSK+PR AY+NYRDLD+G
Sbjct: 434 FPHRAGILYELWYICSWEK--QEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 493

Query: 497 TNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 541
            N+     +Y +AR+WG KYFG NFDRLV VKT VDP++FFR+EQSIP L
Sbjct: 494 INDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of HG10021460 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 5.2e-144
Identity = 258/534 (48.31%), Postives = 358/534 (67.04%), Query Frame = 0

Query: 17  IIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSSILNFSIQNLR 76
           II   LS            +F++C   Y  P     + +VYT  +  Y SILN +IQNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCFSQY-IPTNVTNAKLVYTQHDQFYMSILNSTIQNLR 73

Query: 77  FLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDVPFVVVDL 136
           F +  TPKPL IITP +VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS VPFV+VDL
Sbjct: 74  FTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 137 INLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSGGGYGW 196
            N+ S+ IDV ++ AWV++GATLGE+YY I + ++ L+FPAG CPTVG GGH SGGGYG 
Sbjct: 134 RNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGA 193

Query: 197 LIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLKLVRVP 256
           L+R YG+AAD++IDA+LV+ +GKV DR+SMGEDLFWAIRGGGG +FGII  WK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 257 ATVTVCEANRTLE-EDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQ-EGKINPIAS 316
           +  T+    + +E  + +KL+N+WQ +A   +  LLL        +T++Q + K    + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 317 FSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMG--------WFQKEDPFE 376
           FS++F G  + L+ ++  +F +LG+KK DC + SWI++++           +F+K    E
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKK----E 373

Query: 377 VLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYD 436
           +LL+R+        IK DYVK+ IP +++  + E+L  +DV +   +F PYGG M E+ +
Sbjct: 374 ILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISE 433

Query: 437 WETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINYRD 496
              P+ HRA  MY I YI  W++   E  ++H+NWIR +Y++ TP+VS++PR AY+NYRD
Sbjct: 434 SAIPFPHRAGIMYEIWYIASWEK--QEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRD 493

Query: 497 LDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 541
           LD+G  N     +Y +AR+WG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 494 LDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of HG10021460 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 257/525 (48.95%), Postives = 359/525 (68.38%), Query Frame = 0

Query: 28  SFCTQESN--SFVQCLFHYGSPNTNPISSVVYTPTNSSYSSILNFSIQNLRFLTKETPKP 87
           S   Q +N  +F++CL H  + +    S +++T  + SY SILN SIQN RF   ETPKP
Sbjct: 26  SISAQRTNHENFLKCLSHRINEDD---SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKP 85

Query: 88  LAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS-DVPFVVVDLINLRSITI 147
           ++IITP   + +Q+ + C++ HG  IRTRSGGHD+EGLSY++   PFVV+DL NLRSIT+
Sbjct: 86  VSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITL 145

Query: 148 DVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGIA 207
           DV+N   WVQSGAT+GELYY I K S++LAFPAG  PTVG+GG   GGGYG L+RKYG++
Sbjct: 146 DVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLS 205

Query: 208 ADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLKLVRVPATVTVCEA 267
           AD+VIDA++VDANG   DR+ MGED FWAIRGGGG SF +++ WK++L+ VP+ VTV + 
Sbjct: 206 ADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKV 265

Query: 268 NRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQEGKINPIASFSAMFLGKAE 327
            +T E++ + +IN+WQY+A K+  +L +   L   K TE         ASF  ++LG   
Sbjct: 266 VKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--KETE-------VYASFPGLYLGPVS 325

Query: 328 ELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQKEDPFEVLLNRTRLYSEISKIKSD 387
           +L+A++K  F +LGL+  +C E SWIESVL   WF K +  E+L  R R  S   K K D
Sbjct: 326 DLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRKRT-SRSFKGKDD 385

Query: 388 YVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDWETPYSHRADYMYLIGYI 447
           +++E IP ++++ +W R ++ +  L++I+  P+GGKMSE+ D E P+ HR   +Y I Y+
Sbjct: 386 FIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYL 445

Query: 448 VKWKEGGVEAE---KRHLNWIREIYDYMTPFVSKSPRAAYINYRDLDVGTNNKYG-KTSY 507
             W E   + +   +++L W+  +Y++MTP+VSKSPR AY+N+RD+D+G       KT Y
Sbjct: 446 AYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKY 505

Query: 508 RRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTLASVSE 546
             A+VWG+KYF NNFDRLV VKT VDP DFF  EQSIP +  V++
Sbjct: 506 EEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIMKYVND 534

BLAST of HG10021460 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.6e-143
Identity = 255/530 (48.11%), Postives = 356/530 (67.17%), Query Frame = 0

Query: 17  IIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSSILNFSIQNLR 76
           II   LS            +F++CL  Y  P     + +VYT  +  Y SILN ++QNLR
Sbjct: 14  IIFFFLSFNIQISIANPQENFLKCLSQY-IPTNVTNAKLVYTQHDQFYMSILNSTVQNLR 73

Query: 77  FLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDVPFVVVDL 136
           F +  TPKPL I TP +VSHIQ  ++CSK  G QIRTRSGGHD EG+SYIS VPFV+VDL
Sbjct: 74  FTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDL 133

Query: 137 INLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSGGGYGW 196
            N+ S+ IDV ++ AWV+SGATLGE+YY I + ++ L+FPAG CPTVG GGH SGGGYG 
Sbjct: 134 RNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGA 193

Query: 197 LIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLKLVRVP 256
           L+R YG+AAD++IDA+LV+ +GKV DR+SMGEDLFWAIRGGGG +FGII  WK++LV VP
Sbjct: 194 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVP 253

Query: 257 ATVTVCEANRTLE-EDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQ-EGKINPIAS 316
           +  T+    + +E  + +KL+N+WQ +A   +  LLL        +T++Q + K    + 
Sbjct: 254 SMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSY 313

Query: 317 FSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVL----MMGWFQKEDPFEVLLN 376
           FS++F G  + L+ ++  +F +LG+KK DC + SWI++++    ++ +       E+LL+
Sbjct: 314 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLD 373

Query: 377 RTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDWETP 436
           R+        IK DYVK+ IP +++  + E+L  +DV +   +F PYGG M E+ +   P
Sbjct: 374 RSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIP 433

Query: 437 YSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINYRDLDVG 496
           + HRA   Y I YI  W++   E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G
Sbjct: 434 FPHRAGITYEIWYIASWEK--QEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLG 493

Query: 497 TNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 541
             N     +Y +AR+WG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 494 KTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of HG10021460 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.8e-280
Identity = 473/554 (85.38%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MECFP-LTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTP 60
           M+CFP L APFG IAF II++VLST TTSFCTQE +SFVQCLF YGSPN+NPI++V+YTP
Sbjct: 1   MKCFPILAAPFGLIAF-IIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTP 60

Query: 61  TNSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120
           +NSSYSSILNFSIQNLRFL K   KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD
Sbjct: 61  SNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 FEGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGS 180
           FEGLSYIS VPFV+VDLINL SI+IDVEN+NAWV+SGATLGELYYRIA+KSQTLAFPAGS
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGS 180

Query: 181 CPTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVH++ESMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGG 240

Query: 241 GSFGIIVDWKLKLVRVPATVTVCEANRTLEED-TMKLINEWQYVAPKLDANLLLGISLTG 300
           G FGI+V WK+KLV VPA VT+CEANRTLEED  +KLI+EWQYVAPKLDANLLLGISLTG
Sbjct: 241 GCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTG 300

Query: 301 GKVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGW 360
           GKVTED+EGKINPIASFS MFLGKAEE++ ILKPTF QL L KEDCLE SWI+SVL+MGW
Sbjct: 301 GKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGW 360

Query: 361 FQKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYG 420
           FQK DP EVLLNRTRLYSEISKIKSDYVKE IP  +LKGMWERLKS+DVELSQIMFVPYG
Sbjct: 361 FQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYG 420

Query: 421 GKMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPR 480
           GKMSEV D ETP+SHR+DY YL+GYI KWK G VEAE+RHLNWIREIYDYMTPFVSKSPR
Sbjct: 421 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPR 480

Query: 481 AAYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 540
           AAY+NYRDLD+GTNNKYG+TSY+RARVWGLKYFG NFD+LVYVKTKVDPSDFFRH+QSIP
Sbjct: 481 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIP 540

Query: 541 TLASVSEELFNASL 553
           TL+ +SEE F+ASL
Sbjct: 541 TLSGISEESFSASL 553

BLAST of HG10021460 vs. ExPASy TrEMBL
Match: A0A1S3BEA9 (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.8e-280
Identity = 473/554 (85.38%), Postives = 515/554 (92.96%), Query Frame = 0

Query: 1   MECFP-LTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTP 60
           M+CFP L APFG IAF II++VLST TTSFCTQE +SFVQCLF YGSPN+NPI++V+YTP
Sbjct: 1   MKCFPILAAPFGLIAF-IIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTP 60

Query: 61  TNSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120
           +NSSYSSILNFSIQNLRFL K   KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD
Sbjct: 61  SNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 FEGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGS 180
           FEGLSYIS VPFV+VDLINL SI+IDVEN+NAWV+SGATLGELYYRIA+KSQTLAFPAGS
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGS 180

Query: 181 CPTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVH++ESMGEDLFWAIRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGG 240

Query: 241 GSFGIIVDWKLKLVRVPATVTVCEANRTLEED-TMKLINEWQYVAPKLDANLLLGISLTG 300
           G FGI+V WK+KLV VPA VT+CEANRTLEED  +KLI+EWQYVAPKLDANLLLGISLTG
Sbjct: 241 GCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTG 300

Query: 301 GKVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGW 360
           GKVTED+EGKINPIASFS MFLGKAEE++ ILKPTF QL L KEDCLE SWI+SVL+MGW
Sbjct: 301 GKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGW 360

Query: 361 FQKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYG 420
           FQK DP EVLLNRTRLYSEISKIKSDYVKE IP  +LKGMWERLKS+DVELSQIMFVPYG
Sbjct: 361 FQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYG 420

Query: 421 GKMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPR 480
           GKMSEV D ETP+SHR+DY YL+GYI KWK G VEAE+RHLNWIREIYDYMTPFVSKSPR
Sbjct: 421 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPR 480

Query: 481 AAYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 540
           AAY+NYRDLD+GTNNKYG+TSY+RARVWGLKYFG NFD+LVYVKTKVDPSDFFRH+QSIP
Sbjct: 481 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIP 540

Query: 541 TLASVSEELFNASL 553
           TL+ +SEE F+ASL
Sbjct: 541 TLSGISEESFSASL 553

BLAST of HG10021460 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.4e-216
Identity = 369/542 (68.08%), Postives = 438/542 (80.81%), Query Frame = 0

Query: 1   MECFPLTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPT 60
           M+ FPL  P  + AF   I++ S+   +    +   F+QCL  + SP+ N IS  +YTP 
Sbjct: 1   MKSFPLN-PL-AFAFACSIVLSSSLFWAASAHKHEGFLQCLTRH-SPSNNSISKFIYTPA 60

Query: 61  NSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           N SYSSILNFSIQNLRF T ETPKPL +I PSH+SHIQAAVICSK+HG QIRTRSGGHDF
Sbjct: 61  NFSYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDF 120

Query: 121 EGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSC 180
           EGLSY+S +PF+V+DLI+LRSITID +N  AWVQSGATLGELYYRIA+KSQTLAFPAGSC
Sbjct: 121 EGLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGGYG+LIRKYG+AADHVIDA+LVDANG++ +RESM +DLFW IRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGG 240

Query: 241 SFGIIVDWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGK 300
           SFGI+V WKL+LV VP T+T C  NRTL+ D +KLI+ WQYVA KL+ NLLLGISLTGG 
Sbjct: 241 SFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGVKLIHRWQYVAHKLEDNLLLGISLTGGH 300

Query: 301 VTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQ 360
           +   Q GK NP+ASF   FLGK +EL+AI+  TF +LGL KEDC+ETSWIES L+   F 
Sbjct: 301 ILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFS 360

Query: 361 KEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGK 420
             +  E LL R  L +  +KIKSDYVKE I  +  KG+WERL +QD+EL+Q+MFVPYGG+
Sbjct: 361 AGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGR 420

Query: 421 MSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAA 480
           MSE  + ETPY HRA+ MY +GYIVKW+E   +AEKRHLNW+REIYDYM P+V+KSPRAA
Sbjct: 421 MSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAA 480

Query: 481 YINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 540
           Y+NYRDLD+G NN+Y KT+Y RA+VWGLKYFG NF+RLV VKT+VDPSDFFRHEQSIPT 
Sbjct: 481 YVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTF 539

Query: 541 AS 543
           +S
Sbjct: 541 SS 539

BLAST of HG10021460 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 5.0e-214
Identity = 364/544 (66.91%), Postives = 442/544 (81.25%), Query Frame = 0

Query: 1   MECFPLTAPFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPT 60
           M+ F LT PF   A   I++   + + +  T++  +F+QCLF+        IS+++YTP 
Sbjct: 3   MKSFLLT-PF---ALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPI 62

Query: 61  NSSYSSILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSS+ S+LNFSIQNLRF  K+TPKPLAIITPSHVSHIQAA+ICSKSH  QIR RSGGHDF
Sbjct: 63  NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDF 122

Query: 121 EGLSYISDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSC 180
           EGLSY+SDVPF+VVDLINLRSITIDVE ENAWVQSGATLGE YYRI +KS+TLAFPAGSC
Sbjct: 123 EGLSYVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSC 182

Query: 181 PTVGVGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGG 240
           PTVG+GGHLSGGG+GWL+RKYG+AAD+VIDA  +DANGKV+DRESMGEDLFWAIRGGGGG
Sbjct: 183 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGG 242

Query: 241 SFGIIVDWKLKLVRVPATVTVCEANRTL-EEDTMKLINEWQYVAPKLDANLLLGISLTGG 300
           SFGIIV WK+KLVRVPATVT+C + R+L EEDT+KLI++WQY+   LD NLLLGISLTGG
Sbjct: 243 SFGIIVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGG 302

Query: 301 KVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWF 360
            +T+ + GK NP A FS+ FLGK  ELM IL   F +L L KE+C E SWI++VL+M  F
Sbjct: 303 NLTQ-ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGF 362

Query: 361 QKEDPFEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGG 420
              +PFEVLLNRT L+   +KIKSDY+K+ +  ++ K M +RLK++D+E++QIMF+PYGG
Sbjct: 363 PTHEPFEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGG 422

Query: 421 KMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRA 480
           +MSE+   ETP+ HR   +Y +GY VKWK+  ++AEKRHL+WIR+IYDYMTPFVSKSPRA
Sbjct: 423 RMSEISKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRA 482

Query: 481 AYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPT 540
            Y NYRDLD+G NNKYGK +Y  ARVWG KYFG NFDRLV+VKT +DP+DFFR+EQSIP 
Sbjct: 483 TYSNYRDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPA 541

Query: 541 LASV 544
           L  +
Sbjct: 543 LIDI 541

BLAST of HG10021460 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 4.2e-213
Identity = 361/535 (67.48%), Postives = 440/535 (82.24%), Query Frame = 0

Query: 8   APFGSIAFTIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTN-PISSVVYTPTNSSYSS 67
           +P  S++   I +  S+   S    +  SF+QCL  +    +N  IS V++TP NSSYSS
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 68  ILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYI 127
           +L+FSI+NLRF T ETPKPL I+TPSHVSHIQAAVICSKS+GFQIRTRSGGHDFEGLSY+
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 128 SDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVG 187
           + +PF++VDLINLRSI+ID+++  AWVQSGATLGELYY IA+KS+TLAFPAG CPTVGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 188 GHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIV 247
           GH SGGGYG L+RKYG+AAD+VIDAYLVDA+GK H+RESMGEDLFWAIRGGGGGSFGI+V
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 248 DWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQE 307
            WK+KLV VPATVT+C ANRTLEE  +K+ + WQYVA KLD NL LGI   GGK+T  + 
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 308 GKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMG-WFQKEDPF 367
           GK NP+A+F ++FLGKA+EL++IL   F +L L K+DC+ETSWIES ++MG  F  E   
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTL 364

Query: 368 EVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVY 427
           E LL+RT L +  SKIKSDYVKE +  + ++G+W+RL SQD+E   I+FVPYGG+MS++ 
Sbjct: 365 EALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQIS 424

Query: 428 DWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINYR 487
           + ETP+SHRA Y+Y IGYI  W+E G++AEKRHL WIRE+YDYMTPFVSKSPRAAY NYR
Sbjct: 425 ESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYR 484

Query: 488 DLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTL 541
           DLD+G+NNK+GKTSY++A VWGLKYFGNNF+RLV+VKTKVDP DFFRHEQSIP L
Sbjct: 485 DLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of HG10021460 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.1e-148
Identity = 260/539 (48.24%), Postives = 366/539 (67.90%), Query Frame = 0

Query: 15  FTIIIIVLSTTT--------TSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSS 74
           F+ ++I  +T+T         S      +SF+QCL      ++N +S V++TP ++S+SS
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL-SLQLNDSNIVSKVIHTPNDTSFSS 66

Query: 75  ILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYI 134
           +L  SIQN RF   + PKP+ I+TP   S +Q+AV C++  G  IRTRSGGHD+EGLSY+
Sbjct: 67  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 126

Query: 135 SDVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVG 194
           +  PFV++DL NLRSIT+DV+N + WVQ+GAT+GELYY I KK++TLAFPAG CPTVGVG
Sbjct: 127 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 186

Query: 195 GHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIV 254
           GH SGGGYG L+RK+G+AADHVIDA +VDA G++ +R  MGED FWAIRGGGG SF +++
Sbjct: 187 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 246

Query: 255 DWKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQE 314
            WK+ L+ VP+TVTV    +  E+  +K+I+ WQ+VA K+  +L + + L        Q 
Sbjct: 247 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--------QR 306

Query: 315 GKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQK--EDP 374
            K    ASF  ++LG  + L+ ++   F +LGL+++DC E SWIESV+   WF +  E+P
Sbjct: 307 YKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---WFAELGEEP 366

Query: 375 FEVLLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEV 434
             VL  RTR  S   K KSD+V+E +P +++  +W RL+  + E +Q++F P+GGKMSE+
Sbjct: 367 INVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 426

Query: 435 YDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINY 494
            D+ETP+ HR   +Y I Y+  W+    + +++++ W+  +YD M+ FV+KSPR AYIN 
Sbjct: 427 ADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 486

Query: 495 RDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTLASV 544
           RDLD+G      ++ Y   + WG+KYF NNF+RLV VKT VDPSDFF  EQSIP    V
Sbjct: 487 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFTFV 529

BLAST of HG10021460 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 257/525 (48.95%), Postives = 359/525 (68.38%), Query Frame = 0

Query: 28  SFCTQESN--SFVQCLFHYGSPNTNPISSVVYTPTNSSYSSILNFSIQNLRFLTKETPKP 87
           S   Q +N  +F++CL H  + +    S +++T  + SY SILN SIQN RF   ETPKP
Sbjct: 26  SISAQRTNHENFLKCLSHRINEDD---SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKP 85

Query: 88  LAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS-DVPFVVVDLINLRSITI 147
           ++IITP   + +Q+ + C++ HG  IRTRSGGHD+EGLSY++   PFVV+DL NLRSIT+
Sbjct: 86  VSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITL 145

Query: 148 DVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGIA 207
           DV+N   WVQSGAT+GELYY I K S++LAFPAG  PTVG+GG   GGGYG L+RKYG++
Sbjct: 146 DVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLS 205

Query: 208 ADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLKLVRVPATVTVCEA 267
           AD+VIDA++VDANG   DR+ MGED FWAIRGGGG SF +++ WK++L+ VP+ VTV + 
Sbjct: 206 ADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKV 265

Query: 268 NRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQEGKINPIASFSAMFLGKAE 327
            +T E++ + +IN+WQY+A K+  +L +   L   K TE         ASF  ++LG   
Sbjct: 266 VKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQ--KETE-------VYASFPGLYLGPVS 325

Query: 328 ELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQKEDPFEVLLNRTRLYSEISKIKSD 387
           +L+A++K  F +LGL+  +C E SWIESVL   WF K +  E+L  R R  S   K K D
Sbjct: 326 DLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESMEILAKRKRT-SRSFKGKDD 385

Query: 388 YVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDWETPYSHRADYMYLIGYI 447
           +++E IP ++++ +W R ++ +  L++I+  P+GGKMSE+ D E P+ HR   +Y I Y+
Sbjct: 386 FIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYL 445

Query: 448 VKWKEGGVEAE---KRHLNWIREIYDYMTPFVSKSPRAAYINYRDLDVGTNNKYG-KTSY 507
             W E   + +   +++L W+  +Y++MTP+VSKSPR AY+N+RD+D+G       KT Y
Sbjct: 446 AYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKY 505

Query: 508 RRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTLASVSE 546
             A+VWG+KYF NNFDRLV VKT VDP DFF  EQSIP +  V++
Sbjct: 506 EEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIMKYVND 534

BLAST of HG10021460 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 3.1e-144
Identity = 256/541 (47.32%), Postives = 367/541 (67.84%), Query Frame = 0

Query: 10  FGSIAFTIIIIVLS-TTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTPTNSSYSSIL 69
           F S  FTI++  L+ +   S        F++CL +  + NT     V++T  +SS+ SIL
Sbjct: 3   FSSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVE-PKVIHTSKDSSFFSIL 62

Query: 70  NFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS- 129
           + SIQN RF   ETPKP++IITP   S +Q  + C++ HG  +RTRS GH +EGLSYI+ 
Sbjct: 63  DSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAY 122

Query: 130 DVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGG 189
           + PF V+DL NLRSI++DV+N   WVQ+GAT GELYY I K +++LAFPAG  PTVGVGG
Sbjct: 123 NKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGG 182

Query: 190 HLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVD 249
             SGGGYG L+RKYG+AAD++IDA +VDA+G++ DR++MGED FWAIRGGGG SFG+I+ 
Sbjct: 183 QFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILS 242

Query: 250 WKLKLVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISLTGGKVTEDQEG 309
           WK+KLV VP+T+TV +  +T +++ +++I +WQY A K+  +L +       + T ++  
Sbjct: 243 WKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSN 302

Query: 310 KINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQKEDPFEV 369
           K    A F+ +++G    L+A+++  F +LGL+KE C E SWIESVL    F K +   V
Sbjct: 303 KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGV 362

Query: 370 LLNRTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDW 429
           L NR R  S   K K D+V+E IP ++++ +W RL++ +  L +I+  P+GGKMSE+ ++
Sbjct: 363 LTNRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEY 422

Query: 430 ETPYSHRADYMYLIGYIVKWKE----GGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYIN 489
           ETP+ HR   +Y I Y+  W+E       E +K +L W+  +Y++MTP+VSKSPR AY+N
Sbjct: 423 ETPFPHRGGNLYEIQYVAYWREEEDKNKTETDK-YLKWVDSVYEFMTPYVSKSPRGAYVN 482

Query: 490 YRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPTLASV 545
           ++D+D+G      KT Y   + WG+KYF NNF+RLV VKT+VDP+DFF  EQSIP +  V
Sbjct: 483 FKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNKV 533

BLAST of HG10021460 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 499.2 bits (1284), Expect = 4.2e-141
Identity = 253/542 (46.68%), Postives = 362/542 (66.79%), Query Frame = 0

Query: 15  FTIIIIVLS---TTTTSFCTQESNSFVQCLFHYGSPNTN---PISSVVYTPTN--SSYSS 74
           F+I+++  S    T TS      + F+ C+      NT+   P+    + PT   S +S 
Sbjct: 10  FSILVLYFSLYTITPTSSLASLQDQFINCV----QRNTHVYFPLEKTFFAPTKNVSMFSQ 69

Query: 75  ILNFSIQNLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYI 134
           +L  + QNLRFL K  PKP  I +P H SH+QA++ICSK     +R RSGGHD+EGLSY+
Sbjct: 70  VLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYV 129

Query: 135 S--DVPFVVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVG 194
           S  D PF+++DL  +R + I++++ +AWVQSGAT+GELYYRIA+KS+   FPAG C ++G
Sbjct: 130 SQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLG 189

Query: 195 VGGHLSGGGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 254
           +GGH++GG YG ++RKYG+ AD+V+DA +VDANGK+ DR +MGED FWAIRGG GGSFGI
Sbjct: 190 IGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGI 249

Query: 255 IVDWKLKLVRVPATVTVCEANRTLEEDT-MKLINEWQYVAPKLDANL----LLGISLTGG 314
           I+ WK+KLV VP TVTV    +TL++D   K+I++WQ VA KL   L    L  ++ TGG
Sbjct: 250 ILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGG 309

Query: 315 KVTEDQEGKINPIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWF 374
             T           S++A+FLG    LM ++K +F +LGL  +DC+E SW+ES+  +  F
Sbjct: 310 NKT--------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 369

Query: 375 QKEDPFEVLLNRTRLYSEIS-KIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYG 434
               P  VLL     + ++S K KSD+VK  IP S L+G++++L  +D+ L  +++ PYG
Sbjct: 370 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 429

Query: 435 GKMSEVYDWETPYSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPR 494
           G M+++ + + P+ HR   ++ + Y+  W +   +   RH+NWIR++Y YMTP+VS +PR
Sbjct: 430 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSSNPR 489

Query: 495 AAYINYRDLDVGTNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIP 541
            AY+NYRDLD+G N K  KT  ++A+VWG  YF NNF+RL+ +K KVDP +FFRHEQSIP
Sbjct: 490 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of HG10021460 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-139
Identity = 251/529 (47.45%), Postives = 358/529 (67.67%), Query Frame = 0

Query: 16  TIIIIVLSTTTTSFCTQESNSFVQCLFHYGSPNTNPISSVVYTP--TNSSYSSILNFSIQ 75
           T++++ LS    SF T + + FV CL    S  + P+ S  +T     SS+   L  S Q
Sbjct: 15  TLLLLSLSYIPLSFSTIQQD-FVMCLVD-NSDASFPMDSSFFTHDLNASSFKLALETSAQ 74

Query: 76  NLRFLTKETPKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISDV--PF 135
           NLR+L    PKP  I  P + +H+QAAV+C+K     +R RSGGHD+EGLSY+S++   F
Sbjct: 75  NLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAF 134

Query: 136 VVVDLINLRSITIDVENENAWVQSGATLGELYYRIAKKSQTLAFPAGSCPTVGVGGHLSG 195
           V+VDL  LR I++D+E+ +AWV +GA++GE+YYRI +KS+   FPAG C ++G+GGH+ G
Sbjct: 135 VIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIG 194

Query: 196 GGYGWLIRKYGIAADHVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIIVDWKLK 255
           G YG ++RK+G+ AD+V+DA +VDA+GK+ +R +MGED+FWAIRGGGGGSFG+I+ WK+K
Sbjct: 195 GAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIK 254

Query: 256 LVRVPATVTVCEANRTLEEDTMKLINEWQYVAPKLDANLLLGISL-TGGKVTEDQEGKIN 315
           LV VP  VTV    RTLE+D  KL+ +WQ VA KLD +L + + +    K  + +E  I+
Sbjct: 255 LVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTIS 314

Query: 316 PIASFSAMFLGKAEELMAILKPTFSQLGLKKEDCLETSWIESVLMMGWFQKEDPFEVLLN 375
              S+   FLG A  L+ +++ +F QLGL K+DCLETSWI+SV+ +  F    P E LL+
Sbjct: 315 --TSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 374

Query: 376 RTRLYSEISKIKSDYVKERIPNSSLKGMWERLKSQDVELSQIMFVPYGGKMSEVYDWETP 435
              L+    K KSDYV+E IP   L+G+WE+L  +D  L+  ++ PYGG M+++ + ETP
Sbjct: 375 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETP 434

Query: 436 YSHRADYMYLIGYIVKWKEGGVEAEKRHLNWIREIYDYMTPFVSKSPRAAYINYRDLDVG 495
           + HR+  ++ I ++  W++G   +E +H+ W+RE+Y YM  +VSKSPR+AY+NYRDLD+G
Sbjct: 435 FPHRSGTLFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 494

Query: 496 TNNKYGKTSYRRARVWGLKYFGNNFDRLVYVKTKVDPSDFFRHEQSIPT 540
            N K        AR WG +YF  NF+RLV +K K DP +FFRHEQSIPT
Sbjct: 495 MNGKGSD-----AREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531